CN102512483A - Medicinal composition soft capsules for improving anoxia tolerance and preparation method for medicinal composition soft capsules - Google Patents
Medicinal composition soft capsules for improving anoxia tolerance and preparation method for medicinal composition soft capsules Download PDFInfo
- Publication number
- CN102512483A CN102512483A CN2012100048904A CN201210004890A CN102512483A CN 102512483 A CN102512483 A CN 102512483A CN 2012100048904 A CN2012100048904 A CN 2012100048904A CN 201210004890 A CN201210004890 A CN 201210004890A CN 102512483 A CN102512483 A CN 102512483A
- Authority
- CN
- China
- Prior art keywords
- preparation
- medicinal composition
- extraction
- composition soft
- soft capsule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides medicinal composition soft capsules for improving anoxia tolerance and a preparation method for the medicinal composition soft capsules, and belongs to the technical field of health-care products and the fields of medicines, food and the health-care products. The preparation method comprises the following steps of: putting crushed integripetal rhodiola herb rhizomes and bee glue into a supercritical kettle, and extracting at the temperature of between 20 and 80 DEG C under the pressure of between 20 and 80 MPa for 1 to 6 hours to obtain a composition extract; dissolving 3 grams of composition extract subjected to supercritical extraction in edible oil in an amount which is 0.5 to 1.5 times that of the composition extract in a constant volume mode, adding a preservative and an antioxidant, and mixing uniformly; and loading the uniformly-mixed medicines into a soft capsule machine to obtain the soft capsules. According to the preparation method, an extraction process is simple, the quality is controllable, the content of active ingredients of the extract is high, and the cost is low, so the preparation method is suitable for industrial production; and the medicinal composition soft capsules are convenient to take, and have an effect of improving sleep.
Description
Technical field
The present invention relates to health product technology field, specifically relate to improve soft capsule of processing with Radix Rhodiolae active substance and bee-glue composition of anoxia endurance and preparation method thereof.
Background technology
In recent years; Country strengthens the construction to Tibet; Many batches of in order to develop the plateau resource; The personnel that aid Tibet in defendance and the plateau that builds up one's motherland, borderland and by get into Tibet to the Chinese people bodyguard on plateau interiorly interiorly, they need long-term work and live under the environment of high altitude anoxia, and altitude sickness is worked normally and is lived to influence their the puzzlement that they bring.The Tibet train is open-minded in addition, has opened the convenient door of Tibet tourism, and people are that the original landscape and the natural views that see Tibet swarm forward; But altitude sickness makes most people painful worried; The headache that altitude sickness is brought, unable tired out, make people lose the excitement of playing; Must not only in the hotel, have a rest, even the somebody does not appreciate, and the plateau is honourable has just gone back at once.Some are not hung back to going the highland tour once mentioning altitude sickness to the people on plateau.
The area of height above sea level more than 3000 meters is called the highlands.The highlands atmospheric pressure is low, is merely the half the of Plain, and oxygen content is merely 60% of Plain in the air, thus formed with low pressure, anoxia, low temperature, solar radiation is strong, the temperature difference is big, the plateau climate of strong wind, dry equipotential distinguishing feature.The people of low altitude area; After getting into the plateau; Because the rapid variation of aspect such as weather has surpassed the self-regulating limit of normal person's body, in height above sea level location more than 4000 meters; Have the people of 60%-100% that acute anoxia reaction or disease can take place approximately, rise symptom common headache, giddy arranged, have palpitation, breathe hard, inappetence, nausea and vomiting, abdominal distention, uncomfortable in chest, chest pain, fatigue and weak, facial slight edema, cheilosis etc.When serious blood pressure increase, palpitating speed, even comatose state appears.The somebody can occur then that unusual exaltation, polylogia are multi-lingual, instability of gait, hallucination, insomnia etc.
