CN102590067B - Loading device capable of utilizing common microscope to conduct blood cell phase imaging - Google Patents
Loading device capable of utilizing common microscope to conduct blood cell phase imaging Download PDFInfo
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- CN102590067B CN102590067B CN201210028420.1A CN201210028420A CN102590067B CN 102590067 B CN102590067 B CN 102590067B CN 201210028420 A CN201210028420 A CN 201210028420A CN 102590067 B CN102590067 B CN 102590067B
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Abstract
The invention relates to a microscopic imaging device of biological cell phase, in particular to a loading device capable of utilizing a common microscope to conduct blood cell phase imaging. The loading device comprises a laser, a first prism with coating film faces as a first light distribution face and a first reflection face respectively, a second prism with coating film faces as a second light distribution face and a second reflection face respectively and a prism installation frame body with an objective table. The loading device can be installed on a two-dimensional adjustable objective table of the common microscope, and a microscopic phase image is formed by applying the M-Z interference principle and combining and interfering light beams passing through blood cells and light beams not passing through the blood cells. The image is amplified to form an image in microscopic mode through the loaded common microscope, and the image formed in the microscopic amplifying mode can be connected with a computer to conduct phase unpacking and be inverted to form a three-dimensional cell image. The loading device resolves the imaging problem that the common microscope cannot conduct microscopy on blood cell transparent bodies.
Description
Technical field
The present invention relates to a kind of biological cell phase place micro-imaging equipment, refer in particular to a kind of charger that utilizes simple microscope can carry out haemocyte phase imaging.
Background technology
The form detection and classification of haemocyte has important application in fields such as life science, clinical medicine, bioengineering, blood cell shape detection technique field is the science and technology that technical field more than is intersected, and wherein optics microscopy is a main method and technology, haemocyte is due to its optical transparence, therefore in microscopy, generally all needs dyeing, and the destruction that dyeing easily causes cell ortho states, so phase place is micro-, becomes a kind of up-to-date, the most useful method, phase place micro-imaging is mainly that application interference of light principle produces phase image, and then unpack the 3-D view of reconstruct sample, there is at present the patent application technology that some microscopes are relevant with phase microscope, for example patented technology CN97108464.5 (false-colour optical stereomicroscope device and method thereof) utilizes spot light, microscope machinery cylinder is transformed, pack color separation grating into, Fourier transformation lens and four-dimensional adjustable rectangular filter, form a kind of white light picture information disposal system of distortion, thereby on microscope, realized object microstructure by the direct observation of the colored stereo image of vacation of oblique illumination black and white gray-level difference and phase change rate modulation, but this invention technology can not be to transparent haemocyte micro-imaging, patented technology CN2009 and for example
The method of a 10086592.2(quantitative digital microscopic phase contrast imaging) it ordinary numbers optical microscope platform and ordinary individual with realizing on computing machine, this technology gathers experiment view data on focal plane by the mode of manual or automatic focusing, control objective table moves, on upper and lower wrong focal plane, gather respectively experiment view data, two width images on focal plane epigraph based on above-mentioned collection and upper and lower wrong focal plane, calculating strength is at the rate of change of optical axis direction, utilize technology to solve light intensity propagation equation, realizing quantitative phase recovers and microscopic phase contrast imaging, but this invention technology is only from microscope light path, not invent, but a kind of invention on algorithm, also just like foreign patent technology CN101727059 (A) (Digital holographic micro scopic imaging method based on surface plasma resonance and microscope based on surface plasma body resonant vibration and micro-under digital holographic microscopic imaging) the method is mainly a kind of phase place based under two-beam interference method and the holographic microphotography method of intensity, and for example this technology of foreign patent technology US2009290156 (A1) (the Fourier transform light scattering transmission method of Spatial light interference microscopy and fourier transform light scattering for cell and tissue characterization spatial light interference microscope and cell tissue) is mainly a kind of phase of cells formation method based under Fourier scattering method, these two kinds of external patented technologies, have good advance and professional, but instrument cost is high, purposes is single and application threshold is high, the present invention is the micro-imaging function based on simple microscope, a kind of stable performance proposing according to interference of light principle, charger patented technology simple in structure, with low cost, simple to operate, easy for installation, this device patented technology not only can keep the function of original simple microscope but also have the function that has increased phase of cells imaging.
