CN102706717B - Moth grinding sample preparation system for detecting silkworm female moth corpuscule pathogeny spore - Google Patents
Moth grinding sample preparation system for detecting silkworm female moth corpuscule pathogeny spore Download PDFInfo
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- CN102706717B CN102706717B CN201210232895.2A CN201210232895A CN102706717B CN 102706717 B CN102706717 B CN 102706717B CN 201210232895 A CN201210232895 A CN 201210232895A CN 102706717 B CN102706717 B CN 102706717B
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Abstract
The invention relates to a moth grinding sample preparation system for detecting silkworm female moth corpuscule pathogeny spore. The system is composed of a moth grinding system 1, an automatic liquid relief system 2, a centrifugal enrichment device 3, a centrifugal tube washing device 4 and an automatic washing system 5, and the moth grinding system 1, the automatic liquid relief system 2, the centrifugal enrichment device 3, the centrifugal tube washing device 4 and the automatic washing system 5 are integrated on a working table board sequentially. The moth grinding sample preparation system basically achieves mechanical operation of various links of moth grinding and sample preparation and saves labor. Liquid injection and moth grinding are conducted synchronously, contact chances between materials and blades are increased, and grinding of the materials is thorough. Electromagnetic automatic liquid relief (imbibition and liquid-returning) is adopted, and return liquid quantity is even and accurate. A centrifugal tube is washed by using an automatic transmission spray-washing device, links of moth grinding, imbibition, liquid-returning and washing of the centrifugal tube are covered, and automatic spray-washing is achieved. A high-pressure automatic washing device is adopted, water-saving effect is remarkable, and 80% of water amount can be saved. The moth grinding sample preparation system has the advantages of being high in work efficiency, water-saving, good in safety and high in sample definition. The moth grinding sample preparation system can be applied to detection of the silkworm female moth corpuscule pathogeny spore through a microscope for silkworm egg production units and silkworm egg quality inspection departments, and samples can be prepared fast and massively.
Description
Technical field
The present invention relates to agricultural machinery technological field, particularly relate to a kind of mill moth sample-preparing system for detection of the female moth granulosis of silkworm protospore.
Background technology
Pebrine disease is a kind of infectiousness flacherie very strong to silkworm industry harmfulness, 19th-century mid-term (1845-1865) once made European silkworm industry be on the verge of to destroy, microbiologist's Pasteur research in 1865 to 1870 is found, granulosis protospore has embryo and infects, from the female moth of previous generation, infect the characteristic of harm filial generation, invented the check of single moth egg production of hybrid seeds, single moth, rejected female moth inspection technology of disease ovum, this technology has been saved Europe and world's silkworm industry.Along with production-scale expansion, test samples enormous amount, be difficult to one-hundred-percent inspection, nineteen sixty judges that for the large island of Japanese scientist lattice (1960-1965) application sample technique and probability single moth check is become to group (30 moth) to be checked, and invented group's grinding machine, adopt high speed electric reamer to grind female moth, after then filtering by manual work, prepare check sample.At the beginning of the 1980's, group's grinding machine and applied probability sampling decision technology are successively introduced and made by oneself in China Sichuan, Guangdong, Jiangsu and Zhejiang, uses the present always.The flow process of using grinding machine to prepare sample is at present that female moth of sampling is put into after material cup, by fluid injection, mill moth, standing, filtration, centrifugation and vibration spot film process, except mill moth, other operations are all manually to complete, the effort of taking a lot of work, cannot realize the rapid extraction of a plurality of samples, and material cup and centrifuge tube rinse with manual operation tap water after using, water consumption is large, wastes very surprising.Up to now, silkworm egg is produced preparation that female moth granulosis protospore detects sample and is only rested on machinery and grind female moth stage, and mechanical development work and the system integration of subsequent operation flow process there is no new progress both at home and abroad.
Summary of the invention
For the problems referred to above, the object of this invention is to provide a kind of mill moth sample-preparing system for detection of the female moth granulosis of silkworm protospore, to realize rapid batch, detect the preparation of sample.This system is synchronously carried out fluid injection and mill moth, standing and filtration manual operations changes automatic sucking into, moves liquid, the washer automatic washing of transmission for centrifuge tube, design hydraulic devices and solenoid valve, cover mill moth, absorption, centrifuge tube washs each link, realizes the automatic spray-washing to material cup and various utensils, and mill moth, sampling, centrifugal, cleaning device are integrated in to a work top, realize the preparation that rapid batch detects sample.
For achieving the above object, the present invention takes following technology path: 1) simple mill moth device of past is improved, fluid injection and mill moth synchronously carry out; 2) with automatic imbibition, return liquid device and replace manual operations; 3) with centrifuge tube automatic flushing device, replace running water pipe artificial flushing; 4) design hydraulic devices and solenoid valve, covering mill moth, absorption, centrifuge tube wash each link, realize the automatic spray-washing to material cup and various utensils; 5) mill moth, imbibition are returned to liquid, centrifugal, cleaning device is integrated in a work top.
