CN102516335A - Method for extracting flavonoid compound from marigold residue - Google Patents
Method for extracting flavonoid compound from marigold residue Download PDFInfo
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- CN102516335A CN102516335A CN2011104062997A CN201110406299A CN102516335A CN 102516335 A CN102516335 A CN 102516335A CN 2011104062997 A CN2011104062997 A CN 2011104062997A CN 201110406299 A CN201110406299 A CN 201110406299A CN 102516335 A CN102516335 A CN 102516335A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Abstract
The invention discloses a method for extracting a flavonoid compound from a marigold residue. The method comprises the steps of: (1) conducting extraction on the marigold residue with an acetone water solution so as to obtain a crude extraction solution; (2) concentrating the crude extraction solution so as to obtain a concentrated solution, which is then cooled to obtain a crude extract; and (3) eluting the crude extract with n-hexane so as to obtain a fat-soluble impurity removed crude extract, which is then eluted by a sodium hydrogen phosphate-citric acid buffer solution, thus obtaining a product. The method of the invention takes a marigold residue as the raw material, which is subjected to crushing, sieving, as well as rapid and effective extraction of a flavonoid substance by a water solution of the organic solvent. And through concentration, precipitation, drying, and purification, an obtained yellow substance has a flavonoid compound content of over 90g RE/100g. The method provided in the invention has the advantages of reasonable technical parameters, simple technological path, simple and safe operation, high yield, low equipment and production cost, and great practical value as well as social and economic benefits.
Description
Technical field
The present invention relates to a kind of method of from the Flower of Aztec Marigold residue, extracting flavonoid compound.
Background technology
Flower of Aztec Marigold is one of draft potted flower of mainly cultivating of China, and is widely used in indoor and outdoor surroundings and arranges.Along with the nutrient health industry development, more and more to the demand of Flower of Aztec Marigold in the world.Flower of Aztec Marigold not only has outside the ornamental value, and also has good pharmaceutical use, and its staple that plays pharmacological action is compositions such as Flavonoid substances, carotenoids, terpene and essential oil.Flower of Aztec Marigold with and congener in contain common flavones such as Quercetin, kaempferol, ampelopsin, more flavones is that the form with glycosides exists, like patulitrin, quercetagitrin, kaempferol glycosides.Flavonoid substances has the anti-oxidant activity of removing radical, stronger antitumor action, and antitumous effect, the effect of reducing blood-fat, hypoglycemic, prevention cardio-cerebrovascular diseases is widely used in industries such as food, makeup, healthcare products and medicine.The Flower of Aztec Marigold residue is the by product of Flower of Aztec Marigold processing oleo-resinous; It contains especially Flavonoid substances of abundant active function composition; The xenthophylls in extracting Flower of Aztec Marigold, annual all have a large amount of Flower of Aztec Marigold residues to be abandoned as industrial waste, or use as feed.This affects to environment, but also causes the very big wasting of resources.Therefore, the Flavonoid substances in the Flower of Aztec Marigold residue is extracted purifying, help the comprehensive utilization of Flower of Aztec Marigold resource.
Summary of the invention
The purpose of this invention is to provide a kind of method of from the Flower of Aztec Marigold residue, extracting flavonoid compound.
A kind of method of from the Flower of Aztec Marigold residue, extracting flavonoid compound provided by the invention comprises the steps:
(1) with aqueous acetone solution to said Flower of Aztec Marigold residue extract crude extract;
(2) with said crude extract concentrate liquid concentrator; Said liquid concentrator through cool off crude extract;
(3) said crude extract obtains removing the crude extract of lipid-soluble substance through the normal hexane wash-out; The said crude extract that removes lipid-soluble substance promptly gets product through Sodium phosphate, dibasic-citric acid solution wash-out.
In the above-mentioned method, said Flower of Aztec Marigold residue can be the residue behind supercritical carbon dioxide extraction; The granularity of said Flower of Aztec Marigold residue can be 20 orders～80 orders, is specially 20 orders, 30 orders, 40 orders or 60 orders; The pH value of said Sodium phosphate, dibasic-citric acid solution can be 2.2～6, specifically can be 2.2,4 or 5.
