CN102645358A - Preparation method of water-soluble cholesterol lyophilized product derived from serum - Google Patents
Preparation method of water-soluble cholesterol lyophilized product derived from serum Download PDFInfo
- Publication number
- CN102645358A CN102645358A CN2012100996386A CN201210099638A CN102645358A CN 102645358 A CN102645358 A CN 102645358A CN 2012100996386 A CN2012100996386 A CN 2012100996386A CN 201210099638 A CN201210099638 A CN 201210099638A CN 102645358 A CN102645358 A CN 102645358A
- Authority
- CN
- China
- Prior art keywords
- serum
- cholesterol
- preparation
- water
- aquatic products
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to a cholesterol lyophilized product, and in particular relates to a preparation method of a water-soluble cholesterol lyophilized product derived from serum. The preparation method comprises the following steps of: adjusting pH value of the serum to 6.9 by utilizing 1mol/L of sodium hydroxide, taking 150ml of absolute ethyl alcohol for dropwise adding to the continuously stirred serum, stirring the serum for 2h at the room temperature, centrifuging the serum for 10min per 1000g and collecting precipitates; weighing 0.3g of glucan and 6.5g of calcium chloride, dissolving the glucan and the calcium chloride to 500ml of deionized water, dissolving the precipitates collected from the former step by the solution, stirring the solution for 2h at the room temperature, centrifuging the solution for 10min per 1000g and collecting the precipitates again; and redissolving the collected precipitates and lyophilizing the precipitates to obtain a cholesterol- rich lyophilized product. Compared with the prior art, with the adoption of the preparation method, cholesterol is lyophilized and then is redissolved by the deionized water, the appearance is clear and bright, the purity of the cholesterol is high, the repeatability is good, and the recovery rate is high.
Description
[technical field]
The present invention relates to a kind of dried frozen aquatic products of cholesterol, a kind of specifically preparation method who is applied in the water-soluble cholesterol dried frozen aquatic products that derives from serum of biology, medical test research.
[background technology]
Cholesterol, the another name cholesterol, chemical formula is C
27H
46O is a kind of steroids and sterol.Cholesterol is water-soluble very poor organic compound, and the solubleness of pure article cholesterol in water approximately has only 0.095mg/L (30 ℃), therefore under low like this dissolubility, will be difficult to add as solution preparation or calibration object/quality-control product preparation.Contain certain density water-soluble cholesterol owing to have in the serum, these water-soluble cholesterol or combine with some compound, or combine with lipoprotein; Thereby have stronger water-solublely, Given this, some enterprise takes high concentration serum to be used as preparation to use at present; Also have some through concentrating the cholesterol concentrate that serum obtains high concentration; But the defective of these ways is: be difficult to obtain the purity high product first, its serum matrix content is very high, thereby has influenced the quality of product; Once more, owing to be fluid product, its less stable.
Keep bioactive material for dry thermal sensitivity goods with needing, freeze-drying is a kind of effective method.This method has prevented the change of goods physics and chemistry and biological nature effectively, can effectively protect the stability of many thermal sensitivity medicine biological products effective ingredients.Up to the present there are employing freeze drying process such as a lot of medicines, biological products to prepare.
[summary of the invention]
The present invention provides a kind of preparation method who derives from the water-soluble cholesterol dried frozen aquatic products of serum in order to overcome the deficiency of prior art.
To achieve these goals, the present invention has designed a kind of preparation method who derives from the water-soluble cholesterol dried frozen aquatic products of serum, adopts following steps:
A, its pH value is transferred to 6.9, get in the serum that the 150ml absolute ethyl alcohol dropwise adds continuous stirring with the NaOH of 1mol/L, behind the stirring at room 2h, the centrifugal 10min of 1000g, collecting precipitation thing;
B, take by weighing 0.3g glucosan and 6.5g lime chloride, be dissolved in the 500ml deionized water, with the deposition of collecting among this solution dissolving step A, behind the stirring at room 2h, the centrifugal 10min of 1000g, collecting precipitation thing once more;
C, the sediment of collecting among the step B is dissolved again, freeze-drying afterwards promptly gets the dried frozen aquatic products that is rich in cholesterol.
In the said steps A, the content of serum is 1L, and pH is 5-9.
Among the said step B, the concentration of lime chloride is 25mmol/L.
Among the said step B, the concentration of glucosan is 2.6mmol/L.
The present invention compares with prior art, redissolves with deionized water after the cholesterol freeze-drying, and it is clear and bright that outward appearance is, and cholesterol purity is high, and this dried frozen aquatic products can steady in a long-termly be stored, easy and simple to handle, the good reproducibility of method, and the recovery is high.And it derives from serum, therefore can be widely used in fields such as biology, medical test.
[embodiment]
The invention provides the dried frozen aquatic products that is rich in water-soluble cholesterol in a kind of serum source.Dried frozen aquatic products good stability provided by the invention, the back liquid that redissolves is clear and bright, does not contain foreign matter or insolubles, water miscible cholesterol neither can have tangible interference to the detection of conventional project, can be to other components yet stable influential.
Through embodiment the present invention is further described below.