A kind of mill moth sample-preparing system for detection of the female moth granulosis of silkworm protospore of the present invention, by mill moth system 1, automatic liquor-transferring system 2, centrifugal enrichment device 3, centrifuge tube wash mill 4 and automatic cleaning system 5, formed, and be integrated in successively a work top;
Mill moth system 1 comprises operation starting switch 1-1, cutter head 1-2, cone circle 1-3, protective shield 1-4, lye tank 1-5, liquid injection pipe 1-6 and motor 1-7, lye tank 1-5 is positioned at mill moth system 1 top and is connected with liquid injection pipe 1-6 by solenoid valve, cutter head 1-2, cone circle 1-3 are connected with motor 1-7, and protective shield 1-4 is arranged at before cutter head 1-2;
Automatically liquor-transferring system 2 comprises suction pumps 2-1, suction nozzle 2-2, the first cylinder transmission device 2-3a, the second cylinder transmission device 2-3b and the 3rd cylinder transmission device 2-3c, electromagnetic turnover 2-4, slide rail 2-5 and air compressor 2-6; Suction pumps 2-1 is connected with suction nozzle 2-2 by pipeline, solenoid valve, suction nozzle 2-2 top is fixed on the piston rod of the first cylinder transmission device 2-3 a, move up and down, suction nozzle 2-2 connects with the second cylinder transmission device 2-3b and the 3rd cylinder transmission device 2-3c in the horizontal direction, and by sliding before and after slide rail 2-5;
Centrifugal enrichment device 3 is conventional high speed hydro-extractor, for the centrifugal enrichment of subject matter to be detected;
Centrifuge tube wash mill 4 comprises gear turntable 4-1, speed reduction unit 4-2 and motor 4-3, and motor 4-3 is connected with gear turntable 4-1 by speed reduction unit 4-2, and gear turntable 4-1 rotational speed or test tube travelling speed regulate by speed reduction unit 4-2;
Automatic cleaning system 5 comprises draws together water pump 5-4, pipeline, solenoid valve, the first shower nozzle 5-1, the second shower nozzle 5-2a, the 3rd shower nozzle 5-2b and the 4th shower nozzle 5-3; The first shower nozzle 5-1 is positioned under the cutter head 1-2 of mill moth system 1, the second shower nozzle 5-2a is positioned under the electromagnetic turnover 2-4 of automatic liquor-transferring system 2, the 3rd shower nozzle 5-2b is positioned under the suction nozzle 2-2 of automatic liquor-transferring system 2, the 4th shower nozzle 5-3 is positioned at below the gear turntable 4-1 of centrifuge tube wash mill 4, mates with centrifuge tube running orbit.
Further, cutter head 1-2, suction nozzle 2-2, the first shower nozzle 5-1, the second shower nozzle 5-2a and the 3rd shower nozzle 5-2b are 4, and word order, the 4th shower nozzle 5-3 is 4, be evenly distributed on the circular arc of centrifuge tube running orbit, the top of cutter head 1-2 is provided with pressing plate, with fixed electrical machinery sealants cup;
Further, automatically the suction nozzle 2-2 of liquor-transferring system 2 is communicated with respectively 4 separate pipelines, before approaching suction pumps 2-1, converge into a pipeline, by solenoid valve, the female moth of the silkworm having ground in material cup is ground in liquid upstream line, article 4, the capacity of pipeline is enough to the female moth of silkworm that splendid attire draws and grinds liquid, and leave larger surplus capacity, to avoid mutual pollution.
Accompanying drawing explanation
fig. 1 ismill moth sample-preparing system schematic diagram
1, mill moth system, 2, liquor-transferring system automatically, 3, centrifugal enrichment device, 4, centrifuge tube wash mill, 5, automatic cleaning system.I, be water inlet direction, II, for water outlet direction.
