CN102636645A - sTERM-1 enzyme-linked immunodetection kit and method thereof - Google Patents
sTERM-1 enzyme-linked immunodetection kit and method thereof Download PDFInfo
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- CN102636645A CN102636645A CN2012101216589A CN201210121658A CN102636645A CN 102636645 A CN102636645 A CN 102636645A CN 2012101216589 A CN2012101216589 A CN 2012101216589A CN 201210121658 A CN201210121658 A CN 201210121658A CN 102636645 A CN102636645 A CN 102636645A
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Abstract
The invention discloses a sTERM-1 enzyme-linked immunodetection kit which comprises (1) an ELISA (enzyme-linked immunosorbent assay) plate and a detection antibody and (2) a reactant and a detection agent, wherein a capturing antibody capable of generating an antigen-antibody reaction with sTERM-1 covers and is fixed on the ELISA plate; the detection antibody can generate an antigen-antibody reaction with sTERM-1 or a combination obtained after the antigen-antibody reaction between the sTERM-1 and the capturing antibody; and the reactant and detection agent are used for detecting whether the sTERM-1 exists in a sample to be detected through enzyme-linked immunodetection. The scheme of the invention is simple in step and short in reaction time, reduces the difference of repeated experiments, is suitable for clinical detection and has great guiding significance to the prevention and diagnosis of multiple diseases, particularly acute pneumonia.
Description
Technical field
The present invention relates to field of biomedicine technology, relate in particular to the preparation method of a kind of sTERM-1 enzyme-linked immunologic detecting kit, this kit and adopt this kit to detect the method for sTERM-1.
Technical background
TREM is the immunoglobulin superfamily activated receptor that is expressed in myeloid cell of recent findings; Present confirmed 5 members that have at least; Wherein TREM-1 optionally is expressed in neutrophil leucocyte and monocyte, and the signal transduction pathway of mediation plays an important role in the generation of inflammatory reaction and cascade are amplified.
Although the TREM-1 native ligand is also not clear and definite at present; But discover that using the competitive monoclonal antibody of TREM-1 to activate TREM-1 can cause that PMN takes off particle; Cause short inflammatory factor and proteolytic enzyme to discharge; Respiratory burst and cytophagy; And this effect can produce synergy with the part of Toll-like acceptor (TLR) such as LPS etc., and the mortality damage that utilization soluble T REM-1 fusion blocking-up TREM-1 causes can protect mouse to avoid LPS or bacterium septicopyemia the time.Research thinks that TREM-1 plays a key effect in the acute inflammatory reaction process, and regulating TREM-1 is the potential target spot of treatment diseases associated with inflammation.
TREM-1 soluble form sTREM-1 is a kind of secretion hypotype that lacks membrane spaning domain of the mRNA montage mutant code of TREM-1; STREM-1 can be released into body fluid and closely related with the infection order of severity when discovering the pulmonary infection process, can be used for estimating curative effect and evaluate its prognosis.As far as accepting patients with mechanical ventilation, the diagnosis of bacterial pneumonia and treatment are also very difficult.Bronchoalveolar lavage; The microorganism quantitative culture of the sample that obtains; The most specific instrument that is considered to mechanical ventilation correlativity pneumonia (VAP) diagnosis; Yet microbe growth can postpone to diagnose 24～48 h, will cause the use of unnecessary antibiotic like this, and all has fraud not have profit for the application of the empirical broad-spectrum antibiotic of the patient who does not have infectious diseases.Gibot etc. doubt to the patients with mechanical ventilation of pneumonia 148 examples and have carried out perspective study; Independently determine the diagnosis of pneumonia by two ICU experts that do not know patient sTREM-1 level; The result finds that the sTREM-1 level significantly