CN102703218A - Method for extracting neutral fragrance ingredients from tobacco leaves - Google Patents
Method for extracting neutral fragrance ingredients from tobacco leaves Download PDFInfo
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- CN102703218A CN102703218A CN2012101977664A CN201210197766A CN102703218A CN 102703218 A CN102703218 A CN 102703218A CN 2012101977664 A CN2012101977664 A CN 2012101977664A CN 201210197766 A CN201210197766 A CN 201210197766A CN 102703218 A CN102703218 A CN 102703218A
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Abstract
The invention discloses a method for extracting neutral fragrance ingredients from tobacco leaves. The method comprises the following steps of: performing ultrasonic processing at room temperature by taking the tobacco leaves as raw materials and ethyl acetate as an extraction solvent, standing for a period of time, and filtering to remove tobacco smalls to obtain the neutral fragrance ingredients of alcohol, aldehyde, ketone and ester compounds. Compared with the prior art, the method has the advantages that the toxicity and using amount of the solvent are low, the yield is high, a process is simple, and the repeatability is high. The method is high in extraction efficiency on the neutral fragrance ingredients of the tobacco leaves, and the corresponding neutral fragrance ingredients are not separated out nearly by secondary extraction.
Description
Technical field
The invention belongs to technical field of tobacco, be specifically related to the process for extracting of compound in the tobacco leaf.
Background technology
Tobacco flavor is the important indicator of evaluation tobacco and product property thereof.Neutral flavor compositions such as the alcohol in the tobacco leaf, aldehyde, ketone, ester class are to cigarette flavor and jealous significant contribution is arranged, and mostly these materials be the important component of tobacco essential oil, are widely used in fields such as essence and flavoring agent, tobacco industry.
Mostly tobacco leaf alcohol, aldehyde, ketone, ester class neutral flavor composition are the material of middle polarity.At present, to the tobacco flavor composition unified extractive technique is not arranged both at home and abroad.(comprehensive two dimensional gas chromatography-time-of-flight mass spectrometry (TOFMS) is measured the neutral chemical composition of tobacco to distillation extraction in the time of the most frequently used.Chromatogram, 2007,25 (2): 30-34) need at high temperature extract target component, lower to the neutral flavor composition extraction efficiency of some poor heat stability, method technology is wayward, and side reaction is more, and circulation ratio is relatively poor; This method is bigger for this noxious solvent consumption of methylene dichloride in addition, causes in various degree to environment and pollutes.Steam distillation technology (flavor chemistry and technic.Henry, 1991) be similar to distillation extraction technology simultaneously, need at high temperature extract target components, side reaction is more, and is undesirable for the material extraction efficiency of some poor heat stability.Existing solvent extraction process extracts (Talanta, 2010 like accelerated solvent; 81:650-656) can under low relatively temperature, extract flavour ingredient, but not carry out the reasonably optimizing selection to extracting solvent, selected solvent toxicity is bigger; Solvent load is bigger, and environment is caused pollution to a certain degree.Can know by " the similar principle that mixes "; The extraction solvent extraction of the neutral flavor components utilising middle polarity that polarity is medium has extraction efficiency preferably, and above-mentioned solvent extraction process does not rationally screen, optimizes extracting solvent according to the polarity characteristics and the polarity of solvent of neutral flavor composition self.
The shortcoming of distillation extraction, steam distillation, solvent extraction such as solvent toxicity were big when the novel process that the present invention relates to adopted to existing technology, waste liquid is many, yield is low, complex process, poor repeatability are succeeded in developing.
Summary of the invention
Given this, the object of the present invention is to provide that a kind of process solvent toxicity is little, solvent load is few, yield is high, technology is simple, the process for extracting of the neutral flavor composition of good reproducibility.
For solving the problems of the technologies described above, technical scheme provided by the invention is that a kind of process for extracting of tobacco leaf neutral flavor composition is provided; With the tobacco leaf is raw material; ETHYLE ACETATE is for extracting solvent, and at room temperature supersound process leaves standstill for some time again; Remove by filter offal, obtain the neutral flavor composition of alcohol, aldehyde, ketone, ester compound.
