CN102607888A - Automatic collecting and real-time monitoring device for schistosoma japonicum cercaria - Google Patents

Automatic collecting and real-time monitoring device for schistosoma japonicum cercaria Download PDF

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CN102607888A
CN102607888A CN2011100221763A CN201110022176A CN102607888A CN 102607888 A CN102607888 A CN 102607888A CN 2011100221763 A CN2011100221763 A CN 2011100221763A CN 201110022176 A CN201110022176 A CN 201110022176A CN 102607888 A CN102607888 A CN 102607888A
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water
pond
cercaria
blood fluke
observation
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CN102607888B (en
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谢应茂
王凤鹏
曾祥志
范小林
李勋
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Gannan Normal University
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GAN NAN NORMAL COLLEGE
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Abstract

The invention relates to an automatic collecting and real-time monitoring device for schistosoma cercaria, which is formed by a water carrier, a water suction pump, an enrichment pool, an observation pool and a digital microscopy, and has the working principles that: on the basis of the water surface life habit of the schistosoma cercaria and depending on the drive action of water flow, the schistosoma cercaria is gradually conveyed and enriched into an effective observation range of the digital microscopy in the observation pool, the schistosoma cercaria entering the observation pool can be on-line observed instantly through the digital microscopy, and the states of the schistosoma cercaria can be recorded. The automatic collecting and real-time monitoring device for the schistosoma cercaria can be used for monitoring and surveying schistosoma epidemics.

