CN102623874A - Laser source device for coherent Raman scattering microscope system and production method of laser source device - Google Patents
Laser source device for coherent Raman scattering microscope system and production method of laser source device Download PDFInfo
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- 238000001069 Raman spectroscopy Methods 0.000 title claims abstract description 50
- 230000001427 coherent effect Effects 0.000 title claims abstract description 47
- 238000004519 manufacturing process Methods 0.000 title description 2
- 239000013078 crystal Substances 0.000 claims abstract description 71
- 230000003287 optical effect Effects 0.000 claims abstract description 32
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- 230000010287 polarization Effects 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 19
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- CPLXHLVBOLITMK-UHFFFAOYSA-N magnesium oxide Inorganic materials [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 claims description 15
- 239000000395 magnesium oxide Substances 0.000 claims description 15
- GQYHUHYESMUTHG-UHFFFAOYSA-N lithium niobate Chemical compound [Li+].[O-][Nb](=O)=O GQYHUHYESMUTHG-UHFFFAOYSA-N 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 9
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- WSMQKESQZFQMFW-UHFFFAOYSA-N 5-methyl-pyrazole-3-carboxylic acid Chemical compound CC1=CC(C(O)=O)=NN1 WSMQKESQZFQMFW-UHFFFAOYSA-N 0.000 claims description 6
- VCZFPTGOQQOZGI-UHFFFAOYSA-N lithium bis(oxoboranyloxy)borinate Chemical compound [Li+].[O-]B(OB=O)OB=O VCZFPTGOQQOZGI-UHFFFAOYSA-N 0.000 claims description 6
- AXZKOIWUVFPNLO-UHFFFAOYSA-N magnesium;oxygen(2-) Chemical compound [O-2].[Mg+2] AXZKOIWUVFPNLO-UHFFFAOYSA-N 0.000 claims description 5
- 230000001360 synchronised effect Effects 0.000 description 7
- 230000005284 excitation Effects 0.000 description 6
- 238000003384 imaging method Methods 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000001530 Raman microscopy Methods 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
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- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
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- 238000002834 transmittance Methods 0.000 description 1
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Abstract
The invention relates to a laser source device for a coherent Raman scattering microscope system. The laser source device comprises an ultrashort pulse fiber laser, a light beam splitter, a tunable continuous light semiconductor laser, an optical fiber amplifier, a first light beam combiner, a first nonlinear crystal, a second nonlinear crystal, a second light beam combiner, an optical delay line and a polarizer, wherein two split light beams are respectively used as Stokes light in a coherent Raman scattering process and pump light of a parametric amplifier; the optical fiber amplifier is used for amplifying output light of the continuous light semiconductor laser; the first light beam combiner is used for combining the pump light and seed light of the parametric amplifier in a space; the first nonlinear crystal and the second nonlinear crystal are used for sequentially receiving the light beam of the first light beam combiner, and the two nonlinear crystals are respectively used as a parametric amplifier and a frequency doubler; the second light beam combiner is used for combining the Stokes light and the output light of the frequency doubler in a space; the optical delay line is arranged in the light path of the Stokes light and used for carrying out light path adjustment on the Stokes light before the light beam combination of the second light beam combiner; and the polarizer is used for adjusting the polarization state.
Description
Technical Field
The invention belongs to the field of nonlinear optical microscopy, and particularly relates to a laser light source device of a coherent Raman scattering microscopy system and a generation method thereof.
Background
Coherent Raman Scattering microscopy comprises Coherent anti-Stokes Raman Scattering (CARS) microscopy and Stimulated Raman Scattering (SRS) microscopy, has the advantages of no need of labeling, high sensitivity, realization of three-dimensional imaging and the like, and has potential application in the field of real-time biomedical imaging.
However, the advantages of coherent raman microscopy come at the expense of its complex excitation light source. In order to realize coherent Raman scattering microscopic imaging, two beams of ultrashort light pulses with time synchronization, tunable wavelength and high energy are needed for excitation. When the wavelength difference of the two beams of excitation light is coincident with the characteristic Raman peak of a certain substance in the biological sample, the Raman scattering signal is greatly enhanced, and a coherent Raman scattering signal is generated. In practice, factors such as sample penetration depth, system transmittance and system complexity are comprehensively considered, generally, the Stokes light is selected to be near 1040nm, and the wavelength of the pump light is selected to be adjustable in the range of 690 nm-990 nm.
