CN102590462A - Method for evaluating comprehensive quality of medicinal material Polygonum capitatum of Relinqing granules - Google Patents
Method for evaluating comprehensive quality of medicinal material Polygonum capitatum of Relinqing granules Download PDFInfo
- Publication number
- CN102590462A CN102590462A CN2012100446653A CN201210044665A CN102590462A CN 102590462 A CN102590462 A CN 102590462A CN 2012100446653 A CN2012100446653 A CN 2012100446653A CN 201210044665 A CN201210044665 A CN 201210044665A CN 102590462 A CN102590462 A CN 102590462A
- Authority
- CN
- China
- Prior art keywords
- medicinal material
- production
- different places
- major component
- polygonum capilalum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a method for evaluating the comprehensive quality of medicinal material Polygonum capitatum of Relinqing granules, which comprises the following steps: providing Polygonum capitatum medicinal materials from different producing areas; measuring the contents (wt%) of the following parameters of Polygonum capitatum: Quercetin, Quercitrin, gallic acid, extract and general flavone; processing the content of each parameter by software SPSS (Statistic Package for Social Science)17.0; determining the characteristic root and the variance contribution (%) of each main ingredient, and determining a first main ingredient; when the accumulated variance contribution (%) is more than or equal to 75%, determining the F values of all Polygonum capitatum medicinal materials from different producing areas; and determining the comprehensive quality of the Polygonum capitatum medicinal materials from different producing areas according to the size sequence of the F values. The method disclosed by the invention is simple, easy and reliable.
Description
Technical field
The present invention relates to the Chinese crude drug field; Be particularly related to Chinese crude drug quality assessment field; Be specifically related to the method that a kind of medicinal material of polygonum capilalum overall quality is estimated, more particularly be specifically related to a kind of major component and clustering methodology drench the overall quality evaluation of clear particle medicinal material headdress flower knotweed to the heat in the different places of production method.
Background technology
The headdress flower knotweed is dry herb or the aerial part of polygonaceae plant headdress flower knotweed Polygonum capitatum Buch.-Ham.ex D.Don; Record in " the national quality of medicinal material standard of Guizhou Province's Chinese crude drug " version in 2003; Effects such as tool clearing heat and promoting diuresis, anti-inflammation, inducing diuresis for treating strangurtia (Guizhou Province's food and medicine Surveillance Authority. the national quality of medicinal material standard [M] of Guizhou Province's Chinese crude drug. Kweiyang: Guizhou science and technology publishing house, 2003:147).
The headdress flower knotweed is a Guizhou seedling medicine commonly used, is that " six seedlings medicines " and the emphasis that Guizhou Province's modernization of Chinese medicine is given priority to cultivated one of the kind of " the seven big Chinese medicine industrial chains " that develop.Applicant of the present invention in the Shibing, Guizhou and the Kweiyang Wudang District set up headdress flower knotweed GAP base, and through national GAP authentication.
Now relevant headdress flower knotweed GAP kind is planted and the research of aspect such as chemical constitution has obtained certain achievement (Sun Changsheng, Han Jianyu, Yang Jingang; Deng. the environmental quality assessment [J] of headdress flower knotweed GAP planting base. traditional Chinese medicine research and information; 2005,7 (2): 27～30), but the existing quality standard of medicinal material of polygonum capilalum is comparatively simple; Having the common problem of traditional Chinese medicine quality control, is that index is estimated with the single component Quercetin only.Quality evaluating method needs further to improve perfect.
CN101091749A discloses a kind of medicinal material of polygonum capilalum, extract and method of quality control thereof medicinal material of polygonum capilalum wherein and has had the finger-print like HPLC; Contain 13 characteristic peaks; Wherein chromatographic condition is: chromatographic column: and Yi Lite HYPERSIL ODS chromatographic column (4.6mm * 150mm, 5mm); Column temperature: 25 ℃; Moving phase: acetonitrile (A)-0.4% phosphoric acid (B) solution binary gradient elution, percentage by weight is: 0-30%: 100-70%; Flow velocity: 0.8mLmin
-1Detect wavelength: 310nm; Sample size 20 μ L.This invention method of quality control has carried out investigating relatively to different places of production headdress flower knotweed; Made up the finger-print of headdress flower knotweed HPLC; Although this invention is estimated different places of production headdress flower knotweed through finger-print similarity based method software; Yet its conclusion only is the headdress flower knotweed and the common pattern similarity relatively in a certain place of production, and this similarity can not draw the quality good or not between the medicinal material of the different places of production.
CN1481666A discloses a kind of method of planting the headdress flower knotweed, this method comprise seed collecting, breed, cultivate and gather headdress flower knotweed kind method for planting of sport technique segments such as processing.The headdress flower knotweed of this method plantation not only headdress flower knotweed of biological property and grows wild is identical, and its pharmacy characteristic is also identical with wild headdress flower knotweed, and its extract can replace the extract of wild headdress flower knotweed as medicinal.
CN102296118A discloses the method that a kind of dna molecular marker authentication technique is differentiated headdress flower knotweed and Nepal's knotweed; In this invention; The ethnic drug headdress flower knotweed representative with Guizhou is research object, explores and uses the RAPD analytical approach that headdress flower knotweed and Nepal's knotweed are carried out analysis of genetic diversity; And on the experiment basis of RAPD, the RAPD method is converted into the SCAR mark, identify for the medicinal material of headdress flower knotweed and Nepal's knotweed provide accurately and reliably, the method for fast and stable.
Yet in the quality assessment aspect seed selection, the breeding, for example medicinal material of polygonum capilalum is carried out quality assessment with regard to medicinal material of polygonum capilalum, still do not have a kind of suitable method up to now with regard to place of production difference.Therefore, this area still needs the method that a kind of quality to different places of production headdress flower knotweed is carried out comprehensive evaluation.
Summary of the invention
The objective of the invention is to instruct for the quality assessment of aspects such as the quality control of medicinal material of polygonum capilalum and seed selection, breeding, GAP GAP; For example medicinal material of polygonum capilalum is carried out quality assessment, a kind of convenience, suitable, reliable method are provided with regard to place of production difference.The inventor finds with major component and clustering methodology the overall quality of different places of production headdress flower knotweed to be estimated, can be effectively for objective, exactly estimate medicinal material of polygonum capilalum quality and fine provenance seed selection, standardization is produced that reference frame is provided.The present invention is based on this discovery and be accomplished.
For this reason, the invention provides the method that a kind of different places of production medicinal material of polygonum capilalum overall quality is estimated, said headdress flower knotweed can be used as the medicinal material that preparation heat is drenched clear particle, and this method may further comprise the steps:
(1) at least 2 medicinal material of polygonum capilalum from the different places of production is provided;
(2) at least one is selected from the content (wt%) of following parameter: Quercetin, quercitin, gallic acid, extract, general flavone in the said medicinal material of polygonum capilalum of mensuration;
(3) content with step (2) each parameter of surveying uses the SPSS17.0 data processing software to handle, and makes each achievement data standardization;
(4) each w hich is judged, confirmed the major component number, and confirm the characteristic root and the variance contribution ratio % of each major component;
(5) with variance contribution ratio (%) order from big to small major component is sorted, the maximum major component of variance contribution ratio (%) is the 1st major component (being F1);
(6) each major component that sorts to descending order; When the 1st major component (being F1), the 2nd major component (being F2) ..., the variance accumulation contribution rate (%) of this i of i major component (being Fi) major component is more than or equal to 75% o'clock, confirms the F value of every kind of different places of production medicinal material of polygonum capilalum with the following formula account form:
F=G
1* F
1+ G
2* F
2+ ... G
i* F
1... equation 1
Respectively F in the formula
1, F
2..., F
iVariance contribution ratio %;
(7) with F value order from big to small each medicinal material is sorted, the medicinal material of F value big (promptly sorting preceding) is the high medicinal material of overall quality, with the overall quality of this definite different places of production medicinal material of polygonum capilalum of being estimated.
