CN102696539A - Air transportation method of laboratory animals between plain and plateau - Google Patents
Air transportation method of laboratory animals between plain and plateau Download PDFInfo
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- CN102696539A CN102696539A CN2012101600899A CN201210160089A CN102696539A CN 102696539 A CN102696539 A CN 102696539A CN 2012101600899 A CN2012101600899 A CN 2012101600899A CN 201210160089 A CN201210160089 A CN 201210160089A CN 102696539 A CN102696539 A CN 102696539A
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Abstract
The invention relates to an air transportation method of laboratory animals between plain and plateau. The method comprises the following steps: (1) purchasing and early feeding animals: purchasing laboratory animals from the plain, feeding the animals in a clean-level environment by using standard feed and sterilized drinking water for 3-7 days; (2) conducting quarantine inspection of animals; (3) delivering animals: feeding the laboratory animals in a breathable iron cage of which bottom part is provided with a tray, wherein the iron cage is provided with the complete feed and the drinking water; and then feeding laboratory animals in an aviation logistics company before the aviation transportation; (4) conducting aviation transportation on animals: before the fright departure, removing the drinking water, reserving the complete feed, packing all the laboratory animals in a regular wooden box, placing the regular wooden box in an aerobic cabin of the day scheduled flight, and transporting the regular wooden box to the destination; and (5) receiving animals: after the flight arrival, immediately opening the box to transfer the laboratory animals to a cage with padding materials; offering the complete feed and the drinking water, and then transporting to a lab. The air transportation method has the characteristics of fast speed, safety, economy and simple operation.
Description
Technical field
The present invention relates to the research of laboratory animal environmental ecology in the experimental zoology research field, relate in particular to the experiment small animal air transportation method between a kind of Plain and plateau.
Background technology
In study of high altitude medicine, the bodily forms such as mouse, rat, pika are of moderate size, and the behavior variation is widely used in researchs such as altitude physiology, pathology, pharmacodynamics, pharmacology, pharmacokinetics.Carry out zoopery in the highlands; The laboratory animal of highlands has the advantages that to adapt to altitude environment, and (the highlands laboratory animal is because natural selection; Adapted to anoxic environment, be not suitable for doing anoxia model, and the hypoxemia adaptive capacity has inheritance); Many experiments need be transported animal from the low altitude area; As carry out the plateau zoopery in the low altitude area, though the low-pressure oxygen cabin of simulated high altitude has characteristics such as plateau low pressure, hypoxemia, and also there are many difference in altitude environment on the spot; Above-mentioned either way have from the Plain transportation radical plateau of animal or from the needs in radical Plain, plateau, therefore set up a kind of fast, safety, economy, Plain and the experiment small animal air transportation method between the plateau simple to operate be very necessary.
Summary of the invention
Technical problem to be solved by this invention provide a kind of fast, safety, economy, Plain simple to operate and the experiment small animal air transportation method between the plateau.
For addressing the above problem, the experiment small animal air transportation method between a kind of Plain of the present invention and plateau may further comprise the steps:
⑴ purchase of animal and early stage raise: buy laboratory animal from the flat area, adopt standard feed and sterilising drinking water under cleaning level environment, to feed 3 ~ 7 days;
⑵ the quarantine of animal: said laboratory animal is all quarantined starting shipment the local county/district above animal quarantine of level mechanism, run quarantine inspection well;
⑶ shipping of animal: said experimental animal feeding has in the irony cage tool of pallet in ventilative bottom; In this irony cage tool the complete feed of 20 ~ 150g and the drinking water of 50 ~ 200ml are arranged; Then aviation start shipment preceding 5 ~ 8 hours by temperature be 18 ~ 25 ℃ band air-conditioning Freight Transport to the aviation logistics company, and raise in the aviation logistics company; Raise 5 ~ 20 of said laboratory animal in said each irony cage tool;
⑷ the air transportation of animal: the flight takeoff reach removes said drinking water; Keep said complete feed; All said laboratory animal cage tools are packed in regular wooden case, and place flight aerobic cabin on the same day, airflight was transported to the destination after 1 ~ 3 hour then---the highlands; In said each regular wooden case 5 ~ 200 of said laboratory animal are arranged;
⑸ accessing of animal: access the people and before said flight arrives, arrive purpose; Unpacking immediately after said flight arrives is transferred to said laboratory animal in the band bedding and padding cage tool, gives complete feed and drinking water, is that 18 ~ 25 ℃ band air-conditioning Freight Transport is to the laboratory by temperature then.
