CN102604908B - Lipase mutant with high catalytic activity - Google Patents
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Abstract
The invention relates to lipase mutant with high catalytic activity. A preparation method for preparing the lipase mutant comprises the following steps: performing recombination and site directed mutation for multiple times to Rhizopuschinensis CCTCC (China Center For Type Culture Collection) M201021 lipase gene through an error prone PCR (Polymerase Chain Reaction) method, and carrying out directed evolution to obtain the lipase mutant, wherein a Genbank accession number of Rhizopuschinensis CCTCC M201021 lipase gene is EF405962; amino acid mutant Ser373Asn is contained in amino acid sequence of the mutant; and enzyme activity of the lipase mutant is enhanced in comparison with that of an original strain when a turnover number Kcat is used to express enzyme activity.
Description
The application is dividing an application of original applying number 200910235185.3, applying date 2009.11.11, denomination of invention " lipase mutant that the vigor that makes up by orthogenesis improves ".
Technical field
The invention belongs to the gene engineering technology field of enzyme, relate in particular to the zhizopchin lipase mutant that enzyme activity improves.
Background technology
Lipase (EC 3.1.1.3) can not only catalyzing oil hydrolysis by using, also can be in nonaqueous phase the reactions such as synthetic, the transesterification of catalysis ester, acidolysis, be widely used in chemistry, food is in pharmacy and washing composition or the bioenergy industry.Microorganism is an important sources of lipase, and head mold is the important production bacterium of microbial lipase.Nowadays, existingly surpass 30 kinds of rizolipases and realized commercialization production.Rizolipase mostly has height 1, and therefore the 3-regioselectivity is usually used in the grease processing.In addition, rizolipase also has good stability, and the transformation efficiency advantages of higher is widely used in the production of aromatic ester, biofuel, chipal compounds.
So far, reported the gene order of a plurality of rizolipases both at home and abroad.Japan, Germany to Rhizopus oryzae lipase (
Rhizopus oryzaeLipase,
ROL) gene order and express and to have done more deep research, and successively with intestinal bacteria, yeast saccharomyces cerevisiae and pichia pastoris phaff successful expression lipase gene (Minning S et al.
J Biotechnol, 1998,66: 147-156; Beer HD et al.
Biochim Biophys Acta, 1998,1399: 173-180; Ueda M et al.
J Mol Catal B:Enzym, 2002,17: 113-124).The contriver in early-stage Study, successfully from the distiller's yeast of brewing aroma type yeast wine, screen a plant height yielding lipase zhizopchin (
Rhizopus chinensisCCTCC M 201021) bacterial strain, and from this bacterial strain clone obtain lipase gene sequence (Genbank accession number EF405962), and realize this lipase pichia pastoris phaff (
Pichia pastoris) in high-level secretory expression (Yu Xiao-Wei et al.
J Mol Catal B:Enzym, 2009,57:304-311).
Orthogenesis belongs to irrational design, refer to by simulation Darwin natural evolution process in the laboratory, for the gene of a certain protease, the gene of the enzyme of transforming by improved induced-mutation technique, then according to the specific purpose of transforming, screen valuable natural enzyme.Over nearly 10 years, the orthogenesis technology obtains huge success in esterase and lipase character transformation field, mainly concentrates on the catalytic reaction activity that improves enzyme, improves substrate specificity, improve thermostability, the aspects such as enantio-selectivity (Johannes TW et al.
Curr. Opin. Microbiol, 2006,9:261-267).
The raising of enzyme activity can be used the kinetic parameter of enzyme
K CatValue represents.
K CatClaim again turn over number, refer to the substrate molecule number (TN) of per molecule enzyme or each enzyme active center energy catalysis within the unit time, be also referred to as catalytic constant.Therefore,
K CatThe raising of value has namely represented the raising that enzyme is lived.
Summary of the invention
The technical problem that the present invention solves provides the zhizopchin lipase that enzyme activity improves.
