CN102674647A - Method for reactivating denitrification activity of inactivated anammox sludge - Google Patents
Method for reactivating denitrification activity of inactivated anammox sludge Download PDFInfo
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- CN102674647A CN102674647A CN2012101657859A CN201210165785A CN102674647A CN 102674647 A CN102674647 A CN 102674647A CN 2012101657859 A CN2012101657859 A CN 2012101657859A CN 201210165785 A CN201210165785 A CN 201210165785A CN 102674647 A CN102674647 A CN 102674647A
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Abstract
The invention discloses a method for reactivating the denitrification activity of inactivated anammox sludge. The method comprises the following steps: taking out the inactivated sludge and placing into a magnetoelectric induction reactor; performing activation treatment, namely standing, settling, discharging the supernate, adding newly prepared nutritional liquid, mixing by an internal circulating stirring method, preparing an electric field, a magnetic field and the action mode program respectively, and performing magnetoelectric induction treatment sequentially according to low intensity simultaneous action, medium and low intensity alternating action and high intensity simultaneous action; exchanging the nutritional liquid for one time every other day during three days before activation treatment; exchanging the nutritional liquid for one time every day after activation treatment; and stopping induction treatment when denitrification reaction occurs. By the method, the denitrification activity of the inactivated anammox bacteria can be reactivated, the original high-efficiency denitrification and regeneration capacities recover gradually, or the activity of a culture with reduced activity is recovered.
Description
Technical field
The invention belongs to the Environmental Biotechnology field, be specifically related to a kind of method of using magnetoelectric effect activation inactivation anaerobic ammonium oxidizing bacteria denitrification activity, keeping mud efficient denitrification usefulness.
Background technology
Anammox (Anammox) is the new bio denitrification process of developing in recent years; When receiving lot of domestic and international scholar's concern because of its high denitrification efficiency with uniqueness; Also because of its anaerobic ammonia oxidizing bacteria poor growth, inactivation and being difficult on the engineering is widely used easily.Therefore, Many researchers mainly is devoted to energy to study the method for quick active inactivation anaerobic ammonia oxidizing bacteria for addressing this problem at present.
And aspect anaerobic ammonia oxidation process research, engineering strain is carried out mutagenic treatment both at home and abroad and adopt single or compounding method often.High-voltage electric field that use adds and magnetic field; Through the stress response of biological dielectric substance to the compound magnetoelectric field, confirm magnetoelectricity radiating biological effect in experiment, obtain " frequency effect window ", " power (amplitude) the effect window " and " window action time " of certain bacterial strain; Find the low-frequency effects rule; Activation or rejuvenation are losing certain active bacterial strain gradually, make bacterial strain recover active again, keep its certain useful active state; To its specific trait of human use is human productive life service, is a difficult problem that often runs into during mikrobe utilizes and need to solve.Through magnetoelectricity inductive method the bacterial strain of the anaerobic ammonium oxidizing bacteria of inactivation is activated processing, can keep the denitrification activity of anaerobic ammonium oxidizing bacteria to remain on the heavy-duty service state for a long time, service is provided for the mankind administer nitrogenous effluent.
Need find a kind of method that is used to activate inactivation Anammox denitrogenation sludge activity; Being primarily aimed at the anaerobic ammonium oxidizing bacteria denitrification activity can lose by the generation denitrification activity after operation for some time gradually; Prilling mud disintegrates, flora color change, the phenomenon that the part thalline is dead; If can not in time activate processing, might cause the consequence of whole bacterial death.
Summary of the invention
Technical problem to be solved by this invention provides a kind of method that activates inactivation Anammox denitrogenation sludge activity again.
