CN102675448A - Method for isolating casein components in milk - Google Patents
Method for isolating casein components in milk Download PDFInfo
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- CN102675448A CN102675448A CN2012101873928A CN201210187392A CN102675448A CN 102675448 A CN102675448 A CN 102675448A CN 2012101873928 A CN2012101873928 A CN 2012101873928A CN 201210187392 A CN201210187392 A CN 201210187392A CN 102675448 A CN102675448 A CN 102675448A
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Abstract
A method for isolating casein components in milk includes that: buffalo milk is used as the raw material, and isoelectric point slow acid precipitation and grading calcium precipitation are combined to isolate alpha s-casein, beta-casein and k-casein in buffalo milk. Gross recovery of all casein components after grading calcium precipitation is 88%, wherein mass percent of the isolated alpha s-casein and the isolated beta-casein respectively reaches 91.6% and 67.8%, mass percent of the isolated k-casein improves from 13.8% to 43.3%, and the basis for further purification is provided. Simultaneously, mass percent of the beta-casein can be improved to be 100% by adopting the technical route of secondary grading calcium precipitation theoretically. The method is simple to operate, obvious in isolating effect and suitable for industrialized production.
Description
Technical field
The present invention relates to a kind of method of separating Caseinum componemt in the buffalo milk, especially a kind of simple process of passing through is simultaneously with α s-casein, beta-casein and the isolating method of κ-casein.
Background technology
Milk-protein mainly is made up of casein and whey-protein in the buffalo milk, and is wherein high on behalf of example to hybridize, α
S1-casein, α
S2It is caseic 35.30%, 16.97%, 33.88%, 13.85% that-casein, beta-casein, κ-casein account for respectively, and alpha-lactalbumin, beta-lact oglobulin account for 30.95%, 48.49% of whey-protein respectively.Casein exists with the complex body form that casein micelles combines calcium phosphate to form, and is called " calcium caseinate-calcium phosphate complex body " (Ca-phosphocaseinate or Ca-caseinate-phosphhate-complate).
In recent decades; In order to study the stabiliser of casein micelles system; And the structure function characteristic of each Caseinum componemt; Therefore the separation and purification Caseinum componemt is the basis of carrying out above-mentioned research, and the success or failure of the efficient of separation and purification and the follow-up multinomial work of quality direct relation become the focus of research.The separation method of Caseinum componemt mainly comprises precipitate and separate, chromatographic separation, membrane sepn and enzyme process separation etc. at present; Wherein, Chromatographic separation is mainly used in laboratory scale, and membrane sepn and enzyme process separation generally all need combine additive method to accomplish separating step jointly, and using is precipitate and separate the most widely; Mainly be to utilize each Caseinum componemt in differing temps, ionic strength; Deliquescent difference is separated in the solution of calcium concn, the yield and the purity of the main object product of sepn process, but less to the systematic study of its sepn process.
Summary of the invention
The purpose of this invention is to provide a kind of method of separating Caseinum componemt in the buffalo milk, can separate α s-casein, beta-casein and κ-casein simultaneously, and simple to operate, and separating effect is obvious, suitability for industrialized production.