Advances in coherent raman microscopy are limited by the development of excitation light sources, and relevant information can be found in the literature "coherent stokes raman scattering microscopy: biological and medical chemical imaging (Conor L. Evansand X. Sun Xie, Coherent anti-Stokes Raman scattering microscopy: chemical imaging for biology and medicine, Annu. Rev. anal. chem., 1: 883909 (2008)) ". How to solve the synchronization problem between two beams of excitation pulses influences the system cost and complexity. At first, people adopt a scheme of locking two solid lasers in phase to obtain synchronous laser pulses, but a feedback control circuit of the synchronous laser pulses is very complex and expensive in manufacturing cost. Subsequently, a scheme of a solid laser and a synchronous pumped optical parametric oscillator thereof appears, and a synchronous pulse can be directly obtained, but the optical parametric oscillator still needs a feedback control circuit, so that the system structure is complex and the cost is high. Furthermore, Time lens (Time lens) techniques are also used to obtain the synchronization pulse, but they also require complex feedback control circuits and the resulting pulse train has a large noise floor. Therefore, the above solutions fail to effectively reduce the system cost and complexity.
Chao-Yu Chung et al proposed a scheme for obtaining the excitation pulse of the CARS microscope system with a solid-state laser and its pumped parametric amplifier (Chao-Yu Chung, Yen-Yin Lin, Kuo-YuWu, Wan-Yu Tai, Shi-Wei Chu, Yao-Chang Lee, Yeukuang Hwu, Yin-Yu Lee, Coherent anti-Stokes Raman scattering microscopic using a single-wavelength-scattering-optical fibers amplifier, Opt Express18(6), 6116-. In the scheme, a part of near infrared light (1064nm) pulse generated by a solid laser is coupled into a photonic crystal fiber to generate a supercontinuum, so that seed light (about 800nm) is provided for a parametric amplifier. The remaining part is frequency-doubled and used as pump light (532nm) of the parametric amplifier. In the above process, the generated new wavelength pulse (about 800nm) is automatically synchronous with the original near infrared pulse (1064nm), so that a feedback control circuit is not needed. However, because the power spectral density of the supercontinuum is small, when the supercontinuum is used as a seed of the parametric amplifier, the conversion efficiency of the parametric process is low, and the output power of the parametric amplifier is reduced. In addition, the system adopts a solid laser to generate near infrared pulses (1064nm), and has a complex structure and high price.
Therefore, a technical problem to be solved urgently by those skilled in the art is to provide an effective measure for providing a compact and low-cost laser light source for coherent raman scattering microscopy.
Disclosure of Invention
The invention provides a laser light source device of a coherent Raman scattering microscopy system, which aims to solve the problems of the conventional laser light source of the Raman scattering microscopy. The invention also provides a method for generating the laser light source of the coherent Raman scattering microscopy system.
In order to solve the above problems, the present invention discloses a laser light source device of a coherent raman scattering microscopy system, comprising:
the system comprises an ultrashort pulse fiber laser and a beam splitter for splitting output light of the ultrashort pulse fiber laser, wherein two beams of split light are respectively used as Stokes light in a coherent Raman scattering process and pump light of a parametric amplifier;
the tunable continuous optical semiconductor laser and the optical fiber amplifier for amplifying the output light of the tunable continuous optical semiconductor laser, wherein the amplified light is called seed light of the parametric amplifier;
the first beam combiner is used for spatially combining the pump light and the seed light of the parametric amplifier;
the first nonlinear crystal and the second nonlinear crystal sequentially receive the light beam of the first light beam combiner, and the two nonlinear crystals are respectively used as a parametric amplifier and a frequency multiplier;
a second beam combiner for spatially combining the Stokes light and the output light of the frequency multiplier;
and the optical delay line and the polarizer are arranged in the optical path of the Stokes light and respectively adjust the optical path length of the Stokes light before the beams are combined by the second beam combiner.