The technical scheme that realizes the object of the invention is:
A kind of method that activates inactivation Anammox denitrogenation sludge activity again comprises the steps:
(1) magnetoelectricity that has electric field to produce system and magnetic field generation system is set and induces bio-reactor;
(2) take out the anaerobic ammoxidation activity mud of inactivation, be positioned over magnetoelectricity and induce in the bio-reactor;
(3) discharge supernatant behind the quiescent setting;
(4) add the nutritive medium of newly joining;
(5) adopt the internal recycle stirring means to mix;
(6) dispose strength of electric field, magneticstrength respectively, the mode of action program in electric field, magnetic field;
(7) at preceding 3 days that activate processing, change one time of nutrition liquid every other day; Change one time of nutrition liquid later on every day; Mode with even flow after 7-10 days provides nutritive medium;
(8) treat that it can stop to induce processing in the time of carrying out denitrification reaction, induction duration is 10 to 30 days.
Step (4) is said to be ammonia the salt [(NH in the nutritive medium
4)
2SO
4] concentration is 40mg/L, nitrite (NaNO
2) concentration is 30mg/L, the Inlet and outlet water flow is 2ml/s, is prior art.
The said configuration strength of electric field of step (6), magneticstrength, the mode of action program in electric field, magnetic field is:
Be treated to one-period (1) one day 3 times, be separated by 2-6 hour at every turn;
(2) mode that acts on simultaneously by low strength is for the first time carried out magnetoelectricity and is induced processing, handles whipping appts in starting simultaneously 1-3 hour;
(3) carry out magnetoelectricity by the mode of middle intensity alternating action for the second time and induce processing, by turns once co-processing 1-3 hour, started the internal recycle whipping appts simultaneously in per 20 minutes;
(4) mode that acts on simultaneously by HS is for the third time carried out magnetoelectricity and is induced processing, handles 1-3 hour, starts the internal recycle whipping appts simultaneously;
The described strength of electric field of step (6) is 2.5-50v/M, magneticstrength: 40-360mT.
The invention has the beneficial effects as follows:
Through carrying out above several steps; Under the comprehensive action of magnetoelectric effect; The physiological and biochemical procedure that is in the anaerobic ammonium oxidizing bacteria of inactivation is reversed; Some bacterium obtains through transformation or regains denitrification activity, makes that the denitrification activity of bacterium is activated again, reverses inactivation.Thereby this method can be used for activating that denitrification activity reduces just gradually or the anaerobic ammonia oxidizing bacteria of inactivation, provides competent mud to guarantee for ensureing the stable of Anammox denitrification process;
In addition, the activity that present method also can be used to study the anaerobic ammonia oxidizing bacteria under magnetic field, electric field or the magnetoelectricity field while/alternating action activates the biological mechanism of transition process, the anaerobic ammonia oxidizing bacteria vital movement state under comparative study activation process and the normal state; Help further exploring each item action condition in the activation process,, obtaining better operational factors, reference frame is provided for the optimization of Activiation method improves like the righttest strength of electric field, the righttest magneticstrength.
Embodiment
The realization of this patent is described with specific embodiment below.
Embodiment 1
1, takes out the anaerobic ammoxidation activity mud 30L that denitrification activity begins decline (denitrification activity is lower than normal value more than 10%), be positioned over magnetoelectricity and induce in the bio-reactor;
2, discharge supernatant behind the quiescent setting 6h;
3, add the nutritive medium of newly joining, ammonia salt [(NH in the nutritive medium
4)
2SO
4] concentration is 40mg/L, nitrite (NaNO
2) concentration is 30mg/L, the Inlet and outlet water flow is 2ml/s;
4, adopt the internal recycle stirring means to carry out thorough mixing 30min;
5, dispose strength of electric field, magneticstrength and mode of action program respectively:
5.1 one day 3 times is a processing cycle, is separated by 3 hours at every turn;
5.2 for the first time by low strength (strength of electric field: 2.5v/M; Magneticstrength: 40mT; ) mode of effect simultaneously carries out magnetoelectricity and induce processing, handles 1 hour, starts the internal recycle whipping appts simultaneously;
5.3 for the second time by middle intensity (strength of electric field: 10v/M; Magneticstrength: 120mT; ) mode of alternating action carries out magnetoelectricity and induce processing, by turns once co-processing 1 hour started the internal recycle whipping appts simultaneously in per 20 minutes;
5.4 for the third time by HS (strength of electric field: 40v/M; Magneticstrength: 360mT; ) mode of effect simultaneously carries out magnetoelectricity and induce processing, handles 1 hour, starts the internal recycle whipping appts simultaneously;
6, at preceding 3 days that activate processing, change one time of nutrition liquid every other day; Change one time of nutrition liquid every day after 3 days; Adopt the method for even flow to improve nutritive medium after seven days, the Inlet and outlet water flow is 2ml/s.
