CN102526922A - Microbial preparation for mineralizing and consolidating zinc ions and application method for microbial preparation - Google Patents
Microbial preparation for mineralizing and consolidating zinc ions and application method for microbial preparation Download PDFInfo
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- CN102526922A CN102526922A CN2012100118659A CN201210011865A CN102526922A CN 102526922 A CN102526922 A CN 102526922A CN 2012100118659 A CN2012100118659 A CN 2012100118659A CN 201210011865 A CN201210011865 A CN 201210011865A CN 102526922 A CN102526922 A CN 102526922A
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- urea
- microbial preparation
- zinc ion
- zinc ions
- zinc
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Abstract
The invention discloses a microbial preparation for mineralizing and consolidating zinc ions and an application method for the microbial preparation. The microbial preparation is prepared by compounding 1 liter of bacillus pasteurii liquid and 60 to 80 grams of solid urea, wherein the optical density (OD) of the bacillus pasteurii liquid is 1.2. The microbial preparation is mixed uniformly and sprayed in a metal zinc ion contamination region, wherein the application amount is based on the zinc ion contamination level; urea is added according to the molar ratio of the urea to the zinc ions of 1:1; and the bacillus pasteurii liquid is added based on the mass of the urea. Microorganisms are decomposed under the enzymatic action induced by urea to generate carbonate ions, so that the zinc ions in the solution can be effectively mineralized and consolidated. Environmental impact is low and secondary pollution is avoided.
Description
Technical field
The present invention relates to a kind of microorganism formulation that is used for the fixed zinc ion of mineralising, utilize the enzyme of microorganism under urea is induced to turn usefulness into, decompose the generation carbanion, can be with the Zn in the environment
2+Effectively fixed is the mineral attitude.
Background technology
The pollution of metal, heavy metal has become a global problem; No matter be in the middle of the soil or in the middle of the water body; Various metals, heavy metal have got into the bottom of human foods chain; Serious threat human healthy, has become the important directions that national governments, various countries research institution endeavour to study for the improvement of metal, heavy metal pollution in recent years.
The traditional processing metal and the physico-chemical process of heavy metal are a lot, but exist shortcomings such as investment is big, energy consumption is high, operating difficulties, easy generation secondary pollution to some extent.Along with development of biology, since last century end, people have turned to the microorganism recovery technique with the research emphasis of low content metal and heavy metal pollution improvement gradually.Disposal cost is low, little to ambient influnence, the efficient advantages of higher because of it has for this technology, more and more gets more and more people's extensive concerning.But regrettably, also do not report the method for suitable processing ZINC IN SOIL ion so far.
Summary of the invention
The present invention relates to a kind of microorganism formulation that is used for the fixed zinc ion of mineralising, this microorganism formulation is made up of bi-component, i.e. urea and Pasteur bacillus (Bacillus pasteurii).The use proportioning of microorganism formulation bi-component is: Pasteur bacillus (Bacillus pasteurii) 1L, urea CO (NH
2)
260-80g is sprayed to the heavy metal pollution zone after the mixing.
Technical scheme of the present invention is:A kind of microorganism formulation that is used for the fixed zinc ion of mineralising, by composite the obtaining of solid urea of 1L Pasteur bacillus bacteria liquid and 60 ~ 80g, the OD=1.2 of described Pasteur bacillus bacterium liquid.
Based on the described application process that is used for the microorganism formulation of the fixed zinc ion of mineralising; Be sprayed to metallic zinc ionic soil zone after described microorganism formulation mixed; Amount of application is according to the zinc ion contaminant capacity; Add the urea of corresponding amount according to the ratio of urea and zinc ion mol ratio 1:1, and add the Pasteur bacillus bacterium liquid of corresponding volume according to urea quality.
Beneficial effect:
1. little to ambient influnence, difficult generation secondary pollution;
2. make full use of the nature microorganism resource, not only aboundresources, and technology is simple, and clean environment is with low cost;
3. the mixed solution of microorganism and substrate can penetrate in sand or the soft soil property easily.
Description of drawings
Fig. 1 is the deposit sample X ray diffracting spectrum.