The present invention is a kind of preparation method who derives from the water-soluble cholesterol dried frozen aquatic products of serum; Adopt following steps: be example (A) with 1L serum; NaOH with 1mol/L transfers to 6.9 with its pH value, gets in the serum that the 150ml absolute ethyl alcohol dropwise adds continuous stirring, behind the stirring at room 2h; The centrifugal 10min of 1000g, the collecting precipitation thing;
(B) take by weighing 0.3g glucosan and 6.5g lime chloride, be dissolved in the 500ml deionized water, with the deposition of collecting among this solution dissolving step A, behind the stirring at room 2h, the centrifugal 10min of 1000g, collecting precipitation thing once more;
(C) sediment of collecting among the step B is dissolved again, freeze-drying afterwards promptly gets the dried frozen aquatic products that is rich in cholesterol.
Experiment one, the visual testing of redissolving the back cholesterol
After will distinguishing serial number by the dried frozen aquatic products of the foregoing description preparation, adopt the number of table of random numbers decision draw samples, randomly draw 5 and do check.Add deionized water and redissolve, dried frozen aquatic products dissolves fast, and the 10-15min dissolving is complete, and liquid is clear and bright, does not contain foreign matter or insolubles.
The mensuration of experiment two, cholesterol concentration
The cholesterol enzymatic assays is easy, has enough sensitivity, and precision is good, so select the enzymatic assays cholesterol concentration for use.Select the cholesterol reagent box for use, adopt automatic biochemistry analyzer that the cholesterol dried frozen aquatic products of 5 redissolution is measured, concrete parameter sees table.
Can know that through last table the content of cholesterol dried frozen aquatic products is very high.
Experiment three, cholesterol dried frozen aquatic products STABILITY MONITORING
The cholesterol dried frozen aquatic products is placed on 2-8 ℃ of refrigerator carries out the long-time stability experiment, with the concentration of cholesterol reagent box cholesterol detection, calculate relative deviation after the preservation different time, concrete data see table.
Above-mentioned data show that dried frozen aquatic products has good stability.
Claims (4)
1. preparation method who derives from the water-soluble cholesterol dried frozen aquatic products of serum, adopt following steps:
(A) NaOH with 1mol/L transfers to 6.9 with its pH value, get in the serum that the 150ml absolute ethyl alcohol dropwise adds continuous stirring, and behind the stirring at room 2h, the centrifugal 10min of 1000g, collecting precipitation thing;
(B) take by weighing 0.3g glucosan and 6.5g lime chloride, be dissolved in the 500ml deionized water, with the deposition of collecting among this solution dissolving step A, behind the stirring at room 2h, the centrifugal 10min of 1000g, collecting precipitation thing once more;
(C) sediment of collecting among the step B is dissolved again, freeze-drying afterwards promptly gets the dried frozen aquatic products that is rich in cholesterol.
2. the preparation method who derives from the water-soluble cholesterol dried frozen aquatic products of serum according to claim 1 is characterized in that: in the said steps A, the content of serum is 1L, and pH is 5-9.
3. the preparation method who derives from the water-soluble cholesterol dried frozen aquatic products of serum according to claim 1 is characterized in that: among the said step B, the concentration of lime chloride is 25mmol/L.
4. the preparation method who derives from the water-soluble cholesterol dried frozen aquatic products of serum according to claim 1 is characterized in that: among the said step B, the concentration of glucosan is 2.6mmol/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210099638.6A CN102645358B (en) | 2012-04-06 | 2012-04-06 | Preparation method of water-soluble cholesterol lyophilized product derived from serum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210099638.6A CN102645358B (en) | 2012-04-06 | 2012-04-06 | Preparation method of water-soluble cholesterol lyophilized product derived from serum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102645358A true CN102645358A (en) | 2012-08-22 |
| CN102645358B CN102645358B (en) | 2014-09-10 |
Family
ID=46658320
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210099638.6A Active CN102645358B (en) | 2012-04-06 | 2012-04-06 | Preparation method of water-soluble cholesterol lyophilized product derived from serum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102645358B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11604026B2 (en) | 2019-03-14 | 2023-03-14 | Terumo Bct Biotechnologies, Llc | Lyophilization loading tray assembly and system |
| US11634257B2 (en) | 2017-10-09 | 2023-04-25 | Terumo Bct Biotechnologies, Llc | Lyophilization container and method of using same |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101643495A (en) * | 2009-07-23 | 2010-02-10 | 浙江赞宇科技股份有限公司 | Method for extracting plant sterol from fatty acid or methyl ester distilled black foot thereof |
