CN102668902B - 一种评价堆肥对土传病害抑制能力的方法 - Google Patents

一种评价堆肥对土传病害抑制能力的方法 Download PDF

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CN102668902B
CN102668902B CN 201210191828 CN201210191828A CN102668902B CN 102668902 B CN102668902 B CN 102668902B CN 201210191828 CN201210191828 CN 201210191828 CN 201210191828 A CN201210191828 A CN 201210191828A CN 102668902 B CN102668902 B CN 102668902B
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和苗苗
田光明
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Abstract

本发明涉及一种评价堆肥对土传病害抑制能力的方法。本发明首先准备需要评价的腐熟堆肥;在土壤温度相对稳定3~5天的条件下,测定土传病害根腐病在土壤中的背景值ICK;采集0~20cm土层土壤,在土壤中添加腐熟堆肥,恒温20~25℃放置k天;计算土壤根腐病发病率Im,并计算第m次取出盆栽容器的土壤根腐病发病率与背景值的比值IRm;对k+1个IRm数据分析后获得短期波动特征来评价堆肥对土壤土传病害抑制能力。本发明有利于促进堆肥质量提高、保护土壤生态系统平衡,特别是温室土壤,为一种评价堆肥对土传病害抑制能力的有效方法。

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一种评价堆肥对土传病害抑制能力的方法
技术领域
本发明属于生物环保技术领域,涉及一种评价堆肥对土传病害抑制能力的方法。
背景技术
在集约化耕作、频繁轮作的农业体系下,土壤健康受到极大压力,肥力降低、病害严重、生态系统自身恢复力差。施用腐熟堆肥可明显抑制或降低土传病害的发病率,堆肥通过自身包含的拮抗微生物或增加土壤碳、氮等养分、促进微生物的生长和种群多样性,来抵抗土传病害并降低其发病率。因此,对土传病害的抑制能力已成为评价堆肥品质的重要参数。
目前,对于堆肥的品质人们主要关注堆肥的腐熟度,而对堆肥抗病虫害能力关注不多,专门用来评价堆肥对土传病害抑制能力的方法很少,大多是将堆肥投加进土壤后观察发病情况,或者利用生物学技术分析堆肥中拮抗微生物的数量。实际上,这并不能很好地说明堆肥对土传病害的抑制能力,由于土壤中一些病菌对土壤碳、氮的响应很迅速,堆肥施入后生源要素含量显著增加,病菌会在短期内与土壤微生物竞争新鲜有机质,进而影响堆肥对土传病菌的抑制能力。当土壤微生物快速繁殖、活性增强时,土传病菌的发病率降低;当土壤微环境暂时缺氧、微生物逐渐死亡、数量降低时,土传病菌的发病率升高;当氧气重新达到充足状态且微生物残体成为新的营养源时,微生物又立即快速繁殖,土传病菌的发病率又降低,如此重复,直到稳定。可见,土传病原菌的发病率在堆肥施用后呈短期波动变化。
发明内容  
本发明的目的是提供一种评价堆肥对土传病害抑制能力的方法。
本发明方法的具体步骤是:
步骤(1).准备需要评价的腐熟堆肥;
所述的腐熟堆肥的腐熟要求是:外观呈茶褐色或者暗黑色、呈现疏松团粒结构、无恶臭,碳氮比低于15;
步骤(2).在土壤温度相对稳定3~5天的条件下,测定土传病害根腐病(Pythium)在土壤中的背景值,测定方法为将土壤装入n个盆栽容器中,在每个盆栽容器土壤表面播种S颗黄瓜种子,S=20~40,每隔2~3天浇一次水,14天后观测未出苗数与倒苗数,并计算土壤根腐病发病率的背景值I CK
I CKi=(未出苗数CKi +倒苗数CKi)/ S×100﹪,
其中i为第i个盆栽容器,则I CK=∑I CKi/n;
步骤(3).采集0~20cm土层土壤,在土壤中添加腐熟堆肥,添加量为土壤干重的1﹪(w/w),混合均匀后分别装入各盆栽容器中,恒温20~25℃放置k天;
步骤(4).土壤与腐熟堆肥混合后立即取出1组盆栽容器(第0天),以后每间隔24小时再取出1组盆栽容器;每次取出盆栽容器后,每盆盆栽容器中播种 S′颗黄瓜种子到土壤表面,S′=20~40,14天后观察未出苗数与倒苗数,并计算土壤根腐病发病率Im,每组盆栽容器为3个盆栽容器;
I mj=(未出苗数mj +倒苗数mj)/ S′×100﹪,
其中m为第m次取出盆栽容器、j为第j组盆栽容器,则Im=∑I mj/3;
步骤(5).计算腐熟堆肥施入后第m次取出盆栽容器的土壤根腐病发病率与背景值的比值IRm,即IRm=Im/ICK,对 k+1个IRm数据分析后获得短期波动特征来评价堆肥对土壤土传病害抑制能力,评价方法如下:
a. 如果小于1的IRm数值有大于k/2个,波动周期规律、每个波动幅度相似或逐渐降低,表明堆肥对土壤土传病害的抑制能力强、土壤健康程度高;
b. 如果小于1的IRm数值有大于k/2个,但波动周期不规律、波动幅度不稳定,表明堆肥对土壤土传病害的抑制能力强、但土壤健康程度低;