Laboratory animal among the said step ⑴ is meant that body weight is mouse, rat and/or the pika of 10 ~ 1500g.
Flat area height above sea level among the said step ⑴ is 0 ~ 500 meter.
Irony cage tool among the said step ⑶ is meant the cage tool of the 25 cm ~ 35cm * 40 cm ~ 50cm * 25 cm ~ 35cm of the fence formation that comprises gap≤1.5cm; The front of this cage tool is provided with the up and down wicket of 10 cm ~ 20cm * 10 cm ~ 20cm, and it highly is the plastic pallet of 2 ~ 5cm that its bottom is provided with.
Regular wooden case among the said step ⑷ is meant that thickness is the casing of 2 ~ 10mm, internal volume≤9 * 25 cm ~ 35cm * 40 cm ~ 50cm * 25 cm ~ 35cm, the air-vent that it is 0.5 ~ 5cm that this four sides, casing front, rear, left and right is evenly equipped with 6 ~ 120 diameters respectively.。
Highlands height above sea level among the said step ⑷ is 1500 ~ 5000m.
The said flat area and two places, the said highlands temperature difference are 15 ~ 35 ℃.
The present invention compared with prior art has the following advantages:
1, because the present invention adopts the mode of aerobic cabin air transportation to carry out the transportation of laboratory animal in the experiment of plateau, therefore, quick, safety.
2, because the present invention adopts iron cage tool and wooden case transportation, and material is easy to get, the nail system is convenient, therefore, not only simple to operate and economical.
Embodiment
Experiment small animal air transportation method between a kind of Plain and plateau may further comprise the steps:
⑴ purchase of animal and early stage raise: from height above sea level is that 0 ~ 500 meter flat area buys that laboratory animal---body weight is mouse, rat and/or the pika of 10 ~ 1500g, adopts the nursing 3 ~ 7 days under cleaning level environment of standard feed and sterilising drinking water.
⑵ the quarantine of animal: laboratory animal is all quarantined starting shipment the local county/district above animal quarantine of level mechanism, assurance does not have the epidemic situation of forbidding air transportation, runs quarantine inspection well.
⑶ shipping of animal: experimental animal feeding has in the irony cage tool of pallet in ventilative bottom; In this irony cage tool the complete feed of 20 ~ 150g and the drinking water of 50 ~ 200ml are arranged; Then aviation start shipment preceding 5 ~ 8 hours by temperature be 18 ~ 25 ℃ band air-conditioning Freight Transport to the aviation logistics company, and raise in the aviation logistics company; Breed experimental animals 5 ~ 20 in each irony cage tool.
Wherein: irony cage tool is meant the cage tool of the 25 cm ~ 35cm * 40 cm ~ 50cm * 25 cm ~ 35cm of the fence formation that comprises gap≤1.5cm; The front of this cage tool is provided with the up and down wicket of 10 cm ~ 20cm * 10 cm ~ 20cm, and it highly is the plastic pallet of 2 ~ 5cm that its bottom is provided with.
⑷ the air transportation of animal: the flight takeoff reach removes drinking water; Keep complete feed; All laboratory animal cage tools are packed in regular wooden case, and place flight aerobic cabin on the same day, airflight was transported to the destination after 1 ~ 3 hour then---and height above sea level is the highlands of 1500 ~ 5000m; 5 ~ 200 of laboratory animal are arranged in each regular wooden case.
Wherein: regular wooden case is meant that thickness is the casing of 2 ~ 10mm, internal volume≤9 * 25 cm ~ 35cm * 40 cm ~ 50cm * 25 cm ~ 35cm, the air-vent that it is 0.5 ~ 5cm that this four sides, casing front, rear, left and right is evenly equipped with 6 ~ 120 diameters respectively.
⑸ accessing of animal: access the people and before flight arrives, arrive purpose; Unpacking immediately after flight arrives is transferred to laboratory animal in the band bedding and padding cage tool, gives complete feed and drinking water, is that 18 ~ 25 ℃ band air-conditioning Freight Transport is to the laboratory by temperature then.
