CN102645755B - Near field multi-optical trapping device and method - Google Patents
Near field multi-optical trapping device and method Download PDFInfo
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- CN102645755B CN102645755B CN201210107333.5A CN201210107333A CN102645755B CN 102645755 B CN102645755 B CN 102645755B CN 201210107333 A CN201210107333 A CN 201210107333A CN 102645755 B CN102645755 B CN 102645755B
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Abstract
The invention relates to a near field multi-optical trapping device and method. The device comprises a first light source, a polarizing converter, a beam expanding system, a first beam splitter mirror and a focusing field, wherein the first light source is used for emitting parallel light beams to the polarizing converter; the polarizing converter is used for converting the parallel light beams into high-order axially-symmetric polarized light beams and transmitting to the beam expanding system; the beam expanding system is used for expanding the high-order axially-symmetric polarized light beams into light spots of set sizes and transmitting into the first beam splitter mirror; the first beam splitter mirror is used for reflecting the light spots of set sizes and transmitting onto a Kretschmann structure consisting of an oil-submerged object lens, a glass substrate and a metal film; the focusing field is formed by transmitting the light spots of set sizes onto the oil-submerged object lens and focusing onto an interface between the glass substrate and the metal film; the light spots of set sizes are used for exciting surface plasma waves on the surface of the metal film; and the light field of the surface plasma waves and medium particles near the metal surface interact, so that particle trapping is realized. According to the device and the method, the quantity and scales of trapped particles can be regulated and controlled flexibly.
Description
Technical field
The present invention relates to optical technical field, particularly a kind of many optical acquisitions of near field device and method.
Background technology
Optical acquisition (Optical Trapping) is a kind of technology of utilizing the radiant force manipulation particle of focused beam generation.This technology starts from the seventies in last century, through the development of decades, has been proved to be a kind of very effective instrument, is widely used in multiple fields such as biology, medical science, chemistry and physics.1970, the A.Ashkin first report of the U.S.'s Bell Laboratory light beam can produce scattering force and gradient force to the particle of micro-meter scale, and subsequently experiment shows in 1986 a kind of single beam gradient force optical acquisition device, i.e. said smooth tweezer (Optical Tweezers) now.
For further strengthening the function of light tweezer, some the many optical acquisitions technology that can simultaneously catch multiple particles are proposed successively, for example: utilize diffraction optical element, microlens array, interfering beam, Vcsel (VCSEL) array or fiber array to obtain multiple focuses (Hot Spots) simultaneously, can catch multiple particles simultaneously.But inventor finds, prior art majority is to use high-NA objective in light tweezer, thereby because quantity and the size of trapped particle have been limited in the limited visual field of high-NA objective.
Summary of the invention
The object of the present invention is to provide a kind of many optical acquisitions of near field device and method, realize quantity and the yardstick of regulation and control trapped particle flexibly.
The embodiment of the present invention provides a kind of many optical acquisitions of near field device, comprising:
The first light source, for to polarization converter emitting parallel light bundle;
Described polarization converter, for described parallel beam being converted to the rotational symmetry light beam of senior time, and is emitted to beam-expanding system by the rotational symmetry light beam of described senior time;
Described beam-expanding system, for the rotational symmetry light beam of described senior time is expanded into the hot spot of setting size, and is incident to the first beam splitter by hot spot big or small described setting;
Described the first beam splitter is by after hot spot reflection big or small described setting, incide in the Kretchman structure being formed by oil immersion objective, substrate of glass and metallic film, described substrate of glass and described metallic film are placed in a double dish, comprise the solution of setting insulating particles in described double dish;
The big or small hot spot of described setting incides the interface that focuses on described substrate of glass and metallic film after described oil immersion objective and forms focousing field; Wherein, the big or small hot spot of described setting goes out surface plasma-wave at the surface excitation of described metallic film; Near the insulating particles light field of described surface plasma-wave and described metal surface interact and realize particle-capture, described in the quantity of the insulating particles of catching determined by the focus quantity of described focousing field.