Further, every gram tobacco leaf adapted ETHYLE ACETATE 5～25mL.
Preferably, every gram tobacco leaf adapted ETHYLE ACETATE 10mL.
Further, the supersound process time is lower than 30min.
Preferably, the supersound process time is 5min.
Further, time of repose is lower than 24h.Leaving standstill is to make neutral flavor composition and extraction solvent ethyl acetate that static mass transfer process arranged, thereby improves extraction efficiency.
Preferably, time of repose is 3h.
Compared with prior art, process solvent toxicity of the present invention is little, solvent load is few, yield is high, technology is simple, good reproducibility.Process for extracting of the present invention is higher to tobacco leaf neutral flavor composition extraction efficiency, does not almost analyze corresponding neutral flavor composition through second extraction.
Embodiment
Can further be expressly understood the present invention through specific embodiment of the present invention given below, but following embodiment is not to qualification of the present invention.Employed TP is ordinary method like no specified otherwise among the following embodiment.
Embodiment 1:
Take by weighing dry tobacco leaf that 1g crushes in the 50mL Erlenmeyer flask, add ETHYLE ACETATE 10mL, first supersound extraction 1min.With above-mentioned solution left standstill 1h, filtration, utilize the comprehensive two dimensional gas chromatography flight time mass spectrum to analyze.Identify alcohols material 20 kinds of (comprising phenols), 53 kinds of aldoketoneses, 5 kinds of ester classes altogether, total relative extracted amount is 247.66, and said relative extracted amount is meant the peak area and the interior mark peak area ratio of assay.Analytical results is seen table 1.
Embodiment 2:
Take by weighing dry tobacco leaf that 1g crushes in the 50mL Erlenmeyer flask, add ETHYLE ACETATE 10mL, first supersound extraction 5min.With above-mentioned solution left standstill 3h, filtration, utilize the comprehensive two dimensional gas chromatography flight time mass spectrum to analyze.Identify alcohols material 22 kinds of (comprising phenols), 60 kinds of aldoketoneses, 5 kinds of ester classes altogether, total relative extracted amount is 266.87.Analytical results is seen table 1.
Embodiment 3:
Take by weighing dry tobacco leaf that 1g crushes in the 50mL Erlenmeyer flask, add ETHYLE ACETATE 10mL, first supersound extraction 30min.With above-mentioned solution left standstill 24h, filtration, utilize the comprehensive two dimensional gas chromatography flight time mass spectrum to analyze.Identify alcohols material 21 kinds of (comprising phenols), 56 kinds of aldoketoneses, 5 kinds of ester classes altogether, total relative extracted amount is 260.37.Analytical results is seen table 1.
The neutral flavor composition that table 1 extracts
Embodiment 4:
Take by weighing dry tobacco leaf that 1g crushes in the 50mL Erlenmeyer flask, add ETHYLE ACETATE 5mL, first supersound extraction 5min.With above-mentioned solution left standstill 3h, filtration, utilize the comprehensive two dimensional gas chromatography flight time mass spectrum to analyze.Identify alcohols material 22 kinds of (comprising phenols), 55 kinds of aldoketoneses, 5 kinds of ester classes altogether, total relative extracted amount is 264.84.
Embodiment 5:
Take by weighing dry tobacco leaf that 1g crushes in the 50mL Erlenmeyer flask, add ETHYLE ACETATE 25mL, first supersound extraction 5min.With above-mentioned solution left standstill 3h, filtration, utilize the comprehensive two dimensional gas chromatography flight time mass spectrum to analyze.Identify alcohols material 22 kinds of (comprising phenols), 59 kinds of aldoketoneses, 5 kinds of ester classes altogether, total relative extracted amount is 266.76.
Embodiment 6:
Flavour ingredient to the tobacco leaf after embodiment 2 extraction carries out second extraction, and compares with flavour ingredient kind that embodiment 2 extracts gained for the first time, and comparative result is seen table 2.Can find out that from table 2 process for extracting of the present invention is higher to tobacco leaf neutral flavor composition extraction efficiency, second extraction does not analyze corresponding neutral flavor composition.
