CN102726521A - Simplified preparation method of low-lactose milk powder - Google Patents
Simplified preparation method of low-lactose milk powder Download PDFInfo
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- CN102726521A CN102726521A CN2012101269995A CN201210126999A CN102726521A CN 102726521 A CN102726521 A CN 102726521A CN 2012101269995 A CN2012101269995 A CN 2012101269995A CN 201210126999 A CN201210126999 A CN 201210126999A CN 102726521 A CN102726521 A CN 102726521A
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Abstract
The invention provides a simplified preparation method of low-lactose milk powder, and belongs to the technical field of dairy product production. According to the invention, a pasteurizing process and a lactose degradation process of the milk are carried out synchronically. In the initial stage of the pasteurizing process of the milk, lactase is added into the milk, and the enzyme hydrolysis process and the pasteurizing process are carried out simultaneously. The equipment used for common milk production processes is not increased, such that equipment investment is relatively reduced. Also, the chance for secondary pollution is reduced. Because the enzyme has a characteristic of high activity, the lactose hydrolysis process is achieved simultaneously during the pasteurizing process. Therefore, the production period of the low-lactose milk is shortened, and the production efficiency is improved. With the enzyme, during the lactose hydrolysis process, galactooligosaccharide with relatively high yield is obtained, such that the nutritive value of the milk is improved.
Description
Technical field
The present invention relates to dairy products production and processing technical field, specifically a kind of simplification preparation method of low-lactose milk powder.
Background technology
General, the low-lactose milk powder hydrolysis process flow process of low temperature has following defective: 1, and the enzymolysis processing time is long, is generally 5-7 days; 2, because this enzyme non-refractory can not be applied in the milk pasteurize processing procedure; 3, it is that millions of lactase drips equipment that this production technology must increase price, has improved a large amount of inputs of production equipment cost, so the medium and small dairy industry of China can not bear and adds the equipment cost that this equipment brings and can't produce low-lactose milk; 4, this enzyme dropping process is after sterilization, so this technology very easily causes the risk of bacterium secondary pollution, causes potential hazard for milk factory and drinking person; 5, this enzyme is an endocellular enzyme, so purity is not high, introduces more foreign protein to milk; 6, stability is not high under this enzyme room temperature, makes troubles for storage and application facet; 7, a lot of enzymatic protective reagents are arranged in this enzyme preparation, these can give the milk quality deleterious impact.
Summary of the invention
Technical assignment of the present invention is the deficiency that solves prior art, and a kind of simplification preparation method of low-lactose milk powder is provided.
Technical scheme of the present invention realizes by following mode, the simplification preparation method of this a kind of low-lactose milk powder, and this preparation method's step is:
(1) raw material milk check: physical and chemical index and microbiological indicator according to GB detect;
(2) clean breast: newborn only through conventional 75% alcohol testing impurity degree≤2.5ppm;
(3) secondary degerming:, increase the gemma clearance through the two times centrifugal degerming;
(4) standardization: the raw material milk for after the clean newborn degreasing carries out the standardization of protein, fat etc.;
(5) the synchronous lactose enzymolysis of pasteurization: the initialization phase at pasteurization adds lactase; The addition of lactase is that lactase and raw material milk volume ratio are 0.6/1000～1.2/1000, and enzymolysis and pasteurization carry out simultaneously, and pasteurizing temperature is controlled at 83 ℃; Time is 15s; Make bacterial population be reduced to 10%, after be cooled to 37 ℃ and keep enzyme digestion reaction, holding time is 2.5 hours;
(6) batching:, change material and add according to used material characteristic;
(7) even matter: even matter pressure 18MPa, even matter temperature is 45 ℃;
(8) preheating sterilization: temperature is 88 ℃, and the time is 30s;
(9) evaporation and concentration: concentrated temperature of charge is 65 ℃, and the dry concentration requirement is 45%;
(10) spray-drying: EAT is controlled at about 180 ℃, and temperature of outgoing air is controlled at about 80 ℃, and high-pressure pump pressure is controlled at 18MPa;
(11) cooling: the milk powder after spray-dried gets into fluid bed, and the EAT of one-level fluid bed is 50 ℃, and the EAT of secondary fluid bed is 50 ℃, and the cooling air temperature is 19 ℃;
(12) sieve powder: control sieve powder temperature is at 20 ℃, to guarantee the flowability of powder;
(13) packing: space sterilization 10 minutes, personnel benefit from 75% alcohol sterilization, and work clothes shone 30 minutes with uviol lamp, packed warehouse-in and outbound again.
