CN102586101B - Industrial microalgae magnetic separation and collection device - Google Patents
Industrial microalgae magnetic separation and collection device Download PDFInfo
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Abstract
The invention relates to an industrial microalgae magnetic separation and collection device, which comprises a mixing system, a magnetic separation system, an algae collection system, a press filtration system, a synthesis stirring tank and a magnetic particle solution storage system in sequential connection, wherein the magnetic particle solution storage system is connected with the mixing system. The complete set of the microalgae magnetic separation device provided by the invention can be used for continuous separation and collection of microalgae cells, realizes recovery, regeneration and repeated use of magnetic particles, reduces the cost of microalgae magnetic separation, solves the problem of reclaimed water reuse during microalgae culture process, is favorable for industrial scale-up, and is suitable for large-scale industrial production.
Description
Technical field
The present invention relates to a kind of little algae separated and collected device, specially refer to a kind of industrialization with little algae magnetic resolution collection device.
Background technology
Little algae is little, simple in structure, the unicellular algae rapidly that grows of volume, and its distribution is extremely extensive, until the two poles of the earth, north and south, to seawater, to ocean depths nearly all has little algae exist and procreation from water meter from freshwater lake, saline and alkaline marsh to the frigid zone from tropical, temperate zone.Many little algaes have important economic value.For a long time, people only are the direct or indirect living bait of little algae as fish, shrimp, the shellfish young or adult.In recent decades, especially after the eighties in 20th century, little algae biotechnology develops rapidly, people recognize the polyphyly of little algae on evolving, the diversity of heredity, contain multiple special secondary metabolites in the frustule, little in addition algae also have transform light energy rate height, strong to environmental compatibility, be easy to characteristics such as genetic improvement, can carry out large scale culturing to it.At present, the using value of little algae in fields such as food, medicine, chemical industry, environmental protection, the energy become the focus that people pay close attention to, and little algae is cultivated and the related products exploitation becomes the emerging technology industry.
Separation of little algae and collection become the little algae of restriction and cultivate one of principal element of scale operation.The separation of present little algae and the method for collection mainly comprise: centrifugal, filtration, flocculation, air supporting.Adopt centrifugal method to need expensive fixed capital (whizzer) investment and energy consumption big; Adopt the efficient of filter method low, film stops up easily, and operation easier is big; The employing flucculation process separates and collects little algae and need use various flocculation agents, and flocculation agent and little algae separation difficulty cause the pollution of target product easily; Air supporting/bubble carries to be separated in needs the auxiliary of tensio-active agent usually when using in little algae, exist the problem same with flucculation process.
In order to improve defective or the deficiency that traditional little algae separation method exists, the research and development personnel have designed different little algae tripping devices.CN 101457201B discloses a kind of lining rod type bubble separation apparatus, this device reduces the foam liquid holdup by equally distributed many lining bars of fixed on the blind flange, the concentration of the microalgae cell that improve to separate obtains, but this improvement and failing solve bubble carry separate in the pollution problem of tensio-active agent; CN101693878A discloses the method and apparatus that a kind of little algae concentrates and collects, its core is that the employing micro-strainer filters the moisture in little algae and holds back little algae, recoil by solenoid control, realize concentrating and operate continuously of little algae, traditional backwash method that this equipment adopts solves the problem of film gambling plug, difficulty is big, and poor effect only plays certain inspissated to micro algae culturing liquid; CN 102127509A discloses a kind of method and apparatus that separates little algae, and the essence of this separation method is the auxiliary dissolved air flotation of tensio-active agent, is faced with the pollution problem of tensio-active agent equally; CN 20202555U discloses a kind of BFM membrane sepn and has removed the phytem system, this patent just combines microporous filter with crooked board-like film (BFM) separation system, when removing algae, increase the clearance of nitrogen phosphorus and COD, and fail to propose solution preferably for the key problem that the film that exists in little algae membrane sepn stops up; CN 102234613A and CN 101811757A all disclose the little algae separation method of the auxiliary electricity flocculation of a kind of air supporting, designed the staggered oscillating screen plate tower of positive and negative electrode respectively and realized separating of little algae with the staggered concentric drums battery lead plate of positive and negative electrode, but electric flocculation process is because the anodic dissolving can bring the pollution problem of flocculation agent to microalgae cell equally.In sum, though the research staff has obtained some progress for the development of little algae tripping device and exploitation, the key problem that exists in the little algae separation method of still unresolved tradition can not satisfy the demand of suitability for industrialized production.
