CN102499260A - Application of copper nanomaterial with copper/carbon-core/shell structure in antibiosis - Google Patents
Application of copper nanomaterial with copper/carbon-core/shell structure in antibiosis Download PDFInfo
- Publication number
- CN102499260A CN102499260A CN2011103084772A CN201110308477A CN102499260A CN 102499260 A CN102499260 A CN 102499260A CN 2011103084772 A CN2011103084772 A CN 2011103084772A CN 201110308477 A CN201110308477 A CN 201110308477A CN 102499260 A CN102499260 A CN 102499260A
- Authority
- CN
- China
- Prior art keywords
- copper
- core
- carbon
- shell structure
- nano
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 title claims abstract description 122
- 239000002086 nanomaterial Substances 0.000 title claims abstract description 29
- 239000010949 copper Substances 0.000 title abstract description 87
- 229910052802 copper Inorganic materials 0.000 title abstract description 85
- 230000003115 biocidal effect Effects 0.000 title abstract description 21
- 241000233866 Fungi Species 0.000 claims abstract description 26
- 230000001954 sterilising effect Effects 0.000 claims abstract description 24
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 20
- 241000894006 Bacteria Species 0.000 claims abstract description 14
- 241001465178 Bipolaris Species 0.000 claims description 22
- 230000000844 anti-bacterial effect Effects 0.000 claims description 20
- 241000223259 Trichoderma Species 0.000 claims description 10
- 241000222122 Candida albicans Species 0.000 claims description 8
- 229940095731 candida albicans Drugs 0.000 claims description 8
- 241000191967 Staphylococcus aureus Species 0.000 claims description 7
- 241000588724 Escherichia coli Species 0.000 claims description 5
- 241000295644 Staphylococcaceae Species 0.000 claims description 3
- 241000532447 Chlorociboria aeruginosa Species 0.000 claims description 2
- 241000371644 Curvularia ravenelii Species 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 14
- 239000003814 drug Substances 0.000 abstract description 10
- 230000008901 benefit Effects 0.000 abstract description 5
- 239000003242 anti bacterial agent Substances 0.000 abstract description 4
- 230000007246 mechanism Effects 0.000 abstract description 2
- 206010059866 Drug resistance Diseases 0.000 abstract 1
- 239000002105 nanoparticle Substances 0.000 description 59
- 239000002245 particle Substances 0.000 description 25
- 150000001875 compounds Chemical class 0.000 description 16
- 238000012360 testing method Methods 0.000 description 15
- 230000012010 growth Effects 0.000 description 14
- 238000002474 experimental method Methods 0.000 description 13
- 239000000523 sample Substances 0.000 description 12
- 239000000243 solution Substances 0.000 description 12
- 206010070834 Sensitisation Diseases 0.000 description 9
- 229940079593 drug Drugs 0.000 description 9
- 230000008313 sensitization Effects 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 7
- 239000002131 composite material Substances 0.000 description 7
- 229910001431 copper ion Inorganic materials 0.000 description 7
- BERDEBHAJNAUOM-UHFFFAOYSA-N copper(I) oxide Inorganic materials [Cu]O[Cu] BERDEBHAJNAUOM-UHFFFAOYSA-N 0.000 description 7
- 229940112669 cuprous oxide Drugs 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 239000011258 core-shell material Substances 0.000 description 5
- KRFJLUBVMFXRPN-UHFFFAOYSA-N cuprous oxide Chemical compound [O-2].[Cu+].[Cu+] KRFJLUBVMFXRPN-UHFFFAOYSA-N 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 206010015150 Erythema Diseases 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 230000010261 cell growth Effects 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 231100000321 erythema Toxicity 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 239000002023 wood Substances 0.000 description 4
- 229910000906 Bronze Inorganic materials 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 206010030113 Oedema Diseases 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 229940088710 antibiotic agent Drugs 0.000 description 3
- 238000004500 asepsis Methods 0.000 description 3
- 239000010974 bronze Substances 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 239000006229 carbon black Substances 0.000 description 3
- 230000003833 cell viability Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 150000001879 copper Chemical class 0.000 description 3
- KUNSUQLRTQLHQQ-UHFFFAOYSA-N copper tin Chemical compound [Cu].[Sn] KUNSUQLRTQLHQQ-UHFFFAOYSA-N 0.000 description 3