Be used to treat the medicine instant effect of altitude sickness at present, but side reaction is comparatively serious.Be usually used in preventing and treating the edema disease that causes because of the plateau discomfort like acetazolamide, furosemide.And use the acetazolamide common adverse reactions that sleepy, face and numb limbs and tense tendons sense are arranged, can cause kidney complications etc.Too much use untoward reaction such as slight feeling sick possibly appear in furosemide, diarrhoea, drug eruption, pruritus, blurred vision.And inhibitor commonly used, inosine sheet, glucose etc. often receive individual technical ability condition influence, and dose is bigger, and effect is not too obvious.So Development and Production improves anoxia endurance efficiently and goods that side effect is little are too impatient to wait.
The Radix Rhodiolae that 2010 editions pharmacopeia are recorded is dry root and the rhizome of Crassulaceae plant Radix Rhodiolae Rhodiola crenulata (Hook.f.et Thoms.) H.Ohba.Autumn, scape was excavated after withered, removed rough bark, cleaned, and dried.
Radix Rhodiolae is China's tradition medicinal plants, generally is grown in the many stones and the coarse sand mountain region of height above sea level 3500-5100 rice.Because Radix Rhodiolae has adapted to extremely abominable changeable natural environment such as anoxia, high and cold, dry, strong ultraviolet radiation, diurnal temperature great disparity, so possessed that anti-hypoxia, anti-blast, the anti-width of cloth are penetrated, the special drug effect of anti-cold drying.Radix Rhodiolae contains trace element and 7 kinds of vitamin in unique Radix Rhodiolae aglycon butyl alcohol and 18 seed amino acids (wherein 8 kinds is that human body is necessary), 34, has nourishing and medicinal efficacy.Wherein the peculiar rhodioside of Radix Rhodiolae (Salidroside) has anti-high altitude anoxia, resisting fatigue, radioprotective, defying age, enhancing human body immunity power, effects such as two-ways regulation body body function.The medical expert proves that through zoopery rhodioside shows that Radix Rhodiolae has the effect that strengthens serum LPH vigor in the research for the mouse anti-reflecting fatigue effect; Accelerated decomposition lactic acid, the blood lactic acid concn descends, and reduces OUR; Increase the keeping effect, obviously prolong heavy burden mice swimming time, exercise tolerance obtains significant prolongation and continues; Strengthen the deposit of hepatic glycogen, muscle glycogen, have the obvious anti-fatigue effect, this medicine of zoopery results suggest can improve the aerobic metabolism process in the important organ tissue such as heart and brain; The level of atrial natriuretic peptide in significantly improving animal liver, myocardium content of glycogen and improving in the animal cardiopulmonary tissue; Thereby alleviate the animal viscera infringement that causes because of anoxia, and then can effectively improve the life span of animal under hypoxia condition, alleviate the altitude sickness that causes because of anoxia, intensive ultraviolet radiation etc.
Current main method for distilling to the rhodosin in the Radix Rhodiolae, rhodioside isoreactivity composition is water extraction and alcohol extracting method, and the water law extraction cost is low, and method is simple; Alcohol method extraction cost is high than the former; But the content of soluble solid (yield) is higher with respect to water extraction in the extracting solution, so conventional red Herba hylotelephii erythrosticti extracts active ingredients is with alcohol extraction (extraction of surplus Hua Hezhi gift rhodiola active ingredient and flexibility (adaptability) of operation research food industry science and technology .2002.23 (8) .-47-49) more commonly used. but traditional extraction process exist extraction time long, technology is complicated, efficient is low, industrial pollution is big and to defectives such as target compound extraction non-selectivities.
The propolis that 2010 editions pharmacopeia are recorded is the dry secretions of Apidae insecticide apis mellifera Linnaeus Apis mellifera L..More than from beehive, collecting summer, remove impurity.
Propolis is the resin of Apis from plant spore and trunk collection, sneaks into a kind of gluey solid content of viscosity with fragranced of formation such as Apis mandibular gland secretions and Cera Flava.Propolis contains 55% resin of having an appointment, 30% Cera Flava, 10% ethereal oil and pollen of 5% etc., and its complex chemical composition contains phenol, acid, alcohol, ester, phenol, ether, terpene, alkene, sterol and various trace elements.Propolis has the effect that tonify deficiency is weak, change turbid fat, also can be used for the empty senilism of body, hyperlipemia.The big quantity research done of Chinese scholars shows in recent years; Propolis has important physical effects such as treatment cardiovascular, diabetes, dermatosis, gastrointestinal disease, anticancer, enhance immunity, anti-inflammation; Become the focus of health food research field, also more and more deep to the component separating of performance efficacy effect in the propolis with analysis.