The specific embodiment
Microorganism is to the mineralising mechanism of zinc ion
The main ZnCl that adopts of this experiment
2Experimentize.Pasteur bacillus (Bacillus pasteurii) is meant that it can produce a certain enzyme characteristic in the growth and breeding process, with the Ca in the surrounding environment
2+, Ba
2+, Cu
2+Plasma dissolves one type of next bacterial classification with the form placer deposits of carbonate.Therefore, carbonate mineralized bacterial strain biomineralization zinc ion process can be expressed with following reaction equation:
Zn
2+
?+?CO3
2-
→?ZnCO
3
The obtain manner of described Pasteur's bacillus bacteria liquid is: the Pasteur bacillus is inoculated in culture medium by conventional method, and 170r/min, 37 ℃ of constant temperature take out after cultivating 24h, and the composition of described culture medium such as following table make the OD=1.2 of final bacterium liquid.
Table 1 culture medium is formed
| Nutriment | Quality (g) | Main effect |
| Peptone | 5.0 | Nitrogenous source, carbon source, growth factor are provided |
| Beef extract | 3.0 | Nitrogenous source, carbon source, inorganic salts are provided |
| Deionized water | 1000.0 | As solvent moisture is provided simultaneously |
2 zinc ions are to the influence of carbonate mineralized bacterial strain urease activity
Urea concentration (g/L) changing value with under the Different Zinc ion concentration (mg/L) reflects the influence of zinc ion to the bacterial strain urease activity.
Table 2 Zn
2+Influence (g/L) to the urase capacity of decomposition
| Zn/mg/L | 0 | 3.25mg/L | 10.8mg/L | 21.6mg/L | 108mg/L |
| 0 | 120 | 120 | 120 | 120 | 120 |
| 24h | 70 | 76 | 84 | 86 | 76 |
| 48h | 60 | 65 | 60 | 68 | 60 |
The result shows that in the 48h, the cultured bacterium liquid of 100mL (OD=1.2) can divide the urea of the 6g that terminates an agreement.Zinc ion does not have serious inhibitory action to the urease activity of carbonate mineralized bacterium, can think that zinc ion does not have much influences to the urase capacity of decomposition, and finds when high concentration, to decompose rapid speed, for enzymatic activity facilitation is arranged.
Embodiment 1
1 amount of substrate and component are confirmed
For confirming the capacity of decomposition of bacterial strain, at ambient temperature,, utilize spectrophotometry urea decomposition degree according to the principle of paradime thylaminobenzaldehyde solution and urea colour developing to substrate urea.
According to above result, can know that every 1L bacterium liquid is about 1.1mol-1.2mol to the capacity of decomposition of urea.But in above experiment, urea is the liquid form adding reaction system with 8mol/L, and in practical application, the more convenient practical application of the form of solid, simultaneously, the factor of temperature is considered also.
Add excessive urea (solid) to cultured bacterium liquid 100mL, place 30 ° of C, 15 ° of C environment respectively, observe urea concentration and change.
Table 3 environment temperature influences the urase capacity of decomposition
The result finds, at 30 ℃--and in 15 ℃ the environment, urea concentration is reduced to 39g/L to 60g/L by 120g/L, and bacterium liquid (OD=1.2) the urea decomposition amount of 10mL is between 6g-8g.
Therefore, can confirm the decomposition amount that urea is final, and obtain the component ratio of bacterium liquid and urea in the preparation: 1L bacterium liquid (OD=1.2)+60-80g urea (solid) through above experiment
2 practical implementations
Liquor zinci chloridi with 100mL1mol/L is a system to be repaired, to wherein adding the cultured bacterium liquid of 100mL (OD=1.2), substrate urea 8g.Behind the 48h, filtering precipitate, oven dry obtains filtrate, and product is carried out XRD analysis, confirms that the composition main component is Zn
5(CO
3)
2(OH)
6
Claims (2)
1. microorganism formulation that is used for the fixed zinc ion of mineralising is characterized in that: by composite the obtaining of solid urea of 1L Pasteur bacillus bacteria liquid and 60 ~ 80g, and the OD=1.2 of described Pasteur bacillus bacterium liquid.