7, treat that it can stop to induce processing in the time of carrying out denitrification reaction, induction duration is 10 days.
Through detecting, activity ratio is 80%.
Embodiment 2
1, takes out the anaerobic ammoxidation activity mud 30L that denitrification activity reduces 25%-70%, be positioned over magnetoelectricity and induce in the bio-reactor;
2, discharge supernatant behind the quiescent setting 6h;
3, add the nutritive medium of newly joining, ammonia salt [(NH in the nutritive medium
4)
2SO
4] concentration is 45mg/L, nitrite (NaNO
2) concentration is 35mg/L;
4, adopt the internal recycle stirring means to carry out thorough mixing 30min;
5, dispose strength of electric field, magneticstrength and mode of action program respectively:
5.1 one day 3 times is a processing cycle, is separated by 4 hours at every turn;
5.2 for the first time by low strength (strength of electric field: 3v/M; Magneticstrength: 40mT; ) mode of effect simultaneously carries out magnetoelectricity and induce processing, handles 2 hours, starts the internal recycle whipping appts simultaneously;
5.3 for the second time by middle intensity (strength of electric field: 12v/M; Magneticstrength: 120mT; ) mode of alternating action carries out magnetoelectricity and induce processing, by turns once co-processing 2 hours started the internal recycle whipping appts simultaneously in per 20 minutes;
5.4 for the third time by HS (strength of electric field: 48v/M; Magneticstrength: 360mT; ) mode of effect simultaneously carries out magnetoelectricity and induce processing, handles 2 hours, starts the internal recycle whipping appts simultaneously;
6, at preceding 3 days that activate processing, change one time of nutrition liquid every other day; Change one time of nutrition liquid every day after 3 days; Adopt the method for even flow to improve nutritive medium after ten days, the Inlet and outlet water flow is 2ml/s.
7, treat that it can stop to induce processing in the time of carrying out denitrification reaction, induction duration is 20 days.
Through detecting, activity ratio is 70%.
Embodiment 3
1, takes out the anaerobic ammoxidation activity mud 30L of inactivation (denitrification activity reduces above 70%), be positioned over magnetoelectricity and induce in the bio-reactor;
2, discharge supernatant behind the quiescent setting 6h;
3, add the nutritive medium of newly joining, ammonia salt [(NH in the nutritive medium
4)
2SO
4] concentration is 45mg/L, nitrite (NaNO
2) concentration is 35mg/L;
4, adopt the internal recycle stirring means to carry out thorough mixing 30min;
5, dispose strength of electric field, magneticstrength and mode of action program respectively:
5.1 one day 3 times is a processing cycle, is separated by 5 hours at every turn
5.2 for the first time by low strength (strength of electric field: 3v/M; Magneticstrength: 40mT; ) mode of effect simultaneously carries out magnetoelectricity and induce processing, handles 1 hour, starts the internal recycle whipping appts simultaneously;
5.3 for the second time by middle intensity (strength of electric field: 10v/M; Magneticstrength: 120mT; ) mode of alternating action carries out magnetoelectricity and induce processing, by turns once co-processing 3 hours started the internal recycle whipping appts simultaneously in per 20 minutes;
5.4 for the third time by HS (strength of electric field: 50v/M; Magneticstrength: 360mT; ) mode of effect simultaneously carries out magnetoelectricity and induce processing, handles 3 hours, starts the internal recycle whipping appts simultaneously;
6, at preceding 3 days that activate processing, change one time of nutrition liquid every other day; Change one time of nutrition liquid every day after 3 days; Adopt the method for even flow to improve nutritive medium after ten days, the Inlet and outlet water flow is 2ml/s.