Technical scheme of the present invention: the lipase mutant that the vigor that makes up by orthogenesis improves, by zhizopchin (
Rhizopus chinensis) CCTCC M 201021 lipase genes (Genbank accession number EF405962), use the fallibility PCR method, by many wheel restructuring and rite-directed mutagenesis, the lipase mutant that obtains through orthogenesis, in the aminoacid sequence of mutant, comprise the combination of amino acid mutation Ala129Ser, Lys161Arg, Thr195Tyr, Ala230Thr, Val261Gly, Lys322Arg, Ser373Asn and two, three or four sudden changes of above-mentioned amino acid; With turnover number
K CatExpression, the enzyme work of lipase mutant is improved than starting strain; The sequencing result of mutant and
K CatThe multiple that improves is:
| Lipase mutant | The aminoacid replacement position | K cat(/min) | K catThe multiple that improves |
| Starting strain lipase | - | 1138 | 1.0 |
| Mutant 1-1 | Ala129Ser | 1252 | 1.1 |
| Mutant 1-2 | Lys161Arg | 1479 | 1.3 |
| Mutant 1-3 | Thr195Tyr | 1479 | 1.3 |
| Mutant 1-4 | Ala230Thr | 1821 | 1.6 |
| Mutant 1-5 | Val261Gly | 1935 | 1.7 |
| Mutant 1-6 | Lys322Arg | 1366 | 1.2 |
| Mutant 1-7 | Ser373Asn | 1707 | 1.5 |
| Mutant 2-1 | Ala129Ser / Lys161Arg | 2278 | 2 |
| Mutant 2-2 | Ala129Ser / Ala230Thr | 1365 | 1.2 |
| Mutant 2-3 | Ala129Ser / Val261Gly | 1593 | 1.4 |
| Mutant 2-4 | Lys161Arg/Thr195Tyr | 2845 | 2.5 |
| Mutant 2-5 | Lys161Arg / Lys322Arg | 1707 | 1.5 |
| Mutant 2-6 | Lys161Arg / Ser373Asn | 2048 | 1.8 |
| Mutant 2-7 | Ala230Thr /Ser373Asn | 1707 | 1.5 |
| Mutant 2-8 | Val261Gly/ Ser373Asn | 3726 | 3.3 |
| Mutant 3-1 | Ala129Ser/Ala230Thr /Val261Gly | 3186 | 2.8 |
| Mutant 3-2 | Ala129Ser/Ala230Thr/Lys322Arg | 2278 | 2 |
| Mutant 3-3 | Lys161Arg/Thr195Tyr/Ser373Asn | 2617 | 2.3 |
| Mutant 3-4 | Lys161Arg/Ala230Thr/Ser373Asn | 2278 | 2 |
| Mutant 3-5 | Lys161Arg/Val261Gly/Lys322Arg | 2731 | 2.4 |
| Mutant 3-6 | Ala230Thr/Lys322Arg/Ser373Asn | 2845 | 2.5 |
| Mutant 4-1 | Ala129Ser/Lys161Arg/Ala230Thr/ Lys322Arg | 2073 | 1.8 |
Zhizopchin lipase amino acid original series is: SEQ ID NO:1; Zhizopchin lipase amino acid mutation sequence is: SEQ ID NO:2,7 mutating acids show with the background color mark.
Beneficial effect of the present invention: the present invention uses fallibility PCR method rizolipase gene to China (Genbank accession number EF405962) to carry out orthogenesis, by many wheel restructuring and rite-directed mutagenesis, obtain the zhizopchin lipase mutant, it is prominent that these mutant comprise amino acid mutation Ala129Ser, Lys161Arg, Thr195Tyr, Ala230Thr, Val261Gly, Lys322Arg, Ser373Asn and above-mentioned amino acid
The combination that becomes.With turnover number
K CatExpression, the enzyme work of lipase mutant is improved.
Embodiment
The culture medium prescription that relates among the embodiment is as follows:
The LB liquid nutrient medium: peptone 1%, yeast extract 0.5%, NaCl 1%, pH7.0.
