CN102580160A - Tissue engineering scaffold material of chemical bonding active material and preparation method thereof - Google Patents
Tissue engineering scaffold material of chemical bonding active material and preparation method thereof Download PDFInfo
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Abstract
The invention provides a tissue engineering scaffold material of a chemical bonding active material and a preparation method of the tissue engineering scaffold material. The tissue engineering scaffold material of the chemical bonding active material is prepared by a biodegradable macromolecular substance and an active material, wherein the macromolecular substance is counted by 100 parts by weight, and the active material is 0-15 part(s) by weight but is not zero; the 100 parts by weight of the macromolecular substance consists of 50-100 parts by weight of the synthesized macromolecular substance and 0-50 part(s) by weight of natural macromolecular substance; and a macromolecular nano-fiber film or a composite macromolecular nano-fiber film is formed by the macromolecular substance by means of electrostatic spinning, and the active material is evenly bonded on the surface of the fiber film. The tissue engineering scaffold material of the chemical bonding active material provided by the invention is good in biocompatibility, enough in mechanical strength, biodegradable, and suitable for stem cell proliferation and directed differentiation, and has the functions of cell migration adhesion promotion and stem cell capture to induce the regeneration of tissues such as bone, cartilage, nerve, skin, etc.
Description
Technical field
The present invention relates to biomaterial, particularly have biologic bracket material of catching stem cell and induced dry-cell propagation and directed differentiation function, tissue being had the regeneration induction effect and preparation method thereof and medical usage.
Background technology
In recent years, organize the notion of inductive biomaterial to form in the biomaterial scientific domain gradually.The key of tissue regeneration and reconstruction is the active timbering material of high biological response that the behavior of tissue Regeneration and Repair can be effectively induced in design.For tissue engineering bracket, because most of biologicallies all appear at surface or interface, thereby the surface nature of biomaterial and structure are very important to the biocompatibility of material.The surface property of embedded material has determined it to biomacromolecule in the body, absorption of proteins especially, and this can further determine in the body the various foreign body reactions to embedded material, thus the growth at interface between decision embedded material and the tissue.Between material and the cell the earliest reaction be by directly with the chemical composition of the material of body fluid and cells contacting, surface energy, roughness, and the topological structure on surface determine.In organizational project, cell behavior as adhere to, migration, differentiation, propagation and other all be closely related with the chemical physical property of material surface at the biological respinse at interface.Surface property such as hydrophilic, surface charge density, surface microscopic topographic, free energy and specific chemical group can influence the formation of synthetic and tissue topography of adhesion, cell enlargement, cell migration, differentiation, the extracellular matrix of cell.
In organizational project was used, electro spinning nano fiber support and extracellular matrix had higher similarity on morphosis, and its distinctive high-specific surface area and porosity help the adhesion and the propagation of cell.Electro spinning nano fiber be layer by layer random deposition on gathering-device, so its pore-size distribution broad (several microns to the hundreds of micron), but the size in most of holes is between 25～100 microns, this scope is suitable for the implantation and the growth of most cells.And the random orientation of fiber makes a little less than the interfibrous combination, can promote the fiber around its after in cell gets into the hole, and suitable adjustment aperture is with extending space, thereby improved the penetrating power of cell in three-dimensional rack.Consider the machinability of material, but electro spinning nanometer fiber membrane is main with the suitable synthetic high polymer of good spinnability bio-absorbable, mechanical property mainly, like polylactic acid (PLA), gather Acetic acid, hydroxy-, bimol. cyclic ester (PGA), poly (glycolide-lactide) (PLGA) and polycaprolactone (PCL) etc.Yet since the hydrophobicity on polyester material surface with lack the signal of cell recognition, thereby limited the application of said material in field of tissue engineering technology.Therefore, in order to regulate its cell/tissue function, introducing functional group on the polyester material surface through the method for compound or modification is the main method of its improvement in performance.
Studied the surface modification of PCL in concentrated NaOH solution like Schantz etc. and cultivated osteoblast with the surface property that changes polymer.Cell experiment is the result show, the PLGA that NaOH handles be untreated before compare, more help the adhesion and the propagation of VSMC.Lee etc. have reported the fixed biologically part (like biotin and polypeptide etc.) on the PGA surface, and fixed ligands can be through carboxylic acid group's reaction on surface after amino on the part and the basic hydrolysis of PGA stitching thread.And the method for hydrolysis or aminolysis can also be used for the surface modification of other polyester to regulate and control their application performance at biomedical sector.Yang etc. have reported that one is very simply improved the method for osteogen differentiation in PGA stitching thread surface regulation and control chemical property.This method comprises two steps, and the method through hydrolysis derives the carboxylic acid group on the surface, then through with the amino reaction, can be at surperficial covalent bond incoming fiber Fibronectin or RGD etc.The existence of polymer surfaces fiber adhesion albumen and rgd peptide can increase the propagation and the distribution of interstital stem cell, the differentiation that finally improves osteogen.Zhu etc. mentioned one simply and effectively fixedly gelatin or chitosan to the method on Biodegradable polyester surface.She reacts through the carboxyl on free amino group on the chitosan and the 4-azidobenzoic acid, and the 4-azidobenzoic acid is fixed on the chitosan.Utilize the heliosensitivity of 4-azidobenzoic acid, the employing irradiation under ultraviolet ray spreads upon the chitosan of PLA film surface, the azido group photodissociation, thus PLA and chitosan is covalently bound.Hydroxyl after the modification on the chitosan and the amino functional group that can introduce other again; Thereby can carry out further modification to PLA; As forming polyelectrolyte on the PLA surface after the further modification of heparin; Can reduce platelet in surface adhesion, and the heparin/chitosan/PLA surface adhesion force of cell after modification strengthens.Jee etc. have reported heparin have been keyed to the synthetic method on the PLA, to improve its blood compatibility and biocompatibility.The result shows, insert heparin a hydrophilic environment can be provided on the PLA surface, and heparin insert the PLA surface effectively CKIs matter show good anticoagulant property in its surperficial absorption and hematoblastic adhesion.
