CN102565272A - Quality standard of standardized bupleurum extract - Google Patents
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Abstract
The invention relates to a method for controlling the quality of a standardized extract of a Chinese herbal medicine, i.e., the quality standard of standardized bupleurum extract. The method comprises the following steps: the bupleurum chinense is extracted in the semi-bionic environment, the total content of the main effective components (saikosaponin a, saikosaponin b, saikosaponin c and saikosaponin d) of the extract is equal to or greater than 1.874 percent, and the total content is equal to or greater than 1.3 percent according to the standard content index. The quality standard of the standardized bupleurum extract includes identification of bupleurum extract, content determination of saikosaponins, specific chromatogram determination and other detection items. The main components of the bupleurum extract can be determined qualitatively and quantitatively, the unified and controllable quality and the standardize production of the bupleurum extract can be ensured, the pharmaceutical quality and the therapeutic effect of the bupleurum extract are guaranteed, and a new quality standard is formulated for completely and accurately evaluating the quality of the standardized bupleurum extract on the basis of experimental research. The method has the characteristics of simplicity, convenience, stability and good reproducibility, and is favorable for improving the inspection efficiency.
Description
Technical field
The present invention relates to a kind of standard and preparation method of Chinese crude drug extract, i.e. radix bupleuri standardized extract quality standard.
Background technology
In the prior art, the dry root of umbelliferae bupleurum Bupleurum chinense DC. through being processed into extract, is the yellowish-brown powder, the little perfume (or spice) of gas, mildly bitter flavor.Radix bupleuri or title Bupleurum Chinese, RADIX BUPLEURI SCORZONERAEFOLII.The function of bupleurum extract with cure mainly for: evacuate and to bring down a fever soothing liver-qi stagnation, elevate a turnable ladder yang-energy.Be used for cold, fever, fevers and chills alternate, sternal rib pain, irregular menstruation, the irregular menstruation prolapse of uterus, prolapse of the anus.Bupleurum extract market supply mostly is a water extract, saikoside on HPLC, detect less than, its effective constituent does not have the qualitative, quantitative standard; Only provide specification: 5: 1,10: 1,20: 1 (getting the powder rate); Can not guarantee every batch of product quality,, influence the medicine product quality as bulk drug.
The composition of radix bupleuri mainly contains saikoside saponin(es such as (a, b, c, d), sterol, volatile oil (bupleurumol, eugenol etc.), fatty acid (oleic acid, linolenic acid, palmitic acid, stearic acid etc.) and polysaccharide etc.; That wherein mainly play therapeutic action is saikoside (a, b, c, d), has to evacuate to bring down a fever soothing liver-qi stagnation, the effect of elevate a turnable ladder yang-energy; Can treat cold, fever, fevers and chills alternate, sternal rib pain, irregular menstruation; The irregular menstruation prolapse of uterus, illnesss such as prolapse of the anus, medical value is very high.Particularly water extracts when routine is extracted; Under this environmental baseline, extract, can make the fracture of primary glycosides epoxy ehter bond, the extraction ratio of main pharmacodynamics composition saikoside (a, b, c, d) is low extremely low; Thin layer is inspected and HPLC detects; Almost do not have display dot and the characteristic absorption peak of saikoside (a, b, c, d), the main pharmacodynamics composition is lost, the weak effect of curing the disease.
Summary of the invention
The objective of the invention is to above-mentioned deficiency and provide a kind of in the radix bupleuri standardized extract, saikoside (a, b, c, d) differentiates and assay technical indicator control method, the quality of assurance radix bupleuri standardized extract and the quality standard formulated.
Technical solution of the present invention is: the control method of bupleurum extract discriminating and assay index is characterized in that step is following:
(1) bupleurum extract preparation: get Bupleurum Chinese and be ground into powdery (meal), it is an amount of to add 60% ethanol, transfers PH=8 with stabilizing agent (1.5%KOH), soaks into 8h; Under 40 ℃ of conditions of temperature, ultrasonic (power 250W, 50kHz) extracts 3 times, each 40 minutes, adds 8 times of amount ethanol at every turn; Transfer PH=8 with stabilizing agent (1.5%KOH), filter merging filtrate; Reclaim ethanol to there not being the alcohol flavor, be concentrated into relative density 1.10 (60 ℃) spray drying or micro-wave vacuum, promptly get.Batch process selects for use FGC-TQ/4/1.6/3.6 still pot type Extraction by Ultrasound, vacuum to be condensed into complete equipment.