Summary of the invention
The object of patent of the present invention is to provide a kind of charger that is carried in the imaging of the enterprising promoting circulation of blood phase of cells of simple microscope.
The technology of the present invention solution is:
A kind of charger that utilizes simple microscope can carry out haemocyte phase imaging, described charger is fixed on the adjustable objective table of microscope, it is characterized in that: described charger comprise the first prism, coated surface that laser instrument, coated surface are respectively the first light splitting surface and the first reflecting surface be respectively the second prism of the second reflecting surface and the second light splitting surface, with the prism installation frame body of the micro-objective table of phase place; The horizontal axis of the first prism is arranged on prism installation frame body bottom with the coaxial also level of laser instrument axle, and the first light splitting surface of the first prism is positioned at the first reflecting surface of the first prism and the centre of laser instrument; The second prism coaxially stacks with the first prism is vertical, be arranged on prism installation frame body top, the second reflecting surface of the second prism is coaxial in the vertical direction with the first light splitting surface of the first prism, and the second light splitting surface of the second prism is coaxial in the vertical direction with the first reflecting surface of the first prism; Light hole is placed in prism installation frame body topmost, coaxial with the second light splitting surface of the second prism and the first reflecting surface of the first prism; The micro-objective table of phase place and prism installation frame body one and be positioned at the second light splitting surface of the second prism and the centre of the first reflecting surface of the first prism; There is a dismountable cover plate in the left side of support body, fixes silk hole be fixed in prism installation frame body by cover plate.
Laser light source sends the light beam of horizontal direction, the first light splitting surface transmitting forward by the first prism is divided into the two-beam that continues horizontal transport and transmit vertically upward, the first reflecting surface changed course that the light beam forward direction of continuation horizontal transport runs into the first prism is light beam vertically upward, this light beam becomes the second light splitting surface that object light enters the second prism after being placed on the cell sample on the micro-objective table of phase place, is directly transferred to the object lens of microscopic system; By first prism the first light splitting surface, be divided into light beam that continuation transmits vertically upward vertically to the second reflecting surface of the second prism, the beam Propagation that changes horizontal direction after reflection into causes the second light splitting surface of the second prism, after reflection, become reference light changed course for vertical direction, enter coaxially, in the same way the object lens of microscopic system with object light; The micro-amplification system that object light and reference light consist of micro objective, eyepiece interferences of meeting, the phase image of formation cell sample; Whole device is arranged on the adjustable objective table of microscopical bidimensional, by horizontal location, can obtain phase of cells image clearly with after vertical focusing.
Described prism is made with quartz glass, and reflecting surface is the light beam face of meeting of plated film fully reflecting surface; Light splitting surface is the light beam face of meeting of plated film half reflection, half transmitting, is respectively 50% of incident intensity; Reflecting surface is strictly parallel with light splitting surface, and each and 45 °, the prism installation frame body bottom surface angle of cut are guaranteed the effect of M-Z optical interference circuit.
Described simple microscope should configure the adjustable objective table of bidimensional, as not configuration, can buy finished product; When apparatus of the present invention are arranged on its platform, can carry out plane bidimensional fine setting location, guarantee that object light and reference light and microscopes optical axis are coaxial, to realize better target of the present invention.
When simple microscope carries out general micro-amplification imaging, the beam orifice surface that its sample only need to be placed on apparatus of the present invention is that common micro-objective table can be realized general microscope functions; When needs phase place is micro-, cell sample can be placed to the micro-objective table of the phase place place of this device, can realize the phase place microscopic function of cell.
Patent of the present invention is compared its significant advantage with correlation technique: 1, on simple microscope, load apparatus of the present invention and can realize the micro-patented technology of phase place and there is not yet report, belong to original; 2, the feature of the conventional light path of M-Z has guaranteed high reliability and the high stability of apparatus of the present invention; 3, biprism structure has shown the convenience of integrally-built simplicity and operation debugging; 4, the loading method of apparatus of the present invention, had both guaranteed original microscopical function, had increased again the micro-function of phase place, had realized a machine difunctional; Therefore, instrument cost is low 5, apparatus of the present invention have effectively utilized former microscopical micro-amplification, digital imagery, longitudinally parts and the function thereof of focusing and plane bidimensional location, and, application surface is broad.
