CN102641284A - Application of spirulina platensis polysaccharide - Google Patents
Application of spirulina platensis polysaccharide Download PDFInfo
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- CN102641284A CN102641284A CN2012101343531A CN201210134353A CN102641284A CN 102641284 A CN102641284 A CN 102641284A CN 2012101343531 A CN2012101343531 A CN 2012101343531A CN 201210134353 A CN201210134353 A CN 201210134353A CN 102641284 A CN102641284 A CN 102641284A
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Abstract
The invention relates to application of spirulina platensis polysaccharide, which is applied to preparing preparations for restraining tumor growth, preparations with antioxygenation functions to cells and nuclear factor kappa (NF-KB) immune preparations. A spirulina platensis washing gavage administration method has restraining functions on S180 tumors. After administration, spirulina platensis water is used for extracting washing polysaccharide of 100mg/kg, and T/C% is 58.95%. Compared with a control group, the weight of a tumor is obviously different (rho (0.05). Spleen cells of a mice immune organ are used for multiplication experiments of immune cells. Results show that 40, 200 and 1000 ug/ml of spirulina platensis polysaccharide all have functions of promoting multiplication of spleen cells, and when the T/C% is respectively 148.96%, 166.89% and 182.99%, and the T/C% is obviously different from a medicine-free group (rho (0.05). 0.2mol/l of spirulina platensis, 8ug/ml of Nacl elution polysaccharide and 40ug/ml of the Nacl elution polysaccharide have effects on promoting multiplication of spleen cells, and when the T/C% is respectively 19.88% and 202.81%, the T/C% is obviously different from the medicine-free group (rho (0.05).
Description
Technical field
The present invention relates to a kind of application of spirulina polysaccharide.
Background technology
Spirulina is one type of rudimentary plant, belongs to Cyanophyta, Oscillariaceae.They are the same with antibacterial, do not have real nucleus in the cell, so claim cyanobacteria again.The cellularity of cyanophyceae is original, and very simple, and it grows in the water body, is that spiral is thread in visible its form of microscopically.The existing report of relevant spirulina polysaccharide anti-tumor activity research, but majority is a crude polysaccharides, anti-tumor activity is to derive from which kind of polysaccharide, its clear and definite structure is how, does not appear in the newspapers.
Summary of the invention
The purpose of this invention is to provide a kind of application of spirulina polysaccharide.
Spirulina polysaccharide of the present invention is used to prepare the preparation that suppresses tumor growth.
Spirulina polysaccharide of the present invention is used to prepare the preparation of pair cell Wheat Protein.
Spirulina polysaccharide of the present invention is used to prepare the NF-KB immune formulation.
Spirulina polysaccharide of the present invention is to the experiment of S180 murine sarcoma, and the water of the oral spirulina polysaccharide of discovery mice is carried with alkali and proposed the effect that part all has part inhibition tumor growth.Further experiment; Washing, 0.2,0.3,0.5 mol/l Nacl eluting that water is carried polysaccharide carry out the research of proliferation experiment to mouse boosting cell; Find that water proposes the effect that the water elution of polysaccharide partly has stronger promotion splenocyte propagation, verify the inhibitory action of its component more in vivo the S180 sarcoma.The result shows that spirulina washing gastric infusion mode has inhibitory action to the S180 tumor, and when administration finished, the T/C% that spirulina water water lift is washed polysaccharide 100mg/kg was 58.95%, relatively there were significant differences for tumor weight and matched group (
p<0.05).
Carry out the proliferation experiment of immunocyte with the splenocyte of mouse immune organ; The result shows: spirulina washing polysaccharide 40,200,1000ug/ml all have the promotion proliferation function to splenocyte, T/C% be respectively 148.96%, 166.89%, 182.99% and not dosing group there were significant differences (
p<0.05).Spirulina 0.2moll Nacl eluting polysaccharide 8ug/ml and 40ug/ml to splenocyte also have promote increment effect, T/C% be respectively 179.88% and 202.81% and not dosing group there were significant differences (
p<0.05).