The embodiment of the present invention also provides a kind of many optical acquisitions of near field method, comprising:
By the first light source to polarization converter emitting parallel light bundle;
By described polarization converter, described parallel beam is converted to the rotational symmetry light beam of senior time, and the rotational symmetry light beam of described senior time is emitted to beam-expanding system;
By beam-expanding system, the rotational symmetry light beam of described senior time is expanded into the hot spot of setting size, and hot spot big or small described setting is incident to the first beam splitter;
After hot spot big or small described setting being reflected by described the first beam splitter, incide in the Kretchman structure being formed by oil immersion objective, substrate of glass and metallic film, described substrate of glass and described metallic film are placed in a double dish, comprise the solution of setting insulating particles in described double dish;
The big or small hot spot of described setting incides and focuses on the substrate of glass that is placed in double dish after described oil immersion objective and the interface of metallic film forms focousing field; Wherein, the big or small hot spot of described setting goes out surface plasma-wave at the surface excitation of described metallic film; Near the insulating particles light field of described surface plasma-wave and described metal surface interact and realize particle-capture, described in the quantity of the insulating particles of catching determined by the focus quantity of described focousing field.
Many optical acquisitions of near field device and method provided by the invention, the light field of the surface plasma-wave that the rotational symmetry light beam of senior time generating by the first light source and polarization converter can comprise multiple focuses at the surface excitation of metallic film simultaneously, thus realize multiparticle optical acquisition; In addition, the polarization state of adjusting the rotational symmetry light beam of senior time by polarization converter distributes, thereby can regulate and control flexibly the optical field distribution of the surface plasma-wave of the surface excitation of metallic film, change the focus quantity of the light field formation of surface plasma-wave, realize quantity and the yardstick of regulation and control trapped particle flexibly.
Brief description of the drawings
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, to the accompanying drawing of required use in embodiment or description of the Prior Art be briefly described below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is the schematic diagram of the space polarisation distribution of embodiment of the present invention rotational symmetry light beam;
Fig. 2 is the structural representation of an embodiment of many optical acquisitions of near field device of the present invention;
Fig. 3 is the structural representation of another embodiment of many optical acquisitions of near field device of the present invention;
Fig. 4 be according to p polarized light embodiment illustrated in fig. 3 reflectivity in substrate of glass/metallic film/medium system with the change curve schematic diagram of incident angle;
Fig. 5 is the distribution schematic diagram in metal surface x-y plane according to the SPP light field exciting in metal surface embodiment illustrated in fig. 3;
Fig. 6 is according to the distribution schematic diagram of the normal direction along metal surface embodiment illustrated in fig. 3;
Fig. 7 is the distribution schematic diagram along the gradient force of x axle according to two kinds of suffered radiant forces of different medium particle embodiment illustrated in fig. 3;
Fig. 8 is the gradient force distribution schematic diagram along z axle according to two kinds of suffered radiant forces of different medium particle embodiment illustrated in fig. 3;
Fig. 9 is the schematic flow sheet of an embodiment of many optical acquisitions of near field method of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiment.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
Near field optic described in the embodiment of the present invention is caught, and refers to the method for utilizing surface plasma (Surface Plasmon Polariton, referred to as SPP) the light-field capture particle (insulating particles) exciting at metal and medium interface; Because near field optic is caught and is had stronger two-dimension light field locality, need less luminous energy reaching catch power equally in the situation that, and preferably and other micro-optical device to carry out optics integrated.
Fig. 1 is the schematic diagram of the space polarisation distribution of embodiment of the present invention rotational symmetry light beam; As shown in Figure 1, the polarization state of light beam any point (except central point) on xsect is linear polarization, and polarization orientation is in xsect.If the xsect that xOy plane is light beam, the direction of propagation that z axle is light beam, S (r, φ) is certain a bit (except the central point) on beam cross-section, its polarization orientation meets following relation:
Φ(r，φ)＝P×φ+φ
0?(P≠0)
Wherein, P is polarization level time, represents the periodicity that when light beam along the circumferential direction changes 360 °, polarization orientation changes; φ
0for initial polarization azimuth corresponding when φ=0, its value is relevant with choosing of x axle; The polarization orientation that S the is ordered attitude corresponding with this point is relevant.In the time of the inferior P > 1 of polarization level, the embodiment of the present invention is referred to as the rotational symmetry light beam of senior time.
