CN102533630A - Wuyiencin spore powder and production technology thereof - Google Patents
Wuyiencin spore powder and production technology thereof Download PDFInfo
- Publication number
- CN102533630A CN102533630A CN2011103521100A CN201110352110A CN102533630A CN 102533630 A CN102533630 A CN 102533630A CN 2011103521100 A CN2011103521100 A CN 2011103521100A CN 201110352110 A CN201110352110 A CN 201110352110A CN 102533630 A CN102533630 A CN 102533630A
- Authority
- CN
- China
- Prior art keywords
- wuyiencin
- spore powder
- spore
- wuyi
- gram
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses wuyiencin spore powder and a production technology thereof. The production technology comprises liquid culture, solid culture, drying, crushing and separation so as to obtain the wuyiencin spore powder, wherein spore number of the wuyiencin spore powder is greater than or equal to 50.0*10<8>, the water content is less than or equal to 8%, the pH value is 4.0-7.0, and the resolution ratio of the effective components is less than or equal to 5%. In the invention, through the expansion culture of liquid phase before solid phase, the spore powder content of the obtained wuyiencin spore powder is greater than 50*10<8>/g, thus the stress resistance of the spores is strong, thereby being favorable for storage and transportation; the wuyiencin spore powder can be used in various modes by directly spraying powder or spraying after being prepared into a liquid preparation, thereby being convenient to use; and the wuyiencin spore powder is particularly suitable for preventing diseases of woods and fruit trees, and has an obvious prevention effect.
Description
Technical field
The present invention relates to a kind of Wuyiencin, specifically a kind of Wuyiencin spore powder and production technique thereof utilize the Wuyiencin spore to be applied to the prevention and control of plant diseases, pest control of plant as biological pesticide, belong to field of biological pesticide.
Background technology
Traditional sterilant major part is a chemical pesticide, and wherein some biological pesticide is to utilize submerged fermentation, and the metabolic substd that useful microbe is produced has the effect (being also referred to as biochemicals) of bactericidal.Traditional chemical pesticide sterilant, its germ-resistant main effective constituent is difficult to decompose at occurring in nature, and residual period, is long, and is harmful to biology, though do not advocate, but still be the main products in the plant protection technology.
The most of toxicity of biochemical pesticide is lower, and Environmental compatibility is bigger, is technology and the product of advocating now.And biological pesticide is also found the weakness of some problems in promoting the use of: the one, and production cost is high, and the use cost of bringing is high; The 2nd, biochemical metabolite has breakthrough development than the technological difficulty that valency improves; The 3rd, the biochemical products metabolite also can impel the disease worm to develop immunity to drugs in preventing and treating the process of crop pest, and must strengthen working concentration, has influenced actual popularization; The 4th, water aqua type biochemical pesticide principal market sprays on protection ground booth vegetable etc., irritates root, and having strengthened temperature has reduced effect, and it is also influenced to prevent and treat disease.Various environmental differences is also brought the difference of result of use.
Wuyiencin is the biological pesticide product with preventing disease production-increasing effect of Chinese Academy of Agricultural Sciences development, its derive from not streptomyces hygroscopicus Wuyi mutation (
Streptomyces anhygroscopicus var.wuyiensis) a kind of microbiotic (1984: agricultural antibiotic BO-10 produces the evaluation of bacterium for mikrobe journal, 24:401-402) of producing; Be mainly used in control farm crop fungus disease; Multiple fungal diseases such as the Powdery Mildew of plant, graw mold of tomato, leaf muld of tomato, scab of cucumber are had good control effect, and its staple is the nucleoside antibiotics with cytidine skeleton, to the person poultry safety; Therefore, in the ecological agriculture and pollution-free food production, have broad application prospects.
Summary of the invention
In order to overcome the limitation of above-mentioned biological pesticide; The invention provides a kind of Wuyiencin spore powder and production technique thereof; Utilize deep fermentation and the process method that is connected two stages of solid fermentation to reach to turn out the high-load Wuyi bacterium spore powder biological pesticide of 5,000,000,000/gram Wuyi bacterium spore; Adopt suitability for industrialized production, be beneficial to the biological pesticide enlargement of scale.