With cell under environmental stimuli thing effects such as endotoxin LPS; LPS is through after being attached to the TLR4 receptor; Cause through MyD88 that rely on two pathway activation NF-κ B TRIF, receive the reagent thing during experiment simultaneously, detect the activation that under drug effect, whether suppresses NF-κ B.Simultaneously do not give environmental stimuli, give drug treating separately, detect and under drug effect, whether activate NF-κ B.As a result, spirulina water water lift washes 40,200, the 1000ug/ml dose groups all has inhibition to NF-κ B, and suppression ratio is respectively: 99.9%, 97.6% and 92.0%.
Induce PC12 cell injury simulation gerontal patient because the deterioration of neurons that the excessive accumulation of ROS causes is studied the anti-aging effects of polysaccharide and possible mechanism thereof with hydrogen peroxide.As a result, spirulina polysaccharide four components all to PC12 cell Wheat Protein, more all have significant difference with the H2O2-400uM group 40, in the 200ug/ml.
Description of drawings
Fig. 1. spirulina polysaccharide is to the tumor multigraph of S180 transplanted tumor.
Fig. 2. spirulina polysaccharide is to the growth curve chart of the weight of animals of S180 mice transplantation model.
Fig. 3. the tumor photo.
Fig. 4. spirulina water is carried the tumor multigraph of polysaccharide water elution polysaccharide component to S180 transplanted tumor.
Fig. 5. spirulina water is carried the growth curve chart of polysaccharide water elution polysaccharide component to the weight of animals of S180 mice transplantation model.
Fig. 6. the tumor photo.
Fig. 7. the spirulina purified polysaccharide is to mouse boosting cell increment experiment (T/C%).
Fig. 8. each is organized medicine and compares without the hydrogen peroxide processed group.
Fig. 9. each organizes the suppression ratio figure of sample to NF-κ B.
The specific embodiment
Embodiment 1:
Spirulina polysaccharide of the present invention is used to prepare the preparation that suppresses tumor growth.
The concrete experiment as follows:
One, the anti-S180 murine sarcoma experiment of crude polysaccharides
1. test material and method
1.1 given the test agent: spirulina water is carried polysaccharide, brown dry powder, and exsiccator keeps in Dark Place.The spirulina alkali-extracted polysaccharide, light green color dry powder, exsiccator keeps in Dark Place.
Supply the test agent precision weighing, in the aseptic workbench drug powder is mixed with maximum dose level, gradient dilution is divided in 4 degree preservations in the centrifuge tube, the administration of taking a sample every day to low dosage again.
1.2 experimental animal: the ICR mice, female 6 ages in week, 36, available from Si Laike Animal resources center, Shanghai.
1.3 dosage divides into groups
The test dose grouping is provided with and sees the following form:
| Group | Compound | Animals | Route | Dose (mg/kg) | |
| 1 | Control (Saline) | 6 | Po | 0.1ml/10g, BW | Qd*9 |
| 2 | Spirulina- | 6 | Po | 200mg/kg | Qd*9 |
| 3 | Spirulina- | 6 | Po | 500mg/kg | Qd*9 |
| 4 | Spirulina-OH | 6 | Po | 200mg/kg | Qd*9 |
| 5 | Spirulina- | 6 | Po | 500mg/kg | Qd*9 |
| 6 | | 6 | Ip | 30mg/kg | Qd*9 |
1.4 test method: the foundation of transplantation model: the S180 cell goes down to posterity through the ICR mouse ascites, get ascites 1:5 dilution during experiment after, be seeded to the right side of mice axil with 0.2ml/mouse, inoculate second day and begins administration, successive administration 9 days.
Duration of test is measured the weight of animals weekly twice.Every day the observed and recorded clinical symptoms.When administration finishes, get tumor and weigh.The evaluation index of anti-tumor activity is relative tumor appreciation rate T/C (%), and computing formula is: T/C (%)=(T/C) ' 100%.T is that treatment group tumor is heavy, and the negative matched group tumor of C is heavy.
