CN102617591A - Method for producing artemisinin from artemisia annua serving as Chinese herbal medicine - Google Patents
Method for producing artemisinin from artemisia annua serving as Chinese herbal medicine Download PDFInfo
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- CN102617591A CN102617591A CN2012100594763A CN201210059476A CN102617591A CN 102617591 A CN102617591 A CN 102617591A CN 2012100594763 A CN2012100594763 A CN 2012100594763A CN 201210059476 A CN201210059476 A CN 201210059476A CN 102617591 A CN102617591 A CN 102617591A
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- artemisinin
- herbal medicine
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- chinese herbal
- silica gel
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a method for producing artemisinin from artemisia annua serving as a Chinese herbal medicine. The method comprises the following steps of: crushing, percolating under high pressure, sampling continuously, performing adsorption separation on thin-layer silica gel and crystallizing and purifying 95 percent ethanol. The method has a simple process, is low in cost, high in efficiency and environment-friendly, and is suitable for the industrial production of the artemisinin. An extraction rate can be improved by the operation of percolating under the high pressure; steps and the using amount of petroleum ether are reduced by the operation of sampling continuously; and the adsorption capacity and production capacity of the artemisinin are improved by a maximum sampling method for the thin-layer silica gel.
Description
Technical field
Patent of the present invention relates to the preparation method of Artemisinin from the plant Artemisia annua herb, belongs to the extraction separation purifying field of natural product, relates to the high pressure diacolation and extracts, goes up continuously on appearance, thin-layer silicon plastic column chromatography, the maximum correlation techniques such as kind method.
Background technology
A kind of terpene monomer in the sweet wormwood prime system Chinese herbal medicine yellow flower wormwood artemisia has good result of treatment to pernicious malaria.Its traditional technology is carried for sherwood oil heat, filters, and appearance 100-200 order silica gel on the filtered liq is used 5% acetone sherwood oil mixed solvent wash-out at last then, obtains the Artemisinin of bullion.
Summary of the invention
The present invention relates to a kind of technology of from Herba Artemisiae annuae, extracting Artemisinin, comprise that the high pressure diacolation extracts, goes up appearance, thin layer silica gel absorption and purification continuously, technical process is simple, is fit to the suitability for industrialized production Artemisinin.
A kind of technology of from Herba Artemisiae annuae, extracting Artemisinin, its preparation method may further comprise the steps:
1. extract and last appearance: with raw material pulverizing to 50 order, filling is advanced in the high pressure extract post, compresses, and behind extraction column, connects a thin layer silicagel column then, in column system, leads to sherwood oil, and the Artemisinin in the medicinal powder is taken to silicagel column by sherwood oil and by the thin layer silica gel adsorption.
2. separate: treat that appearance is saturated on the silicagel column, use the sherwood oil wash-out earlier, use 5% acetone sherwood oil mixed solvent wash-out again, reclaim solvent and obtain the Artemisinin coarse-grain
3. purifying: with 95% ethanol thermosol, crystallisation by cooling obtains the pure article of Artemisinin with coarse-grain
Embodiment
1. extraction and last appearance: the Herba Artemisiae annuae herb is crushed to 50 orders, and filling is advanced in the 70ml high pressure extract post, every medicinal powder weight 40 grams, and many of palpus fillings are subsequent use.Connect 70ml thin layer silicagel column behind the extraction column, in column system, lead to 500 milliliters of sherwood oils, flow velocity 10ml/min; Artemisinin in the medicinal powder is taken to silicagel column by sherwood oil and by the thin layer silica gel adsorption, sherwood oil circulation extraction, the extraction column that renewed in 200 minutes; The point plate detects, and treats that Artemisinin absorption is saturated, stops to go up appearance; Go up 7 pillars of appearance altogether, medicinal powder amounts to 280 grams
2. separate: in the silicagel column Artemisinin absorption saturated, earlier with 200 milliliters of sherwood oil wash-outs, use 200 milliliter of 5% acetone sherwood oil mixed solvent wash-out again, reclaim mixed solvent and obtain Artemisinin coarse-grain 3.7 and restrain
3. purifying: with 100 milliliter of 95% ethanol thermosol, crystallisation by cooling obtains pure article 2.2 grams of Artemisinin with coarse-grain.