The present invention is from the microenvironment of stem cells hyperplasia and directed differentiation; Construction features and biotic environment requirement according to particular organization; With the biological degradable polyester kind macromolecular material is basic material; Pass through electrostatic spinning technique; Acquisition has excellent mechanical performances, controllable aperture, membrane support that porosity is adjustable; And the method through or surface modification natural polymer blended with other; Introduce functional groups on the timbering material surface; The functional groups of utilize keying in subsequently will move with stem cell, adhesion, natural bioactive material and active factors that proliferation and differentiation is relevant stably are anchored to the three-dimensional rack surface, regulating and control surface chemistry composition, topological structure and the microenvironment of this tissue engineering bracket, thereby construct have good biocompatibility, enough mechanical strength, biodegradable, be fit to stem cells hyperplasia and directed differentiation, have the adhesion of the cell migration of promotion and catch the tissue engineering bracket material that stem cell is induced the physically trapping active substance of function of tissue regeneration such as bone, cartilage, nerve and skin.
Summary of the invention
Thereby one of the object of the invention provides a kind of load has active substance, have good biocompatibility, enough mechanical strength, biodegradable, have and promote cell migration to adhere to and catch the tissue engineering bracket material of the chemical bonding active substance of functions such as stem cell induced tissue regeneration.
Two of the object of the invention provides the method for preparing of the tissue engineering bracket material of above-mentioned chemical bonding active substance.
For realizing above-mentioned purpose; The present invention adopts following technical scheme: a kind of tissue engineering bracket material of chemical bonding active substance; Being prepared from biodegradable polymer substance and active substance, is 100 weight portions in polymer substance, and active substance is a 0-15 weight portion but non-vanishing; Said 100 weight portion polymer substances are made up of the synthetic high polymer material of 50-100 weight portion and the natural high molecular substance of 0-50 weight portion;
Said biodegradable polymer substance forms high polymer nanometer fiber membrane or composite high-molecular nano fibrous membrane through electrostatic spinning, and said active substance evenly is bonded in the fibrous membrane surface.
The molecular weight of described synthetic high polymer material is 5～200,000, is selected from copolymer p LGA, polylactic acid, the polycaprolactone of lactic-co-glycolic acid or gathers one or more the mixture in the Acetic acid, hydroxy-, bimol. cyclic ester;
The molecular weight of described natural high molecular substance is 5～1,000,000, is selected from one or more the mixture in hyaluronic acid, fibroin, chondroitin sulfate, heparin, collagen protein, gelatin, chitosan, nucleic acid, fibronectin in serum or the polypeptide;
Described active substance is selected from epidermal growth factor EGF, fibroblast growth factor bFGF, endothelial cell growth factor VEGF, transforming growth factor TGF-β, insulin-like growth factor I GF, CD31 antibody, CD24 antibody, laminin, chemotactic factor SDF-1, nerve growth factor NGF, BMP BMP-2, osteogenic growth peptide OPG, platelet derived growth factor PDGF, contain one or more the mixture in the platelet rich plasma of multiple somatomedin.
A kind of method for preparing of the tissue engineering bracket material of above-mentioned chemical bonding active substance may further comprise the steps:
The preparation of a, macromolecule spinning liquid: synthetic high polymer material and natural high molecular substance mixed dissolution in solvent, are mixed with the macromolecule spinning liquid; Wherein the concentration of synthetic high polymer material is 0-100wt/v%, but non-vanishing, and the concentration of natural high molecular substance is 0-100wt/v%;
The preparation of b, timbering material: the electrostatic spinning apparatus of selecting many shower nozzles or single shower nozzle or nucleocapsid structure for use; Step a gained macromolecule spinning liquid packed into carry out electrostatic spinning in the device for storing liquid of electrospinning device; Obtain high polymer nanometer fiber membrane, fibre diameter is 10nm～1000nm;
C, the high polymer nanometer fiber membrane that step b is obtained are dipped in the cross-linking agent solution, natural high molecular substance component is wherein carried out crosslinked, in room temperature reaction after 6～12 hours, with a large amount of deionized water soaking flushing;
D, the high polymer nanometer fiber membrane after crosslinked is dipped in the aqueous solution that contains active substance; Utilize functional groups amino, hydroxyl or carboxyl in the natural high molecular substance strand; Through Electrostatic Absorption, hydrogen bond action or covalent bond effect active substance is bonded to the fibrous membrane surface, obtains can be used as the high polymer nanometer fiber membrane material of the tissue engineering bracket material of chemical bonding active substance.