Description

A kind of automatic collection and real-time monitoring device of blood fluke cercaria
Technical field
The invention belongs to the prevention and control field of snail fever, automatic enrichment that relates to a kind of blood fluke cercaria and monitoring in real time can be carried out automatic enrichment and monitor in real time the blood fluke cercaria on the large tracts of land water surface.
Background technology
Blood fluke cercaria is invaded human body skin and mucous membrane is to make the people infect the snail fever unique channel.Therefore, monitoring and kill blood fluke cercaria is one of key link in the prevention and cure of schistosomiasis work.Because the size of blood fluke cercaria has only 280~360 μ m * 60~95 μ m, can not observe directly, and takes whistle mouse and C-6 film, nylon net bag manual work to fish for the method for observing with microscope more usually at present.These method sense cycle are long, need the labor manpower and materials, and can not in time find epidemic situation.
Summary of the invention
The present invention provides a kind of cercaria with (lake, pond) on the large tracts of land water surface to collect microscopically automatically to carry out real-time device for monitoring, and this device is made up of 5 parts such as water intake, self-priming water pump, enrichment pond, observation pond and digital microscopes.
The technical solution adopted for the present invention to solve the technical problems is:
1, water intake is an infundibulate, and its upper shed is the circle of diameter 180mm, and edge of opening is smooth.50~80 mesh filter screens are equipped with in water intake upper shed place, and water intake under shed diameter is 10mm.
2, self-priming water pump adopts the 12V DC power supply, rated power 25W, and maximum flow 3L/min, the maximum journey 2m that inhales, more than the lift 10m, self-priming water pump waterpipe jointing diameter is 10mm.
3, the enrichment pond is a hydrostatic column, diameter 200mm, and high 250mm partly is made up of internal layer, skin, annular spilling water groove, water inlet pipe, rising pipe etc.Internal layer exceeds 1cm with the next door of outer interlayer than outer edge, and has the phase port in the bottom, and the 200-300 mesh filter screen is housed on the phase port.Water inlet pipe diameter 10mm passes skin to internal layer from the enrichment pond, and upward opening.Rising pipe passes the outer oblique downcomer outside to the enrichment pond from internal layer, upper shed diameter 20mm, and the upper shed edge flushes with outer brim height, under shed diameter 10mm.Annular spilling water groove has freeing port.
4, observation pond internal layer, skin and annular spilling water groove etc. are partly formed.The internal layer bottom is a upside down funnel shape, and the bottom maximum gauge is 180mm, and it is 10mm that diameter is the most carefully located at the middle part, the most carefully the place apart from observation the pond at the bottom of 200mm, top is the upper hopper mouth of diameter 80mm, upper hopper mouth edge apart from observation the pond at the bottom of 220mm.Outer diameter 200mm, high 200mm.Observation pond internal layer communicates in the bottom with outer, and the 200-300 mesh filter screen is housed, and outer is annular spilling water groove on every side, and the spilling water groove has the draining mouth of pipe.Bottom, observation pond raises up, and height 100mm is equipped with the LED illuminating lamp.
5, digital micro-analysis camera (containing led light source) is installed in directly over the internal layer of observation pond.
The coboundary that makes water intake during erecting device uses diameter to connect as the 10mm water pipe water intake, self-priming pump, enrichment pond water inlet pipe a little less than the lake water water surface.According to the habit of cercaria in the water surface life, cercaria will flow in the water intake with superficial water, deliver in the internal layer of enrichment pond through self-priming water pump.The water surface life habit of the effect of filter screen and cercaria will make cercaria stay internal layer and concentrate on the surface, and after the water in the enrichment pond is filled, the water that major part does not contain cercaria will spill into the annular spilling water groove from skin, flow back in the lake by freeing port again.Because rising pipe upper shed edge and outer brim height are on the same surface level, and inside and outside two-layer bottom communicate, so the internal layer surface water will carry cercaria and flow out through rising pipe.The water that flows out through rising pipe with the ratio of the water of enrichment pond freeing port discharge is:
Figure BSA00000422401900021
only can flow out from rising pipe because of cercaria, thereby can reach the effect of enrichment.
The water delivering orifice of enrichment pond rising pipe places upper hopper mouth top, observation pond.The cercaria of from the enrichment pond, flowing out is slipped into observation pond internal layer along observation pond upper hopper oral-lateral wall, owing to have filter screen and cercaria to have the water surface life habit, cercaria will concentrate on to be observed on the water surface that the pond internal layer the most carefully locates, and water is got back in the lake through annular spilling water groove and drainpipe.Under the illumination of LED lamp, observe, monitor, record a video and take pictures by digital microscope.
Utilize the phototropism of blood fluke cercaria, the top led light source is for often opening, and the led light source of bottom is only opened when needs are taken the record clear pictures.For avoiding blood fluke cercaria to be bonded on the device inwall, device adopts lubricious material (like glass from, stainless steel etc.) to make, and can embrocate the agent of one deck expelling water at device inwall (particularly water surface place).
The invention has the beneficial effects as follows, can realize that whether water field of big area such as lake surface, pond are contained blood fluke cercaria is carried out real-time automatic monitoring, in time find the blood fluke epidemic situation, save a large amount of manpower and materials, improve prevention and cure of schistosomiasis work.
Description of drawings
Accompanying drawing is a structural representation of the present invention.
1. water intakes among the figure, 2.50~80 mesh filter screens, 3. self-priming water pump, 4. enrichment pond water inlet pipe; 5. enrichment pond internal layer, 6. the enrichment pond is outer, 7. enrichment pond rising pipe, 8.200~300 mesh filter screens; 9. annular spilling water groove, 10. observation pond internal layer, 11. observation ponds are outer, 12. observation Chi Shangloudoukou; 13. the bottom led light source, 14. digital micro-analysis cameras (containing led light source), 15. water pipes, 16. freeing port.
Embodiment
Below in conjunction with accompanying drawing and embodiment the present invention is done further explain.
Embodiment 1
1, the coboundary that requires water intake (1) during erecting device is a little less than the lake water water surface, and 50~80 mesh filter screens (2) can let the blood blood fluke cercaria get into and prevent that the large scale impurity on the lake surface from getting into.
2, water pipe (15) links to each other the under shed of water intake (1) with the inlet port of self-priming water pump (3), and the water delivering orifice of self-priming water pump (3) links to each other with enrichment pond water inlet pipe (4).
3, according to the habit of blood fluke cercaria in water surface life, blood fluke cercaria will flow in the water intake (1) with superficial water, deliver in the internal layer of enrichment pond in (5) through self-priming water pump (3).According to law of connected vessels, (5) are the same with the water surface elevation of enrichment pond outer (6) in the internal layer of enrichment pond.Because the effect of filter screen (8) and the water surface life habit of blood fluke cercaria will make blood fluke cercaria only can stay internal layer and concentrate on the surface; After the water in the enrichment pond is filled; The water that major part does not contain blood fluke cercaria will spill in the annular spilling water groove (9), flow back in the lake through freeing port (16) again.The blood fluke cercaria density on internal layer surface constantly increases, and will be with rising pipe (7) outflow through the enrichment pond of few part water.
4, the water delivering orifice of enrichment pond rising pipe (7) places observation Chi Shangloudoukou (12) top.The blood fluke cercaria that from the enrichment pond, flows out will slip into observation pond internal layer (10) along the sidewall of observation Chi Shangloudoukou (12).Owing to have the effect of filter screen (8) and blood fluke cercaria to have the water surface life habit, blood fluke cercaria will concentrate on to be observed on the internal layer water surface of pond.The water that does not contain blood fluke cercaria spills in the annular spilling water groove (9), gets back in the lake through freeing port (16) again.Because the height the most carefully locate of observation pond internal layer (10) is identical with the height of observing pond skin (11), so observes the water surface position of pond internal layer (10) just be in and the most carefully locate, thereby can improve bilharzial density.
5, digital micro-analysis camera (14) focuses on the water surface position of observation pond internal layer (10), blood fluke cercaria is observed, is taken pictures and records a video, thereby reach the purpose of blood fluke cercaria being carried out real-time automatic monitoring.Because of blood fluke cercaria has phototropism, the light source on the digital micro-analysis camera (14) is a normally open, helps blood fluke cercaria and concentrates on digital micro-analysis camera (14) below fast, improves monitoring efficient.Because the reflecting effect of water surface is not good,, needs will open bottom led light source (13) when taking the record clear pictures again.