Optionally, the ultrashort pulse fiber laser is a femtosecond pulse fiber laser or a picosecond pulse fiber laser.
Optionally, the ultrashort pulse fiber laser is an ultrashort pulse ytterbium-doped fiber laser or an ultrashort pulse neodymium-doped fiber laser.
Optionally, the energy splitting ratio of the beam splitter is 1: 3, wherein a quarter energy beam is used as stokes light, and a three quarter energy beam is used as pump light.
Optionally, the output wavelength range of the tunable continuous optical semiconductor laser is 1510nm to 1640 nm.
Optionally, the optical fiber amplifier is an erbium-doped optical fiber amplifier.
Optionally, the polarization state of the seed light and the polarization state of the pump light before the beam combination are adjusted to be parallel by the two half-wave plates and focused by the two focusing lenses.
Optionally, the first nonlinear crystal is one of a lithium triborate crystal, a periodically poled lithium niobate crystal, a periodically poled magnesium oxide-doped lithium niobate crystal, and a periodically poled superlattice lithium tantalate crystal;
the second nonlinear crystal is one of a lithium triborate crystal, a periodically poled lithium niobate crystal, a periodically poled magnesium oxide doped lithium niobate crystal and a periodically poled superlattice lithium tantalate crystal.
Optionally, the first nonlinear crystal and the second nonlinear crystal are both disposed in a temperature-controlled furnace.
Optionally, a band-pass filter and a third focusing lens are sequentially arranged between the first nonlinear crystal and the second nonlinear crystal; a dichroic mirror is arranged between the second nonlinear crystal and the second beam combiner; wherein,
the band-pass filter is used for filtering out the pulse light with the new converted wavelength obtained by the parametric amplifier and projecting the pulse light to the third focusing lens;
and the dichroic mirror is used for filtering out the pulse light after frequency multiplication of the frequency multiplier and projecting the pulse light to the second light beam combiner.
Optionally, the polarizer is a half-wave plate, and is configured to adjust the polarization state of the stokes light to be parallel to the output light of the second nonlinear crystal.
The invention also provides a method for generating a laser light source of coherent Raman scattering microscopy, which comprises the following steps:
splitting output light of the ultrashort pulse fiber laser, wherein one part of the output light is used for providing Stokes light required by a coherent Raman scattering process, and the other part of the output light is used as pump light of a parametric amplifier;
amplifying the output light of the tunable continuous optical semiconductor laser through an optical fiber amplifier, and focusing the output light and the pump light of the split parametric amplifier to a first nonlinear crystal for parametric amplification;
filtering out the output pulse obtained after parametric amplification, and focusing the output pulse to a second nonlinear crystal for frequency doubling;
filtering out the pulsed light obtained by frequency doubling;
adjusting the optical path and the polarization state of Stokes light to ensure that the Stokes light and the frequency-doubled light pulse are coincided in time and the polarization states are parallel; and make the two coincide in space. Thus, the laser light source of the coherent Raman scattering microscope system can be obtained.
Compared with the prior art, the laser light source device of the coherent Raman scattering microscope system adopts the tunable continuous optical semiconductor laser to provide seed light for the parametric amplifier, skillfully obtains synchronous pulses, and improves the conversion efficiency of the parametric process, thereby reducing the system cost and complexity; the ultrashort pulse fiber laser is adopted to generate Stokes light required by coherent Raman scattering and pump light of a parametric amplifier, so that the system is compact in structure and low in cost.
Drawings
FIG. 1 is a schematic diagram of one embodiment of a laser light source arrangement of a coherent Raman scattering microscopy system of the present invention;
FIG. 2 is a temperature tuning curve of MgO: PPLN of the parametric amplifier in different polarization periods;
FIG. 3 is a temperature tuning curve of frequency doubling crystal MgO: PPLN under different polarization periods.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
Fig. 1 is a schematic diagram of one embodiment of a laser light source device of a coherent raman scattering microscopy system according to the present invention.
Referring to fig. 1, the apparatus of the present embodiment includes an ultrashort pulse fiber laser 1-1 for generating stokes light required for coherent raman scattering process and pump light of a parametric amplifier.
And the beam splitter 2 is used for splitting the output light of the ultrashort pulse fiber laser 1-1 according to a certain power ratio to generate the Stokes light and the pump light.