raises in 38 routine community acquired pneumonia patients and the 46 routine VAP patient's BAL fluids (BALF); But 64 routine non-pneumonia patient sTREM-1 levels do not raise; STREM-1 raises and diagnoses the susceptibility of pneumonia is 98%, and specificity is 90%, and multivariable analysis shows; STREM-1 raises and can predict pneumonia better than clinical pulmonary infection scoring and cytokine levels; And type and other correlation of variables clinical and biologically of the inflammatory cell number among sTREM-1 level and chronic obstructive emphysema medical history, the BALF, bacterial infection are little, be a high degree of specificity, extremely sensitive independently prediction index, thereby the researcher think for the patient who accepts Failure Treated with Mechanical Ventilation; The fast detecting of sTREM-1 among the BALF will provide quick, strong evidence for the diagnosis and differential diagnosis of bacterium, fungal infection property pneumonia and non-pneumonia clinically.The sTREM-1 level confirms that also sTREM-1 is the potential mark of VAP in the non-direct BAL fluid of detection of dynamic VAP patients such as Determann.Nearest Horonenko etc. find that to sTREM-1 horizontal detection in VAP patient's exhaled breath condensate VAP can well diagnosed or differentiate to the sTREM-1 level in the exhaled breath condensate.Above result of study explanation TREM-1 is a kind of new acute pulmonary infection mark, and the early diagnosis, therapeutic evaluation and the prognosis that can be used for diseases associated with inflammation such as acute pulmonary infection are estimated.
Disclosed sTREM-1ELISA kit in the prior art mostly also all is not suitable for clinical diagnosis research, only the scientific experiment of suitable certain limit.All need carry out sample TREM-1 content prediction before the experiment, and sensing range is not high yet.Therefore, developing a kind of sTERM-1 enzyme-linked immunologic detecting kit, can detect the sTERM-1 that whether exists in the testing sample quickly and accurately, is those skilled in the art's problem demanding prompt solutions.
Summary of the invention
Deficiency to prior art; Technical matters to be solved by this invention is to provide a kind of sTERM-1 enzyme-linked immunologic detecting kit; Step is simple, and the reaction time is short, has reduced the otherness of repeated experiments; Be fit to the application of Clinical detection, the prevention and the diagnosis and treatment of multiple disease especially acute pneumonia are had great directive significance.
In order to solve the problems of the technologies described above; A kind of sTERM-1 enzyme-linked immunologic detecting kit provided by the invention; Comprise: (1) ELISA Plate and detection antibody; Encapsulate on the said ELISA Plate and be fixed with the capture antibody that antigen-antibody reaction can take place with sTERM-1, said detection antibody capable and sTERM-1 or with the combination generation antigen-antibody reaction of sTERM-1 after with capture antibody generation antigen-antibody reaction; (2) reactant and detection agent are used for having the material to deny through enzyme linked immunosorbent detection testing sample sTERM-1.
Preferably, said capture antibody is a monoclonal sTERM-1 antibody.More preferably, said capture antibody is a mouse-anti people sTERM-1 monoclonal antibody.
Preferably, said detection antibody is polyclone sTERM-1 antibody.More preferably, said detection antibody is the anti-people sTERM-1 of rabbit polyclonal antibody.
Preferably, said reactant comprises separately positive control solution, negative controls, 20 times of concentrated cleaning solutions of packing, sample buffer; Said detection agent comprises chromogenic substrate liquid A, substrate solution B and stop buffer.
Preferably, said detection antibody is marked with horseradish peroxidase through the HRP-coupling buffer, contains the solution of following material during said HRP-coupling buffer is every liter:
Tris 6.05g，
Nacl 8.4g，
Sucrose 20g,
BSA 10g，
CaCl2 100mg，
EDTA 0.374g，
Phenol 0.1g,
Thimerosal 0.01g.
