CN102640857A - Preparation method of rice active peptide chelated copper - Google Patents
Preparation method of rice active peptide chelated copper Download PDFInfo
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- CN102640857A CN102640857A CN2012101478401A CN201210147840A CN102640857A CN 102640857 A CN102640857 A CN 102640857A CN 2012101478401 A CN2012101478401 A CN 2012101478401A CN 201210147840 A CN201210147840 A CN 201210147840A CN 102640857 A CN102640857 A CN 102640857A
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- rice
- active peptide
- chelated copper
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- peptide chelated
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Abstract
The invention discloses a preparation method of rice active peptide chelated copper. The preparation method comprises the following steps of adding rice protein powder after being smashed into powder of 80 meshes in water; then adding trypsin, wherein the mass ratio of trypsin to the rice protein powder to water is 1: 50: (200-300), and the trypsin activity is 118mu/mg; then regulating the pH value to 7.5 with NaOH; and hydrolyzing for 6 hours under the condition of 45 DEG C, thereby obtaining hydrolysate of rice active peptide; then adding deionized water with the volume of 3-5 times of hydrolysate and CuSO4.H2O with the mass of 15-40% of hydrolysate; and at room temperature, stirring and reacting for 3-4 hours, adding absolute ethyl alcohol until sediment is not generated any more, washing the sediment with the deionized water and drying after centrifugal separation by a centrifugal machine, so as to obtain the rice active peptide chelated copper. The rice active peptide chelated copper prepared by the method provided by the invention has high purity and production total yield, also has a simple and convenient technology, is low in cost, and is suitable for scale production.
Description
Technical field
The invention belongs to field of feed additive technology, relate in particular to a kind of preparation method who in the animal technical field, cooks the rice active peptide chelated copper of nutriment.
Background technology
Peptide is the compounds of molecular structure between amino acid and protein, is the 26S Proteasome Structure and Function fragment of protein, and is that protein has the physiological function of counting in necessarily.The rice active peptide is meant the peptides that the vital movement to living organism is useful or have physiological function, claims Functional Polypeptides again.The rice active peptide is the same with amino acid also to be low-molecular-weight bioligand important in the organism; But different with amino acid, the rice active peptide not only participates in these micro-complexings and metabolism directly and itself just has crucial function in organism.
Chelate is meant one or more groups and the formed compound with circulus of metal ion generation compatibility reaction.A metallic element ion can form the ring chelate with a plurality of amino acid, and the number of rings of formation is many more, and the stability of chelate is good more.This organic molecule that obtains through strong coordinate bond chelating (amino acid or rice active peptide) and the compound of metal ion are widely used in the animal technical field; Its validity is from the biologically active of related metal ion, and metal ion plays a role as conditioning agent as activator and in the various metabolism of all biologic artifacts in the reaction of many enzymes.
Metal ion since with the chelating of the key of peptide chain or amino acid material, help absorption, utilization and the use of metal ion, because it is transported to organic All Ranges through organic molecule.Especially in the animal technical field, these compounds are used for the animal production field with increase or enhancing normal metabolic and functional activity, thereby the output property energy for growth of animal is had significant positive influences.
Summary of the invention
Content of the present invention provides a kind of preparation method of rice active peptide chelated copper, and technology of the present invention is easy, with low cost, is suitable for large-scale production.
The objective of the invention is to realize through following technical scheme: a kind of preparation method of rice active peptide chelated copper, this method is: added in the entry after rice protein is pulverized 80 orders, and added trypsase again; The mass ratio of trypsase, rice protein powder and water is 1:50:200-300, and tryptic activity is 118u/mg, regulates pH to about 7.5 with NaOH then, and about 6 h of hydrolysis under 45 ℃ of conditions must contain the hydrolyzate of rice active peptide; Adding volume then is 3-5 times deionized water of hydrolyzate and
of the 15-40% that quality is hydrolyzate; At room temperature, stirring reaction added till absolute ethyl alcohol to the no longer generation deposition after 3～4 hours, after centrifugalizing with centrifuge, spent deionised water deposition and dry, finally obtained rice active peptide chelated copper.
The invention has the beneficial effects as follows that the present invention utilizes copper sulphate and the little peptide characteristic that is insoluble to ethanol soluble in water, when purifying, adopt ethanol to separate out crystallization, the product purity that obtains, overall recovery height, product is loose uniformly crystal, Functionality, quality and appealing design.Technology of the present invention is easy, with low cost to be appropriate to the scale of chemical production.