A kind of method for preparing of the tissue engineering bracket material of above-mentioned chemical bonding active substance may further comprise the steps:
The preparation of a, macromolecule spinning liquid: the synthetic high polymer material is dissolved in first solvent, is mixed with first solution; Natural high molecular substance is dissolved in second solvent, is mixed with second solution;
The preparation of b, timbering material: select many shower nozzles device for spinning or nucleocapsid electric spinning equipment for use; The step solution that a wins and second solution is respectively charged in the different transfusion paths carries out electrostatic spinning; Obtain the composite high-molecular nano fibrous membrane; Wherein the diameter of synthetic high polymer fiber is 100-1000nm, and the diameter of natural polymer subbundle is 50-500nm, and the thickness of fibrous membrane is 0.1-0.5mm;
C, the composite high-molecular nano fibrous membrane that step b is obtained are dipped in the cross-linking agent solution, natural macromolecular material component is wherein carried out crosslinked, in room temperature reaction after 6～12 hours, with a large amount of deionized water soaking flushing;
D, the composite high-molecular nano fibrous membrane after crosslinked is dipped in the aqueous solution that contains active substance; Utilize functional groups amino, hydroxyl or carboxyl in the natural high molecular substance strand; Through Electrostatic Absorption, hydrogen bond action or covalent bond effect active substance is bonded to the fibrous membrane surface, obtains can be used as the composite high-molecular micro/nano fibrous membrane material of the tissue engineering bracket material of chemical bonding active substance.
A kind of method for preparing of the tissue engineering bracket material of above-mentioned chemical bonding active substance may further comprise the steps:
The preparation of a, synthetic high polymer nano fibrous membrane: the synthetic high polymer material is dissolved in the solvent, and being mixed with weight percent concentration is 6-15% macromolecule spinning liquid; Gained macromolecule spinning liquid packed into carry out electrostatic spinning in the device for storing liquid of electrospinning device, obtain the synthetic high polymer nano fibrous membrane, fibre diameter is 100nm～1000nm;
B, alkali treatment: configuration concentration is the sodium hydrate aqueous solution of 0.05-0.2M, and step a gained synthetic high polymer nano fibrous membrane is placed this solution, after 20～40 minutes, uses a large amount of deionized water rinsings in 0 ℃ of reaction;
C, contain the key entry of amino natural macromolecular: step b is put into the dehydrant carbodiimide that concentration is 50mM～200mM through the synthetic high polymer nano fibrous membrane of alkali treatment; In room temperature reaction after 8～16 hours; With a large amount of deionized water soaking flushing; Being immersed in concentration then is in the mixed solution that contains amino natural high molecular substance of 2%-5%, after room temperature reaction 6-12 hour, uses deionized water rinsing;
D, the synthetic high polymer nano fibrous membrane that key entry is contained behind the amino natural macromolecular are dipped in the aqueous solution that contains active substance; Utilize amino, the carboxyl of functional groups in the gelatin chains; Through Electrostatic Absorption, hydrogen bond action or covalent bond effect active substance is bonded to the fibrous membrane surface, obtains can be used as the high polymer nanometer fiber membrane material of the tissue engineering bracket material of chemical bonding active substance.
A kind of method for preparing of the tissue engineering bracket material of above-mentioned chemical bonding active substance may further comprise the steps:
The preparation of a, synthetic high polymer nano fibrous membrane: the synthetic high polymer material is dissolved in the solvent, and being mixed with weight percent concentration is 6-15% macromolecule spinning liquid; Gained macromolecule spinning liquid packed into carry out electrostatic spinning in the device for storing liquid of electrospinning device, obtain the synthetic high polymer nano fibrous membrane, fibre diameter is 100nm～1000nm;
B, alkali treatment: configuration concentration is the sodium hydrate aqueous solution of 0.05-0.2M, and step a gained synthetic high polymer nano fibrous membrane is placed this solution, after 20～40 minutes, uses a large amount of deionized water rinsings in 0 ℃ of reaction;
C, contain the key entry of amino natural macromolecular: step b is put into the dehydrant carbodiimide that concentration is 50mM～200mM through the synthetic high polymer nano fibrous membrane of alkali treatment; In room temperature reaction after 8～16 hours; With a large amount of deionized water soaking flushing; Being immersed in concentration then is in containing of 2%-5% of the amino natural polymer solution, after room temperature reaction 6-12 hour, uses deionized water rinsing;
Fixing of d, natural polymer spongy layer: configuration concentration is the natural polymer aqueous solution of 1%-6%, evenly is coated on the synthetic high polymer nano fibrous membrane of step c gained, and uses freeze dryer that solvent is extracted out;
E, natural polymer spongy layer crosslinked: the synthetic high polymer nano fibrous membrane that the steps d gained is coated with the natural polymer spongy layer is dipped in the cross-linking agent solution that concentration is 10mM～200mM; In room temperature reaction after 6～12 hours; With a large amount of deionized water soaking flushing; Place freezer dryer again, drain the moisture of absorption;
F, the synthetic high polymer nano fibrous membrane that will key in behind the natural polymer are dipped in the aqueous solution that contains active substance; Utilize amino, the carboxyl of functional groups in the gelatin chains; Through Electrostatic Absorption, hydrogen bond action active substance is bonded to the fibrous membrane surface, promptly obtains can be used as the high polymer nanometer fiber membrane material of the tissue engineering bracket material of chemical bonding active substance.