Preferably, said capture antibody is fixed in the detection hole of said ELISA Plate through encapsulating damping fluid, saidly encapsulates the solution that damping fluid contains following material in being every liter:
NaCl： 43.25g，
Na2HPO4： 7.65g，
NaH2PO4： 1.3g；
After encapsulating processing, every hole adds 100-200 μ L bovine serum albumin solution washing 2-4 time, contains the solution of following material during said bovine serum albumin solution is every liter:
NaHPO
4.12?H
2O 3.72g，
KH
2PO
4 0.43g，
NaCl 6.?8g；
The confining liquid that in every hole, adds 200-300 μ L again seals processing, contains the solution of following material during said confining liquid is every liter:
BSA 10g，
Sucrose 20g,
Thimerosal 0.01g.
In addition, the present invention also provides a kind of preparation method of sTERM-1 enzyme-linked immunologic detecting kit, comprises the steps:
(1) every hole adds the capture antibody of 100ul5ug/ml/s on ELISA Plate, and 4 ℃ encapsulate reaction and spend the night, and the capture antibody of antigen-antibody reaction can take place with sTERM-1 said capture antibody;
(2) remove and encapsulate damping fluid, every hole adds 100-200 μ L bovine serum albumin solution washing 2-4 time, contains the solution of following material during said bovine serum albumin solution is every liter:
NaHPO
4.12?H
2O 3.72g，
KH
2PO
4 0.43g，
NaCl 6.?8g；
The confining liquid that in every hole, adds 200-300 μ L again carried out capping 1-3 hour, contained the solution of following material during said confining liquid is every liter:
BSA 10g，
Sucrose 20g,
Thimerosal 0.01g.
Preferably; The preparation method of described sTERM-1 enzyme-linked immunologic detecting kit; Also comprise through the HRP-coupling buffer and horseradish peroxidase is arranged in detection antibody marked; Detect antibody marked horseradish peroxidase, said detection antibody capable and sTERM-1 perhaps with the combination generation antigen-antibody reaction of sTERM-1 after with capture antibody generation antigen-antibody reaction, contain the solution of following material during said HRP-coupling buffer is every liter:
Tris 6.05g，
Nacl 8.4g，
Sucrose 20g,
BSA 10g，
CaCl2 100mg，
EDTA 0.374g，
Phenol 0.1g,
Thimerosal 0.01g.
Moreover the present invention also provides a kind of sTERM-1 enzyme-linked immune detection method, comprises the steps:
(1) is fixed with and takes place to add detected sample or sTREM-1 standard antigen 50-100ul/ hole on the ELISA Plate of capture antibody of antigen-antibody reaction with sTERM-1 encapsulating, 37 ℃ of reactions 0.5-1.5 hour;
(2) remove reaction liquid in the step 1, with lavation buffer solution washing 2-4 time; The sTREM-1 that adds 200ng/ml HRP mark detects antibody 50-200ul/ hole, 37 ℃ of reactions 20-40 minute, said detection antibody capable and sTERM-1 perhaps with the combination generation antigen-antibody reaction of sTERM-1 after with capture antibody generation antigen-antibody reaction;
(3) remove sTREM-1 and detect antibody-solutions, with lavation buffer solution washing 3-5 time; Every hole added 100ul chromogenic substrate room temperature reaction 10 minutes, and every then hole adds 1M HCL cessation reaction, and ELIASA 450nm wavelength readings is analyzed.
Preferably, said capture antibody is a monoclonal sTERM-1 antibody; More preferably, said capture antibody is a mouse-anti people sTERM-1 monoclonal antibody.Preferably, said detection antibody is polyclone sTERM-1 antibody; More preferably, said detection antibody is the anti-people sTERM-1 of rabbit polyclonal antibody.