The object of the invention is realized through following technical scheme: a kind of method of separating Caseinum componemt in the buffalo milk may further comprise the steps:
1. fresh water milk; Behind 8000g, 4 ℃ of following centrifugal degreasing 20～30min, adopt the slowly heavy method of acid of iso-electric point, slowly regulate pH to 4.5 ~ 4.7 of milk after the degreasing with acid solution; Keep deposition in 8000g, 4 ℃ of down centrifugal 20～30min; Distilled water wash 2 times promptly obtains rough casein powder, and freeze-drying is subsequent use;
2. slowly acid is heavy with iso-electric point, and the casein powder that obtains after the freeze-drying is mixed with 2% casein solution, adopts alkaline solution slowly to regulate pH to 7.0 ~ 7.5 of casein solution;
3. according to the Ca of 0.06mol
2+2. concentration add the divalence soluble calcium salt in the casein solution in step, and centrifugal 20～30min under 8000g keeps supernatant and deposition, and deposition is dissolved in the disodium ethylene diamine tetra-acetic acid solution, and dialysis back freeze-drying promptly obtains massfraction and reaches 91.6% α
s-casein;
4. 3. continue to add the divalence soluble calcium salt in the supernatant of centrifugal reservation in step, make Ca
2+The concentration final concentration arrives 0.3mol; Recentrifuge keeps supernatant and deposition, obtains massfraction after the supernatant dialysis freeze-drying and reaches κ-casein of 43.3%; Deposition is dissolved in the disodium ethylene diamine tetra-acetic acid solution, and dialysis back freeze-drying promptly obtains massfraction and reaches 100% beta-casein.
Said casein is the casein of buffalo milk and buffalo milk goods.
Said buffalo milk is that the purebred buffalo milk of mora, mora first-filial generation buffalo milk, mora hybridization height are for buffalo milk; Said buffalo milk goods are buffalo milk yogurt, buffalo milk cheese, buffalo milk milk powder.
Said divalence soluble calcium salt is a calcium chloride.
Said disodium ethylene diamine tetra-acetic acid solution is that EDTA Disodium is mixed with 0.06～0.3mol solution.
Said acid solution is that glacial acetic acid or hydrochloric acid are mixed with 0.05～0.1mol solution.
Said alkaline solution is that sodium hydroxide or ammoniacal liquor are mixed with 0.1～0.2mol solution.
The present invention has the following advantages:
1. the present invention adopts that classification calcium is heavy to have carried out separated in synchronization to α s-casein, beta-casein and three kinds of Caseinum componemts of κ-casein; Operating procedure is simple; A kind of method of simple possible is provided for fairly large separation Caseinum componemt; For the prepared in laboratory sample reference is provided on the one hand, preparation also has the certain experiences meaning to heavy industrialization simultaneously, for follow-up industrial application is provided convenience.
2. the α s-casein, beta-casein and the κ that produce of the present invention-casein purity is high, and for the production of reagent type α s-casein, beta-casein provides method, the reagent of this process using has seldom been practiced thrift preparation cost simultaneously.
3. α s-casein, beta-casein and κ-casein yield of producing of the present invention is all higher, can efficent use of resources.
Description of drawings
The casein electrophorogram that Fig. 1 obtains after sinking for iso-electric point is slowly sour.
The slowly sour heavy casein that obtains of whey-protein 4-iso-electric point behind the casein is removed in the slow acid of buffalo milk whole protein 3-iso-electric point after the 1-LMWP mark article 2-degreasing, and last appearance concentration all is diluted to 1000 μ g/mL, applied sample amount 10 μ L.
The casein electrophorogram that Fig. 2 obtains after heavy for classification calcium.
1-LMWP mark article, 2-, 3-, 4-, 5-, 6-, 7-are respectively 0.30mol, 0.25mol, 0.20mol, 0.15mol, 0.10mol, 0.06molCa
2+Handle casein postprecipitation lysate, 8-, 9-, 10-, 11-, 12-, 13-are respectively 0.30mol, 0.25mol, 0.20mol, 0.15mol, 0.10mol, 0.06mol Ca
2+Supernatant behind the processing casein, last appearance concentration all is diluted to 1000 μ g/mL, applied sample amount 10 μ L.
The protein contnt typical curve of Fig. 3 for adopting the Xylene Brilliant Cyanine G method to obtain.
Embodiment
Through experiment the separating effect of Caseinum componemt is estimated below.
1, the slowly sour heavy rough segmentation casein Evaluation on effect of iso-electric point
In order to estimate the slowly caseic purity in the heavy back of acid of iso-electric point, the albumen after we adopt SDS-PAGE to degreasing in the cow's milk form and iso-electric point slowly the albumen in the heavy postprecipitation of acid and the supernatant form and carried out comparative analysis.The result sees Fig. 1.