Supercritical extraction (Supercritica fluid extraction; SFE) technology is to utilize selected supercritical fluid; All be higher than under its critical temperature and the critical pressure condition at temperature, pressure, this fluid density is near liquid condition, and diffusion coefficient and viscosity are near gaseous state; Not only have the extracting substances ability suitable, and have the mass transfer effect suitable with gaseous solvent with liquid flux.In addition, through in supercritical fluid, adding the entrainer of variety classes and quantity, can improve selectivity and efficient that supercritical fluid extracts target compound greatly.
Fructus Perillae be first regulation of Ministry of Public Health be medicine be again one of 60 kinds of article of food.Contain abundant fat and protein in the Fructus Perillae, can be used for producing edible oil and protein.Recently. the polyunsaturated fatty acid that is rich in the Fructus Perillae oil causes extensive attention both domestic and external.Proof after deliberation, Fructus Perillae oil has blood pressure lowering, and blood fat reducing is anticancer and suppress hematoblastic gathering, reduces the formation of thrombosis. vision protection. improve the effect of intelligence.
Existing patent report Radix Rhodiolae has the effect that improves anoxia endurance.A kind of extraction Radix Rhodiolae method is disclosed in the Chinese patent (CN200510068041.5): add 4-10 times of water boil, filtered in 1-4 hour, collect filtrating concentrating; The ethanol that adds 70%-100%; Leave standstill, filter, concentrate; Get Radix Rhodiolae extract and propolis and processed a kind of compositions that can improve anoxia endurance.Chinese patent (CN200710017501.0) discloses a kind of food composition and method for preparing of altitude sickness prevention; Wherein also mention rhodioside and have the effect that improves anoxia endurance; Its method for distilling is to use the 60%-65% reflow of alcohol to extract temperature to be 40-55 ℃, reclaim under reduced pressure ethanol.So Radix Rhodiolae is clear efficacy aspect the raising anoxia endurance.But all be to use Different concentrations of alcohol to extract in above-mentioned two pieces of patents.
In addition, used in the Chinese patent (CN200510129948.8) with ethanol and be that entrainer uses CO
2-supercritical extraction method extracts the tyrosol in the Radix Rhodiolae: the Radix Rhodiolae dry root, pulverize, and sieving is material.Pack in the extraction kettle, extraction temperature is 40 ℃, CO
2Fluid pressure 400Par, during entrainer ethanol flow velocity 0.3ml/min, extraction time 2h.The extraction ratio of tyrosol is 1.637%, than improving 6.54 times through traditional circumfluence method (0.25%) extraction ratio.In addition, use microbial enzyme liquid that the Radix Rhodiolae rhizome is carried out enzymolysis processing in addition after, re-use ethanol and carry out reflux, extract,, and then extract the Radix Rhodiolae extraction ratio.
Be to use ethanol extraction and water extraction respectively in the Chinese patent (CN200510068041.5), with both extract mixing adding adjuvants with propolis and Radix Rhodiolae.Wherein the proportioning of propolis and Radix Rhodiolae is 2.5: 3.
In sum, the extraction process of existing patent report is all complicated, and cost is high, is unsuitable for application.Up to now, do not see as yet both at home and abroad any about adopting CO
2-supercritical process directly extracts document and the patent report that improves the effective ingredient of anoxia endurance in Radix Rhodiolae and the propolis.