Technical scheme of the present invention is:
A kind of production technique of Wuyiencin spore powder may further comprise the steps:
(1) liquid culture: the 5-10% that the Wuyi bacterial classification is pressed the liquid nutrient medium quality inoculates, and under 28 ℃ of constant temperature, utilizes cradle vibrate fermentation 2-3 days, obtains containing the mycelial liquid in Wuyi;
(2) solid culture: by weight being that 1 ︰ 3-4 mixes, the solid fermentation cultivation is 3-5 days under temperature 28-30 ℃, the condition of humidity 60-80% with the Wuyi mycelium that obtains of fermentation in above-mentioned steps (1) liquid fermentation medium and solid fermentation substratum;
(3) drying: the cultivation material that contains abundant spore behind above-mentioned steps (2) solid fermentation is carried out drying, and 45 ℃ of drying temperatures time 10-30 minute, make material moisture to being lower than 8%;
(4) pulverize separation: the dried dry thing that contains a large amount of Wuyis bacterium spore of above-mentioned steps (3) is pulverized, utilized 80-100 purpose screen cloth then, behind separation stock culture material and the Wuyi mycelium, obtain Wuyi bacterium spore powder.
Before above-mentioned steps (1) inoculation; Wuyi bacterium bacterium is carried out activation; The bacterial classification of preservation state is put into suitable activation medium; Enlarged culturing is in order to obtain pure and strong Wuyi bacterial classification, generally to need the 2-3 rejuvenation process in generation, letting bacterial classification adapt to gradually and make production culture of strains environment step by step.
Wherein, the consisting of of described liquid fermentation medium: yam 150-200 gram, glucose 15-20 gram, potassium primary phosphate 1-2 gram, 1000 milliliters of sal epsom 0.5-1 grams, yeast extract paste 0.5 gram, peptone 0.5 gram, sodium-chlor 0.5 gram, water, pH value 6.5.
The massfraction of described solid fermentation substratum consists of: soybean cake powder 20-30%, Semen Maydis powder 5-20%, wheat bran 5-10%, potassium primary phosphate 0.08-0.2%, trimagnesium phosphate 0.2%, lime carbonate 0.3%, water complementary.
The thickness of the solid fermentation substratum of said step (2) is 6-8 centimetre, when media surface is covered with mycelia, all turns over and makes for one time culture evenly accept air and moisture up and down in the culturing process, so that Wuyi bacterium spore enlarges increment in media surface.
The drying plant of said step (3) is a belt vacuum and low temperature continuous drier.
Adopt 5,000,000,000 of the spore up of the spore goods that the inventive method obtains/more than the gram; Biphasic fermentation is cultivated the Wuyi spore powder; At first cultivate the purity that has guaranteed the Wuyi bacterial classification, avoided interference and the Wuyi bacterium spore " growth of geometry level multiple " of other assorted bacterium in liquid phase; After changing solid-state cultivation again over to, under good gaseity, facilitate Wuyi mycelia and spore to grow up more rapidly, more favourable recovery primary characteristic and growing way.Because therefore the strong stress resistance of spore is beneficial to preservation, transportation and several different methods and uses the particularly disease control of forest, fruit tree.
Spore quantity>=50.0 * 10 of the spore goods that employing the inventive method obtains
8, water cut≤8%, pH value=4.0-7.0, effective constituent rate of decomposition≤5%, heat storage stability is qualified.
Wuyi bacterium spore powder is long volution, general 5-12 circle spiral, and spore is circular or oval, and size is even, and spore surface is barbed, and the living bacterium powder of gas is pearl or grey on the PDA substratum, and the bacterium powder is yellow in the base.
Find with test after deliberation; Utilize Wuyiencin to produce bacterium as biocontrol microorganisms, can directly prevent and treat soil-borne disease of crop, for example preventing and treating the verticillium of eggplant, the blight of cotton etc. all has obvious effects; Be superior to that other agricultural chemicals have more efficiently, the characteristics of wide spectrum, low toxicity, environmental safety
The diseases prevention principle of Wuyiencin spore powder is that live body is secreted microbiotic behind diseased plant blade face, root growth, and the eubiosis that crop surface is kept plant growth is competed and parasitized to niche.Do not produce resistance and residual toxicity after chemical pesticide uses.