c. 如果小于1的IRm数值有小于等于k/2个,波动周期规律、每个波动幅度相似或逐渐降低,表明堆肥对土壤土传病害的抑制能力弱、土壤健康程度高;
d. 如果小于1的IRm数值有小于等于k/2个,波动周期不规律、波动幅度不稳定,表明堆肥对土壤土传病害的抑制能力弱、土壤健康程度低。
本发明所具有的有益效果:
本发明利用土壤生态学理论,通过考察腐熟堆肥施用后土传病原菌发病率的短期波动特征来评价堆肥对土传病害的抑制能力,有利于保护土壤健康、提高堆肥质量。
本发明通过动态监测腐熟堆肥施入后土壤中土传病原菌发病率的短期波动变化,并分析短期波动特征来判断堆肥对土传病害的抑制能力,这有利于促进堆肥质量提高、保护土壤生态系统平衡,特别是温室土壤,为一种评价堆肥对土传病害抑制能力的有效方法。
附图说明
图1是牛粪堆肥施入土壤后根腐病发病率与背景值的比值IRm随时间的短期变化示意图;
图2是绿肥施入土壤后根腐病发病率与背景值的比值IRm随时间的短期变化示意图;
图3是污泥堆肥分别施入休耕土壤后根腐病发病率与背景值的比值IRm随时间的短期变化示意图;
图4是污泥堆肥分别施入轮作土壤后根腐病发病率与背景值的比值IRm随时间的短期变化示意图。
具体实施方式
实施例1:
评价堆肥为牛粪与稻草好氧堆置而成的牛粪堆肥,土壤取自常年施用有机肥、连作玉米的农田。在土壤温度相对稳定3天的条件下,将土壤装入3盆0.6L的盆栽容器中,在每盆盆栽容器土壤表面播种20颗黄瓜种子,每隔60小时浇一次水,14天后观测未出苗数CK与倒苗数CK,计算土壤根腐病发病率背景值的平均值I CK=15.6﹪;
采集0~20cm土层土壤,在土壤中添加腐熟牛粪堆肥,添加量为土壤干重的1﹪(w/w),混合均匀后分别装入多盆0.6L的盆栽容器中,温室中恒温20℃放置20天;
从第0天开始(土壤与腐熟堆肥混合当天)每天取1组盆栽容器,每组盆栽容器为3个盆栽容器,将20颗黄瓜种子播种到土壤表面,14天后观察未出苗数与倒苗数,计算土壤根腐病发病率Im及土壤根腐病发病率与背景值的比值IRm
如图1所示,0~20天的IRm数据中小于1的IRm数值有17个,波动周期规律为4~5天,波动幅度随时间逐渐降低,表明堆肥对土壤土传病害的抑制能力强、该土壤的健康程度高。
实施例2:
评价堆肥为水稻秸秆好氧堆置成的绿肥,土壤为常年以化肥为基肥,复合肥为追肥的水稻土。在土壤温度相对稳定4天的条件下,将土壤装入3盆0.6L的盆栽容器中,在土壤表面播种30颗黄瓜种子,每隔2天浇一次水,14天后观测未出苗数CK与倒苗数CK,计算土壤根腐病发病率背景值的平均值I CK=22.2﹪;
采集0~20cm土层土壤,在土壤中添加腐熟绿肥,添加量为土壤干重的1﹪(w/w),混合均匀后分别装入若干0.6L的盆栽容器中,温室中恒温23℃放置20天;
从第0天开始(土壤与腐熟堆肥混合当天)每天取1组盆栽容器,每组盆栽容器为3个盆栽容器,将30颗黄瓜种子播种到土壤表面,14天后观察未出苗数与倒苗数,计算土壤根腐病发病率I m及土壤根腐病发病率与背景值的比值IRm
如图2所示,0~20天的IRm数据中小于1的IRm数值有14个,但波动周期不规律、波动幅度不稳定,表明堆肥对土壤土传病害的抑制能力强、但该土壤健康程度低。
实施例3:
评价堆肥为污水污泥与木屑好氧堆置成的污泥堆肥,土壤为休耕1年的土壤(休耕前黄瓜-辣椒-叶菜轮作。在土壤温度相对稳定5天的条件下,将土壤装入3盆0.6L的盆栽容器中,在土壤表面播种40颗黄瓜种子,每隔2天浇一次水,14天后观测未出苗数CK与倒苗数CK,计算休耕土壤根腐病发病率背景值的平均值I CK为8.9﹪;
采集0~20cm土层土壤,在土壤中添加腐熟污泥堆肥,添加量为土壤干重的1﹪(w/w),混合均匀后分别装入若干0.6L的盆栽容器中,温室中恒温25℃放置20天;
从第0天开始(土壤与腐熟堆肥混合当天)每天取1组盆栽容器,每组盆栽容器为3个盆栽容器,将40颗黄瓜种子播种到土壤表面,14天后观察休耕土壤与轮作土壤中的未出苗数与倒苗数,计算土壤根腐病发病率I m以及土壤根腐病发病率与背景值的比值IRm
如图3所示,对于休耕土壤,0~20天的IRm数据中小于1的IRm数值有9个,波动周期规律为4~5天,波动幅度随时间逐渐降低,表明堆肥对土壤土传病害的抑制能力弱、但该土壤健康程度较高。
实施例4:
评价堆肥为污水污泥与木屑好氧堆置成的污泥堆肥,土壤为黄瓜-辣椒-叶菜轮作的土壤。在土壤温度相对稳定5天的条件下,将土壤装入3盆0.6L的盆栽容器中,在土壤表面播种40颗黄瓜种子,每隔2天浇一次水,14天后观测未出苗数CK与倒苗数CK,计算轮作土壤根腐病发病率背景值的平均值I CK为18.9﹪;
采集0~20cm土层土壤,在土壤中添加腐熟污泥堆肥,添加量为土壤干重的1﹪(w/w),混合均匀后分别装入若干0.6L的盆栽容器中,温室中恒温25℃放置20天;
从第0天开始(土壤与腐熟堆肥混合当天)每天取1组盆栽容器,每组盆栽容器为3个盆栽容器,将40颗黄瓜种子播种到土壤表面,14天后观察轮作土壤中的未出苗数与倒苗数,计算土壤根腐病发病率I m以及土壤根腐病发病率与背景值的比值IRm
如图4所示,对于轮作土壤,0~20天的IRm数据中小于1的IRm数值有7个,但波动周期不规律、波动幅度不稳定,表明堆肥对土壤土传病害的抑制能力弱、该土壤健康程度较低。