According to the method for the invention, wherein in the step (1), at least 5 medicinal material of polygonum capilalum from the different places of production are provided.In one embodiment, at least 10 medicinal material of polygonum capilalum from the different places of production are provided.In one embodiment, at least 20 medicinal material of polygonum capilalum from the different places of production are provided.In one embodiment, at least 30 medicinal material of polygonum capilalum from the different places of production are provided.In one embodiment, at least 40 medicinal material of polygonum capilalum from the different places of production are provided.It will be appreciated by those skilled in the art that the test article of choosing is many more, then can accurately confirm the better place of production more.Generally speaking, can satisfy the requirement of goal of the invention more than 10 or 10 from the medicinal material of polygonum capilalum in the different places of production; Is those skilled in the art's acceptance of can being satisfied with usually and provide more than 20 or 20 from the medicinal material of polygonum capilalum in the different places of production for the requirement of satisfying goal of the invention.Therefore in a preferred version of the present invention, at least 20 medicinal material of polygonum capilalum from the different places of production are provided.
According to the method for the invention, the medicinal material of polygonum capilalum from the different places of production is to originate from 22～29 ° of north latitude, the medicinal material in 102～110 ° of zones of east longitude described in the step (1).In one embodiment, the medicinal material of polygonum capilalum from the different places of production is to originate from 23～28 ° of north latitude, the medicinal material in 103～109 ° of zones of east longitude described in the step (1).
According to the method for the invention, the medicinal material of polygonum capilalum from the different places of production is the medicinal material that originates from height above sea level 300～1800m zone described in the step (1).In one embodiment, the medicinal material of polygonum capilalum from the different places of production is the medicinal material that originates from height above sea level 300～1600m zone described in the step (1).
According to the method for the invention, measure the content (wt%) of following 5 parameters in the medicinal material of polygonum capilalum in the step (2): Quercetin, quercitin, gallic acid, extract, general flavone.
In an embodiment of the inventive method; Said Quercetin be with reference to " Guizhou Province's Chinese crude drug, national quality of medicinal material standard " (Guizhou Province's food and medicine Surveillance Authority. the national quality of medicinal material standard [M] of Guizhou Province's Chinese crude drug. Kweiyang: Guizhou science and technology publishing house, 2003:147) measure under the headdress flower knotweed item.In one embodiment, the quercetin content of said medicinal material of polygonum capilalum from the different places of production is preferably greater than 0.12, preferably between 0.1～1, preferably between 0.12～0.5 greater than 0.10%.
In an embodiment of the inventive method, said quercitin is with reference to thanking space (Xie Yu, Zhang Liyan; Liang Bin; Measure the content [J] of quercitin in headdress flower knotweed and the clear particle of preparation heat pouring Deng the .HPLC method. CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2009,34 (8): test method 984) is measured.In one embodiment, the quercitin content of said medicinal material of polygonum capilalum from the different places of production is preferably greater than 0.16%, preferably between 0.15～1.5%, preferably between 0.15～1% greater than 0.15%.
In an embodiment of the inventive method, said gallic acid be with reference to thank space (Xie Yu. Guizhou seedling medicine headdress flower knotweed and many index ' s qualities of preparation control method research [D] Kweiyang: department of pharmacy of Guiyang College of Traditional Chinese Medicine, 2009) test method; Is to extract the solvent reflux two hours with water, adopts high performance liquid chromatography, with methanol-water-N, dinethylformamide-glacial acetic acid (1: 95: 3: 1) be moving phase, detection under the 272nm wavelength and mensuration.In one embodiment, the gallic acid content of said medicinal material of polygonum capilalum from the different places of production is preferably greater than 0.12%, preferably between 0.12～1.5%, preferably between 0.12～1.2% greater than 0.1%.
In an embodiment of the inventive method, said headdress flower knotweed extract is that " hot dipping under Chinese pharmacopoeia water-soluble extractives determination method (the appendix X A) item was measured with reference to version in 2010.In one embodiment, the headdress flower knotweed extract content of said medicinal material of polygonum capilalum from the different places of production is preferably greater than 15%, preferably between 15～50%, preferably between 15～30% greater than 10%.
In an embodiment of the inventive method; Said headdress flower knotweed general flavone is with reference to Zhang Liyan (Zhang Liyan; Yang Yuqin, height is explained, etc. the Dynamic variation [J] of general flavone in the different places of production, Guizhou, the different phenological headdress flower knotweed. CHINA JOURNAL OF CHINESE MATERIA MEDICA; 2003,28 (9): test method 889-890) is measured.In one embodiment, the headdress flower knotweed general flavone content of said medicinal material of polygonum capilalum from the different places of production is preferably greater than 0.75%, preferably between 0.75～5%, preferably between 0.75～4% greater than 0.5%.
According to the method for the invention, determined major component number is 5 in the step (4).According to the method for the invention, the variance contribution ratio (%) of the 1st major component of variance contribution ratio (%) maximum is preferably greater than 55%, more preferably greater than 60% greater than 50% in the step (5).According to the method for the invention, the variance contribution ratio (%) of the 2nd major component that variance contribution ratio (%) the 2nd is big in the step (5) is preferably greater than 10%, more preferably greater than 15% greater than 7.5%.According to the method for the invention, the variance contribution ratio (%) of the 3rd major component that variance contribution ratio (%) the 3rd is big in the step (5) is preferably greater than 7.5%, more preferably greater than 10% greater than 5%.According to the method for the invention, in the step (5), variance contribution ratio (%) sum of the 1st major component and the 2nd major component is preferably greater than 75% greater than 70%.
According to the method for the invention, in the step (6), F
1, F
2..., F
iBe respectively to confirm separately by the proper vector of Quercetin, quercitin, gallic acid, extract, five kinds of materials of general flavone.
According to the method for the invention; In the step (7); Further comprise the step of confirming the medicinal material of polygonum capilalum high-quality place of production, this step is: the overall quality according to the determined different places of production of step (7) medicinal material of polygonum capilalum is classified, and the place of production of concentrating with the high-quality medicinal material of polygonum capilalum is as the high-quality place of production.
According to the method for the invention, it also comprises step (8), and this step is to be index with five component contents of headdress flower knotweed, adopts euclidean distance (Euclidean distance) coefficient that different places of production headdress flower knotweed quality index is carried out cluster analysis.According to the present invention, this cluster analysis can verify further that the inventive method step (1) to (7) is for the good and bad accuracy of judging in the medicinal material place of production.