Be improved through spore reproduction ability after the prescription of adjustment substratum, the liquid phase of particularly taking to optimize cultivates that to add method that the native place spreads cultivation be Wuyi bacterium solid phase body fermentation efficiency 500,000,000/g spore of living, and brings up to more than 5,000,000,000/g.The Wuyi spore powder biological pesticide that utilizes this product to process, its diseases prevention principle are niche competition, parasitic and secretion microbiotic.Because the used culture medium prescription of the present invention is different with the conventional art prescription, particularly the keeping mode of solid-state cultivation is improved, and it is very competent that the Wuyi spore produces spore, and the Product industrialization cost is low.Field test proves that Wuyi bacterium spore powder can adopt and spray, dresses seed, topdresses, spreads manuer in holes etc.Effectively the antimicrobial substance component content is high in its viable bacteria metabolite behind crop body surface or root soil parasitism for its viable bacteria, and to rob the mass point positive with germ.Form simultaneously and stimulate the mechanism of making the spontaneous degeneration-resistant disease and pest of deposits yields, be consistent with " plant health is advocated theory " that international academic community was advocated in recent years.
The invention has the advantages that: utilize first liquid phase again the Wuyi bacterium spore powder content that obtains of solid phase enlarged culturing reach more than 5,000,000,000/gram; Because of the strong stress resistance of spore, therefore, be more conducive to preserve, transport; And method of use is versatile and flexible; Can adopt pulvis directly to spray, also can be made into liquid preparation and spray, easy to use; Not only raw material environmental protection simultaneously; And make preceding, also environmental protection when making the neutralization use, reach the requirement of low-carbon (LC) and green, have very high social benefit and environmental benefit; Can be widely used in the multiple diseases control of crops such as vegetables, fruit tree, forestry, control effect is remarkable.
Embodiment
Below the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein only is used for explanation and explains the present invention, and be not used in qualification the present invention.
Embodiment 1
A kind of production technique of Wuyiencin spore powder may further comprise the steps:
1, with bacterium inclined-plane, the Wuyi seed after the activation, be 8% to be linked in the liquid fermentation medium by mass percentage, keep 28 ℃ of temperature, the shaking table concussion is cultivated, and ferments 3 days.
Wherein, consisting of of used liquid fermentation medium: yam 180 grams, glucose 16 grams, potassium primary phosphate 1.5 grams, sal epsom 1 gram, yeast extract paste 0.5 gram, peptone 0.5 gram, sodium-chlor 0.5 gram, 1000 milliliters in water, pH value 6.5.
2, with cultured Wuyi bacterium mycelium in the liquid fermentation medium and solid fermentation substratum by weight being that 1 ︰ 3 mixes; (to scatter after agglomerating be standard to hold) stirs; Under 28 ℃, humidity 60% condition, carry out the solid phase fermentation; The thickness of substratum is 6 centimetres under the solid state fermentation conditions, when watering in time keeps humidity to be covered with the bacterium powder on the material surface all upset make solid-state culture material evenly accept temperature and moisture, air, enlarged culturing effect.Cultivate the gas that produced in 4 days near natural form and give birth to Wuyi bacterium spore.
Wherein, the massfraction of solid fermentation substratum consists of: soybean cake powder 20%, Semen Maydis powder 20%, wheat bran 5%, potassium primary phosphate 0.08%, trimagnesium phosphate 0.2%, lime carbonate 0.3%, water complementary.
3, the cultivation material that contains abundant spore behind the above-mentioned solid fermentation is utilized PLG series continuous vacuum dryer (changzhou city development drying plant ltd; Specification type1500/12 type power 11kw) carry out drying, the adjustment Vacuumdrier is at 45 ℃ of whole fermentation materials of oven dry.
4 pulverize the solid state fermentation material after the above-mentioned oven dry.
5 utilize mechanical vibration to separate obtains Wuyi bacterium spore powder.
