CN102532216A - Method for extracting sophoricoside from sophora fruits - Google Patents
Method for extracting sophoricoside from sophora fruits Download PDFInfo
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- CN102532216A CN102532216A CN2010106038682A CN201010603868A CN102532216A CN 102532216 A CN102532216 A CN 102532216A CN 2010106038682 A CN2010106038682 A CN 2010106038682A CN 201010603868 A CN201010603868 A CN 201010603868A CN 102532216 A CN102532216 A CN 102532216A
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- fructus sophorae
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- 238000000034 method Methods 0.000 title claims abstract description 13
- 235000013399 edible fruits Nutrition 0.000 title abstract description 6
- 241000219784 Sophora Species 0.000 title abstract 3
- ISQRJFLLIDGZEP-CMWLGVBASA-N Sophoricoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(C=2C(C3=C(O)C=C(O)C=C3OC=2)=O)C=C1 ISQRJFLLIDGZEP-CMWLGVBASA-N 0.000 title abstract 2
- ISQRJFLLIDGZEP-KJRRRBQDSA-N Sophoricoside Natural products O([C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O1)c1ccc(C=2C(=O)c3c(O)cc(O)cc3OC=2)cc1 ISQRJFLLIDGZEP-KJRRRBQDSA-N 0.000 title abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 58
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 14
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000011347 resin Substances 0.000 claims abstract description 12
- 229920005989 resin Polymers 0.000 claims abstract description 12
- 229910021538 borax Inorganic materials 0.000 claims abstract description 10
- 235000010339 sodium tetraborate Nutrition 0.000 claims abstract description 10
- 239000012065 filter cake Substances 0.000 claims abstract description 9
- 238000010992 reflux Methods 0.000 claims abstract description 7
- 230000007935 neutral effect Effects 0.000 claims abstract description 3
- 229930182470 glycoside Natural products 0.000 claims description 27
- 150000002338 glycosides Chemical class 0.000 claims description 27
- 239000000284 extract Substances 0.000 claims description 16
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 12
- 238000010828 elution Methods 0.000 claims description 10
- 238000000605 extraction Methods 0.000 claims description 10
- 239000004328 sodium tetraborate Substances 0.000 claims description 9
- 238000002425 crystallisation Methods 0.000 claims description 7
- 230000008025 crystallization Effects 0.000 claims description 7
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 238000004090 dissolution Methods 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 238000010298 pulverizing process Methods 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 4
- 239000000287 crude extract Substances 0.000 claims description 4
- 238000001953 recrystallisation Methods 0.000 claims description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000001179 sorption measurement Methods 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 7
- 239000000047 product Substances 0.000 abstract description 6
- 238000001914 filtration Methods 0.000 abstract 2
- 239000003480 eluent Substances 0.000 abstract 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 abstract 1
- 238000011160 research Methods 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 244000046101 Sophora japonica Species 0.000 description 3
- 235000010586 Sophora japonica Nutrition 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 206010030113 Oedema Diseases 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 229930003944 flavone Natural products 0.000 description 2
- 150000002213 flavones Chemical class 0.000 description 2
- 235000011949 flavones Nutrition 0.000 description 2
- TZBJGXHYKVUXJN-UHFFFAOYSA-N genistein Natural products C1=CC(O)=CC=C1C1=COC2=CC(O)=CC(O)=C2C1=O TZBJGXHYKVUXJN-UHFFFAOYSA-N 0.000 description 2
- 235000006539 genistein Nutrition 0.000 description 2
- 229940045109 genistein Drugs 0.000 description 2