Described solvent is selected from one or more the mixture in fluoro reagent, chloroform, DMF, THF, ethanol, the methanol.
The process conditions of described electrostatic spinning are: the feeding rate of solution is 5～50ul/min, and the distance between the catcher of spinning head and ground connection is 8～20cm; Ambient temperature is 20～50 ℃; Electrostatic pressure is 10～30kV.
Described cross-linking agent solution is that temperature is 4 ℃ by the solution of the new preparation of mixed solvent of carbodiimide and acetone and water, and wherein the concentration of carbodiimide is 50mM～200mM, and in the mixed solvent of acetone and water, the weight ratio of acetone and water is 80: 20.
Described first solvent is selected from one or more the mixture among chloroform, DMF, the THF; Described second solvent is selected from water or water and alcoholic acid mixture or water and methanol mixture.
It is described that to contain amino natural polymer be gelatin, collagen, chitosan, hyaluronic acid etc.
The present invention has broken through organizational project research theory in the past; Break through the traditional view that the impossible induced tissue of abiotic material forms; Microenvironment from stem cells hyperplasia and directed differentiation; Construction features and biotic environment requirement according to particular organization; With the Biodegradable polymer material is basic material; Through technology such as nanometer electrospinning and material surface modifyings; Technology such as the design of the microcosmic microcellular structure of material and chemical modification are furtherd investigate, introduced natural activity material relevant and active factors etc., thereby design construction has gone out to have good biocompatibility, enough mechanical strength, biodegradable, suitable stem cells hyperplasia and directed differentiation, had the adhesion of promotion cell migration and catch the tissue engineering bracket material that stem cell is induced the chemical bonding active substance of function of tissue regeneration such as bone, cartilage, nerve and skin with stem cell migration, adhesion, proliferation and differentiation.Realize only can realizing the regeneration and the reconstruction of damaged tissues, for the development of regenerative medicine industry provides new approaches and new way through timbering material self.
Description of drawings
Fig. 1 is the cell density figure that in embodiment 1 gained tissue engineering bracket material, forms.
Fig. 2 is the figure of the SEM behind the PCL nano fibrous membrane grafted gelatin among the embodiment 3.
Fig. 3 is the section S EM figure of embodiment 4 gained PLGA/ gelatin-based porous support materials.
The specific embodiment
Below in conjunction with specific embodiment the present invention is elaborated.
(1) configuration of macromolecule spinning liquid: in TFE, being mixed with total concentration is the macromolecule spinning liquid of 12w/v% with PCL (molecular weight 100,000) and collagen protein (molecular weight 100,000) mixed dissolution, and wherein PCL and collagen mass ratio are 60: 40;
(2) preparation of micro/nano fibrous membrane material: step (1) gained macromolecule spinning liquid packed into carry out electrostatic spinning in the device for storing liquid of single nozzle electrospinning device, electric spinning process parameter is: electrostatic pressure is 20kV; Feeding rate is 50ul/min, and accepting distance is 15cm; Ambient temperature is 20 ℃, on catcher, collects the macromolecular fibre membrane material, and the average diameter of fiber is 550nm.
(3) the macromolecular fibre membrane material that step (2) is obtained is dipped in the mixed solution of 80/20 the second alcohol and water that contains the 100mM carbodiimide, the GE component is carried out crosslinked, in room temperature reaction after 12 hours, with a large amount of deionized water soaking flushing.
(4) the macromolecular fibre membrane material after crosslinked is dipped into 15h in the aqueous solution that contains 30% active substance (laminin, bFGF, EGF, CD24 antibody); Utilize in the collagen molecules chain remaining functional groups like amino, carboxyl etc.; Through hydrogen bond action bioactive molecule is bonded to the fibrous membrane surface, obtains the tissue engineering bracket material of a kind of chemical bonding active substance of the present invention.
(5) fibrous membrane that step (4) is prepared places and contains 1 * 10
3Carry out solid in the NSC of individual/ml (NSC) bioreactor and dynamically cultivate, different time takes out fibrous membrane and observes stem cell adhesion propagation situation.The result shows the prolongation along with incubation time; NSC on the fibrous membrane increases gradually; And the cell aixs cylinder that puts out the feelers is the neurocyte form and expresses neuron sign NSE and neurogliocyte sign GFAP; Show that constructed fibrous membrane can adhere to stem cell and promote its proliferation and differentiation, as shown in Figure 1.
Embodiment 2
(1) configuration of macromolecule spinning liquid: PLGA (molecular weight 100,000) is dissolved in the mixed solvent of 80/20 DMF and acetone, obtaining concentration is the PLGA solution of 12w/v%; Hyaluronic acid and gelatin are dissolved in the mixed solvent of 90/10 ethanol and water, prepare to such an extent that concentration is 5w/v%, hyaluronic acid and gelatin mass ratio are 30/70 mixed solution.
(2) preparation of micro/nano fibrous membrane material: select many shower nozzles device for spinning for use, PLGA solution is filled into respectively in the different transfusion paths with the mixed solution of hyaluronic acid and gelatin carries out electrostatic spinning, electric spinning process parameter is: electrostatic pressure is 25kV; Feeding rate is 20ul/min, and accepting distance is 8cm; Ambient temperature is 50 ℃, on catcher, obtains the macromolecular fibre membrane material, and the diameter of fiber is between 250～1000nm.