Adopt the ZD6*5 vibrating screen classifier to make spore and mycelium and cultivation feed separation, obtain the Wuyi spore powder of 5,000,000,000 of content/more than the gram through microscopy and biological assay.
Wuyi of the present invention bacterium spore powder controlling index is following:
Simultaneous test: protection ground leaf muld of tomato control test
Leaf muld of tomato is the Deuteromycotina disease; Protection ground cultivation hot and humid is multiple disease; Adopt Wuyi bacterium spore powder biotechnological formulation (the spore powder powder that present embodiment makes with fill the actual content that obtains after auxiliary material zeyssatite mixes 200,000,000/preparation that restrains; Auxiliary material zeyssatite can dilute spore powder, lets spore powder attached on the stopping composition simultaneously, and it is easy to use to be convenient to agricultural.) with derosal the same terms of 800 times of liquid under, foliage-spray is weekly, uses effect such as following table altogether 3 times:
The result: compare with contrast, these article have antagonism, nutrient competition effect to leaf mold, Wuyi bacterium spore to leaf mold can discern, contact, penetrate, parasitic usefulness, controlled the generation of leaf muld of tomato.
Embodiment 2
A kind of production technique of Wuyiencin spore powder may further comprise the steps:
1, with bacterium inclined-plane, the Wuyi seed after the activation, be 5% to be linked in the liquid fermentation medium by mass percentage, keep 28 ℃ of temperature, the shaking table concussion is cultivated, and ferments 2 days.
Wherein, consisting of of used liquid fermentation medium: yam 200 grams, glucose 15 grams, potassium primary phosphate 2 grams, sal epsom 0.5 gram, yeast extract paste 0.5 gram, peptone 0.5 gram, sodium-chlor 0.5 gram, 1000 milliliters in water, pH value 6.5.
2, with cultured Wuyi bacterium mycelium in the liquid fermentation medium and solid fermentation substratum by weight being that 1 ︰ 3 mixes; (to scatter after agglomerating be standard to hold) stirs; Under 30 ℃, humidity 70% condition, carry out the solid phase fermentation; The thickness of substratum is 8 centimetres under the solid state fermentation conditions, when watering in time keeps humidity to be covered with the bacterium powder on the material surface all upset make solid-state culture material evenly accept temperature and moisture, air, enlarged culturing effect.Cultivate the gas that produced in 5 days near natural form and give birth to Wuyi bacterium spore.
Wherein, the massfraction of solid fermentation substratum consists of: soybean cake powder 22%, Semen Maydis powder 10%, wheat bran 10%, potassium primary phosphate 0.2%, trimagnesium phosphate 0.2%, lime carbonate 0.3%, water complementary.
3, the solid phase of a large amount of mycelia of above-mentioned generation, spore is cultivated material and carry out drying, the adjustment Vacuumdrier is at 45 ℃ of whole fermentation materials of oven dry.
4 pulverize the solid state fermentation material after the above-mentioned oven dry.
5 utilize mechanical vibration to separate obtains Wuyi bacterium spore powder.
Wuyi of the present invention bacterium spore powder controlling index is following:
Simultaneous test: the preventive and therapeutic effect test of cotton wilt
Method is with embodiment 1
The result: compare with contrast, these article to cotton wilt have antagonism, penetrate, parasitization, controlled the generation of cotton wilt.
Embodiment 3
A kind of production technique of Wuyiencin spore powder may further comprise the steps:
1, with bacterium inclined-plane, the Wuyi seed after the activation, be 7% to be linked in the liquid fermentation medium by mass percentage, keep 28 ℃ of temperature, the shaking table concussion is cultivated, and ferments 3 days.
Wherein, consisting of of used liquid fermentation medium: yam 200 grams, glucose 15 grams, potassium primary phosphate 1 gram, sal epsom 1 gram, yeast extract paste 0.5 gram, peptone 0.5 gram, sodium-chlor 0.5 gram, 1000 milliliters in water, pH value 7.5.