Summary of the invention
The present invention provides a kind of composition soft that is used to improve anoxia endurance and preparation method thereof, and is complicated to solve the prior art extraction process, and cost is high, is unsuitable for the problem of application.This patent is with after propolis and the Radix Rhodiolae mixing, adopts CO
2-supercritical process directly extracts, and with reference to 2010 editions officinal doses and preliminary experiment result, the ratio of confirming propolis and Radix Rhodiolae is 1: 10-20.Compare with above-mentioned patent on method for distilling and both ratios bigger difference is arranged.This product adopts CO
2-supercritical extraction technique extracts rhodioside and chrysin in the propolis and the galangin in the long white Radix Rhodiolae; Its content of effective is significantly increased than traditional extraction process; Guarantee and improved the quality of natural goods product, constant product quality, no hazardous solvent residual.
The technical scheme that the present invention takes is:
(1) get Radix Rhodiolae rhizome and propolis after the pulverizing, put into the supercritical still, 20 ℃-80 ℃ of extraction temperature, extracting pressure is 20MPa-80MPa, extraction time is 1h-6h, the compositions extract.
(2) supercritical extraction compositions extract is dissolved in the food oil, 3g compositions extract is settled in the 0.5-1.5 edible oil doubly, add antiseptic, antioxidant mix homogeneously again;
(3) encapsulating machine on the medicine of above-mentioned mix homogeneously is made composition soft; Be about to process adhesive tape after gelatin, the G & W dissolving, and the medicine of above-mentioned mix homogeneously is placed between two blocks of offset plates, form with the punching block compacting; Through after the assay was approved, get composition soft.
Extraction described in the present invention is a kind of in Fructus Perillae oil, olive oil, soybean oil, Oleum sesami, Semen Maydis oil, Oleum Sesami, Oleum Gossypii semen, Oleum Ricini, Oleum Camelliae, safflower oil, the middle long-chain fatty acid ester with food oil; Two kinds; Or two or more mixture, wherein preferentially select Fructus Perillae oil, olive oil.
Antiseptic described in the present invention is selected from methyl hydroxybenzoate, ethyl hydroxybenzoate, benzoic acid, the sorbic acid one or both or two or more mixture.
The consumption of the antiseptic described in the present invention is: methyl hydroxybenzoate 0.015-0.2% (W/V), ethyl hydroxybenzoate 0.015-0.2% (W/V), benzoic acid 0.01-0.1% (W/V), sorbic acid 0.05-0.2% (W/V).
Antioxidant described in the present invention is selected from V
C, V
E, one or both or two or more mixture in benzoic acid, citric acid and salt thereof, sorbitol, sodium sulfite, sodium sulfite, sodium pyrosulfite, the sodium thiosulfate.
The consumption of the antioxidant described in the present invention is: V
C0.01-0.1% (W/V), V
E0.001-0.05% (V/V).
Soft capsule shell described in the present invention, formula proportion is: gelatin: glycerol: water=1: 0.2-0.6: 0.5-1.5.
This product adopts CO
2-supercritical extraction method has the extraction efficiency height, and solvent load is little, fast and accurately characteristics.Compare with traditional method and to have following beneficial effect:
(1) CO
2-supercritical extraction is environmentally friendly technology.Supercritical extraction lacks than the step of dissolution extraction relatively, and flow process is short, and operating parameter also is easy to control.
(2) because CO
2-supercritical extraction ability depends on fluid density, thereby is easy to make carbon dioxide and extract be divided into biphase (gas, liquid) rapidly through regulating pressure and temperature, reaches promptly, optionally extracts the purpose of effective ingredient in the Chinese medicine.Not only extraction efficiency is high, power consumption is low, and extract impurity reduces, effective ingredient is highly enriched.
(3) supercritical extraction solvent for use CO
2Have nontoxic, tasteless, do not fire not the burn into low price, be easy to make with extra care, be easy to advantages such as recovery.Compare no solvent residue with the conventional solvent extraction method.Not only avoid simultaneously the use of a large amount of organic solvents, prevented that also leaching process is to the harm of health with to the pollution of environment.
(4) supercritical extraction CO
2-supercritical extraction also has the antioxidation sterilization functions, helps guaranteeing and improving the quality of natural goods product.
The specific embodiment
Below through specific embodiment, specific embodiments of the invention remakes further detailed description:
Embodiment:
(1) supercritical extraction: get Radix Rhodiolae rhizome and propolis after the pulverizing, put into the supercritical still, 20 ℃-80 ℃ of extraction temperature, extracting pressure is 20MPa-80MPa, extraction time is 1h-6h, the compositions extract.