Accompanying drawing explanation
Fig. 1 is the structural representation that a kind of simple microscope of the present invention changes the micro-dual purpose charger of phase of cells into;
Fig. 2 is that a kind of simple microscope of the present invention changes the micro-dual purpose M-Z interference of light principle schematic of phase of cells into;
1, laser instrument 2, the first light splitting surface 3 of the first prism, the second reflecting surface 4 of the second prism, the second light splitting surface 5 of the second prism, the first reflecting surface 6 of the first prism, light hole 7, prism location adjustment column 8, prism installation frame body 9, the fixing silk of cover plate hole 10, laser instrument positioning filament hole 11, the fixing silk of support body hole 12, the micro-objective table 13 of phase place, microscope is adjustable objective table 14, common micro-objective table 15, the microscopic system 16 of simple microscope, cell sample.
Embodiment
In conjunction with Fig. 1, Fig. 2, it is a M-Z light path interference system that a kind of simple microscope of the present invention changes the micro-dual purpose charger of phase of cells into, includes laser instrument 1, plated film becomes the first light splitting surface 2 and the first prism of the first reflecting surface 5 respectively, plated film becomes the micro-objective table 12 of the second prism, phase place, beam orifice 6 and the support body 8 of the second reflecting surface 3 and the second light splitting surface 4 to form respectively.
The collimated light beam that laser instrument 1 sends through the first prism the first light splitting surface 2 be divided into that level is advanced and the two-beam of light beam of transmission vertically upward, the first reflecting surface 5 changed courses that the light beam forward direction of continuation horizontal transport runs into the first prism are light beam vertically upward, this light beam becomes the second light splitting surface 4 that object light enters the second prism after by cell sample 16, is directly transferred to simple microscope system 15; The light beam that is divided into transmission vertically upward by the first light splitting surface 2 of the first prism is vertically to the second reflecting surface 3 of the second prism, the beam Propagation that changes horizontal direction after reflection into causes the second light splitting surface 4 of the second prism, after reflection, become reference light changed course for vertical direction, enter coaxially, in the same way simple microscope system 15 with object light; Object light and reference light, by microscopic system 15 interference of meeting, form the phase image of cell sample; Adjustment column 7 corrective actions of prism location guarantee each reflecting surface and light splitting surface parallel to each other; In laser instrument positioning filament hole 10, laser instrument is installed, coaxial for guaranteeing the first reflecting surface 5 centers of the optical axis of laser instrument 1 and the first light splitting surface 2 of the first prism, the first prism; The fixing silk of cover plate hole 9 is fixing for support body cover plate; Whole charger is fixed silk hole 11 by support body and is arranged on the adjustable objective table 13 of microscopical bidimensional, the coherent light beam that regulates the adjustable objective table 13 of bidimensional to adjust this device light hole 6 is coaxial with microscopes optical axis, then by obtaining phase of cells image clearly after the vertical focusing of simple microscope system 15; The micro-objective table 12 of phase place and common micro-objective table 14 be for the loading of phase place and common two kinds of micro-patterns of apparatus of the present invention, thereby finally realize object of the present invention.
Claims (2)
1. a charger that utilizes simple microscope can carry out haemocyte phase imaging, described charger is fixed on the adjustable objective table of microscope, it is characterized in that: described charger comprise the first prism, coated surface that laser instrument, coated surface are respectively the first light splitting surface and the first reflecting surface be respectively the second prism of the second reflecting surface and the second light splitting surface, with the prism installation frame body of the micro-objective table of phase place; The horizontal axis of the first prism is arranged on prism installation frame body bottom with the coaxial also level of laser instrument axle, and the first light splitting surface of the first prism is positioned at the first reflecting surface of the first prism and the centre of laser instrument; The second prism coaxially stacks with the first prism is vertical, be arranged on prism installation frame body top, the second reflecting surface of the second prism is coaxial in the vertical direction with the first light splitting surface of the first prism, and the second light splitting surface of the second prism is coaxial in the vertical direction with the first reflecting surface of the first prism; Light hole is placed in prism installation frame body topmost, coaxial with the second light splitting surface of the second prism and the first reflecting surface of the first prism; The micro-objective table of phase place and prism installation frame body one and be positioned at the second light splitting surface of the second prism and the centre of the first reflecting surface of the first prism; There is a dismountable cover plate in the left side of prism installation frame body, fixes silk hole be fixed in prism installation frame body by cover plate.
