CN102618532A - Kit for extracting genome DNA (Deoxyribose Nucleic Acid) from plant leaves based on paramagnetic particle method and extracting method thereof - Google Patents
Kit for extracting genome DNA (Deoxyribose Nucleic Acid) from plant leaves based on paramagnetic particle method and extracting method thereof Download PDFInfo
- Publication number
- CN102618532A CN102618532A CN2012101313235A CN201210131323A CN102618532A CN 102618532 A CN102618532 A CN 102618532A CN 2012101313235 A CN2012101313235 A CN 2012101313235A CN 201210131323 A CN201210131323 A CN 201210131323A CN 102618532 A CN102618532 A CN 102618532A
- Authority
- CN
- China
- Prior art keywords
- plant leaf
- extracting
- centrifuge tube
- genomic dna
- magnetic force
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention belongs to the technical field of molecular biology, and particularly relates to a kit for extracting genome DNA (Deoxyribose Nucleic Acid) from plant leaves based on a paramagnetic particle method and an extracting method thereof. The kit for extracting genome DNA from plant leaves based on the paramagnetic particle method comprises a pretreatment solution, a rapid cracking liquid, a DNA binding liquid, a magnetic bead suspension and an eluent, wherein cell walls and cell membranes can be effectively cracked through the pretreatment solution; and the interference of impurities such as polysaccharides, polyphenol, tannin and the like on genome DAN extraction can be effectively eliminated through the pretreatment solution. In the rapid cracking liquid, guanidine hydrochloride and Tween 20 are taken as main components, so that plant cells can be fully cracked at the normal temperature within 2 minutes, only two minutes are required in a subsequent centrifuging step, and the operation time is greatly saved. The guanidine hydrochloride is a strong denaturant, has a good cell cracking effect. Moreover, organic solvents such as chloroform and the like are not required to be added, so that damages to operating personnel are avoided, and safety and reliability are achieved.
Description
Technical field
The invention belongs to technical field of molecular biology, relate in particular to the test kit and the process for extracting thereof that from plant leaf, extract genomic dna based on paramagnetic particle method.
Background technology
Along with molecular biology and biomedical continuous development, genomic dna becomes an important research project, and genomic dna plays control action kou to vital processes such as the growth of biology, heredity, variations.To the research of genomic dna, at first relate to the extraction of genomic dna.
For plant, the key step of extracting genomic dna is: broken wall, rupture of membranes, removal impurity, rinsing and wash-out.At first, in the middle of the process of extracting, use decontamination reagents such as SDS or CTAB can destroy cytolemma, intracellular protein precipitation is got off, thereby intracellular genomic dna is discharged; Secondly, utilize the extractive method of sedimentary method of high salt or chloroform phenol to remove impurity such as protein, polyphenol and polysaccharide; Then, in the supernatant that extracts, add an amount of volume of ethanol, Virahol and PEG equal solvent, make genomic dna be adsorbed on the media such as silicagel column, silica gel magnetic bead and granulated glass sphere ion exchange column; Adding rinsing liquid again and carry out rinsing, is to add elutriant genomic dna is eluted from medium at last.
At present, the adsorption medium that generally uses of nucleic acid purification is a silicagel column on the market, and the solid dielectric that silicagel column is taked is fibrous pellosil, and staple all is a silicon-dioxide.Ultimate principle is: nucleic acid is at high salt, low temperature, and (can not be lower than 4.0) can optionally combine with silicagel column or magnetic bead under the ethanol of low PH or suitable concn and the situation of Virahol; And at high temperature; Less salt, under the situation of high PH, can be from the silicagel column or magnetic bead eluted.Under most of situation, the combining of nucleic acid and silicagel column need add a certain amount of ethanol or Virahol, and fragment is more little, and the amount of adding is big more, and adsorption rate is not high.
The process for extracting of plant genome DNA generally has CTAB method, SDS method and high salt method.
The CTAB method has good clean effect, because CTAB is a kind of stain remover, under the situation of heating, ability is the cracking vegetable cell effectively, and CTAB can also combine with carbohydrate simultaneously, and through centrifugation polysaccharide is removed.The CTAB method also is applied to the extraction of various plants genomic dna, but at present general CTAB method test kit uses complex operation, and, generally can be used with deleterious organic solution such as chloroform phenol.