2, with cultured Wuyi bacterium mycelium in the liquid fermentation medium and solid fermentation substratum by weight being that 1 ︰ 4 mixes, stir (to scatter after agglomerating be standard to hold),
30 ℃, carry out the solid phase fermentation under humidity 80% condition, the thickness of substratum is 8 centimetres under the solid state fermentation conditions, when watering in time keeps humidity to be covered with the bacterium powder on the material surface all upset make solid-state culture material evenly accept temperature and moisture, air, enlarged culturing effect.Cultivate the gas that produced in 5 days near natural form and give birth to Wuyi bacterium spore.
Wherein, the massfraction of solid fermentation substratum consists of: soybean cake powder 30%, Semen Maydis powder 5%, wheat bran 10%, potassium primary phosphate 0.2%, trimagnesium phosphate 0.2%, lime carbonate 0.3%, water complementary.
3, the solid phase of a large amount of mycelia of above-mentioned generation, spore is cultivated material and carry out drying, the adjustment Vacuumdrier is at 45 ℃ of whole fermentation materials of oven dry.
4 pulverize the solid state fermentation material after the above-mentioned oven dry.
5 utilize mechanical vibration to separate obtains Wuyi bacterium spore powder.
Wuyi of the present invention bacterium spore powder controlling index is following:
Simultaneous test: the preventive and therapeutic effect test of protection Viola grypoceras A. Gray gray mold
Method is with embodiment 1
The result: compare with contrast, these article to gray mold of cucumber have antagonism, penetrate, parasitization, controlled the generation of gray mold of cucumber.
Embodiment 4
A kind of production technique of Wuyiencin spore powder may further comprise the steps:
1, with bacterium inclined-plane, the Wuyi seed after the activation, be 10% to be linked in the liquid fermentation medium by mass percentage, keep 28 ℃ of temperature, the shaking table concussion is cultivated, and ferments 2 days.
Wherein, consisting of of used liquid fermentation medium: yam 150 grams, glucose 20 grams, potassium primary phosphate 1 gram, sal epsom 1 gram, yeast extract paste 0.5 gram, peptone 0.5 gram, sodium-chlor 0.5 gram, 1000 milliliters in water, pH value 6.5.
2, with cultured Wuyi bacterium mycelium in the liquid fermentation medium and solid fermentation substratum by weight being that 1 ︰ 3 mixes; (to scatter after agglomerating be standard to hold) stirs; Under 30 ℃, humidity 70% condition, carry out the solid phase fermentation; The thickness of substratum is 7 centimetres under the solid state fermentation conditions, when watering in time keeps humidity to be covered with the bacterium powder on the material surface all upset make solid-state culture material evenly accept temperature and moisture, air, enlarged culturing effect.Cultivate the gas that produced in 3 days near natural form and give birth to Wuyi bacterium spore.
The solid fermentation substratum consist of soybean cake powder 20%, Semen Maydis powder 10%, wheat bran 10%, potassium primary phosphate 0.2%, trimagnesium phosphate 0.2%, lime carbonate 0.3%, water complementary.
3, the solid phase of a large amount of mycelia of above-mentioned generation, spore is cultivated material and carry out drying, the adjustment Vacuumdrier is at 45 ℃ of whole fermentation materials of oven dry.
4 pulverize the solid state fermentation material after the above-mentioned oven dry.
5 utilize mechanical vibration to separate obtains Wuyi bacterium spore powder.
Wuyi of the present invention bacterium spore powder controlling index is following:
Simultaneous test: the preventive and therapeutic effect test of protection Fruit of Common Pratia gray mold
Method is with embodiment 1
The result: compare with contrast, these article to grey mould of egg plant have antagonism, penetrate, parasitization, controlled the generation of grey mould of egg plant.
Among each embodiment of the present invention, used chemical industry material is the raw material commonly used in the production of this area, all can from market, obtain, and can not exert an influence for the production result; The equipment of in each operation, using all adopts conventional equipment used in the current production, does not have special feature.