Half biomimetic method prepares radix bupleuri standardized extract principle, mainly is under the condition of constant body temperature of bionical object and biosome large intestine ecologic environment pH value, in conjunction with other process conditions, makes the orthogonal experiment environment, is set under the half bionical environment and extracts screening.Through detecting; Bupleurum extract contains four saikoside monomer saikoside a, saikoside b, saikoside c, saikoside d sum >=1.874%; Demonstrate fully the strict quality standard requirement of Chinese medicine standardized extract; Except that having former plant same function function, also can be used for doing the bulk drug of preparation.
(2) differentiate:
The preparation of need testing solution: get bupleurum extract 0.6g, add methyl alcohol 10ml, sonicated 2min filters, as need testing solution;
The preparation of saikoside reference substance solution: get saikoside a reference substance, saikoside d reference substance, saikoside b reference substance, saikoside c reference substance, add methyl alcohol and process the mixed solution that every 1ml contains 0.4mg, as reference substance solution;
" each 10 μ l of above-mentioned solution are drawn in an appendix VI of Chinese pharmacopoeia version in 2010 B test, put respectively on same silica gel g thin-layer plate according to thin-layered chromatography; With ethyl acetate, ethanol, water=8: 2: 1 was developping agent, launched, and took out; Dry; It is clear that spray is heated to the spot colour developing with 40% sulfuric acid solution of 2% pair of dimethylbenzaldehyde at 60 ℃, under daylight and ultraviolet lamp 365n m, inspects respectively, in the test sample chromatogram; Be on the reference substance chromatogram relevant position, show the spot or the fluorescence spot of same color;
(3) saikoside assay: " appendix VID of Chinese pharmacopoeia version in 2010 measures according to high performance liquid chromatography; Chromatographic condition and system suitability test are carried out according to assay under the radix bupleuri item;
The preparation of reference substance solution: get saikoside a reference substance, saikoside d reference substance, saikoside c reference substance, saikoside b reference substance are an amount of, accurately claim surely, add methyl alcohol and process the solution that every 1ml contains each 0.4mg of saikoside, shake up, and promptly get;
The preparation of need testing solution: get the about 0.6g of these article powder, the accurate title, decide, and puts in the 20ml measuring bottle, adds methyl alcohol to scale, and ultrasonic 10 minutes, filter, get subsequent filtrate, promptly get;
Determination method: accurate respectively reference substance solution 10ul and the need testing solution 10ul of drawing, inject liquid chromatograph, measure, promptly get;
These article are pressed dry product and are calculated, and contain saikoside a (C
42H
68O
13), saikoside d (C
42H
68O
13) total amount of saikoside c, saikoside b must not be less than 1.30%.
(4) " contrast characteristic peak collection of illustrative plates ", " characteristic peak coincide collection of illustrative plates " mensuration
" appendix VID of Chinese pharmacopoeia version in 2010 measures according to high performance liquid chromatography;
Chromatographic condition and system suitability test: with the octadecylsilane chemically bonded silica is filling agent; With the acetonitrile is mobile phase A, is Mobile phase B with water, and the regulation in the according to the form below is carried out gradient elution; The detection wavelength is 210nm, and number of theoretical plate calculates by saikoside a peak should be not less than 10000;
The preparation of object of reference solution: it is an amount of to get saikoside a reference substance, saikoside b reference substance, saikoside c reference substance, saikoside d reference substance; The accurate title, decide; Put in the 10ml measuring bottle; Add dissolve with methanol and be diluted to and contain the mixed solution of getting saikoside a 0.4mg, saikoside b 0.5mg, saikoside c 3mg, saikoside d 0.5mg among every 1ml, shake up, promptly get;
The preparation of need testing solution: get the about 0.6g of these article, the accurate title, decide, and puts in the 20ml measuring bottle, adds methyl alcohol to scale, and ultrasonic 10 minutes, filter, get subsequent filtrate, promptly get;
Accurate respectively object of reference solution and each 20ul of need testing solution of drawing of characteristic spectrum determination method injects liquid chromatograph, measures, and writes down 55 minutes chromatogram, promptly gets characteristic spectrum;
The accurate respectively absorption object of reference solution of the identical collection of illustrative plates determination method of characteristic peak and each 10ul of need testing solution inject liquid chromatograph after mixing, and measure, and write down 55 minutes chromatogram, promptly get the identical collection of illustrative plates of characteristic peak.