(2) the compositions extract that supercritical extraction is obtained is dissolved in and extracts with in the food oil, and 3g compositions extract is settled in the 0.5-1.5 edible oil doubly, adds antiseptic, antioxidant mix homogeneously again;
(3) encapsulating machine on the medicine of mix homogeneously in (two) is made the Radix Rhodiolae soft capsule; Be about to process adhesive tape after gelatin, the G & W dissolving, and the medicine of mix homogeneously in (two) is placed between two blocks of offset plates, last encapsulating machine is suppressed with punching block; Through after the assay was approved, get composition soft.
Extraction described in the present invention is a kind of in Fructus Perillae oil, olive oil, soybean oil, Oleum sesami, Semen Maydis oil, Oleum Sesami, Oleum Gossypii semen, Oleum Ricini, Oleum Camelliae, safflower oil, the middle long-chain fatty acid ester with food oil; Or two kinds; Or two or more mixture; Wherein preferred Fructus Perillae oil, olive oil, best with Fructus Perillae oil especially.
The assay of rhodioside in the Radix Rhodiolae
With octadecylsilane key and silica gel is filler, is mobile phase with methanol-water=15: 85; The detection wavelength is 275nm.It is an amount of to get the rhodioside reference substance respectively, and accurate the title decides, and adds methanol and processes the solution that every 1ml contains 0.5mg, as the reference substance solution of rhodioside.Get the supercritical extraction Extraction oil, precision weighing 10ml is in separatory funnel, and with acetonitrile extraction 2 times, each 10ml merges supernatant, and evaporate to dryness is used the 5ml dissolve with methanol, filters, and gets subsequent filtrate, as need testing solution.Accurate respectively reference substance solution and each 10ul of need testing solution of drawing injects high performance liquid chromatograph, carries out assay according to HPLC (2010 editions appendix VID of Chinese Pharmacopoeia), promptly gets.
The assay of chrysin and galangin in the propolis
With octadecylsilane key and silica gel is filler, is mobile phase with methanol-0.15% phosphoric acid solution (64: 36); The detection wavelength is 268nm.Get the chrysin reference substance, the galangin reference substance is an amount of, accurate claims surely, add methanol and process the solution that every 1ml contains chrysin 30ug, galangin 20ug respectively, as the reference substance solution of propolis.Get the supercritical extraction Extraction oil, precision weighing 10ml is in separatory funnel, and with acetonitrile extraction 2 times, each 10ml merges supernatant, and evaporate to dryness is used the 5ml dissolve with methanol, filters, and gets subsequent filtrate, as need testing solution.Accurate respectively reference substance solution and each 10ul of need testing solution of drawing injects high performance liquid chromatograph, carries out assay according to HPLC (2010 editions appendix VID of Chinese Pharmacopoeia), promptly gets.
Pharmacodynamic experiment
One, mice normal pressure anoxia enduring experiment
40 mices are divided into 4 groups at random, 10 every group.The normal saline group is through mouse tail vein injection normal saline 10ml/kg; Positive drug control group is through mouse peritoneal injection propranolol hydrochloride 20mg/kg; Experimental group gives present composition 1g/kg, 2mg/kg through the tail vein respectively.20min after the administration places mice respectively in the 250ml ground wide mouthed bottle that is placed with sodica calx to seal, and observes the mice time-to-live (every bottle of degeneration silica gel 10g, wide mouthed bottle are put 1 mice for every bottle with the preceding correction capacity that all is filled with water, and are index with the respiratory arrest).
Experimental result shows; Compare with the normal saline group; The high low dose group of the present composition, propranolol hydrochloride group be the ability significant prolongation mice time-to-live (P＜0.05) all, shows that the present composition and propranolol hydrochloride all have the effect that improves the mice hypoxia-bearing capability; Object height low dosage of the present invention effect is weaker than propranolol hydrochloride slightly, but does not have significant difference (P＜0.05).