What should explain at last is: the above is merely the preferred embodiments of the present invention; Be not limited to the present invention; Although the present invention has been carried out detailed explanation with reference to previous embodiment; For a person skilled in the art, it still can be made amendment to the technical scheme that aforementioned each embodiment put down in writing, and perhaps part technical characterictic wherein is equal to replacement.All within spirit of the present invention and principle, any modification of being done, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (7)
1. the production technique of a Wuyiencin spore powder is characterized in that, may further comprise the steps:
(1) liquid culture: the 5-10% that the Wuyi bacterial classification is pressed the liquid nutrient medium quality inoculates, and under 28 ℃ of constant temperature, utilizes cradle vibrate fermentation 2-3 days;
(2) solid culture: by weight being that 1 ︰ 3-4 mixes, the solid fermentation cultivation is 3-5 days under temperature 28-30 ℃, the condition of humidity 60-80% with the Wuyi mycelium that obtains of fermentation in above-mentioned steps (1) liquid fermentation medium and solid fermentation substratum;
(3) drying: the cultivation material behind above-mentioned steps (2) solid fermentation is carried out drying, and 45 ℃ of drying temperatures time 10-30 minute, make material moisture to being lower than 8%;
(4) pulverize separation: the dried dry thing that contains a large amount of Wuyis bacterium spore of above-mentioned steps (3) is pulverized, utilized 80-100 purpose screen cloth then, behind separation stock culture material and the Wuyi mycelium, obtain Wuyi bacterium spore powder.
2. the production technique of Wuyiencin spore powder according to claim 1 is characterized in that: before above-mentioned steps (1) inoculation, the bacterial classification of Wuyi bacterium is carried out activation.
3. the production technique of Wuyiencin spore powder according to claim 1; It is characterized in that: the thickness of the solid fermentation substratum of said step (2) is 6-8 centimetre; In the culturing process when media surface is covered with mycelia; All turn over and make for one time culture evenly accept air and moisture up and down, so that Wuyi bacterium spore enlarges increment in media surface.
4. the production technique of Wuyiencin spore powder according to claim 1; It is characterized in that: the consisting of of described liquid fermentation medium: yam 150-200 gram; Glucose 15-20 gram; Potassium primary phosphate 1-2 gram, 1000 milliliters of sal epsom 0.5-1 grams, yeast extract paste 0.5 gram, peptone 0.5 gram, sodium-chlor 0.5 gram, water, pH value 6.5.
5. the production technique of Wuyiencin spore powder according to claim 1; It is characterized in that: the massfraction of described solid fermentation substratum consists of: soybean cake powder 20-30%, Semen Maydis powder 5-20%, wheat bran 5-10%; Potassium primary phosphate 0.08-0.2%; Trimagnesium phosphate 0.2%, lime carbonate 0.3%, water complementary.
6. the production technique of Wuyiencin spore powder according to claim 1 is characterized in that: the drying plant of said step (3) is a belt vacuum and low temperature continuous drier.
7. a Wuyiencin spore powder that is prepared by the production technique of any described Wuyiencin spore powder among the claim 1-6 is characterized in that: spore quantity>=50.0 * 10 of described Wuyiencin spore powder
8, water cut≤8%, pH value=4.0-7.0, effective constituent rate of decomposition≤5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110352110 CN102533630B (en) | 2011-11-09 | 2011-11-09 | Wuyiencin spore powder and production technology thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110352110 CN102533630B (en) | 2011-11-09 | 2011-11-09 | Wuyiencin spore powder and production technology thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102533630A true CN102533630A (en) | 2012-07-04 |
| CN102533630B CN102533630B (en) | 2013-06-05 |
Family
ID=46341667
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110352110 Expired - Fee Related CN102533630B (en) | 2011-11-09 | 2011-11-09 | Wuyiencin spore powder and production technology thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102533630B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110800763A (en) * | 2019-10-18 | 2020-02-18 | 姚爽 | Biological pesticide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101050441A (en) * | 2007-03-30 | 2007-10-10 | 中国农业科学院植物保护研究所 | Heavy producing bacterial strain F64 for Wuyi bacterin, and fermentation process |
