CN102565220A - Iron chromatography-ultraviolet detection method for determining content of Ramipril medicine - Google Patents
Iron chromatography-ultraviolet detection method for determining content of Ramipril medicine Download PDFInfo
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- CN102565220A CN102565220A CN2011104413936A CN201110441393A CN102565220A CN 102565220 A CN102565220 A CN 102565220A CN 2011104413936 A CN2011104413936 A CN 2011104413936A CN 201110441393 A CN201110441393 A CN 201110441393A CN 102565220 A CN102565220 A CN 102565220A
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- ramipril
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- leacheate
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Abstract
The invention provides a novel detection method for Ramipril, which jointly adopts an iron chromatography cation exchange system and an ultraviolet detector, selects an appropriate separation condition and then selects an optimal ultraviolet detection wave length according to substance behavior difference reserved on a chromatography column, can detects Ramipril in separation mode under high sensitivity, and can be used for detection of the content of Ramipril in the Ramipril medicine, human body fluid pharmacokinetic research and the like.
Description
Technical field
The present invention relates to the using system that a kind of chemical apparatuses is analyzed, particularly relate to the chromatography of ions-ultraviolet detection system that detects Ramipril.
Background technology
It is a kind of in the drug for hypertension that Ramipril belongs to angiotensin converting enzyme inhibitor (ACEI); Commonly used have captopril, enalapril, fosinopril, Benazepril, Cilazapril, Perindopril, Ramipril and a Trandolapril etc.; These medicines have certain spinoff more, nervous system spinoffs such as digestive tract reaction, headache, dizziness such as that nonspecific spinoff has is nauseating, vomiting.Common analytical approach mainly detects with HPLC-ultraviolet detection and HPLC-MS.Need regulate the moving phase pH value when separating about 3 with HPLC, and silicagel column can not strong alkali-acid resistance and the Ramipril structure in contain amino, under sour environment, exist with the organic cations form, be more suitable for using ion chromatographic separation.Up to now, do not see the report of chromatography of ions-uv detection method mensuration Ramipril medicament contg.
Summary of the invention
The present invention to the weak point of prior art, provides a kind of chromatography of ions-cation exchange-UV-detector system just, can separation detection Ramipril medicine under the isocratic condition, and favorable reproducibility, highly sensitive.Concrete technical scheme of the present invention is following:
The present invention is the method that a kind of chromatography of ions-uv detection method is measured the Ramipril medicament contg, earlier it is transformed into ionic compound through acid moving phase, separates through cation exchange column again, selects suitable wavelengths to detect.
The present invention utilizes the effect of Ramipril sample acid in leacheate to form down kation, is transported to guard column, cation exchange column with leacheate by pump behind the six-way valve sample introduction, passes through the separating spectrum that UV-detector obtains sample again.The separating mechanism of the method is a cation exchange, selects suitable separation conditions such as leacheate that it is well separated on cation exchange column, and selects best detection wavelength, thereby accurately measure the content of Ramipril.
Ramipril of the present invention can be able to separation detection effectively through chromatography of ions-cation exchange column, and pre-treatment is simple, and analysis time is short, highly sensitive.
The present invention also can be applied to fields such as pharmacy, pharmacokinetic study.
Beneficial effect of the present invention is following:
Do not report both at home and abroad the method for this kind detection Ramipril as yet, this method can be widely used in fields such as bio-pharmaceuticals, pharmacokinetic study, drug safety, can separation detection Ramipril medicine under the isocratic condition, and favorable reproducibility, highly sensitive.
Description of drawings
Fig. 1 is the works synoptic diagram that chromatography of ions of the present invention-uv detection method is measured the Ramipril medicament contg;
Among the figure, the 1-leacheate; The 2-pump; The 3-six-way valve; The 4-protection is lived; The 5-cation exchange column; The 6-UV-detector; The 7-sample;
Fig. 2 is the separating spectrums of Ramipril standard items on cation exchange column;
Among the figure, the 1-Ramipril;
Fig. 3 is the separating spectrum of Ramipril sheet composition on cation exchange column;
Among the figure, the 1-Ramipril.