The comparison of table 2 extraction time
Only be preferred implementation of the present invention below, should be pointed out that above-mentioned preferred implementation should not be regarded as limitation of the present invention, protection scope of the present invention should be as the criterion with claim institute restricted portion.For those skilled in the art, do not breaking away from the spirit and scope of the present invention, can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (7)
1. the process for extracting of a tobacco leaf neutral flavor composition is characterized in that, is raw material with the tobacco leaf; ETHYLE ACETATE is for extracting solvent, and at room temperature supersound process leaves standstill for some time again; Remove by filter offal, obtain the neutral flavor composition of alcohol, aldehyde, ketone, ester compound.
2. the process for extracting of a kind of tobacco leaf neutral flavor composition according to claim 1 is characterized in that, said every gram tobacco leaf adapted ETHYLE ACETATE 5～25mL.
3. the process for extracting of a kind of tobacco leaf neutral flavor composition according to claim 2 is characterized in that, said every gram tobacco leaf adapted ETHYLE ACETATE 10mL.
4. the process for extracting of a kind of tobacco leaf neutral flavor composition according to claim 1 is characterized in that, the said supersound process time is lower than 30min.
5. the process for extracting of a kind of tobacco leaf neutral flavor composition according to claim 4 is characterized in that, the said supersound process time is 5min.
6. the process for extracting of a kind of tobacco leaf neutral flavor composition according to claim 1 is characterized in that said time of repose is lower than 24h.
7. the process for extracting of a kind of tobacco leaf neutral flavor composition according to claim 6 is characterized in that, said time of repose is 3h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101977664A CN102703218A (en) | 2012-06-15 | 2012-06-15 | Method for extracting neutral fragrance ingredients from tobacco leaves |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012101977664A CN102703218A (en) | 2012-06-15 | 2012-06-15 | Method for extracting neutral fragrance ingredients from tobacco leaves |
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| CN102703218A true CN102703218A (en) | 2012-10-03 |
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| CN2012101977664A Pending CN102703218A (en) | 2012-06-15 | 2012-06-15 | Method for extracting neutral fragrance ingredients from tobacco leaves |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109061015A (en) * | 2018-10-30 | 2018-12-21 | 福建中烟工业有限责任公司 | Detect method, kit and its application of γ-orcylaldehyde class compound in tobacco product and/or essence spice for cigarette |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1234403A (en) * | 1999-04-23 | 1999-11-10 | 西北农业大学无公害农药研究服务中心 | Nicotine extraction process |
| CN102161935A (en) * | 2010-12-31 | 2011-08-24 | 广东中烟工业有限责任公司 | Endogenous perfume from tobacco as well as preparation method and application thereof |
| CN102178346A (en) * | 2011-05-30 | 2011-09-14 | 昌宁德康生物科技有限公司深圳分公司 | Production method for extracting absolute oil of tobacco leaves |
-
2012
- 2012-06-15 CN CN2012101977664A patent/CN102703218A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1234403A (en) * | 1999-04-23 | 1999-11-10 | 西北农业大学无公害农药研究服务中心 | Nicotine extraction process |
| CN102161935A (en) * | 2010-12-31 | 2011-08-24 | 广东中烟工业有限责任公司 | Endogenous perfume from tobacco as well as preparation method and application thereof |
| CN102178346A (en) * | 2011-05-30 | 2011-09-14 | 昌宁德康生物科技有限公司深圳分公司 | Production method for extracting absolute oil of tobacco leaves |
Non-Patent Citations (1)
| Title |
|---|
| 张泽生等: "超声波辅助提取烟叶中的茄尼醇", 《食品与生物技术学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109061015A (en) * | 2018-10-30 | 2018-12-21 | 福建中烟工业有限责任公司 | Detect method, kit and its application of γ-orcylaldehyde class compound in tobacco product and/or essence spice for cigarette |
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Application publication date: 20121003 |