| CN101748171A (en) * | 2010-01-14 | 2010-06-23 | 潍坊万胜生物农药有限公司 | A kind of processing method of from Wuyiencin submerged fermentation liquid, extracting Wuyi bacterium oligosaccharides |
| CN101948766A (en) * | 2010-08-16 | 2011-01-19 | 潍坊万胜生物农药有限公司 | Process method for producing Wuyi biocontrol bacteria by utilizing waste residues generated by deeply fermenting Wuyiencin |
-
2011
- 2011-11-09 CN CN 201110352110 patent/CN102533630B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101050441A (en) * | 2007-03-30 | 2007-10-10 | 中国农业科学院植物保护研究所 | Heavy producing bacterial strain F64 for Wuyi bacterin, and fermentation process |
| CN101748171A (en) * | 2010-01-14 | 2010-06-23 | 潍坊万胜生物农药有限公司 | A kind of processing method of from Wuyiencin submerged fermentation liquid, extracting Wuyi bacterium oligosaccharides |
| CN101948766A (en) * | 2010-08-16 | 2011-01-19 | 潍坊万胜生物农药有限公司 | Process method for producing Wuyi biocontrol bacteria by utilizing waste residues generated by deeply fermenting Wuyiencin |
Non-Patent Citations (1)
| Title |
|---|
| 王立东等: "应用响应面法优化武夷菌素发酵培养基", 《西南师范大学学报(自然科学版)》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110800763A (en) * | 2019-10-18 | 2020-02-18 | 姚爽 | Biological pesticide |
| CN110800763B (en) * | 2019-10-18 | 2021-08-31 | 黑龙江森工农化有限公司 | Storage tank for biological pesticides |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102533630B (en) | 2013-06-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102964178B (en) | Composite microbial bactericide, and preparation method and application thereof | |
| US8518428B2 (en) | Antagonistic bacteria for controlling the Fusarium wilt of continuous cropping banana and their microbial organic fertilizer | |
| CN104498386B (en) | The preparation method and application of raw Bacillus amyloliquefaciens strain SZ23 and zymotic fluid in wild jujube | |
| CN101243801B (en) | Water dispersible powder of Paemobacillus polymyxa, preparation and application thereof | |
| CN107254427A (en) | A kind of Bei Laisi Bacillus strains JN5 and its application | |
| CN101875571B (en) | Method for preparing enhanced liquid microbial organic fertilizer | |
| CN108949614A (en) | A kind of microbial bacterial agent of Bei Laisi bacillus | |
| CN103820348A (en) | Plant growth-promoting bacteria and preparation method and application of fungicide | |
| CN105112343A (en) | Wheat straw decomposition agent and method for preparing same | |
| CN103194410B (en) | Paenibacillus mucilaginosus and method for producing compound microorganism bacterium agent by utilizing same | |
| CN106011022B (en) | A kind of rose yellow streptomycete solid fermentation culture medium and its preparation and fermentation process | |
| CN102286413B (en) | Preparation method of liquid fermentation medium for bacillus thuringiensis | |
| CN110387340B (en) | Lactobacillus plantarum L16 and application thereof in preventing and treating vegetable diseases | |
| CN101928673B (en) | Trichoderma harzianum | |
| CN102876587A (en) | Cordyceps militaris strain for producing cordycepin with high yield | |
| CN109574719A (en) | A kind of multifunctional composite microbe bacterial manure and its application | |
| CN104232538A (en) | Efficient potassium bacterium and application thereof | |
| CN103540556B (en) | Streptomyces lavendulae and application of Streptomyces lavendulae to preparation of algae microbial fertilizer | |
| CN111278966A (en) | Large-scale production of liquid and solid trichoderma products | |
| CN105237248A (en) | Grifola frondosa production culture medium and application thereof | |
| CN102986737A (en) | Compound preparation of nematode egg parasitical fungi and spore germination accelerating agent and application thereof | |
| CN105695342B (en) | Koning trichoderma bacterium TG-72 and its application in Aspergillus flavus biological control | |
| CN105638744A (en) | Preparation method of Brevibacillus brevis wettable powder | |
| CN112794746B (en) | Composite bacterial powder for Chinese yam and preparation method thereof, and composite bacterial fertilizer for Chinese yam and preparation method thereof | |
| CN106831082A (en) | Improve the planting material and its cultural method of clitocybe maxima yield and amino acid content |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130605 Termination date: 20211109 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |