CN102532177A - Rapid detection reagent kit for benzoyl peroxide - Google Patents
Rapid detection reagent kit for benzoyl peroxide Download PDFInfo
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- CN102532177A CN102532177A CN2011104360464A CN201110436046A CN102532177A CN 102532177 A CN102532177 A CN 102532177A CN 2011104360464 A CN2011104360464 A CN 2011104360464A CN 201110436046 A CN201110436046 A CN 201110436046A CN 102532177 A CN102532177 A CN 102532177A
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Abstract
The invention discloses a rapid detection reagent kit for benzoyl peroxide, which comprises reagent stock solution 1 and reagent stock solution 2, wherein the reagent stock solution 1 is buffer solution, and the reagent stock solution 2 is phenyl borate solution containing resorufin fluorophore. The rapid detection reagent kit for the benzoyl peroxide has the advantages of being simple and convenient to operate, low in cost, rapid, efficient, sensitive and the like, is easy to popularize and apply, and has huge application prospects in the fields of food, medicine and the like.
Description
Technical field
The present invention relates to a kind of Lucidol quick detection kit.
Background technology
Lucidol (Benzoyl Peroxide is called for short BPO or BP) has been widely used in the bleaching of whole meal flour as whitening agent.Its ultimate principle is that Lucidol can make in the wheat-flour conjugated double bond of carrotenoid oxidized and destroy, thereby the color and luster of wheat-flour is improved and improves.Yet excessive interpolation Lucidol has not only influenced the mouthfeel of flour product, also produces the degradation production of a large amount of Lucidols simultaneously, is unfavorable for HUMAN HEALTH.From on May 1st, 2011, Ministry of Health of the People's Republic of China forbade in flour production, adding Lucidol.
The method of present various detection Lucidol content mainly contains HPLC, chemoluminescence and electrochemical methods etc.These method sample pre-treatments are loaded down with trivial details consuming time, and cost is high, is difficult for promoting the use of.Therefore, be necessary to set up the novel method of the sensitive rapid detection Lucidol of a kind of ability content, for basic unit's quality testing department provides technical support.Fluorescence analysis method not only has sensitive can realize characteristics such as visual detection fast, and, cost low receive generally simple because of pre-treatment paid attention to.But there be limited evidence currently of has fluorescent method to detect the report of Lucidol content.
Summary of the invention
The purpose of this invention is to provide a kind of Lucidol quick detection kit.
Phenyl-boron dihydroxide ester cpds provided by the invention, its structural formula is suc as formula shown in the I
Formula I.
The method of compound shown in the said formula I of preparation provided by the invention; Comprise the steps: under the condition that catalyzer exists; 7-hydroxyl Phenazoxine ketone sodium salt and 2-bromomethyl benzene boric acid pinacol ester mixing are reacted, and reaction finishes and obtains phenyl-boron dihydroxide ester cpds shown in the said formula I.
The equation of this reaction is following:
Formula I
In the aforesaid method, said catalyzer is selected from least a in organic bases and the mineral alkali; Said organic bases is selected from least a in triethylamine and the pyridine; Said mineral alkali is selected from least a in salt of wormwood, sodium hydroxide, yellow soda ash and the sodium hydrogencarbonate; The molar ratio of said 7-hydroxyl Phenazoxine ketone sodium salt, 2-bromomethyl benzene boric acid pinacol ester and catalyzer is 1: 0.5～5: 0.5～5, preferred 1: 1: 1 or 1: 4: 4 or 1: 1-4: 1-4; In the said reactions step, temperature is 0 ℃～60 ℃, and preferred 30 ℃, the time is 1～24 hour; Said being reflected in the organic solvent carried out; Said organic solvent is selected from N, at least a in dinethylformamide (DMF), triethylamine, THF and the acetonitrile.The consumption of organic solvent is as the criterion with complete solubilizing reaction thing, need not to limit especially its consumption.