Therapeutic evaluation standard: T/C (%) ￡ 60% and through statistical analysis p < 0.05 is effective.
2. result: spirulina water bill of lading dosage 200 and 500 mg/kg, spirulina alkali bill of lading dosage 200 and 500 mg/kg, every day, gastric infusion was continuous 9 days, carried out the pharmacodynamics test of S180 mice transplantation model.The result shows that this extract gastric infusion mode has partial inhibition to the S180 tumor.When administration finished, the T/C% that spirulina water is carried polysaccharide was respectively 58.50% and 44.66%.The T/C% of spirulina alkali-extracted polysaccharide is respectively 48.79% and 47.94%.Each organizes dosage T/C%, and < relatively there were significant differences for 60% tumor weight and matched group (p < 0.05), but SD is excessive, treats further research.Do not see the xicity related reaction of other drug in the process of the test.
Spirulina polysaccharide is to the inhibitory action of S180 transplanted tumor
| Tumor (g) | T/C (%) | |
| Control (saline) | 1.37 ± 0.20 | |
| Spirulina-H2O 200mg/kg, po | 0.80 ± 0.52* | 58.50 |
| Spirulina-H2O 500mg/kg, po | 0.61 ± 0.58* | 44.66 |
| Spirulina-OH 200mg/kg, po | 0.67 ± 0.31* | 48.79 |
| Spirulina-OH 500mg/kg, po | 0.66 ± 0.57* | 47.94 |
| CTX 30mg/kg, ip | 0.30 ± 0.10** | 21.72 |
*：p<0.05,?compare?with?control?group;?**：p<0.01,?compare?with?control?group.
Two, the anti-S180 murine sarcoma experiment of purified polysaccharide
1. test material and method
1.1 given the test agent: spirulina water is carried polysaccharide water elution polysaccharide component, and white dry powder is kept in Dark Place by exsiccator.Supply the test agent precision weighing, in the aseptic workbench drug powder is mixed with maximum dose level, gradient dilution is divided in-20 degree preservations in the centrifuge tube, the administration of taking a sample every day to low dosage again.
1.2 experimental animal: the ICR mice, female 6 ages in week, 40, available from Si Laike Animal resources center, Shanghai.
1.3 dosage divides into groups
The test dose grouping is provided with and sees the following form:
| Group | Compound | Animals | Route | Dose (mg/kg) | |
| 1 | Control (Saline) | 8 | Po | 0.1ml/10g, BW | Qd*10 |
| 2 | Spirulina-1 | 8 | Po | 30mg/kg | Qd*10 |
| 3 | Spirulina-1 | 8 | Po | 100mg/kg | Qd*10 |
| 4 | Spirulina-1 | 8 | Po | 300mg/kg | Qd*10 |
| 5 | | 8 | Ip | 30mg/kg | Qd*10 |
1.4 test method: the foundation of transplantation model: the S180 cell goes down to posterity through the ICR mouse ascites, get ascites 1:5 dilution during experiment after, be seeded to the right side of mice axil with 0.2ml/mouse, inoculate second day and begins administration, successive administration 10 days.
Duration of test is measured the weight of animals weekly twice.Every day the observed and recorded clinical symptoms.When administration finishes, get tumor and spleen is weighed.The evaluation index of anti-tumor activity is relative tumor appreciation rate T/C (%), and computing formula is: T/C (%)=(T/C) ' 100%.T is that treatment group tumor is heavy, and the negative matched group tumor of C is heavy.
Therapeutic evaluation standard: T/C (%) ￡ 60% and through statistical analysis p < 0.05 is effective.