The SDS method is the traditional method of extracting genome DNA, not only is used for the extraction of plant genome DNA, also is used for the extraction of other tissue DNAs, such as cell, and animal tissues, blood.SDS is a kind of AS, under the situation of heating, can cracking grinds vegetable cell later, and SDS can also with the K ionic reaction, produce white flocculent substance, this material can with protein binding, through centrifugal coprecipitation.But the SDS method also has certain defective, and the time that in the middle of cracking and the process left, consumes is longer.
A kind of process for extracting-CTAB method of plant genome DNA of the prior art is provided, and it comprises the steps:
Supernatant is removed in step 6, suction, adds 70% ethanol, centrifuge tube lower magnetic force frame, and fully the vortex mixing leaves standstill 1min, magnetic force frame on the centrifuge tube;
Step 9, adding 100-150 μ L preheating (65 ℃) elutriant.Centrifuge tube lower magnetic force frame, fully the vortex mixing carries out water-bath 5min in 65 ℃ of water;
Step 10, centrifuge tube place on the magnetic force frame, leave standstill 2min;
Step 11, carefully with the supernatant sucking-off, subsequent use.
Still there are some shortcomings in this technical scheme:
1, versatility is not strong.For extracting paddy rice; The genomic dna of common plant such as wheat is respond well, but for extracting cotton, grape; In the time of the genomic dna of the plant leaf tissue that secondary metabolite contents such as these polysaccharide of black currant, polyphenol, tannin, lipid are abundant; Its purifying success ratio is lower, can't thoroughly remove polyphenol, polyphenols.Materials such as polyphenol has oxidation and produces brown stain in the process of extracting, polysaccharide with can generation heavy-gravity jelly after genomic dna combines, these 2 kinds of situation all cause genomic dna to degrade easily, the genomic dna purity that causes extracting is not high.
2, the leaf tissue of lysate after cracking is ground need need centrifugation time 10min simultaneously at 60 ℃ of heating in water bath 30min, and the time that these steps consume is too much.
When 3, extracting the genomic dna of the abundant plant leaf tissue of secondary metabolite content such as cotton, need the deleterious organic solvent chloroform of adding etc., healthy unfavorable for operator, produced simultaneously waste liquid also can pollute.
Summary of the invention
The objective of the invention is to provide a kind of cotton that goes for, grape, plants such as black currant from plant leaf, extract the test kit and the process for extracting thereof of genomic dna based on paramagnetic particle method.
According to an aspect of the present invention, what provide extracts the test kit of genomic dna based on paramagnetic particle method from plant leaf, and it comprises: preprocessing solution, and lysate fast, DNA combines liquid, bead suspension and elutriant,
Preprocessing solution: 0.05M～0.2M Tris-Hcl, 0.01～0.05M EDTA (YD 30), volume ratio be 1%～3% mercaptoethanol, mass/volume than the PVP (Vinylpyrrolidone polymer) that is 0.5%～2%,
Quick lysate: 50～100mmol/L KAC (Potassium ethanoate); PH=5.2,10～50mmol/LEDTA (YD 30), 4～6M GHCL (Guanidinium hydrochloride), 5～25mM sodium citrate (Trisodium Citrate), 1%～5%Tween 20 (Tween 20)
DNA combines liquid: mass/volume is than the PEGS000 (polyoxyethylene glycol 8000), the 1.5-4M GITC (guanidinium isothiocyanate) that are 5%～10%, 0.5-1M NaCl,
Bead suspension: magnetic bead is dissolved in the ultrapure water, and wherein the concentration of magnetic bead is 50-100mg/mL,
Elutriant: 1～10mM Tris-HCl, 1-2mM EDTA (YD 30), pH=8.0.
Its beneficial effect is; Preprocessing solution can effectively crack cell walls and cytolemma; But can not destroy nucleus, because genomic dna is positioned at nucleus, polysaccharide, polyphenol, tannin etc. then are present in the tenuigenin; Because polysaccharide, polyphenol, tannin can be dissolved in the preprocessing solution, so can effectively remove the interference of impurity such as polysaccharide, polyphenol, tannin for extracting genome DNA through preprocessing solution.