Lucidol quick detection kit provided by the invention comprises reagent storing solution 1 and reagent storing solution 2; Wherein, said reagent storing solution 1 is a damping fluid; Said reagent storing solution 2 is the solution of compound shown in the aforementioned formula I.
In the said reagent storing solution 1 of this test kit, said damping fluid is that the pH value is the phosphate buffered saline buffer of 6～9 organic solvent, and said phosphoric acid salt is selected from Na
2HPO
4, NaH
2PO
4And KH
2PO
4In at least a; Said organic solvent is selected from methyl alcohol, ethanol, DMSO 99.8MIN., THF, acetonitrile and 1, at least a in the 4-dioxane; In the said damping fluid, said phosphatic concentration is 0.01～0.5M, is specially 0.02M; The concentration expressed in percentage by volume of said organic solvent in said damping fluid is 1%～60%, is specially 10%.
In the said reagent storing solution 2, the concentration of the solution of compound shown in the said formula I is 1-100 μ M, is specially 3-10 μ M; Solvent is selected from acetonitrile, methyl alcohol, ethanol, 1, and at least a in 4-dioxane and the THF is specially acetonitrile.
Above-mentioned said Lucidol quick detection kit also can only be made up of with reagent storing solution 2 said reagent storing solution 1.
The application of the Lucidol quick detection kit that the invention described above provides in detecting Lucidol content also belongs to protection scope of the present invention.
The method of said detection Lucidol content comprises the steps:
1) production standard curve:
A) with 550nm as excitation wavelength, the solution of measuring the Lucidol standard substance of a series of different concns is the fluorescence intensity at 585nm place at emission wavelength, is designated as F; And to measure reagent blank be the fluorescence intensity at 585nm place at emission wavelength, is designated as F
0, be X-coordinate with the concentration C of Lucidol, fluorescence intensity changing value Δ F is an ordinate zou, the drawing standard curve;
Wherein, Δ F=F-F
0The solution of the Lucidol standard substance of said a series of different concns is by the standard inventory solution mixing of reagent storing solution 1, reagent storing solution 2 and Lucidol in the arbitrary said Lucidol detection kit of claim 5-8 and water and alcohol dilution and get;
2) content of Lucidol in the detection testing sample:
The said Lucidol standard substance of said step 1) are replaced with said testing sample; Detecting said testing sample according to the said method of said step 1) is the fluorescence intensity at 585nm place at emission wavelength; Be designated as F '; With the said step 1) gained of said F ' substitution typical curve, obtain the concentration of Lucidol in the said testing sample, and then obtain the content of Lucidol in the said testing sample.
In the above-mentioned detection method, in the solution of the Lucidol standard substance of said a series of different concns, the concentration of said Lucidol is followed successively by 0,0.5,2,4,6,8,10,12,14,16,18,20,22,24,26 and 30 μ M;
The volume of the solution of the Lucidol standard substance of said a series of different concns is 3mL;
In the standard inventory solution of said Lucidol, solvent is an ethanol; The concentration of the standard inventory solution of said Lucidol is 1mM;
The reagent storing solution 1 in the said Lucidol detection kit and the volume ratio of said reagent storing solution 2 are 2.5mL: 9 μ L;
The corresponding equation of linear regression of the said typical curve of said step 1) is Δ F=56.2 * C+39.1, and wherein, C is the concentration of said Lucidol, and unit is μ g/mL.
Lucidol detection kit provided by the invention has following characteristics:
1) reagent storing solution 2 is little band orange, and does not have fluorescence; With then can show red-purple and send intensive fluorescence after the Lucidol reaction.
2) speed of response is fast, can develop the color in 15 minutes.
3) highly sensitive, Lucidol concentration can observe with the naked eye tangible red-purple and produce when >=1 μ M, and need not instrument and just can do semiquantitative determination.
4) compare with existing similar reagents box, except that highly sensitive, also have simple to operate, reaction temperature and advantage.
5) this coupling reaction only takes place under the condition that Lucidol exists, and other common inorganic salt (inorganic salt that comprise strong oxidizing property), carbohydrate, amino acid, VITAMINs do not produce interference.
6) easy to use, can directly be used for the detection of flour sample and gel antiseptic-germicide sample Lucidol.
7) have long fluorescent emission wavelength (585nm), so also available fluorescent spectrometry detects, limit of determination can reach >=23nM.Therefore, this test kit is a kind of excellent property, Lucidol test set easy to use, in food, medicine and other fields, great application prospect is arranged.
Description of drawings
Fig. 1 is the photo of the Lucidol solution reaction of test kit and different concns.
Fig. 2 is the concentration of Lucidol and the typical curve between the fluorescence intensity changing value.
Embodiment
Employed experimental technique is ordinary method like no specified otherwise among the following embodiment.
Used material, reagent etc. like no specified otherwise, all can obtain from commercial sources among the following embodiment.
Contain the preparation of the phenyl-boron dihydroxide ester cpds of resorufin fluorophore shown in embodiment 1, the formula I
7-hydroxyl Phenazoxine ketone sodium salt (1mmol) is dissolved among the DMF (10mL), adds 2-bromomethyl benzene boric acid pinacol ester (1mmol), catalyzer carbonic acid potassium (1mmol) in reaction solution, and 30 ℃ of reactions 1 hour.Reaction finishes, through the cooling after, the concentrating under reduced pressure solvent gets thick product, with n-hexane/ethyl acetate (2: 1, v/v) make elutriant, column chromatography purification of crude product obtains product 250mg.
The structural characterization data results of this product is following:
1H NMR (300MHz, CDCl
3) δ (ppm): 7.92 (d, 1H, J=7.3Hz), 7.74 (d, 1H, J=8.9Hz), 7.48 (m, 3H), 7.39 (m, 1H), 7.05 (m, 1H), 6.98 (d, 1H, J=2.5Hz), 6.91 (d, 1H, J=9.8Hz), 6.46 (s, 1H), 5.48 (s, 2H), 1.29 (s, 12H);
13C NMR (75MHz, CDCl
3) δ (ppm): 186.3,163.4,149.9,145.7,145.3,141.5,136.4,134.7,134.1,131.5,131.4,128.4,128.0,127.8,114.6,106.6,100.9,83.9,70.6,24.9.ESI-MS:m/z 430.4 [M+H]
+, 452.3 [M+Na]
+. ultimate analysis: calcd.for C
25H
24BNO
5(%): C 69.95, and H 5.64, and N 3.26; Found:C 69.21, and H 5.64, N 3.35.
By on can know that this product structure is correct, for containing the phenyl-boron dihydroxide ester cpds of resorufin fluorophore shown in the formula I.
Contain the preparation of the phenyl-boron dihydroxide ester cpds of resorufin fluorophore shown in embodiment 2, the formula I
7-hydroxyl Phenazoxine ketone sodium salt (1mmol) is dissolved among the DMF (10mL), adds 2-bromomethyl benzene boric acid pinacol ester (4mmol), catalyzer carbonic acid potassium (4mmol) in reaction solution, and 0～5 ℃ of reaction 24 hours.Reaction finishes, through the cooling after, the concentrating under reduced pressure solvent gets thick product, with n-hexane/ethyl acetate (2: 1, v/v) make elutriant, column chromatography purification of crude product obtains product 200mg.
The structural characterization data results of this product is following:
1H NMR (300MHz, CDCl
3) δ (ppm): 7.92 (d, 1H, J=7.3Hz), 7.74 (d, 1H, J=8.9Hz), 7.48 (m, 3H), 7.39 (m, 1H), 7.05 (m, 1H), 6.98 (d, 1H, J=2.5Hz), 6.91 (d, 1H, J=9.8Hz), 6.46 (s, 1H), 5.48 (s, 2H), 1.29 (s, 12H);
13C NMR (75MHz, CDCl
3) δ (ppm): 186.3,163.4,149.9,145.7,145.3,141.5,136.4,134.7,134.1,131.5,131.4,128.4,128.0,127.8,114.6,106.6,100.9,83.9,70.6,24.9.ESI-MS:m/z 430.4 [M+H]
+, 452.3 [M+Na]
+. ultimate analysis: calcd.for C
25H
24BNO
5(%): C 69.95, and H 5.64, and N 3.26; Found:C 69.21, and H 5.64, N 3.35.
By on can know that this product structure is correct, for containing the phenyl-boron dihydroxide ester cpds of resorufin fluorophore shown in the formula I.
Embodiment 3, with the Lucidol concentration in the test kit semiquantitative determination sample
With the Lucidol concentration in the test kit semiquantitative determination sample, measure according to following steps:
1) takes by weighing the 242mg Lucidol, be dissolved in the 100mL ethanol, be made into the Lucidol standard inventory solution of 10mM.
2) in sample bottle; Add 2.5mL reagent storing solution 1 and 9 μ L reagent storing solutions 2 successively; Add the Lucidol standard inventory solution of the proper volume that step 1) prepares again, water and alcohol dilution are respectively the ultimate density of Lucidol to 3mL: (a) 0 μ M; (b) 1 μ M; (c) 10 μ M; (d) 30 μ M; (e) 60 μ M; (f) 100 μ M shake up, and leave standstill 15min, obtain a series of development criteria, and respectively shown in the a-f among Fig. 1, by a-f, the color of solution is added gradually by the color of solvent itself and is deep to rediance.
Wherein, reagent storing solution 1 is 7.4 alcoholic acid Na for the pH value
2HPO
4-KH
2PO
4Damping fluid, ultimate density (also is phosphoric acid salt Na
2HPO
4-KH
2PO
4Total concn) be 0.02M, the alcoholic acid concentration expressed in percentage by volume is 10%.
Reagent storing solution 2 is for containing the acetonitrile solution of the phenyl-boron dihydroxide ester cpds of resorufin fluorophore shown in the embodiment 1 preparation gained formula I, the ultimate density of this compound is 10 μ M;
3) in sample bottle; The actual sample solution that adds water white transparency or paler colour; Add 2.5mL reagent storing solution 1 and 9 μ L reagent storing solutions 2 more successively; Water and alcohol dilution are to 3mL, and the ultimate density of reagent storing solution 1 and reagent storing solution 2 is respectively 0.02M, 10 μ M, and reagent storing solution 1 is 7.4 alcoholic acid Na for the pH value
2HPO
4-KH
2PO
4Damping fluid, alcoholic acid TV are 10%.After shaking up, leave standstill 15min.Use visual colorimetry, contrast above-mentioned development criteria, get final product the concentration that semiquantitative determination goes out Lucidol in the actual sample.
This step agents useful for same storing solution 1 with 2 with step 2) identical.
Embodiment 4, with the concentration of Lucidol in the Lucidol test kit quantitatively determined flour sample
1) treat the preparation of sample measuring liquid:
Accurately take by weighing known Lucidol addition and be respectively two parts of flour 1g of 73mg/kg, 160mg/kg, place two Glass tubings, add absolute ethyl alcohol 5mL respectively; Supersound extraction 2min; After leaving standstill 1min, get the filtering with microporous membrane of supernatant liquid, filtrate to treating sample measuring liquid with 0.45 μ m.
2) making of typical curve
Take by weighing the 24.2mg Lucidol, be dissolved in the 100mL ethanol, be made into the standard inventory solution of the Lucidol standard substance of 1mM; In sample bottle, add 2.5mL reagent storing solution 1 and 9 μ L reagent storing solutions 2 successively, add the standard inventory solution of the Lucidol of proper volume again; Water and alcohol dilution are respectively the ultimate density of Lucidol to 3mL: 0,0.5,2,4,6,8,10,12,14,16,18,20,22,24,26,30 μ M shake up; Leave standstill 15min, get the 3mL reaction solution in quartz colorimetric utensil, make excitation wavelength with 550nm; Measure the fluorescence intensity at 585nm place; Obtaining series of standards fluorescence intensity of solution F, is reference with the reagent corresponding blank simultaneously, records fluorescence intensity F
0Concentration C (μ g/mL) with Lucidol is an X-coordinate, fluorescence intensity changing value Δ F (Δ F=F-F
0) be ordinate zou, drawing standard curve (like Fig. 2), drawing the corresponding linear regression equation is Δ F=56.2 * C (μ g/mL)+39.1 (R
2=0.997).
Wherein, reagent storing solution 1 is 7.4 alcoholic acid Na for the pH value
2HPO
4-KH
2PO
4Damping fluid, ultimate density (also is phosphoric acid salt Na
2HPO
4-KH
2PO
4Total concn) be 0.02M, the alcoholic acid concentration expressed in percentage by volume is 10%.
Reagent storing solution 2 is for containing the acetonitrile solution of the phenyl-boron dihydroxide ester cpds of resorufin fluorophore shown in the embodiment 1 preparation gained formula I, the ultimate density of this compound is 3 μ M;
3) Lucidol Determination on content in the flour
Treat sample measuring liquid 0.3mL replacement step 2 with the step 1) gained) in used Lucidol standard substance; According to step 2) identical method; Record the fluorescence intensity at 585nm place; Be designated as F ', with F ' substitution step 2) equation of linear regression Δ F=56.2 * C (μ g/mL)+39.1 (R that the gained typical curve is corresponding
2=0.997) in, obtains the concentration of Lucidol in the flour sample to be measured, and then obtain the content of Lucidol in the flour sample to be measured.Repeated experiments three times, experimental result sees the following form 1.
This step agents useful for same storing solution 1 with 2 with step 2) identical.
The detected result of Lucidol content in table 1, the flour sample
Embodiment 5, with the concentration of Lucidol in the Lucidol test kit quantitatively determined antiseptic-germicide sample
1) treat the preparation of sample measuring liquid:
Accurately take by weighing known Lucidol addition and be respectively two parts of gel antiseptic-germicide 1g of 73mg/kg, 160mg/kg; Place two Glass tubings; Add absolute ethyl alcohol 5mL respectively, supersound extraction 2min, leave standstill 1min after; Get the filtering with microporous membrane of supernatant liquid, filtrate to treating sample measuring liquid with 0.45 μ m.
2) making of typical curve
Take by weighing the 24.2mg Lucidol, be dissolved in the 100mL ethanol, be made into the standard inventory solution of the Lucidol standard substance of 1mM; In sample bottle, add 2.5mL reagent storing solution 1 and 9 μ L reagent storing solutions 2 successively, add the Lucidol standard inventory solution of the proper volume that step 1) prepares again; Water and alcohol dilution are respectively the ultimate density of Lucidol to 3mL: 0,0.5,2,4,6,8,10,12,14,16,18,20,22,24,26,30 μ M shake up; Leave standstill 15min, get the 3mL reaction solution in quartz colorimetric utensil, make excitation wavelength with 550nm; Measure the fluorescence intensity at 585nm place; Obtaining series of standards fluorescence intensity of solution F, is reference with the reagent corresponding blank simultaneously, records fluorescence intensity F
0Concentration C (μ g/mL) with Lucidol is an X-coordinate, fluorescence intensity changing value Δ F (Δ F=F-F
0) be ordinate zou, drawing standard curve (like Fig. 2), drawing the corresponding linear regression equation is Δ F=56.2 * C (μ g/mL)+39.1 (R
2=0.997).
Wherein, reagent storing solution 1 is 7.4 alcoholic acid Na for the pH value
2HPO
4-KH
2PO
4Damping fluid, ultimate density (also is phosphoric acid salt Na
2HPO
4-KH
2PO
4Total concn) be 0.02M, the alcoholic acid concentration expressed in percentage by volume is 10%.
Reagent storing solution 2 is for containing the acetonitrile solution of the phenyl-boron dihydroxide ester cpds of resorufin fluorophore shown in the embodiment 1 preparation gained formula I, the ultimate density of this compound is 3 μ M;
3) Lucidol Determination on content in the antiseptic-germicide
Treat sample measuring liquid 0.3mL replacement step 2 with the step 1) gained) in used Lucidol standard substance; According to step 2) identical method; Record the fluorescence intensity at 585nm place; Be designated as F ', with F ' substitution step 2) equation of linear regression Δ F=56.2 * C (μ g/mL)+39.1 (R that the gained typical curve is corresponding
2=0.997) in, obtains the concentration of Lucidol in the antiseptic-germicide sample to be measured, and then obtain the content of Lucidol in the antiseptic-germicide sample to be measured.Repeated experiments three times, experimental result sees the following form 2.
This step agents useful for same storing solution 1 with 2 with step 2) identical.
The detected result of Lucidol content in table 2, the antiseptic-germicide sample
It should be noted last that: it is fluorescent reagent that the foregoing description is only enumerated with the phenyl boronate that contains the resorufin fluorophore (I); Phenyl boronate (I) solution that contains the resorufin fluorophore in the reaction system is respectively 3 μ M, 20mM with the concentration that contains 10% alcoholic acid phosphate buffered saline buffer, the situation of reaction 15min.The result in all the other fluorescent reagents and concentration and reaction times does not list one by one, yet it is not to be used to limit the present invention.Any those skilled in the art under the situation that does not break away from the spirit and scope of the present invention, should make various modifications and change.
Claims (10)
1. phenyl-boron dihydroxide ester cpds shown in the formula I,
Formula I.
2. method for preparing the said compound of claim 1; Comprise the steps: under the condition that catalyzer exists; 7-hydroxyl Phenazoxine ketone sodium salt and 2-bromomethyl benzene boric acid pinacol ester mixing are reacted, and reaction finishes and obtains phenyl-boron dihydroxide ester cpds shown in the said formula I.
3. method according to claim 2 is characterized in that: said catalyzer is selected from least a in organic bases and the mineral alkali;
The molar ratio of said 7-hydroxyl Phenazoxine ketone sodium salt, 2-bromomethyl benzene boric acid pinacol ester and catalyzer is 1: 0.5～5: 0.5～5, is specially 1: 1: 1 or 1: 4: 4 or 1: 1-4: 1-4;
In the said reactions step, temperature is 0 ℃～60 ℃, and preferred 30 ℃, the time is 1～24 hour.
4. method according to claim 3 is characterized in that: said organic bases is selected from least a in triethylamine and the pyridine; Said mineral alkali is selected from least a in salt of wormwood, sodium hydroxide, yellow soda ash and the sodium hydrogencarbonate;
Said being reflected in the organic solvent carried out; Said organic solvent is selected from N, at least a in dinethylformamide, triethylamine, THF and the acetonitrile.
5. a Lucidol detection kit comprises reagent storing solution 1 and reagent storing solution 2; Wherein, said reagent storing solution 1 is a damping fluid; Said reagent storing solution 2 is the solution of the said compound of claim 1.
6. test kit according to claim 5 is characterized in that: in the said reagent storing solution 1, said damping fluid is that the pH value is the phosphate buffered saline buffer of 6～9 organic solvent, and said phosphoric acid salt is selected from Na
2HPO
4, NaH
2PO
4And KH
2PO
4In at least a; Said organic solvent is selected from methyl alcohol, ethanol, DMSO 99.8MIN., THF, acetonitrile and 1, at least a in the 4-dioxane; In the said damping fluid, said phosphatic concentration is 0.01～0.5M, is specially 0.02M; The concentration expressed in percentage by volume of said organic solvent in said damping fluid is 1%～60%, is specially 10%;
In the said reagent storing solution 2, the concentration of the solution of the said compound of said claim 1 is 1-100 μ M, is specially 3-10 μ M; Solvent is selected from acetonitrile, methyl alcohol, ethanol, 1, and at least a in 4-dioxane and the THF is specially acetonitrile.