Two, mice specificity myocardial ischemia experiment
40 mices are divided into 4 groups at random, 10 every group.Normal saline group mice and isoproterenol group mice are all through tail vein injection saline 10ml/kg; The positive drug control group mice is through tail vein injection propranolol hydrochloride 20mg/kg; Experimental mice gives present composition 1g/kg, 2g/kg through the tail vein respectively.40min after the administration, except that the normal saline group, all the other respectively organize the equal subcutaneous injection isoproterenol of mice 15ml/kg, behind the 10min, mice is put into the 250ml ground wide mouthed bottle that degeneration silica gel is housed respectively seal, the record mice time-to-live.
Experimental result shows that the isoproterenol group mice time-to-live obviously shortens (P＜0.05) than the normal saline group, shows that isoproterenol can increase the mice oxygen consumption, shortens the anoxia in mice following time-to-live of state.Compare with the normal saline group; Object height low dose group of the present invention, propranolol hydrochloride group be the ability significant prolongation mice time-to-live (P＜0.05) all; Show that the present composition and propranolol hydrochloride all can resist the myocardial oxygen consumption increase that isoproterenol causes, have tangible oxygen lack resistant function; And object height dosage of the present invention effect is suitable with propranolol hydrochloride slightly.
Three, mice low pressure anoxia enduring experiment
Get 40 of mices, body weight 18-22g, the male and female dual-purpose is divided into 5 groups at random.Be respectively the normal saline matched group, the abdominal cavity gives the isometric(al) normal saline; Object height of the present invention, in, low dose group, lumbar injection 0.5,1 respectively, 2mg/kg; The positive drug control group mice is through lumbar injection propranolol hydrochloride 20mg/kg.Behind the gastric infusion 10min, mice is placed respectively in the vacuum drying oven, is decompressed to 21.328kpa with vacuum pump, and keep constant relatively, the record mouse diing time.
The result shows that the present composition can obviously prolong the time-to-live of mice under the reduced pressure, has obvious dose-effect relationship, with normal saline matched group comparing difference remarkable (P＜0.05, P＜0.01, P＜0.001).Dosage pharmacological action and propranolol hydrochloride are suitable in the present composition, and effect of high dosage is better than propranolol hydrochloride slightly.
Claims (9)
1. one kind is used to improve anoxia endurance medicinal composition soft capsule and preparation method thereof, and method for preparing is characterized in that:
(1) get Radix Rhodiolae rhizome and propolis after the pulverizing, put into the supercritical still, 20 ℃-80 ℃ of extraction temperature, extracting pressure is 20MPa-80MPa, extraction time is 1h-6h, the compositions extract;
(2) the compositions extract that supercritical extraction is obtained is dissolved in and extracts with in the food oil, and 3g compositions extract is settled in the 0.5-1.5 edible oil doubly, adds antiseptic, antioxidant mix homogeneously again;
(3) gelatin, G & W are processed adhesive tape after dissolving, and the medicine of mix homogeneously in (two) is placed between two blocks of offset plates, last encapsulating machine is suppressed with punching block, through after the assay was approved, gets composition soft.
2. a kind of medicinal composition soft capsule that is used to improve anoxia endurance according to claim 1 and preparation method thereof, it is characterized in that: the ratio of propolis and Radix Rhodiolae is 1: 10-20.
3. a kind of anoxia endurance medicinal composition soft capsule and preparation method thereof that is used to improve according to claim 2 is characterized in that: extract with the preferred Fructus Perillae oil of food oil, olive oil.
4. a kind of anoxia endurance medicinal composition soft capsule and preparation method thereof that is used to improve according to claim 3 is characterized in that: extracting and using food oil is best with the Fructus Perillae oil.
5. a kind of anoxia endurance medicinal composition soft capsule and preparation method thereof that is used to improve according to claim 1; It is characterized in that: antiseptic is selected from methyl hydroxybenzoate, ethyl hydroxybenzoate, benzoic acid, the sorbic acid a kind of; Or two kinds, or two or more mixture; The consumption of antiseptic is: methyl hydroxybenzoate 0.015-0.2% (W/V), ethyl hydroxybenzoate 0.015-0.2% (W/V), benzoic acid 0.01-0.1% (W/V), sorbic acid 0.05-0.2% (W/V).