Lysate is a staple with Guanidinium hydrochloride and Tween 20, can be in 2min under the normal temperature condition abundant cracking vegetable cell, and follow-up centrifugation step is as long as 2min has practiced thrift the running time greatly.Guanidinium hydrochloride is a strong denaturant, and the effect of its lysing cell is much better than CTAB and SDS.
Adopt the 5%PEG of 2 times of volumes, 4M GITC and 0.5M NaCl solution replace absolute ethyl alcohol to combine liquid as DNA, better effects if, and yield is higher.
And, in the middle of the technical scheme that the present invention adopts, need not add organic solvent chloroform etc., can not produce harm to operator, safe and reliable.
In some embodiments, the particle diameter of magnetic bead is 100-200nm, and magnetic bead surfaces encapsulates the silicon hydroxyl.
Its beneficial effect is through magnetic bead genomic dna to be produced adsorption, thereby realize the extraction operation to genomic dna; Because the magnetic bead outside surface of Nano grade is a layer of silicon dioxide; Because magnetic bead particles is little, specific surface area is big, and the yield of paramagnetic particle method generally all will be higher than the silica gel column method; What is more important, magnetic bead side carries out robotization and high-throughput more easily.
According to a further aspect in the invention, what provide extracts the process for extracting of the test kit of genomic dna based on paramagnetic particle method from plant leaf, and it comprises:
Supernatant is removed in step 7, suction, and the adding volume ratio is 70% ethanol, and centrifuge tube is taken off from the magnetic force frame, fully leaves standstill 1-3min behind the vortex mixing, centrifuge tube is placed on the magnetic force frame again;
Step 9, the DNA that adds 65 ℃ of 100-150 μ L preheatings combine liquid, centrifuge tube is taken off from the magnetic force frame, abundant vortex mixing, and in 55-80 ℃ of water water-bath 2-8min;
Step 10, centrifuge tube is placed on the magnetic force frame, leave standstill 1-2min;
Step 11, carefully with the supernatant sucking-off, subsequent use.
Its beneficial effect is that process for extracting of the present invention is particularly useful for cotton, grape, the extracting genome DNA of the plant leaf tissue that secondary metabolite contents such as these polysaccharide of black currant, polyphenol, tannin, lipid are abundant.
In some embodiments, in step 1,
If the plant leaf of getting is the fresh plant leaf, then the amount of getting is no more than 100mg,
If institute's plant leaf of getting is dry tissue, then the amount of getting is no more than 50mg.
Its beneficial effect is, because water content is more in the fresh plant leaf, so the amount of getting is slightly larger, and the moisture content less of dry tissue, so the amount of getting can be slightly smaller.
In some embodiments, in step 1, in mortar, grind again behind the adding liquid nitrogen.
Its beneficial effect is to utilize liquid nitrogen to create a low temperature environment, on the one hand; Low temperature can suppress the activity of nucleicacidase, makes genomic dna avoid the invasion and attack of nucleicacidase, on the other hand; Low temperature can make the fragility of object strengthen, thereby is more convenient for grinding, and also more laborsaving when grinding.
In some embodiments, between step 7 and step 8, also comprise a step, inhale and remove supernatant that adding volume ratio is 70% ethanol, centrifuge tube is taken off from the magnetic force frame, fully leave standstill 1-3min behind the vortex mixing, again centrifuge tube is placed on the magnetic force frame.
Its beneficial effect is that through twice same repetitive operation, the purity of the feasible genomic dna that extracts is more high.
Description of drawings
Fig. 1 is the agarose electrophoresis comparison diagram of the genomic dna of test kit of the present invention and process for extracting extraction thereof; Wherein the M road is the mark swimming lane, the fresh tender leaf genomic dna of the cotton band that 1,2 swimming lane extracts for CTAB method among the experiment 1-1; 3; The cotton fresh tender leaf genomic dna band of 4 swimming lanes for adopting the present invention to extract among the experiment 1-1, the cotton Lao Ye genomic dna band that 5,6 swimming lanes extract for CTAB method among the experiment 1-2; The cotton Lao Ye genomic dna band of 7,8 swimming lanes for adopting the present invention to extract among the experiment 1-2.