-
2012
- 2012-04-06 CN CN201210099638.6A patent/CN102645358B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101643495A (en) * | 2009-07-23 | 2010-02-10 | 浙江赞宇科技股份有限公司 | Method for extracting plant sterol from fatty acid or methyl ester distilled black foot thereof |
Non-Patent Citations (4)
| Title |
|---|
| 张旭等: "蛋黄中胆固醇含量测定方法的研究", 《饲料博览》 * |
| 张秀明等: "改良荧光法测定血清水溶性脂质过氧化物", 《陕西医学检验》 * |
| 王健等: "冷冻干燥对提高脂质体稳定性的研究概况", 《中国医药工业杂质》 * |
| 黄启平: "从羊毛脂中提取胆固醇的研究", 《精细化工》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11634257B2 (en) | 2017-10-09 | 2023-04-25 | Terumo Bct Biotechnologies, Llc | Lyophilization container and method of using same |
| US11604026B2 (en) | 2019-03-14 | 2023-03-14 | Terumo Bct Biotechnologies, Llc | Lyophilization loading tray assembly and system |
| US11609043B2 (en) | 2019-03-14 | 2023-03-21 | Terumo Bct Biotechnologies, Llc | Lyophilization container fill fixture, system and method of use |
| US11609042B2 (en) | 2019-03-14 | 2023-03-21 | Terumo Bct Biotechnologies, Llc | Multi-part lyophilization container and method of use |
| US11740019B2 (en) | 2019-03-14 | 2023-08-29 | Terumo Bct Biotechnologies, Llc | Lyophilization loading tray assembly and system |
| US11747082B2 (en) | 2019-03-14 | 2023-09-05 | Terumo Bct Biotechnologies, Llc | Multi-part lyophilization container and method of use |
| US11815311B2 (en) | 2019-03-14 | 2023-11-14 | Terumo Bct Biotechnologies, Llc | Lyophilization container fill fixture, system and method of use |
| US11994343B2 (en) | 2019-03-14 | 2024-05-28 | Terumo Bct Biotechnologies, Llc | Multi-part lyophilization container and method of use |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102645358B (en) | 2014-09-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103789202A (en) | Container for collecting nucleic acid preserved at normal temperature | |
| CN105349529A (en) | Freeze-drying protective agent applied to nucleic acid amplification system | |
| CN105652022A (en) | Thromboelastography quality control material and preparation method thereof | |
| CN102645358A (en) | Preparation method of water-soluble cholesterol lyophilized product derived from serum | |
| BR112019021396A2 (en) | pullulan manufacturing process | |
| JP6230796B2 (en) | Freeze-dried bacteria sample and production method thereof | |
| CN108285925A (en) | A kind of rugged Cronobacter sakazakii quick detection kit of slope | |
| CN105274187A (en) | Shigella standard substance containing chicken matrix | |
| Wu et al. | Sampling methods for NMR‐based metabolomics of Staphylococcus aureus | |
| CN102771472A (en) | Freezing liquid for preserving embryo, preparation method and application thereof | |
| CN104181313A (en) | Preparation method of blood coagulation factor IX quality control product | |
| CN104569446A (en) | Liquid-type prothrombin time detection reagent and preparation method thereof | |
| CN104360052B (en) | A kind of Clenbuterol detection Quality Control animals urine dry powder and preparation method and application | |
| CN104726533A (en) | Cold-water gellable medium coagulating agent, preparation method and application thereof | |
| CN106350499B (en) | The stabilizer of thrombin solution | |
| CN107988327A (en) | The dry powdered LAMP quick detection kits of Shigella | |
| CN104166004B (en) | Platelet cofactor Ⅰ quality-control product preparation method | |
| CN106350500B (en) | Thrombin solution | |
| CN104698159B (en) | A kind of detection method of endotoxin content | |
| Cheng et al. | Study on electrochemical fingerprints of different Chrysanthemums by using B–Z oscillation system | |
| CN115160450B (en) | Rapid preparation method and application of Pholiota nameko polysaccharide | |
| Raghunathan et al. | Trial by fire: are the crystals macromolecules? | |
| Li et al. | Mechanistic insights into the improvement of yeast viability by adding short-clustered maltodextrin during long-term frozen storage | |
| CN102183512A (en) | Living cell detection method based on chemiluminescence principle | |
| CN105647785B (en) | A kind of Blood culture bottle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 201100 Shanghai East Road, Minhang District, No. 85 Patentee after: SHANGHAI AILEX TECHNOLOGY Co.,Ltd. Address before: 201100 Shanghai East Road, Minhang District, No. 85 Patentee before: SHANGHAI AILEX TECHNOLOGY Co.,Ltd. |
|
| CP03 | Change of name, title or address |
Address after: 201108 Lane 152, 468, Beihengsha River Road, Minhang District, Shanghai Patentee after: AILEX TECHNOLOGY GROUP Co.,Ltd. Address before: No. 85 Youdong Road, Minhang District, Shanghai 201100 Patentee before: SHANGHAI AILEX TECHNOLOGY Co.,Ltd. |
|
| CP03 | Change of name, title or address | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20181211 Address after: 201108 Lane 152, 468, Beihengsha River Road, Minhang District, Shanghai Co-patentee after: ZHEJIANG AILEX MEDICAL Co.,Ltd. Patentee after: AILEX TECHNOLOGY GROUP Co.,Ltd. Address before: 201108 Lane 152, 468, Beihengsha River Road, Minhang District, Shanghai Patentee before: AILEX TECHNOLOGY GROUP Co.,Ltd. |
|
| TR01 | Transfer of patent right |