Can know by Fig. 1; The whole protein of swimming lane 2 is after the slow acid of iso-electric point is heavy among Fig. 1; Basically can bovine serum albumin in the buffalo milk and whey-protein all be removed; Obtain thick casein like swimming lane among Fig. 14, and impurity such as bovine serum albumin and whey-protein stay yogurt behind the heavy casein of acid like swimming lane among Fig. 13 clear in.This method is simple to operate, good separation, suitability for scale production.
2, the heavy Caseinum componemt Evaluation on effect of classification calcium
In order to estimate the separation case of Caseinum componemt under the various calcium concentrations, we adopt SDS-PAGE and Xylene Brilliant Cyanine G method to 0.06mol, 0.10mol, 0.15mol, 0.20mol, 0.25mol, 0.30molCa
2+Caseinum componemt after the processing has carried out purity and yield analysis.The result sees Fig. 2, Fig. 3 and table 1.
As can be seen from Figure 2, Ca
2+When concentration reaches 0.06mol, the α in the casein
s-casein precipitates fully, and has beta-casein also to precipitate simultaneously, and in the throw out except above two kinds of Caseinum componemts, bovine serum albumin also takes place to precipitate fully.Simultaneously, at 0.06mol-0.30mol Ca
2+Concentration is handled in the electrophoretic band of casein resolution of precipitate liquid, 0.06molCa
2+Faint κ-casein band appears in concentration, along with Ca
2+Concentration continues to increase, and κ-casein band constantly weakens on the contrary, especially works as Ca
2+When concentration reached 0.20mol, κ-casein band almost disappeared.At 0.06mol-0.30molCa
2+Concentration is handled in the caseic supernatant, mainly contains beta-casein and κ-casein two bands, and along with Ca
2+The increase gradually of concentration, the beta-casein band weakens gradually, and is consistent with protein contnt variation tendency in the Xylene Brilliant Cyanine G mensuration supernatant.According to above phenomenon, can judge, through Ca
2+The precedence of three kinds of components of deposition casein is: α s-casein calcium at first is heavy, secondly is beta-casein, is κ-casein at last.Can find out also that by Fig. 2 κ-casein is at extremely low 0.06mol Ca
2+Under the concentration existence condition, begin to precipitate, with Ca
2+The continuous increase of concentration, κ-casein dissolves on the contrary, and redeposition is not separated out.This and α
s-casein is met calcium deposit, causes that κ-caseic crosslinked deposition is relevant.At Ca
2+All do not contain bovine serum albumin in all bands of casein supernatant after handling, and all contain band very weak beta-lact oglobulin, ALA, explain that bovine serum albumin is to Ca
2+Very responsive, very low concentration promptly precipitates, and beta-lact oglobulin and ALA are to Ca
2+Certain tolerance is but arranged.
Caseic quality in the heavy component of each grade calcium of table 1
According to table 1, can obviously find out, at 0.06mol-0.30mol Ca
2+Concentration is handled in the caseic supernatant; Protein quality gradually reduces, and in conjunction with electrophoretogram, main ingredient is beta-casein and κ-casein in this process supernatant; And κ-casein amount of separating out is little in the deposition, explains that the precipitin reaction of beta-casein mainly takes place this process.A kind of method of simple separation beta-casein can be proposed in view of the above---secondary calcium sinks method: promptly adopt 0.06mol Ca earlier
2+Concentration is handled casein, and the centrifugal deposition of abandoning promptly discards α
s-casein keeps supernatant, adds the divalence soluble calcium salt, makes Ca
2+Final concentration reaches 0.3mol, and recentrifuge keeps deposition, and deposition is dissolved in the disodium ethylene diamine tetra-acetic acid solution, stirs it is fully dissolved, and can obtain massfraction after the dialysis and reach 100% beta-casein.
