CN102531134A - Method for using orange peel cooking liquid to eliminate Microcystis aeruginosa in water body - Google Patents
Method for using orange peel cooking liquid to eliminate Microcystis aeruginosa in water body Download PDFInfo
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- CN102531134A CN102531134A CN2011104425365A CN201110442536A CN102531134A CN 102531134 A CN102531134 A CN 102531134A CN 2011104425365 A CN2011104425365 A CN 2011104425365A CN 201110442536 A CN201110442536 A CN 201110442536A CN 102531134 A CN102531134 A CN 102531134A
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Abstract
The invention provides a method for using orange peel cooking liquid to eliminate Microcystis aeruginosa in a water body, which comprises the steps of: firstly cutting orange peels into slices; then putting the sliced orange peels into water, boiling the water and letting the water be boiling for a certain period; and adding the boiled water into the water body to eliminate the Microcystis aeruginosa which is required to be eliminated.
Description
Technical field
The present invention relates to a kind of method of removing Microcystis aeruginosa.
Background technology
Microcystis aeruginosa can produce a kind of natural toxin, is called as Microcystin.Research shows, by tap water and the fishery products that Microcystin pollutes, has brought grave danger can for the health of the mankind and animal.No matter conventional tap water treatment process still with water boil, all is difficult to effectively remove Microcystin.Research shows, even Microcystin still can keep part activity under 300 celsius temperatures.
The vitality of Microcystis aeruginosa is very indomitable, and especially in the water of eutrophication, its speed of growth and reproduction speed are all very fast, and a large amount of Microcystis aeruginosas can make the concentration of the Microcystin in certain waters raise.In the directly dead case of water in the livestock consumption pond that has taken place at present, and confirm that it much is owing to contain the Microcystin of high density in the water and cause that these death incidents have through investigation.
Be directed against in the pollution problem of Microcystis aeruginosa at present, the someone uses gac that Microcystin is adsorbed removal, the chemical reagent of use is also arranged to killing Microcystis aeruginosa.But these methods or cure the symptoms, not the disease, otherwise with high costs, and practicality is not strong.
Summary of the invention
To the problems referred to above, it is low to the invention provides a kind of cost, the method for Microcystis aeruginosa in the removal water body simple to operate.
This method mainly contains following steps and forms:
At first orange peel is cut into small pieces;
Secondly the orange peel of stripping and slicing is put into water, and poach is opened, let its boiling for some time;
The water of boiling is joined in the water body that needs the removal Microcystis aeruginosa.
Through experimental verification, when boiling orange peel, the water ebullient time was advisable with 5-15 minute.
Through experimental verification, the ratio of orange peel and the water of boiling is with 1 (g): 100 (ml)-1 (g): 500 (ml) are advisable.
Through experimental verification, orange peel cooks the ratio of liquid and the water that contains Microcystis aeruginosa with 1: 500-1: 1000 are advisable.
Make in this way growth have significant inhibitory effect, can kill Microcystis aeruginosa well, and use orange peel to cook liquid can not produce secondary pollution, so this method is very environmental protection Microcystis aeruginosa in the water body.And this method can keep for some time the insect killing effect of Microcystis aeruginosa, and cost is very low, operates simple especially.This method is particularly useful for almost immobilized little pond of rural area water body; These little ponds are that the villager provides domestic water on every side on the one hand; To requirements in water quality than higher; The general area in this on the other hand pond is less, and the orange peel that need to use like this cooks the amount of liquid can be not too many, and the villager can use this method that the Microcystis aeruginosa in the water body is administered voluntarily on every side.
Specific embodiment
Through some embodiment the present invention is further explained below, show the invention effect simultaneously.The Microcystis aeruginosa of using among the following embodiment is from the Chaohu in Anhui Province, to obtain also to obtain after the separation and purification, is numbered TH90110.Cultivating the employed substratum of Microcystis aeruginosa is the BG11 liquid nutrient medium, and the pH value of substratum is adjusted to 7.1.Use cell counter (cellometer Auto T4) to carry out cell counting.The ponkan that the orange peel that uses produces as Fujian can be buied on the market.