- 210000003746 feather Anatomy 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 238000007654 immersion Methods 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 239000001965 potato dextrose agar Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 2
- 235000017491 Bambusa tulda Nutrition 0.000 description 2
- 241001330002 Bambuseae Species 0.000 description 2
- 241000700199 Cavia porcellus Species 0.000 description 2
- QPLDLSVMHZLSFG-UHFFFAOYSA-N Copper oxide Chemical compound [Cu]=O QPLDLSVMHZLSFG-UHFFFAOYSA-N 0.000 description 2
- 239000005751 Copper oxide Substances 0.000 description 2
- 241000305071 Enterobacterales Species 0.000 description 2
- 206010020751 Hypersensitivity Diseases 0.000 description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 208000026935 allergic disease Diseases 0.000 description 2
- 230000007815 allergy Effects 0.000 description 2
- 230000003373 anti-fouling effect Effects 0.000 description 2
- 230000003260 anti-sepsis Effects 0.000 description 2
- 230000002421 anti-septic effect Effects 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 239000011425 bamboo Substances 0.000 description 2
- 210000001109 blastomere Anatomy 0.000 description 2
- 239000001768 carboxy methyl cellulose Substances 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 229910000431 copper oxide Inorganic materials 0.000 description 2
- 125000004122 cyclic group Chemical group 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000035617 depilation Effects 0.000 description 2
- 229910001873 dinitrogen Inorganic materials 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 239000010985 leather Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000006722 reduction reaction Methods 0.000 description 2
- 229910052709 silver Inorganic materials 0.000 description 2
- 239000004332 silver Substances 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 239000004753 textile Substances 0.000 description 2
- 239000000606 toothpaste Substances 0.000 description 2
- 229940034610 toothpaste Drugs 0.000 description 2
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- WFJIVOKAWHGMBH-UHFFFAOYSA-N 4-hexylbenzene-1,3-diol Chemical compound CCCCCCC1=CC=C(O)C=C1O WFJIVOKAWHGMBH-UHFFFAOYSA-N 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 206010002660 Anoxia Diseases 0.000 description 1
- 241000976983 Anoxia Species 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- VYZAHLCBVHPDDF-UHFFFAOYSA-N Dinitrochlorobenzene Chemical compound [O-][N+](=O)C1=CC=C(Cl)C([N+]([O-])=O)=C1 VYZAHLCBVHPDDF-UHFFFAOYSA-N 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 235000005018 Pinus echinata Nutrition 0.000 description 1
- 241001236219 Pinus echinata Species 0.000 description 1
- 235000017339 Pinus palustris Nutrition 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 230000002547 anomalous effect Effects 0.000 description 1
- 230000007953 anoxia Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 239000003125 aqueous solvent Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010000 carbonizing Methods 0.000 description 1
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- AEJIMXVJZFYIHN-UHFFFAOYSA-N copper;dihydrate Chemical compound O.O.[Cu] AEJIMXVJZFYIHN-UHFFFAOYSA-N 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 239000000890 drug combination Substances 0.000 description 1
- 230000002497 edematous effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000002082 metal nanoparticle Substances 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 238000007655 standard test method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Images
Abstract
The invention discloses an application of a copper nanomaterial with a copper/carbon-core/shell structure in antibiosis, and especially in bacteriostasis and/or sterilization for fungi or bacteria. The nanomaterial with the copper/carbon-core/shell structure has stable material performance, is harmless to human body, is environment-friendly and ecology-friendly, and is an antibiotic material with a broad spectrum. Because of the function characteristics and the special sterilization mechanism of the copper nanomaterial, drug resistance is difficult to generate by various bacteria, and the advantage is incomparable to other biochemical antibiotic medicine.
Description
Technical field
The invention belongs to the anti-biotic material technical field, be specifically related to have copper/carbon-the copper nano material of core/shell structure is in the application of antibiosis.