In the above-mentioned method, described in the step (1) in the aqueous acetone solution quality percentage composition of acetone can be 20%～95%, specifically can be 40%, 50%, 60% or 70%; The proportioning of said Flower of Aztec Marigold residue and said aqueous acetone solution can be: the said Flower of Aztec Marigold residue of every 1g needs the said aqueous acetone solution of (9～35) ml, needs 10ml, 20ml or the said aqueous acetone solution of 30ml like the said Flower of Aztec Marigold residue of every 1g.
In the above-mentioned method; Extract described in the step (1) and can in the water bath with thermostatic control shaking table, carry out, the temperature of said extraction can be 20 ℃～70 ℃, specifically can be 40 ℃, 50 ℃ or 60 ℃; The time of said extraction can be 20min～120min, specifically can be 40min, 60min, 80min or 120min.
In the above-mentioned method, extracting solution described in the step (2) can be (4～8) with the volume parts ratio of said liquid concentrator: 1, specifically can be 5: 1; Said refrigerative temperature can be 0 ℃～20 ℃, and as 4 ℃, the said refrigerative time can be 10min～180min, like 120min; The said method in said cooling back also can comprise centrifugal treating, lyophilize or vacuum drying step.
In the above-mentioned method, in the step (3), the proportioning of said crude extract and normal hexane can be: the said crude extract of every 1g needs (9～35) ml normal hexane, needs 9ml, 12ml or the said normal hexane of 15ml like the said crude extract of every 1g; The said crude extract that removes lipid-soluble substance can be with the proportioning of Sodium phosphate, dibasic-citric acid solution: the said crude extract that removes lipid-soluble substance of every 1g needs the said Sodium phosphate, dibasic-citric acid solution of (9～35) ml, need 9ml, 12ml or the said Sodium phosphate, dibasic-citric acid solution of 15ml like the said crude extract that removes lipid-soluble substance of every 1g.
In the above-mentioned method, in the step (3), said normal hexane wash-out and Sodium phosphate, dibasic-citric acid solution wash-out all can carry out in the water bath with thermostatic control shaking table; The inferior number average of said normal hexane wash-out and Sodium phosphate, dibasic-citric acid solution wash-out can be 1～5, specifically can be 1,3 or 5.
In the above-mentioned method, in the step (3), the temperature of said normal hexane wash-out can be 20 ℃～60 ℃, specifically can be 40 ℃, 50 ℃ or 60 ℃, and the time of said wash-out can be 20min～70min, specifically can be 30min, 40min or 50min.
In the above-mentioned method; In the step (3), the temperature of said Sodium phosphate, dibasic-citric acid solution wash-out can be 30 ℃～90 ℃, specifically can be 40 ℃, 50 ℃ or 60 ℃; The time of said wash-out can be 20min～90min, specifically can be 30min, 40min or 50min.
The flavonoid compound that method provided by the invention is extracted can be rutin.
The present invention is a raw material with the Flower of Aztec Marigold residue, and raw material pulverizing is sieved, and fast, effectively extracts Flavonoid substances through aqueous solutions of organic solvent, obtains yellow shape material more than the flavonoid content 90g RE/100g through concentrated, deposition, drying, purification; The processing parameter of method provided by the invention is reasonable, and operational path is simple, safety simple to operate, and yield is high, and equipment and production cost are low, have bigger practical value and economic results in society; Therefore, of the present invention is that the method that raw material extracts purifying flavonoids compounds has very strong practicality characteristics, suitable applying with the Flower of Aztec Marigold residue.
Embodiment
Employed experimental technique is ordinary method like no specified otherwise among the following embodiment.
Used material, reagent etc. like no specified otherwise, all can obtain from commercial sources among the following embodiment.
Used Flower of Aztec Marigold residue is through the residue behind the supercritical carbon dioxide extraction among the following embodiment.
The concrete grammar that content of flavonoids is measured among the following embodiment is: the sample 0.5mL that gets proper concn; Add in the 15mL scale test tube, be supplemented to 4mL with deionized water, the sodium nitrite in aqueous solution that adds 0.3mL 5% (w/v) shakes up; Adding 3mL 1% (w/v) aluminum chloride aqueous solution behind the 5min shakes up; Add the aqueous sodium hydroxide solution of the 1mol/L of 2mL behind the 6min, add deionized water again to 10mL, the 510nm place measures light absorption value behind the 10min; Blank replaces sample to react with deionized water; Content of flavonoids is represented with rutin equivalent (Rutin Equivalent, RE/g butt).
