CN102565223A - System for detecting riboflavin in health care product through ion chromatography, on-line photochemical derivation and fluorescence analysis - Google Patents
System for detecting riboflavin in health care product through ion chromatography, on-line photochemical derivation and fluorescence analysis Download PDFInfo
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- CN102565223A CN102565223A CN2011104422259A CN201110442225A CN102565223A CN 102565223 A CN102565223 A CN 102565223A CN 2011104422259 A CN2011104422259 A CN 2011104422259A CN 201110442225 A CN201110442225 A CN 201110442225A CN 102565223 A CN102565223 A CN 102565223A
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Abstract
The invention relates to an ion chromatography, on-line photochemical derivation and fluorescence detection system, in particular to a system for detecting riboflavin in a health care product through ion chromatography, on-line photochemical derivation and fluorescence analysis. The system comprises a liquid chromatogram pump, a sample injection valve, an ion chromatographic column, a home-made photochemical reaction device, a power supply and a fluorescence detector. Polar organic compounds without or with weak fluorescence intensity and homologous compounds thereof are separated on the ion chromatographic column, and the separated components are oxidized into strong fluorescence substances on line in the home-made photochemical reaction device and are detected and analyzed by the fluorescence detector; and moreover, signals are recorded and processed by a chromatographic work station. The system has a single-pump single-flow path structure and an on-line oxidation mode, and the method is rapid and sensitive and can be used for detecting and analyzing certain specific organic matters in organisms and medicines.
Description
Technical field
The present invention relates to the using system that a kind of chemical apparatuses is analyzed, particularly relate to the chromatography of ions-online photochemistry that detects lactochrome and derive-system of fluorescence analysis.
Background technology
Lactochrome (Riboflavin) is vitamin B2, by a kind of water-soluble B family vitamin that isoalloxazine base and ribityl are formed, is the composition of flavin adenine dinucleotide (FAD) (FAD) and 2 kinds of coenzyme of FMN (FMN).It is the essential nutriment that humans and animals is kept body normal configuration and function; Extensively be present in the various histocytes; Participate in brain energy and metabolism with the coenzyme form, comprise direct oxidation phosphorylation and the metabolism such as synthetic and oxidation of fatty acid in the tricarboxylic acid cycle.Can occur angular stomatitis, canker sore, external genital organs or mucocutaneous pathology during Vitamin B2 deficiency, and Vitamin B2 deficiency is also closely related with the generation of anaemia, some tumour.Lactochrome separates with high performance liquid chromatography usually, analyzes as detecting device with mass spectrum, and this kind method institute use instrument costs an arm and a leg, and operating cost is high, should not promote operation.Lactochrome has hypofluorescence character, through deriving, can make it become the lumiflavin with hyperfluorescence character, detects with fluorescence method, and common deriving method has chemical derivatization.Chemical derivatization need consume chemical reagent, wastes time and energy.It is with the novel fluorescence analysis method of photon as derivative reagent that photochemistry is derived.Because the adding of photon can not introduced impurity in reaction system, can diluted sample yet, avoided adding chemically derived many unfavorable factors.Up to now, do not see report with chromatography of ions-online photochemistry post-column derivation-fluorescence spectrometry lactochrome.
Summary of the invention
The present invention is just to the weak point of prior art, provide a kind of chromatography of ions-online photochemistry derive-system of fluorescence analysis is in order to measuring lactochrome, this system simplification the detection technique of lactochrome, shortened analysis time.
Concrete technical scheme of the present invention is following:
The present invention is that a kind of chromatography of ions-online photochemistry is derived-fluorescence detecting system; The outlet of anion chromatographic exchange column is joined with self-control photochemical reactor inlet; The inlet of photochemical reactor outlet and fluorescence detector joins; The outlet of fluorescence detector is connected with the waste liquid cylinder, and fluorescence signal is connected with workstation with computing machine through signal wire.