(3) fibrous membrane that step (2) is obtained is dipped in the mixed solution of 80/20 the second alcohol and water that contains the 200mM carbodiimide, hyaluronic acid and components of gelatin are carried out crosslinked, in room temperature reaction after 8 hours, with a large amount of deionized water soaking flushing.
(4) fibrous membrane after crosslinked is dipped into 15h in the aqueous solution of the 200mM dehydrant carbodiimide that contains 30% active substance (CD24 antibody, chemotactic factor SDF-1, epidermal growth factor EGF, fibroblast growth factor bFGF); Utilize in GE and the hyaluronan molecule chain remaining functional groups like amino, carboxyl etc.; Through the covalent bond effect active substance is bonded to the fibrous membrane surface, obtains the tissue engineering bracket material of a kind of chemical bonding active substance of the present invention.
(5) duplicating rabbit holostrome skin injury model (extracts bone marrow and extracts the cultivation mesenchymal stem cells MSCs before preparation skin injury model; And carry out GFP transgenic labelling); The fibrous membrane of step (4) preparation is covered in rabbit butt skin wound; 1,000,000 autologous bone marrow mesenchymal stem cells through the GFP labelling of intravenous injection simultaneously take out graft in different time in batches, and fluorescence microscope is observed stem cell migration adhesion situation down.The result finds that the green fluorescence cell is arranged on the fibrous membrane of transplanting, explains that constructed material can attract the stem cell migration to adhere to, and promptly has the effect of raising stem cell.
Embodiment 3
(1) preparation of synthetic high polymer nano fibrous membrane: PCL is dissolved in the mixed solvent of 80/20 DMF and methanol, the PCL solution that is mixed with concentration and is 12W/V% carries out electrostatic spinning, and electric spinning process parameter is: electrostatic pressure is 15kV; Feeding rate is 10ul/min, and accepting distance is 8cm; Ambient temperature is 20 ℃, on catcher, obtains the thick PCL micro/nano fibrous membrane material of 2-6mm.
(2) alkali treatment: the sodium hydrate aqueous solution of configuration 0.1M, the PCL micro/nano fibrous membrane material is placed this solution, after 20 minutes, use a large amount of deionized water rinsings in 0 ℃ of reaction.
(3) the macromolecular key entry of gelatin: it is 200mM dehydrant carbodiimide that the PCL micro/nano fibrous membrane material that step (2) is obtained is put into concentration; In room temperature reaction after 16 hours; With a large amount of deionized water soaking flushing; Be immersed in concentration then and be and carry out crosslinkedly in 5% the gelatin mixed solution, after 12 hours, use deionized water rinsing in room temperature reaction.Its SEM figure is as shown in Figure 2.
(4) the PCL micro/nano fibrous membrane material after crosslinked is dipped into 12h in the aqueous solution of the 50mM dehydrant carbodiimide that contains 30% active substance (endothelial cell growth factor VEGF, platelet rich plasma PRP); Utilize in the GE strand remaining functional groups like amino, carboxyl etc.; Through the covalent bond effect active substance is bonded to the fibrous membrane surface, obtains the tissue engineering bracket material of a kind of chemical bonding active substance of the present invention.
(5) duplicate new zealand white rabbit femur head necrosis animal model,, put to death animal in batches, observe the damaged reparation situation of bone in different time with the tissue engineering bracket material implantable bone defect of the chemical bonding active substance of step (4) preparation.The result finds that graft materials can significantly promote osteanagenesis and angiogenesis.
Embodiment 4
(1) preparation of synthetic high polymer nano fibrous membrane: PLGA is dissolved in the mixed solvent of 70/30 DMF and acetone, is mixed with concentration and is 15% PLGA solution and carry out electrostatic spinning, electric spinning process parameter is: electrostatic pressure is 20kV; Feeding rate is 20ul/min, and accepting distance is 8cm; Ambient temperature is 25 ℃, on catcher, obtains the PLGA fiber film material.
(2) configuration concentration is the sodium hydrate aqueous solution of 0.075M, and the PLGA fiber film material is placed this solution, after 30 minutes, uses a large amount of deionized water rinsings in 0 ℃ of reaction.
(3) the macromolecular key entry of gelatin: it is 50mM dehydrant carbodiimide that the PLGA fiber film material that step (2) is obtained is put into concentration; In room temperature reaction after 16 hours; With a large amount of deionized water soaking flushing; Be immersed in concentration then and be in 2% the aqueous gelatin solution, after 12 hours, use deionized water rinsing in room temperature reaction.
(4) gelatin-based biomacromolecule spongy layer is fixing: configuration concentration is 6% gelatin mixed solution, evenly is coated on step (3) the gained fibrous membrane, obtains the gelatin-compounded support of PLGA/, and uses freeze dryer that solvent is extracted out.
(5) the gelatin-based spongy layer is crosslinked: the gelatin-compounded support of PLGA/ that step (4) is obtained is dipped into and contains in the 200mM cross-linking agent solution (solvent: 80/20 ethanol and water); In room temperature reaction after 12 hours; With a large amount of deionized water soaking flushing; And fibrous framework placed freezer dryer once more, and drain the moisture of absorption, obtain PLGA/ gelatin-based porous support materials.Its SEM figure is as shown in Figure 3.