Fig. 2 is the structural representation of an embodiment of many optical acquisitions of near field device of the present invention; As shown in Figure 2, the embodiment of the present invention comprises: the first light source 20, polarization converter 21, beam-expanding system 22, the first beam splitter 23, Kretchman structure 24, double dish 25, wherein, Kretchman structure 24 is specifically made up of oil immersion objective 241, substrate of glass 242 and metallic film 243.
Particularly, the first light source 20 is to polarization converter 21 emitting parallel light bundles; Polarization converter 21 is converted to described parallel beam the rotational symmetry light beam of senior time, and the rotational symmetry light beam of described senior time is emitted to beam-expanding system 22; The rotational symmetry light beam of described senior time is expanded into the hot spot of setting size by beam-expanding system 22, and hot spot big or small described setting is incident to the first beam splitter 23; The first beam splitter 23 is by after hot spot reflection big or small described setting, incide in the Kretchman structure 24 being formed by oil immersion objective 241, substrate of glass 242 and metallic film 243, substrate of glass 242 and metallic film 2 are placed in double dish 25, and 25 li of double dish comprise the solution of setting insulating particles; The big or small hot spot of described setting incides the interface that focuses on substrate of glass 242 and metallic film 243 after oil immersion objective 241 and forms focousing field; Wherein, set big or small hot spot and go out surface plasma-wave at the surface excitation of metallic film 243; Near the insulating particles metal surface of the light field of described surface plasma-wave and metallic film 243 interact and realize particle-capture, described in the quantity of the insulating particles of catching determined by the focus quantity of described focousing field.
Many optical acquisitions of near field device that the embodiment of the present invention provides, the SPP light field that the rotational symmetry light beam of senior time generating by the first light source 20 and polarization converter 21 can comprise multiple focuses at the surface excitation of metallic film 243 simultaneously, thus realize multiparticle optical acquisition; In addition, the polarization state of adjusting the rotational symmetry light beam of senior time by polarization converter 21 distributes, thereby can regulate and control flexibly the SPP optical field distribution of the surface excitation of metallic film 243, change the focus quantity that SPP light field forms, realize quantity and the yardstick of regulation and control trapped particle flexibly.
Fig. 3 is the structural representation of another embodiment of many optical acquisitions of near field device of the present invention, as shown in Figure 3, the embodiment of the present invention comprises: the first light source 30, polarization converter 31, beam-expanding system 32, the first beam splitter 33, Kretchman structure 34, double dish 35, three-dimensional micro-displacement platform 36, secondary light source 37, collimation lens 38, the second beam splitter 39, charge coupled cell 40, be arranged on the condenser lens 41 between the second beam splitter 39 and charge coupled cell 40, wherein, Kretchman structure 34 is specifically by oil immersion objective, substrate of glass and metallic film composition, concrete structure as shown in Figure 2, at this not to oil immersion objective, substrate of glass and metallic film carry out label.Wherein, the first light source 30 specifically comprises: laser instrument 301, microcobjective 302, pin hole 303, collimation lens 304; Secondary light source 42 is specifically as follows illuminating lamp.
Particularly, laser instrument 301 sends light beam, and it is parallel beam that this light beam is collimated lens 304 collimations after microcobjective 302 and pin hole 303 filtering; Polarization converter 31 is converted to described parallel beam the rotational symmetry light beam of senior time, and the rotational symmetry light beam of described senior time is emitted to beam-expanding system 32; The rotational symmetry light beam of described senior time is expanded into the hot spot of setting size by beam-expanding system 32, and hot spot big or small described setting is incident to the first beam splitter 33; The first beam splitter 33 is by after hot spot reflection big or small described setting, incide in the Kretchman structure 34 being formed by oil immersion objective, substrate of glass and metallic film, substrate of glass 342 and metallic film 343 are placed in double dish 35, and 35 li of double dish comprise the solution of setting insulating particles; The big or small hot spot of described setting incides the interface that focuses on substrate of glass and metallic film after oil immersion objective and forms focousing field; Wherein, set big or small hot spot and go out surface plasma-wave at the surface excitation of metallic film; Near the insulating particles light field of described surface plasma-wave and the metal surface of metallic film interact and realize particle-capture, described in the quantity of the insulating particles of catching determined by the focus quantity of described focousing field.For the three-dimensional micro-displacement platform 36 of culture dish holding 35, by the displacement of the three-dimensional micro-displacement platform 36 of computer control.