7. according to the arbitrary described test kit of claim 5-6, it is characterized in that: said Lucidol quick detection kit is made up of with reagent storing solution 2 said reagent storing solution 1.
8. the application of the arbitrary said test kit of claim 5-7 in detecting Lucidol content.
9. application according to claim 8 is characterized in that: the method for said detection Lucidol content comprises the steps:
1) production standard curve:
A) with 550nm as excitation wavelength, the solution of measuring the Lucidol standard substance of a series of different concns is the fluorescence intensity at 585nm place at emission wavelength, is designated as F; And to measure reagent blank be the fluorescence intensity at 585nm place at emission wavelength, is designated as F
0, be X-coordinate with the concentration C of Lucidol, fluorescence intensity changing value Δ F is an ordinate zou, the drawing standard curve;
Wherein, Δ F=F-F
0The solution of the Lucidol standard substance of said a series of different concns is by the standard inventory solution mixing of reagent storing solution 1, reagent storing solution 2 and Lucidol in the arbitrary said Lucidol detection kit of claim 5-8 and water and alcohol dilution and get;
2) content of Lucidol in the detection testing sample:
The said Lucidol standard substance of said step 1) are replaced with said testing sample; Detecting said testing sample according to the said method of said step 1) is the fluorescence intensity at 585nm place at emission wavelength; Be designated as F '; With the said step 1) gained of said F ' substitution typical curve, obtain the concentration of Lucidol in the said testing sample, and then obtain the content of Lucidol in the said testing sample.
10. application according to claim 9; It is characterized in that: in the solution of the Lucidol standard substance of said a series of different concns, the concentration of said Lucidol is followed successively by 0,0.5,2,4,6,8,10,12,14,16,18,20,22,24,26 and 30 μ M;
The volume of the solution of the Lucidol standard substance of said a series of different concns is 3mL;
In the standard inventory solution of said Lucidol, solvent is an ethanol; The concentration of the standard inventory solution of said Lucidol is 1mM;
The reagent storing solution 1 in the arbitrary said Lucidol detection kit of said claim 5-7 and the volume ratio of said reagent storing solution 2 are 2.5mL: 9 μ L;
The corresponding equation of linear regression of the said typical curve of said step 1) is Δ F=56.2 * C+39.1, and wherein, C is the concentration of said Lucidol, and unit is μ g/mL.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107383078A (en) * | 2017-08-03 | 2017-11-24 | 陕西师范大学 | Phenylboric acid ester compounds and the benzoyl peroxide detection kit comprising the compound |
| CN109320537A (en) * | 2018-12-04 | 2019-02-12 | 湖南工业大学 | A kind of soluble two-photon fluorescence probe and its preparation method and application of for flour and in vivo benzoyl peroxide detection |
-
2011
- 2011-12-22 CN CN201110436046.4A patent/CN102532177B/en not_active Expired - Fee Related
Non-Patent Citations (2)
| Title |
|---|
| LO GORTON: "Chemically Modified Electrodes for the Electrocatalytic Oxidation of Nicotinamide Coenzymes", 《JOURNAL OF THE CHEMICAL SOCIETY FARADAY TRANSACTIONS 1》 * |
| WEI CHEN ET AL.: "Simple and fast fluorescence detection of benzoyl peroxide in wheat flour by N-methoxy rhodamine-6G spirolactam based on consecutive chemical reactions", 《ANALYTICA CHIMICA ACTA》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107383078A (en) * | 2017-08-03 | 2017-11-24 | 陕西师范大学 | Phenylboric acid ester compounds and the benzoyl peroxide detection kit comprising the compound |
| CN109320537A (en) * | 2018-12-04 | 2019-02-12 | 湖南工业大学 | A kind of soluble two-photon fluorescence probe and its preparation method and application of for flour and in vivo benzoyl peroxide detection |
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