The simplification preparation method of this a kind of low-lactose milk powder, the step of optimizing this preparation method is:
(1) raw material milk check: physical and chemical index and microbiological indicator according to GB detect;
(2) clean breast: newborn only through conventional 75% alcohol testing impurity degree≤2.5ppm;
(3) secondary degerming:, increase the gemma clearance through the two times centrifugal degerming;
(4) standardization: the raw material milk for after the clean newborn degreasing carries out the standardization of protein, fat etc.;
(5) the synchronous lactose enzymolysis of pasteurization: the initialization phase at pasteurization adds lactase; The addition of lactase is that lactase and raw material milk volume ratio are 0.6/1000～1.2/1000; Enzymolysis and pasteurization carry out simultaneously, and the pasteurization sterilization temperature is controlled at 65 ℃～69 ℃, keep 30min; Enzymolysis process and pasteurizing process carry out synchronously in this 30min, make bacterial population be reduced to 10%;
(6) batching:, change material and add according to used material characteristic;
(7) even matter: even matter pressure 18MPa, even matter temperature is 45 ℃;
(8) preheating sterilization: temperature is 88 ℃, and the time is 30s;
(9) evaporation and concentration: concentrated temperature of charge is 65 ℃, and the dry concentration requirement is 45%;
(10) spray-drying: EAT is controlled at about 180 ℃, and temperature of outgoing air is controlled at about 80 ℃, and high-pressure pump pressure is controlled at 18MPa;
(11) cooling: the milk powder after spray-dried gets into fluid bed, and the EAT of one-level fluid bed is 50 ℃, and the EAT of secondary fluid bed is 50 ℃, and the cooling air temperature is 19 ℃;
(12) sieve powder: control sieve powder temperature is at 20 ℃, to guarantee the flowability of powder;
(13) packing: space sterilization 10 minutes, personnel benefit from 75% alcohol sterilization, and work clothes shone 30 minutes with uviol lamp, packed warehouse-in and outbound again.
Pasteurization processes is carried out the application on dairy products, dairy produce and beverage, making food synchronously with the degradation process of milk liquid lactose.
The beneficial effect that the present invention is compared with prior art produced is:
This method is utilized thermostable lactase hydrolysed milk lactose technology, has overcome the problem that exists in the above technology like this.At first, use the high temperature resistant beta-glucosidase of pyrococcus furiosus CelB gene expression.This endonuclease capable reduces lactose, and under high temperature and cryogenic conditions, all possesses very strong active.It can catalyzing hydrolysis be incorporated into the β-D-glycosidic bond of terminal irreducibility, discharges aglucon and glucose body simultaneously.The high temperature beta-glucosidase not only has glucosidase activity, but also has effect (Km=2.3mm, the Kcat (s of very strong galactosidase
-1)=2050 ± 86),, can be applicable to dairy industry and reduce lactose, lactose is decomposed into galactooligosaccharide and glucose so high temperature resistant beta-glucosidase is also referred to as lactase.This enzyme still has very strong stability under 100 ℃; And under the lower temperature about 30 ℃, still can keep very strong 40% activity, because beta-glucosidase has stability in temperature range widely, therefore multiple addition manner is provided for dairy industry production low-lactose milk; Can when the dairy products pasteurization, add synchronously; Reduce the chance of secondary pollution, can add when the low temperature according to the requirement of dairy products in addition, still can reach desirable effect.High temperature resistant this advantage is that dairy industry provides the good approach of producing low-lactose milk powder simultaneously.
Add this enzyme hydrolysis lactose during the pasteurization of this method synchronously, can not increase the used production equipment of common milk producing technology, reduced the input of equipment relatively; Reduced the chance of secondary pollution; Because these enzyme high activity characteristics, promptly accomplished the hydrolytic process of lactose simultaneously at pasteurization, shortened the production cycle of low-lactose milk like this, improved production efficiency; This enzyme hydrolyzes lactose can produce simultaneously high yield galactooligosaccharide, increased the nutritive value of milk.