- ZCOLJUOHXJRHDI-CMWLGVBASA-N genistein 7-O-beta-D-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC(O)=C2C(=O)C(C=3C=CC(O)=CC=3)=COC2=C1 ZCOLJUOHXJRHDI-CMWLGVBASA-N 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- LKZDFKLGDGSGEO-UJECXLDQSA-N kaempferol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H]1[C@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=CC(O)=CC=2)=O)O[C@H](CO)[C@@H](O)[C@@H]1O LKZDFKLGDGSGEO-UJECXLDQSA-N 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 235000013824 polyphenols Nutrition 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- LKZDFKLGDGSGEO-UHFFFAOYSA-N 2',8-Di-Me ether,7-O-beta-D-glucuronoside-2',5,7,8-Tetrahydroxyflavone Natural products OC1C(O)C(O)C(CO)OC1OC1C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=CC(O)=CC=2)=O)OC(CO)C(O)C1O LKZDFKLGDGSGEO-UHFFFAOYSA-N 0.000 description 1
- ZWSNUPOSLDAWJS-QNDFHXLGSA-N 6,7-dihydroxy-3-[4-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyphenyl]chromen-4-one Chemical compound OC[C@H]1O[C@@H](Oc2ccc(cc2)-c2coc3cc(O)c(O)cc3c2=O)[C@H](O)[C@@H](O)[C@@H]1O ZWSNUPOSLDAWJS-QNDFHXLGSA-N 0.000 description 1
- 0 CC(C(C(C1O)O*)N=O)OC1Oc1ccc(C(COc2cc(O)cc(O)c22)C2=O)cc1 Chemical compound CC(C(C(C1O)O*)N=O)OC1Oc1ccc(C(COc2cc(O)cc(O)c22)C2=O)cc1 0.000 description 1
- 206010008909 Chronic Hepatitis Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 206010030247 Oestrogen deficiency Diseases 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 101710098398 Probable alanine aminotransferase, mitochondrial Proteins 0.000 description 1
- CWJHHOQFXOOROL-UHFFFAOYSA-N Sophorine+ Natural products C1CCCC2C(CNC(=O)CCCC(=O)OCCCC)CCCN21 CWJHHOQFXOOROL-UHFFFAOYSA-N 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- ANJTVLIZGCUXLD-DTWKUNHWSA-N cytisine Chemical compound C1NC[C@H]2CN3C(=O)C=CC=C3[C@@H]1C2 ANJTVLIZGCUXLD-DTWKUNHWSA-N 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000001076 estrogenic effect Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 229930182486 flavonoid glycoside Natural products 0.000 description 1
- 150000007955 flavonoid glycosides Chemical class 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- HVQAJTFOCKOKIN-UHFFFAOYSA-N flavonol Natural products O1C2=CC=CC=C2C(=O)C(O)=C1C1=CC=CC=C1 HVQAJTFOCKOKIN-UHFFFAOYSA-N 0.000 description 1
- 150000007946 flavonol Chemical class 0.000 description 1
- 235000011957 flavonols Nutrition 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 208000035861 hematochezia Diseases 0.000 description 1
- 208000014617 hemorrhoid Diseases 0.000 description 1
- 230000002439 hemostatic effect Effects 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 230000009245 menopause Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
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- 150000003648 triterpenes Chemical class 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention provides a method for extracting sophoricoside from sophora fruits, which is characterized by enabling sophora fruit medicinal materials to be smashed, adding 8-12 times amount of alkalinity thin alcohol liquid containing sodium borate to heat and reflux for extracting 2-3 times, adjusting extracting solution to be neutral, passing macroporous resin column, eluting by using ethanol water, enabling eluent to be concentrated to extract, adding defined amount ethyl acetate for dissolving, filtering, dissolving undissolved substance by using acetone, filtering, obtaining filter cakes to conduct backflow dissolving by using 70-90% ethanol, and crystallizing 2-4 times to obtain products. The products manufactured by the method are high in product contents, good in repeatability and large in handling capacity of macroporous resin and have industrialization significances.
Description
Technical field:
The invention belongs to the Separation of Natural Products field, especially relate to a kind of method of from the Fructus Sophorae, extracting Fructus Sophorae glycosides.