2. based on the described application process that is used for the microorganism formulation of the fixed zinc ion of mineralising of claim 1; It is characterized in that; Be sprayed to metallic zinc ionic soil zone after described microorganism formulation mixed; Amount of application is according to the zinc ion contaminant capacity, adds the urea of corresponding amount according to the ratio of urea and zinc ion mol ratio 1:1, and adds the Pasteur bacillus bacterium liquid of corresponding volume according to urea quality.
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|---|---|---|---|
| CN2012100118659A CN102526922A (en) | 2012-01-16 | 2012-01-16 | Microbial preparation for mineralizing and consolidating zinc ions and application method for microbial preparation |
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|---|---|---|---|
| CN2012100118659A CN102526922A (en) | 2012-01-16 | 2012-01-16 | Microbial preparation for mineralizing and consolidating zinc ions and application method for microbial preparation |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103266592A (en) * | 2013-05-27 | 2013-08-28 | 东南大学 | Method utilizing phosphate mineralized bacteria to solidify and loosen loose sand particles |
| CN106269850A (en) * | 2016-09-27 | 2017-01-04 | 西南科技大学 | A kind of method utilizing carbonate mineralized bacterium thiobacillus denitrificans Combined Treatment lead ion to pollute |
| CN108457242A (en) * | 2018-01-25 | 2018-08-28 | 重庆大学 | A kind of microorganism reef method of coastal waters chiltern bank slope |
| JP2018527181A (en) * | 2015-08-27 | 2018-09-20 | バイオセメント テクノロジーズ， インコーポレイテッド | Bioremediation of geological materials contaminated with heavy metals by resident microorganisms |
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| JPH1113306A (en) * | 1997-06-25 | 1999-01-19 | Fujita Corp | Building supporting method at installation of vibration isolation device |
| CN1923720A (en) * | 2006-09-13 | 2007-03-07 | 东南大学 | Method of consolidating heavy metal ion by microorganism |
| CN101644047A (en) * | 2009-07-23 | 2010-02-10 | 东南大学 | Method of adhering loose sands employing microbial mineralization |
| CN102139278A (en) * | 2010-12-14 | 2011-08-03 | 东南大学 | Microbial preparation for mineralized and consolidated copper ions and use method thereof |
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2012
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH01113306A (en) * | 1987-10-26 | 1989-05-02 | Takeo Tabata | Controlling drug against pomacea canaliculata lamarck and method for controlling |
| JPH1113306A (en) * | 1997-06-25 | 1999-01-19 | Fujita Corp | Building supporting method at installation of vibration isolation device |
| CN1923720A (en) * | 2006-09-13 | 2007-03-07 | 东南大学 | Method of consolidating heavy metal ion by microorganism |
| CN101644047A (en) * | 2009-07-23 | 2010-02-10 | 东南大学 | Method of adhering loose sands employing microbial mineralization |
| CN102139278A (en) * | 2010-12-14 | 2011-08-03 | 东南大学 | Microbial preparation for mineralized and consolidated copper ions and use method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103266592A (en) * | 2013-05-27 | 2013-08-28 | 东南大学 | Method utilizing phosphate mineralized bacteria to solidify and loosen loose sand particles |
| CN103266592B (en) * | 2013-05-27 | 2015-08-19 | 东南大学 | A kind of method utilizing phosphate mineralized bacterium consolidation loosen loose sand particle |
| JP2018527181A (en) * | 2015-08-27 | 2018-09-20 | バイオセメント テクノロジーズ， インコーポレイテッド | Bioremediation of geological materials contaminated with heavy metals by resident microorganisms |
| CN106269850A (en) * | 2016-09-27 | 2017-01-04 | 西南科技大学 | A kind of method utilizing carbonate mineralized bacterium thiobacillus denitrificans Combined Treatment lead ion to pollute |
| CN108457242A (en) * | 2018-01-25 | 2018-08-28 | 重庆大学 | A kind of microorganism reef method of coastal waters chiltern bank slope |
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Application publication date: 20120704 |