Because magneticsubstance has the separation of being easy to, lock out operation characteristic of simple, in recent years, adopted magneticsubstance to carry out the existing report of the isolating method of little algae.(Gao Z.W. such as Gao and Peng, Peng X.J., ZhangH.M., Luan Z.K., Fan B.Desalination, 2009,247,337) mixture of employing montmorillonite and copper ferriferous oxide successfully separates harmful little algae microcystic aeruginosa (Microcystis aeruginosa) from water, its magnetic resolution medium can be reused, but the synthetic comparatively loaded down with trivial details and large usage quantity of this magnetic composite; (Liu D. such as Liu and Li, Li F.T., Zhang B.R.Water Sci.Technol.2009,59,1085) adopt chitosan-modified magnetic polymer as the magnetic resolution medium, successfully remove the algal tufa in the fresh water, but its magnetic resolution medium is synthetic comparatively complicated, the magnetic resolution medium can't be reused, and is subjected to ionic strength affect bigger.For the cost that reduces little algae magnetic resolution, the recovery that realizes the magnetic resolution medium, Xu and Guo adopt exposed magnetic Nano Fe
3O
4Particle carries out the magnetic resolution of microalgae cell, and has realized recovery (Ling Xu, the Chen Guo of magnetic resolution medium by the molten method of acid, Feng Wang, Sen Zheng, Chun-Zhao Liu.Bioresource Technology 2011,102,10047).This shows that little algae magnetic resolution is feasible on technology and method.Realize the application of magnetic resolution in little algae suitability for industrialized production, one of its key issue provides complete little algae magnetic resolution equipment, and little algae magnetic resolution is a brand-new field, does not still have the report and the business-like product of relevant little algae magnetic resolution equipment at present.
Summary of the invention
The present invention is directed to a series of problems that existing little algae isolation technique exists, special in to satisfy the demand of little algae magnetic resolution on equipment, provide a kind of industrialization with little algae magnetic resolution collection device.
For reaching this purpose, the present invention by the following technical solutions:
A kind of industrialization comprises the mixing system, magnetic resolution system, frond gathering system, press filteration system, magnetic-particle synthesis system and the magnetic-particle solution stocking system that connect successively with little algae magnetic resolution collection device; Described magnetic-particle solution stocking system is connected with mixing system.
Mixing system of the present invention links to each other with reactor by first feed pump; Magnetic-particle solution stocking system links to each other with mixing system by second feed pump.The magnetic resolution system links to each other with reactor by the 3rd feed pump; Be provided with liquid meter between magnetic resolution system and mixing system.
Liquid volume keeps constant in the mixing system, and the residence time of pumping liquid is determined by processing condition.Liquid meter is used for controlling the discharge of mixing system, makes the liquid volume in the mixing system keep constant.Mixing system is provided with whipping appts, little algae is contacted better and by the combination of charge effect power with magnetic-particle.
Described frond gathering system is provided with whipping appts, and magnetic-particle dissolves fully when guaranteeing to add the acid dissolving, and its quantity is more than 2 or 2, connects with parallel form.When polymer volume in one of them jar reach the tank body volume 60% the time, switch to another jar and proceed frond and collect, the jar that frond is housed then carries out follow-up magnetic-particle and reclaims technology.
Described press filteration system is provided with tensimeter, compressed air inlet, and its inside center position is provided with spray thrower, and the spray washing frond remains in magnetic-particle lysate in the frond with removal in filtration procedure.
The magnetic-particle synthesis system is provided with temperature regulating device, to satisfy the temperature requirements of magnetic-particle in synthetic.
Described magnetic-particle solution stocking system is provided with whipping appts, the generation of the solids precipitation phenomenon of avoiding magnetic-particle solution to leave standstill for a long time causing.
Described frond holding tank, pressure-filtering tank and synthetic stirred pot all adopt stainless material to make.
Magnetic resolution of the present invention system comprises separator tank, rotary head drum, scraper plate, guide channel and spray equipment group for the rotation magnetic separating device.