After further estimating each calcium concentration processing casein, α s-casein, beta-casein and the κ-caseic massfraction in each Caseinum componemt, we adopt following method of calculation that it is estimated:
If ignore bovine serum albumin and the whey-protein in κ-casein and the supernatant in the deposition, according to α
s-casein, beta-casein and the κ-casein ratio 3.77:2.45:1 in buffalo milk; In conjunction with the heavy casein total yield of classification calcium; Can estimate various Caseinum componemt massfraction ratios in deposition and the supernatant, be example with the caseic massfraction of α s-in the deposition:
A
1=B
1×B
2×B
3
In the formula: A
1Be α s-casein quality in the deposition; B
1Be the casein total mass; B
2Be the casein total yield; B
3Be the shared massfraction of α s-casein in the casein in theory.
In the formula: A is α s-casein quality mark in the deposition; A
1Be α s-casein quality in the deposition; A
2Be the deposition quality.
Calculation result is seen table 2:
α in the heavy component of each grade calcium of table 2
s-casein, beta-casein and κ-casein quality mark
According to table 2, at 0.06mol-0.30mol Ca
2+In the concentration range, α in the deposition
s-caseic massfraction reduces to 76.8% by 91.6%, and the massfraction of beta-casein reduces to 56.7% by 67.8% in the supernatant, and κ-caseic massfraction is promoted to 43.3% by 32.2%.
Get the purebred buffalo milk of 1.00kg mora, behind 8000g, 4 ℃ of following centrifugal degreasing 30min, adopt the 0.1mol glacial acetic acid slowly to regulate milk pH to 4.5 ~ 4.7 after the degreasing, keep deposition behind the centrifugal 20min down in 8000g, 4 ℃, freeze-drying promptly obtains thick casein lyophilized powder.The casein sample ligand processed 2% protein solution; Slowly regulate pH to 7.0 ~ 7.5 of casein solution with the 0.1mol sodium hydroxide solution; Ratio according to 0.06mol adds the divalence soluble calcium salt; Centrifugal reservation supernatant and deposition are dissolved in deposition in the 0.06mol disodium ethylene diamine tetra-acetic acid solution, and dialysis back freeze-drying promptly obtains massfraction and reaches 91.6% α s-casein; In supernatant, continue to add the divalence soluble calcium salt, make its concentration reach 0.3mol, recentrifuge keeps supernatant and deposition.Deposition is dissolved in the 0.30mol disodium ethylene diamine tetra-acetic acid solution, and dialysis back freeze-drying promptly obtains massfraction and reaches 100% beta-casein; The supernatant dialysis back freeze-drying that keeps promptly obtains massfraction and reaches κ-casein of 43.3%;
Get 1.00kg mora first-filial generation buffalo milk; Behind 8000g, 4 ℃ of following centrifugal degreasing 30min; Milk pH to 4.5 ~ 4.7 after the employing 0.05mol salt slow acid adjusting degreasing keep deposition in 8000g, 4 ℃ of down centrifugal 30min, and freeze-drying promptly obtains thick casein lyophilized powder.The casein sample ligand processed 2% protein solution; Slowly regulate pH to 7.0 ~ 7.5 of casein solution with the 0.1mol sodium hydroxide solution; Ratio according to 0.06mol adds the divalence soluble calcium salt; Centrifugal reservation supernatant and deposition are dissolved in deposition in the 0.06mol disodium ethylene diamine tetra-acetic acid solution, and dialysis back freeze-drying promptly obtains massfraction and reaches 91.6% α s-casein; In supernatant, continue to add the divalence soluble calcium salt, make its concentration reach 0.3mol, recentrifuge keeps supernatant and deposition.Deposition is dissolved in the 0.25mol disodium ethylene diamine tetra-acetic acid solution, and dialysis back freeze-drying promptly obtains massfraction and reaches 100% beta-casein; The supernatant dialysis back freeze-drying that keeps promptly obtains massfraction and reaches κ-casein of 43.3%;