Than prior art, the sTERM-1 enzyme-linked immunologic detecting kit, the dibit point one-step ELISA of utilizing the present invention to set up is simple owing to operation steps, and the reaction time is short, has reduced the otherness of repeated experiments.The capture antibody that is adopted in the kit of the present invention is the mouse-anti people sTERM-1 monoclonal antibody with high-affinity, and dibit point one-step ELISA susceptibility and specificity that the present invention is set up are stronger.Compare the application that double antibody sandwich method ELISA is more suitable for Clinical detection.Confirm through adopting method of the present invention and kit to detect in the 280 routine clinical samples of collecting; Technical scheme of the present invention have detection time short, efficient is high; Sensing range (differs from 200 times greatly between least concentration and the maximum concentration; Other Company products is less than 100 times), LDL is near the advantage of 0.2ng/ml (other company is all more than 0.3ng/ml).
Embodiment:
For making the present invention be more prone to understand,, further set forth the present invention below in conjunction with specific embodiment.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.
The preparation of embodiment 1:sTERM-1 enzyme-linked immunologic detecting kit
Carry out as follows:
(1) encapsulate: every hole adds the capture antibody of 100ul5ug/ml/s on ELISA Plate, and 4 ℃ encapsulate reaction and spend the night, and the capture antibody of antigen-antibody reaction can take place with sTERM-1 said capture antibody;
(2) sealing: remove and encapsulate damping fluid, every hole adds 100-200 μ L bovine serum albumin solution washing 3 times, contains the solution of following material during said bovine serum albumin solution is every liter:
NaHPO
4.12?H
2O 3.72g，
KH
2PO
4 0.43g，
NaCl 6.?8g；
The confining liquid that in every hole, adds 200-300 μ L again carried out capping 2 hours, contained the solution of following material during said confining liquid is every liter:
BSA 10g，
Sucrose 20g,
Thimerosal 0.01g.
(3) preserve: will seal good microwell plate and get rid of liquid in the hole, clap and do, and spend the night, and put into the plastic bag sealing of drying agent then, and be stored in 4 ℃ of environment in 25'C (room temperature) environment held.
In addition; Also need in detection antibody marked horseradish peroxidase be arranged through the HRP-coupling buffer; Detect antibody marked horseradish peroxidase; Said detection antibody capable and sTERM-1 or with the combination generation antigen-antibody reaction of sTERM-1 after with capture antibody generation antigen-antibody reaction, contain the solution of following material during said HRP-coupling buffer is every liter:
Tris 6.05g，
Nacl 8.4g，
Sucrose 20g,
BSA 10g，
CaCl2 100mg，
EDTA 0.374g，
Phenol 0.1g,
Thimerosal 0.01g.
In a preferred embodiment of the invention; STERM-1 enzyme-linked immunologic detecting kit according to the such scheme preparation has good detection effect; Certainly in other embodiments of the invention, suitably adjustment encapsulates time, wash time and the off-period of processing, the concentration of used each solution; Can guarantee to solve under the prerequisite of technical matters of the present invention, also be to be understood that to belonging to the scope that claim of the present invention contains.
Embodiment 2: sample detection
(1) is fixed with and takes place to add detected sample or sTREM-1 standard antigen 100ul/ hole on the ELISA Plate of capture antibody of antigen-antibody reaction with sTERM-1 encapsulating, 37 ℃ of reactions 1 hour;
(2) remove reaction liquid in the step 1, with lavation buffer solution washing 3 times; The sTREM-1 that adds 200ng/ml HRP mark detects antibody 50-200ul/ hole, 37 ℃ of reactions 30 minutes, said detection antibody capable and sTERM-1 perhaps with the combination generation antigen-antibody reaction of sTERM-1 after with capture antibody generation antigen-antibody reaction;
(3) remove sTREM-1 and detect antibody-solutions; With lavation buffer solution washing 5 times; Every hole added 100ul chromogenic substrate room temperature reaction 10 minutes, and every then hole adds 1M HCL cessation reaction, and ELIASA 450nm wavelength readings is analyzed.
In a preferred embodiment of the invention; STERM-1 in the test sample has good effect according to the method described above; Certainly in other embodiments of the invention, suitably adjustment encapsulates time, wash time and the intermission of reaction, the concentration of used each solution; Can guarantee to solve under the prerequisite of technical matters of the present invention, also be to be understood that to belonging to the scope that claim of the present invention contains.