2. result: spirulina water is carried polysaccharide water elution polysaccharide component single dose 30,100 and 300 mg/kg, and every day, gastric infusion was continuous 10 days, carried out the pharmacodynamics test of S180 mice transplantation model.The result shows that this extract gastric infusion mode has inhibitory action to the S180 tumor.When administration finished, the T/C% that spirulina water water lift is washed polysaccharide 100 mg/kg was 58.95%, relatively there were significant differences for tumor weight and matched group (
p<0.05).Do not see the xicity related reaction of other drug in the process of the test.
Spirulina water is put forward the inhibitory action of polysaccharide water elution polysaccharide component to S180 transplanted tumor
| Tumor (g) | Spleen (g) | T/C (%) | |
| Control | 1.19 ± 0.39 | 0.23 ± 0.07 | |
| Spirulina-1 30mg/kg, PO | 1.49 ± 0.30 | 0.23 ± 0.05 | 125.26 |
| Spirulina-1 100mg/kg, PO | 0.70 ± 0.25* | 0.20 ± 0.00 | 58.95 |
| Spirulina-1 300mg/kg, PO | 1.50 ± 0.32 | 0.21 ± 0.06 | 126.32 |
| CTX 30mg/kg, ip | 0.81 ± 0.27* | 0.19 ± 0.04 | 68.42 |
*：p<0.05,?compare?with?control?group;?**：p<0.01,?compare?with?control?group。
Embodiment 2:
Spirulina polysaccharide is used to prepare the preparation of pair cell Wheat Protein.
One, polysaccharide is to the mouse boosting cell proliferation experiment
1. test material and method
1.1 cell and sample are prepared
Get 6-8 mice in age in week, cervical vertebra dislocation mice is got spleen rapidly, is placed in the cold HBSS of a dish and makes its ice bath as early as possible, removes epithelial cell and fatty tissue, grinds 100 mesh screens, centrifugal 250 g, 5 minutes.Abandoning supernatant is used hyposmosis Gey ' s (0.15 M NH4Cl, 10 mM KHCO
3) solution eliminates erythrocyte, after about 2 minutes, with the cold HBSS inferior cell of giving a baby a bath on the third day after its birth.RPMI-the RPMI-1640 that adds 10% hyclone, the adjustment concentration of cell suspension is to (1 * 10
7Cells/ml), put into 96 holes then and cultivate version 100 μ L/ holes (1 * 10
6Cells/well), add concanavalin A, Con A (C of Sigma company-2010,5 mcg/ml) then, set up blank with 100 μ l culture medium simultaneously.All samples is triplicate.Will be under 37 ℃ of conditions, 5% CO2 gas incubator was cultivated 48 hours.
The polysaccharide sample: the spirulina crude polysaccharides is through the DEAE resin column, with distilled water, 0.2,0.3,0.5 mol/L NaCl eluting gained polysaccharide component successively.
1.2 detect: the BrdU quantification kit (Luo Shi #11647229001) that uses ELISA method on cell proliferation.Add BrdU labelling 20 μ L/ holes, cultivated again again 6 hours or spend the night.Centrifugal 300 g 10 minutes, eject culture fluid, at 60 ℃ of drying 1 h then fast.With reference to BrdU test kit description, add FixDenat, anti-BrdU-POD, substrate successively up to the suitable photometric detection of color.Add 25 μ l/well, 2 N H
2SO
4Stop buffer, the absorbance of 5 minutes inherent 450 witness mark 690 nm of nm place.
OD?Test?-?OD?blank
T/C?(%)?=
*100
OD?Control-OD?blank
2. result: carry polysaccharide component with distilled water, 0.2,0.3,0.5 mol/L NaCl eluting with spirulina water, obtain four components and carry out the research of mouse boosting cell proliferation experiment.The result shows: spirulina washing polysaccharide 40,200,1000 μ g/ml all have the promotion proliferation function to splenocyte, T/C% be respectively 148.96%, 166.89%, 182.99% and not dosing group there were significant differences (
p<0.05).Spirulina 0.2moll Nacl eluting polysaccharide 8ug/ml and 40ug/ml to splenocyte also have promote increment effect, T/C% be respectively 179.88% and 202.81% and not dosing group there were significant differences (
p<0.05).