Embodiment
Through specific embodiment technical scheme of the present invention is further specified below:
Chromatography of ions-uv detection method is measured the content of Ramipril in the Ramipril sheet
1, the preparation of standard solution: accurately take by weighing Ramipril standard specimen 10mg, put adding 50% acetonitrile in the 10ml volumetric flask, after the concussion dissolving, constant volume is to scale;
2, the making of typical curve: the method preparation variable concentrations standard solution that adopts stepwise dilution;
3, sample pre-treatments: remove the tablet dressing, grind into powder accurately adds the dissolving of 50% acetonitrile after the weighing, and ultrasonic 15 minutes to be cooled to room temperature, quantitatively to scale mark.After being diluted to suitable concn, 0.45 μ m membrane filtration;
4, the selection of leacheate: test different leacheate proportionings, finally confirm as: 35% acetonitrile+12%50mM Loprazolam+53% water can well separate the Ramipril medicine;
5, sample size 7 μ L, UV scanning confirm that the optimal detection wavelength is 215nm, in 0.5 μ g/ml-25 μ g/ml scope internal linear relation good (r=0.9999), detect and are limited to 0.151 μ g/ml.
As shown in Figure 1, this method is to utilize the effect of Ramipril sample acid in leacheate 1 to form down kation, is transported to guard column 4, cation exchange column 5 with leacheate 1 by pump 2 behind six-way valve 3 sample introductions, passes through the separating spectrum that UV-detector 6 obtains sample again.The separating mechanism of the method is a cation exchange, selects suitable leacheate 1 condition such as separation such as grade that it is well separated on cation exchange column 5, and selects best detection wavelength, thereby accurately measure the content of Ramipril.This method can be widely used in fields such as bio-pharmaceuticals, pharmacokinetic study, food security.
As shown in Figure 21 is 5ppm Ramipril standard items separating spectrums under the moving phase condition of 35% acetonitrile+12%50mM Loprazolam+53% water.
Be illustrated in figure 3 as through grinding, extract, the Ramipril sample after the dilution is separating spectrum under the moving phase condition of 35% acetonitrile+12%50mM Loprazolam+53% water, and 1 is Ramipril, and appearance time is suitable, and the peak type is good, more helps accurately quantitatively.
Claims (4)
1. the method for chromatography of ions-uv detection method mensuration Ramipril medicament contg is characterized in that, earlier it is transformed into ionic compound through peracidity leacheate (1), separates through cation exchange column (5) again, selects suitable wavelengths to detect.
2. method according to claim 1; It is characterized in that; The effect that utilizes the acid in leacheate (1) of Ramipril sample is the formation kation down, is transported to guard column (4), cation exchange column (5) with leacheate (1) by pump (2) behind six-way valve (3) sample introduction, passes through the separating spectrum that UV-detector (6) obtains sample again; This separating mechanism is a cation exchange; Select suitable leacheate separation conditions such as (1) that it is gone up well at cation exchange column (5) and separate, and select best detection wavelength, thereby accurately measure the content of Ramipril.
3. the purposes of the method for chromatography of ions-uv detection method mensuration Ramipril medicament contg is characterized in that Ramipril can be able to separation detection effectively through chromatography of ions-cation exchange column, and pre-treatment is simple, and analysis time is short, highly sensitive.
4. purposes according to claim 3 is characterized in that this method also can be applied to fields such as pharmacy, pharmacokinetic study.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104413936A CN102565220A (en) | 2011-12-26 | 2011-12-26 | Iron chromatography-ultraviolet detection method for determining content of Ramipril medicine |
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| CN2011104413936A CN102565220A (en) | 2011-12-26 | 2011-12-26 | Iron chromatography-ultraviolet detection method for determining content of Ramipril medicine |
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| CN102565220A true CN102565220A (en) | 2012-07-11 |
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| CN2011104413936A Pending CN102565220A (en) | 2011-12-26 | 2011-12-26 | Iron chromatography-ultraviolet detection method for determining content of Ramipril medicine |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101275934A (en) * | 2008-04-16 | 2008-10-01 | 浙江树人大学 | Ion chromatogram-alkali liquor inhibition ultraviolet detecting amines compounds |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101275934A (en) * | 2008-04-16 | 2008-10-01 | 浙江树人大学 | Ion chromatogram-alkali liquor inhibition ultraviolet detecting amines compounds |
Non-Patent Citations (1)
| Title |
|---|
| 林立 等: "离子色谱-紫外检测测定液态奶中的三聚氰胺", 《食品科学》, vol. 30, no. 14, 31 December 2009 (2009-12-31), pages 240 - 242 * |
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Application publication date: 20120711 |