Secondary light source 42 is arranged on the first beam splitter 33 belows; Collimation lens 38 is arranged between the first beam splitter 33 and secondary light source 42; On the insulating particles of catching described in the light beam that secondary light source 42 sends is irradiated to by the first beam splitter 33 after collimation lens 38 collimations; The second beam splitter 39 is arranged between oil immersion objective and the first beam splitter 33; The light beam that the light beam that secondary light source 42 sends leaks in Kretchman structure 34 is emitted to condenser lens 41 through the second beam splitter 39, for the light beam of described leakage is converged in to charge coupled cell 40; The light beam of described leakage is carried out imaging by charge coupled cell 40, thereby obtain the shape information of described insulating particles, and this shape information can be for example: shape of particle, size and particle relative tertiary location each other etc.
Many optical acquisitions of near field device that the embodiment of the present invention provides, the SPP light field that the rotational symmetry light beam of senior time generating by the first light source 30 and polarization converter 31 can comprise multiple focuses at the surface excitation of metallic film simultaneously, thus realize multiparticle optical acquisition; In addition, the polarization state of adjusting the rotational symmetry light beam of senior time by polarization converter 31 distributes, thereby can regulate and control flexibly the SPP optical field distribution of the surface excitation of metallic film, change the focus quantity that SPP light field forms, realize quantity and the yardstick of regulation and control trapped particle flexibly.
In order to be illustrated more clearly in the technical scheme of the embodiment of the present invention, be described in detail embodiment illustrated in fig. 3 below by Fig. 4～Fig. 8, wherein, Fig. 4 be according to p polarized light embodiment illustrated in fig. 3 reflectivity in substrate of glass/metallic film/medium system (this medium system is specifically as follows the solution in double dish 35) with the change curve schematic diagram of incident angle, Fig. 5 is the distribution schematic diagram in the surperficial x-y plane of metallic film according to the SPP light field of the surface excitation at metallic film embodiment illustrated in fig. 3, Fig. 6 is according to the distribution schematic diagram of the normal to a surface direction along metallic film embodiment illustrated in fig. 3, Fig. 7 is the distribution schematic diagram along the gradient force of x axle according to two kinds of suffered radiant forces of different medium particle embodiment illustrated in fig. 3, Fig. 8 distributes along the gradient force of z axle according to two kinds of suffered radiant forces of different medium particle embodiment illustrated in fig. 3.
What one of ordinary skill in the art will appreciate that is, according to SPP light field excitation principle, only have p light beam can excite SPP light field, for Kretchman structure, for the surface excitation SPP light field at metallic film, need to meet phase-matching condition, for specific Kretchman structure, the light beam of the specific resonant angle of needs could be at the surface excitation SPP of metallic film light wave, the refractive index of the insulating particles in the solution of this specific resonant angle and substrate of glass, metallic film and metallic film top is relevant, and relevant with the thickness of metallic film.Particularly, can determine this resonant angle through the reflectivity of Kretchman structure by calculating light beam, for example: metallic film is the golden film that 45 nanometers (nm) are thick, and metallic film is ε at the relative dielectric constant at 1064nm place
r=-5.28+2.04i; Transparent substrates is dense flint glass, refractive index n
1=1.795.As shown in Figure 4, shown respectively the refractive index n when the top medium of metallic film
31.00 (air) and n
3when=1.33 (water), the reflectivity of p polarized light is with the variation of incident angle.As shown in Figure 4, incident beam is at certain incident angle by excitating surface plasma ripple, and the reflectivity of light beam sharply declines, and has produced a resonance absorbing peak on reflectivity-incident angle curve.This curve is called surface plasma resonance (Surface Plasmon Resonance, referred to as SPR) curve, and the incident angle of corresponding minimum reflectivity is exactly surface plasmons resonant angle θ
sp.In the time that the lip-deep medium of metallic film is empty G&W, resonant angle is respectively 39.2 ° and 63.4 °.