The spirulina purified polysaccharide is to mouse boosting cell increment experiment (T/C%)
*：
p?<?0.05,?compare?with?control?group;?**：
p?<?0.01,?compare?with?control?group
Two, hydrogen peroxide is induced nerve cell death protective effect research
Hydrogen peroxide (H
2O
2) be that active chalcogen (can stride film and diffuse in the cell, is a kind of cellular oxidation stress-induced agent relatively more commonly used, is widely used in the cell death inducing model by reac2tiveoxygenspecies, one of main component ROS).Thereby PC12 cell injury simulation gerontal patient induced by this research and utilization hydrogen peroxide because the deterioration of neurons that the excessive accumulation of ROS causes is studied the anti-aging effects of polysaccharide and possible mechanism thereof.
1. material
1.1 the configuration of polysaccharide: spirulina water is carried crude polysaccharides through DEAE post water successively; 0.2; 0.3 and 0.5 M NaCl eluting polysaccharide component, four components are configured to the storage liquid of 10 mg/ml with PBS;-20 degree are preserved, and the working concentration that is diluted to 1 mg/ml, 200 μ g/ml, 40 μ g/ml with culture medium during experiment carries out drug treating.
1.2 reagent: CCK-8 (Japanese colleague's chemistry institute); The DMEM culture medium (HyClone, Thermo scientific, USA); Horse serum; Newborn calf serum; 30% hydrogen peroxide
1.3 instrument: CO2 cell culture incubator; Inverted microscope; Analytical balance; High-speed refrigerated centrifuge; The long ELIASA of all-wave
2. method
2.1 PC12 cell culture and processing: the PC12 cell strain is provided by ATCC, is stored in the liquid nitrogen, with the DMEM culture medium culturing that contains 10 % horse serums, 5 % newborn calf serums, 100 U/mL penicillins, 100 μ g/mL streptomycins.Place 37 ° of C saturated humidities, contain 5% CO
2With cultivate in the calorstat of 95% air, directly piping and druming need not trypsinization and goes down to posterity, and treats to make an experiment when cell reached for 10～20 generations.
2.2 H
2O
2Handle: the PC12 cell is with 1 * 10
4The density of cells/well is inoculated in 96 orifice plates; Give the spirulina different component behind adherent 24 h and handle, concentration is respectively 40 μ g/ml, 200 μ g/ml and 1 mg/ml, gives 400 μ M hydrogen peroxide treatment behind administration 20 min; Omnidistance administration during the processing, blank group are relatively.
2.3 WST colorimetric analysis: behind hydrogen peroxide treatment 4 h, every hole adds 20 μ l CCK-8,37 ℃ hatch 2 h after, 450 nm wavelength are measured absorbance on the ELIASA.
2.4 statistical procedures: adopt the SPSS statistical software to carry out statistical procedures.Data all with mean ± standard deviation (
x±
s) expression, relatively adopt independent sample between two groups
tCheck is relatively adopted one factor analysis of variance between many groups.
3. result
Each is organized medicine and compares without the hydrogen peroxide processed group
*：p<0.05,?compare?with?H2O2-400uM;?**：p<0.01,?compare?with?H2O2-400uM.
Embodiment 3:
Spirulina polysaccharide is used to prepare the NF-KB immune formulation.Test as follows:
The screening of spirulina polysaccharide targeting NF-κ B signal path immunocompetence
1. material
1.1 medicine configuration: with spirulina water carry the crude polysaccharides component through the DEAE resin column with distilled water, 0.2,0.3,0.5 mol/L NaCl solution eluting successively, obtain four components and be directed against NF-κ B immunosuppressive activity and screen.Sample is mixed with 10 mg/ml storage liquid with PBS respectively, and-20 degree are preserved, and the experiment thing is carried out drug treating with the working concentration that culture medium is diluted to 40 μ g/ml, 200 μ g/ml, 1000 μ g/ml.