This method adopts advanced biological enzymolysis technology; Develop a kind of technological process of producing low-lactose milk powder of simplifying; Lactose hydrolysis ratio can reach more than 80%; Milk powder is nutritious comprehensively, not only can play to alleviate and improve the intolerance shape of lactose intolerance crowd to lactose, and can satisfy the demand of this type of crowd to local flavor and mouthfeel.The preparation method of low-lactose milk powder of the present invention is a core with the synchronous pasteurising step of lactase hydrolysis; Research through batching, filtration, even matter, preheating sterilization, evaporation and concentration, spray-drying, cooling, sieve powder, packaging and other steps process conditions; Through the synchronous pasteurization of lactase hydrolysis, not only reduced the bacterial population in the raw material milk, and reduced the content of lactose in the product; Proposed the preparation method of optimal parameter condition, improved utilization rate milk.
The specific embodiment
Simplification preparation method in the face of a kind of low-lactose milk powder of the present invention does following detailed description down.
Milk powder parameter after the conventional milk powder production and processing:
| Project | Index (per 100 grams) |
| Protein (g) | 18.0 |
| Fat (g) | 18.0 |
| Carbohydrate (g) | 55.0 |
| Lactose (g) | 38.5 |
[0040]Embodiment one:
1. raw material milk check: according to GB GB/T6914 (physical and chemical index: fat>=3.1%; Protein>=2.95%; Acidity≤0.162% (representing) with lactic acid; Microbiological indicator: total number of bacteria≤200,000/ml) detect;
2. clean breast: impurity degree≤2.5ppm, through conventional 75% alcohol testing;
3. secondary degerming: through the two times centrifugal degerming, a degerming makes the clearance of gemma reach 95% of gemma sum, and the secondary degerming makes the clearance of gemma reach 99% of gemma sum;
4. standardization: the raw material milk for after the clean newborn degreasing carries out the standardization of protein, fat etc.;
5. pasteurization: the synchronous lactose enzymolysis of pasteurization: the initialization phase at pasteurization adds lactase; The addition of lactase is that lactase and raw material milk volume ratio are 0.6/1000～1.2/1000, and enzymolysis and pasteurization carry out simultaneously, and pasteurizing temperature is controlled at 83 ℃; Time is 15s; Make bacterial population be reduced to 10%, after be cooled to 37 ℃ and keep enzyme digestion reaction, holding time is 2.5 hours;
6. prepare burden:, change material and add according to used material characteristic;
7. even matter: even matter pressure 18MPa, even matter temperature is 45 ℃;
8. preheating sterilization: temperature is 88 ℃, and the time is 30s;
9. evaporation and concentration: concentrated temperature of charge is 65 ℃, and the dry concentration requirement is 45%;
10. spray-drying: EAT is controlled at about 180 ℃, and temperature of outgoing air is controlled at about 80 ℃, and high-pressure pump pressure is controlled at 18MPa;
11. cooling: the milk powder after spray-dried gets into fluid bed, and the EAT of one-level fluid bed is 50 ℃, and the EAT of secondary fluid bed is 50 ℃, and the cooling air temperature is 19 ℃;
12. sieve powder: control sieve powder temperature is at 20 ℃, to guarantee the flowability of powder;
13. packing: space sterilization 10 minutes, personnel benefit from 75% alcohol sterilization, and work clothes shone 30 minutes with uviol lamp, packed warehouse-in and outbound again.
