CN102512440A - Application of calcium gluconate for drug preparation - Google Patents
Application of calcium gluconate for drug preparation Download PDFInfo
- Publication number
- CN102512440A CN102512440A CN2011104200677A CN201110420067A CN102512440A CN 102512440 A CN102512440 A CN 102512440A CN 2011104200677 A CN2011104200677 A CN 2011104200677A CN 201110420067 A CN201110420067 A CN 201110420067A CN 102512440 A CN102512440 A CN 102512440A
- Authority
- CN
- China
- Prior art keywords
- calcium gluconate
- polyinosini
- poly
- application
- purposes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicinal Preparation (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention relates to an application of calcium gluconate for drug preparation. When poly I: C is prepared, a small amount of calcium gluconate is added, the compactibility of a poly I: C double-chain structure can be increased, accordingly, the stability of the poly I: C is increased, and nuclease is resisted to degraded the poly I: C.
Description
Technical field:
The present invention relates to the purposes of the purposes, particularly calcium gluconate of a kind of calcium gluconate in the preparation medicine in preparation polyinosini preparation.
Background technology:
Polyinosini is the copolymer of polyinosinic acid and poly, is a kind of efficient interferon inducer, has broad-spectrum antiviral, stimulates functions such as engulfing, adjust immunity of organism, antitumor, anti-some antibacterial and protozoan infection.1958 by U.S. scientist David R.Davies with Alexander Rich finds first and synthetic.1967, people such as U.S. scientist Field and Tytell found that polyinosini has powerful efficient interferon-induced function when the efficient interferon inducers of research.From then on, worldwide started one polyinosini research boom.824 groups of the Chinese Academy of Sciences in 1969 begin to develop polyinosini in China; Heredity institute of the Chinese Academy of Sciences in 1975 and Tianjin Gourmet Powder Factory and Institute of Micro-biology of the Chinese Academy of Sciences join together to have formed the polyinosini cooperative groups in succession and have found out suitable industrial combined process; And creatively used new prescription, and applied to clinical gradually.
Summary of the invention:
The present invention relates to the purposes of a kind of calcium gluconate in the preparation medicine.
The purposes of a kind of calcium gluconate in the preparation medicine when it is characterized in that preparing polyinosini, adds calcium gluconate, can strengthen the compactness of polyinosini duplex structure, thereby increase the stability of polyinosini, can resist the degraded of nuclease to polyinosini.
The purposes of a kind of calcium gluconate in the preparation medicine is characterized in that the pharmaceutical formulation of polyinosini is: polyinosinic acid, poly, calcium gluconate.
The present invention has adopted new pharmaceutical formulation; Be combined at polyinosinic acid, poly and add a small amount of calcium gluconate on the basis of polyinosini; Advantage is to strengthen the compactness of polyinosini duplex structure; Thereby increased the stability of polyinosini, can resist the degraded of nuclease, made it give full play to its drug effect polyinosini.
The specific embodiment:
Through specific embodiment of the present invention given below, can further be well understood to content of the present invention, but they not to qualification of the present invention.
Instance 1
The purposes of a kind of calcium gluconate in the preparation medicine comprises the steps:
1. get the about 900ml of water for injection, stirring and dissolving sodium hydrogen phosphate 1.55g, sodium dihydrogen phosphate 0.26g, sodium chloride 8.77g.
2. this buffer is divided equally into 2 parts, and is heated to 50 ℃.A dissolving polyinosinic acid 0.49g, another part dissolving poly 0.51g.
3. after fully dissolving, under slowly stirring, poly solution is sneaked in the polyinosinic acid solution mixing.
4. in solution, slowly dripping calcium gluconate solution to final concentration is 4mmol, and under slowly stirring, keeps 30 minutes.
5. be cooled to room temperature, be settled to 1000ml.
6.0.45 μ m filter element filtering, embedding.
Instance 2
The purposes of a kind of calcium gluconate in the preparation medicine comprises the steps:
1. get the about 900ml of water for injection, stirring and dissolving sodium hydrogen phosphate 1.55g, sodium dihydrogen phosphate 0.26g, sodium chloride 8.77g.
2. this buffer is divided equally into 2 parts, and is heated to 50 ℃.A dissolving polyinosinic acid 0.98g, another part dissolving poly 1.02g.
3. after fully dissolving, under slowly stirring, poly solution is sneaked in the polyinosinic acid solution mixing.
4. in solution, slowly dripping calcium gluconate solution to final concentration is 4mmol, and under slowly stirring, keeps 30 minutes.