And the optical delay line 3 is used for adjusting or compensating the optical path difference. A polarizer 18 for adjusting the polarization state.
The first beam combiner 10 and the second beam combiner 5 are used for combining two beams of light with different wavelengths in space;
and the tunable continuous optical semiconductor laser 1-2 is used for obtaining seed light of the parametric amplifier. And the optical fiber amplifier 1-3 is used for amplifying the output of the tunable continuous optical semiconductor laser 1-2, and the amplified light is called seed light of the parametric amplifier.
The first nonlinear crystal 12 and the second nonlinear crystal 16 are respectively used for parametric amplification and frequency doubling.
When a specific optical path is formed, the apparatus of this embodiment further includes the following components:
a bandpass filter 13 filters out the pulses of the new converted wavelength obtained by the parametric amplifier.
A first half-wave plate 1 (wherein the first and second are used herein only for distinguishing devices having the same name, and do not represent ordinal numbers), and a second half-wave plate 8 for adjusting the polarization state of light.
A dichroic mirror 17 is used for filtering.
A first lens 6, a second lens 9 and a focusing lens 14 for focusing the light beam.
And the optical fiber collimator 7 is used for collimating and outputting the output of the erbium-doped optical fiber amplifier 1-3.
And a reflecting mirror 4 for reflecting the light path.
A first temperature controlled furnace 11 and a second temperature controlled furnace 15 for adjusting and maintaining the first nonlinear crystal 12 and the second nonlinear crystal 16 at proper working temperatures.
The following description will be given with reference to specific examples.
The ultrashort pulse fiber laser 1-1 is an ultrashort pulse ytterbium-doped fiber laser (or an ultrashort pulse neodymium-doped fiber laser) in the embodiment, and can obtain output light with a central wavelength of 1040nm, a full width at half maximum of 3ps and pulse energy of 10 nJ. The optical fiber is split by a beam splitter 2 (splitting ratio is 1: 3), wherein a part with pulse energy of 7.5nJ is used as pump light of a parametric amplifier, and another part with pulse energy of 2.5nJ is used as Stokes light required by a coherent Raman scattering process. The ultrashort pulse fiber laser 1-1 can also be a femtosecond laser or a picosecond laser with other wave bands. It should be noted that the beam splitter 2 may have other splitting ratios, and is not limited to 1: 3. The beam splitter with any beam splitting ratio can be selected according to actual needs.
The tunable continuous optical semiconductor laser 1-2 is a laser with an output wavelength of 1510-. The optical fiber amplifiers 1 to 3 are erbium-doped optical fiber amplifiers, and the amplified light is collimated by the optical fiber collimator 7 and output as seed light of a subsequent parametric amplifier.
The first half-wave plate 1 and the second half-wave plate 8 respectively adjust the polarization states of the pump light and the seed light to be parallel, the first lens 6 and the second lens 9 are adopted for focusing, and then the pump light and the seed light are combined to a first nonlinear crystal 12 through a first beam combiner 10, and the first nonlinear crystal 12 is used as a parametric amplifier. The first nonlinear crystal 12 may be one of a lithium triborate LBO crystal, a periodically poled lithium niobate PPLN crystal, a periodically poled magnesium oxide doped lithium niobate MgO, a PPLN crystal, and a periodically poled superlattice lithium tantalate PPLST crystal. Specifically, in this embodiment, the multi-polarization period MgO: PPLN crystal (the polarization period includes 29.6um, 30um, and 30.4um) is obtained by selecting a waveguide with a desired polarization period and adjusting the first temperature controlled oven 11 to a proper temperature for parametric amplification (the temperature tuning curves of the parametric amplifier MgO: PPLN in different polarization periods are shown in fig. 2).