Should present 4 characteristic peaks in the test sample characteristic spectrum; With the corresponding peak of object of reference be the S peak; Calculate each characteristic peak and the corresponding peak-to-peak relative retention time of contrast characteristic spectrum; 3 peaks should be identical with corresponding object of reference peak retention time respectively therein, its relative retention time should setting ± 5% within; Setting is: 17.133 (peaks 1), 20.950 (peaks 2), 21.167 (peaks 3), 26.467 (peaks 4).
Advantage of the present invention is: the principal ingredient of definite bupleurum extract that 1, can qualitative, quantitative; Guaranteed that the bupleurum extract quality is unified, controlled; Standard is produced, and guarantees the drug quality and the curative effect of bupleurum extract, can effectively, reliably guarantee the product quality of bupleurum extract; For GMP production standard bupleurum extract, new quality control standard is provided.2, this method has easy, stable, favorable reproducibility, characteristics that specificity is strong, helps improving checkability.
To combine embodiment that embodiment of the present invention is described in further detail below.
Description of drawings
Fig. 1 is that test sample is measured collection of illustrative plates in the assay,
Fig. 2 is " a contrast characteristic spectrum ",
Fig. 3 is " characteristic peak coincide collection of illustrative plates ",
Fig. 4 is a TLC thin layer chromatic graph.
Embodiment
1: three batch of stable scale-up of embodiment
Radix bupleuri standardized extract half bionical combined ultrasonic preparation method: get Bupleurum Chinese 250g and be ground into meal, it is an amount of to add 60% ethanol, transfers PH=8 with stabilizing agent (1.5%KOH); Soak into 8h, under 40 ℃ of conditions of temperature, ultrasonic (power 250W, 50kHz) extracts 3 times; Each 40 minutes, add 8 times of amount ethanol at every turn, transfer PH=8 with stabilizing agent (1.5%KOH); Filter, merging filtrate reclaims ethanol to there not being the alcohol flavor; Be concentrated into relative density 1.10 (60 ℃) spray drying or micro-wave vacuum, promptly get (seeing table 2).
The average flour extraction of the three batches of amplification tests 15.35%, with saikoside abcd total content calculate that its extract average content is 1.874%, average yield is 0.288%, extraction ratio is 84.706%.See table 2.
Three batches of amplification test main technologic parameters of table 2
Table 1 orthogonal test main technologic parameters:
2, method design concept and target,
According to orthogonal test (seeing table 1) and three batches of preferred process conditions of stable amplification test, formulate radix bupleuri standardized extract preparation method.
Half biomimetic method prepares radix bupleuri standardized extract principle, mainly is under the condition of constant body temperature of bionical object and biosome large intestine ecologic environment pH value, in conjunction with other process conditions, makes the orthogonal experiment environment, is set under the half bionical environment and extracts screening.
The leaching process of purpose of design under this environmental baseline can be avoided the fracture of primary glycosides epoxy ehter bond; Primary glycosides epoxy ehter bond is constantly split and a large amount of acetyl group saikoside (2-0-acetyl group saikoside a, 2-0-acetyl group saikoside b2,3-0-acetyl group saikoside b2 etc.) deacetylate can be made; Make it become corresponding saikoside; Can increase substantially the yield of principal ingredient like this; And routine is extracted particularly water extraction, inspects and the HPLC detection at thin layer, does not almost have display dot and the absorption peak of saikoside abcd.The main pharmacodynamics composition is lost.Under half bionical environment, extract; Can make the extraction rate reached to 84.706% of main pharmacodynamics composition saikoside abcd; And acid impurities in the viscous polysaccharide and alkali generate deposition quickening filtyration velocity; Reduce the water-solubility impurity in the filtrating, thereby reach the purpose of improving the quality of products with the yield of product.
The content's index control of radix bupleuri standardized extract: with saikoside abcd sum >=1.3% is the content controlling index.
The radix bupleuri standardized extract differentiates, adopts " characteristic peak coincide collection of illustrative plates " " characteristic peak contrast collection of illustrative plates " " TLC chromatogram. " to carry out quality control.All accomplish good reproducibility, specificity is strong, has accomplished 4 peaks of saikoside, fits like a glove, and retention time is identical.