Claims (1)
1. a method of from the herbal medicine Herba Artemisiae annuae, producing Artemisinin is characterized in that, comprises the steps: that (1) insert Herba Artemisiae annuae medicinal powder in the high-pressure tower, with sherwood oil high pressure diacolation; (2) go up quadrat method continuously, appearance is carried out simultaneously in the extraction; (3) use thin layer silica gel to improve the adsorptive capacity of Artemisinin; (4) with the maximum appearance method that goes up, can reach and produce a large amount of Artemisinins.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100594763A CN102617591A (en) | 2012-03-07 | 2012-03-07 | Method for producing artemisinin from artemisia annua serving as Chinese herbal medicine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100594763A CN102617591A (en) | 2012-03-07 | 2012-03-07 | Method for producing artemisinin from artemisia annua serving as Chinese herbal medicine |
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| Publication Number | Publication Date |
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| CN102617591A true CN102617591A (en) | 2012-08-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN2012100594763A Pending CN102617591A (en) | 2012-03-07 | 2012-03-07 | Method for producing artemisinin from artemisia annua serving as Chinese herbal medicine |
Country Status (1)
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| CN (1) | CN102617591A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103054924A (en) * | 2012-12-29 | 2013-04-24 | 黄家芬 | Extraction method of artemisia annua extract |
| CN103333178A (en) * | 2013-07-29 | 2013-10-02 | 云南师范大学 | Method for preparing antimalarial active compound artemisinin through direct column chromatography |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4952603A (en) * | 1988-06-20 | 1990-08-28 | Elferaly Farouk S | Method for the isolation of artemisinin from Artemisia annua |
| CN1047503A (en) * | 1989-05-25 | 1990-12-05 | 山东省中医药研究所 | The extracting method of Artemisinin and Artemisitene |
| US6180105B1 (en) * | 1996-09-28 | 2001-01-30 | Essential Nutrition Ltd. | Method for producing an extract containing artemesinin |
| CN1869037A (en) * | 2005-05-25 | 2006-11-29 | 芜湖杉杉生物技术有限公司 | Method of extracting qinghaosu |
| CN101130548A (en) * | 2006-08-25 | 2008-02-27 | 上海诺德生物实业有限公司 | Method for extracting and producing high content arteannuin |
| CN101205232A (en) * | 2006-12-19 | 2008-06-25 | 浙江大学宁波理工学院 | Technical new method for extracting artemisinin from sweet wormwood plants by ultrasonic assistance |
-
2012
- 2012-03-07 CN CN2012100594763A patent/CN102617591A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4952603A (en) * | 1988-06-20 | 1990-08-28 | Elferaly Farouk S | Method for the isolation of artemisinin from Artemisia annua |
| CN1047503A (en) * | 1989-05-25 | 1990-12-05 | 山东省中医药研究所 | The extracting method of Artemisinin and Artemisitene |
| US6180105B1 (en) * | 1996-09-28 | 2001-01-30 | Essential Nutrition Ltd. | Method for producing an extract containing artemesinin |
| CN1869037A (en) * | 2005-05-25 | 2006-11-29 | 芜湖杉杉生物技术有限公司 | Method of extracting qinghaosu |
| CN101130548A (en) * | 2006-08-25 | 2008-02-27 | 上海诺德生物实业有限公司 | Method for extracting and producing high content arteannuin |
| CN101205232A (en) * | 2006-12-19 | 2008-06-25 | 浙江大学宁波理工学院 | Technical new method for extracting artemisinin from sweet wormwood plants by ultrasonic assistance |
Non-Patent Citations (3)
| Title |
|---|
| ALEXEI LAPKIN: "Comparative Assessment of Technologies for Extraction of Artemisinin", 《JOURNAL OF NATURAL PRODUCTS》, vol. 69, no. 11, 31 August 2006 (2006-08-31), pages 1653 - 1664 * |
| 何玉娟: "黄花蒿植物中青蒿素的提取分离过程研究", 《天津大学硕士学位论文》, 16 June 2006 (2006-06-16) * |
| 李子颖，等: "青篙素提取技术研究进展", 《中药研究与信息》, vol. 4, no. 2, 28 February 2002 (2002-02-28), pages 17 - 21 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103054924A (en) * | 2012-12-29 | 2013-04-24 | 黄家芬 | Extraction method of artemisia annua extract |
| CN103333178A (en) * | 2013-07-29 | 2013-10-02 | 云南师范大学 | Method for preparing antimalarial active compound artemisinin through direct column chromatography |
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Application publication date: 20120801 |