Fig. 2 is the another agarose electrophoresis comparison diagram of the genomic dna of test kit of the present invention and process for extracting extraction thereof; Wherein, the rice leaf genomic dna band of 1,2 swimming lane for adopting the CTAB method to extract in the experiment 2; The rice leaf genomic dna band of 3,4 swimming lanes for adopting the present invention to extract in the experiment 2.
Embodiment
Come further to set forth the present invention below in conjunction with concrete embodiment, should be appreciated that these embodiment only are used to the present invention that explains, multi-form equivalence replacement can be arranged, present embodiment can not limit protection scope of the present invention.
1, test kit
A kind ofly from plant leaf, extract the test kit of genomic dna based on paramagnetic particle method, it comprises: preprocessing solution, and lysate fast, DNA combines liquid, bead suspension and elutriant,
Preprocessing solution comprises: 0.2M tris-HCL, 0.05M EDTA, volume ratio be 2% mercaptoethanol and mass/volume than the PVP that is 2%,
Lysate comprises fast: 100mmol/L KAC, and PH=5.2,50mmol/L EDTA, 6MGHCl, 5mM sodium citrate, 2%Tween 20,
DNA combines liquid to comprise: 5%PEG8000,4M GITC, and 0.5M NaCl,
Bead suspension comprises: magnetic bead is dissolved in the ultrapure water, and wherein the concentration of magnetic bead is 50mg/mL, and the particle diameter of magnetic bead is 100-200nm, and magnetic bead surfaces encapsulates the silicon hydroxyl,
Elutriant comprises: 5mM Tris-HCl, 1M EDTA, pH=8.0.
Wherein tris-HCL adopts the BB0079 of Canadian biotech company (BBI); EDTA adopts the E6758 of U.S. Sigma company (Sigma); PVP adopts the #PB0436 of Canadian biotech company (BBI); KAC adopts the PB0438 of Canadian biotech company (BBI), and GHCL adopts the #G3272 of U.S. Sigma company (Sigma), and sodium citrate adopts the #C8532 of U.S. Sigma company (Sigma); Tween 20 adopts the #P9416 of U.S. Sigma company (Sigma); PEG8000 adopts the #PB0433 of Canadian biotech company (BBI), and GITC adopts the #G9277 of U.S. Sigma company (Sigma), and NaCl adopts the ST1218 of Canadian biotech company (BBI).
2, genome DNA extracting method
Adopt this test kit to extract the method for genomic dna in the middle of the plant, its step comprises:
Supernatant is removed in step 7, suction, adds 70% ethanol, and centrifuge tube is taken off from the magnetic force frame, fully leaves standstill 1min behind the vortex mixing, centrifuge tube is placed on the magnetic force frame again;
Whole operations of step 8, repeating step seven once
Step 9, centrifuge tube is placed air-dry 10min on the magnetic force frame;
Step 10, add the DNA elutriant of 65 ℃ of 150 μ L preheatings, centrifuge tube taken off from the magnetic force frame, abundant vortex mixing, and in 65 ℃ of water water-bath 5min;
Step 11, centrifuge tube is placed on the magnetic force frame, leave standstill 2min;
Step 12, carefully with the supernatant sucking-off, subsequent use.
3, comparative analysis as a result
The experimental result of the CTAB method of the experimental result of technical scheme of the present invention and prior art is compared analysis.
Experiment 1-1
Select for use the fresh tender leaf of 100mg cotton to carry out the extraction of genomic dna.Adopt the CTAB method of process for extracting of the present invention and prior art to carry out simultaneously simultaneously.All use 150 μ L elutriants at last
It is as shown in the table in the data contrast of experimental result:
The image comparison of experimental result (2% agarose gel electrophoresis) as shown in Figure 1.
Experiment conclusion: the extraction effect of process for extracting of the present invention will obviously be better than the CTAB method of prior art.
Experiment 1-2
Select for use 50mg cotton Lao Ye to carry out the extraction of genomic dna.Adopt the CTAB method of process for extracting of the present invention and prior art to carry out simultaneously simultaneously.All use 150 μ L elutriant wash-outs at last.