Wherein: the flat area and two places, the highlands temperature difference are 15 ~ 35 ℃.
Adopt the reason of band bedding and padding cage tool to be: 1, flight is a constant temperature, and it is unstable to arrive the back temperature, and bedding and padding can be incubated; 2, the cage tool of air transportation is different with normal cage for rearing poultry, and normal cage tool is fence except lid, and the bottom is the opaque resin material around reaching, and after the air transportation packing, the cage tool stacks easy anoxic; 3, the feeding environment of laboratory animal need be with bedding and padding under the normal condition, and a period of time also need be changed bedding and padding, owing to meeting in bedding and padding deficiency on the flight and the transportation is contaminated, so needs to change the cage tool after the flight arrival.
Embodiment60 Wistar male rats are transported to the Lanzhou, Gansu from Shanghai, comprise following steps:
⑴ purchase of rat and early stage raise: buy male Wistar rat from Shanghai Slac Experimental Animal Co., Ltd.; Average weight is 180 ± 20g, raises pharmaceutical college of the The 2nd Army Medical College zoopery center in Shanghai City, and local height above sea level is 55 meters; Temperature is 24 ℃; Humidity is 70%, and standard complete feed and sterilising drinking water are fed, and cleaning level environment was fed 5 days down.
⑵ the quarantine of rat: all rats are quarantined no epidemic situation in the supervision of Yangpu District animal health.
⑶ shipping of rat: the rat flight takeoff was shipped the aviation logistics company in east in preceding 6 hours, and every cage rat is given has feed 60g, sterilising drinking water 120ml; Per 15 rat feedings have in the irony cage tool of pallet in ventilative bottom, totally 4 cages, and cage tool specification is 30cm * 45cm * 30cm; Cage tool fence gap is 0.6cm, has wicket movable up and down, and specification is 12cm * 12cm; Can supply the small animal turnover; Plastic pallet height 3cm, band air-conditioning lorry temperature is 23 ℃, is transported to the aviation logistics company and raises in logistics company.
⑷ the air transportation of rat: the flight takeoff reach removes drinking water, keeps complete feed, and all big mouse cage tools are packed in regular wooden case; Wooden case thickness 5mm; Specification is 60cm * 90cm * 60cm, and wooden case all around four sides respectively leaves air-vent, and each air-vent diameter is 2.5cm; Every quantity is 36,60 of transportation rats.Afternoon on the same day, 3:55 flight aerobic cabin was transported to Zhong Chuan airport, Lanzhou from the ZSPD.
⑸ accessing of rat: the 19:00 flight arrives Lanzhou, and 20:00 unpacks rat is transferred in the band bedding and padding cage tool, gives complete feed and drinking water; Air-conditioning lorry temperature is 22 ℃, and 20:38 therefrom sets out on the airport, river, and 21:40 is transported to the laboratory; 3817 meters of height above sea levels, 6 ℃ of temperature.
Claims (7)
1. the experiment small animal air transportation method between Plain and plateau may further comprise the steps:
⑴ purchase of animal and early stage raise: buy laboratory animal from the flat area, adopt standard feed and sterilising drinking water under cleaning level environment, to feed 3 ~ 7 days;
⑵ the quarantine of animal: said laboratory animal is all quarantined starting shipment the local county/district above animal quarantine of level mechanism, run quarantine inspection well;
⑶ shipping of animal: said experimental animal feeding has in the irony cage tool of pallet in ventilative bottom; In this irony cage tool the complete feed of 20 ~ 150g and the drinking water of 50 ~ 200ml are arranged; Then aviation start shipment preceding 5 ~ 8 hours by temperature be 18 ~ 25 ℃ band air-conditioning Freight Transport to the aviation logistics company, and raise in the aviation logistics company; Raise 5 ~ 20 of said laboratory animal in said each irony cage tool;
⑷ the air transportation of animal: the flight takeoff reach removes said drinking water; Keep said complete feed; All said laboratory animal cage tools are packed in regular wooden case, and place flight aerobic cabin on the same day, airflight was transported to the destination after 1 ~ 3 hour then---the highlands; In said each regular wooden case 5 ~ 200 of said laboratory animal are arranged;
⑸ accessing of animal: access the people and before said flight arrives, arrive purpose; Unpacking immediately after said flight arrives is transferred to said laboratory animal in the band bedding and padding cage tool, gives complete feed and drinking water, is that 18 ~ 25 ℃ band air-conditioning Freight Transport is to the laboratory by temperature then.