Background technology:
The Fructus Sophorae is China's conventional Chinese medicine material, the dry mature fruit of leguminous plants Chinese scholartree (Sophora japonica L.).The medicinal material commodity are claimed the Fructus Sophorae with fruit.It is stated from Eastern Han Dynasty's Shennong's Herbal as medicinal head, and quilt is classified as top grade, and fruit claims that Chinese scholartree is real, the promptly modern Fructus Sophorae, and later successive dynasties book on Chinese herbal medicine is all on the books.Fructus Sophorae bitter, cold in nature.Return liver, large intestine channel.Clearing away heat-fire, blood cooling and hemostatic functions are arranged.Be used for intestines heat and have blood in stool, hemorrhoid are swollen hemorrhage, the headache of liver heat, dizzy hot eyes.The staple of the Fructus Sophorae is compositions such as flavonoid, alkaloids, triterpenes, amino acid and carbohydrate, and wherein NOVASOY 400 and flavonol and glycoside component content thereof are higher.
Fructus Sophorae glycosides has another name called Sophoraflavonoloside, sophorine, and genistein-4 '-glucoside, white tip-like crystallization is insoluble in water, ethanol, is dissolved in hot ethanol slightly, is dissolved in pyridine, diluted alkaline, is insoluble to ETHYLE ACETATE, acetone.Structural formula:
Fructus Sophorae glycosides belongs to isoflavone glycoside, has stronger physiologically active.Modern pharmacological research shows that Fructus Sophorae glycosides is respond well to chronic hepatitis patient's glutamate pyruvate transaminase lowering, can also suppress the pawl oedema that is caused by Oleum Tiglii inductive dropsy of ear and X 5189 simultaneously.The document that Xu Yong etc. deliver " Fructus Sophorae glycosides and genistein are to the influence of the scleroblast biological characteristics " document the is clear and definite estrogen-like effects of Fructus Sophorae glycosides; Document shows that Fructus Sophorae glycosides can effectively stop the bone amount that causes because of estrogen deficiency to run off in vivo; The tissue morphology that keeps bone, Prevention and Treatment of Osteoporosis.Clinical study confirms that also Fructus Sophorae glycosides can be used for preventing and treating the symptom of women's osteosporosis after menopause, and therefore, Fructus Sophorae glycosides has fabulous application prospect and exploitation future.
More in the existing document to researchs such as genistein, Genistosides, and mainly be to rest on the assay aspect to the research of Fructus Sophorae glycosides.The Master's thesis of Liu Xiaohua " research of the Fructus Sophorae and " the Chinese pharmacopoeia version Fructus Sophorae in 2005 assay item standard revision "; The method that this paper openly extracts Fructus Sophorae glycosides is an ethanol ultrasonic extraction, concentrates the first water removal of impurities in back, uses ether defatting again; Obtain product with the N recrystallization at last; Though this method operation is easy, the toxic organic solvent consumption is big, and product content is not high.
Summary of the invention:
The technical problem that the present invention will solve provides a kind of method of from the Fructus Sophorae, extracting Fructus Sophorae glycosides, and this technology makes the product content height, quality is good.
The objective of the invention is to realize through following technical scheme:
A kind of method of from the Fructus Sophorae, extracting Fructus Sophorae glycosides is characterized in that comprising following step:
1) extract: with Fructus Sophorae raw material pulverizing, adds after 8～12 times of amount rare pure liquid of alkalescence and borax mix, heating and extracting 2～3 times, extracting solution adds acid and is adjusted to neutrality, must Fructus Sophorae glycosides crude extract;
2) macroporous resin adsorption: above-mentioned crude extract is crossed macroporous resin adsorption, and washing to sugared reaction negative is used 50～70% ethanolic soln wash-out more earlier, collects elutriant and is concentrated into medicinal extract;
3) removal of impurities: above-mentioned medicinal extract is used an amount of acetic acid ethyl dissolution, and insolubles is used acetone solution again, filters, and gets filter cake;
4) recrystallization: with above-mentioned filter cake with 60～80% alcohol reflux crystallization 2～4 times.
The consumption of borax is 1%～2% of the Fructus Sophorae in the said step 1), and the rare pure liquid pH of alkalescence is controlled at 8～9 (15～30% ethanol), and extraction time is 2～4 hours, extracts temperature and is controlled at 60～80 ℃, and being used to regulate neutral acid can be hydrochloric acid or sulfuric acid.