Based on Vector Diffraction Theory the reflection in Kretchman three-decker and refraction in conjunction with light beam, can calculate the SPP optical field distribution of the surface excitation of metallic film.In the Kretchman three-decker shown in Fig. 3, if the refractive index of the solution of metallic film top is 1.33, lambda1-wavelength is 1064nm, power is 100mW, the polarization level of light beam time is P=10, the numerical aperture of oil immersion objective is 1.40, and the SPP optical field distribution of the surface excitation of metallic film as shown in Figure 5 and Figure 6.From result of calculation, SPP light field presents multifocal dot pattern, and the polarization level time P of the rotational symmetry light beam of senior time of focus quantity and incident is relevant, is specially 2 × (P-1) individual.And now each focal spot size along full width at half maximum degree (Full-width of Half-maximum radially, referred to as FWHM) be only 0.12 λ, along being also axially 0.12 λ, much smaller than Rayleigh diffraction limit size, this shows that the focal spot forming has three-dimensional super-resolution characteristic, and this kind of multifocal dot characteristics of unique three-dimensional super-resolution makes this SPP light field can catch multiple extra small particles simultaneously.
Further, if the particle of catching is spherical insulating particles, and when the radius of spherical insulating particles is during much smaller than lambda1-wavelength, can utilize the theoretical suffered radiant force of particle that calculates of Rayleigh scattering to distribute.If lambda1-wavelength is 1064nm, power is 100mW, and the radius of spheroidal particle is 50nm, and the refractive index of particle solution of living in is 1.33.The result of calculation being shown from Fig. 7 and Fig. 8, when the refractive index n of particle
pbe greater than the refractive index n of solution around
mtime, i.e. n
p> n
mtime, the gradient force that near particle focus is subject to is attractive force, and particle is attracted near focus; And when particle refractive index n
pbe less than solution refractive index n
mtime, i.e. n
p< n
mtime, the gradient force that near particle focus is subject to is repulsive force, and particle is pushed away to focus.Under normal circumstances, under the condition that meets Rayleigh scattering, the suffered gradient force of particle is all much larger than suffered scattering force.Therefore, work as n
p> n
mtime, multiple focuses that this focousing field produces will be caught multiple particles simultaneously.And because the size of focal spot is all less than Rayleigh diffraction limit, utilize these small spot can catch multiple fine particles that are less than Rayleigh diffraction limit yardstick simultaneously.
Known by above-mentioned Fig. 4～Fig. 8, can regulate and control the suffered radiant force size of particle by the power of adjusting laser instrument, thereby realize the manipulation of different scale particle.Therefore, the embodiment of the present invention, by changing flexibly polarization level time and the power of incident beam, can regulate and control flexibly the quantity of trapped particle and the particle of different scale is caught, and has larger using value.
Fig. 9 is the schematic flow sheet of an embodiment of many optical acquisitions of near field method of the present invention; Method flow in the embodiment of the present invention can be realized by the device shown in Fig. 2 and Fig. 3, and as shown in Figure 9, the embodiment of the present invention comprises the steps:
Step 901, by the first light source to polarization converter emitting parallel light bundle;
Step 902, by polarization converter, parallel beam is converted to senior time rotational symmetry light beam, and the rotational symmetry light beam of senior time is emitted to beam-expanding system;
Step 903, by beam-expanding system, senior time rotational symmetry light beam is expanded into the hot spot of setting size, and be incident to the first beam splitter by setting big or small hot spot;
Step 904, will set after the reflection of big or small hot spot by the first beam splitter, incide in the Kretchman structure being formed by oil immersion objective, substrate of glass and metallic film, wherein, substrate of glass and metallic film are placed in a double dish, comprise the solution of setting insulating particles in double dish;
Step 905, set big or small hot spot and incide and focus on the substrate of glass that is placed in double dish after oil immersion objective and the interface of metallic film forms focousing field; Wherein, set big or small hot spot and go out surface plasma-wave at the surface excitation of metallic film; Near the insulating particles light field of surface plasma-wave and metal surface interact and realize trapped particle, catch the quantity of insulating particles and are determined by the focus quantity of focousing field.
Many optical acquisitions of near field method that the embodiment of the present invention provides, the SPP light field that the rotational symmetry light beam of senior time generating by the first light source and polarization converter can comprise multiple focuses at the surface excitation of metallic film simultaneously, thus realize multiparticle optical acquisition; In addition, the polarization state of adjusting the rotational symmetry light beam of senior time by polarization converter distributes, thereby can regulate and control flexibly the SPP optical field distribution of the surface excitation of metallic film, change the focus quantity that SPP light field forms, realize and regulate and control flexibly the quantity of trapped particle and the particle of different scale is caught.
Further, on above-mentioned basis embodiment illustrated in fig. 9, can also comprise the steps:
The beam emissions that the light beam described secondary light source being sent by the second beam splitter being arranged between described oil immersion objective and described the first condenser lens leaks in described Kretchman structure is to described charge coupled cell;
By described charge coupled cell, the light beam of described leakage is carried out to imaging and obtains the shape information of described insulating particles.
Further, on above-mentioned basis embodiment illustrated in fig. 9, can also comprise the steps:
Obtain the resonant angle of the surface plasma wave resonance that the hot spot after described the first beam splitter beam splitting excites in described Kretchman structure according to the thickness of the refractive index of described substrate of glass, described metallic film and described solution and described metallic film;
Determine the field strength distribution of described surface plasma-wave according to described resonant angle.
One of ordinary skill in the art will appreciate that: all or part of step that realizes above-described embodiment can complete by the relevant hardware of programmed instruction, aforesaid program can be stored in a computer read/write memory medium, this program, in the time carrying out, is carried out the step that comprises said method embodiment; And aforesaid storage medium comprises: various media that can be program code stored such as ROM, RAM, magnetic disc or CDs.
Finally it should be noted that: above embodiment only, in order to technical scheme of the present invention to be described, is not intended to limit; Although the present invention is had been described in detail with reference to previous embodiment, those of ordinary skill in the art is to be understood that: its technical scheme that still can record aforementioned each embodiment is modified, or part technical characterictic is wherein equal to replacement; And these amendments or replacement do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (1)
1. many optical acquisitions of near field device, is characterized in that, described device comprises:
The first light source, for to polarization converter emitting parallel light bundle;
Described polarization converter, for described parallel beam being converted to the rotational symmetry light beam of senior time, and is emitted to beam-expanding system by the rotational symmetry light beam of described senior time;
Described beam-expanding system, for the rotational symmetry light beam of described senior time is expanded into the hot spot of setting size, and is incident to the first beam splitter by hot spot big or small described setting;
Described the first beam splitter is by after hot spot reflection big or small described setting, incide in the Kretchman structure being formed by oil immersion objective, substrate of glass and metallic film, described substrate of glass and described metallic film are placed in a double dish, comprise the solution of setting insulating particles in described double dish;
The big or small hot spot of described setting incides the interface that focuses on described substrate of glass and metallic film after described oil immersion objective and forms focousing field; Wherein, the big or small hot spot of described setting goes out surface plasma-wave at the surface excitation of described metallic film; The insulating particles of the light field of described surface plasma-wave and described metallic film near surface interact and realize particle-capture, described in the quantity of the insulating particles of catching determined by the focus quantity of described focousing field; Wherein, described device also comprises:
For placing the three-dimensional micro-displacement platform of described double dish, by the displacement of three-dimensional micro-displacement platform described in computer control;
Described device also comprises: secondary light source and collimation lens;
Described secondary light source is arranged on described the first beam splitter below; Described collimation lens is arranged between described the first beam splitter and described secondary light source;
The light beam that described secondary light source sends after described collimation lens collimation by described the first beam splitter, described Kretchman structured illumination to described insulating particles of catching;
Described device also comprises: the second beam splitter, charge coupled cell;
Described the second beam splitter is arranged between described oil immersion objective and described the first beam splitter; The light beam that the light beam that described secondary light source sends leaks in described Kretchman structure is emitted on described charge coupled cell through described the second beam splitter;
The light beam of described leakage is carried out imaging by described charge coupled cell, thereby obtain the shape information of described insulating particles.