2. the experiment small animal air transportation method between a kind of Plain as claimed in claim 1 and plateau is characterized in that: the laboratory animal among the said step ⑴ is meant that body weight is mouse, rat and/or the pika of 10 ~ 1500g.
3. the experiment small animal air transportation method between a kind of Plain as claimed in claim 1 and plateau, it is characterized in that: the flat area height above sea level among the said step ⑴ is 0 ~ 500 meter.
4. the experiment small animal air transportation method between a kind of Plain as claimed in claim 1 and plateau; It is characterized in that: the irony cage tool among the said step ⑶ is meant the cage tool of the 25 cm ~ 35cm * 40 cm ~ 50cm * 25 cm ~ 35cm of the fence formation that comprises gap≤1.5cm; The front of this cage tool is provided with the up and down wicket of 10 cm ~ 20cm * 10 cm ~ 20cm, and it highly is the plastic pallet of 2 ~ 5cm that its bottom is provided with.
5. the experiment small animal air transportation method between a kind of Plain as claimed in claim 1 and plateau; It is characterized in that: the regular wooden case among the said step ⑷ is meant that thickness is the casing of 2 ~ 10mm, internal volume≤9 * 25 cm ~ 35cm * 40 cm ~ 50cm * 25 cm ~ 35cm, the air-vent that it is 0.5 ~ 5cm that this four sides, casing front, rear, left and right is evenly equipped with 6 ~ 120 diameters respectively.
6. the experiment small animal air transportation method between a kind of Plain as claimed in claim 1 and plateau, it is characterized in that: the highlands height above sea level among the said step ⑷ is 1500 ~ 5000m.
7. the experiment small animal air transportation method between a kind of Plain as claimed in claim 1 and plateau is characterized in that: the said flat area and two places, the said highlands temperature difference are 15 ~ 35 ℃.
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| CN2012101600899A CN102696539A (en) | 2012-05-22 | 2012-05-22 | Air transportation method of laboratory animals between plain and plateau |
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| CN2012101600899A CN102696539A (en) | 2012-05-22 | 2012-05-22 | Air transportation method of laboratory animals between plain and plateau |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113907041A (en) * | 2021-12-13 | 2022-01-11 | 天津医科大学总医院空港医院 | Method for establishing acute plateau low-pressure hypoxia lung injury mouse model |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201371987Y (en) * | 2009-03-13 | 2009-12-30 | 程实 | Foldable type live poultry and livestock transporting box |
| CN201657680U (en) * | 2010-04-23 | 2010-12-01 | 浙江海洋学院 | Mouse cage |
-
2012
- 2012-05-22 CN CN2012101600899A patent/CN102696539A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201371987Y (en) * | 2009-03-13 | 2009-12-30 | 程实 | Foldable type live poultry and livestock transporting box |
| CN201657680U (en) * | 2010-04-23 | 2010-12-01 | 浙江海洋学院 | Mouse cage |
Non-Patent Citations (6)
| Title |
|---|
| 《中华人民共和国国家标准》 20110616 中华人民共和国国家质量监督检验检疫总局 活体动物航空运输包装通用要求 第2-3页以及38-39页 1-7 , * |
| 《中华人民共和国国家标准》 20111230 中华人民共和国国家质量监督检验检疫总局等 活体动物航空运输载运GB/T27882-2011 第2页及第4-5页 1-7 , * |
| 中华人民共和国国家质量监督检验检疫总局: "活体动物航空运输包装通用要求", 《中华人民共和国国家标准》 * |
| 中华人民共和国国家质量监督检验检疫总局等: "活体动物航空运输载运GB/T27882-2011", 《中华人民共和国国家标准》 * |
| 李德濮: "动物运输保健八要", 《畜牧与饲料科学》 * |
| 王珑等: "实验动物的运输", 《中国实验动物学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113907041A (en) * | 2021-12-13 | 2022-01-11 | 天津医科大学总医院空港医院 | Method for establishing acute plateau low-pressure hypoxia lung injury mouse model |
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Application publication date: 20121003 |