YPD (Yeast Extract Peptone Dextrose Medium): Yeast Extract 1%, Trypton 2%, and Dextrose 2%, adds Agar 2% when making flat board.121 ° of C autoclaving 20 min.Adding G418 when being used for screening G418 resistance is 0.25 mg/mL-1.0 mg/mL to final concentration, and namely YPD-G418 is dull and stereotyped.
MD(Minimal Dextrose Medium): YNB 1.34％, Biotin 4×10
-5％, Dextrose 2％, Agar 2％。
MM(Minimal Methanol Medium): YNB1.34％, Biotin4×10
-5％, Methanol 0.5％, Agar2％。
BMGY (Buffered Glycerol-complex Medium): Yeast Extract 1%, Trypton 2%, and YNB 1.34%, and Biotin 4 * 10
-5%, Glycerol 1%, potassium phosphate solution 100 mmol/L.
BMMY (Buffered Methanol-complex Medium): Yeast Extract 1%, Trypton 2%, and YNB 1.34%, and Biotin 4 * 10
-5%, Methanol 0.5%, potassium phosphate solution 100 mmol/L.
Unit in the substratum is %(W/V)
Embodiment 1, utilize the fallibility PCR method to make up zhizopchin lipase sudden change library
Utilize the fallibility round pcr to introduce coding mutation external to zhizopchin lipase gene proRCL.The reaction conditions of fallibility PCR is as follows:
Wherein, primers F and R sequence are:
Upstream primer F:5'-TCAAGATCCCTAGGGTTCCTGTTGGTCATAAAGGTTC-3';
Downstream primer R:5'-AATTCCAGTGCGGCCGCTTACAAACAGCTTCCTTCG-3'.
Pcr amplification condition: 94 ℃ of 3min; 94 ℃ of 1 min, 59 ℃ of 1 min, 72 ℃ of 2 min, 30 circulations; 72 ℃ of 10 min.
The fallibility pcr amplification product behind DNA purification kit purifying, restriction enzyme
AvrII and
NotI digests fallibility pcr amplification product and plasmid pPIC9K respectively, connects, and is converted into E .coli JM109 competent cell.Coat the Amp that LB(contains 100 μ g/ μ L) flat board.Grow behind 12 h, transformant is transferred in the LB liquid nutrient medium cultivates, obtain mutant plasmid.
With mutant plasmid through restriction enzyme
SalAfter the I linearizing, electricity transforms the Pichia pastoris GS115 competent cell.Conversion fluid is coated on the MD flat board, cultivated 2 days for 30 ℃, consist of the sudden change library.
Utilize above-mentioned same method, carry out many wheel fallibility PCR take the mutant gene group as masterplate, make up the sudden change library.
The screening of embodiment 2, high enzyme lipase mutant alive
With sterilizing toothpick with the His that grows on the MD flat board
+Transformant copies to the same position of YPD and BMMY flat board, will contrast simultaneously bacterium GS115/ pPIC9K-proRCL and be seeded on the BMMY flat board.30 ° of C cultivated 2 days.
Dull and stereotyped primary dcreening operation: it is dull and stereotyped to preserve the complete YPD of growth.Per 12 h cover to the BMMY plate and add the expression of 200 μ L methanol induction recombinant lipases.Induced 2-3 days.In enzyme activity determination substrate pNPP, add 0.6% agar, after mixing, fall in the dull and stereotyped top-agar that forms of BMMY.Obviously yellow bacterial strain occurring in 2 min is primary dcreening operation purpose mutant strain.Under the same terms, contrast bacterium GS115/ pPIC9K-proRCL can not demonstrate obvious yellow.