The axis of described rotary head drum is vertical with fluidic flow direction in the separator tank.The outer wall of scraper plate and rotary head drum is tangent, can be scraped in the guide channel fully with the mixture that guarantees microalgae cell and magnetic-particle.Guide channel closely is connected with scraper plate, and becomes 5-30 ° of angle with horizontal plane, and preferred 15 ° of angles can make the polymkeric substance of microalgae cell and magnetic-particle can rely on gravity to import in the frond holding tank.
Described rotary head drum comprises concentric fixedly inner core and rotation urceolus, and inner core maintains static, and urceolus can rotate freely around the magnetic drum axis.Fixedly the inner core lower surface is provided with bar permanent magnet, is arranged in parallel with the rotary head drum axis; Rotation urceolus lower edge to the distance of separator tank bottom is 1-3cm, 1.2cm for example, 1.5cm, 2.1cm, 2.6cm, 2.9cm etc., preferred 2cm.
Described bar permanent magnet is relative with scraper plate, and coverage is in 90 ° of scopes of axial plane below one side in the rotary head drum; Magneticstrength is 2000-5000Gs, 2010Gs for example, 2600Gs, 2950Gs, 3420Gs, 3800Gs, 4360Gs, 4750Gs, 4980Gs etc., further preferred 3500Gs.
The fixedly inner core of described rotary head drum adopts technically pure iron or soft steel; The rotation urceolus adopts non-magnetic stainless steel, and its sense of rotation is opposite with fluid flow direction.
Liquid levels is no more than place, rotary head drum axis horizontal plane in the described separator tank.
Described spray equipment group be positioned at be parallel to the scraper plate upper edge directly over, the coverage of spray equipment group is scraper plate place one side, can not surpass the urceolus upper edge, its objective is the surging force and a spot of water that produce by spray, the polymkeric substance that prevents microalgae cell and magnetic-particle adheres to the obstruction that guide channel is caused on scraper plate or guide channel surface.
The switch of spray equipment group, frequency of utilization and power by separation microalgae cell and magnetic-particle form the decision of polymeric adhesion characteristics.
Described magnetic-particle is exposed nanometer Fe
3O
4Particle.When the size of particle is reduced to nanometer scale, because the influence of small-size effect, surface effects, quantum size effect and the macro quanta tunnel effect etc. of nanoparticle makes its local be different from the special magnetic property of conventional body phase material.Fill alkaline solution in the magnetic-particle synthesis system, be preferably NaOH solution or NH
3H
2O solution.
Little algae magnetic resolution collection device provided by the invention, by first feed pump little algae in algae pond or the reactor is pumped in the mixing system, simultaneously an amount of magnetic-particle solution is pumped in the mixing system and the microalgae cell polymerization, then mixed solution carries out magnetic resolution through liquid meter inflow rotary magnetic tripping device, enter the frond holding tank after the polymkeric substance that microalgae cell and magnetic-particle form is separated, clear liquid reenters in algae pond or the reactor through the 3rd feed pump.After adding hydrochloric acid and stirring make the magnetic-particle dissolving in the frond holding tank, change in the press filteration system, the clear liquid that obtains after the press filtration changes in the magnetic-particle synthesis system and adds an amount of alkaline solution, the magnetic-particle solution that reaction obtains transfers pH to change magnetic-particle solution storage tank over to neutrality, is used for the separation of little algae.Device provided by the invention can be realized the reuse of water in little algae separated and collected process and the recovery of magnetic-particle.
Compare with the prior art scheme, beneficial effect of the present invention is:
1, according to the characteristics of little algae magnetic resolution, adopt complete little algae magnetic resolution device, realized recovery, regeneration and the repeated use of magnetic-particle, reduced the cost of little algae magnetic resolution;
2, adopt the rotary magnetic tripping device, the polymkeric substance that microalgae cell and magnetic-particle can be formed from mixed solution, separating and collect rapidly and efficiently separate the clear liquid that obtains and do not contain any impurity, solved the reuse problem of water in little algae culturing process;
3, adopt the filtering equipment that has installed spray thrower additional, the iron ion that remains in the algae mud can be washed out to greatest extent, enter in the filtrate, both reduced the pollution of iron ion, improved the rate of recovery of magnetic-particle again microalgae cell;
4, complete little algae magnetic resolution device provided by the invention can carry out the continuous separated and collected of microalgae cell, helps industry and amplifies, and is applicable to large-scale commercial production.
