CN102599643A - 一种能减轻梗丝木质气的复合料液及其应用 - Google Patents
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Abstract
本发明公开了一种能减轻梗丝木质气的复合料液及其应用,涉及烟草加工领域。将液体漆酶与香料物质按照1∶30的质量比,在4℃条件下混合均匀后制成复合料液。并采用空气压缩的方式按2.5%的质量比喷洒到已切片的梗丝上,储梗丝3h后,采用气流干燥烘丝,冷却后加香,得到成品梗丝,从而达到减轻梗丝木质气的目的。该方法不改变现有梗丝加料工序,能有效减轻梗丝中的木质气,并且生产工艺简单,易操作。
Description
技术领域
本发明属于烟草加工技术领域,涉及一种改善梗丝吸味品质的方法,更具体地说,是涉及一种能有效减轻梗丝木质气的复合料液,以及应用该复合料液减轻梗丝木质气的方法。
背景技术
梗丝中存在的最大问题是木质气较重,而木质气主要由构成细胞壁的木质素燃烧时产生,因此破坏木质素结构是降低木质气的关键技术。
由于采用化学方式处理会对环境产生较大的污染,因此研究人员尝试采用生物技术,如采用能分泌降解木质素酶系的微生物进行作用,或采用碱或酸处理的方式进行。虽已取得一定的效果,但能满足工业化应用的梗丝处理方法相对较少。
研究发现,漆酶能有效作用于木质素。漆酶是单电子氧化还原酶,具有广泛的底物专一性,可氧化的底物包括:酚类及其衍生物、芳胺及其衍生物、芳香羧酸及其衍生物等,在小分子的介体物质存在下,形成的漆酶介体系统,可使得漆酶可氧化的底物范围进一步扩大。另外,漆酶是一种含铜的多酚氧化酶,铜对漆酶有活化作用。Mn2+对漆酶活性也产生重要影响。但如何利用漆酶来降低梗丝中的木质气含量,现有技术中尚未见有成功的报道。
发明内容
本发明的目的在于针对现有技术的不足,提供一种能有效减轻梗丝木质气的复合料液,利用漆酶与香料物质构成的介体系统在作用于梗丝中木质素的同时,提升梗丝感官品质。
本发明的目的还在于提供一种应用该复合料液减轻梗丝木质气的方法。
本发明的目的通过下述技术方案予以实现。
*除非另有说明,本发明中所采用的百分数均为质量百分数。
一种能减轻梗丝木质气的复合料液,其特征在于:将液体漆酶与香料物质按照1∶30的质量比,在4℃条件下混合均匀后制成;其中,所述的香料物质为烟草提取物、梅子提取物、复合果类提取物或人参皂甙中的任意一种,或任意几种的任意比例的混合物。
所述的漆酶为纯化的商品酶,其活性达150U/mL。
所述的复合料液置于4℃条件下保存。
应用所述的复合料液减轻梗丝木质气的方法:将配制好的复合料液采用空气压缩的方式按2.5%的质量比喷洒到已切片的梗丝上,储梗丝3h后,采用气流干燥烘丝,冷却后加香,得到成品梗丝。
与现有技术相比,本发明具有以下突出优点:
(1)本发明结合生物技术及加料技术的特点,构建漆酶介体系统去除木质素,有利梗丝品质的提升;
(2)本发明不改变现有梗丝加工工艺,适合规模化应用;
(3)操作过程简单,能进行工业应用。
附图说明
图1为实施例1中,复合料液处理后梗丝的红外图谱分析(CK为对照样品,E6为实验样品)。
图2为实施例1中,复合料液处理后梗丝的X-衍射图谱分析。
具体实施方式
为了使本发明的目的、技术方案及优点更加清楚明白,以下结合附图和实施例,对本发明作进一步地详细说明。应当理解,此处所描述的具体实施例仅用于解释本发明,并不是对本发明技术方案的限定。
实施例1
称取烟草提取物2g、人参皂甙1g,按照质量比2∶1的比例复配形成复合物3g,后采用蒸馏水稀释至30g,即为香料物质。后称取商业液体漆酶(活性150U/mL)1g,按照1∶30(即漆酶与香料物质的质量比)比例温度为4℃条件下进行搅拌,混合均匀后即为复合料液,置于4℃条件保存待用。