The preparation of standardized solution: accurately take by weighing rutin mark article 0.075g, in the volumetric flask as for 25mL, it is settled to scale with methyl alcohol.Pipette 5mL, 4mL, 3mL, 2mL and 1mL respectively in the 10mL volumetric flask with transfer pipet then; With methanol constant volume to scale; Obtain concentration and be respectively 1.5,1.2,0.9,0.6 and the solution of 0.3mg/mL; Measure according to the method described above, maximum absorption is arranged, obtain the typical curve between the concentration of absorbance and rutin mark article at 510nm place at the 510nm place.
The extract that embodiment 1～4 obtains is measured according to the method described above, can be obtained content of flavonoids in the extract according to the typical curve that obtains.
The extraction of embodiment 1, flavonoid compound
(1) is ready to the Flower of Aztec Marigold residuum feedstocks, and it was pulverized 20 mesh sieves;
(2) be that quality percentage composition that 1: 10 (g/ml) is added to acetone is in 40% the aqueous acetone solution with the Flower of Aztec Marigold residue of 100g mixing particle diameter according to mass volume ratio; (100r/min) extracts 40min in 40 ℃ of following water bath with thermostatic control shaking tables, and suction filtration obtains the crude extract of Flavonoid substances;
(3) crude extract is concentrated 5 times and get liquid concentrator; With liquid concentrator in 4 ℃ leave standstill the cooling 2h, remove supernatant, drying obtains crude extract 6.27g;
(4) repeating above-mentioned steps and produce the 30g crude extract, is that 1: 9 (g/ml) is added in the normal hexane according to mass volume ratio, and (100r/min) 30min wash-out is 1 time in 40 ℃ of following water bath with thermostatic control shaking tables, the crude extract 29.04g of the lipid-soluble substance that is removed;
(5) be that 1: 9 (g/ml) to be added to the pH value be in Sodium phosphate, dibasic-citric acid solution of 2.2 with the crude extract of the weeding of grease soluble substance that obtains according to mass volume ratio; (100r/min) 30min wash-out is 1 time in 40 ℃ of following water bath with thermostatic control shaking tables; Dry making with extra care obtains 24.06g, and content is the flavonoid compound of 84.15g RE/100g.
The extraction of embodiment 2, flavonoid compound
(1) is ready to the Flower of Aztec Marigold residuum feedstocks, and it was pulverized 40 mesh sieves;
(2) be that quality percentage composition that 1: 20 (g/ml) is added to acetone is in 60% the aqueous acetone solution with the Flower of Aztec Marigold residue of 100g mixing particle diameter according to mass volume ratio; (100r/min) extracts 80min in 50 ℃ of following water bath with thermostatic control shaking tables, and suction filtration obtains the crude extract of Flavonoid substances;
(3) crude extract is concentrated 5 times and get liquid concentrator; With liquid concentrator in 4 ℃ leave standstill the cooling 2h, remove supernatant, drying obtains crude extract 7.28g;
(4) repeating above-mentioned steps and produce the 30g crude extract, is that 1: 12 (g/ml) is added in the normal hexane according to mass volume ratio, and (100r/min) 40min wash-out is 3 times in 50 ℃ of following water bath with thermostatic control shaking tables, the crude extract 28.52g of the lipid-soluble substance that is removed;
(5) be that 1: 12 (g/ml) to be added to the pH value be in Sodium phosphate, dibasic-citric acid solution of 4 with the crude extract of the weeding of grease soluble substance that obtains according to mass volume ratio; (100r/min) 40min wash-out is 3 times in 50 ℃ of following water bath with thermostatic control shaking tables; Dry making with extra care obtains 21.07g, and content is the flavonoid compound of 91.54g RE/100g.