The present invention is through ion chromatographic separation, and some polar organic matters (lactochrome) that do not have or have faint fluorescence intensity can be the hyperfluorescence material by online photochemical oxidation, and need not carry out complicated off-line and online chemical oxidization method.
The material that the present invention also can be applied to not have fluorescence or faint photoluminescent property is analyzed or is improved sensitivity and improve detectability.
The present invention has following advantage and effect:
The present invention separates with the chromatography of ions, through the photochemistry post-column derivation, detects with fluorescence detector; Compare with chemically derived, saved a pump and chemical reagent, total system only needs a pump, and a single line stream is simple in structure, has simplified the detection technique of lactochrome, has shortened analysis time.
Description of drawings
Fig. 1 is the lactochrome schematic diagram that photochemistry is derived under alkali condition;
Fig. 2 derives for chromatography of ions-photochemistry-process flow diagram of system of fluorescence analysis;
Among Fig. 2,1-moving phase, 2-liquid chromatography pump, 3-sampling valve, 4-anion chromatographic exchange column, 5-photochemical reactor, 6-teflon pipe, 7-fluorescence detector, 8-computing machine and workstation, 9-waste liquid cylinder;
Fig. 3 is the standard model (lactochrome) and actual sample (multidimensional sheet) the fluorescence signal peak of chromatography of ions-photochemistry post-column derivation-system of fluorescence analysis;
Among the figure, 1-lactochrome (10.0mgL
-1), 2-actual sample (multidimensional sheet).
Embodiment
Through specific embodiment technical scheme of the present invention is further specified below:
As shown in Figure 1: lactochrome is derived through photochemistry under alkali condition, generates lumiflavin.The lactochrome molecule is not a planar structure, forms the lumiflavin of planar molecule through the back of deriving.
As shown in Figure 2: moving phase 1 is carried from liquid chromatography pump 2; Analytic sample is from quantifying ring 3 quantitative sample injections; Component after anion chromatographic exchange column 4 separates is at homemade photochemical reactor 5, and 6 are oxidized to the hyperfluorescence material in the teflon pipe of braiding, and oxidation product is transported to fluorescence detector 7 by homemade photochemical reactor outlet and detects; Signal is by computing machine and workstation 8 records and processing, and the tail washings that the autofluorescence detecting device is discharged is through sewer pipe to waste liquid cylinder 9.
With the ion chromatograph of U.S. Dai An company (Dionex), and Ion Pac AG11-HC guard column (4mm * 50mm), Ion PacAS11-HC analytical column (4mm * 250mm), with 40mmolL
-1NaOH is a moving phase, and flow rate of mobile phase is 1.0mL/min, and sample size is 25 μ L.Homemade photochemical reactor, fluorescent tube is 18w, and emission wavelength is mainly 254nm, and the pipeline of deriving is that the internal diameter of 20m braiding is the 0.5mm teflon pipe.RF-535 fluorescence detector (day island proper Tianjin company produces), excitation wavelength is 335m, emission wavelength is 510nm.Under this experiment condition, derive-system of fluorescence analysis with chromatography of ions of the present invention-online photochemistry, can carry out compartment analysis to each component in the multidimensional sheet.Wherein lactochrome detects and is limited to 0.300 μ gmL
-1
As shown in Figure 3: 1 is the lactochrome chromatogram of 10ppm, and 2 are actual sample (multidimensional sheet) chromatogram after treatment.
Claims (3)
1. chromatography of ions-online photochemistry is derived-fluorescence detecting system; It is characterized in that; Anion chromatographic exchange column (4) outlet is joined with self-control photochemical reactor (5) inlet; The inlet of photochemical reactor (5) outlet and fluorescence detector (7) joins, and the outlet of fluorescence detector (7) is connected with waste liquid cylinder (9), and fluorescence signal is connected with workstation (8) with computing machine through signal wire.
2. a chromatography of ions-photochemistry is derived-purposes of fluorescence detecting system; It is characterized in that; Pass through ion chromatographic separation; Some polar organic matters (lactochrome) that do not have or have faint fluorescence intensity can be the hyperfluorescence material by online photochemical oxidation, and need not carry out complicated off-line and online chemical oxidization method.