5. be cooled to room temperature, be settled to 1000ml.
6.0.45 μ m filter element filtering, embedding.
Instance 3
Inhibition of proliferation effect (external) to the adenocarcinoma of stomach cell strain
According to 10
4The density of cells/well moves into cell in the hole of 96 orifice plates, and the polyinosini that added instance 2 on the 2nd day therein continues to cultivate.Measure the survivaling cell number respectively at 72h and 96h with mtt assay, its result is as shown in the table.
The anticancer experimental result of cell shows that the anticancer effect of polyinosini obviously strengthens behind the adding calcium gluconate.
Claims (2)
1. the calcium gluconate purposes in the preparation medicine; It is characterized in that: when the preparation polyinosini, add calcium gluconate, can strengthen the compactness of polyinosini duplex structure; Thereby increased the stability of polyinosini, can resist the degraded of nuclease polyinosini.
2. the calcium gluconate purposes in the preparation medicine, according to claim 1, it is characterized in that: the pharmaceutical formulation of polyinosini is: polyinosinic acid, poly, calcium gluconate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104200677A CN102512440A (en) | 2011-12-15 | 2011-12-15 | Application of calcium gluconate for drug preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104200677A CN102512440A (en) | 2011-12-15 | 2011-12-15 | Application of calcium gluconate for drug preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102512440A true CN102512440A (en) | 2012-06-27 |
Family
ID=46283694
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011104200677A Pending CN102512440A (en) | 2011-12-15 | 2011-12-15 | Application of calcium gluconate for drug preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102512440A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103599071A (en) * | 2013-11-08 | 2014-02-26 | 杭州美亚药业有限公司 | Preparation method of polyinosinic-polycytidylic acid dry powder |
-
2011
- 2011-12-15 CN CN2011104200677A patent/CN102512440A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103599071A (en) * | 2013-11-08 | 2014-02-26 | 杭州美亚药业有限公司 | Preparation method of polyinosinic-polycytidylic acid dry powder |
| CN103599071B (en) * | 2013-11-08 | 2016-01-27 | 杭州美亚药业股份有限公司 | A kind of preparation method of polyinosinic-polycytacidic acidic dry powder |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| NZ628328A (en) | Rna formulation for immunotherapy | |
| CN101851276B (en) | Preparation method and activity detection for antibacterial peptides | |
| CN102643361A (en) | Nano selenium micromolecular microbial polysaccharide as well as preparation method and application thereof | |
| CN204563298U (en) | Electric-heat medicine brick health care bed | |
| CN104073900B (en) | Application of ionic liquid to polyurethane complex fiber | |
| CN102512440A (en) | Application of calcium gluconate for drug preparation | |
| CN102617718B (en) | Bran coat antitumor active protein, as well as preparation method and applications thereof | |
| CN104288170A (en) | Application of echinacoside in anti-tumor medicaments | |
| CN104073901A (en) | Application of nitrogen-contained ionic liquid to polyurethane complex fiber | |
| CN104587507A (en) | Mixed deodorant and preparation method thereof | |
| CN103254260B (en) | The method of symmetric double nucleoside diphosphate disodium salt is synthesized by nucleosides hydrogen monophosphite | |
| CN104095847A (en) | Pharmaceutical composition containing imipenem cilastatin sodium and preparation thereof | |
| CN102633899A (en) | Selenized microbial exopolysaccharide, and preparation method and uses thereof | |
| CN103638030A (en) | Application of nitrosylruthenium complexes taking 2-chloro-8-hydroxyquinoline as ligand | |
| CN105641337A (en) | Compound Yushang ointment | |
| CN102228469A (en) | Nano-selenium particles and preparation method thereof | |
| CN1219519C (en) | Exterior use prepn. for treating skin trauma | |
| CN103396456B (en) | A kind of Divalent double-core platinum complex and preparation method thereof | |
| CN103462951B (en) | Application of scopariusins in preparation of medicine treating or preventing canker sore | |
| CN102659849B (en) | Platinum (II) complex with antitumor activity and preparation method thereof | |
| CN202456428U (en) | Body-building socks for children | |
| CN105456272A (en) | Application of Biscarpamontamine A in preparing medicine for increasing red blood cells | |
| CN101723982A (en) | Platinum complex compound with antitumor activity and synthesis method thereof | |
| CN103638010B (en) | Application of Artoxanthochromane in antibacterial drugs | |
| CN103667406A (en) | Preparation method of inulin source recombinant allophycocyanin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C05 | Deemed withdrawal (patent law before 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120627 |