(6) the PLGA/ gelatin-based porous support materials after crosslinked is dipped into 12h in the aqueous solution that contains 30% bioactive molecule (endothelial cell growth factor VEGF, CD31 antibody); Utilize functional groups in the GE strand like amino, carboxyl etc.; Through hydrogen bond action active substance is bonded to PLGA/ gelatin-based porous support surface, obtains the tissue engineering bracket material of a kind of chemical bonding active substance of the present invention.
(5) tissue engineering bracket material of the chemical bonding active substance of step (6) preparation is placed contain 1 * 10
3Carry out solid in the mesenchymal stem cells MSCs of individual/ml (MSC) bioreactor and dynamically cultivate, different time takes out fibrous membrane and observes stem cell adhesion proliferation and differentiation situation.The result shows the prolongation along with incubation time, and the cell on the fibrous membrane increases gradually, and expresses endotheliocyte sign F8 and CD31, shows that this support can promote stem cell to adhere to propagation and breaks up to the endotheliocyte direction.
Embodiment 5
(1) preparation of synthetic high polymer nano fibrous membrane: PLA is dissolved in the mixed solvent of 80/20 DMF and chloroform, the PLA solution that is mixed with concentration and is 10W/V% carries out electrostatic spinning, and electric spinning process parameter is: electrostatic pressure is 15kV; Feeding rate is 10ul/min, and accepting distance is 8cm; Ambient temperature is 20 ℃, on catcher, obtains the thick PCL micro/nano fibrous membrane material of 0.15mm.
(2) alkali treatment: the sodium hydrate aqueous solution of configuration 0.1M, the PLA micro/nano fibrous membrane material is placed this solution, after 20 minutes, use a large amount of deionized water rinsings in 0 ℃ of reaction.
(3) the macromolecular key entry of chitosan: it is 200mM dehydrant carbodiimide that the PLA micro/nano fibrous membrane material that step (2) is obtained is put into concentration; In room temperature reaction after 16 hours; With a large amount of deionized water soaking flushing; Be immersed in concentration then and be and carry out crosslinkedly in 5% the chitosan mixed solution, after 12 hours, use deionized water rinsing in room temperature reaction.
(4) the PLA micro/nano fibrous membrane material after crosslinked is dipped into 12h in the aqueous solution of the 50mM dehydrant carbodiimide that contains 30% active substance (fibroblast growth factor bFGF, endothelial cell growth factor VEGF or a certain proportion of platelet rich plasma PRP); Utilize in the GE strand remaining functional groups like amino, carboxyl etc.; Through the covalent bond effect active substance is bonded to the fibrous membrane surface, obtains the tissue engineering bracket material of a kind of chemical bonding active substance of the present invention.
(5) duplicate new zealand white rabbit holostrome skin injury animal model, transplant in the skin injury place after the high polymer nanometer fiber membrane materials disinfection sterilization with preparation, observe the reparation situation of damaged skin respectively at different time.The result is along with the time increases, and the white fiber graft is degraded gradually, and wound surface and graft are fresh moistening, and 4 all left and right sides grafts are completed into rebirth skin; Frozen section HE coloration result shows that the rebirth skin structure and the normal skin that form are basic identical, has formed normal epidermal area, hypodermis layer and skin corium, and it is thus clear that skin appendages---formation such as hair, sebaceous gland, sweat gland is arranged.
Embodiment 6
(1) preparation of synthetic high polymer nano fibrous membrane: PLGA is dissolved in the mixed solvent of 70/30 DMF and acetone, is mixed with concentration and is 8% PLGA solution and carry out electrostatic spinning, electric spinning process parameter is: electrostatic pressure is 20kV; Feeding rate is 20ul/min, and accepting distance is 8cm; Ambient temperature is 25 ℃, on catcher, obtains the PLGA fiber film material.
(2) configuration concentration is the sodium hydrate aqueous solution of 0.075M, and the PLGA fiber film material is placed this solution, after 30 minutes, uses a large amount of deionized water rinsings in 0 ℃ of reaction.
(3) the macromolecular key entry of gelatin: it is 50mM dehydrant carbodiimide that the PLGA fiber film material that step (2) is obtained is put into concentration; In room temperature reaction after 16 hours; With a large amount of deionized water soaking flushing; Be immersed in concentration then and be in 2% the aqueous gelatin solution, after 12 hours, use deionized water rinsing in room temperature reaction.
(4) alginic acid sodio biomacromolecule spongy layer is fixing: configuration concentration is 4% sodium alginate soln, evenly is coated on step (3) the gained fibrous membrane, obtains PLGA/ sodium alginate compound rest, and uses freeze dryer that solvent is extracted out.
(5) alginic acid sodio spongy layer is crosslinked: the gelatin-compounded support of PLGA/ that step (4) is obtained is dipped into and contains 200mM CaCl
2In the solution (solvent: 80/20 ethanol and water), in room temperature reaction after 12 hours,, and fibrous framework placed freezer dryer once more, drain the moisture of absorption, obtain hole, PLGA/ sodium alginate Quito timbering material with a large amount of deionized water soaking flushing.