The characteristic that the arbitrary embodiment of the present invention is had is equally applicable to other arbitrary embodiment, as long as they can be not conflicting; Certainly at where applicable each other, necessary words can be done suitably to modify to individual features.All documents that the present invention quoted from, their full content is incorporated this paper by reference into, and if the expressed implication of these documents and the present invention when inconsistent, be as the criterion with statement of the present invention.In addition; Various terms and phrase that the present invention uses have the general sense of well known to a person skilled in the art; Nonetheless; The present invention still hopes at this more detailed explanation and explanation to be done in these terms and phrase, and term of mentioning and phrase are as the criterion with the implication that the present invention was explained if any inconsistent with known implication.
In the present invention, when mentioning %,, be meant w/w percentage like not explanation in addition.
In the present invention, when mentioning quercetin content, quercitin content, gallic acid content, extract content, general flavone content they, they are w/w percentage (wt%), and each is that radix calculates naturally with the medicinal material.
The present invention relates generally to the overall quality evaluation to different places of production headdress flower knotweed of major component and clustering methodology.The quality that the objective of the invention is to comprehensive comparison, the different places of production of evaluation headdress flower knotweed.
In order comprehensively to compare, estimate the quality of different places of production headdress flower knotweed better; The present invention is an index with Quercetin, quercitin, gallic acid, extract, general flavone in an example; Utilization major component and cluster analysis; To the wild and Baoshan, Yunnan in 39 areas such as Guizhou, Yunnan, the medicinal material of polygonum capilalum in Tengchong introducing and planting GAP base in the Shibing; Totally 41 samples carry out Comprehensive Appraisal Study, and combine factors such as collecting location, soil types, height above sea level to inquire into the reason that possibly cause chemical component difference between the headdress flower knotweed of the different places of production.For objective, exactly estimate medicinal material of polygonum capilalum quality and fine provenance seed selection, standardization is produced that reference frame is provided.
The present invention adopts assay methods such as high performance liquid chromatography, ultraviolet spectrophotometry, water-soluble extractives; Quercetin, quercitin, gallic acid, general flavone, extract are measured in the wild and cultivation headdress flower knotweed to 41 of the different places of production; And be index with the content of above-mentioned five index components, utilization major component and cluster analysis are wild and cultivate the headdress flower knotweed and carry out the overall quality evaluation study to 41 of the different places of production.According to measuring the result, the inventor finds that Quercetin, quercitin, gallic acid, extract, general flavone content have notable difference in the headdress flower knotweed of the different places of production, and except that No. 2 samples in Bijie County Liang Yan town, Guizhou Province, all the other samples all reach medicinal requirements.Overall quality with eastern Guizhou medicinal material of polygonum capilalum sample is better.This shows that influenced by the place of production, soil types bigger for contained above-mentioned chemical constitution in the headdress flower knotweed sample, it is less influenced by height above sea level.Wherein, WESTERN GUIZHOU area headdress flower knotweed wild resource is than horn of plenty, but its index property composition quercetin content and overall quality are lower than eastern Guizhou area and other provinces sample; Eastern Guizhou area headdress flower knotweed quercetin content is higher, overall quality is better, has verified that headdress flower knotweed base build the rationality of Shibing, the southeast of Guizhou Province in.The inventive method is that seed selection, GAP standardization objective, that synthetically estimate quality and the fine provenance of medicinal material of polygonum capilalum are produced important reference frame is provided.
Use the SPSS17.0 data processing software in the inventive method step (3), this data processing software can the free download from the internet, can also buy or download from its official website from U.S. SPSS Inc. company.It will be apparent to those skilled in the art that; For realizing the object of the invention; When for example in the inventive method step (3), carrying out data processing; These algorithms can be employed in and calculate other software for calculation or computing method similar or identical on the principle, as long as can be realized the object of the invention with the SPSS17.0 data processing software.Therefore; Although use the SPSS17.0 data processing software in the inventive method step (3); Yet other software for calculation or computing method similar or identical with the SPSS17.0 data processing software on the calculating principle will prolonged and use to protection scope of the present invention, as long as these algorithms can be realized the object of the invention.
Those skilled in the art know, principal component analysis (PCA) (Principal Component Analysis, PCA) be a kind of with a plurality of variablees through linear transformation to select a kind of multivariate statistical analysis method of less number significant variable.Claim principal component analysis again.In actual subject,, a lot of relevant therewith variablees (or factor) are often proposed, because each variable is all reflecting some information of this problem in varying degrees for the multianalysis problem.Principal component analysis (PCA) is at first introduced nonrandom variable by the K. Pearson, and the H. Hotelling is generalized to the method the situation of random vector thereafter.The size of information is weighed with sum of squares of deviations or variance usually.
With this multivariable problem of statistical analysis technique research the time, the variable number will increase the complicacy of problem too much.People hope that naturally the variable number is less and information that obtain is more.In a lot of situations, be that certain correlationship is arranged between the variable, when between two variablees certain correlationship being arranged, the information that can be interpreted as these these problems of two variablees reflection has certain overlapping.Principal component analysis (PCA) is all variablees for original proposition, sets up the least possible new variables, makes that these new variables are incoherent in twos, and these new variables keep original information as far as possible at the message context of reflection problem.
Manage primal variable is reassembled into one group of new irrelevant mutually several generalized variable; Therefrom can take out simultaneously several less generalized variables according to actual needs and reflect that as much as possible the statistical method of the information of primal variable is called principal component analysis (PCA) or claims principal component analysis, also be a kind of method of handling dimensionality reduction on the mathematics.
Principal component analysis (PCA) is to manage original numerous certain correlativitys (such as P index) that have, and is reassembled into one group of new overall target that has nothing to do mutually and replaces original index.Common mathematical processing is done linear combination with an original P index exactly, as new overall target.The most classical way uses the variance of F1 (first linear combination of choosing, i.e. first overall target) to express exactly, and promptly Var (F1) is big more, and the information that expression F1 comprises is many more.Therefore the F1 that in all linear combination, chooses should be that variance is maximum, so title F1 is a first principal component.If first principal component is not enough to represent the information of an original P index, consider that again choosing F2 promptly selects second linear combination, in order to reflect original information effectively; The F1 existing information does not just need to appear among the F2 again, with mathematical linguistics express be exactly require Cov (F1, F2)=0; Claim that then F2 is a Second principal component; The rest may be inferred can construct the 3rd, the 4th ..., P major component.
In principal component analysis (PCA), can use classical equation:
Fp＝a1iZX1+a2iZX2+......+apiZXp
A1i wherein, a2i ..., api (i=1; ..., m) be the pairing proper vector of eigenwert of the covariance matrix ∑ of X, ZX1, ZX2; ..., ZXp is the value of original variable through standardization, because in practical application, often exists the dimension of index different; So before calculating, must eliminate the influence of dimension earlier, and with the raw data standardization, just there is the dimension influence in the data that this paper adopted.
A=(aij) p * m=(a1, a2 ... am), Rai=λ iai, R are correlation matrix, λ i, ai are corresponding eigenwert and unit character vector, λ 1 >=λ 2 >=... >=λ p >=0.
Carry out principal component analysis (PCA) and comprise that mainly step is following: achievement data standardization (SPSS software automatically performs); W hich is judged; Confirm major component number m; Major component Fi expression formula; Major component Fi name.