96 orifice plates sieve again: add 300 μ L BMGY substratum in 96 orifice plates in 1.8 mL/ holes (flat), 121 ℃ of sterilization 20 min.Be preserved in primary dcreening operation purpose bacterial strain (accessing simultaneously GS115/ pPIC9K-proRCL in contrast) on the YPD flat board to access wherein, 30 ℃ of 250 r/min shaking culture to OD600 be the about 16-18 h of 2-6().Centrifugal, abandon supernatant, with the resuspended thalline of 900 μ L BMMY substratum, and add 1%(V/V) expression of methanol induction lipase.After this per 24 h add 100 μ L BMMY substratum and 1 % (V/V) methyl alcohol, induce 4 days.With centrifugal 10 min of 96 orifice plate fermented liquids, 3000 r/min of abduction delivering 96 h, collect supernatant.After getting 500 times of 1 μ L supernatant liquor dilutions, get 5 μ L in another 96 orifice plate, add substrate with the volley of rifle fire, the vibration mixing.Show rapidly in 2 min that obviously yellow bacterial strain is the bacterial strain of multiple sieve mesh.Under the same terms, the fermented supernatant fluid of contrast bacterium GS115/ pPIC9K-proRCL can not show obvious yellow.
Screen as the basis take the sudden change library that fallibility PCR makes up, obtain the 6 strain enzymes bacterial strain that obviously improves alive, measure the lipase nucleotide sequence, utilize triplet codon to infer the aminoacid sequence of lipase, the aminoacid replacement of lipase mutant reaches
K CatIt is as shown in table 1 that value improves multiple.Measure lipase mutant according to the method for embodiment 3
K CatValue.
The sequencing result of table 1 mutant
| Lipase mutant | The aminoacid replacement position | K cat(/min) | K catThe multiple that improves |
| Starting strain lipase | - | 1138 | 1.0 |
| Mutant 2-1 | Ala129Ser / Lys161Arg | 2278 | 2 |
| Mutant 2-4 | Lys161Arg/Thr195Tyr | 2845 | 2.5 |
| Mutant 2-7 | Ala230Thr /Ser373Asn | 1707 | 1.5 |
| Mutant 2-8 | Val261Gly/ Ser373Asn | 3726 | 3.3 |
| Mutant 3-2 | Ala129Ser/Ala230Thr/Lys322Arg | 2278 | 2 |
| Mutant 3-4 | Lys161Arg/Ala230Thr/Ser373Asn | 2278 | 2 |
Embodiment 3 lipase mutants
K CatPH-value determination pH
For measuring lipase
K CatValue need to be carried out separation and purification to enzyme.
Shake flask fermentation: with starting strain and each lipase mutant, be seeded in the 25 mL BMGY substratum 30 ℃ of shaking culture 16～20 h to OD
600Be 2～6, centrifugal collection thalline is diluted to OD with the BMMY substratum
600Be 1, add the methanol induction of 0.5 % every 24 h and express, cultivate after 3-4 days, collect fermented supernatant fluid.
Separation and purification: the fermented supernatant fluid of mutant strain is concentrated through 10 KD ultra-filtration membranes, obtain the sudden change lipase activity component of purifying behind SP-Sepharose FF strong cation exchange chromatography and the Phenyl-Sepharose 6 FF hydrophobic chromatography column chromatographies.Concrete operations reference Yu Xiao-Wei et al.
J Mol Catal B:Enzym, 2009,57:304-311.
Measuring method:
The measuring method of lipase activity is pNPP method (Pencreach G et al.
Enzyme and Microbial Technol.1996,18:417-422.).Enzyme is lived and to be defined as enzyme amount that per minute under certain reaction conditions produces 1 μ mol p-NP is the lipase hydrolysis enzyme international unit of living.Be in 0～25 mmol/L scope in substrate p-NP cetylate concentration, measure enzyme activity, calculate the kinetic parameter that obtains lipase
K Cat
The gene mutation site of embodiment 4 rite-directed mutagenesis combined lipase mutant
Through the too much wheel fallibility PCR library construction that suddenlys change, obtain including 7 mutant strains of amino acid mutation site Ala129Ser, Lys161Arg, Thr195Tyr, Ala230Thr, Val261Gly, Lys322Arg, Ser373Asn.Reach the combination of each sudden change to the impact of lipase mutant strain vigor in order to investigate wherein some sudden changes, the rite-directed mutagenesis combination is carried out in the mutational site of finding, obtain a plurality of lipase mutants.(rite-directed mutagenesis can utilize commercially available test kit to carry out.）
The gene that will contain the said mutation Sites Combination is connected with carrier pPIC9K, and electricity transforms Pichia pastoris GS115, to obtain the efficient secretory expression of lipase.Measure lipase mutant with the method that is equal to embodiment 3
K CatValue.The combination site of each mutant enzyme and
K CatThe multiple that improves is as shown in table 2.