Detection method is stable, accurately, can repeat; Test detects the method adopted and is " official method that Chinese pharmacopoeia version in 2010 is recorded, " feature comparison collection of illustrative plates " " thin layer collection of illustrative plates " of test findings RSD1.212% test findings and " characteristic peak coincide collection of illustrative plates "
Embodiment 2
The bupleurum extract quality standard comprises the projects such as the identical collection of illustrative plates mensuration of discriminating, saikoside assay, characteristic spectrum and characteristic peak of bupleurum extract.
Bupleurum extract differentiates and content assaying method that its step is following:
(1) bupleurum extract preparation: get Bupleurum Chinese 250g and be ground into meal, it is an amount of to add 60% ethanol, transfers pH=8 with stabilizing agent (1.5%KOH), soaks into 8h; Under 40 ℃ of conditions of temperature, ultrasonic (power 250W, 50kHz) extracts 3 times, each 40 minutes, adds 8 times of amount ethanol at every turn; Transfer pH=8 with stabilizing agent (1.5%KOH), filter merging filtrate; Reclaim ethanol to there not being the alcohol flavor, be concentrated into relative density 1.10 (60 ℃) spray drying or micro-wave vacuum, promptly get (seeing table 2).
(2) differentiate:
The preparation of need testing solution: get bupleurum extract 0.6g, add methyl alcohol 10ml, sonicated 2min filters, as need testing solution;
The preparation of saikoside reference substance solution: get saikoside a reference substance, saikoside d reference substance, saikoside b reference substance, saikoside c reference substance, add methyl alcohol and process the mixed solution that every 1ml contains 0.4mg, as reference substance solution;
" each 10 μ l of above-mentioned solution are drawn in an appendix VI of Chinese pharmacopoeia version in 2010 B test, put respectively on same silica gel g thin-layer plate according to thin-layered chromatography; With ethyl acetate, ethanol, water=8: 2: 1 was developping agent, launched, and took out; Dry; It is clear that spray is heated to the spot colour developing with 40% sulfuric acid solution of 2% pair of dimethylbenzaldehyde at 60 ℃, under daylight and ultraviolet lamp 365n m, inspects respectively, in the test sample chromatogram; Be on the reference substance chromatogram relevant position, show the spot or the fluorescence spot of same color;
(3) saikoside assay: " appendix VID of Chinese pharmacopoeia version in 2010 measures according to high performance liquid chromatography; Chromatographic condition and system suitability test are carried out according to assay under the radix bupleuri item;
The preparation of reference substance solution: get saikoside a reference substance, saikoside d reference substance, saikoside c reference substance, saikoside b reference substance are an amount of, accurately claim surely, add methyl alcohol and process the solution that every 1ml contains each 0.4mg of saikoside, shake up, and promptly get;
The preparation of need testing solution: get the about 0.6g of these article powder, the accurate title, decide, and puts in the 20ml measuring bottle, adds methyl alcohol to scale, and ultrasonic 10 minutes, filter, get subsequent filtrate, promptly get;
Determination method: accurate respectively reference substance solution 10ul and the need testing solution 10ul of drawing, inject liquid chromatograph, measure, promptly get, see Fig. 1.
These article are pressed dry product and are calculated, and contain saikoside a (C
42H
68O
13), saikoside d (C
42H
68O
13) total amount of saikoside c, saikoside b must not be less than 1.3%.
(4) " characteristic spectrum " " characteristic peak coincide collection of illustrative plates " measured: " appendix VID of Chinese pharmacopoeia version in 2010 measures according to high performance liquid chromatography;
Chromatographic condition and system suitability test: with the octadecylsilane chemically bonded silica is filling agent; With the acetonitrile is mobile phase A, is Mobile phase B with water, and the regulation in the according to the form below is carried out gradient elution; The detection wavelength is 210nm, and number of theoretical plate calculates by saikoside a peak should be not less than 10000;
The preparation of object of reference solution: it is an amount of to get saikoside a reference substance, saikoside b reference substance, saikoside c reference substance, saikoside d reference substance; The accurate title, decide; Put in the 10ml measuring bottle; Add dissolve with methanol and be diluted to and contain the mixed solution of getting saikoside a 0.4mg, saikoside b 0.5mg, saikoside c 3mg, saikoside d 0.5mg among every 1ml, shake up, promptly get;
The preparation of need testing solution: get the about 0.6g of these article, the accurate title, decide, and puts in the 20ml measuring bottle, adds methyl alcohol to scale, and ultrasonic 10 minutes, filter, get subsequent filtrate, promptly get;
Accurate respectively object of reference solution and each 20ul of need testing solution of drawing of determination method injects liquid chromatograph, measures, and writes down 55 minutes chromatogram, promptly gets characteristic spectrum, sees Fig. 2,3.