In addition, the also basic thought of the principal component analysis (PCA) of write up, ultimate principle, main effect, calculation procedure, applied analysis and classical case (referring to the http://wiki.mbalib.com/wiki/%E4%B8%BB%E6%88%90%E5%88%86%E5%88%8 6%E6%9E%90%E6%B3%95s) such as application of PCA in the beer flavor evaluation analysis for example in MBA think tank encyclopaedia
Further, the clustering methodology that those skilled in the art know is desirable multivariate statistics technology, mainly contains hierarchical cluster method and iteration clustering procedure.Cluster analysis, cluster analysis are also claimed in cluster analysis, are a kind of multivariate statistical methods of research classification.
The inventor finds to use PCA or itself and clustering methodology to make up; With Quercetin, quercitin, gallic acid, extract, five parameters of general flavone is index; Find the medicinal material that the medicinal material of polygonum capilalum high-quality place of production that analytical approach of the present invention thus obtains produces, the medicinal material of producing in other area that aspect overall quality, compares is obviously more excellent.
Description of drawings
That Fig. 1 describes is the cluster analysis figure of 41 different places of production headdress flower knotweed samples, and it is a dendrogram (between group) of using the mean contact, and lateral coordinates is represented the spacing of the calibrating combination of bunching among the figure, and along slope coordinate representes that the instance indication numbers.
What Fig. 2 described is the scatter diagram of 41 different places of production headdress flower knotweed samples; Among the figure; The elliptical region that comprises samples such as numbering No. 2, No. 9, No. 34 is the A group; The elliptical region that comprises samples such as numbering No. 4, No. 5, No. 10 is the B group, and the elliptical region that comprises samples such as numbering No. 3, No. 14, No. 18 is the C group, and the elliptical region that comprises samples such as numbering No. 23, No. 24, No. 25 is that D organizes.
Among Fig. 2, horizontal ordinate " REGR factor score 2for analysis 1 " expression is used to analyze 1 REGR factor score 2; Ordinate " REGR factor score 1 for analysis 1 " expression is used to analyze 1 REGR factor score 1.
Embodiment
Can further describe the present invention through following embodiment, yet scope of the present invention is not limited to following embodiment.One of skill in the art can understand, and under the prerequisite that does not deviate from the spirit and scope of the present invention, can carry out various variations and modification to the present invention.The present invention carries out generality and/or concrete description to the material and the test method that are used in the test.Though for realizing that employed many materials of the object of the invention and method of operating are well known in the art, the present invention still does to describe in detail as far as possible at this.
One, testing tool and reagent
Instrument: Agilent 1100 high performance liquid chromatographs (vacuum degassing machine, automatic sampler, quaternary pump, DAD detecting device), Agilent 1100 liquid chromatographic system chem workstations.
Reagent: Quercetin reference substance (lot number: 100081-200406; Assay usefulness), gallic acid reference substance (lot number: 110831-200302; Assay usefulness), quercitin reference substance (lot number: 111538-200403; Assay with), rutin (lot number: 110725-200513, assay with) provides by Nat'l Pharmaceutical & Biological Products Control Institute.Methyl alcohol, tetrahydrofuran, acetonitrile are chromatographically pure; Water is pure water; It is pure that other reagent is analysis.
Medicinal material of polygonum capilalum: 41 different places of production (table 1 sees below) medicinal material of polygonum capilalum is from adopting.Identify through Guiyang College of Traditional Chinese Medicine, be the dry aerial parts of polygonaceae plant headdress flower knotweed Polygonum capitatum Buch.-Ham.exD.Don.Each kind ground sample is preserved in 40 ℃ of oven dry, pulverizing, sealing, sees table 1.
Two, the assay method of evaluating
Measure Quercetin, quercitin, gallic acid, extract, content of total flavone (wt%) in 41 kinds of medicinal material of polygonum capilalum with following method respectively.
1, the mensuration of headdress flower knotweed Quercetin:
Reference " Guizhou Province's Chinese crude drug, national quality of medicinal material standard " (Guizhou Province's food and medicine Surveillance Authority. the national quality of medicinal material standard [M] of Guizhou Province's Chinese crude drug. Kweiyang: Guizhou science and technology publishing house, 2003:147) measure under the headdress flower knotweed item, the result sees table 1.
2, the mensuration of headdress flower knotweed quercitin:
With reference to thank space (Liang Bin waits the .HPLC method to measure the content [J] that headdress flower knotweed and preparation heat are drenched quercitin in the clear particle for Xie Yu, Zhang Liyan. CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2009,34 (8): test method 984) is measured, and the result sees table 1.
3, the mensuration of headdress flower knotweed gallic acid
With reference to thank space (Xie Yu. Guizhou seedling medicine headdress flower knotweed and many index ' s qualities of preparation control method research [D] Kweiyang: department of pharmacy of Guiyang College of Traditional Chinese Medicine, 2009) test method; With water is to extract the solvent reflux two hours, adopts high performance liquid chromatography, with methanol-water-N, and dinethylformamide-glacial acetic acid (1: 95: 3: 1) be moving phase, under the 272nm wavelength, detect that the result sees table 1.
4, the mensuration of headdress flower knotweed extract
" hot dipping under Chinese pharmacopoeia water-soluble extractives determination method (the appendix X A) item was measured, and the result sees table 1 with reference to version in 2010.
5, the mensuration of headdress flower knotweed general flavone
With reference to Zhang Liyan (height is explained for Zhang Liyan, Yang Yuqin, etc. the Dynamic variation [J] of general flavone in the different places of production. Guizhou, the different phenological headdress flower knotweed. CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2003,28 (9): test method 889-890) is measured, the result sees table 1.
6, data processing method
Use the SPSS17.0 data processing software, the computing machine that quercetin content (%), quercitin content (%), gallic acid content (%), extract (%), general flavone content (%) input of the headdress flower knotweed in the source of measuring, the various different places of production is equipped with this software is analyzed.The proper vector of each content parameter of the characteristic root of acquisition first principal component, variance contribution ratio % etc. and mensuration.
Three, the result of PCA and analysis
1, different place of production headdress flower knotweed is respectively investigated the assay result of parameter
Use above method, the assay result that different places of production headdress flower knotweed is respectively investigated parameter sees table 1 respectively.
