CN102628759A - Preparation and detection method of textile fiber digitized slice and microscopic examination simulation method - Google Patents
Preparation and detection method of textile fiber digitized slice and microscopic examination simulation method Download PDFInfo
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Abstract
The invention discloses a preparation and a detection method of textile fiber digitized slice and a microscopic examination simulation method. The digitized slice contains image information of a fiber sample loaded by a glass slice. The preparation method of the textile fiber digitized slice includes steps of: an image acquisition step: observing multiple sampling areas of the glass slice loaded with fiber sample by using a microscope; acquiring multiple direct observation images of multiple focus layers in each corresponding sampling areas, by using an image collecting device; and a digital slice generation step: storing the digitized slice into a computer readable storage medium, wherein the digitized slice includes all acquired direct observation images. Each image of the digitized slice prepared by the preparation method of the invention is a real digitized direct observation image, and contains complete original information, can be used to reproduce the detection process; therefore, it is especially suitable for teaching, training and detection, etc.
Description
Technical field
Present invention relates in general to the textile fibres detection technique, be specifically related to a kind of preparation method, detection method of textile fibres digitalized sliced sheet and with the method for computer simulation textile fibres sediments microscope inspection.
Background technology
Sediments microscope inspection is the main means that textile fibres detects, and the glass to liking a large amount of segment of fiber of carrying of its detection is cut into slices.Segment of fiber is cut out from fibrous bundle by various fiber segment devices, is blended in the paraffin wet goods liquid medium, and uniformly dispersing just becomes a glass section to be checked on microslide and covered again.
At present, conventional textile fibres detection means is that sediments microscope inspection is combined with computer-aided detection system, for example the serial fibre fineness analyser of existing CU etc. on the market.When detecting; Section to be checked is placed on the microscope stage; By the micro-image of image collecting device such as camera collection section to be checked and be transferred in the computing machine and show in real time by display; Through the image processing software in the computing machine image of being gathered is carried out analyzing and processing, with diameter and the cross-sectional sizes of measuring fiber and analyze kind under it.This detection mode is actually the detection of a kind of " online ".
There is following problem in this detection mode:
1. higher ageing requirement: require to have detected in the short period of time section sample (in general two or three hours).Because sample is present in the medium, the time has been grown its physicochemical property and can change, the accuracy that influence detects.Obtain image and this two sub-processes of analysis image and in fact whole testing process has comprised that circulation carries out, and analytic process will be much more slowly than acquisition process, but the latter is only based on image and nonessential based on sample itself in fact.
2. the objectivity that detects requires: the fiber microscopy is that color intensity profile and the morphological change characteristics through fiber surface is used as basis for estimation, and because the multi-level focusing of the mutual superposition needs of some segment of fiber just can be seen these characteristics.But just can only obtain testing result because testing process finishes the back, can't see those multi-level image information of relying and judging, be difficult to embody the objectivity in the testing process.
3. repeatability requires reliably: owing to there is not the original sample of high conformity, make testing result can not guarantee reliable repeatability.For example will send a plurality of testing agencies to detect, just need a plurality of section samples of preparation, but obviously there is individual difference in sample room, the testing result of each testing agency may be inconsistent.If will check the correctness of testing result, owing to exist timeliness problem needs to prepare the section sample again, same owing to the otherness between the sample, thus cause its testing result accurately to check.
Chinese patent CN 101487801 B provide a kind of " full-automatic acquisition method for micro-image of textile fibre longitudinal cutting stock "; Can automatically accomplish the IMAQ of all fibres; Obtain the panorama sketch of sample and the image of each fiber, have time saving and energy saving advantage.But this method is owing to existing the search deterministic process of segment of fiber, so might lose the focusing picture of some fibre section.And most cases is can be overlapping between the segment of fiber in the practice, and fiber tends to cross over the burnt aspect of number strata, and it is not accurate enough to show this root fiber with the image of an aspect, because the information that provides is limited, can influences and judge and measure.
In recent years, some reports about digitalized sliced sheet were arranged.One Chinese patent application 201010531981.4 discloses " a kind of method of making digital slices ".This method is cut into slices the best image of variant regional inner focusing effect as benchmark image with glass; And utilize the sublayer image that the differing heights place obtains in the current region that the not good regional area of the focusing effect of benchmark image in the current region is replaced; Obtain fused images; Afterwards a plurality of fused images are spliced into integral body, realize the digitizing of glass section.But this method is not only more loaded down with trivial details in processing procedure; And final two-dimensional panoramic image is to merge through the picture to differing heights to obtain; It or not the direct observed image of " original "; This will inevitably lose some real observation information, if directly apply to detection, then can lose objectivity.
In fact, owing to be approximate cylindrical on most natural fiber microcosmic, be three-dimensional in essence, the information of depth direction is indispensable when kind is differentiated.So in the fiber of reality detects; The focusing process is not only in order to obtain distinct image; Also having an important purpose is exactly in the process of focal variation; Detection person's knowledge by rule of thumb intuitively tells the multiple information such as circling, thickness, rake angle degree of fiber surface scale structure, and this accurate detection for fiber sample is crucial.