Description of drawings
Fig. 1 is the structural representation of the specific embodiment of the invention;
Fig. 2 is the vertical view of rotary magnetic tripping device;
Fig. 3 is the front view of rotary magnetic tripping device;
Fig. 4 is the side-view of rotary magnetic tripping device.
Reference numeral is expressed as follows respectively:
1, reactor 2-1, the first feed pump 2-2, second feed pump
2-3, the 3rd feed pump 3, mixing system 4, rotary magnetic tripping device
5, liquid meter 6, magnetic-particle solution stocking system
7, frond gathering system 8, tensimeter 9, press filteration system
10, compressed air inlet 11, spray thrower 12, magnetic-particle synthesis system
13, guide channel 14, scraper plate 15, rotary head drum
16, fixedly inner core 17, bar permanent magnet 18, rotation urceolus
19, separator tank 20, spray equipment group
Below the present invention is described in more detail.But following example only is a simple and easy example of the present invention, does not represent or limit the scope of the present invention, and interest field of the present invention is as the criterion with claims.
Embodiment
For the present invention is described better, be convenient to understand technical scheme of the present invention, typical but non-limiting embodiment of the present invention is as follows:
A kind of industrialization comprises the mixing system 3, magnetic resolution system, frond gathering system 7, press filteration system 9, magnetic-particle synthesis system 12 and the magnetic-particle solution stocking system 6 that connect successively with little algae magnetic resolution collection device; Described magnetic-particle solution stocking system 6 is connected with mixing system 3.
Mixing system 3 links to each other with reactor 1 by the first feed pump 2-1; Described magnetic-particle solution stocking system 6 links to each other with mixing system 3 by the second feed pump 2-2.The magnetic resolution system links to each other with reactor 1 by the 3rd feed pump 2-3; 3 of magnetic resolution system and mixing systems are provided with liquid meter 5.
Mixing system 3, frond gathering system 7 and magnetic-particle solution stocking system 6 are equipped with whipping appts.Press filteration system 9 is provided with tensimeter 8, compressed air inlet 10, and its inside center position is provided with spray thrower 11.Magnetic-particle synthesis system 12 is provided with temperature regulating device.
The concrete structure of magnetic resolution system is as follows: the magnetic resolution system comprises separator tank 19, rotary head drum 15, scraper plate 14, guide channel 13 and spray equipment group 20 for rotation magnetic separating device 4; The axis of described rotary head drum 15 is vertical with fluidic flow direction in the separator tank 19; Scraper plate 14 is tangent with the outer wall of rotary head drum 15; Guide channel 13 closely is connected with scraper plate 14, and becomes 5-30 ° of angle with horizontal plane, is preferably 15 ° of angles; Spray equipment group 20 is parallel be located at scraper plate 14 directly over, it highly is lower than rotary head drum 15 upper edges.
Bar permanent magnet 17 is relative with scraper plate 14, and coverage is in 90 ° of scopes of axial plane below one side in the rotary head drum 15; Magneticstrength is 2000-5000Gs, is preferably 3500Gs.
The fixedly inner core 16 of rotary head drum 15 adopts technically pure iron or soft steel; Rotation urceolus 18 adopts non-magnetic stainless steel, and its sense of rotation is opposite with fluid flow direction.
Liquid levels is no more than place, rotary head drum 15 axis horizontal plane in the separator tank 19.
Specific operation process is: the first feed pump 2-1 pumps into the microalgae cell nutrient solution in the reactor 1 in the mixing system 3, the second feed pump 2-2 will pump in the mixing system 3 by an amount of magnetic-particle solution of processing compound and mix with microalgae cell simultaneously, and after carry out magnetic resolution in the liquid meter 5 inflow rotary magnetic tripping devices 4, the polymkeric substance that microalgae cell and magnetic-particle form with change frond holding tank 7 over to after mixed solution separates, clear liquid reenters the cultivation that is used for little algae in the reactor 1 through the 3rd feed pump 2-3, in the frond holding tank, add hydrochloric acid and stir the magnetic-particle dissolving that makes in the polymkeric substance, reaction changes in the press filteration system 9 after finishing, the clear liquid that obtains after the press filtration changes in the magnetic-particle synthesis system 12 and adds an amount of alkaline solution, and the magnetic-particle solution that reaction obtains transfers pH to change the separation that is used for little algae in the magnetic-particle solution stocking system 6 over to neutrality.