Table 1: different places of production headdress flower knotweed is respectively investigated the assay result and the major component ranking results of parameter
2, principal component analysis (PCA) is used for the quality assessment of headdress flower knotweed
Content with five indexs of headdress flower knotweed is index, and the utilization principal component analysis (PCA) is carried out assay to 41 different places of production headdress flower knotweeds.Through the SPSS computed in software, the result finds that the 1st major component variance contribution ratio is 61.710%, and the Quercetin contribution rate is maximum, and proper vector is 0.931; The 2nd major component variance contribution ratio is 17.886%, and the gallic acid contribution rate is maximum, and proper vector is 0.816.Preceding two major component contribution rate of accumulative total of variance reach 79.595%.Those skilled in the art it has been generally acknowledged that, when contribution rate of accumulative total reach 75% think when above satisfied (Wang Fang, etc. the similarities and differences of principal component analysis (PCA) and factorial analysis relatively reach application [J]. statistical education, 2003 (5): 141).Therefore, the 1st, the 2nd major component can reflect general status basically, reaches the purpose of dimensionality reduction.According to proper vector, can obtain major component linear combination expression formula and be:
F＝0.6171F
1+0.1789F
2，
Wherein, F
1=0.931X1+0.846X2+0.523X3+0.636X4+0.908X5,
F
2＝0.037X1-0.255X2+0.816X3-0.402X4+0.011X5)
Press the function to calculate the value of the principal component of the comprehensive and sort, get 41 heads Polygonum sample quality comprehensive evaluation value (Zhang Nan, Zhao Guowei, et al. Principal component analysis and cluster analysis for microcrystalline cellulose classification [J]. of Traditional Chinese Medicine, 2011,17 (19) :4-8; Cui safflower, high juvenile Heng, Cai Hongfei, such as principal component analysis study on the origin of the best bergamot [J]. Guangzhou University of Chinese Medicine, 2009,29 (3 ): 281; Jiao Yang, Yin Haibo, etc. Based on ICP-MS for the different areas of inorganic elements in Taipa first principal component analysis and cluster analysis [J]. of Traditional Chinese Medicine, 2011,17 (17) :68-72 ), the results are shown in Table 1, the right-most one.The direction of Quercetin, quercitin, extract, general flavone proper vector is identical; With the gallic acid proper vector in the opposite direction (Zhang Nan, State of Zhao is towering, etc. principal component analysis (PCA) and clustering methodology are used for microcrystalline cellulose classification [J]. Chinese experimental pharmacology of traditional Chinese medical formulae magazine; 2011,17 (19): 4-8).It is thus clear that be positive correlation between Quercetin, quercitin, extract, the general flavone, all become negative correlativing relation respectively with gallic acid.The result sees table 2.
Table 2: variable describe, in general terms
| Project | F1 | F2 | F3 | F4 | F5 |
| Characteristic root | 0.6171 | 0.17886 | 0.13353 | 0.0480 | 0.0225 |
| Variance contribution ratio % | 61.71 | 17.886 | 13.353 | 4.802 | 2.249 |
| Variance accumulation contribution rate % | 61.71 | 79.595 | 92.494 | 97.751 | 100 |
| Quercetin feature vector, |
0.931 | 0.037 | -0.224 | 0.128 | -0.254 |
| Quercitin feature vector, |
0.846 | -0.255 | -0.394 | 0.153 | 0.203 |
| Gallic acid feature vector, X 3 | 0.523 | 0.816 | 0.206 | 0.106 | 0.079 |
| Extract feature vector, |
0.636 | -0.402 | 0.648 | 0.119 | 0.010 |
| General flavone feature vector, |
0.908 | 0.011 | 0.024 ＊ | -0.418 | 0.019 |
Four, the cluster analysis of headdress flower knotweed quality assessment
Content with five indexs of headdress flower knotweed is index, adopts euclidean distance (Euclidean distance) coefficient that different places of production headdress flower knotweed quality index is carried out cluster analysis.Analysis result is seen Fig. 1 and Fig. 2 respectively.That wherein Fig. 1 describes is the cluster analysis figure of 41 different places of production headdress flower knotweed samples, and it is a dendrogram (between group) of using the mean contact, and lateral coordinates is represented the spacing of the calibrating combination of bunching among the figure, and along slope coordinate representes that the instance indication numbers.What Fig. 2 described is the scatter diagram of 41 different places of production headdress flower knotweed samples; Among the figure; The elliptical region that comprises samples such as numbering No. 2, No. 9, No. 34 is the A group; The elliptical region that comprises samples such as numbering No. 4, No. 5, No. 10 is the B group, and the elliptical region that comprises samples such as numbering No. 3, No. 14, No. 18 is the C group, and the elliptical region that comprises samples such as numbering No. 23, No. 24, No. 25 is that D organizes.
According to Figure 1, Figure 2 and Table 1, Table 2, the results, when the threshold is 15, the 41 heads of different origin Polygonum samples were clustered into two groups: Sichuan Xingwen County stone sea town, Guizhou Panxian LIU Guan Town, Bo Wang Xing Wen County, Sichuan town, close mountains Qianxi County, Guizhou, Guangxi Tianlin County, Lee Weeks Township No. 1, No. 2, Guizhou Leishan West Town, Chongqing Nanchuan District Sanhui games, Sichuan Gao County shazhen, Sichuan Xingwen Ancient Song Town, County, Guizhou Xingren horse jiatun, Guizhou Long Lane County, Bay Village, state-owned forest Leye County, Guangxi, Guizhou Luodian Rosu Township, Hunan Guzhang County Old Town, Guizhou Kaili City, three in town Yunnan Shizong County High Liangzhen, Pingxiang, Guangxi Tianlin waves, Guangxi Tianlin County, Lee Township, on the 2nd week, Bing introduced in Baoshan, Yunnan, Guizhou Shiqian in Nanba, Guizhou Leishan West Town on the 1st, Guizhou Leishan Shan Town, Yunnan Shizong five Xiang clustered into group I, and the remaining samples were clustered into Group II.
Result in conjunction with principal component analysis (PCA) and cluster analysis can find out that mostly the I group is eastern Guizhou area and other provinces sample, and the overall quality evaluation of estimate sorts between 1-23, and the overall quality evaluation of estimate is higher; Mostly the II group is the sample in WESTERN GUIZHOU area, and the overall quality evaluation of estimate sorts between 24-41, and the overall quality evaluation of estimate is lower.The sample in WESTERN GUIZHOU area is more, has reflected this regional headdress flower knotweed wild resource than horn of plenty, but through the overall quality evaluation, this district's headdress flower knotweed overall quality evaluation of estimate is lower, is starkly lower than eastern Guizhou area and other provinces sample.
Five, the correlativity evaluation of headdress flower knotweed chemical index and geographic distribution
For further studying the relation between headdress flower knotweed chemical constitution and the geographic distribution, use SPSS17.0 correlation analysis is carried out in the five fingers mark content and its location geographic distribution in the headdress flower knotweed.The result shows: all the other four-indexes and the different places of production, soil types correlativity are remarkable except that quercitin.Explain that the different places of production, soil types have certain influence to headdress flower knotweed chemical index, the correlativity of sea level elevation and extract is remarkable.The result sees table 3
Table 3: the correlativity in headdress flower knotweed chemical index and geographic position
| Geographic distribution | Gallic acid | Quercetin | Quercitin | Extract | General flavone |
| Different sample ground | -.654 ＊ | -.390 ＊ | -.049 | -.362 ＊ | -.372 ＊ |
| Height above sea level | -.108 | -.033 | .085 | -.406 ＊ | -.023 |
| Soil types | -.394 ＊ | -.054 ＊ | -.655 | -.364 ＊ | -.374 ＊ |
"
*" to be illustrated under 95% the degree of confidence correlativity remarkable
With the principal component analysis (PCA) is the basis, utilization SPSS17.0 software, and the 1st and the 2nd principal component scores coefficient of each sampled point generates scatter diagram, and the result sees Fig. 2.The result shows: A, B are other provinces sample mostly two groups, and Quercetin, quercitin, extract, general flavone content are all higher, and gallic acid content is lower.C, D are sample in the Guizhou Province mostly two groups, and gallic acid content is higher, and Quercetin, quercitin, extract, general flavone content are lower.And C, two groups of samples of D comparatively concentrate on the west area, Guizhou Province, and forming this kind gathering maybe be relevant with different geographical environments.The headdress flower knotweed of this experiment picks up from Guizhou, Sichuan, Chongqing, Yunnan, Guangxi, 41 areas of Hunan six provinces.Except that the Hunan, all being positioned at area, the Yunnan-Guizhou Plateau, is the second level ladder of China's topography profile, and low the making of Xi Gaodong can be climbed from the east orientation west from Pacific warm moist air, brings large tracts of land precipitation for this area, forms the subtropics monsoon climate.Headdress flower knotweed seed is with people and animals and anemochory; The formed geographic isolation of the typical karst landform in Guizhou; Maybe to headdress flower knotweed germ plasm resource exchange produced certain influence (Cui Honghua, Gao Youheng, Cai Hongfei etc. principal component analysis (PCA) is to the discussion [J] in the best place of production of Buddha's hand. Traditional Chinese Medicine University Of Guangzhou's journal; 2009,29 (3): 281).Headdress flower knotweed sample is at this point distinguished the gathering of comparatively concentrating, and can infer that geographical environment is bigger to the medicinal material of polygonum capilalum influence.