(6) hole, the PLGA/ sodium alginate Quito timbering material after crosslinked is dipped into 12h in the aqueous solution that contains 30% bioactive molecule (BMP BMP-2, transforming growth factor TGF-β or a certain proportion of platelet rich plasma PRP); Utilize functional groups such as hydroxyl, carboxyl etc. in the sodium alginate strand; Through hydrogen bond action active substance is bonded to hole, PLGA/ sodium alginate Quito rack surface, obtains the tissue engineering bracket material of a kind of chemical bonding active substance of the present invention.
(5) duplicate the damaged animal model of new zealand white rabbit knee cartilage, the composite high-molecular micro/nano fibrous membrane material of preparation is transplanted in cartilage defect place, put to death animal in batches, observe the repairing effect of graft damaged cartilage in different time.The result shows that the timbering material that contains bioactive molecule can significantly promote the propagation of injury region chondrocyte and the reparation of cartilaginous tissue.
Claims (10)
1. the tissue engineering bracket material of a chemical bonding active substance, it is characterized in that: being prepared from biodegradable polymer substance and active substance, is 100 weight portions in polymer substance, active substance is a 0-15 weight portion but non-vanishing; Said 100 weight portion polymer substances are made up of the synthetic high polymer material of 50-100 weight portion and the natural high molecular substance of 0-50 weight portion;
Said biodegradable polymer substance forms high polymer nanometer fiber membrane or composite high-molecular nano fibrous membrane through electrostatic spinning, and said active substance evenly is bonded in the fibrous membrane surface.
2. the tissue engineering bracket material of chemical bonding active substance as claimed in claim 1; It is characterized in that: the molecular weight of described synthetic high polymer material is 5～200,000, is selected from copolymer p LGA, polylactic acid, the polycaprolactone of lactic-co-glycolic acid or gathers one or more the mixture in the Acetic acid, hydroxy-, bimol. cyclic ester;
The molecular weight of described natural high molecular substance is 5～1,000,000, is selected from one or more the mixture in hyaluronic acid, fibroin, chondroitin sulfate, heparin, collagen protein, gelatin, chitosan, nucleic acid, fibronectin in serum or the polypeptide;
Described active substance is selected from epidermal growth factor EGF; Fibroblast growth factor bFGF; Endothelial cell growth factor VEGF; Transforming growth factor TGF-β; Insulin-like growth factor I GF; CD31 antibody; CD24 antibody; Laminin; Chemotactic factor S D F-1; Nerve growth factor NGF; BMP BMP-2; Osteogenic growth peptide OPG; Platelet derived growth factor PDGF; Contain one or more the mixture in the platelet rich plasma of multiple somatomedin.
3. the method for preparing of the tissue engineering bracket material of chemical bonding active substance as claimed in claim 1 is characterized in that, may further comprise the steps:
The preparation of a, macromolecule spinning liquid: synthetic high polymer material and natural high molecular substance mixed dissolution in solvent, are mixed with the macromolecule spinning liquid; Wherein the concentration of synthetic high polymer material is 0-100wt/v%, but non-vanishing, and the concentration of natural high molecular substance is 0-100wt/v%;
The preparation of b, timbering material: the electrostatic spinning apparatus of selecting many shower nozzles or single shower nozzle or nucleocapsid structure for use; Step a gained macromolecule spinning liquid packed into carry out electrostatic spinning in the device for storing liquid of electrospinning device; Obtain high polymer nanometer fiber membrane, fibre diameter is 50nm～1000nm;
C, the high polymer nanometer fiber membrane that step b is obtained are dipped in the cross-linking agent solution, natural high molecular substance component is wherein carried out crosslinked, in room temperature reaction after 6～12 hours, with a large amount of deionized water soaking flushing;
D, the high polymer nanometer fiber membrane after crosslinked is dipped in the aqueous solution that contains active substance; Utilize functional groups amino, hydroxyl or carboxyl in the natural high molecular substance strand; Through Electrostatic Absorption, hydrogen bond action or covalent bond effect active substance is bonded to the fibrous membrane surface, obtains can be used as the high polymer nanometer fiber membrane material of the tissue engineering bracket material of chemical bonding active substance.
4. the method for preparing of the tissue engineering bracket material of chemical bonding active substance as claimed in claim 1 is characterized in that, may further comprise the steps:
The preparation of a, macromolecule spinning liquid: the synthetic high polymer material is dissolved in first solvent, is mixed with first solution; Natural high molecular substance is dissolved in second solvent, is mixed with second solution;
The preparation of b, timbering material: select many shower nozzles device for spinning or nucleocapsid electric spinning equipment for use; The step solution that a wins and second solution is respectively charged in the different transfusion paths carries out electrostatic spinning; Obtain the composite high-molecular nano fibrous membrane; Wherein the diameter of synthetic high polymer fiber is 100-1000nm, and the diameter of natural polymer subbundle is 50-500nm, and the thickness of fibrous membrane is 0.1-0.5mm;
C, the composite high-molecular nano fibrous membrane that step b is obtained are dipped in the cross-linking agent solution, natural macromolecular material component is wherein carried out crosslinked, in room temperature reaction after 6～12 hours, with a large amount of deionized water soaking flushing;
D, the composite high-molecular nano fibrous membrane after crosslinked is dipped in the aqueous solution that contains active substance; Utilize functional groups amino, hydroxyl or carboxyl in the natural high molecular substance strand; Through Electrostatic Absorption, hydrogen bond action or covalent bond effect active substance is bonded to the fibrous membrane surface, obtains can be used as the composite high-molecular micro/nano fibrous membrane material of the tissue engineering bracket material of chemical bonding active substance.