It is as shown in the table in the data contrast of experimental result:
The image comparison of experimental result is as shown in Figure 1.
Experiment conclusion: the amount of the CTAB method extracting genome DNA of prior art seldom, purity is also very low, and process for extracting of the present invention still can extract more genomic dna, and purity is also high a lot of than CTAB method.
Select for use the fresh blade of 100mg paddy rice to carry out the extraction of genomic dna.Adopt the CTAB method of process for extracting of the present invention and prior art to carry out simultaneously simultaneously.All use 150 μ L elutriant wash-outs at last.
It is as shown in the table in the data contrast of experimental result:
The image comparison of experimental result is as shown in Figure 2.
Experiment conclusion: for plants such as paddy rice, the effect difference of two kinds of methods is little, but in time, it is many that the CTAB method of the time ratio prior art of wanting required for the present invention will be lacked.
The above only is a preferred implementation of the present invention; Should be pointed out that to those skilled in the art, under the prerequisite that does not break away from the invention design; Can also make some similar distortion and improvement, these also should be regarded as within protection scope of the present invention.
Claims (6)
1. from plant leaf, extract the test kit of genomic dna based on paramagnetic particle method, it comprises: preprocessing solution, and quick lysate, DNA combines liquid, bead suspension and elutriant,
Preprocessing solution: 0.05M～0.2M Tris-Hcl, 0.01～0.05M EDTA (YD 30), 1%～3% mercaptoethanol, 0.5%～2%PVP (Vinylpyrrolidone polymer),
Quick lysate: 50～100mmol/L KAC (Potassium ethanoate); PH=5.2,10～50mmol/LEDTA (YD 30), 4～6M GHCL (Guanidinium hydrochloride), 5～25mM sodium citrate (Trisodium Citrate), 1%～5%Tween 20 (Tween 20)
DNA combines liquid: 5%～10%PEG8000 (polyoxyethylene glycol 8000), 1.5-4M GITC (guanidinium isothiocyanate), 0.5-1M NaCl,
Bead suspension: magnetic bead is dissolved in the ultrapure water, and wherein the concentration of magnetic bead is 50-100mg/mL,
Elutriant: 1～10mM Tris-HCl, 1-2mM EDTA (YD 30), pH=8.0.
2. according to claim 1ly from plant leaf, extract the test kit of genomic dna based on paramagnetic particle method, wherein, the particle diameter of magnetic bead is 100-200nm, and magnetic bead surfaces encapsulates the silicon hydroxyl.
3. claim 1 or 2 is describedly extracted the process for extracting of the test kit of genomic dna based on paramagnetic particle method from plant leaf, and it comprises:
Step 1, get plant leaf and be put in the mortar and grind fully, milling time is 1-2min;
Step 2, if the plant leaf of getting be polysaccharide, polyphenol, tannin, lipid content abundant type plant leaf adds 500 μ L pretreatment fluids, be paddy rice as if the plant of getting, wheat or the simple plant leaf of corn class then directly get into step 3;
Step 3, the quick lysate of adding 500 μ L;
Step 4,8,000-12, centrifugal 1-3min under the 000rpm;
Step 5, carefully draw in the new 2mL centrifuge tube of 400 μ L supernatants to, avoid picking up deposition, add mixed solution 400-1000 μ L DNA and combine liquid and 10-50 μ L bead suspension, abundant vortex mixing;
Step 6, after leaving standstill 5-10min under 18-25 ℃, centrifuge tube is placed on the magnetic force frame, leave standstill 1-3min;
Supernatant is removed in step 7, suction, and the adding volume ratio is 70% ethanol, and centrifuge tube is taken off from the magnetic force frame, fully leaves standstill 1-3min behind the vortex mixing, centrifuge tube is placed on the magnetic force frame again;
Step 8, centrifuge tube is placed air-dry 5-15min on the magnetic force frame;
Step 9, the DNA that adds 65 ℃ of 100-150 μ L preheatings combine liquid, centrifuge tube is taken off from the magnetic force frame, abundant vortex mixing, and in 55-80 ℃ of water water-bath 2-8min;
Step 10, centrifuge tube is placed on the magnetic force frame, leave standstill 1-2min;
Step 11, carefully with the supernatant sucking-off, subsequent use.
4. according to claim 3ly from plant leaf, extract the process for extracting of genomic dna based on paramagnetic particle method, wherein: in the step 1,
If the plant leaf of getting is the fresh plant leaf, then the amount of getting is no more than 100mg,
If institute's plant leaf of getting is dry tissue, then the amount of getting is no more than 50mg.
5. according to claim 4ly from plant leaf, extract the process for extracting of genomic dna, wherein, in the step 1, grind again after in mortar, adding liquid nitrogen based on paramagnetic particle method.
6. according to claim 3 or the 4 or 5 described process for extracting that from plant leaf, extract genomic dna based on paramagnetic particle method; Wherein, also comprise a step between step 7 and the step 8, inhale and remove supernatant; The adding volume ratio is 70% ethanol; Centrifuge tube is taken off from the magnetic force frame, fully leave standstill 1-3min behind the vortex mixing, again centrifuge tube is placed on the magnetic force frame.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101313235A CN102618532A (en) | 2012-05-02 | 2012-05-02 | Kit for extracting genome DNA (Deoxyribose Nucleic Acid) from plant leaves based on paramagnetic particle method and extracting method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101313235A CN102618532A (en) | 2012-05-02 | 2012-05-02 | Kit for extracting genome DNA (Deoxyribose Nucleic Acid) from plant leaves based on paramagnetic particle method and extracting method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102618532A true CN102618532A (en) | 2012-08-01 |
Family
ID=46558743
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012101313235A Pending CN102618532A (en) | 2012-05-02 | 2012-05-02 | Kit for extracting genome DNA (Deoxyribose Nucleic Acid) from plant leaves based on paramagnetic particle method and extracting method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102618532A (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103898092A (en) * | 2014-01-27 | 2014-07-02 | 西安天隆科技有限公司 | Kit and method for extracting plant tissue genome DNA by adopting quick paramagnetic particle method |
| CN104630208A (en) * | 2015-02-11 | 2015-05-20 | 杭州百迈生物技术有限公司 | Kit and method for extracting genome DNA (deoxyribonucleic acid) |
| CN106119394A (en) * | 2016-08-26 | 2016-11-16 | 刘明涛 | The nucleotide sequence authentication method of a kind of generation latitude lettuce tongue and the application of nucleotide sequence |
| CN106480014A (en) * | 2015-09-02 | 2017-03-08 | 创想生物科技有限公司 | Method for selectively isolating nucleic acids and kit therefor |
| CN107267502A (en) * | 2017-08-10 | 2017-10-20 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A kind of high efficiency extraction sugarcane sugar prod DNA kit and method |
| CN109055363A (en) * | 2018-09-21 | 2018-12-21 | 武汉菲沙基因信息有限公司 | A kind of magnetic bead reagent and screening technique suitable for screening overlength nucleic acids |
| CN110358762A (en) * | 2019-07-26 | 2019-10-22 | 云南省农业科学院热区生态农业研究所 | A kind of kit and its extracting method extracting plant leaf blade genomic DNA |
| CN111197073A (en) * | 2019-12-19 | 2020-05-26 | 武汉艾米森生命科技有限公司 | Method for extracting DNA sample from excrement and methylation detection method of colorectal cancer related gene |
| CN112501155A (en) * | 2020-11-06 | 2021-03-16 | 南方医科大学顺德医院(佛山市顺德区第一人民医院) | DNA extraction kit applied to transgenic mouse gene identification |
| CN114015681A (en) * | 2021-11-15 | 2022-02-08 | 深圳市人民医院 | Improved extraction method of polysaccharide polyphenol plant CTAB |
| CN114426967A (en) * | 2020-10-29 | 2022-05-03 | 西南大学 | Non-toxic plant universal high-purity genome DNA rapid extraction method and kit |