fig. 2 ismill moth system diagram
1-1, operation starting switch, 1-2, cutter head, 1-3, cone circle, 1-4, protective shield, 1-5, lye tank, 1-6, liquid injection pipe, 1-7, motor, 5-1, the first shower nozzle
fig. 3 isautomatic moving liquid system diagram, wherein A is left view, and B is front view, and C is vertical view
2-1, suction pumps, 2-2, suction nozzle, 2-3a, the first cylinder transmission device, 2-3b, the second cylinder transmission device, 2-3c the 3rd cylinder transmission device, 2-4, electromagnetic turnover, 2-5, slide rail, 2-6, air compressor, 5-2a, the second shower nozzle, 5-2b, the 3rd shower nozzle, 5-4, water pump
fig. 4 iscentrifuge tube wash mill figure
4-1, gear turntable, 4-2, speed reduction unit, 4-3, motor, 5-3, the 4th shower nozzle
Advantage of the present invention is:
1) the present invention by fluid injection grind moth, move liquid (liquid is returned in imbibition), centrifugal some plate, test tube clean and be integrated in a worktable, substantially realized the mechanically actuated of each link of mill moth sample preparation, save labor;
2) fluid injection is synchronizeed and is carried out with mill moth, has increased the chance that material contacts with blade, and material grinds more thorough; On the other hand, adopt electromagnetism automatic moving liquid (liquid is returned in imbibition), time liquid measure is evenly accurate;
3) adopt high-pressure auto washing utensil, water-saving result highly significant, result shows can reduce water consumption 80%;
specific implementation method
Embodiment
As shown in Figure 1, mill moth system 1, automatic liquor-transferring system 2, centrifugal enrichment device 3, centrifuge tube wash mill 4 and automatic cleaning system 5 are integrated into a work top; Whole work top 3.5m * 1.2 m, Wei Zhuane district, the rightmost side, is then followed successively by mill moth system 1, automatic liquor-transferring system 2, centrifugal enrichment device 3, centrifuge tube wash mill 4 and automatic cleaning system 5, Wei Dian section, the leftmost side; Under table top, be pipeline, water pump and the shower nozzle etc. of automatic cleaning system 5;
Described each ingredient course of work of mill moth sample-preparing system is as follows, and Zhuan E district packs material cup into the female moth of silkworm, and then mobile material cup is to mill moth system 1; After mill moth completes, mobile material cup, to automatic liquor-transferring system 2, is pipetted into centrifuge tube by sample, and then electromagnetic turnover rotates material cup is cleaned, and automatically cleans transfer pipet simultaneously; Then centrifuge tube is put into centrifugal enrichment device 3 and is carried out centrifugal enrichment; After centrifugal point sample, centrifuge tube cleans by centrifuge tube wash mill 4; The utensil of each working portion cleans and is completed by automatic cleaning system 5.
As shown in Figure 2, Figure 3 and Figure 4, during this system works, switch on power, the material cup horizontal sliding that the female moth of silkworm is housed is put into mill moth system 1, press operation starting switch 1-1, protective shield 1-4 and cutter head 1-2 decline, alkali lye when cutter head 1-2 declines in lye tank 1-5 by solenoid valve through liquid injection pipe 1-6 automatic fluid injection, after cutter head 1-2 decline puts in place, be spin finishing, reservoir quantity and milling time are controlled by the time relay; After mill moth is finished, cutter head 1-2 rises automatically, switches on the pump, and the first shower nozzle 5-1 cleans cutter head 1-2;
Artificial taking-up material cup also places it on the electromagnetic turnover 2-4 of automatic liquor-transferring system 2, press imbibition button, moving liquid work starts, order is as follows: air compressor 2-6 promotes the piston rod of the first cylinder transmission device 2-3a, drive suction nozzle 2-2 decline-suction pumps 2-1 work, after completing by solenoid control suction nozzle 2-2 imbibition-imbibition, air compressor 2-6 promotes the piston rod of the first cylinder transmission device 2-3a, after driving suction nozzle 2-2 to rise and being driven the pressurized air that retreats to thereafter centrifuge tube top-air compressor 2-6 generation by solenoid valve, the liquid of absorption to be pressed out to centrifuge tube-move liquid and to complete by the second cylinder transmission device 2-3b, suction nozzle 2-2 is driven and is retreated to the 3rd shower nozzle 5-2b place-decline-water pump 5-4 work by the 3rd cylinder transmission device 2-3c, the 3rd shower nozzle 5-2b rinses suction nozzle 2-2, water pump 5-4 introduces water imbibition pipeline simultaneously and sprays from suction nozzle 2-2, to clean imbibition pipeline and suction nozzle 2-2 inside-suction nozzle 2-2, rise and advance to initial position, suction nozzle 2-2 is from material cup imbibition, and electromagnetic turnover 2-4, by 180 ° of material cup upsets downwards, is completed the flushing of material cup by the second shower nozzle 5-2a, rinsed rear electromagnetic turnover 2-4 and resetted,
The centrifuge tube that fills the female moth of silkworm and grind liquid is put into centrifugal enrichment device 3 and carry out centrifugal enrichment; Centrifuge tube back-off after use is put into the entrance of centrifuge tube wash mill 4, and motor 4-3 starts, and speed reduction unit 4-2 controls rotating speed, gear turntable 4-1 brings centrifuge tube into the 4th shower nozzle 5-3 top and rinses, cleaning speed is 20-40/minutes, and after automatically cleaning, centrifuge tube skids off from outlet.