Claims (1)

1. 一种评价堆肥对土传病害抑制能力的方法,其特征在于该方法的具体步骤是:
步骤(1).准备需要评价的腐熟堆肥;
所述的腐熟堆肥的腐熟要求是:外观呈茶褐色或者暗黑色、呈现疏松团粒结构、无恶臭,碳氮比低于15;
步骤(2).在土壤温度相对稳定3~5天的条件下,测定土传病害根腐病在土壤中的背景值,测定方法为将土壤装入n个盆栽容器中,在每个盆栽容器土壤表面播种S颗黄瓜种子,S=20~40,每隔2~3天浇一次水,14天后观测未出苗数与倒苗数,并计算土壤根腐病发病率的背景值I CK
I CKi=(未出苗数CKi +倒苗数CKi)/ S×100﹪,
其中i为第i个盆栽容器,则I CK=∑I CKi/n;
步骤(3).采集0~20cm土层土壤,在土壤中添加腐熟堆肥,添加量为土壤干重的1﹪,混合均匀后分别装入各盆栽容器中,恒温20~25℃放置k天;
步骤(4).土壤与腐熟堆肥混合后立即取出1组盆栽容器,以后每间隔24小时再取出1组盆栽容器;每次取出盆栽容器后,每盆盆栽容器中播种 S′颗黄瓜种子到土壤表面,S′=20~40,14天后观察未出苗数与倒苗数,并计算土壤根腐病发病率Im,每组盆栽容器为3个盆栽容器;
I mj=(未出苗数mj +倒苗数mj)/ S′×100﹪,
其中m为第m次取出盆栽容器、j为第j组盆栽容器,则Im=∑I mj/3;
步骤(5).计算腐熟堆肥施入后第m次取出盆栽容器的土壤根腐病发病率与背景值的比值IRm,,即IRm=Im/ICK,对 k+1个IRm数据分析后获得短期波动特征来评价堆肥对土壤土传病害抑制能力,评价方法如下:
a. 如果小于1的IRm数值有大于k/2个,波动周期规律、每个波动幅度相似或逐渐降低,表明堆肥对土壤土传病害的抑制能力强、土壤健康程度高;
b. 如果小于1的IRm数值有大于k/2个,但波动周期不规律、波动幅度不稳定,表明堆肥对土壤土传病害的抑制能力强、但土壤健康程度低;
c. 如果小于1的IRm数值有小于等于k/2个,波动周期规律、每个波动幅度相似或逐渐降低,表明堆肥对土壤土传病害的抑制能力弱、土壤健康程度高;
d. 如果小于1的IRm数值有小于等于k/2个,波动周期不规律、波动幅度不稳定,表明堆肥对土壤土传病害的抑制能力弱、土壤健康程度低。
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CN102165865B (zh) * 2010-12-30 2012-10-03 华南农业大学 一种利用本土蔬菜种子测定城市污泥有机肥腐熟度的方法

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