6. a kind of anoxia endurance medicinal composition soft capsule and preparation method thereof that is used to improve according to claim 1, it is characterized in that: antioxidant is selected from V
C, V
E, a kind of in benzoic acid, citric acid and salt thereof, sorbitol, sodium sulfite, sodium sulfite, sodium pyrosulfite, the sodium thiosulfate, or two kinds, or two or more mixture; The consumption of antioxidant is: V
C0.01-0.1% (W/V), V
E0.001-0.05% (V/V).
7. a kind of anoxia endurance medicinal composition soft capsule and preparation method thereof that is used to improve according to claim 1, it is characterized in that: the formula proportion of soft capsule shell is: gelatin: glycerol: water=1: 0.2-0.6: 0.5-1.5.
8. one kind is used to improve anoxia endurance medicinal composition soft capsule and preparation method thereof, and the assay of rhodioside in the Radix Rhodiolae is characterized in that: with octadecylsilane key and silica gel is filler, is mobile phase with methanol-water=15: 85; The detection wavelength is 275nm.It is an amount of to get the rhodioside reference substance respectively, and accurate the title decides, and adds methanol and processes the solution that every 1ml contains 0.5mg, as the reference substance solution of rhodioside.Get the supercritical extraction Extraction oil, precision weighing 10ml is in separatory funnel, and with acetonitrile extraction 2 times, each 10ml merges supernatant, and evaporate to dryness is used the 5ml dissolve with methanol, filters, and gets subsequent filtrate, as need testing solution.Accurate respectively reference substance solution and each 10ul of need testing solution of drawing injects high performance liquid chromatograph, carries out assay according to 2010 editions one appendix VID HPLC of Chinese Pharmacopoeia.
9. one kind is used to improve anoxia endurance medicinal composition soft capsule and preparation method thereof; The assay of chrysin and galangin in the propolis; It is characterized in that: with octadecylsilane key and silica gel is filler, is mobile phase with methanol-0.15% phosphoric acid solution (64: 36); The detection wavelength is 268nm; Get the chrysin reference substance, the galangin reference substance is an amount of, accurate claims surely, add methanol and process the solution that every 1ml contains chrysin 30ug, galangin 20ug respectively, as the reference substance solution of propolis; Get the supercritical extraction Extraction oil, precision weighing 10ml is in separatory funnel, and with acetonitrile extraction 2 times, each 10ml merges supernatant, and evaporate to dryness is used the 5ml dissolve with methanol, filters, and gets subsequent filtrate, as need testing solution; Accurate respectively reference substance solution and each 10ul of need testing solution of drawing injects high performance liquid chromatograph, carries out assay according to 2010 editions one appendix VID HPLC of Chinese Pharmacopoeia.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100048904A CN102512483A (en) | 2012-01-10 | 2012-01-10 | Medicinal composition soft capsules for improving anoxia tolerance and preparation method for medicinal composition soft capsules |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100048904A CN102512483A (en) | 2012-01-10 | 2012-01-10 | Medicinal composition soft capsules for improving anoxia tolerance and preparation method for medicinal composition soft capsules |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102512483A true CN102512483A (en) | 2012-06-27 |
Family
ID=46283736
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012100048904A Pending CN102512483A (en) | 2012-01-10 | 2012-01-10 | Medicinal composition soft capsules for improving anoxia tolerance and preparation method for medicinal composition soft capsules |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102512483A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109303786A (en) * | 2018-11-27 | 2019-02-05 | 山东省药学科学院 | A kind of composition of resist oxygen lack and its application |
| CN109608428A (en) * | 2018-12-29 | 2019-04-12 | 杭州师范大学 | Micro- extracting method of phenolic compound in a kind of propolis |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1861109A (en) * | 2005-05-09 | 2006-11-15 | 孙毅 | Compound medicine for improving tolerance of oxygen deficit, and its prepn. method |