The 1510-1640nm output pulses from the parametric amplifier are filtered out by a bandpass filter 13 and focused by a focusing lens 14 onto a second nonlinear crystal 16, which second nonlinear crystal 16 acts as a frequency multiplier. The second nonlinear crystal 16 can be one of a lithium triborate LBO crystal, a periodically poled lithium niobate PPLN crystal, a periodically poled magnesium oxide doped lithium niobate MgO, a PPLN crystal, and a periodically poled superlattice lithium tantalate PPLST crystal. Specifically, in this embodiment, the multi-polarization period MgO: PPLN crystal (the polarization period includes 18.2um, 18.8um, 19.4um, 20.0um, 20.6um, and 21.2um), selects the waveguide with the desired polarization period and adjusts the second temperature-controlled oven 15 to the proper temperature for frequency doubling (the temperature tuning curve of the frequency doubling crystal MgO: PPLN at different polarization periods is shown in fig. 3).
The 755-820nm pulsed light obtained after frequency doubling by the frequency multiplier is filtered out by the dichroic mirror 17. The optical path length of the stokes light is adjusted by the optical delay line 3, and the two pulses are temporally overlapped. And a polarizer 18 (a half-wave plate in this embodiment) in the stokes path is adjusted so that the polarization states of the two pulses are parallel. And the light filtered by the dichroic mirror 17 is spatially coincident with the stokes light using the second beam combiner 5. After being coupled, the two pulses can be used as a laser light source of coherent Raman scattering microscopy, for example, the two pulses are coupled to a microscope 1-5 to carry out CARS microscopic imaging.
For SRS microscopic imaging, an acousto-optic modulator 19 (or an electro-optic modulator) is also used to modulate the intensity of stokes light for correlation detection. The acousto-optic modulator 19 is controlled via a signal processing unit 1-7 and a signal generator connected to the computer 1-6. And will not be described in detail herein.
The output wavelength (in the range of 1510 to 1640nm) of the tunable continuous optical semiconductor laser is tuned, and the device can realize a Raman characteristic peak of 2579cm-1To 3846cm-1The range of samples was subjected to coherent raman scattering microscopy.
For example, the content of oil in a biological sample is detected (wherein the characteristic peak of CH2 bond is 2845 cm)-1Near) the output wavelength of the tunable continuous optical semiconductor laser may be set at 1605 nm. Referring to FIG. 2, a MgO PPLN crystal 12 waveguide with a polarization period of 30.4um can be selected and set at 120 deg.C, where the parametric amplifier can dissipate 1040nm pulses and obtain 1605nm pulses. Referring to FIG. 3, a MgO PPLN crystal 16 waveguide with a polarization period of 20.6um is selected and set at a temperature of 102.8 deg.C, and then 1605nm pulses are frequency-doubled to obtain 802.5nm pulses. The 1040nm pulse obtained by beam splitting and the newly generated 802.5nm pulse are overlapped in time and space, and the method can be used for CARS microscopic imaging of grease.
Therefore, the device of the above embodiment of the present invention can automatically obtain the synchronization pulse at the seed light wavelength because the pulse pump light in the parametric amplifier performs parametric amplification on the continuous seed light, so that the synchronization problem between the two beams of light does not need to be considered. And high parameter conversion efficiency can be obtained due to the high power spectral density of the continuous seed light.
According to the device provided by the embodiment of the invention, the tunable continuous optical semiconductor laser is adopted to provide seed light for the parametric amplifier, so that synchronous pulses can be skillfully obtained, the conversion efficiency of a parametric process is improved, and the cost and the complexity of a system are reduced; the system adopts the ultrashort pulse fiber laser to generate Stokes light required by coherent Raman scattering and pump light of the parametric amplifier, and has compact structure and low cost.
Although the present invention has been described with reference to the preferred embodiments, it is not intended to be limited thereto, and variations and modifications may be made by those skilled in the art without departing from the spirit and scope of the present invention.
Claims (12)
1. A laser light source device for a coherent raman scattering microscopy system, comprising:
the system comprises an ultrashort pulse fiber laser and a beam splitter for splitting output light of the ultrashort pulse fiber laser, wherein two beams of split light are respectively used as Stokes light in a coherent Raman scattering process and pump light of a parametric amplifier;
the tunable continuous optical semiconductor laser and the optical fiber amplifier for amplifying the output light of the tunable continuous optical semiconductor laser, wherein the amplified light is called seed light of the parametric amplifier;
the first beam combiner is used for spatially combining the pump light and the seed light of the parametric amplifier;
the first nonlinear crystal and the second nonlinear crystal sequentially receive the light beam of the first light beam combiner, and the two nonlinear crystals are respectively used as a parametric amplifier and a frequency multiplier;
a second beam combiner for spatially combining the Stokes light and the output light of the frequency multiplier;
and the optical delay line and the polarizer are arranged in the optical path of the Stokes light and respectively adjust the optical path length of the Stokes light before the beams are combined by the second beam combiner.