Embodiment one: the separated and collected of frond in Blang's grape algae culturing liquid
In device of the present invention, carry out the separated and collected of frond in Blang's grape algae culturing liquid; Blang's grape algae culturing liquid enters mixing system by first feed pump; after the magnetic-particle that pumps into simultaneously mixes; enter and carry out magnetic resolution in the rotary head drum tripping device; the polymkeric substance of frond cell and magnetic-particle enters the frond holding tank; in continuous separated and collected process in purified liquor outlet sampling and measuring clear liquid micro algae biomass, the frond rate of recovery of grape algae is 98.5%.When treating that polymer volume in the frond holding tank reaches set(ting)value, switch frond holding tank 7, adding hydrochloric acid in the holding tank of collecting frond dissolves magnetic-particle, and after press filtration and regeneration, in the concentration of magnetic-particle synthesis system outlet sampling and measuring regeneration magnetic-particle, the rate of recovery of magnetic-particle reaches 95.4%.
Embodiment two: the separated and collected of frond in the chlorella nutrient solution
In device of the present invention, carry out the separated and collected of frond in the chlorella nutrient solution; the chlorella nutrient solution enters mixing system by first feed pump; after the magnetic-particle that pumps into simultaneously mixes; enter and carry out magnetic resolution in the rotary head drum tripping device; the polymkeric substance of frond cell and magnetic-particle enters the frond holding tank; in continuous separated and collected process in purified liquor outlet sampling and measuring clear liquid micro algae biomass, the frond rate of recovery of chlorella is 98.9%.When treating that polymer volume in the frond holding tank reaches set(ting)value, switch the frond holding tank, adding hydrochloric acid in the holding tank of collecting frond dissolves magnetic-particle, and after press filtration and regeneration, in the concentration of magnetic-particle synthesis system outlet sampling and measuring regeneration magnetic-particle, the rate of recovery of magnetic-particle reaches 95.6%.
Applicant's statement, the present invention illustrates detailed structure feature of the present invention and separated and collected method by the foregoing description, but the present invention is not limited to above-mentioned detailed structure feature and separated and collected method, does not mean that promptly the present invention must rely on above-mentioned detailed structure feature and the separated and collected method could be implemented.The person of ordinary skill in the field should understand, any improvement in the present invention to the increase of the equivalence replacement of the selected parts of the present invention and accessory, the selection of concrete mode etc., all drops within protection scope of the present invention and the open scope.
Claims (10)
1. an industrialization comprises the mixing system (3), magnetic resolution system, frond gathering system (7), press filteration system (9), magnetic-particle synthesis system (12) and the magnetic-particle solution stocking system (6) that connect successively with little algae magnetic resolution collection device; Described magnetic-particle solution stocking system (6) is connected with mixing system (3);
Described frond gathering system (7) comprises the frond holding tank of at least two parallel connections; Mixing system (3), frond gathering system (7) and magnetic-particle solution stocking system (6) are equipped with whipping appts; Press filteration system (9) centre is provided with spray thrower (11); Magnetic-particle synthesis system (12) is provided with temperature regulating device;
Described magnetic resolution system comprises separator tank (19), rotary head drum (15), scraper plate (14), guide channel (13) and spray equipment group (20) for rotation magnetic separating device (4); The axis of described rotary head drum (15) is vertical with fluidic flow direction in the separator tank (19); Scraper plate (14) is tangent with the outer wall of rotary head drum (15); Guide channel (13) closely is connected with scraper plate (14), and becomes 5-30 ° of angle with horizontal plane; Spray equipment group (20) is parallel be located at scraper plate (14) directly over, it highly is lower than rotary head drum (15) upper edge;
Described rotary head drum (15) comprises fixedly inner core (16) and rotation urceolus (18); Fixedly inner core (16) lower surface is provided with bar permanent magnet (17), is arranged in parallel with rotary head drum (15) axis; The distance of rotation urceolus (18) lower edge to separator tank (19) bottom is 1-3cm;
Described bar permanent magnet (17) is oppositely arranged with scraper plate (14), and coverage is in 90 ° of scopes of axial plane below one side in the rotary head drum (15); Magneticstrength is 2000-5000Gs.