It is high for buffalo milk to get the hybridization of 1.00kg mora; Behind 8000g, 4 ℃ of following centrifugal degreasing 20min; Adopt the 0.1mol glacial acetic acid slowly to regulate milk pH to 4.5 ~ 4.7 after the degreasing, keep deposition in 8000g, 4 ℃ of down centrifugal 30min, freeze-drying promptly obtains thick casein lyophilized powder.The casein sample ligand processed 2% protein solution; Slowly regulate pH to 7.0 ~ 7.5 of casein solution with the 0.2mol ammonia soln; Ratio according to 0.06mol adds the divalence soluble calcium salt; Centrifugal reservation supernatant and deposition are dissolved in deposition in the 0.06mol disodium ethylene diamine tetra-acetic acid solution, and dialysis back freeze-drying promptly obtains massfraction and reaches 91.6% α s-casein; In supernatant, continue to add the divalence soluble calcium salt, make its concentration reach 0.3mol, recentrifuge keeps supernatant and deposition.Deposition is dissolved in the 0.20mol disodium ethylene diamine tetra-acetic acid solution, and dialysis back freeze-drying promptly obtains massfraction and reaches 100% beta-casein; The supernatant dialysis back freeze-drying that keeps promptly obtains massfraction and reaches κ-casein of 43.3%;
Claims (7)
1. a method of separating Caseinum componemt in the buffalo milk is characterized in that, this method may further comprise the steps:
1. fresh water milk; Behind 8000g, 4 ℃ of following centrifugal degreasing 20～30min, adopt the slowly heavy method of acid of iso-electric point, slowly regulate pH to 4.5 ~ 4.7 of milk after the degreasing with acid solution; Keep deposition in 8000g, 4 ℃ of down centrifugal 20～30min; Distilled water wash 2 times promptly obtains rough casein powder, and freeze-drying is subsequent use;
2. slowly acid is heavy with iso-electric point, and the casein powder that obtains after the freeze-drying is mixed with 2% casein solution, adopts alkaline solution slowly to regulate pH to 7.0 ~ 7.5 of casein solution;
3. according to the Ca of 0.06mol
2+Concentration adds the divalence soluble calcium salt in the casein solution that 2. step obtains; In 8000g, 4 ℃ of following centrifugal 20～30min; Keep supernatant and deposition, deposition is dissolved in the disodium ethylene diamine tetra-acetic acid solution, dialysis back freeze-drying promptly obtains massfraction and reaches 91.6% α
s-casein;
4. 3. continue to add the divalence soluble calcium salt in the supernatant of centrifugal reservation in step, make Ca
2+The concentration final concentration arrives 0.3mol; Recentrifuge keeps supernatant and deposition, obtains massfraction after the supernatant dialysis freeze-drying and reaches κ-casein of 43.3%; Deposition is dissolved in the disodium ethylene diamine tetra-acetic acid solution, and dialysis back freeze-drying promptly obtains massfraction and reaches 100% beta-casein.
2. the method for Caseinum componemt in the separation buffalo milk as claimed in claim 1 is characterized in that: said casein is the casein of buffalo milk and buffalo milk goods.
3. the method for Caseinum componemt in the separation buffalo milk as claimed in claim 2 is characterized in that: said buffalo milk is that the purebred buffalo milk of mora, mora first-filial generation buffalo milk, mora hybridization height are for buffalo milk; Said buffalo milk goods are buffalo milk yogurt, buffalo milk cheese, buffalo milk milk powder.
4. the method for Caseinum componemt in the separation buffalo milk as claimed in claim 2 is characterized in that: said divalence soluble calcium salt is a calcium chloride.