2. device according to claim 1, is characterized in that, described device also comprises:
Be arranged on the condenser lens between described the second beam splitter and described charge coupled cell, for the light beam of described leakage is converged in to described charge coupled cell.
3. device according to claim 1 and 2, is characterized in that, described the first light source comprises: laser instrument, microcobjective, pin hole, collimation lens; Wherein, described laser instrument is used for sending light beam, and described light beam is parallel beam by described collimation lens collimation after described microcobjective and described pinhole filter expand.
4. device according to claim 1 and 2, it is characterized in that, the resonant angle of the hot spot excitating surface plasma wave resonance in described Kretchman structure after described the first beam splitter beam splitting is determined by the refractive index of described substrate of glass, described metallic film and described solution and the thickness of described metallic film.
5. many optical acquisitions of near field method, is characterized in that, described method comprises:
By the first light source to polarization converter emitting parallel light bundle;
By described polarization converter, described parallel beam is converted to the rotational symmetry light beam of senior time, and the rotational symmetry light beam of described senior time is emitted to beam-expanding system;
By beam-expanding system, the rotational symmetry light beam of described senior time is expanded into the hot spot of setting size, and hot spot big or small described setting is incident to the first beam splitter;
After hot spot big or small described setting being reflected by described the first beam splitter, incide in the Kretchman structure being formed by oil immersion objective, substrate of glass and metallic film, described substrate of glass and described metallic film are placed in a double dish, comprise the solution of setting insulating particles in described double dish;
The big or small hot spot of described setting incides and focuses on the substrate of glass that is placed in double dish after described oil immersion objective and the interface of metallic film forms focousing field; Wherein, the big or small hot spot of described setting goes out surface plasma-wave at the surface excitation of described metallic film; The insulating particles of the light field of described surface plasma-wave and described metallic film near surface interact and realize particle-capture, described in the quantity of the insulating particles of catching determined by the focus quantity of described focousing field; Wherein,
Described method also comprises:
The beam emissions that light beam secondary light source being sent by the second beam splitter being arranged between described oil immersion objective and the first condenser lens leaks in described Kretchman structure is to charge coupled cell;
By described charge coupled cell, the light beam of described leakage is carried out to imaging and obtains the shape information of described insulating particles.
6. method according to claim 5, is characterized in that, described method also comprises:
Obtain the resonant angle of the surface plasma wave resonance exciting after described the first beam splitter beam splitting in described Kretchman structure according to the thickness of the refractive index of described substrate of glass, described metallic film and described solution and described metallic film;
Determine the field strength distribution of described surface plasma-wave according to described resonant angle.
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| CN104900291B (en) * | 2015-04-23 | 2017-03-15 | 深圳大学 | A kind of SPP optical optical tweezers systems |
| CN105588954B (en) * | 2016-03-23 | 2018-06-29 | 上海理工大学 | Near field polarised light scanning probe microscopy |
| CN107515047B (en) * | 2017-09-20 | 2019-08-30 | 鲁东大学 | Detect the device and method of sub-wavelength dimensions multifocal hot spot polarization orientation |
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| "Optical trapping of micrometer-sized dielectric particles by cylindrical vector beams";Yuichi Kozawa et al;《OPTICS EXPRESS》;20100510;第18卷(第10期);参见期刊第10829页第3段至第10832段第2段、附图1-2 * |
| "远场光钳与近场光钳的发展";胡朝晖;《光学技术》;20030531;第29卷(第3期);第267页第2段、图2 * |
| Yuichi Kozawa et al."Optical trapping of micrometer-sized dielectric particles by cylindrical vector beams".《OPTICS EXPRESS》.2010,第18卷(第10期),参见期刊第10829页第3段至第10832段第2段、附图1-2. |
| 周哲海.轴对称偏振光束的生成、特性及应用.《中国博士学位论文全文数据库 基础科学辑》.2012,第97页第2段至第104页倒数第1段、图5.5. |
| 胡朝晖."远场光钳与近场光钳的发展".《光学技术》.2003,第29卷(第3期),第267页第2段、图2. |
| 轴对称偏振光束的生成、特性及应用;周哲海;《中国博士学位论文全文数据库 基础科学辑》;20120314;第97页第2段至第104页倒数第1段、图5.5 * |
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