Should present 4 characteristic peaks in the test sample characteristic spectrum; With the corresponding peak of object of reference be the S peak; Calculate each characteristic peak and the corresponding peak-to-peak relative retention time of contrast characteristic spectrum; 3 peaks should be identical with corresponding object of reference peak retention time respectively therein, its relative retention time should setting ± 5% within; Setting is: 17.133 (peaks 1), 20.950 (peaks 2), 21.167 (peaks 3), 26.467 (peaks 4).
(5) foundation of TLC thin layer chromatic graph
According to " appendix a VI of Chinese pharmacopoeia version in 2010 B test.
It is that reference substance and extract contrast that this thin layer is differentiated with saikoside abcd, adopts the contrast of saikoside abcd mixed solution and extract simultaneously, takes the dual contrast of thin layer like this, favorable reproducibility, and specificity is strong.The selection of thin layer condition is according to " appendix a VI of Chinese pharmacopoeia version in 2010 B test.
Claims (2)
1. a radix bupleuri standardized extract differentiates and content assaying method that its operation steps is following:
(1) gets Bupleurum Chinese and be ground into powder, add 60% ethanol, transfer pH=8, soak into 8h with stabilizing agent; Under 40 ℃ of conditions of temperature, ultrasonic Extraction 3 times each 40 minutes, adds 8 times of amount ethanol at every turn; Transfer pH=8 with stabilizing agent, filter merging filtrate; Reclaim ethanol to there not being the alcohol flavor, under 60 ℃, be concentrated into relative density 1.10 spray dryings or micro-wave vacuum, promptly get;
(2) differentiate:
The preparation of need testing solution: get bupleurum extract 0.6g, add methyl alcohol 10ml, sonicated 2min filters, as need testing solution;
The preparation of saikoside reference substance solution: get saikoside a reference substance, saikoside d reference substance, saikoside b reference substance, saikoside c reference substance, add methyl alcohol and process the mixed solution that every 1ml contains 0.4mg, as reference substance solution;
" each 10 μ l of above-mentioned solution are drawn in an appendix VI of Chinese pharmacopoeia version in 2010 B test, put respectively on same silica gel g thin-layer plate according to thin-layered chromatography; With ethyl acetate, ethanol, water=8: 2: 1 was developping agent, launched, and took out; Dry; It is clear that spray is heated to the spot colour developing with 40% sulfuric acid solution of 2% pair of dimethylbenzaldehyde at 60 ℃, under daylight and ultraviolet lamp 365n m, inspects respectively, in the test sample chromatogram; Be on the reference substance chromatogram relevant position, show the spot or the fluorescence spot of same color;
(3) saikoside assay: " appendix VID of Chinese pharmacopoeia version in 2010 measures according to high performance liquid chromatography; Chromatographic condition and system suitability test are carried out according to assay under the radix bupleuri item;
The preparation of reference substance solution: get saikoside a reference substance, saikoside d reference substance, saikoside c reference substance, saikoside b reference substance are an amount of, accurately claim surely, add methyl alcohol and process the solution that every 1ml contains each 0.4mg of saikoside, shake up, and promptly get;
The preparation of need testing solution: get the about 0.6g of these article powder, the accurate title, decide, and puts in the 20ml measuring bottle, adds methyl alcohol to scale, and ultrasonic 10 minutes, filter, get subsequent filtrate, promptly get;
Determination method: accurate respectively reference substance solution 10ul and the need testing solution 10ul of drawing, inject liquid chromatograph, measure, promptly get;
These article are pressed dry product and are calculated, and contain saikoside a (C
42H
68O
13), saikoside d (C
42H
68O
13) total amount of saikoside c, saikoside b must not be less than 1.3%.