| CN115873848A (en) * | 2022-12-24 | 2023-03-31 | 蓝景科信河北生物科技有限公司 | Plant DNA extraction kit |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000063362A1 (en) * | 1999-04-21 | 2000-10-26 | Annovis, Inc. | Magnetic dna extraction kit for plants |
| CN102344920A (en) * | 2011-10-17 | 2012-02-08 | 西安金磁纳米生物技术有限公司 | Method for purifying genomic deoxyribonucleic acid (DNA) of plant seeds by using goldmag particles |
| CN102344921A (en) * | 2011-10-17 | 2012-02-08 | 西安金磁纳米生物技术有限公司 | Method for purifying genomic deoxyribonucleic acid (DNA) of plant tissues by using goldmag particles |
-
2012
- 2012-05-02 CN CN2012101313235A patent/CN102618532A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000063362A1 (en) * | 1999-04-21 | 2000-10-26 | Annovis, Inc. | Magnetic dna extraction kit for plants |
| CN102344920A (en) * | 2011-10-17 | 2012-02-08 | 西安金磁纳米生物技术有限公司 | Method for purifying genomic deoxyribonucleic acid (DNA) of plant seeds by using goldmag particles |
| CN102344921A (en) * | 2011-10-17 | 2012-02-08 | 西安金磁纳米生物技术有限公司 | Method for purifying genomic deoxyribonucleic acid (DNA) of plant tissues by using goldmag particles |
Non-Patent Citations (6)
| Title |
|---|
| 丛玉隆: "《临床实验室管理》", 31 August 2004, 中国医药科技出版社 * |
| 何春燕等: "《医学生物化学实验指导》", 30 September 2010, 湖北科学技术出版社 * |
| 刘松财: "《生物化学实验技术》", 30 June 2010, 吉林大学出版社 * |
| 史仁玖等: "药用植物泰山四叶参DNA提取方法的研究", 《时珍国医国药》 * |
| 许良中: "《实用肿瘤病理方法学》", 31 December 1997, 上海医科大学出版社 * |
| 闫桂琴: "《遗传学实验教程》", 31 December 2010, 科学出版社 * |
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103898092B (en) * | 2014-01-27 | 2016-08-10 | 西安天隆科技有限公司 | A kind of quick paramagnetic particle method extracts test kit and the method for plant tissue genomic DNA |
| CN103898092A (en) * | 2014-01-27 | 2014-07-02 | 西安天隆科技有限公司 | Kit and method for extracting plant tissue genome DNA by adopting quick paramagnetic particle method |
| CN104630208A (en) * | 2015-02-11 | 2015-05-20 | 杭州百迈生物技术有限公司 | Kit and method for extracting genome DNA (deoxyribonucleic acid) |
| CN104630208B (en) * | 2015-02-11 | 2018-01-02 | 杭州百迈生物股份有限公司 | Extract the kit and extracting method of genomic DNA |
| CN108048449A (en) * | 2015-02-11 | 2018-05-18 | 杭州百迈生物股份有限公司 | A kind of kit for extracting genomic DNA |
| CN108048449B (en) * | 2015-02-11 | 2021-10-08 | 杭州百迈生物股份有限公司 | Kit for extracting genome DNA |
| CN106480014B (en) * | 2015-09-02 | 2020-05-15 | 创想生物科技有限公司 | Method for selectively isolating nucleic acids and kit therefor |
| CN106480014A (en) * | 2015-09-02 | 2017-03-08 | 创想生物科技有限公司 | Method for selectively isolating nucleic acids and kit therefor |
| CN106119394A (en) * | 2016-08-26 | 2016-11-16 | 刘明涛 | The nucleotide sequence authentication method of a kind of generation latitude lettuce tongue and the application of nucleotide sequence |
| CN107267502A (en) * | 2017-08-10 | 2017-10-20 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A kind of high efficiency extraction sugarcane sugar prod DNA kit and method |
| CN109055363A (en) * | 2018-09-21 | 2018-12-21 | 武汉菲沙基因信息有限公司 | A kind of magnetic bead reagent and screening technique suitable for screening overlength nucleic acids |
| CN110358762A (en) * | 2019-07-26 | 2019-10-22 | 云南省农业科学院热区生态农业研究所 | A kind of kit and its extracting method extracting plant leaf blade genomic DNA |
| CN111197073A (en) * | 2019-12-19 | 2020-05-26 | 武汉艾米森生命科技有限公司 | Method for extracting DNA sample from excrement and methylation detection method of colorectal cancer related gene |
| CN114426967A (en) * | 2020-10-29 | 2022-05-03 | 西南大学 | Non-toxic plant universal high-purity genome DNA rapid extraction method and kit |
| CN112501155A (en) * | 2020-11-06 | 2021-03-16 | 南方医科大学顺德医院(佛山市顺德区第一人民医院) | DNA extraction kit applied to transgenic mouse gene identification |