1.2 reagent: LPS (Sigma, L6529); CCLR (Reporter Lysis Buffer, Promega, 28303701); Luciferin (Promega, E4550)
1.3 instrument: analytical balance; The long ELIASA of all-wave; High speed centrifuge
2. method
2.1 cell culture: 293 cells are that people's renal epithelial cell system is bought by Chinese Academy of Sciences's Shanghai school of life and health sciences cell bank; Be stored in the liquid nitrogen; 293/NF-κ B-Luc cell detects the activation degree of NF-κ B with the NF-kB-luc reporter gene, cultivates with the DMEM high glucose medium that contains 10 % hyclones (Gibco company).Place 37 ℃ of saturated humidities, contain 5%CO
2With cultivate in the calorstat of 95% air, 0.25% trypsinization goes down to posterity.
2.2 detect
1) collecting cell 293/ NF-κ B-Luc is with 5 * 10
5The cell concentration of/ml is inoculated in 96 porocytes and cultivates version, 100 μ l/ holes, 37 ℃, 5% CO
2Overnight incubation.
2) drug treating: every group of medicine be with the final concentration administration of 40 μ g/ml, 200 μ g/ml, 1000 μ g/ml, the every hole of 100 μ l/, every group three multiple hole.
3) suppressing active detects: drug treating is after 15 minutes, and the LPS irritation cell activation NF-κ B with final concentration 1 μ g/ml continues to cultivate 6 hours, and blank control group does not add LPS to do contrast with the volume culture medium.
4) activate active the detection: drug treating group and blank control group all do not add LPS and add with the volume culture medium, and the LPS irritation cell that matched group adds final concentration 1 μ g/ml activates NF-κ B, continues to cultivate 6 hours.
5) discard culture fluid, with 20 μ l/ hole 1 * CCLR dissolved cells.
6) add 40 μ l/ hole Luciferin, in 10 minutes, read the RLU value.
7) calculate:
3. result:
Each organizes the suppression ratio of sample to NF-κ B
Each organizes the activity ratio of sample to NF-κ B
The chemical structure characteristic of spirulina polysaccharide of the present invention is: main chain is by 1; 4-connects alpha-D-glucose and constitutes, and on average per 8 main chain residues a branch is arranged, and is substituted in 6 of branch point; Branch is made up of the glucoses of 1 or 216 connection, and structural formula is following:
And by the preparation of following method:
Get 5.0 kg spirulina raw medicinal herbs, add 20 L, 95% industrial alcohol, soaked seven days under the room temperature, with weeding of grease dissolubility micromolecule.The ethanol that inclines, solids places the ventilation to dry, and extracts with boiling water then, adds about 30 L of entry at every turn, extraction time is 4 hours, with the sugared content of phenolsulfuric acid method Detection and Extraction liquid, to the sugar reaction not obvious till, extract altogether 5 times.Each extracting solution merged after filtering, and heating is concentrated into small size, to circulating water dialysis 2 days; It is centrifugal that dialyzed solution concentrates the back; 1:1 (v/v) adds 30% trichloroacetic acid solution removal albumen, and reaction 4 hours under 4 degree conditions, adding 10%NAOH solution, to be neutralized to pH value be that bag is pricked to circulating water dialysis 48 hours in about 7 backs, and it is centrifugal that dialyzed solution concentrates the back; Supernatant adds 95% long-pending industrial alcohol of triploid, hold over night.Centrifugal collecting precipitation, respectively behind dehydrated alcohol and washing with acetone, in 80 ℃ of dryings, water to carry crude polysaccharides LXZ-W be 99 g.