3. purposes according to claim 2 is characterized in that, the material that this method also can be applied to not have fluorescence or faint photoluminescent property is analyzed or improved sensitivity and improve detectability.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104422259A CN102565223A (en) | 2011-12-26 | 2011-12-26 | System for detecting riboflavin in health care product through ion chromatography, on-line photochemical derivation and fluorescence analysis |
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| CN2011104422259A CN102565223A (en) | 2011-12-26 | 2011-12-26 | System for detecting riboflavin in health care product through ion chromatography, on-line photochemical derivation and fluorescence analysis |
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| CN102565223A true CN102565223A (en) | 2012-07-11 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102565222A (en) * | 2011-12-26 | 2012-07-11 | 浙江大学 | Ion chromatography-online photochemical derivatization-fluorescence analysis system for measuring carbamazepine content in tablets |
| CN103115981A (en) * | 2013-01-29 | 2013-05-22 | 广东中烟工业有限责任公司 | Quantitative detection method for riboflavin in tobacco |
| CN106290275A (en) * | 2016-07-30 | 2017-01-04 | 郑州科技学院 | Molecular fluorescence difference mark-on is utilized to measure egg yolk, VB2the method of riboflavin in tablet |
| CN114894949A (en) * | 2022-04-12 | 2022-08-12 | 北京清谱科技有限公司 | Lipid fine structure analysis process and control processing system |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102565222A (en) * | 2011-12-26 | 2012-07-11 | 浙江大学 | Ion chromatography-online photochemical derivatization-fluorescence analysis system for measuring carbamazepine content in tablets |
-
2011
- 2011-12-26 CN CN2011104422259A patent/CN102565223A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102565222A (en) * | 2011-12-26 | 2012-07-11 | 浙江大学 | Ion chromatography-online photochemical derivatization-fluorescence analysis system for measuring carbamazepine content in tablets |
Non-Patent Citations (3)
| Title |
|---|
| K. CALLMER,ET AL: "Separation and determination of vitamin B1, B2, B6 and nicotinamide in commercial vitamin preparations using high performance cation-exchange chromatography", 《CHROMATOGRAPHIA》, vol. 7, no. 11, 30 November 1974 (1974-11-30), pages 644 - 650 * |
| 任一平等: "柱后光化学衍生荧光检测高效液相色谱法测定食品中的维生素B1", 《分析化学》, vol. 28, no. 5, 31 May 2000 (2000-05-31), pages 1 * |
| 刘珺等: "高效液相色谱分离-在线光化学衍生/荧光光谱法测定5种水溶性维生素的研究", 《分析测试学报》, vol. 30, no. 10, 31 October 2011 (2011-10-31) * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102565222A (en) * | 2011-12-26 | 2012-07-11 | 浙江大学 | Ion chromatography-online photochemical derivatization-fluorescence analysis system for measuring carbamazepine content in tablets |
| CN103115981A (en) * | 2013-01-29 | 2013-05-22 | 广东中烟工业有限责任公司 | Quantitative detection method for riboflavin in tobacco |
| CN103115981B (en) * | 2013-01-29 | 2014-06-04 | 广东中烟工业有限责任公司 | Quantitative detection method for riboflavin in tobacco |
| CN106290275A (en) * | 2016-07-30 | 2017-01-04 | 郑州科技学院 | Molecular fluorescence difference mark-on is utilized to measure egg yolk, VB2the method of riboflavin in tablet |
| CN106290275B (en) * | 2016-07-30 | 2019-01-22 | 郑州科技学院 | Yolk, VB are measured using molecular fluorescence difference mark-on2The method of riboflavin in tablet |
| CN114894949A (en) * | 2022-04-12 | 2022-08-12 | 北京清谱科技有限公司 | Lipid fine structure analysis process and control processing system |
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Application publication date: 20120711 |