2. a kind of charger that utilizes simple microscope can carry out haemocyte phase imaging as claimed in claim 1, is characterized in that: described prism is made with quartz glass, and reflecting surface is the light beam face of meeting of plated film fully reflecting surface; Light splitting surface is the light beam face of meeting of plated film half reflection, half transmitting, is respectively 50% of incident intensity; Reflecting surface is strictly parallel with light splitting surface, each and 45 °, the prism installation frame body bottom surface angle of cut.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210028420.1A CN102590067B (en) | 2012-02-09 | 2012-02-09 | Loading device capable of utilizing common microscope to conduct blood cell phase imaging |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210028420.1A CN102590067B (en) | 2012-02-09 | 2012-02-09 | Loading device capable of utilizing common microscope to conduct blood cell phase imaging |
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| CN102590067A CN102590067A (en) | 2012-07-18 |
| CN102590067B true CN102590067B (en) | 2014-02-12 |
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| CN201210028420.1A Expired - Fee Related CN102590067B (en) | 2012-02-09 | 2012-02-09 | Loading device capable of utilizing common microscope to conduct blood cell phase imaging |
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| EP3575773A1 (en) * | 2018-05-28 | 2019-12-04 | Universität für Bodenkultur Wien | A method for determining a three-dimensional particle distribution in a medium |
| CN111929298B (en) * | 2020-07-20 | 2023-04-07 | 广东工业大学 | Observation method for simultaneous focusing of microfluidic channels in y and z directions under microscope |
Citations (6)
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|---|---|---|---|---|
| CN85105355A (en) * | 1985-07-11 | 1987-01-14 | 中国科学院上海光学精密机械研究所 | Differential interference phase-contrast microscope |
| WO2005001445A3 (en) * | 2001-12-18 | 2005-12-15 | Massachusetts Inst Technology | Systems and methods for phase measurements |
| CN101346673A (en) * | 2005-12-22 | 2009-01-14 | 相位全息成像Phi有限公司 | Method and apparatus for analysis of a sample of cells |
| CN101727059A (en) * | 2009-12-22 | 2010-06-09 | 暨南大学 | Digital holographic microscopic imaging method based on surface plasma resonance and microscope |
| CN102207613A (en) * | 2011-06-03 | 2011-10-05 | 北京工业大学 | Point source reference optical distortion compensation digital holography phase contrast microscope |
| JP2011253123A (en) * | 2010-06-03 | 2011-12-15 | Olympus Corp | Microscope |
-
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- 2012-02-09 CN CN201210028420.1A patent/CN102590067B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85105355A (en) * | 1985-07-11 | 1987-01-14 | 中国科学院上海光学精密机械研究所 | Differential interference phase-contrast microscope |
| WO2005001445A3 (en) * | 2001-12-18 | 2005-12-15 | Massachusetts Inst Technology | Systems and methods for phase measurements |
| CN101346673A (en) * | 2005-12-22 | 2009-01-14 | 相位全息成像Phi有限公司 | Method and apparatus for analysis of a sample of cells |
| CN101727059A (en) * | 2009-12-22 | 2010-06-09 | 暨南大学 | Digital holographic microscopic imaging method based on surface plasma resonance and microscope |
| JP2011253123A (en) * | 2010-06-03 | 2011-12-15 | Olympus Corp | Microscope |
| CN102207613A (en) * | 2011-06-03 | 2011-10-05 | 北京工业大学 | Point source reference optical distortion compensation digital holography phase contrast microscope |
Non-Patent Citations (2)
| Title |
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| 刘晓元 等.普通显微镜改装成相衬显微镜的一种新方法.《光子学报》.1999,第28卷(第1期), |
| 普通显微镜改装成相衬显微镜的一种新方法;刘晓元 等;《光子学报》;19990131;第28卷(第1期);80-82 * |
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Application publication date: 20120718 Assignee: Zhenjiang fine Kant Photoelectric Technology Co., Ltd. Assignor: Jiangsu University Contract record no.: 2015320000634 Denomination of invention: Loading device capable of utilizing common microscope to conduct blood cell phase imaging Granted publication date: 20140212 License type: Exclusive License Record date: 20151117 |
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