Embodiment 1
Prepare the 10g orange peel, be cut into fritter, the water of putting into 2000ml heats.By the time reheat stopped heating after water had seethed with excitement after 10 minutes.
Inoculation has the substratum of Microcystis aeruginosa to be loaded in the triangular flask, every bottled 50ml substratum, and inoculation has 1ml to be in the Microcystis aeruginosa suspension of exponential phase of growth in every bottle.
The cooled liquid that cooks is added inoculation and has in the substratum of Microcystis aeruginosa.In every flask culture base, add and cook liquid 0.1ml.These have been added the substratum that cooks liquid and control group all puts into incubator and cultivates.Culture condition is 26 degrees centigrade, and illumination was 8 hours in per 24 hours.Adding is cooked the substratum of liquid and the Microcystis aeruginosa in the substratum in the control group was measured a Microcystis aeruginosa in per 5 days when cultivation begins cell density, measure altogether 5 times.It is multiple that treated and control group all carries out quadruple.
Following table is to measure the result:
| The adding orange peel cooks the substratum (* 10 of liquid 6·ml) | Control group (* 10 6·ml) | |
| The 0th day | 3.32±0.43 | 3.32±0.43 |
| The 5th day | 4.12±0.23 | 15.47±2.34 |
| The 10th day | 1.86±0.26 | 84.56±10.45 |
| The 15th day | 1.41±0.16 | 87.42±9.52 |
| The 20th day | 0.87±0.06 | 57.32±4.11 |
Can see that from the result who measures orange peel cooks liquid extraordinary restraining effect has been played in the growth of Microcystis aeruginosa.
Embodiment 2
Prepare the 10g orange peel, be cut into fritter, the water of putting into 5000ml heats.By the time reheat stopped heating after water had seethed with excitement after 10 minutes.
Inoculation has the substratum of Microcystis aeruginosa to be loaded in the triangular flask, every bottled 50ml substratum, and inoculation has 1ml to be in the Microcystis aeruginosa suspension of exponential phase of growth in every bottle.
The cooled liquid that cooks is added inoculation and has in the substratum of Microcystis aeruginosa.In every flask culture base, add and cook liquid 0.1ml.These have been added the substratum that cooks liquid and control group all puts into incubator and cultivates.Culture condition is 26 degrees centigrade, and illumination was 8 hours in per 24 hours.Adding is cooked the substratum of liquid and the Microcystis aeruginosa in the substratum in the control group was measured a Microcystis aeruginosa in per 5 days when cultivation begins cell density, measure altogether 5 times.It is multiple that treated and control group all carries out quadruple.
Following table is to measure the result:
| The adding orange peel cooks the substratum (* 10 of liquid 6·ml) | Control group (* 10 6·ml) | |
| The 0th day | 3.12±0.21 | 3.12±0.21 |
| The 5th day | 5.63±0.47 | 14.67±1.39 |
| The 10th day | 7.98±0.95 | 90.26±10.06 |
| The 15th day | 5.27±0.46 | 88.35±7.41 |
| The 20th day | 4.71±0.28 | 64.93±5.32 |
Can see that from the result who measures orange peel cooks liquid extraordinary restraining effect has been played in the growth of Microcystis aeruginosa.
Embodiment 3
Prepare the 10g orange peel, be cut into fritter, the water of putting into 1000ml heats.By the time reheat stopped heating after water had seethed with excitement after 15 minutes.
The substratum of Microcystis aeruginosa of growing is loaded in the triangular flask, every bottled 100ml substratum, and the Microcystis aeruginosa in every flask culture base is all near state of saturation.