The sequencing result of table 2 mutant and
K CatThe multiple that improves
| Lipase mutant | The aminoacid replacement position | K cat(/min) | K catThe multiple that improves |
| Starting strain lipase | - | 1138 | 1.0 |
| Mutant 1-1 | Ala129Ser | 1252 | 1.1 |
| Mutant 1-2 | Lys161Arg | 1479 | 1.3 |
| Mutant 1-3 | Thr195Tyr | 1479 | 1.3 |
| Mutant 1-4 | Ala230Thr | 1821 | 1.6 |
| Mutant 1-5 | Val261Gly | 1935 | 1.7 |
| Mutant 1-6 | Lys322Arg | 1366 | 1.2 |
| Mutant 1-7 | Ser373Asn | 1707 | 1.5 |
| Mutant 2-2 | Ala129Ser / Ala230Thr | 1365 | 1.2 |
| Mutant 2-3 | Ala129Ser / Val261Gly | 1593 | 1.4 |
| Mutant 2-5 | Lys161Arg / Lys322Arg | 1707 | 1.5 |
| Mutant 2-6 | Lys161Arg / Ser373Asn | 2048 | 1.8 |
| Mutant 3-1 | Ala129Ser/Ala230Thr /Val261Gly | 3186 | 2.8 |
| Mutant 3-3 | Lys161Arg/Thr195Tyr/Ser373Asn | 2617 | 2.3 |
| Mutant 3-5 | Lys161Arg/Val261Gly/Lys322Arg | 2731 | 2.4 |
| Mutant 3-6 | Ala230Thr/Lys322Arg/Ser373Asn | 2845 | 2.5 |
| Mutant 4-1 | Ala129Ser/Lys161Arg/Ala230Thr/ Lys322Arg | 2073 | 1.8 |
<210> SEQ ID NO: 1
<211> 389
<212> PRT
＜213〉zhizopchin (
Rhizopus chinensis) CCTCC M 201021 lipase amino acid
<400> 1
Met Val Ser Phe Ile Ser Ile Ser Gln Gly Val Ser Leu Cys Leu
5 10 15
Leu Val Ser Ser Met Met Leu Gly Ser Ser Ala Val Pro Val Ala
20 25 30
Gly His Lys Gly Ser Val Lys Ala Thr Asn Gly Thr Asp Phe Gln
35 40 45
Leu Pro Pro Leu Ile Ser Ser Arg Cys Thr Pro Pro Ser His Pro
50 55 60
Glu Thr Thr Gly Asp Pro Asp Ala Glu Ala Tyr Tyr Ile Asn Lys
65 70 75
Ser Val Gln Trp Tyr Gln Ala His Gly Gly Asn Tyr Thr Ala Leu
80 85 90
Ile Lys Arg Asp Thr Glu Thr Val Gly Gly Met Thr Leu Asp Leu
95 100 105
Pro Glu Asn Pro Pro Pro Ile Pro Ala Thr Ser Thr Ala Pro Ser
110 115 120
Ser Asp Ser Gly Glu Val Val Thr Ala Thr Ala Ala Gln Ile Lys
125 130 135
Glu Leu Thr Asn Tyr Ala Gly Val Ala Ala Thr Ala Tyr Cys Arg
140 145 150
Ser Val Val Pro Gly Thr Lys Trp Asp Cys Lys Gln Cys Leu Lys
155 160 165
Tyr Val Pro Asp Gly Lys Leu Ile Lys Thr Phe Thr Ser Leu Leu
170 175 180
Thr Asp Thr Asn Gly Phe Ile Leu Arg Ser Asp Ala Gln Lys Thr
185 190 195
Ile Tyr Val Thr Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile
200 205 210
Thr Asp Met Val Phe Thr Phe Thr Asp Tyr Ser Pro Val Lys Gly
215 220 225
Ala Lys Val His Ala Gly Phe Leu Ser Ser Tyr Asn Gln Val Val
230 235 240
Lys Asp Tyr Phe Pro Val Val Gln Asp Gln Leu Thr Ala Tyr Pro
245 250 255
Asp Tyr Lys Val Ile Val Thr Gly His Ser Leu Gly Gly Ala Gln