The model of the macroporous resin said step 2) can adopt a kind of among AB-8, HPD450 or the D101, and the ethanolic soln consumption is 3～6BV, and elution flow rate is controlled to be 0.5～2BV/h.
ETHYLE ACETATE in the said step 3) is 2～5 times of medicinal extract amount, and the acetone consumption is 2～4 times of amounts of insolubles.
The present invention adopts the low pure liquid of alkalescence to extract, because under alkaline condition, the leaching effect of flavones ingredient is better, adds borax and extracts, and can protect phenolic hydroxyl group, prevents that neighbour's two phenolic hydroxyl groups in the Fructus Sophorae glycosides from oxidation taking place under alkaline condition; Adopt oil-soluble impuritieses such as other flavones of acetoneand ethyl acetate flush away, flavonoid glycoside; Utilize the dissolubility difference of Fructus Sophorae glycosides in cold and hot ethanol to carry out the recrystallization operation at last, obtain highly purified product, simple to operate, preparation amount is big.
To combine embodiment to further specify the present invention below, but the scope that the present invention requires to protect is not limited to following embodiment.
Embodiment:
Embodiment 1:
With Fructus Sophorae pulverizing medicinal materials, take by weighing 1kg, add pH9.0 alkalescence rare pure liquid (20% ethanol) that 10L contains the 20g borax, heating and refluxing extraction 2 times; Each 2.5 hours, united extraction liquid added hydrochloric acid and is adjusted to neutrality, extracting solution is added in the AB-8 type macroporous resin column adsorbs again; Washing to sugared reaction negative is followed 65% ethanol elution with 5BV earlier, and elution flow rate is 0.5BV/h, collects elutriant; Be concentrated into medicinal extract, add the 120ml acetic acid ethyl dissolution, filter, insolubles is used the acetone solution of 100ml again; Filter, filter cake gets Fructus Sophorae glycosides 37.1g, content 98.3% 3 times with 70% dissolve with ethanol crystallization.
Embodiment 2:
With Fructus Sophorae pulverizing medicinal materials, take by weighing 1kg, add pH9.0 alkalescence rare pure liquid (15% ethanol) that 8L contains the 18g borax, heating and refluxing extraction 3 times; Each 2 hours, united extraction liquid added hydrochloric acid and is adjusted to neutrality, extracting solution is added in the HPD450 type macroporous resin column adsorbs again; Washing to sugared reaction negative is followed 70% ethanol elution with 6BV earlier, and elution flow rate is 2BV/h, collects elutriant; Be concentrated into medicinal extract, add the 100ml acetic acid ethyl dissolution, filter, insolubles is used the acetone solution of 80ml again; Filter, filter cake gets Fructus Sophorae glycosides 33.6g, content 98.7% 4 times with 80% dissolve with ethanol crystallization.
Embodiment 3:
With Fructus Sophorae pulverizing medicinal materials, take by weighing 2kg, add pH8.5 alkalescence rare pure liquid (25% ethanol) that 22L contains the 28g borax, heating and refluxing extraction 3 times; Each 3 hours, united extraction liquid added hydrochloric acid and is adjusted to neutrality, extracting solution is added in the HPD450 type macroporous resin column adsorbs again; Washing to sugared reaction negative is followed 50% ethanol elution with 4BV earlier, and elution flow rate is 1.5BV/h, collects elutriant; Be concentrated into medicinal extract, add the 250ml acetic acid ethyl dissolution, filter, insolubles is used the acetone solution of 180ml again; Filter, filter cake gets Fructus Sophorae glycosides 70.3g, content 97.9% 2 times with 70% dissolve with ethanol crystallization.
Embodiment 4:
With Fructus Sophorae pulverizing medicinal materials, take by weighing 5kg, add pH8 alkalescence rare pure liquid (28% ethanol) that 60L contains the 50g borax, heating and refluxing extraction 3 times; Each 4 hours, united extraction liquid added hydrochloric acid and is adjusted to neutrality, extracting solution is added in the D101 type macroporous resin column adsorbs again; Washing to sugared reaction negative is followed 70% ethanol elution with 3BV earlier, and the collection elution flow rate is 1BV/h, elutriant; Be concentrated into medicinal extract, add the 600ml acetic acid ethyl dissolution, filter, insolubles is used the acetone solution of 500ml again; Filter, filter cake gets Fructus Sophorae glycosides 138.3g, content 98.1% 4 times with 80% dissolve with ethanol crystallization.