5. the method for Caseinum componemt in the separation buffalo milk as claimed in claim 2 is characterized in that: said disodium ethylene diamine tetra-acetic acid solution is that EDTA Disodium is mixed with 0.06～0.3mol solution.
6. the method for Caseinum componemt in the separation buffalo milk as claimed in claim 2 is characterized in that: said acid solution is that glacial acetic acid or hydrochloric acid are mixed with 0.05～0.1mol solution.
7. the method for Caseinum componemt in the separation buffalo milk as claimed in claim 2 is characterized in that: said alkaline solution is that sodium hydroxide or ammoniacal liquor are mixed with 0.1～0.2mol solution.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105695542A (en) * | 2016-03-11 | 2016-06-22 | 江南大学 | Method for preparing partially dephosphorylated bovine casein to simulate phosphorylation level of human casein |
| CN108181399A (en) * | 2018-01-23 | 2018-06-19 | 新希望乳业股份有限公司 | The detection method of A2- beta-casein contents in a kind of dairy products |
| CN108191967A (en) * | 2018-01-23 | 2018-06-22 | 新希望双喜乳业（苏州）有限公司 | The separation method of A2- beta-caseins in a kind of milk |
| CN110526963A (en) * | 2019-09-24 | 2019-12-03 | 浙江工商大学 | A kind of non-enzymatic hydrolysis hypoallergenic casein preparation method for inhibiting antigen that submission is immunized |
| CN113061173A (en) * | 2021-03-18 | 2021-07-02 | 江南大学 | Method for separating alpha s 1-casein |
| CN113461795A (en) * | 2021-07-05 | 2021-10-01 | 湖北百特威生物科技有限公司 | Casein fractional precipitation purification method |
| CN116987170A (en) * | 2023-08-01 | 2023-11-03 | 广州绿萃生物科技有限公司 | Beta-casein and preparation method and application thereof |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105695542A (en) * | 2016-03-11 | 2016-06-22 | 江南大学 | Method for preparing partially dephosphorylated bovine casein to simulate phosphorylation level of human casein |
| CN108181399A (en) * | 2018-01-23 | 2018-06-19 | 新希望乳业股份有限公司 | The detection method of A2- beta-casein contents in a kind of dairy products |
| CN108191967A (en) * | 2018-01-23 | 2018-06-22 | 新希望双喜乳业（苏州）有限公司 | The separation method of A2- beta-caseins in a kind of milk |
| WO2019144642A1 (en) * | 2018-01-23 | 2019-08-01 | 新希望双喜乳业（苏州）有限公司 | Separation method for a2-β-casein in milk |
| CN108181399B (en) * | 2018-01-23 | 2019-08-13 | 新希望乳业股份有限公司 | The detection method of A2- beta-casein content in a kind of dairy products |
| CN110526963A (en) * | 2019-09-24 | 2019-12-03 | 浙江工商大学 | A kind of non-enzymatic hydrolysis hypoallergenic casein preparation method for inhibiting antigen that submission is immunized |
| CN113061173A (en) * | 2021-03-18 | 2021-07-02 | 江南大学 | Method for separating alpha s 1-casein |
| CN113061173B (en) * | 2021-03-18 | 2022-11-04 | 江南大学 | Separation alpha s1 Method for producing casein |
| CN113461795A (en) * | 2021-07-05 | 2021-10-01 | 湖北百特威生物科技有限公司 | Casein fractional precipitation purification method |
| CN113461795B (en) * | 2021-07-05 | 2022-08-26 | 湖北百特威生物科技有限公司 | Casein fractional precipitation purification method |
| CN116987170A (en) * | 2023-08-01 | 2023-11-03 | 广州绿萃生物科技有限公司 | Beta-casein and preparation method and application thereof |
| CN116987170B (en) * | 2023-08-01 | 2024-04-05 | 广州绿萃生物科技有限公司 | Beta-casein and preparation method and application thereof |
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