| CN1927875A (en) * | 2005-09-09 | 2007-03-14 | 浙江大学宁波理工学院 | CO2 supercritical extraction method of rhodiola root glycoside with ethanol as entrainer |
| CN101204403A (en) * | 2007-12-13 | 2008-06-25 | 吴江市方霞企业信息咨询有限公司 | Supercritocal extracting and processing propolis |
-
2012
- 2012-01-10 CN CN2012100048904A patent/CN102512483A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1861109A (en) * | 2005-05-09 | 2006-11-15 | 孙毅 | Compound medicine for improving tolerance of oxygen deficit, and its prepn. method |
| CN1927875A (en) * | 2005-09-09 | 2007-03-14 | 浙江大学宁波理工学院 | CO2 supercritical extraction method of rhodiola root glycoside with ethanol as entrainer |
| CN101204403A (en) * | 2007-12-13 | 2008-06-25 | 吴江市方霞企业信息咨询有限公司 | Supercritocal extracting and processing propolis |
Non-Patent Citations (1)
| Title |
|---|
| 李杏花等: "超临界萃取技术在中药成分提取分离中的应用研究进展", 《亚太传统医药》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109303786A (en) * | 2018-11-27 | 2019-02-05 | 山东省药学科学院 | A kind of composition of resist oxygen lack and its application |
| CN109608428A (en) * | 2018-12-29 | 2019-04-12 | 杭州师范大学 | Micro- extracting method of phenolic compound in a kind of propolis |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102895432A (en) | Prescription and preparation method of rejoicing powder having new dosage form | |
| CN104740412B (en) | A kind of Medicament for Alcoholism composition and preparation method thereof | |
| CN102430046B (en) | Aquatic product alive-keeping agent and preparation method and application thereof | |
| CN102283895A (en) | Preparation technology and production method of integrated novel Lingguizhugan decoction dosage form | |
| CN103735675B (en) | Medicinal composition with liver protecting function and preparation method of medicinal composition | |
| CN103211957B (en) | Alcohol relieving and liver protecting composition and application thereof | |
| CN102512483A (en) | Medicinal composition soft capsules for improving anoxia tolerance and preparation method for medicinal composition soft capsules | |
| CN101301330A (en) | Codonopsis pilosula pollen capsules and preparation thereof | |
| CN102949681B (en) | Composition for preventing or treating colds, and its preparation method | |
| CN102302545A (en) | Dai medicine extract with blood-sugar reducing activity, preparation method, composition and application | |
| TWI612968B (en) | A use of an extract of asplenium australasicum (j. sm.) hook. | |
| CN102283984A (en) | Preparation technology of integrated novel Gualou Xiebai Baijiu decoction dosage form | |
| CN102462740A (en) | Pharmaceutical composition for relieving alcoholism and protecting liver and preparation method thereof | |
| CN102293883B (en) | Chinese medicinal composition for treating child acute upper respiratory tract infection, and preparation method and application thereof | |
| CN100453073C (en) | Compound radical lobelia dripping pill and its preparing method | |
| CN101721444B (en) | Saussurea involucrate self-micro-emulsion, preparation method thereof and application thereof | |
| CN104382139B (en) | A kind of beverage for acute alcoholism and preparation method thereof | |
| CN102847092A (en) | Preparation process of integration-type novel formulation of Evodia rutaecarpa decoction and production method thereof | |
| CN1079396A (en) | Intelligence-enhancing pharmaceutical preparation | |
| CN102526647A (en) | Preparation technology and production method of novel dual-purpose decoction integrated dosage form | |
| CN102120000A (en) | Composition of effective parts of traditional Chinese medicine preparation for resisting Coxsackie virus B and preparation method thereof | |
| CN102861278A (en) | Lipid-lowering extract from effective parts of sharpleaf galangal fruit and preparation and application thereof | |
| CN102284024A (en) | Preparation technology of integrated novel Weijing decoction dosage form | |
| CN103028095A (en) | Preparation technology and production method of integrated new dosage form of dry ginger and Radix aconiti lateralis preparata decoction | |
| CN100348178C (en) | 'Ganjing' dripping pills for treating liver disease and its preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20120627 |