260 265 270
Ala Leu Leu Ala Gly Met Asp Leu Tyr Gln Arg Glu Lys Arg Leu
275 280 285
Ser Pro Lys Asn Leu Ser Ile Tyr Thr Val Gly Cys Pro Arg Val
290 295 300
Gly Asn Asn Ala Phe Ala Tyr Tyr Val Asp Ser Thr Gly Ile Pro
305 310 315
Phe His Arg Thr Val His Lys Arg Asp Ile Val Pro His Val Pro
320 325 330
Pro Gln Ala Phe Gly Tyr Leu His Pro Gly Val Glu Ser Trp Ile
335 340 345
Lys Glu Asp Pro Ala Asp Val Gln Ile Cys Thr Ser Asn Ile Glu
350 355 360
Thr Lys Gln Cys Ser Asn Ser Ile Val Pro Phe Thr Ser Ile Ala
365 370 375
Asp His Leu Thr Tyr Phe Gly Ile Asn Glu Gly Ser Cys Leu
380 385 389
<210> SEQ ID NO: 2
<211> 389
<212> PRT
＜213〉zhizopchin (
Rhizopus chinensis) CCTCC M 201021 lipase amino acid mutation bodies
<400> 2
Met Val Ser Phe Ile Ser Ile Ser Gln Gly Val Ser Leu Cys Leu
5 10 15
Leu Val Ser Ser Met Met Leu Gly Ser Ser Ala Val Pro Val Ala
20 25 30
Gly His Lys Gly Ser Val Lys Ala Thr Asn Gly Thr Asp Phe Gln
35 40 45
Leu Pro Pro Leu Ile Ser Ser Arg Cys Thr Pro Pro Ser His Pro
50 55 60
Glu Thr Thr Gly Asp Pro Asp Ala Glu Ala Tyr Tyr Ile Asn Lys
65 70 75
Ser Val Gln Trp Tyr Gln Ala His Gly Gly Asn Tyr Thr Ala Leu
80 85 90
Ile Lys Arg Asp Thr Glu Thr Val Gly Gly Met Thr Leu Asp Leu
95 100 105
Pro Glu Asn Pro Pro Pro Ile Pro Ala Thr Ser Thr Ala Pro Ser
110 115 120
Ser Asp Ser Gly Glu Val Val Thr Ser Thr Ala Ala Gln Ile Lys
125 130 135
Glu Leu Thr Asn Tyr Ala Gly Val Ala Ala Thr Ala Tyr Cys Arg
140 145 150
Ser Val Val Pro Gly Thr Lys Trp Asp Cys Arg Gln Cys Leu Lys
155 160 165
Tyr Val Pro Asp Gly Lys Leu Ile Lys Thr Phe Thr Ser Leu Leu
170 175 180
Thr Asp Thr Asn Gly Phe Ile Leu Arg Ser Asp Ala Gln Lys Tyr
185 190 195
Ile Tyr Val Thr Phe Arg Gly Thr Asn Ser Phe Arg Ser Ala Ile
200 205 210
Thr Asp MET Val Phe Thr Phe Thr Asp Tyr Ser Pro Val Lys Gly
215 220 225
Ala Lys Val His Thr Gly Phe Leu Ser Ser Tyr Asn Gln Val Val
230 235 240
Lys Asp Tyr Phe Pro Val Val Gln Asp Gln Leu Thr Ala Tyr Pro
245 250 255
Asp Tyr Lys Val Ile Gly Thr Gly His Ser Leu Gly Gly Ala Gln
260 265 270
Ala Leu Leu Ala Gly Met Asp Leu Tyr Gln Arg Glu Lys Arg Leu
275 280 285
Ser Pro Lys Asn Leu Ser Ile Tyr Thr Val Gly Cys Pro Arg Val
290 295 300
Gly Asn Asn Ala Phe Ala Tyr Tyr Val Asp Ser Thr Gly Ile Pro
305 310 315
Phe His Arg Thr Val His Arg Arg Asp Ile Val Pro His Val Pro
320 325 330
Pro Gln Ala Phe Gly Tyr Leu His Pro Gly Val Glu Ser Trp Ile
335 340 345