Six, evaluating is to result's influence
For verifying the validity of the method that different places of production medicinal material of polygonum capilalum overall quality is estimated, the inventor carries out the principal component analysis (PCA) evaluation to above-mentioned 41 different places of production headdress flower knotweeds.In a SPSS17.0 data processing software analysis example; The investigation parameter is: quercitin, gallic acid, extract, general flavone; The result is in these 41 samples, and the ordering of the major component evaluation result of Sankeshu Town, Kaili City, Guizhou Province, two samples in Luo Su township, Luodian County, Guizhou Province is respectively 26 and 19.In another SPSS17.0 data processing software analysis example; The investigation parameter is: Quercetin, quercitin, extract, general flavone; The result is in these 41 samples, and the ordering of the major component evaluation result of Sankeshu Town, Kaili City, Guizhou Province, two samples in Luo Su township, Luodian County, Guizhou Province is respectively 17 and 33.In another SPSS17.0 data processing software analysis example; The investigation parameter is: Quercetin, gallic acid, general flavone three; The result is in these 41 samples, and the ordering of the major component evaluation result of Sankeshu Town, Kaili City, Guizhou Province, two samples in Luo Su township, Luodian County, Guizhou Province is respectively 14 and 29.
Seven, the checking of different place of production medicinal material of polygonum capilalum overall quality evaluation result
Get the medicinal material in two the eastern Guizhou areas in Sankeshu Town, Kaili City, Guizhou Province and Luo Su township, Luodian County, Guizhou Province in the table 1, and chicken house township, Shuicheng County, Guizhou, the regional medicinal material of two WESTERN GUIZHOU in Bai Guo town, Pan county, Guizhou, the conventional method according to following headdress flower knotweed prepares extract powder respectively: get headdress flower knotweed 220kg; Boiling 2 times; Each 1.5 hours, collecting decoction filtered; Filtrating is concentrated into the clear cream that relative density is 1.10 ± 0.05 (85 ℃); Filter, spray drying to moisture makes 4 kinds of extract powders in the scope of 5-8%.Measure general flavone content (wt%) in medicinal extract yield (%) and the extract powder, latter's percentage is to be the percentage of radix in the medicinal material.
The result shows that for the medicinal extract yield, the medicinal material in two eastern Guizhou areas is respectively 13.4% and 12.1%, and the medicinal material in two WESTERN GUIZHOU areas is respectively 7.9% and 9.2%; For general flavone content, the medicinal material in two eastern Guizhou areas is respectively 3.62% and 3.27%, and the medicinal material in two WESTERN GUIZHOU areas is respectively 0.94% and 1.18%.
The present invention uses principal component analysis (PCA) with cluster analysis 41 different places of production headdress flower knotweeds to be carried out assay.The result shows that Quercetin, quercitin, gallic acid, extract, general flavone content have notable difference in the medicinal material of polygonum capilalum of the different places of production, and is wherein better with sample overall qualities such as Sankeshu Town, Kaili City, Guizhou Province, Luo Su townshiies, Luodian County, Guizhou Province.With the single index of Quercetin; Except that the sample quercetin content in No. 2, Bijie County Liang Yan town, Guizhou Province be lower than 0.1% (Guizhou Province's food and medicine Surveillance Authority. the national quality of medicinal material standard [M] of Guizhou Province's Chinese crude drug. Kweiyang: Guizhou science and technology publishing house; 2003:147), all the other samples all reach medicinal requirements.Headdress flower knotweed initial quality standard is comparatively simple, and the method that suggestion adopts the evaluation of many index comprehensives to combine with drug action is carried out quality control to the headdress flower knotweed.
Know, and Wang Xiangpei (Wu Hongmei waits .HPLC to measure the content [J] of quercitin in the headdress flower knotweed in the different places of production for Yang Liyong, Wang Xiangpei. Guiyang College of Traditional Chinese Medicine's journal, 2009,31 (4): 67-68; Wang Xiangpei; Wan Deguang; Wang Qiang waits .HPLC to measure the content [J] of gallic acid in the headdress flower knotweed in the different places of production. West China pharmaceutical journal, 2007; 204～205) etc. 22 (2): measure the content of quercitin, gallic acid in the headdress flower knotweed in the different places of production with HPLC, draw in the headdress flower knotweed of Shibing, Guizhou that quercitin content is higher, gallic acid content is higher in the Jianhe headdress flower knotweed of Guizhou.Zhou Tao (Zhou Tao; Ai Qiang; Wang Yanjun, etc. based on the content analysis [J] of gallic acid in the different geographical provenance headdress flower knotweeds. Chinese experimental pharmacology of traditional Chinese medical formulae magazine, 2011; 17 (1): 49-52) grade is analyzed the content of gallic acid in the different geographical provenance headdress flower knotweeds, and drawing eastern Guizhou area gallic acid content will be a little more than the west area.This paper carries out comprehensive evaluation with many indexs to the headdress flower knotweed first, and to draw Kerry sample overall quality better, and this area belongs to the eastern Guizhou area, and the result is consistent with bibliographical information.The national GAP of headdress flower knotweed just build in the base Shibing County, eastern Guizhou area in; This result of study has further been verified the correctness of base addressing; The medicinal material of polygonum capilalum overall quality evaluation of estimate ordering in the Baoshan, Yunnan, the Tengchong introducing and planting GAP base in the Shibing is 10,27; Be higher than most of natural crude drugs, visible cultivation quality amount is comparatively stable, controlled.Suggestion can be greatly developed the plantation of headdress flower knotweed from now in the eastern Guizhou area.And area, Luodian sample was not seen bibliographical information in the past for gathering first, should further carry out follow-up investigation research to this regional headdress flower knotweed.
The present invention uses correlation analysis, and chemical constitution receives the influence of the place of production and soil types bigger in the headdress flower knotweed sample, and height above sea level is less to the chemical composition content influence of headdress flower knotweed.Therefore, the incidence relation between effective constituent and the habitat is worth further research, and this has practical significance for the quality that improves the cultivation article.