Claims (4)
1. method of from the Fructus Sophorae, extracting Fructus Sophorae glycosides is characterized in that comprising following step:
1) extract: with Fructus Sophorae raw material pulverizing, adds after 8～12 times of amount rare pure liquid of alkalescence and borax mix, heating and extracting 2～3 times, extracting solution adds acid and is adjusted to neutrality, must Fructus Sophorae glycosides crude extract;
2) macroporous resin adsorption: above-mentioned crude extract is crossed macroporous resin column absorption, and washing to sugared reaction negative is used 50～70% ethanolic soln wash-out more earlier, collects elutriant and is concentrated into medicinal extract;
3) removal of impurities: above-mentioned medicinal extract is used an amount of acetic acid ethyl dissolution, and insolubles is used acetone solution again, filters, and gets filter cake;
4) recrystallization: with above-mentioned filter cake with 60～80% alcohol reflux crystallization 2～4 times.
2. the method for from the Fructus Sophorae, extracting Fructus Sophorae glycosides as claimed in claim 1; The consumption that it is characterized in that borax in the said step 1) is 1%～2% of the Fructus Sophorae; The rare pure liquid pH of alkalescence is controlled at 8～9 (15～30% ethanol); Extraction time is 2～4 hours, extracts temperature and is controlled at 60～80 ℃, and being used to regulate neutral acid can be hydrochloric acid or sulfuric acid.
3. the method for from the Fructus Sophorae, extracting Fructus Sophorae glycosides as claimed in claim 1; It is characterized in that said step 2) in the model of macroporous resin can adopt a kind of among AB-8, HPD450 or the D101; The ethanolic soln consumption is 3～6BV, and elution flow rate is controlled to be 0.5～2BV/h.
4. the method for from the Fructus Sophorae, extracting Fructus Sophorae glycosides as claimed in claim 1 is characterized in that ETHYLE ACETATE in the said step 3) is 2～5 times of medicinal extract amount, and the acetone consumption is 2～4 times of amounts of insolubles.
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| CN2010106038682A CN102532216A (en) | 2010-12-24 | 2010-12-24 | Method for extracting sophoricoside from sophora fruits |
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| CN2010106038682A CN102532216A (en) | 2010-12-24 | 2010-12-24 | Method for extracting sophoricoside from sophora fruits |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103724381A (en) * | 2012-10-15 | 2014-04-16 | 天津药物研究院 | Preparation method of robinin-7-O-rhamnose glucoside and application thereof |
| CN108383885A (en) * | 2018-05-02 | 2018-08-10 | 孙波 | From the technique and its purposes in anti-inflammatory for extracting Sophoricoside in the Fructus Sophorae |
| CN113880889A (en) * | 2021-11-15 | 2022-01-04 | 西安久中生物科技有限公司 | Method for extracting sophoricoside, genistein and kaempferol from sophora fruit |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103724381A (en) * | 2012-10-15 | 2014-04-16 | 天津药物研究院 | Preparation method of robinin-7-O-rhamnose glucoside and application thereof |
| CN108383885A (en) * | 2018-05-02 | 2018-08-10 | 孙波 | From the technique and its purposes in anti-inflammatory for extracting Sophoricoside in the Fructus Sophorae |
| CN108383885B (en) * | 2018-05-02 | 2021-07-23 | 孙波 | The process of extracting sophoroside from Sophora japonica and its use in anti-inflammatory |
| CN113880889A (en) * | 2021-11-15 | 2022-01-04 | 西安久中生物科技有限公司 | Method for extracting sophoricoside, genistein and kaempferol from sophora fruit |
| CN113880889B (en) * | 2021-11-15 | 2023-12-29 | 西安久中生物科技有限公司 | Method for extracting sophoricoside, genistein and kaempferol from sophora fruit |
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