It is contemplated that, when substituting real glass section with the digitalized sliced sheet that is loaded with the fiber sample image information, then can realize a kind of " off-line " detection mode (that is, be no longer dependent on microscope and on glass section in kind).
In the testing process of the textile fibres of reality; Detection for the various parameters of seized fiber; Especially for the judgement of kinds of fibers, particularly judging under the occasion (the for example differentiation of wool and cashmere) at some complicated kinds of fibers, is an empirical very strong job.For same section, different detection personnel (testing staff of for example testing staff of different testing agencies, or initial survey and reinspection), or even the same testing staff repeated detection of carrying out also can not adopt identical testing process.For example; The different detection process may be selected the observed image of different focusing aspects/height; Also possibly select on different fibers or the same fiber different at different position and different to come mark and measure fibre diameter and/or cross-sectional sizes, the plurality of fibers and/or the same fiber that also may make up zones of different come the comprehensive evaluation fibre morphology at the image of one or more different focusing aspects.In other words, in the different detection process, arbitrary zone of cutting into slices all might use at the image of arbitrary focus layer face.In addition, the teaching for fiber detects possibly simulate various possible testing processes, distinguishes suitable or unsuitable testing process with the instruction student.The multifaceted image information of this multizone that also need cut into slices.Prior art and above-mentioned patent/patented claim are then all powerless to these demands.
Summary of the invention
The object of the present invention is to provide a kind of fiber to detect the preparation method and the device of digitalized sliced sheet, the digitalized sliced sheet of its preparation not only can be used for detecting, and can be used to reproduce testing process, also can be used for the analog detection process.Another object of the present invention is to provide a kind of detection method of textile fibres digitalized sliced sheet, another purpose of the present invention is to provide a kind of method and device with computer simulation textile fibres sediments microscope inspection.
According to an aspect of the present invention, a kind of preparation method of textile fibres digitalized sliced sheet is provided, said digitalized sliced sheet contains the image information of the fiber sample that glass section loaded, and said preparation method comprises:
Image acquisition step: a plurality of sample area with microscope is cut into slices to the glass that is mounted with fiber sample are observed; In each sample area, obtain a plurality of direct observed image to a plurality of focusing aspects that should sample area with image collecting device;
Digitalized sliced sheet generates step: digitalized sliced sheet is stored in the computer-readable recording medium, and said digitalized sliced sheet comprises all direct observed images that obtained.
Preferably, said digitalized sliced sheet can also comprise the identification information of each direct observed image.Said identification information can comprise station location marker information, and said station location marker information comprises the positional information of the sample area under this direct observed image and the positional information of residing focusing aspect.
Preferably, said a plurality of sample area can evenly distribute in said glass section.Preferably, said a plurality of sample area can cover the whole or most area of said glass section.Preferably, said a plurality of sample area can have identical size, and its size can be confirmed according to the said microscopical visual field.Neighbouring sample zone in said a plurality of sample area can be local overlapping in the horizontal of said glass section and/or vertically, also can be not overlapping.
Preferably, said microscope can have the three-D electric objective table, is moved by the controller automatic control and adjustment; Said glass section is arranged on the said three-D electric objective table.
According to a further aspect in the invention, a kind of preparation facilities of textile fibres digitalized sliced sheet is provided, said digitalized sliced sheet contains the image information of the fiber sample that glass section loaded, and said preparation facilities comprises:
Microscope has the three-D electric objective table that is used to carry said glass section;
Be used to obtain the image collecting device of direct observed image of a plurality of focusing aspects of a plurality of sample area of said glass section, said image collecting device is arranged on said microscopical real image face place;
Be used to store the computer-readable recording medium of the said digitalized sliced sheet that comprises said direct observed image;
Be used for the said three-D electric objective table of regulating and controlling moved and controlled the Image Acquisition of said image collecting device in the horizontal direction with the stepping of vertical direction controller, said controller is connected with said three-D electric objective table, said image collecting device and said computer-readable recording medium.
Preferably, said image collecting device can be single anti-digital camera.Said image collecting device can also be industrial camera or digital camera head.
Preferably, said controller is made up of the computing machine that control program is housed.
According to a further aspect of the invention; A kind of detection method of textile fibres digitalized sliced sheet is provided; Be used for detecting the digitalized sliced sheet that the preparation method obtained according to textile fibres digitalized sliced sheet provided by the invention with computer-aided detection system, said detection method comprises:
Section imports step, is used for said digitalized sliced sheet is imported computer-aided detection system;
Step display is used for selecting the arbitrary direct observed image of said digitalized sliced sheet to be presented at the display device of said computer-aided detection system;
Detect step, be used for detecting according to the direct observed image that is shown.
Preferably, the detection method of textile fibres digitalized sliced sheet of the present invention can also comprise the output step, is used to export the testing result information that detects step.Preferably, detection method of the present invention can also comprise the digitalized sliced sheet step of updating, is used for testing result information and said digitalized sliced sheet are merged, and generates the digitalized sliced sheet of having examined.
In step display, can select the current direct observed image that needs demonstration through the input equipment of computer-aided detection system.
When said digitalized sliced sheet comprises the station location marker information of said direct observed image; Can confirm the station location marker information of the direct observed image that current needs show according to the input of said input equipment, and the direct observed image that will have this station location marker information shows through said display device.Preferably; Said input equipment can comprise the first input subset and the second input subset; Confirm to be used in the station location marker information of the direct observed image that current needs show to identify the positional information of the sample area under this direct observed image according to the input of the said first input subset, confirm to be used to identify in the station location marker information of direct observed image of current needs demonstration the positional information of the residing focusing aspect of this direct observed image according to the input of the said second input subset.
Preferably, the said first input subset can comprise the four direction key of computer keyboard.The left and right directions key of said four direction key is used for confirming the affiliated lateral attitude of sample area on the plane of said glass section of direct observed image of current needs demonstration; The up and down arrow keys of said four direction key is used for confirming the affiliated lengthwise position of sample area on the plane of said glass section of direct observed image of current needs demonstration.Preferably, the said second input subset can comprise the roller of computer mouse, and the scroll forward and backward of said roller is used for the height of the residing focusing aspect of direct observed image of definite current needs demonstration.
According to a further aspect of the invention, a kind of method with computer simulation textile fibres sediments microscope inspection is provided, has comprised:
Preparation method according to textile fibres digitalized sliced sheet provided by the invention prepares digitalized sliced sheet;
Said digitalized sliced sheet is imported computing machine;
Simulate moving of microscope stage with the input information of the input equipment of computing machine, selecting the current direct observed image that shows of needing,
Selected direct observed image is presented on the display device of said computing machine;
Direct observed image to being shown detects.
Preferably, said digitalized sliced sheet can comprise the station location marker information of said direct observed image, and said station location marker information can comprise the positional information of the sample area under this direct observed image and the positional information of residing focusing aspect.Said input equipment can comprise the first input subset and the second input subset; Confirm to be used in the station location marker information of the direct observed image that current needs show to identify the positional information of the sample area under this direct observed image according to the input of the said first input subset, with simulation microscope stage mobile on the x-y plane; Confirm to be used to identify in the station location marker information of the direct observed image that current needs show the positional information of the residing focusing aspect of this direct observed image according to the input of the said second input subset, with simulation microscope stage mobile on the z axle.Preferably, the said first input subset can comprise the four direction key of computer keyboard, the input of said four direction key be respectively applied for the simulation microscope stage on the x-y plane along laterally left and right sides both direction and vertically moving of former and later two directions.Preferably, the said second input subset can comprise the roller of computer mouse, and the scroll forward and backward of said roller is used to simulate microscope stage moving up and down along the z axle.
In accordance with a further aspect of the present invention; A kind of device with computer simulation textile fibres sediments microscope inspection is provided; The digitalized sliced sheet that comprises computing machine and embodied on computer readable, said digitalized sliced sheet comprise the direct observed image of a plurality of focusing aspects of a plurality of sample area that glass is cut into slices; Said computing machine comprises:
The first input subset that move of simulation microscope stage on the x-y plane;
The second input subset that move of simulation microscope stage on the z direction of principal axis;
The display device that shows direct observed image, the direct observed image that is shown is confirmed by the input of the said first input subset and the second input subset;
The computer aided detection module that the direct observed image that said display device showed is detected.
Preferably, the said first input subset comprise the four direction key of computer keyboard, the input of said four direction key simulate respectively microscope stage on the x-y plane along laterally left and right sides both direction and vertically moving of former and later two directions.
Preferably, the said second defeated son is gone into the roller that equipment comprises computer mouse, scroll forward and backward simulation microscope stage the moving up and down along the z axle of said roller.
Adopting each width of cloth image of digitalized sliced sheet of the preparation method preparation of digitalized sliced sheet of the present invention all is the direct observed image of digitizing of true fiber, through any splicing or fusion and other any Flame Image Process that possibly change the concept of reality measurement information.And this digitalized sliced sheet all has the image of a plurality of focusing aspects for each sample area, and the raw information that is therefore comprised is comparatively complete, can be used for repetition great majority even testing process arbitrarily.Therefore be specially adapted to impart knowledge to students, train, the preservation of detection and fibre image data etc.
Description of drawings
Fig. 1 is the preparation facilities synoptic diagram of textile fibres digitalized sliced sheet of the present invention.
Fig. 2 (a)-(c) exemplarily shows the three kind coverage modes of sample area in the glass section.
Fig. 3 obtains the synoptic diagram of a plurality of direct observed images of a plurality of focusing aspects in single sample area for digitalized sliced sheet of the present invention.
Fig. 4 is for being incorporated into the synoptic diagram in the direct observed image with testing result information with the form of figure in the digitalized sliced sheet of the present invention.
Fig. 5 is the device synoptic diagram with computer simulation textile fibres sediments microscope inspection of the present invention.
Embodiment
Describe the present invention in detail below in conjunction with accompanying drawing.
The preparation facilities of textile fibres digitalized sliced sheet
As shown in Figure 1, the preparation facilities of textile fibres digitalized sliced sheet of the present invention can comprise microscope 101, image collecting device 104 and controller 103.The microscope 101 preferred three-D electric objective tables 102 that self have; Certainly, this three-D electric objective table also can be realized through the object lens below that independent electric platforms is placed simple microscope.Controller 103 is connected with three-D electric objective table 102, image collecting device 104 and computer-readable recording medium 105.Moved with the stepping of vertical direction in the horizontal direction by controller 103 automatic control and adjustment three-D electric objective tables 102, the distance that stepping is moved can be provided with according to actual needs.Image collecting device 104 is arranged on the real image face place of microscope 101, and it can be single anti-digital camera, also can be industrial camera or digital camera head.By 104 automatic focuses of controller 103 control image collecting devices, obtain image and obtaining image stored in the computer-readable recording medium 105.This controller 103 can be realized with the computing machine that control program is housed.Computer-readable recording medium can be that computing machine carries or external memory device, like storer, and hard disk, DVD CD etc.
The preparation method of textile fibres digitalized sliced sheet
According to the present invention, the preparation method of textile fibres digitalized sliced sheet can be specific as follows said.
At first, in the glass section, mark off predetermined a plurality of sample area.Because the size of glass section is greater than microscopical objective lens field of view, microscope can only obtain the image of glass section in certain zone in each operation, therefore, in the glass section, marks off a plurality of sample area by the scope in the micro objective visual field.In one embodiment, these sample area are of similar shape and size.A plurality of sample area of being divided in one embodiment, preferably evenly distribute in the glass section.In one embodiment, these sample area preferably can cover the entire area of glass section or representative most area.The area that should be appreciated that the section of related glass here should be meant the effective coverage of the cover glass of glass section.
Adjacent sample area can be local overlapping in the horizontal of glass section and/or vertically, also can be not overlapping.More approaching not overlap mode when in general, manually-operated in the past detects.But, because the influence of the unequal factor of distribution of light in field of microscope may cause the edge of image zone not have zone line clear, also maybe be owing to some fibre information is lost in operating personnel's error.Adopt the partly overlapping mode of sample area to obtain image and can avoid above-mentioned unfavorable well, by contrast, do not have and can lose fiber information, and fiber information everywhere such as all can be represented clearly at advantage.But because sample area is overlapped, so the quantity of sample area can increase, the time of obtaining digitalized sliced sheet can prolong.
Fig. 2 (a)-(c) exemplarily shows the three kind coverage modes of sample area in the glass section.Fig. 2 (a) is a kind of all standing mode, and sample area is all overlapped on horizontal vertical both direction in twos, thus whole cover glass break area.Its lap may be selected to be the 1/4-1/3 of single sample area.Fig. 2 (b) is a kind of half coverage mode, and sample area is connected in the horizontal in twos, vertically goes up and separates divergence, is created in the several strips of horizontal complete cover glass section.Although not shown, be readily appreciated that, for half coverage mode, also can be connected in twos in the vertical, transversely separate divergence, be created in the several strips of vertical complete cover glass section.Equally, its lap may be selected to be the 1/4-1/3 of single sample area.Fig. 2 (c) is a kind of not coverage mode, and sample area is all separated on horizontal vertical both direction does not in twos have coincidence, is equivalent to glass break area uniform sampling.
The concrete sample area of how dividing can require to confirm according to the use occasion and the sampling rate of digitalized sliced sheet.For example, a large amount of actual detected fields is fashionable when being applied to, and then can select not coverage mode, can prepare digitalized sliced sheet more efficiently like this.When being applied to teaching occasions, not high to the time requirement that the preparation digitalized sliced sheet is required, then can select all standing or half coverage mode.When the sampling rate that requires digitalized sliced sheet is high or low, then can corresponding increase or reduce the dense degree that sample area distributes.
For image collecting device, can adopt single anti-digital camera or industrial camera or digital camera head.The applicant discovers; For identical glass section; When adopting identical sample area coverage mode; Use industrial camera or required time of digital camera head images acquired to be longer than and use single needed time of anti-digital camera, typically use single more than 5 times of anti-digital camera required time, under some coverage mode or even more than 10 times.Certainly, under the situation that reduces sampling rate greatly, also can use industrial camera or digital camera head, reasonably to accomplish IMAQ work in the time range.Therefore, for the coverage mode shown in Fig. 2 (a) and Fig. 2 (b), image collecting device preferably adopts single anti-digital camera.For the coverage mode shown in Fig. 2 (c), image collecting device is same preferred to adopt single anti-digital camera, but also can use industrial camera or digital camera head under the lower situation of sampling rate.
After predetermined way is as required divided a lot of sample area, then can observe one by one with a plurality of sample area that microscope is cut into slices to the glass that is mounted with fiber sample; In each sample area, obtain a plurality of direct observed image to a plurality of focusing aspects that should sample area with image collecting device.In this process, glass section is arranged on the three-D electric objective table, controller can automatic control and adjustment three-D electric objective table (in other words) and vertical direction (z direction of principal axis in other words) go up mobile on x-y the plane in the horizontal direction.By predetermined set a sample area of glass section is got in the microscopical object lens visual field; And it is moved to the predetermined in other words z shaft position of predetermined altitude; Be stored in the computer-readable recording medium again by the direct observed image of a width of cloth that obtains current focusing aspect in the current region after the automatic focus of controller adjusting image collecting device, and with it.Then controller can according to predetermined step distance control automatically the three-D electric objective table vertically in other words the z direction of principal axis move to another predetermined height of current sample area; Obtain the direct observed image of current focusing aspect after controlling image collecting device automatic focus by controller again, and be stored in the computer-readable recording medium.As shown in Figure 3, in single sample area, obtain a plurality of direct observed image at the place of focusing aspect in other words, a plurality of differing heights place.How a plurality of focusing aspects specifically are set, similar with aforementioned division sample area, can require to confirm according to the use occasion and the sampling rate of digitalized sliced sheet.For example, in one embodiment, can in each sample area, gather the direct observed image of the focusing aspect of 5-9 equal spacings.After accomplishing the IMAQ of a sample area, then by controller in the horizontal direction by the position of predetermined step distance moving three dimension motorized subject table, make in the microscopical object lens of another sample area shift-in visual field of this glass section.To the coverage mode shown in Fig. 2 (c), can travel through all sample area according to " bow " font move mode at Fig. 2 (a) through controller moving three dimension motorized subject table.So according to all images information of all focusing aspects of all sample area that just can obtain the section of whole glass with quadrat method, and store in the computer-readable recording medium, thereby obtained the digitalized sliced sheet of this glass section.In other embodiment, also can control the three-D electric objective table and in a focusing aspect, move and travel through all sample area, and then move to another focusing aspect and travel through all sample area.
The identification information that can also comprise in a preferred embodiment, the direct observed image of each width of cloth in the digitalized sliced sheet.This identification information can comprise station location marker information, like the positional information (like the position of sample area on the plane of glass section) of the sample area under this direct observed image and the positional information (as focusing on the height of aspect) of residing focusing aspect.Can the part of this station location marker information as digitalized sliced sheet be stored in the computer-readable recording medium.In one embodiment; This station location marker information can be used the form of three-dimensional coordinate; For example identify the position of sample area with x coordinate and y coordinate; Position with z coordinates logo focusing aspect makes arbitrary focusing aspect of arbitrary sample area can identify its position with unique three-dimensional coordinate.In other embodiments, also can adopt station location marker information such as the numeral and/or the letter etc. of other form, as long as make all corresponding unique station location marker information of arbitrary focusing aspect of arbitrary sample area.In digitalized sliced sheet, can direct observed image and its station location marker associating information be got up with the mode of data structure.Like this, computing machine can read corresponding direct observed image through its station location marker information.
Certainly, can also comprise the identification information of other type in the digitalized sliced sheet, for example microscopical enlargement factor or convert the scale that on direct observed image, marks to can accurately draw the physical size of seized fiber.Can also see that hereinafter the identification information of digitalized sliced sheet can also comprise the testing result information that the back is obtained that detects.
According to the prepared digitalized sliced sheet of the present invention can be as the glass section of sample in time or environmental baseline and changing, can be used as the parallel laboratory test of permanent preservation or the original sample of repetition measurement.So the result who detects repeatedly has more comparability.Thereby each section fibre in the digitalized sliced sheet can both have the clear state that can survey owing to obtain the fibre image of a plurality of focusing aspects; And it is that the glass section that breaks away from microscope and raw sample exists, thus be specially adapted to impart knowledge to students, train, the preservation of detection and fibre image data etc.
And; The direct observed image of the image information that is comprised in the digitalized sliced sheet according to the present invention's preparation for obtaining through microscope; It has reflected the direct observation information of glass section truly, and without any splicing or fusion and other any Flame Image Process that possibly change the concept of reality measurement information.
The detection method of textile fibres digitalized sliced sheet
After having obtained digitalized sliced sheet, can detect it at any time.Need elder generation that the digitalized sliced sheet of required detection is imported computer-aided detection system during detection.Computer-aided detection system can be the computing machine that has the dedicated test program.The input information of the input equipment that can be through computer-aided detection system such as the input equipment of mouse, keyboard or other type switches/selects the current direct observed image that shows of needing.In one embodiment, can realize the horizontal and vertical conversion of sample area, roll with mouse roller and realize the conversion of different focusing aspects through the four direction key of keyboard.When digitalized sliced sheet comprised the station location marker information of direct observed image, computer-aided detection system was confirmed station location marker information according to the input of its input equipment, and the direct observed image that this station location marker information is corresponding shows on display.In one embodiment, station location marker information can be aforesaid three-dimensional coordinate (x, y, form z); The directionkeys that clicks upwards representes that y value increases 1, and x, z value are constant, after operating like this; What show on the display then is that coordinate is (x, y+1, direct observed image z).Correspondingly, downward directionkeys representes that promptly the y value subtracts 1, left/and on behalf of the x value, right directionkeys subtract/add 1 respectively.The z of scrolling realization forward value through mouse roller adds 1, and scrolling realization z value subtracts 1 backward.In another embodiment; Also can be through the direct observed image of all sample area that show a certain focusing aspect is set; And the direct observed image of the direct observed image of each sample area and all different aspects of this sample area is linked; Make and to get into this sample area through a certain direct observed image of click, thereby can change the direct observed image that shows differing heights through mouse roller.
When with needed direct observed image after showing on the display; The testing staff then can carry out testing according to mode well-known to those skilled in the art; For example, can on the direct observed image that is shown, measure and mark the kind title of seized fiber and the measurement data in diameter or cross section, the gauge point information of diameter measurement; And the enumeration data of every kind of fiber etc., to obtain testing result information.These testing result information can merge with existing digitalized sliced sheet, generate to have examined digitalized sliced sheet.In one embodiment; Can direct observed image (if having station location marker information also to comprise this station location marker information) be associated with its testing result information with the mode of data structure; Computing machine then can obtain the relevant detection object information reading when examining digitalized sliced sheet like this.In another embodiment, also can the form of testing result information with figure be incorporated in the direct observed image, as shown in Figure 4.In Fig. 4; The picture in left side is for the real directly observed image that in the process of preparation digitalized sliced sheet, obtains and in testing process, directly be added on the markup information on the image, and the form on right side has provided the numbering of each the seized fiber that has marked among the corresponding left figure and diameter, kind accordingly.Like this, complete detection archives just are provided examining in the digitalized sliced sheet, have not merely helped auditing result, simultaneously as far as effective transparency and the confidence level that has increased testing result of client; But testing agency is provided the sample of a large amount of practical operations for internal training and external communication comparison.
After digitalized sliced sheet detected, can also export testing result information.For example, can go out the analytical statement of papery through printer prints.
The aforesaid computer-aided detection system that is used to detect digitalized sliced sheet can be through simply transforming and realize existing systems (like CU series fibre fineness analyser).For example revise the Data Input Interface of active computer assisted detection system, receive image that image collecting device transmits from " online " and change " off-line " into and read the digitalized sliced sheet the memory device.And the actual detection process can be carried out according to the mode of existing detection system fully.
The prepared digitalized sliced sheet of the present invention has been started brand-new detecting pattern: obtain image process and (carry out automatically; Obtain end and promptly mean the generation of digitalized sliced sheet) with testing process (the artificial participation; Based on digitalized sliced sheet, break away from microscope work) separate fully.These two processes needn't combine in a people (testing staff) again and combine in a machine (checkout equipment).Gather and detect and to carry out in different time by different people; Testing process can be interrupted at any time and do not taken equipment (microscope); Parallel experiment and check also and can carry out at any time.Testing process need not be considered the ageing of sample more in a word.Simultaneously, therefore less demanding to the operating personnel that obtain image because obtaining with testing process of digitalized sliced sheet separated, and the process of obtaining digitalized sliced sheet can realize robotization basically, only need understand that operating instrument gets final product.So just can make the scientific and reasonable division of labor: give professional testing agency (personnel) the professional work of tool (differentiating) and do such as kinds of fibers according to the present situation of manpower and device resource; Giving peripheral window (layman) work of the low technical content that stylizes (such as the fibre section sample preparation, gathering picture) does.Reasonable distribution manpower and equipment resource can be raised the efficiency and benefit greatly like this.
Utilize digitalized sliced sheet can realize the detecting pattern of " testing agency+a plurality of service windows ": core testing agency has the detection person who is rich in professional standing and experience of minority, and they use professional Survey Software that digitalized sliced sheet is detected; Core testing agency can have a plurality of strange lands service window under its command simultaneously; These windows only need be purchased the sample automatic acquisition equipment; Receive behind client's the sample only responsible use equipment and obtain digitalized sliced sheet and its higher level's core testing agency that passes oneself back through internet or express delivery CD is got final product, so the staff here need not detect professional knowledge in principle.It is convenient and branch out at low cost that this helps core testing agency.
Utilize digitalized sliced sheet can also realize the detecting pattern of " collecting device+many covers detect software " in addition: much the unit of detection has several professional detection persons usually; Can obtain digitalized sliced sheet with a collecting device specially to this, again each digitalized sliced sheet is distributed to many people detection.Help improving the treatment capacity of the business of detection.
Method and apparatus with computer simulation textile fibres sediments microscope inspection
Adopt the prepared digitalized sliced sheet of preparation method of textile fibres digitalized sliced sheet of the present invention can also be applied to computer simulation textile fibres sediments microscope inspection.Computer-readable recording medium 105 as shown in Figure 5, that the device with computer simulation textile fibres sediments microscope inspection of the present invention can comprise computing machine 501 and contain digitalized sliced sheet.Computing machine 501 can comprise that first is input into equipment 5011, second and is input into equipment 5012, display device 5013 and computer aided detection module 5014.
By first be input into equipment 5011 input simulation microscope stage moving on (representing to be the x-y plane) in the horizontal direction with three-dimensional coordinate; Input simulation microscope stage moving on height (representing to be the z direction of principal axis with three-dimensional coordinate) by the second input subset 5012; Display device 5013 shows the direct observed image of from digitalized sliced sheet, being selected by the input of the first input subset 5011 and the second input subset 5012.
Generally; Here so-called " simulation " is actually through the input of input equipment (like the first input subset 5011 and the second input subset 5012) and confirms to select in other words a station location marker information, then the direct observed image that has this station location marker information in the digitalized sliced sheet is presented on the display device 5013 (the for example display of computing machine) of computing machine.Like this, when user's operation input apparatus, similarly be at mobile microscopical objective table.Particularly, can confirm to select in other words the positional information of the sample area under the direct observed image in the digitalized sliced sheet according to the input of the first input subset 5011, like the position of sample area on the plane of glass section; Can confirm to select in other words the positional information of the direct residing focusing aspect of observed image in the digitalized sliced sheet according to the input of the second input subset 5012, as focusing on the height of aspect.Like this, when the user operates the first input subset, similarly be on the x-y plane, " to move " microscope stage, thereby on display device, show the direct observed image in different sample zone; And operate second when being input into equipment as the user, similarly be on the z direction of principal axis, " to move " microscope stage, thereby on display device, show the direct observed image of different focusing aspects.Like this, just reached the effect of " simulation " sediments microscope inspection.
The first input subset can comprise the four direction key of computer keyboard, the input of four direction key be respectively applied for the simulation microscope stage on the x-y plane along laterally left and right sides both direction and vertically moving of former and later two directions.The second input subset can comprise the roller of computer mouse, and the scroll forward and backward of roller is used to simulate microscope stage moving up and down along the z axle.
When simulation textile fibres sediments microscope inspection, at first digitalized sliced sheet is imported the computing machine that has computer aided detection module 5,014 501 as shown in Figure 5.According to aforesaid mode, simulate moving of microscope stage then, in order to select the current direct observed image that needs demonstration with the input equipment of computing machine.Detect by 5014 pairs of direct observed images that display device showed of computer aided detection module then.Computer aided detection module 5014 can be basic identical with the detection module in the existing computer-aided detection system.
Utilize this analogue means and method; Can in teaching and training, reproduce whole textile fibres sediments microscope inspection process, for example how utilize microscope to find a fiber, how differentiate its affiliated kind; How to measure its flexibility, measure its diameter or the like where.Because it is that color intensity profile and morphological change characteristics with fiber surface is basis for estimation that the kinds of fibers of fiber microscopy detects; Can obtain the foundation of judging through the direct observed image of the different levels in one or more sample area at computer simulation reproduction fiber place.
When digitalized sliced sheet of the present invention is used for teaching or computer simulation textile fibres sediments microscope inspection; This digitalized sliced sheet preferably has higher sampling rate; For example sample area adopts the mode of all standing and/or improves the quantity of focusing aspect in each sample area, thereby satisfies the required direct observed image of any testing process as much as possible.
Claims (22)
1. the preparation method of a textile fibres digitalized sliced sheet, said digitalized sliced sheet contains the image information of the fiber sample that the glass section loaded, and said preparation method comprises:
Image acquisition step: a plurality of sample area with microscope is cut into slices to the glass that is mounted with fiber sample are observed; In each sample area, obtain a plurality of direct observed image to a plurality of focusing aspects that should sample area with image collecting device;
Digitalized sliced sheet generates step: digitalized sliced sheet is stored in the computer-readable recording medium, and said digitalized sliced sheet comprises all direct observed images that obtained.
2. preparation method according to claim 1 is characterized in that said digitalized sliced sheet also comprises the identification information of each direct observed image.
3. preparation method according to claim 2 is characterized in that said identification information comprises station location marker information, and said station location marker information comprises the positional information of the sample area under this direct observed image and the positional information of residing focusing aspect.
4. according to each described preparation method among the claim 1-3, it is characterized in that said a plurality of sample area evenly distribute in said glass section.
5. according to each preparation method among the claim 1-4, it is characterized in that said a plurality of sample area have covered the whole or most area of said glass section.