Lys Glu Asp Pro Ala Asp Val Gln Ile Cys Thr Ser Asn Ile Glu
350 355 360
Thr Lys Gln Cys Ser Asn Ser Ile Val Pro Phe Thr Asn Ile Ala
365 370 375
Asp His Leu Thr Tyr Phe Gly Ile Asn Glu Gly Ser Cys Leu
380 385 389
<210> SEQ ID NO: 3
<400> 3
F: 5'-TCAAGATCCCTAGGGTTCCTGTTGGTCATAAAGGTTC-3';
R: 5'-AATTCCAGTGCGGCCGCTTACAAACAGCTTCCTTCG-3'。
Claims (1)
1. high catalysis activity lipase mutant, it is characterized in that by zhizopchin (
Rhizopus chinensis) CCTCC M 201021 lipase genes, its Genbank accession number EF405962 uses the fallibility PCR method, by many wheel restructuring and rite-directed mutagenesis, the lipase mutant that obtains through orthogenesis in the aminoacid sequence of mutant, comprises amino acid mutation Ser373Asn; With turnover number
K CatExpression, the enzyme work of lipase mutant is improved than starting strain; The sequencing result of mutant and
K CatThe multiple that improves is:
。
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| CN102604909B (en) * | 2009-11-11 | 2013-07-17 | 江南大学 | Directed-evolution structured lipase mutant with enhanced catalytic activity |
| CN102586203B (en) * | 2009-11-11 | 2013-04-17 | 江南大学 | Determinate-evolution-constructed lipase mutant with improved catalysis activity |
| CN102978182B (en) * | 2012-12-29 | 2014-05-14 | 青岛蔚蓝生物集团有限公司 | Lipase mutant |
| WO2015091746A1 (en) | 2013-12-20 | 2015-06-25 | Novozymes A/S | Compositions and processes for treatment with lipases |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1443841A (en) * | 2003-04-22 | 2003-09-24 | 江南大学 | Lipasegenous bactenia, its screening method and industrial application |
| CN101544966A (en) * | 2009-04-28 | 2009-09-30 | 江南大学 | Zymolysis preparation method of recombinant yeast lipase |
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2009
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1443841A (en) * | 2003-04-22 | 2003-09-24 | 江南大学 | Lipasegenous bactenia, its screening method and industrial application |
| CN101544966A (en) * | 2009-04-28 | 2009-09-30 | 江南大学 | Zymolysis preparation method of recombinant yeast lipase |
Non-Patent Citations (2)
| Title |
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| 唐良华 等.微生物脂肪酶分子改造研究进展.《生物技术》.2003,第13卷(第3期),42-44. * |
| 王乐乐 等.华根霉(Rhizopus chinensis)前导肽脂肪酶基因的克隆及其在Pichia pastoris中的表达.《高技术通讯》.2009,第19卷(第1期),105-110. * |
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