| Project | Index (per 100 grams) |
| Protein (g) | 17.8 |
| Fat (g) | 18.2 |
| Carbohydrate (g) | 54.0 |
| Lactose (g) | 8.0 |
Embodiment two:
1. raw material milk check: according to GB GB/T6914 (physical and chemical index: fat>=3.1%; Protein>=2.95%; Acidity≤0.162% (representing) with lactic acid; Microbiological indicator: total number of bacteria≤200,000/ml) detect;
2. clean breast: impurity degree≤2.5ppm, through conventional 75% alcohol testing;
3. secondary degerming: through the two times centrifugal degerming, a degerming makes the clearance of gemma reach 95% of gemma sum, and the secondary degerming makes the clearance of gemma reach 99% of gemma sum;
4. standardization: the raw material milk for after the clean newborn degreasing carries out the standardization of protein, fat etc.;
5. the synchronous lactose enzymolysis of pasteurization: the initialization phase at pasteurization adds lactase; The addition of lactase is that lactase and raw material milk volume ratio are 0.6/1000～1.2/1000; Enzymolysis and pasteurization carry out simultaneously, and the pasteurization sterilization temperature is controlled at 65 ℃～69 ℃, keep 30min; Enzymolysis process and pasteurizing process carry out synchronously in this 30min, make bacterial population be reduced to 10%;
6. prepare burden:, change material and add according to used material characteristic;
7. even matter: even matter pressure 18MPa, even matter temperature is 45 ℃;
8. preheating sterilization: temperature is 88 ℃, and the time is 30s;
9. evaporation and concentration: concentrated temperature of charge is 65 ℃, and the dry concentration requirement is 45%;
10. spray-drying: EAT is controlled at about 180 ℃, and temperature of outgoing air is controlled at about 80 ℃, and high-pressure pump pressure is controlled at 18MPa;
11. cooling: the milk powder after spray-dried gets into fluid bed, and the EAT of one-level fluid bed is 50 ℃, and the EAT of secondary fluid bed is 50 ℃, and the cooling air temperature is 19 ℃;
12. sieve powder: control sieve powder temperature is at 20 ℃, to guarantee the flowability of powder;
13. packing: space sterilization 10 minutes, personnel benefit from 75% alcohol sterilization, and work clothes shone 30 minutes with uviol lamp, packed warehouse-in and outbound again.
| Project | Index (per 100 grams) |
| Protein (g) | 18.0 |
| Fat (g) | 17.5 |
| Carbohydrate (g) | 56.0 |
| Lactose (g) | 7.5 |
The project testing result contrast of the milk powder of process embodiment one, embodiment two and routine; Obviously, through the synchronous pasteurising step of enzyme hydrolysis, not only can prepare lactose content and reduce greatly; The milk powder of its nutrient content unchanged; And, simplified the production technology of low-lactose milk powder, greatly reduced the microbiological contamination probability in production cost and the production process.Low-lactose milk powder is nutritious comprehensively, not only can play to alleviate and improve the lactose intolerance symptom, but also can satisfy the demand of this type of crowd to local flavor and mouthfeel.
Claims (3)
1. the simplification preparation method of a low-lactose milk powder is characterized in that this preparation method's step is:
(1) raw material milk check: physical and chemical index and microbiological indicator according to GB detect;
(2) clean breast: newborn only through conventional 75% alcohol testing impurity degree≤2.5ppm;
(3) secondary degerming:, increase the gemma clearance through the two times centrifugal degerming;
(4) standardization: the raw material milk for after the clean newborn degreasing carries out the standardization of protein, fat etc.;
(5) the synchronous lactose enzymolysis of pasteurization: the initialization phase at pasteurization adds lactase; The addition of lactase is that lactase and raw material milk volume ratio are 0.6/1000～1.2/1000, and enzymolysis and pasteurization carry out simultaneously, and pasteurizing temperature is controlled at 83 ℃; Time is 15s; Make bacterial population be reduced to 10%, after be cooled to 37 ℃ and keep enzyme digestion reaction, holding time is 2.5 hours;
(6) batching:, change material and add according to used material characteristic;
(7) even matter: even matter pressure 18MPa, even matter temperature is 45 ℃;
(8) preheating sterilization: temperature is 88 ℃, and the time is 30s;
(9) evaporation and concentration: concentrated temperature of charge is 65 ℃, and the dry concentration requirement is 45%;
(10) spray-drying: EAT is controlled at about 180 ℃, and temperature of outgoing air is controlled at about 80 ℃, and high-pressure pump pressure is controlled at 18MPa;
(11) cooling: the milk powder after spray-dried gets into fluid bed, and the EAT of one-level fluid bed is 50 ℃, and the EAT of secondary fluid bed is 50 ℃, and the cooling air temperature is 19 ℃;
(12) sieve powder: control sieve powder temperature is at 20 ℃, to guarantee the flowability of powder;
(13) packing: space sterilization 10 minutes, personnel benefit from 75% alcohol sterilization, and work clothes shone 30 minutes with uviol lamp, packed warehouse-in and outbound again.