Claims (17)
1. method that different places of production medicinal material of polygonum capilalum overall qualities is estimated, this method may further comprise the steps:
(1) at least 2 medicinal material of polygonum capilalum from the different places of production is provided;
(2) at least one is selected from the content (wt%) of following parameter: Quercetin, quercitin, gallic acid, extract, general flavone in the said medicinal material of polygonum capilalum of mensuration;
(3) content with step (2) each parameter of surveying uses the SPSS17.0 data processing software to handle, and makes each achievement data standardization;
(4) each w hich is judged, confirmed the major component number, and confirm the characteristic root and the variance contribution ratio % of each major component;
(5) with variance contribution ratio (%) order from big to small major component is sorted, the maximum major component of variance contribution ratio (%) is the 1st major component (being F1);
(6) each major component that sorts to descending order; When the 1st major component (being F1), the 2nd major component (being F2) ..., the variance accumulation contribution rate (%) of this i of i major component (being Fi) major component is more than or equal to 75% o'clock, confirms the F value of every kind of different places of production medicinal material of polygonum capilalum with the following formula account form:
F=G
1* F
1+ G
2* F
2+ ... G
i* F
i... equation 1
Respectively F in the formula
1, F
2..., F
iVariance contribution ratio %;
(7) with F value order from big to small each medicinal material is sorted, the medicinal material of F value big (promptly sorting preceding) is the high medicinal material of overall quality, with the overall quality of this definite different places of production medicinal material of polygonum capilalum of being estimated.
2. at least 5, at least 10, at least 20, at least 30 or at least 40 medicinal material of polygonum capilalum from the different places of production are provided according to the process of claim 1 wherein in the step (1).
3. according to the process of claim 1 wherein that the medicinal material of polygonum capilalum from the different places of production is to originate from 22～29 ° of north latitude, the medicinal material in 102～110 ° of zones of east longitude described in the step (1).
4. according to the process of claim 1 wherein that the medicinal material of polygonum capilalum from the different places of production is the medicinal material that originates from height above sea level 300～1800m zone described in the step (1).
5. according to the process of claim 1 wherein the middle content (wt%) of measuring following 5 parameters in the medicinal material of polygonum capilalum of step (2): Quercetin, quercitin, gallic acid, extract, general flavone.
6. according to the process of claim 1 wherein that the quercetin content of said medicinal material of polygonum capilalum from the different places of production greater than 0.10%, is preferably greater than 0.12, preferably between 0.1～1, preferably between 0.12～0.5.
7. according to the process of claim 1 wherein that the quercitin content of said medicinal material of polygonum capilalum from the different places of production greater than 0.15%, is preferably greater than 0.16%, preferably between 0.15～1.5%, preferably between 0.15～1%.
8. according to the process of claim 1 wherein that the gallic acid content of said medicinal material of polygonum capilalum from the different places of production greater than 0.1%, is preferably greater than 0.12%, preferably between 0.12～1.5%, preferably between 0.12～1.2%.
9. according to the process of claim 1 wherein that the headdress flower knotweed extract content of said medicinal material of polygonum capilalum from the different places of production greater than 10%, is preferably greater than 15%, preferably between 15～50%, preferably between 15～30%.
10. according to the process of claim 1 wherein that the headdress flower knotweed general flavone content of said medicinal material of polygonum capilalum from the different places of production greater than 0.5%, is preferably greater than 0.75%, preferably between 0.75～5%, preferably between 0.75～4%.
11. according to the process of claim 1 wherein that the variance contribution ratio (%) of the 1st major component that variance contribution ratio (%) in the step (5) is maximum greater than 50%, is preferably greater than 55%, more preferably greater than 60%.
12. according to the process of claim 1 wherein that the variance contribution ratio (%) of the 2nd major component that variance contribution ratio (%) in the step (5) the 2nd is big greater than 7.5%, is preferably greater than 10%, more preferably greater than 15%.
13. according to the process of claim 1 wherein that the variance contribution ratio (%) of the 3rd major component that variance contribution ratio (%) in the step (5) the 3rd is big greater than 5%, is preferably greater than 7.5%, more preferably greater than 10%.
14. according to the process of claim 1 wherein that in the step (5), variance contribution ratio (%) sum of the 1st major component and the 2nd major component is preferably greater than 75% greater than 70%.
15. according to the process of claim 1 wherein in the step (6) F
1, F
2..., F
iBe respectively to confirm separately by the proper vector of Quercetin, quercitin, gallic acid, extract, five kinds of materials of general flavone.
16. method according to claim 1; Wherein in the step (7); Further comprise the step of confirming the medicinal material of polygonum capilalum high-quality place of production; This step is: the overall quality according to the determined different places of production of step (7) medicinal material of polygonum capilalum is classified, and the place of production of concentrating with the high-quality medicinal material of polygonum capilalum is as the high-quality place of production.
17. according to the method for claim 1, it also comprises step (8), this step is to be index with five component contents of headdress flower knotweed, adopts euclidean distance (Euclidean distance) coefficient that different places of production headdress flower knotweed quality index is carried out cluster analysis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100446653A CN102590462A (en) | 2012-02-27 | 2012-02-27 | Method for evaluating comprehensive quality of medicinal material Polygonum capitatum of Relinqing granules |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100446653A CN102590462A (en) | 2012-02-27 | 2012-02-27 | Method for evaluating comprehensive quality of medicinal material Polygonum capitatum of Relinqing granules |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102590462A true CN102590462A (en) | 2012-07-18 |
Family
ID=46479392
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012100446653A Pending CN102590462A (en) | 2012-02-27 | 2012-02-27 | Method for evaluating comprehensive quality of medicinal material Polygonum capitatum of Relinqing granules |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102590462A (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103308617A (en) * | 2013-06-03 | 2013-09-18 | 合肥今越制药有限公司 | Fingerprint spectrum detection method for postnatal Gongtai granules |
| CN106568910A (en) * | 2015-10-10 | 2017-04-19 | 成都中医药大学 | Construction method of traditional Chinese medicine integrated pharmacokinetic model |
| CN107561035A (en) * | 2017-07-06 | 2018-01-09 | 成都中医药大学 | A kind of detection method in commercially available Radix Notoginseng powder powder position source |
| CN108627641A (en) * | 2018-04-28 | 2018-10-09 | 璞晞（广州）生物免疫技术有限公司 | The check and evaluation method and kit of hepatopathy T cell function |