Claims (6)

1. the present invention relates to a kind of automatic collection and real-time monitoring device of blood fluke cercaria, it is characterized in that: this blood fluke cercaria is collected automatically and real-time monitoring device mainly is made up of device for fetching water from, suction pump, enrichment pond, observation pond and digital microscope.
2. device for fetching water from according to claim 1; It is characterized in that: the shape of device for fetching water from is flat funnel-form; After putting into water, can rely on self buoyancy to bubble through the water column automatically, and the funnel edge of opening is a little less than the water surface; Thereby the blood fluke cercaria nature is flowed in the device for fetching water from, again in suction pump is sent to the enrichment pond with superficial water.
3. enrichment according to claim 1 pond is characterized in that: the enrichment pond is divided inside and outside two-layer, and internal layer communicates in the bottom with outer interlayer; Utilize the water surface life habit of blood fluke cercaria; Completely from the mode of overflowing, make the inflow in enrichment pond and aquifer yield reach self-poise through water, blood fluke cercaria is flow to the observation pond from rising pipe; Automatically the water that overflows does not then contain blood fluke cercaria, thereby reaches the purpose of automatic enrichment.
4. observation according to claim 1 pond; It is characterized in that: observation pond internal layer is the upside down funnel shape, and docks with digital microscope, and Chi Zhongshui is completely from overflowing; Make inflow and aquifer yield self-poise; It is stable to keep water surface site, and blood fluke cercaria then slips into observation pond internal layer from sidewall, is in the internal layer surface layer of water to guarantee blood fluke cercaria.
5. the automatic collection of blood fluke cercaria according to claim 1; It is characterized in that: based on the water surface life habit of blood fluke cercaria; Rely on the induced effect of current, with blood fluke cercaria progressively transmit, in effective observation scope of enrichment digital microscope in the observation pond.
6. the real-time monitoring of blood fluke cercaria according to claim 1 is characterized in that: through digital microscope the blood fluke cercaria that gets in the observation pond is carried out online, immediate observation, and its state is carried out record.
CN201110022176.3A 2011-01-20 2011-01-20 A kind of automatic collection of blood fluke cercaria and real-time monitoring device Expired - Fee Related CN102607888B (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103674954A (en) * 2013-12-13 2014-03-26 江苏省血吸虫病防治研究所 Device and method for detecting schistosoma japonicum katsurada infectious water body
CN104819969A (en) * 2015-05-18 2015-08-05 赣南师范学院 Method for preparing up-conversion fluorescence indicator of schistosome cercaria rare earth
CN105699379A (en) * 2016-03-14 2016-06-22 南京市疾病预防控制中心 Schistosoma myracidium detection device
CN106093036A (en) * 2016-06-02 2016-11-09 赣南师范学院 A kind of rotary schistosomulum enrichment and imaging device
CN106404457A (en) * 2016-10-28 2017-02-15 长沙理工大学 Sampling device for worms in drinking water and use method of sampling device

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US7501095B2 (en) * 2003-07-04 2009-03-10 National Taipei University Of Technology Apparatus for on-line sampling of metal nanoparticle fluid and technique of the same
CN201072408Y (en) * 2007-06-18 2008-06-11 中国农业科学院上海兽医研究所 Schistosome miracidium electronic display device
CN201233332Y (en) * 2008-07-11 2009-05-06 湖南省血吸虫病防治所 Cow dropping quantitative determination sampler

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103674954A (en) * 2013-12-13 2014-03-26 江苏省血吸虫病防治研究所 Device and method for detecting schistosoma japonicum katsurada infectious water body
CN104819969A (en) * 2015-05-18 2015-08-05 赣南师范学院 Method for preparing up-conversion fluorescence indicator of schistosome cercaria rare earth
CN105699379A (en) * 2016-03-14 2016-06-22 南京市疾病预防控制中心 Schistosoma myracidium detection device
CN105699379B (en) * 2016-03-14 2018-07-31 南京市疾病预防控制中心 Schistosoma miracidium detection device
CN106093036A (en) * 2016-06-02 2016-11-09 赣南师范学院 A kind of rotary schistosomulum enrichment and imaging device
CN106093036B (en) * 2016-06-02 2018-10-16 赣南师范大学 A kind of rotary blood fluke cercaria enrichment and imaging device
CN106404457A (en) * 2016-10-28 2017-02-15 长沙理工大学 Sampling device for worms in drinking water and use method of sampling device
CN106404457B (en) * 2016-10-28 2018-09-11 长沙理工大学 Worm sampler and its application method in a kind of drinking water

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Address after: 341000 Ganzhou Economic Development Zone, Ganzhou, Jiangxi

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