称取未加香加料梗丝50g,先将5g蒸馏水采用微量喷雾器添加到梗丝中,放置20min后,将复合料液按照2.5%(复合料液与梗丝质量的比值)的比例采用微量喷雾器添加到梗丝中,对照选用蒸馏水。后将样品置于22℃、60%的恒温恒湿箱中存放3h后,将梗丝烘干后,分别进行化学成分、红外图谱、X-衍射图谱分析,并进行对比评吸。所得结果如下:
从图1中发现616~1632cm-1谱峰由尖峰变为肩峰,表明与木质素中芳香环相连的C-O被破坏;同时,1505~1512cm-1处的吸收峰有尖峰逐渐成为肩峰且不明显,表明木质素中苯环结构中环被破坏且明显减少。从红外图谱中可侧面反映木质素被降解。
从图2中的X-衍射图谱发现,纤维素的结晶度增加,由于纤维素一般被木质素包裹,当木质素被降解后,纤维素会逐渐暴露,其结晶度将会增加,图2侧面说明木质素有一定的降解。
为进一步明确木质素含量的变化,对样品的细胞壁中木质素、纤维素及半纤维素的含量进行了测定,结果如表1所示:
表1细胞壁中主要成分变化情况
从表1中可以看出,纤维素及半纤维素的含量变化不大。木质素含量从3.66%下降到3.00%,下降了18.03%,说明复合料液对木质素有较好的作用效果。
表2感官评价特征
从表2中感官评价可以看出,添加复合料液的样品木质气明显减轻,同时减少残留。
实施例2
称取梅子提取物5g、复合果类提取物1g,烟草提取物4g,按照质量比5∶1∶4的比例复配形成复合物30g,后采用蒸馏水稀释至300g,即为香料物质。后称取商业液体漆酶(活性150U/mL)10g,按照1∶30(即漆酶与香料物质的质量比)比例温度为4℃条件下进行搅拌,混合均匀后即为复合料液,置于4℃条件保存待用。
在昆明卷烟厂新技改线中的梗丝生产线上进行,不改变原有梗丝加工工艺。在切梗后,梗丝加料时,将所得复合料液按2.5%的比例采用空气压缩进行添加后,柜储3h后,进行气流干燥,后加香,制成成品梗丝,对照样以未加复合料液进行。取样,进行梗丝对比评吸。
表3感官评价特征
从表3中感官评价可以看出,添加复合料液的样品木质气明显减轻。
实施例3
称取烟草提取物30g,后采用蒸馏水稀释至300g,即为香料物质。后称取商业液体漆酶(活性150U/mL)10g,按照1∶30(即漆酶与香料物质的质量比)比例温度为4℃条件下进行搅拌,混合均匀后即为复合料液,置于4℃条件保存待用。
在昆明卷烟厂新技改线中的梗丝生产线上进行,不改变原有梗丝加工工艺。在切梗后,梗丝加料时,将所得复合料液按2.5%的比例采用空气压缩进行添加后,柜储3h后,进行气流干燥,后加香,制成成品梗丝,对照样以未加复合料液进行。取样,进行梗丝对比评吸。
表4感官评价特征
从表4中感官评价可以看出,添加复合料液的样品木质气明显减轻。
以上所述仅为本发明的较佳实施例而已,并不用以限制本发明,凡在本发明的精神和原则之内所作的任何修改、等同替换和改进等,均应包含在本发明的保护范围之内。
Claims (4)
1.一种能减轻梗丝木质气的复合料液,其特征在于:将液体漆酶与香料物质按照1∶30的质量比,在4℃条件下混合均匀后制成;其中,所述的香料物质为烟草提取物、梅子提取物、复合果类提取物或人参皂甙中的任意一种,或任意几种的任意比例的混合物。
2.根据权利要求1所述的能减轻梗丝木质气的复合料液,其特征在于:所述的漆酶为纯化的商品酶,其活性达150U/mL。
3.根据权利要求1所述的能减轻梗丝木质气的复合料液,其特征在于:所述的复合料液置于4℃条件下保存。
4.应用权利要求1所述的复合料液减轻梗丝木质气的方法:将配制好的复合料液采用空气压缩的方式按2.5%的质量比喷洒到已切片的梗丝上,储梗丝3h后,采用气流干燥烘丝,冷却后加香,得到成品梗丝。
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