The specific embodiment
The preparation method of rice active peptide chelated copper is specially: added in the entry after rice protein is pulverized 80 orders; Add trypsase again; The mass ratio of trypsase, rice protein powder and water is 1:50:200-300, and tryptic activity is 118u/mg, regulates pH to 7.5 with NaOH then; Hydrolysis 6 h under 45 ℃ of conditions must contain the hydrolyzate of rice active peptide.This moment, the degree of hydrolysis of hydrolyzate was about 30%, and yield is more than 35%; The trichloroacetic acid soluble nitrogen is 94.2%; The average peptide chain length of estimation is about 3.3, shows that this enzymolysis product mainly is a small-molecular peptides.Adding volume then is 3-5 times deionized water of hydrolyzate and
of the 15-40% that quality is hydrolyzate.At room temperature, stirring reaction adds till absolute ethyl alcohol to the no longer generation deposition after 3～4 hours at last; After rice active peptide chelated copper is precipitated out; After centrifugalizing with centrifuge, spend deionised water and dry, finally obtain rice active peptide chelated copper.
The present invention combines following instance to further specify, and it is more obvious that the object of the invention will become.
Embodiment 1
Select the active trypsase of 118u/mg that is for use; 500 grams were pulverized 80 purpose rice proteins be dissolved in 2 liters of distilled water and process suspension, added 10 gram trypsase then, regulated pH to 7.5. hydrolysis 4 hours under 45 ℃ of conditions with NaOH; Then enzymolysis liquid is removed by filter impurity, subsequent use.At room temperature in reactor, add distilled water; The amount that adds is 3 times of subsequent use enzymolysis liquid; Press 15% adding
of the quality of subsequent use enzymolysis liquid, agitator makes its dissolving, and stirring reaction is after 3 hours; Adding absolute ethyl alcohol is precipitated out chelate; Centrifugalize with centrifuge then, it is dry then to spend deionised water after the separation, finally obtains rice active peptide chelated copper.
Embodiment 2
Select the active trypsase of 118u/mg that is for use; 500 grams were pulverized 80 purpose rice proteins be dissolved in 3 liters of distilled water and process suspension, added 10 gram trypsase then, regulated pH to 7.5. hydrolysis 4 hours under 45 ℃ of conditions with NaOH; Then enzymolysis liquid is removed by filter impurity, subsequent use.At room temperature in reactor, add distilled water; The amount that adds is 5 times of subsequent use enzymolysis liquid; Press 40% adding
of the quality of subsequent use enzymolysis liquid, agitator makes its dissolving, and stirring reaction is after 4 hours; Adding absolute ethyl alcohol is precipitated out chelate; Centrifugalize with centrifuge then, it is dry then to spend deionised water after the separation, finally obtains rice active peptide chelated copper.
The foregoing description is used for the present invention that explains, rather than limits the invention, and in the protection domain of spirit of the present invention and claim, any modification and change to the present invention makes all fall into protection scope of the present invention.
Claims (1)
1. the preparation method of a rice active peptide chelated copper is characterized in that, this method is: added in the entry after rice protein is pulverized 80 orders, and added trypsase again; The mass ratio of trypsase, rice protein powder and water is 1:50:200-300, and tryptic activity is 118u/mg, regulates pH to about 7.5 with NaOH then, and about 6 h of hydrolysis under 45 ℃ of conditions must contain the hydrolyzate of rice active peptide; Adding volume then is 3-5 times deionized water of hydrolyzate and
of the 15-40% that quality is hydrolyzate; At room temperature, stirring reaction added till absolute ethyl alcohol to the no longer generation deposition after 3～4 hours, after centrifugalizing with centrifuge, spent deionised water deposition and dry, finally obtained rice active peptide chelated copper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101478401A CN102640857A (en) | 2012-05-14 | 2012-05-14 | Preparation method of rice active peptide chelated copper |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101478401A CN102640857A (en) | 2012-05-14 | 2012-05-14 | Preparation method of rice active peptide chelated copper |
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| CN102640857A true CN102640857A (en) | 2012-08-22 |
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| CN2012101478401A Pending CN102640857A (en) | 2012-05-14 | 2012-05-14 | Preparation method of rice active peptide chelated copper |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105661049A (en) * | 2016-03-17 | 2016-06-15 | 上海源耀生物股份有限公司 | Polypeptide copper chelate prepared by using soybean protein isolate as substrate and preparation method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101756041A (en) * | 2010-01-19 | 2010-06-30 | 浙江大学 | Method for preparing casein peptide chelated copper |
| CN102286105A (en) * | 2011-06-14 | 2011-12-21 | 中国农业大学 | Sesame protein source metal chelating peptide and peptide trace element chelate and application thereof |
-
2012
- 2012-05-14 CN CN2012101478401A patent/CN102640857A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101756041A (en) * | 2010-01-19 | 2010-06-30 | 浙江大学 | Method for preparing casein peptide chelated copper |
| CN102286105A (en) * | 2011-06-14 | 2011-12-21 | 中国农业大学 | Sesame protein source metal chelating peptide and peptide trace element chelate and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 张全才等: "米蛋白肽锌的制备工艺研究", 《食品科技》, no. 2, 31 December 2008 (2008-12-31), pages 110 - 113 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105661049A (en) * | 2016-03-17 | 2016-06-15 | 上海源耀生物股份有限公司 | Polypeptide copper chelate prepared by using soybean protein isolate as substrate and preparation method |
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Application publication date: 20120822 |