The cooled liquid that cooks is added inoculation and has in the substratum of Microcystis aeruginosa.In every flask culture base, add and cook liquid 0.15ml.These have been added the substratum that cooks liquid and control group all puts into incubator and cultivates.Culture condition is 26 degrees centigrade, and illumination was 8 hours in per 24 hours.Adding is cooked the substratum of liquid and the Microcystis aeruginosa in the substratum in the control group was measured a Microcystis aeruginosa in per 2 days when cultivation begins cell density, measure altogether 7 times.It is multiple that treated and control group all carries out quadruple.
| The adding orange peel cooks the substratum (* 10 of liquid 6·ml) | Control group (* 10 6·ml) | |
| The 0th day | 91.56±4.66 | 91.56±4.66 |
| The 2nd day | 8.13±1.32 | 90.34±8.21 |
| The 4th day | 6.32±0.89 | 89.02±5.13 |
| The 6th day | 3.10±0.36 | 78.31±6.37 |
| The 8th day | 1.17±0.08 | 72.21±8.31 |
| The 10th day | 1.03±0.06 | 69.34±7.42 |
| The 12nd day | 0.78±0.02 | 66.23±5.56 |
Can find out that from the result who measures orange peel cooks fluid power and substratum, kills Microcystis aeruginosa, the quantity of Microcystis aeruginosa is descended at short notice fast.And this is killed and the effect that suppresses Microcystis aeruginosa can also continue for some time.
Can find out that from top several embodiment orange peel cooks liquid to be had killing really, suppress the effect of its growth Microcystis aeruginosa.Those skilled in the art can make replacement or modification to content of the present invention according to content disclosed by the invention and the art technology of being grasped; But these replacements or modification should not be regarded as breaking away from the present invention's design, and these replacements or modification are all in the interest field that the present invention requires to protect.
Claims (4)
1. method of removing Microcystis aeruginosa in the water body is characterized in that this method is made up of following steps: at first orange peel is cut into small pieces; Secondly the orange peel of stripping and slicing is put into water, and poach is opened, let its boiling for some time; The water of boiling is joined in the water body that needs the removal Microcystis aeruginosa.
2. the method for Microcystis aeruginosa in the removal water body described in the claim 1, when it is characterized in that wherein boiling orange peel, the water ebullient time was advisable with 5-15 minute.
3. the method for Microcystis aeruginosa in the removal water body described in the claim 1 or 2 is characterized in that the orange peel and the ratio of the water of boiling are 1 (g): 100 (ml)-1 (g): 500 (ml).
4. the method for Microcystis aeruginosa in any described removal water body among the claim 1-3 is characterized in that it is 1 with the ratio that contains the water of Microcystis aeruginosa that orange peel cooks liquid: 500-1: 1000.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112299567A (en) * | 2020-09-10 | 2021-02-02 | 苏州贝彩纳米科技有限公司 | Microorganism mixed preparation for inhibiting blue algae and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2011044916A1 (en) * | 2009-10-14 | 2011-04-21 | El Sayed Kamel Morsy Elbaialy | Multipurpose eco-friendly disinfecting composition comprising nano size antibacterial agent |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011044916A1 (en) * | 2009-10-14 | 2011-04-21 | El Sayed Kamel Morsy Elbaialy | Multipurpose eco-friendly disinfecting composition comprising nano size antibacterial agent |
Non-Patent Citations (2)
| Title |
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| 张薛 等: "桔皮水提液对铜绿微囊藻生长的抑制效果研究", 《环境科学研究》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112299567A (en) * | 2020-09-10 | 2021-02-02 | 苏州贝彩纳米科技有限公司 | Microorganism mixed preparation for inhibiting blue algae and preparation method thereof |
| CN112299567B (en) * | 2020-09-10 | 2022-11-25 | 苏州贝彩纳米科技有限公司 | Microorganism mixed preparation for inhibiting blue algae and preparation method thereof |
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Application publication date: 20120704 |