2. The laser light source apparatus of a coherent raman scattering microscopy system according to claim 1, wherein: the ultrashort pulse fiber laser is a femtosecond pulse fiber laser or a picosecond pulse fiber laser.
3. The laser light source apparatus of a coherent raman scattering microscopy system according to claim 1, wherein: the ultrashort pulse fiber laser is an ultrashort pulse ytterbium-doped fiber laser or an ultrashort pulse neodymium-doped fiber laser.
4. The laser light source apparatus of a coherent raman scattering microscopy system according to claim 1, wherein: the energy splitting ratio of the beam splitter is 1: 3, wherein one quarter of the energy beam is used as Stokes light in a coherent Raman scattering process, and three quarters of the energy beam is used as pump light of the parametric amplifier.
5. The laser light source apparatus of a coherent raman scattering microscopy system according to claim 1, wherein: the output wavelength range of the tunable continuous optical semiconductor laser is 1510nm to 1640 nm.
6. The laser light source apparatus of a coherent raman scattering microscopy system according to claim 5, wherein: the optical fiber amplifier is an erbium-doped optical fiber amplifier.
7. The laser light source device of the coherent raman scattering microscopy system according to claim 6, wherein: the seed light and the pump light are respectively subjected to polarization state adjustment to be parallel through the two half wave plates before being combined, and are focused through the two focusing lenses.
8. The laser light source apparatus of a coherent raman scattering microscopy system according to claim 1, wherein: the first nonlinear crystal is one of a lithium triborate crystal, a periodically poled lithium niobate crystal, a periodically poled magnesium oxide doped lithium niobate crystal and a periodically poled superlattice lithium tantalate crystal;
the second nonlinear crystal is one of a lithium triborate crystal, a periodically poled lithium niobate crystal, a periodically poled magnesium oxide doped lithium niobate crystal and a periodically poled superlattice lithium tantalate crystal.
9. The laser light source device of the coherent raman scattering microscopy system according to claim 8, wherein: and the first nonlinear crystal and the second nonlinear crystal are both arranged in a temperature control furnace.
10. The laser light source device of the coherent raman scattering microscopy system according to claim 1, wherein: a band-pass filter and a third focusing lens are sequentially arranged between the first nonlinear crystal and the second nonlinear crystal; a dichroic mirror is arranged between the second nonlinear crystal and the second beam combiner; wherein,
the band-pass filter is used for filtering out the pulse light with the new converted wavelength obtained by the parametric amplifier and projecting the pulse light to the third focusing lens;
and the dichroic mirror is used for filtering out the pulse light after frequency multiplication of the frequency multiplier and projecting the pulse light to the second light beam combiner.
11. The laser light source device of the coherent raman scattering microscopy system according to claim 1, wherein: the polarizer is a half wave plate and is used for adjusting the polarization state of the Stokes light to be parallel to the output light of the second nonlinear crystal.
12. A method of generating a laser light source for a coherent raman scattering microscopy system, comprising:
splitting output light of the ultrashort pulse fiber laser, wherein one part of the output light is used for providing Stokes light required by a coherent Raman scattering process, and the other part of the output light is used as pump light of a parametric amplifier;
amplifying the output light of the tunable continuous optical semiconductor laser through an optical fiber amplifier, and focusing the output light and the pump light of the split parametric amplifier to a first nonlinear crystal for parametric amplification;
filtering out the output pulse obtained after parametric amplification, and focusing the output pulse to a second nonlinear crystal for frequency doubling;
filtering out the pulsed light obtained by frequency doubling;
adjusting the optical path and the polarization state of Stokes light to ensure that the Stokes light and the frequency-doubled light pulse are coincided in time and the polarization states are parallel; and make the two coincide in space. Thus, the laser light source of the coherent Raman scattering microscope system can be obtained.
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