2. differentiate and content assaying method that according to the described bupleurum extract of claim 1 its operation steps comprises as follows: " characteristic spectrum ", " characteristic peak coincide collection of illustrative plates " are measured: photograph high performance liquid chromatography " appendix VID mensuration of Chinese pharmacopoeia version in 2010;
Chromatographic condition and system suitability test: with the octadecylsilane chemically bonded silica is filling agent; With the acetonitrile is mobile phase A, is Mobile phase B with water, and the regulation in the according to the form below is carried out gradient elution; The detection wavelength is 210nm, and number of theoretical plate calculates by saikoside a peak should be not less than 10000;
The preparation of object of reference solution: it is an amount of to get saikoside a reference substance, saikoside b reference substance, saikoside c reference substance, saikoside d reference substance; The accurate title, decide; Put in the 10ml measuring bottle; Add dissolve with methanol and be diluted to and contain the mixed solution of getting saikoside a 0.4mg, saikoside b 0.5mg, saikoside c 3mg, saikoside d 0.5mg among every 1ml, shake up, promptly get;
The preparation of need testing solution: get the about 0.6g of these article, the accurate title, decide, and puts in the 20ml measuring bottle, adds methyl alcohol to scale, and ultrasonic 10 minutes, filter, get subsequent filtrate, promptly get;
The characteristic peak collection of illustrative plates determination method of coincideing: accurate respectively object of reference solution and each 10ul of need testing solution of drawing, mix and inject liquid chromatograph, measure, write down 55 minutes chromatogram, promptly get the identical collection of illustrative plates of characteristic peak;
The characteristic spectrum determination method: accurate respectively object of reference solution and each 20ul of need testing solution of drawing, inject liquid chromatograph, measure, write down 55 minutes chromatogram, promptly get characteristic spectrum;
Should present 4 characteristic peaks in the test sample characteristic spectrum; With the corresponding peak of object of reference be the S peak; Calculate each characteristic peak and the corresponding peak-to-peak relative retention time of contrast characteristic spectrum; 3 peaks should be identical with corresponding object of reference peak retention time respectively therein, its relative retention time should setting ± 5% within; Setting is: 17.133 (peaks 1), 20.950 (peaks 2), 21.167 (peaks 3), 26.467 (peaks 4).
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014198105A1 (en) * | 2013-06-13 | 2014-12-18 | Chen Haoda | Calibrating method of control extract and application thereof |
| CN108445139A (en) * | 2018-03-26 | 2018-08-24 | 河南省洛正药业有限责任公司 | A kind of discrimination method of Chinese medicine preparation that treating synovitis |
| CN109030674A (en) * | 2018-07-05 | 2018-12-18 | 广州卡马生物科技有限公司 | A kind of radix bupleuri reference extract and its preparation method and application |
| CN109596746A (en) * | 2018-12-28 | 2019-04-09 | 成都普思生物科技股份有限公司 | A kind of application of new content assaying method in the control of Radix Bupleuri quality |
| CN111103379A (en) * | 2019-12-11 | 2020-05-05 | 山西大学 | Method for identifying different varieties of radix bupleuri |
| CN111289668A (en) * | 2020-03-30 | 2020-06-16 | 山东省中医药研究院 | Method for identifying bupleurum chinense and other bupleurum chinense varieties by using HPLC fingerprint |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014198105A1 (en) * | 2013-06-13 | 2014-12-18 | Chen Haoda | Calibrating method of control extract and application thereof |
| CN108445139A (en) * | 2018-03-26 | 2018-08-24 | 河南省洛正药业有限责任公司 | A kind of discrimination method of Chinese medicine preparation that treating synovitis |
| CN109030674A (en) * | 2018-07-05 | 2018-12-18 | 广州卡马生物科技有限公司 | A kind of radix bupleuri reference extract and its preparation method and application |
| CN109596746A (en) * | 2018-12-28 | 2019-04-09 | 成都普思生物科技股份有限公司 | A kind of application of new content assaying method in the control of Radix Bupleuri quality |
| CN111103379A (en) * | 2019-12-11 | 2020-05-05 | 山西大学 | Method for identifying different varieties of radix bupleuri |
| CN111103379B (en) * | 2019-12-11 | 2021-05-14 | 山西大学 | Method for identifying different varieties of radix bupleuri |
| CN111289668A (en) * | 2020-03-30 | 2020-06-16 | 山东省中医药研究院 | Method for identifying bupleurum chinense and other bupleurum chinense varieties by using HPLC fingerprint |
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Application publication date: 20120711 |