The extraction of embodiment 3, flavonoid compound
(1) is ready to the Flower of Aztec Marigold residuum feedstocks, and it was pulverized 60 mesh sieves;
(2) be that quality percentage composition that 1: 30 (g/ml) is added to acetone is in 70% the aqueous acetone solution with the Flower of Aztec Marigold residue of 100g mixing particle diameter according to mass volume ratio; (100r/min) extracts 120min in 60 ℃ of following water bath with thermostatic control shaking tables, and suction filtration obtains the crude extract of Flavonoid substances;
(3) crude extract is concentrated 5 times and get liquid concentrator; With liquid concentrator in 4 ℃ leave standstill the cooling 2h, remove supernatant, drying obtains crude extract 7.85g;
(4) repeating above-mentioned steps and produce the 30g crude extract, is that 1: 15 (g/ml) is added in the normal hexane according to mass volume ratio, and (100r/min) 50min wash-out is 5 times in 60 ℃ of following water bath with thermostatic control shaking tables, the crude extract 28.23g of the lipid-soluble substance that is removed;
(5) be that 1: 15 (g/ml) to be added to the pH value be in Sodium phosphate, dibasic-citric acid solution of 5 with the crude extract of the weeding of grease soluble substance that obtains according to mass volume ratio; (100r/min) 50min wash-out is 5 times in 60 ℃ of following water bath with thermostatic control shaking tables; Dry making with extra care obtains 20.24g, and content is the flavonoid compound of 90.63g RE/100g.
The extraction of embodiment 4, flavonoid compound
(1) is ready to the Flower of Aztec Marigold residuum feedstocks, and it was pulverized 30 mesh sieves;
(2) be that quality percentage composition that 1: 10 (g/ml) is added to acetone is in 50% the aqueous acetone solution with the Flower of Aztec Marigold residue of 100g mixing particle diameter according to mass volume ratio; (100r/min) extracts 60min in 40 ℃ of following water bath with thermostatic control shaking tables, and suction filtration obtains the crude extract of Flavonoid substances;
(3) crude extract is concentrated 5 times and get liquid concentrator; With liquid concentrator in 4 ℃ leave standstill the cooling 2h, remove supernatant, drying obtains crude extract 6.52g;
(4) repeating above-mentioned steps and produce the 30g crude extract, is that 1: 9 (g/ml) is added in the normal hexane according to mass volume ratio, and (100r/min) 30min wash-out is 5 times in 40 ℃ of following water bath with thermostatic control shaking tables, the crude extract 28.66g of the lipid-soluble substance that is removed;
(5) be that 1: 9 (g/ml) to be added to the pH value be in Sodium phosphate, dibasic-citric acid solution of 5 with the crude extract of the weeding of grease soluble substance that obtains according to mass volume ratio; (100r/min) 30min wash-out is 5 times in 40 ℃ of following water bath with thermostatic control shaking tables; Dry making with extra care obtains 21.36g, and content is the flavonoid compound of 90.42g RE/100g.
Claims (9)
1. a method of from the Flower of Aztec Marigold residue, extracting flavonoid compound comprises the steps:
(1) with aqueous acetone solution to said Flower of Aztec Marigold residue extract crude extract;
(2) with said crude extract concentrate liquid concentrator; Said liquid concentrator through cool off crude extract;
(3) said crude extract obtains removing the crude extract of oil-soluble impurities through the normal hexane wash-out; The said crude extract that removes oil-soluble impurities promptly gets product through Sodium phosphate, dibasic-citric acid solution wash-out.
2. method according to claim 1 is characterized in that: said Flower of Aztec Marigold residue is the residue behind supercritical carbon dioxide extraction; The granularity of said Flower of Aztec Marigold residue is 20 orders～80 orders; The pH value of said Sodium phosphate, dibasic-citric acid solution is 2.2～6.
3. method according to claim 1 and 2 is characterized in that: described in the step (1) in the aqueous acetone solution quality percentage composition of acetone be 20%～95%; The proportioning of said Flower of Aztec Marigold residue and said aqueous acetone solution is: the said Flower of Aztec Marigold residue of every 1g needs the said aqueous acetone solution of (9～35) ml.
4. according to arbitrary described method among the claim 1-3, it is characterized in that: be extracted in the water bath with thermostatic control shaking table described in the step (1) and carry out, the temperature of said extraction is 20 ℃～70 ℃, and the time of said extraction is 20min～120min.