Claims (3)
1. the mill moth sample-preparing system for detection of the female moth granulosis of silkworm protospore, it is characterized in that being formed by mill moth system (1), automatic liquor-transferring system (2), centrifugal enrichment device (3), centrifuge tube wash mill (4) and automatic cleaning system (5), and be integrated in successively a work top;
Mill moth system (1) comprises operation starting switch (1-1), cutter head (1-2), cone circle (1-3), protective shield (1-4), lye tank (1-5), liquid injection pipe (1-6) and motor (1-7), lye tank (1-5) is positioned at mill moth system (1) top and is connected with liquid injection pipe (1-6) by solenoid valve, cutter head (1-2), cone circle (1-3) are connected with motor (1-7), and protective shield (1-4) is arranged at cutter head (1-2) above;
Automatically liquor-transferring system (2) comprises suction pumps (2-1), suction nozzle (2-2), the first cylinder transmission device (2-3a), the second cylinder transmission device (2-3b) and the 3rd cylinder transmission device (2-3c), electromagnetic turnover (2-4), slide rail (2-5) and air compressor (2-6); Suction pumps (2-1) is connected with suction nozzle (2-2) by pipeline, solenoid valve, suction nozzle (2-2) top is fixed on the first cylinder transmission device (on 2-3 piston rod a), move up and down, suction nozzle (2-2) is connected with the second cylinder transmission device (2-3b) and the 3rd cylinder transmission device (2-3c) in the horizontal direction, and by sliding before and after slide rail (2-5);
Centrifugal enrichment device (3) is conventional high speed hydro-extractor, for the centrifugal enrichment of subject matter to be detected;
Centrifuge tube wash mill (4) comprises gear turntable (4-1), speed reduction unit (4-2) and motor (4-3), motor (4-3) is connected with gear turntable (4-1) by speed reduction unit (4-2), and gear turntable (4-1) rotational speed or test tube travelling speed regulate by speed reduction unit (4-2);
Automatic cleaning system (5) comprises draws together water pump (5-4), pipeline, solenoid valve, the first shower nozzle (5-1), the second shower nozzle (5-2a), the 3rd shower nozzle (5-2b) and the 4th shower nozzle (5-3); The first shower nozzle (5-1) is positioned under the cutter head (1-2) of mill moth system (1), the second shower nozzle (5-2a) is positioned under the electromagnetic turnover (2-4) of automatic liquor-transferring system (2), the 3rd shower nozzle (5-2b) is positioned under the suction nozzle (2-2) of automatic liquor-transferring system (2), the gear turntable (4-1) that the 4th shower nozzle (5-3) is positioned at centrifuge tube wash mill (4) below, mates with centrifuge tube running orbit.
2. a kind of mill moth sample-preparing system for detection of the female moth granulosis of silkworm protospore as claimed in claim 1, it is characterized in that, cutter head (1-2) suction nozzle (2-2), the first shower nozzle (5-1), the second shower nozzle (5-2a) and the 3rd shower nozzle (5-2b) are 4, and word order, the 4th shower nozzle (5-3) is 4, be evenly distributed on the circular arc of centrifuge tube running orbit, the top of cutter head (1-2) is provided with pressing plate, with fixed electrical machinery sealants cup.
3. a kind of mill moth sample-preparing system for detection of the female moth granulosis of silkworm protospore as claimed in claim 2, it is characterized in that, automatically the suction nozzle (2-2) of liquor-transferring system (2) is communicated with respectively 4 separate pipelines, approaching, suction pumps (2-1) is front converges into a pipeline, by solenoid valve, the female moth of the silkworm having ground in material cup is ground in liquid upstream line, article 4, the capacity of pipeline is enough to the female moth of silkworm that splendid attire draws and grinds liquid, and leave larger surplus capacity, to avoid mutual pollution.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210232895.2A CN102706717B (en) | 2012-07-06 | 2012-07-06 | Moth grinding sample preparation system for detecting silkworm female moth corpuscule pathogeny spore |
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| CN201210232895.2A CN102706717B (en) | 2012-07-06 | 2012-07-06 | Moth grinding sample preparation system for detecting silkworm female moth corpuscule pathogeny spore |
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| CN102706717B true CN102706717B (en) | 2014-08-06 |
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| CN107552213A (en) * | 2017-10-22 | 2018-01-09 | 赵延毓 | A kind of grater examined for silkworm moth |
| CN110567768A (en) * | 2018-06-05 | 2019-12-13 | 安徽省农业科学院蚕桑研究所 | Single moth grinder for detecting silkworm corpuscle disease mother moth |
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| CN101703035A (en) * | 2009-11-09 | 2010-05-12 | 苏州大学 | Method for preparing silkworm eggs for transgene of eggs of bivoltine silkworm varieties |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101703035A (en) * | 2009-11-09 | 2010-05-12 | 苏州大学 | Method for preparing silkworm eggs for transgene of eggs of bivoltine silkworm varieties |
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