Need to prove, in embodiments of the present invention, specimen in use collect from Subsidiary Hospital of Guangdong Medical College, Guangzhou chest hospital and, the middle mountain First Academy, amount to 280 routine clinical samples, be and be diagnosed as the sample that acute pneumonia infects clinically; Normal healthy controls group 30 examples from Subsidiary Hospital of Guangdong Medical College's MEC, do not have clinical symptoms in addition, and are no abnormal through health check-up.All samples all adopt the blood sampling of elbow medium sized vein, and 37 ℃ leave standstill 2h, behind the separation of serum-20 ℃ of preservations subsequent use, avoid multigelation.
In addition, for the important reagent and the sample that are adopted in the embodiment of the invention, its source or collocation method are following:
Capture antibody is a mouse-anti people sTERM-1 monoclonal antibody, buys from invitrogen company, and product batch number 201112035-7, detecting antibody is the anti-people sTERM-1 of rabbit polyclonal antibody, product batch number MQ20113025.Certainly in other embodiments of the invention, can solve under the prerequisite of technical matters of the present invention again, adopt the monoclonal sTERM-1 antibody of other kinds and polyclone sTERM-1 antibody to be to be understood that to belonging to the protection domain of claim of the present invention.
The solution that contains following composition during lavation buffer solution is every liter:
KH2PO4 0.2g
Na2HPO4.12H2O 2.9g
Nacl 8.0g
KCL 0.2g
Tween-20 0.5ml
With the dissolving of deionization sterilized water, pH value 7.4,2-8 ℃ of preservation, dress is the concentrate of 20 * lavation buffer solution in kit, direct dilution all can during use.
In addition, detection agent comprises chromogenic substrate liquid A, substrate solution B and stop buffer, wherein
The solution that contains following composition during chromogenic substrate liquid A is every liter:
Na2HPO4（12H2O） 36.82g
Citric acid 10.21g
Urea peroxide 0.6g
With the dissolving of deionization sterilized water, pH value 7.4,2-8 ℃ of preservation.
The solution that contains following composition during chromogenic substrate liquid B is every liter:
EDTA 0.146g
Citric acid 1.05g
TMB 0.25g
DMSO 5ml
In use, chromogenic substrate liquid A and chromogenic substrate liquid B by volume 1:1 are mixed into working fluid, join existing usefulness at present.
Through adopting the 280 routine clinical positive sample that method of the present invention and kit detect to collect and the detection test of 50 routine negative samples to draw, disclosed method and kit lowest detection are limited to 0.2ng/ml in the above-mentioned enforcement, and sensitivity is high; And positive sample detect in detect 269 altogether, positive sample detects and certainly goes rate up to 96%, does not contain sTERM-1 and all detect in the 50 routine negative samples, therefore kit of the present invention has extraordinary accuracy; In addition, this law adopts dibit point one-step ELISA to carry out test experience, and operation steps is simple, and the reaction time is short, and can reduce the otherness of repeated experiments.
Should be noted that; Above embodiment is only in order to technical scheme of the present invention to be described but not to the restriction of protection domain of the present invention; Although the present invention has been done detailed description with reference to preferred embodiment; Those of ordinary skill in the art should be appreciated that and can make amendment or be equal to replacement technical scheme of the present invention, and do not break away from the essence and the scope of technical scheme of the present invention.
Claims (10)
1. sTERM-1 enzyme-linked immunologic detecting kit; It is characterized in that; This kit comprises (1) ELISA Plate and detects antibody; Encapsulate on the said ELISA Plate and be fixed with the capture antibody that antigen-antibody reaction can take place with sTERM-1, said detection antibody capable and sTERM-1 or with the combination generation antigen-antibody reaction of sTERM-1 after with capture antibody generation antigen-antibody reaction; (2) reactant and detection agent are used for having the material to deny through enzyme linked immunosorbent detection testing sample sTERM-1.