| CN114015681A (en) * | 2021-11-15 | 2022-02-08 | 深圳市人民医院 | Improved extraction method of polysaccharide polyphenol plant CTAB |
| CN115873848A (en) * | 2022-12-24 | 2023-03-31 | 蓝景科信河北生物科技有限公司 | Plant DNA extraction kit |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102618532A (en) | Kit for extracting genome DNA (Deoxyribose Nucleic Acid) from plant leaves based on paramagnetic particle method and extracting method thereof | |
| CN110684764B (en) | Lysis solution for nucleic acid extraction, nucleic acid extraction kit and nucleic acid extraction method | |
| CN109385418B (en) | Method and reagent for extracting virus/bacterium nucleic acid in animal sample | |
| CN104178480B (en) | Using the kit and method of DNA adsorption column rapid extraction DNA of plants | |
| CN101812444A (en) | Blood genome magnetic bead small-amount extraction reagent kit and extraction method thereof | |
| CN102286460B (en) | Extraction of DNA in Pleurotus eryngii by improved CTAB (cetyltrimethylammonium bromide) method | |
| CN103361339A (en) | Method for extracting filamentous fungi genome DNA and rapid screening method for kit and genetic transformant | |
| CN103898092A (en) | Kit and method for extracting plant tissue genome DNA by adopting quick paramagnetic particle method | |
| CN102199594A (en) | Method for extracting and purifying semen DNA | |
| CN107904229A (en) | Genome DNA extracting method and extracts kit | |
| CN113293159A (en) | Nucleic acid extraction kit and nucleic acid extraction method | |
| CN104404030B (en) | A kind of kit and method of rapid extraction plant genome DNA | |
| CN102161988A (en) | Method for simply, conveniently and quickly extracting trace total deoxyribonucleic acid (DNA) of single roes and fries | |
| CN103820434A (en) | Method for extracting total DNA (deoxyribonucleic acid) of fungal hyphae | |
| CN102102098A (en) | Improved phenol-chloroform method for extracting DNAs of fungi | |
| CN101735297B (en) | Method for quickly extracting low molecular weight RNA of plant | |
| CN105255857A (en) | Camellia sinensis DNA extraction method | |
| CN108624588A (en) | A method of for extracting agalloch eaglewood high quality DNA | |
| CN102703430B (en) | Method for extracting microorganism total DNA (Deoxyribonucleic Acid) in pu'er tea piling fermentation process | |
| CN101705225B (en) | High-efficiency separation method for lower molecular multiplex PCR-amplified fragments | |
| CN106636075A (en) | Method for extracting genome DNA of dendrobium officinale | |
| CN107267498A (en) | DNA kit and method is extracted in a kind of universal material from trace plant | |
| CN101125874A (en) | Method for purifying small fragment DNA in gel by grinding method and use thereof | |
| CN102888396B (en) | Method for separating low-molecular weight ribonucleic acid (RNA) of plant | |
| CN102229927A (en) | Method and reagent for improving rate of extraction of DNAs of castoff cells in case trace sample |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: SUZHOU QBIOSOURCE BIOLOGICAL TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: YI CHUN Effective date: 20140321 Free format text: FORMER OWNER: JI YUN LI KAI LI ZONGFEI Effective date: 20140321 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 201100 MINHANG, SHANGHAI TO: 215000 SUZHOU, JIANGSU PROVINCE |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20140321 Address after: Xinghu Street Industrial Park of Suzhou city in Jiangsu province 215000 BioBAY No. 218 building A2 Room 309 Applicant after: Suzhou pioneer Biotechnology Co., Ltd. Address before: 201100, 332, clear water road, Shanghai, Minhang District Applicant before: Yi Chun Applicant before: Ji Yun Applicant before: Li Kai Applicant before: Li Zongfei |
|
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120801 |