5. the method for preparing of the tissue engineering bracket material of chemical bonding active substance as claimed in claim 1 is characterized in that, may further comprise the steps:
The preparation of a, synthetic high polymer nano fibrous membrane: the synthetic high polymer material is dissolved in the solvent, and being mixed with weight percent concentration is 6-15% macromolecule spinning liquid; Gained macromolecule spinning liquid packed into carry out electrostatic spinning in the device for storing liquid of electrospinning device, obtain the synthetic high polymer nano fibrous membrane, fibre diameter is 100nm～1000nm;
B, alkali treatment: configuration concentration is the sodium hydrate aqueous solution of 0.05-0.2M, and step a gained synthetic high polymer nano fibrous membrane is placed this solution, after 20～40 minutes, uses a large amount of deionized water rinsings in 0 ℃ of reaction;
C, contain the key entry of amino natural macromolecular: step b is put into the dehydrant carbodiimide that concentration is 50mM～200mM through the synthetic high polymer nano fibrous membrane of alkali treatment; In room temperature reaction after 8～16 hours; With a large amount of deionized water soaking flushing; Being immersed in concentration then is in the mixed solution that contains amino natural high molecular substance of 2%-5%, after room temperature reaction 6-12 hour, uses deionized water rinsing;
D, the synthetic high polymer nano fibrous membrane that key entry is contained behind the amino macromole are dipped in the aqueous solution that contains active substance; Utilize amino, the carboxyl of functional groups in the gelatin chains; Through Electrostatic Absorption, hydrogen bond action or covalent bond effect active substance is bonded to the fibrous membrane surface, obtains can be used as the high polymer nanometer fiber membrane material of the tissue engineering bracket material of chemical bonding active substance.
6. the method for preparing of the tissue engineering bracket material of chemical bonding active substance as claimed in claim 1 is characterized in that, may further comprise the steps:
The preparation of a, synthetic high polymer nano fibrous membrane: the synthetic high polymer material is dissolved in the solvent, and being mixed with weight percent concentration is 6-15% macromolecule spinning liquid; Gained macromolecule spinning liquid packed into carry out electrostatic spinning in the device for storing liquid of electrospinning device, obtain the synthetic high polymer nano fibrous membrane, fibre diameter is 100nm～1000nm;
B, alkali treatment: configuration concentration is the sodium hydrate aqueous solution of 0.05-0.2M, and step a gained synthetic high polymer nano fibrous membrane is placed this solution, after 20～40 minutes, uses a large amount of deionized water rinsings in 0 ℃ of reaction;
C, contain the key entry of amino natural macromolecular: step b is put into the dehydrant carbodiimide that concentration is 50mM～200mM through the synthetic high polymer nano fibrous membrane of alkali treatment; In room temperature reaction after 8～16 hours; With a large amount of deionized water soaking flushing; Being immersed in concentration then is in containing of 2%-5% of the amino natural polymer solution, after room temperature reaction 6-12 hour, uses deionized water rinsing;
Fixing of d, natural polymer spongy layer: configuration concentration is the natural polymer aqueous solution of 1%-6%, evenly is coated on the synthetic high polymer nano fibrous membrane of step c gained, and uses freeze dryer that solvent is extracted out;
E, natural polymer spongy layer crosslinked: the synthetic high polymer nano fibrous membrane that the steps d gained is coated with the natural polymer spongy layer is dipped in the cross-linking agent solution that concentration is 10mM～200mM; In room temperature reaction after 6～12 hours; With a large amount of deionized water soaking flushing; Place freezer dryer again, drain the moisture of absorption;
F, the synthetic high polymer nano fibrous membrane that will key in behind the natural polymer are dipped in the aqueous solution that contains active substance; Utilize amino, the carboxyl of functional groups in the natural molecule chain; Through Electrostatic Absorption, hydrogen bond action active substance is bonded to the fibrous membrane surface, promptly obtains can be used as the high polymer nanometer fiber membrane material of the tissue engineering bracket material of chemical bonding active substance.
7. like the tissue engineering bracket material of claim 3 or 5 or 6 described chemical bonding active substances, it is characterized in that: described solvent is selected from one or more the mixture in fluoro reagent, chloroform, DMF, THF, ethanol, the methanol.
8. like claim 3 or 4 or the tissue engineering bracket material of 5 or 6 described chemical bonding active substances; It is characterized in that: the process conditions of described electrostatic spinning are: the feeding rate of solution is 5～50ul/min, and the distance between the catcher of spinning head and ground connection is 8～20cm; Ambient temperature is 20～50 ℃; Electrostatic pressure is 10～30kV.
9. like the tissue engineering bracket material of claim 3 or 4 or 6 described chemical bonding active substances; It is characterized in that: described cross-linking agent solution is the solution by the new preparation of mixed solvent of carbodiimide and acetone and water; Temperature is 4 ℃; Wherein the concentration of carbodiimide is 50mM～200mM, and in the mixed solvent of acetone and water, the weight ratio of acetone and water is 80: 20.
10. the tissue engineering bracket material of chemical bonding active substance as claimed in claim 4 is characterized in that: described first solvent is selected from one or more the mixture among chloroform, DMF, the THF; Described second solvent is selected from water or water and alcoholic acid mixture or water and methanol mixture.
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