2. sTERM-1 enzyme-linked immunologic detecting kit according to claim 1 is characterized in that, said capture antibody is a mouse-anti people sTERM-1 monoclonal antibody.
3. sTERM-1 enzyme-linked immunologic detecting kit according to claim 1 is characterized in that, said capture antibody is the anti-people sTERM-1 of a rabbit polyclonal antibody.
4. sTERM-1 enzyme-linked immunologic detecting kit according to claim 1 is characterized in that, said reactant comprises separately positive control solution, negative controls, 20 times of concentrated cleaning solutions of packing, sample buffer; Said detection agent comprises chromogenic substrate liquid A, substrate solution B and stop buffer.
5. sTERM-1 enzyme-linked immunologic detecting kit according to claim 1 is characterized in that, said detection antibody is marked with horseradish peroxidase through the HRP-coupling buffer, contains the solution of following material during said HRP-coupling buffer is every liter:
Tris 6.05g，
Nacl 8.4g，
Sucrose 20g,
BSA 10g，
CaCl2 100mg，
EDTA 0.374g，
Phenol 0.1g,
Thimerosal 0.01g.
6. sTERM-1 enzyme-linked immunologic detecting kit according to claim 1 is characterized in that, said capture antibody is fixed in the detection hole of said ELISA Plate through encapsulating damping fluid, saidly encapsulates the solution that damping fluid contains following material in being every liter:
NaCl： 43.25g，
Na2HPO4： 7.65g，
NaH2PO4： 1.3g；
After encapsulating processing, every hole adds 100-200 μ L bovine serum albumin solution washing 2-4 time, contains the solution of following material during said bovine serum albumin solution is every liter:
NaHPO
4.12?H
2O 3.72g，
KH
2PO
4 0.43g，
NaCl 6.?8g；
The confining liquid that in every hole, adds 200-300 μ L again seals processing, contains the solution of following material during said confining liquid is every liter:
BSA 10g，
Sucrose 20g,
Thimerosal 0.01g.
7. the preparation method of a sTERM-1 enzyme-linked immunologic detecting kit is characterized in that, comprises the steps:
(1) every hole adds the capture antibody of 100ul5ug/ml/s on ELISA Plate, and 4 ℃ encapsulate reaction and spend the night, and the capture antibody of antigen-antibody reaction can take place with sTERM-1 said capture antibody;
(2) remove and encapsulate damping fluid, every hole adds 100-200 μ L bovine serum albumin solution washing 2-4 time, contains the solution of following material during said bovine serum albumin solution is every liter:
NaHPO
4.12?H
2O 3.72g，
KH
2PO
4 0.43g，
NaCl 6.?8g；
The confining liquid that in every hole, adds 200-300 μ L again carried out capping 1-3 hour, contained the solution of following material during said confining liquid is every liter:
BSA 10g，
Sucrose 20g,
Thimerosal 0.01g.
8. the preparation method of sTERM-1 enzyme-linked immunologic detecting kit according to claim 7; It is characterized in that: also comprise through the HRP-coupling buffer horseradish peroxidase being arranged in detection antibody marked; Detect antibody marked horseradish peroxidase; Said detection antibody capable and sTERM-1 or with the combination generation antigen-antibody reaction of sTERM-1 after with capture antibody generation antigen-antibody reaction, contain the solution of following material during said HRP-coupling buffer is every liter:
Tris 6.05g，
Nacl 8.4g，
Sucrose 20g,
BSA 10g，
CaCl2 100mg，
EDTA 0.374g，
Phenol 0.1g,
Thimerosal 0.01g.