5. according to arbitrary described method among the claim 1-4, it is characterized in that: crude extract described in the step (2) is (4～8) with the volume parts ratio of said liquid concentrator: 1; Said refrigerative temperature is 0 ℃～20 ℃, and the said refrigerative time is 10min～180min; The said method in said cooling back also comprises centrifugal treating, lyophilize or vacuum drying step.
6. according to arbitrary described method among the claim 1-5, it is characterized in that: in the step (3), the proportioning of said crude extract and normal hexane is: the said crude extract of every 1g needs (9～35) ml normal hexane; The said crude extract that removes oil-soluble impurities with the proportioning of Sodium phosphate, dibasic-citric acid solution is: the said said Sodium phosphate, dibasic-citric acid solution of crude extract need (9～35) ml that removes oil-soluble impurities of every 1g.
7. according to arbitrary described method among the claim 1-6, it is characterized in that: in the step (3), said normal hexane wash-out and Sodium phosphate, dibasic-citric acid solution wash-out all carries out in the water bath with thermostatic control shaking table; The number of times of said normal hexane wash-out and Sodium phosphate, dibasic-citric acid solution wash-out is 1～5.
8. according to arbitrary described method among the claim 1-7, it is characterized in that: in the step (3), the temperature of said normal hexane wash-out is 20 ℃～60 ℃, and the time of said wash-out is 20min～70min.
9. according to arbitrary described method among the claim 1-8, it is characterized in that: in the step (3), the temperature of said Sodium phosphate, dibasic-citric acid solution wash-out is 30 ℃～90 ℃, and the time of said wash-out is 20min～90min.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105541775A (en) * | 2016-02-26 | 2016-05-04 | 中国农业大学 | Industrial preparation method of quercetagetin |
| CN105693675A (en) * | 2014-11-28 | 2016-06-22 | 天津耀宇生物技术有限公司 | A method of extracting flavonoid compounds from tagetes erecta |
| WO2020259565A1 (en) * | 2019-06-28 | 2020-12-30 | 晨光生物科技集团股份有限公司 | Industrializable method for rapidly and efficiently extracting xanthophyll and quercetagetin |
| CN112999252A (en) * | 2021-03-03 | 2021-06-22 | 晨光生物科技集团股份有限公司 | Industrial method for continuously extracting high-content marigold flavone |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102190646A (en) * | 2011-03-29 | 2011-09-21 | 中国农业大学 | Method for preparing high-purity quercetagetin |
-
2011
- 2011-12-08 CN CN2011104062997A patent/CN102516335A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102190646A (en) * | 2011-03-29 | 2011-09-21 | 中国农业大学 | Method for preparing high-purity quercetagetin |
Non-Patent Citations (2)
| Title |
|---|
| 宋大巍 等: "超临界萃取万寿菊黄色素工艺参数的优化", 《农产品加工 学刊》 * |
| 张宇 等: "万寿菊茎叶化学成分研究", 《中药材》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105693675A (en) * | 2014-11-28 | 2016-06-22 | 天津耀宇生物技术有限公司 | A method of extracting flavonoid compounds from tagetes erecta |
| CN105693675B (en) * | 2014-11-28 | 2018-07-27 | 天津耀宇生物技术有限公司 | A method of extracting flavone compound component from marigold |
| CN105541775A (en) * | 2016-02-26 | 2016-05-04 | 中国农业大学 | Industrial preparation method of quercetagetin |
| WO2020259565A1 (en) * | 2019-06-28 | 2020-12-30 | 晨光生物科技集团股份有限公司 | Industrializable method for rapidly and efficiently extracting xanthophyll and quercetagetin |
| CN112999252A (en) * | 2021-03-03 | 2021-06-22 | 晨光生物科技集团股份有限公司 | Industrial method for continuously extracting high-content marigold flavone |
| CN112999252B (en) * | 2021-03-03 | 2022-05-20 | 晨光生物科技集团股份有限公司 | Industrial method for continuously extracting high-content marigold flavone |
| WO2022183908A1 (en) * | 2021-03-03 | 2022-09-09 | 晨光生物科技集团股份有限公司 | Industrial method for continuously extracting high-content marigold flavonoid |
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Application publication date: 20120627 |