2. the simplification preparation method of a low-lactose milk powder is characterized in that this preparation method's step is:
(1) raw material milk check: physical and chemical index and microbiological indicator according to GB detect;
(2) clean breast: newborn only through conventional 75% alcohol testing impurity degree≤2.5ppm;
(3) secondary degerming:, increase the gemma clearance through the two times centrifugal degerming;
(4) standardization: the raw material milk for after the clean newborn degreasing carries out the standardization of protein, fat etc.;
(5) the synchronous lactose enzymolysis of pasteurization: the initialization phase at pasteurization adds lactase; The addition of lactase is that lactase and raw material milk volume ratio are 0.6/1000～1.2/1000; Enzymolysis and pasteurization carry out simultaneously, and the pasteurization sterilization temperature is controlled at 65 ℃～69 ℃, keep 30min; Enzymolysis process and pasteurizing process carry out synchronously in this 30min, make bacterial population be reduced to 10%;
(6) batching:, change material and add according to used material characteristic;
(7) even matter: even matter pressure 18MPa, even matter temperature is 45 ℃;
(8) preheating sterilization: temperature is 88 ℃, and the time is 30s;
(9) evaporation and concentration: concentrated temperature of charge is 65 ℃, and the dry concentration requirement is 45%;
(10) spray-drying: EAT is controlled at about 180 ℃, and temperature of outgoing air is controlled at about 80 ℃, and high-pressure pump pressure is controlled at 18MPa;
(11) cooling: the milk powder after spray-dried gets into fluid bed, and the EAT of one-level fluid bed is 50 ℃, and the EAT of secondary fluid bed is 50 ℃, and the cooling air temperature is 19 ℃;
(12) sieve powder: control sieve powder temperature is at 20 ℃, to guarantee the flowability of powder;
(13) packing: space sterilization 10 minutes, personnel benefit from 75% alcohol sterilization, and work clothes shone 30 minutes with uviol lamp, packed warehouse-in and outbound again.
3. pasteurization processes according to claim 1 and 2 is carried out the application on dairy products, dairy produce and beverage, making food synchronously with the degradation process of milk liquid lactose.
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Cited By (6)
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| CN103385292A (en) * | 2013-08-12 | 2013-11-13 | 新疆西部牧业股份有限公司 | Method for processing infant milk powder by wet process |
| CN104542990A (en) * | 2015-01-28 | 2015-04-29 | 贝因美婴童食品股份有限公司 | Processing technique of milk powder |
| CN106804706A (en) * | 2015-11-30 | 2017-06-09 | 内蒙古伊利实业集团股份有限公司 | A kind of low-lactose milk powder and preparation method thereof |
| CN107432323A (en) * | 2017-01-22 | 2017-12-05 | 云南新希望邓川蝶泉乳业有限公司 | A kind of low-lactose milk powder and preparation method thereof |
| CN107788129A (en) * | 2017-11-17 | 2018-03-13 | 荣成奥汛海洋生物科技有限公司 | A kind of functional food for promoting children's growth and preparation method thereof |
| US11503836B2 (en) * | 2020-10-20 | 2022-11-22 | Michael J. McCloskey | Process for making lactose-free milk powders |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103385292A (en) * | 2013-08-12 | 2013-11-13 | 新疆西部牧业股份有限公司 | Method for processing infant milk powder by wet process |
| CN104542990A (en) * | 2015-01-28 | 2015-04-29 | 贝因美婴童食品股份有限公司 | Processing technique of milk powder |
| CN106804706A (en) * | 2015-11-30 | 2017-06-09 | 内蒙古伊利实业集团股份有限公司 | A kind of low-lactose milk powder and preparation method thereof |
| CN107432323A (en) * | 2017-01-22 | 2017-12-05 | 云南新希望邓川蝶泉乳业有限公司 | A kind of low-lactose milk powder and preparation method thereof |
| CN107788129A (en) * | 2017-11-17 | 2018-03-13 | 荣成奥汛海洋生物科技有限公司 | A kind of functional food for promoting children's growth and preparation method thereof |
| US11503836B2 (en) * | 2020-10-20 | 2022-11-22 | Michael J. McCloskey | Process for making lactose-free milk powders |
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Application publication date: 20121017 |