Solid residue after the boiling water extraction with 5% (v/v) NaOH solution, 20 L lixiviates 3 hours, intermittently stirs under 4 ℃, filters; Be neutralized to pH about 7 with 10% (v/v) HCl solution, prick bag to circulating water dialysis 2 days, it is centrifugal that dialyzed solution concentrates the back; Supernatant adds the long-pending 95% industrial alcohol deposition of triploid, hold over night, centrifugal collecting precipitation; Behind dehydrated alcohol and washing with acetone,, get alkali and carry crude polysaccharides LXZ-N 6.4 g respectively in 80 ℃ of dryings.
Water is carried the spirulina crude polysaccharides and is separated with DEAE-cellulose anion-exchange column (50 cm * 5 cm) chromatography; With deionized water and different ionic strength (0.1; 0.2; 0.3 NaCl solution and 0.5 mol/L) carries out stepwise elution, utilizes the DEAE-cellulose that the absorption affinity different features of different structure polysaccharide is divided into different fraction polysaccharides with crude polysaccharides.Water is carried crude polysaccharides LXZ (10 g) and is gone up appearance (10 g samples are dissolved in 100 ml deionized waters, and appearance is gone up in centrifugal back), respectively from deionized water, 0.1,0.2; 0.3 obtain five components with separating in the eluent of 0.5 mol/L NaCl solution, eluent is dialysed to deionized water, dialyzed solution concentrating under reduced pressure and lyophilization; Products therefrom is called after LXZ-W respectively, LXZ-0.1, LXZ-0.2; LXZ-0.3, LXZ-0.5.
Get LXZ-W sample 100 mg, with the 0.2mol/L sodium chloride dissolving of 10ml, centrifugal, S-300 gel column on the supernatant gets the LXZ-W-300 component with 0.2 mol/L sodium chloride eluent eluting, and detecting through HPLC is a holosaccharide, and molecular weight is 7.4 * 10
4About Da.
Instrument and equipment:
SHB-III circulation ability of swimming is used vacuum pump more: Great Wall, Zhengzhou science, industry and trade is prone to company limited.
85-2 type constant temperature blender with magnetic force: Shanghai Si Le Instr Ltd..
DHG-9070A type electric heating constant temperature air dry oven: Shanghai one permanent Science and Technology Ltd..
N-1100D-WD rotary evaporation in vacuo appearance: Japanese EYELA company.
UV260 visible spectrophotometer: Japanese Shimadzu.
GL-21M low-temperature and high-speed centrifuge: Shanghai centrifuge institute company limited.
The large-scale freezer dryer of Labconco: U.S. Labconco company.
Edwards small frozen drying machine: Britain Edwards company.
LKB 2211 automatic collectors: Sweden LKB company.
Claims (3)
1. the application of a spirulina polysaccharide is characterized in that spirulina polysaccharide is used to prepare the preparation that suppresses tumor growth.
2. the application of a spirulina polysaccharide is characterized in that spirulina polysaccharide is used to prepare the preparation of pair cell Wheat Protein.
3. the application of a spirulina polysaccharide is characterized in that spirulina polysaccharide is used to prepare the NF-KB immune formulation.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101053574A (en) * | 2006-04-10 | 2007-10-17 | 北京天新园医药科技开发有限公司 | Medicinal composition containing chitoser ester and preparation method and application thereof |
| CN101575359A (en) * | 2008-05-05 | 2009-11-11 | 青岛海之骄生物科技有限公司 | Extraction method of water-soluble spirulina phycobiliprotein |
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2012
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101053574A (en) * | 2006-04-10 | 2007-10-17 | 北京天新园医药科技开发有限公司 | Medicinal composition containing chitoser ester and preparation method and application thereof |
| CN101575359A (en) * | 2008-05-05 | 2009-11-11 | 青岛海之骄生物科技有限公司 | Extraction method of water-soluble spirulina phycobiliprotein |
Non-Patent Citations (2)
| Title |
|---|
| 刘志伟，等: "螺旋藻多糖研究进展", 《中国食物与营养》 * |
| 孙鹏，等: "螺旋藻多糖的结构及生物学活性研究", 《中国生化药物杂志》 * |
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