9. a sTERM-1 enzyme-linked immune detection method is characterized in that, comprises the steps:
(1) is fixed with and takes place to add detected sample or sTREM-1 standard antigen 50-100ul/ hole on the ELISA Plate of capture antibody of antigen-antibody reaction with sTERM-1 encapsulating, 37 ℃ of reactions 0.5-1.5 hour;
(2) remove reaction liquid in the step 1, with lavation buffer solution washing 2-4 time; The sTREM-1 that adds 200ng/ml HRP mark detects antibody 50-200ul/ hole, 37 ℃ of reactions 20-40 minute, said detection antibody capable and sTERM-1 perhaps with the combination generation antigen-antibody reaction of sTERM-1 after with capture antibody generation antigen-antibody reaction;
(3) remove sTREM-1 and detect antibody-solutions, with lavation buffer solution washing 3-5 time; Every hole added 100ul chromogenic substrate room temperature reaction 10 minutes, and every then hole adds 1M HCL cessation reaction, and ELIASA 450nm wavelength readings is analyzed.
10. sTERM-1 enzyme-linked immune detection method according to claim 7 is characterized in that: said capture antibody is a mouse-anti people sTERM-1 monoclonal antibody; Said capture antibody is the anti-people sTERM-1 of a rabbit polyclonal antibody.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107561287A (en) * | 2017-08-30 | 2018-01-09 | 潍坊市康华生物技术有限公司 | A kind of vascular endothelial growth factor detection kit and its preparation and application |
| CN107576803A (en) * | 2017-08-30 | 2018-01-12 | 潍坊市康华生物技术有限公司 | A kind of S100 protein detection kits and its preparation and application |
| CN108680740A (en) * | 2018-05-21 | 2018-10-19 | 苏州佑君环境科技有限公司 | A kind of dilution horseradish peroxidase mark antigen solution and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070281319A1 (en) * | 2004-01-27 | 2007-12-06 | Bioxell S.P.A. | Method Of Diagnosing Infectious Disease By Measuring The Level Of Soluble Trem-1 In A Sample |
| CN102257387A (en) * | 2008-10-28 | 2011-11-23 | 巴黎公众助理医院 | Methods and kits for the rapid determination of patients at high risk of death during severe sepsis and septic shock |
| WO2012016333A1 (en) * | 2010-08-06 | 2012-02-09 | University Health Network | Biomarkers for malaria |
-
2012
- 2012-04-24 CN CN2012101216589A patent/CN102636645A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070281319A1 (en) * | 2004-01-27 | 2007-12-06 | Bioxell S.P.A. | Method Of Diagnosing Infectious Disease By Measuring The Level Of Soluble Trem-1 In A Sample |
| CN102257387A (en) * | 2008-10-28 | 2011-11-23 | 巴黎公众助理医院 | Methods and kits for the rapid determination of patients at high risk of death during severe sepsis and septic shock |
| WO2012016333A1 (en) * | 2010-08-06 | 2012-02-09 | University Health Network | Biomarkers for malaria |
Non-Patent Citations (2)
| Title |
|---|
| MARKUS P.RADSAK等人: "Soluble triggering receptor expressed on myeloid cells 1 is released in patients with stable chronic obstructive pulmonary disease.", 《CLINICAL AND DEVELOPMENTAL IMMUNOLOGY》 * |
| 杨朴强: "可溶性髓系细胞触发受体-1对脓毒症诊断价值及预后评价的研究", 《中国优秀硕士学位论文全文库》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107561287A (en) * | 2017-08-30 | 2018-01-09 | 潍坊市康华生物技术有限公司 | A kind of vascular endothelial growth factor detection kit and its preparation and application |
| CN107576803A (en) * | 2017-08-30 | 2018-01-12 | 潍坊市康华生物技术有限公司 | A kind of S100 protein detection kits and its preparation and application |
| CN108680740A (en) * | 2018-05-21 | 2018-10-19 | 苏州佑君环境科技有限公司 | A kind of dilution horseradish peroxidase mark antigen solution and preparation method thereof |
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