| CN110057958A (en) * | 2019-05-21 | 2019-07-26 | 南开大学 | By having effects that the method for the active constituent content evaluation quality grade of Ca2+ overloading in detection Radix Angelicae Sinensis medicinal material |
| CN110095550A (en) * | 2019-06-12 | 2019-08-06 | 青岛农业大学 | A kind of method in the auxiliary identification of corn place of production |
| CN111358839A (en) * | 2020-04-26 | 2020-07-03 | 贵州威门药业股份有限公司 | Formula granules of polygonum capitatum and preparation method thereof |
| CN113567639A (en) * | 2021-07-13 | 2021-10-29 | 中国食品药品检定研究院 | Comprehensive evaluation method for quality of traditional Chinese medicinal materials |
| CN113720931A (en) * | 2021-08-19 | 2021-11-30 | 遂昌县森林王中药材专业合作社 | Quality evaluation method of radix tetrastigme medicinal material |
| CN114846327A (en) * | 2019-12-20 | 2022-08-02 | 日本电信电话株式会社 | Determination system, verification device, and determination method |
| CN115128226A (en) * | 2022-07-19 | 2022-09-30 | 四川农业大学 | Sichuan tangerine peel quality determination method and planting method based on producing area and effective components |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100535655C (en) * | 2006-06-22 | 2009-09-02 | 成都中医药大学 | Medicinal material of polygonum capilalum, extractive, and quality control method |
-
2012
- 2012-02-27 CN CN2012100446653A patent/CN102590462A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100535655C (en) * | 2006-06-22 | 2009-09-02 | 成都中医药大学 | Medicinal material of polygonum capilalum, extractive, and quality control method |
Non-Patent Citations (5)
| Title |
|---|
| 严辉等: "我国不同产地当归药材质量的分析与评价", 《中草药》 * |
| 多杰扎西: "青海省大黄中微量元素的主成分分析", 《安徽农业科学》 * |
| 杨铭: "综合主成分分析用于黄芪药材的质量评价", 《数理医药学杂质》 * |
| 王文燕: "白芍的HPLC指纹图谱及模式识别研究", 《中药草》 * |
| 赵焕新等: "头花蓼化学成分及其分析方法研究进展", 《食品与药品》 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103308617A (en) * | 2013-06-03 | 2013-09-18 | 合肥今越制药有限公司 | Fingerprint spectrum detection method for postnatal Gongtai granules |
| CN106568910A (en) * | 2015-10-10 | 2017-04-19 | 成都中医药大学 | Construction method of traditional Chinese medicine integrated pharmacokinetic model |
| CN107561035A (en) * | 2017-07-06 | 2018-01-09 | 成都中医药大学 | A kind of detection method in commercially available Radix Notoginseng powder powder position source |
| CN108627641A (en) * | 2018-04-28 | 2018-10-09 | 璞晞（广州）生物免疫技术有限公司 | The check and evaluation method and kit of hepatopathy T cell function |
| CN110057958A (en) * | 2019-05-21 | 2019-07-26 | 南开大学 | By having effects that the method for the active constituent content evaluation quality grade of Ca2+ overloading in detection Radix Angelicae Sinensis medicinal material |
| CN110057958B (en) * | 2019-05-21 | 2021-03-02 | 南开大学 | Method for evaluating quality grade by detecting content of active ingredients with calcium antagonistic effect in angelica sinensis medicinal material |
| CN110095550A (en) * | 2019-06-12 | 2019-08-06 | 青岛农业大学 | A kind of method in the auxiliary identification of corn place of production |
| CN114846327A (en) * | 2019-12-20 | 2022-08-02 | 日本电信电话株式会社 | Determination system, verification device, and determination method |
| CN111358839A (en) * | 2020-04-26 | 2020-07-03 | 贵州威门药业股份有限公司 | Formula granules of polygonum capitatum and preparation method thereof |
| CN113567639A (en) * | 2021-07-13 | 2021-10-29 | 中国食品药品检定研究院 | Comprehensive evaluation method for quality of traditional Chinese medicinal materials |
| CN113567639B (en) * | 2021-07-13 | 2023-05-16 | 中国食品药品检定研究院 | Comprehensive evaluation method for quality of traditional Chinese medicinal materials |
| CN113720931A (en) * | 2021-08-19 | 2021-11-30 | 遂昌县森林王中药材专业合作社 | Quality evaluation method of radix tetrastigme medicinal material |
| CN113720931B (en) * | 2021-08-19 | 2023-10-20 | 遂昌县森林王中药材专业合作社 | Quality evaluation method of radix tetrastigme medicinal material |
| CN115128226A (en) * | 2022-07-19 | 2022-09-30 | 四川农业大学 | Sichuan tangerine peel quality determination method and planting method based on producing area and effective components |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102590462A (en) | Method for evaluating comprehensive quality of medicinal material Polygonum capitatum of Relinqing granules | |
| Liu et al. | Influence of environmental factors on the active substance production and antioxidant activity in Potentilla fruticosa L. and its quality assessment | |
| Melito et al. | Altitude and climate influence Helichrysum italicum subsp. microphyllum essential oils composition | |
| Liu et al. | Coordinated variation in leaf and root traits across multiple spatial scales in Chinese semi‐arid and arid ecosystems | |
| Peng et al. | Fingerprint profile of active components for Artemisia selengensis Turcz by HPLC–PAD combined with chemometrics | |
| Monteiro et al. | Dietary antioxidants in coffee leaves: Impact of botanical origin and maturity on chlorogenic acids and xanthones | |
| He et al. | Authentication of the geographical origin of Maca (Lepidium meyenii Walp.) at different regional scales using the stable isotope ratio and mineral elemental fingerprints | |
| Ding et al. | A rapid integrated bioactivity evaluation system based on near-infrared spectroscopy for quality control of Flos Chrysanthemi | |
| Zhao et al. | Plant water use strategy in response to spatial and temporal variation in precipitation patterns in China: A stable isotope analysis | |
| Harnly et al. | Comparison of flow injection MS, NMR, and DNA sequencing: methods for identification and authentication of black cohosh (Actaea racemosa) | |
| Pei et al. | Application of authentication evaluation techniques of ethnobotanical medicinal plant genus Paris: a review | |
| Liu et al. | Study on Quality Response to Environmental Factors and Geographical Traceability of Wild Gentiana rigescen s Franch | |
| Wang et al. | Geographic authentication of Eucommia ulmoides leaves using multivariate analysis and preliminary study on the compositional response to environment | |
| Souhila et al. | Identification and quantification of phenolic compounds of Artemisia herba-alba at three harvest time by HPLC–ESI–Q-TOF–MS | |
| Vendruscolo et al. | Monitoring desertification in semiarid Brazil: using the Desertification Degree Index (DDI) | |
| Yu et al. | Effect of genotype and environment on five bioactive components of cultivated licorice (Glycyrrhiza uralensis) populations in northern China | |
| Li et al. | Characteristic fingerprint based on low polar constituents for discrimination of Wolfiporia extensa according to geographical origin using UV spectroscopy and chemometrics methods | |
| Singh et al. | Gas chromatography–mass spectrometry (GC–MS) profiling reveals substantial metabolome diversity in seabuckthorn (Hippophae rhamnoides L.) berries originating from different geographical regions in the Indian Himalayas | |
| Kuiawski et al. | Vegetation indexes and delineation of management zones for soybean | |
| Misra et al. | Chemodiversity and molecular variability in the natural populations (India) of Gloriosa superba (L.) and correlation with eco-geographical factors for the identification of elite chemotype (s) | |
| Luo et al. | HPLC-based metabolomics of Dendrobium officinale revealing its antioxidant ability | |
| Wei et al. | Evaluation of two parts of Lithocarpus polystachyus Rehd. from different Chinese areas by multicomponent content determination and pattern recognition | |
| Moosmang et al. | Differentiation between cistus l.(sub-) species (cistaceae) using nmr metabolic fingerprinting | |
| Liu et al. | Chemical fingerprinting of wild germplasm resource of Ophiopogon japonicus from Sichuan basin, China by RP-HPLC coupled with hierarchical cluster analysis | |
| Prada-Muñoz et al. | Targeted Anthocyanin Profiling of Fruits from Three Southern Highbush Blueberry Cultivars Propagated in Colombia |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20120718 |