6. according to each described preparation method among the claim 1-5, it is characterized in that said a plurality of sample area have identical size, its size is confirmed according to the said microscopical visual field.
7. according to each described preparation method among the claim 1-6, it is characterized in that, the neighbouring sample zone in said a plurality of sample area the section of said glass laterally and/or local overlapping vertically.
8. according to each described preparation method among the claim 1-6, it is characterized in that the neighbouring sample zone is not overlapping arbitrarily in said a plurality of sample area.
9. according to each described preparation method among the claim 1-8, it is characterized in that said microscope has the three-D electric objective table, move by the controller automatic control and adjustment; Said glass section is arranged on the said three-D electric objective table.
10. the detection method of a textile fibres digitalized sliced sheet is used for detecting the digitalized sliced sheet that is obtained according to each described preparation method of claim 1-9 with computer-aided detection system, and said detection method comprises:
Section imports step, is used for said digitalized sliced sheet is imported computer-aided detection system;
Step display is used for selecting the arbitrary direct observed image of said digitalized sliced sheet to be presented at the display device of said computer-aided detection system;
Detect step, be used for detecting according to the direct observed image that is shown.
11. detection method according to claim 10 is characterized in that, also comprises:
The output step is used to export the testing result information that detects step.
12., it is characterized in that according to claim 10 or 11 described detection methods, also comprise the digitalized sliced sheet step of updating, be used for testing result information and said digitalized sliced sheet are merged, generate the digitalized sliced sheet of having examined.
13. according to each described detection method among the claim 10-12, it is characterized in that, in step display, select the current direct observed image that needs demonstration through the input equipment of computer-aided detection system.
14. detection method according to claim 13; It is characterized in that; When said digitalized sliced sheet comprises the station location marker information of said direct observed image; Confirm the station location marker information of the direct observed image that current needs show according to the input of said input equipment, and the direct observed image that will have this station location marker information shows through said display device.
15. according to claim 13 or 14 described detection methods; It is characterized in that; Said input equipment comprises the first input subset and the second input subset; Confirm to be used in the station location marker information of the direct observed image that current needs show to identify the positional information of the sample area under this direct observed image according to the input of the said first input subset, confirm to be used to identify in the station location marker information of direct observed image of current needs demonstration the positional information of the residing focusing aspect of this direct observed image according to the input of the said second input subset.
16. detection method according to claim 15 is characterized in that, the said first input subset comprises the four direction key of computer keyboard.
17. detection method according to claim 16 is characterized in that, the left and right directions key of said four direction key is used for confirming the affiliated lateral attitude of sample area on the plane of said glass section of direct observed image of current needs demonstration; The up and down arrow keys of said four direction key is used for confirming the affiliated lengthwise position of sample area on the plane of said glass section of direct observed image of current needs demonstration.
18. according to each described detection method among the claim 15-17; It is characterized in that; The said second input subset comprises the roller of computer mouse, and the scroll forward and backward of said roller is used for the height of the residing focusing aspect of direct observed image of definite current needs demonstration.
19. the method with computer simulation textile fibres sediments microscope inspection comprises:
Prepare digitalized sliced sheet according to each described preparation method among the claim 1-9;
Said digitalized sliced sheet is imported computing machine;
Simulate moving of microscope stage with the input information of the input equipment of computing machine, to select the current direct observed image that shows of needing;
Selected direct observed image is presented on the display device of said computing machine;
Direct observed image to being shown detects.
20. method according to claim 19; It is characterized in that; Said digitalized sliced sheet comprises the station location marker information of said direct observed image, and said station location marker information comprises the positional information of the sample area under this direct observed image and the positional information of residing focusing aspect;
Said input equipment comprises the first input subset and the second input subset; Confirm to be used in the station location marker information of the direct observed image that current needs show to identify the positional information of the sample area under this direct observed image according to the input of the said first input subset, with simulation microscope stage mobile on the x-y plane; Confirm to be used to identify in the station location marker information of the direct observed image that current needs show the positional information of the residing focusing aspect of this direct observed image according to the input of the said second input subset, with simulation microscope stage mobile on the z axle.
21. method according to claim 20; It is characterized in that; The said first input subset comprise the four direction key of computer keyboard, the input of said four direction key be respectively applied for the simulation microscope stage on the x-y plane along laterally left and right sides both direction and vertically moving of former and later two directions.
22. method according to claim 20 is characterized in that, the said second input subset comprises the roller of computer mouse, and the scroll forward and backward of said roller is used to simulate microscope stage moving up and down along the z axle.
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| CN107561078A (en) * | 2017-10-17 | 2018-01-09 | 北京和众视野科技有限公司 | Textile fiber detection method |
| CN113418919A (en) * | 2021-04-30 | 2021-09-21 | 深圳市菲雀兰博科技研究中心有限公司 | Textile fiber component qualitative and quantitative online analysis system and method |
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