7, treat that it can stop to induce processing in the time of carrying out denitrification reaction, induction duration is 25-30 days.
Through detecting activity ratio about 65%.
Claims (3)
1. a method that activates inactivation Anammox denitrogenation sludge activity again is characterized in that: comprise the steps:
(1) magnetoelectricity that has electric field to produce system and magnetic field generation system is set and induces bio-reactor;
(2) take out the anaerobic ammoxidation activity mud of inactivation, be positioned over magnetoelectricity and induce in the bio-reactor;
(3) discharge supernatant behind the quiescent setting;
(4) add the nutritive medium of newly joining;
(5) adopt the internal recycle stirring means to mix;
(6) dispose strength of electric field, magneticstrength respectively, the mode of action program in electric field, magnetic field;
(7) at preceding 3 days that activate processing, change one time of nutrition liquid every other day; Change one time of nutrition liquid later on every day;
(8) treat that it can stop to induce processing in the time of carrying out denitrification reaction, induction duration is 10 to 30 days.
2. method according to claim 1 is characterized in that: the said configuration strength of electric field of step (6), magneticstrength, and the mode of action program in electric field, magnetic field is:
Be treated to one-period (1) one day 3 times, be separated by 2-6 hour at every turn;
(2) mode that acts on simultaneously by low strength is for the first time carried out magnetoelectricity and is induced processing, handles whipping appts in starting simultaneously 1-3 hour;
(3) carry out magnetoelectricity by the mode of middle intensity alternating action for the second time and induce processing, by turns once co-processing 1-3 hour, started the internal recycle whipping appts simultaneously in per 20 minutes;
(4) mode that acts on simultaneously by HS is for the third time carried out magnetoelectricity and is induced processing, handles 1-3 hour, starts the internal recycle whipping appts simultaneously.
3. method according to claim 1 and 2 is characterized in that: the described strength of electric field of step (6) is 2.5-50v/M, magneticstrength: 40-360mT.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031905A (en) * | 2014-06-24 | 2014-09-10 | 大连理工大学 | Method for enhancing anaerobic ammonia oxidation bacterial activity by virtue of electric field |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102336472A (en) * | 2011-09-08 | 2012-02-01 | 大连理工大学 | Electrically enhanced ANAMMOX biologic nitrogen removal method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102336472A (en) * | 2011-09-08 | 2012-02-01 | 大连理工大学 | Electrically enhanced ANAMMOX biologic nitrogen removal method |
Non-Patent Citations (4)
| Title |
|---|
| SITONG LIU等: "Enhanced anammox consortium activity for nitrogen removal:Impacts of static magnetic field", 《JOURNAL OF BIOTECHNOLOGY》, no. 138, 31 December 2008 (2008-12-31), pages 96 - 102 * |
| YAOBIN ZHANG等: "Enhancement of sludge graulation in a zero valence iron packed anaerobic reactor with a hydraulic circulation", 《PROCESS BIOCHEMISTRY》, no. 46, 31 December 2011 (2011-12-31), pages 471 - 476 * |
| YIWEN LIU等: "Applying an electric field in a built-in zero valent iron-Anaerobic reactor for enhancement of sludge granulation", 《WATER RESEARCH》, no. 45, 31 December 2011 (2011-12-31), pages 1258 - 1266, XP027577674 * |
| 刘思彤: "电/磁场强化厌氧氨氧化及多菌群协同自养生物脱氮", 《中国博士学位论文全文数据库工程科技Ⅰ辑》, no. 10, 15 October 2009 (2009-10-15), pages 027 - 37 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031905A (en) * | 2014-06-24 | 2014-09-10 | 大连理工大学 | Method for enhancing anaerobic ammonia oxidation bacterial activity by virtue of electric field |
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