2. industrialization as claimed in claim 1 is characterized in that with little algae magnetic resolution collection device described mixing system (3) links to each other with reactor (1) by first feed pump (2-1); Described magnetic-particle solution stocking system (6) links to each other with mixing system (3) by second feed pump (2-2).
3. industrialization as claimed in claim 1 or 2 is characterized in that with little algae magnetic resolution collection device, and described magnetic resolution system links to each other with reactor (1) by the 3rd feed pump (2-3); Be provided with liquid meter (5) between magnetic resolution system and mixing system (3).
4. industrialization as claimed in claim 1 is characterized in that with little algae magnetic resolution collection device guide channel (13) becomes 15 ° of angles with horizontal plane.
5. industrialization as claimed in claim 1 is characterized in that with little algae magnetic resolution collection device the distance of rotation urceolus (18) lower edge to separator tank (19) bottom is 2cm.
6. industrialization as claimed in claim 1 is characterized in that with little algae magnetic resolution collection device described magneticstrength is 3500Gs.
As the described industrialization of one of claim 4-6 with little algae magnetic resolution collection device, it is characterized in that the fixedly inner core (16) of described rotary head drum (15) adopts technically pure iron or soft steel to make; Rotation urceolus (18) adopts non-magnetic stainless steel to make, and its sense of rotation is opposite with fluid flow direction.
As the described industrialization of one of claim 4-6 with little algae magnetic resolution collection device, it is characterized in that liquid levels is no more than axial plane height in the rotary head drum (15) in the described separator tank (19).
As the described industrialization of one of claim 1-2 with little algae magnetic resolution collection device, it is characterized in that described magnetic-particle is exposed nanometer Fe
3O
4Particle; The magnetic-particle synthesis system fills alkaline solution in (12).
10. industrialization as claimed in claim 9 is characterized in that with little algae magnetic resolution collection device described alkaline solution is NaOH solution or NH
3H
2O solution.
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| CN 201210013113 CN102586101B (en) | 2012-01-16 | 2012-01-16 | Industrial microalgae magnetic separation and collection device |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103194394A (en) * | 2013-04-10 | 2013-07-10 | 中国科学院过程工程研究所 | Method for separating microalgae by taking polyethyleneimine modified Fe3O4 nanoparticles as flocculating agent |
| CN103289895B (en) * | 2013-05-16 | 2014-12-24 | 中国科学院过程工程研究所 | Magnetic separating device and method for microalgae recovery |
| CN103333880A (en) * | 2013-06-19 | 2013-10-02 | 中国科学院过程工程研究所 | Method for harvesting microalgae with magnetic separator |
| TWI615467B (en) * | 2013-12-30 | 2018-02-21 | 國立交通大學 | Microalgae harvest module and method of harvesting microalgae |
| CN106362674B (en) * | 2016-09-11 | 2019-01-25 | 北京林业大学 | Synthesizing magnetic nano material in situ and the efficient method of recovery of oil algae in a kind of algae solution |
| CN110684655B (en) * | 2019-10-18 | 2023-03-17 | 江苏大学 | Microalgae separation gradient magnetic stabilization fluidized bed device and microalgae harvesting method thereof |
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| EP2336349A1 (en) * | 2008-09-26 | 2011-06-22 | Biótica, Bioquímica Analítica, S.L. | Rapid procedure for detection of microorganisms with magnetic particles |
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| US8206596B2 (en) * | 2008-02-22 | 2012-06-26 | Jiangsu Sinorgchem Technology Co., Ltd. | Magnetic separation apparatus and method for recovery of solid material from solid-liquid mixture |
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| EP2336349A1 (en) * | 2008-09-26 | 2011-06-22 | Biótica, Bioquímica Analítica, S.L. | Rapid procedure for detection of microorganisms with magnetic particles |
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