Background technology
The copper nano particles of copper/carbon-core/shell structure is to be template with the vegetable fiber, and metal copper ion is a raw material, (the Patent Application No. that connects the Kon professor: PCT/US07/61862) novel nano granular materials that is prepared from through the carbonizing reduction method of heating.Existing copper nano particles material is compared following property on this copper/carbon-nuclear/core-shell nanoparticles material and the market: (1) has the film formed shell structure of reticulated carbon of property, and it not only can protect nanometer copper nuclear to avoid the excessive erosion of external environment but also can let the characteristic present of nanometer copper come out; (2) copper nano particles of this core/shell structure is very stable in surroundings, can keep the form of metallic copper for a long time; (3) in oxidation environment, generate the nano particle equilibrium system of copper=cuprous oxide, do not form copper oxide; (4) nano particle that in oxidation environment, generates copper=cuprous oxide converts metallic copper again to when running into reducing condition, and keeps original nuclear/core-shell nanoparticles structure; (5) prepared material is to exist with the form that nano copper particle is embedded in the porous carbon black matrix equably; Its physical size scope can according to the actual process needs the hundreds of nanometer to several millimeters in addition decimeter between change (can give full play to the advantage of nano material, can avoid nano-particle material again) usually because the too little unmanageable shortcoming of size; (6) this copper nano particles material does not dissolve medium-term and long-term immersion of the aqueous solution of pH value 1-11, does not have tangible copper ion to ooze out in the solution; (7) netted carbon shell structure has kept functional groups such as original hydroxyl of cellulose and carbonyl, and prepared nano particle all has good dispersiveness and intersolubility in polar solvent (like water) and non-polar organic solutions (like oil).
Summary of the invention
The object of the present invention is to provide to have copper/carbon-the copper nano material of core/shell structure is in the application of antibiosis, can be applicable to aspects such as antibiosis, anticorrosion and sterilization.
For achieving the above object, this is that the technical scheme that adopts is:
The application of antibacterial and/or sterilization with nano material of copper/carbon-core/shell structure.
The application of the antibacterial and/or sterilization of described nano material with copper/carbon-core/shell structure is characterized in that: be the antibacterial and/or sterilization to fungi or bacterium.
The application of the antibacterial and/or sterilization of described nano material with copper/carbon-core/shell structure, it is characterized in that: described fungi is Bipolaris fungi (Bipolar mildew fungi), bipolaris fungi (Helminthosporium fungi), fungus Trichoderma (Trichoderma fungi), white-rot fungi (White rot fungi) or blue stain fungi (Blue stain fungus).
The application of the antibacterial and/or sterilization of described nano material with copper/carbon-core/shell structure, it is characterized in that: described bacterium is Escherichia coli (Escherichia coli), gold-coloured staphylococci (Staphylococcus aureus) or Candida albicans (Candida albicans).
Compared with prior art, the present invention has following beneficial technical effects:
Nano material with copper/carbon-core/shell structure is water insoluble, does not in use almost have the copper ion stripping, and the not diffusion everywhere with flowing of water can be medium-term and long-term not showy at air, has self-repair function, and the antibacterial effect phase is long; Harmless when routine is used, environmental friendliness, ecological friendly, be easy to control, low price, the broad-spectrum antiseptic material of contact killing type.
Cyclic oxidation-reduction reaction with nano material peculiar " copper-cuprous oxide-copper " of copper/carbon-core/shell structure can relate to continuous electron exchange.The exchange reaction of these electronics can produce the growth of microbial cell and disturb just, and then draws up, and destroys the growth of cell, plays antibiotic and effect sterilization.Because the functional characteristic of this copper metal nanoparticle and the special mechanism of sterilization thereof, this just makes that various mushrooms are difficult to it is developed immunity to drugs, and this advantage is that other biochemical type of antimicrobial institute is incomparable.
Compare with traditional anti-biotic material-Nano Silver, copper has 0 valency, 1 valency and three kinds of states of divalent, and the electronic switch process is arranged in course of reaction twice, has the antibiosis that is superior to silver, anticorrosion and the function of sterilizing, and has bigger price advantage.
Nano material with copper/carbon-core/shell structure not only can be processed formulations such as solvent, gel, also can sneak into plastic products, coating; Composite, toothpaste, textile, leather and fur products;, antifouling in the different products such as bamboo and wood products as antibiotic, mildew-resistant, anticorrosive additive.
Description of drawings
Fig. 1 is that the copper nano particles with copper/carbon-core/shell structure carries out continuous 4 times and repeats heating differential analysis figure from 40 ℃ to 500 ℃ under condition of nitrogen gas;
Fig. 2 is the heating differential analysis result of copper nano particles under excess oxygen with copper/carbon-core/shell structure;
Fig. 3-a～Fig. 3-b is with the anti-Bipolaris of nano material that contains variable concentrations (0.15%, 0.3%) copper/carbon-core/shell structure/observed result of 72,240 hours of bipolaris fungi experiment;
Fig. 4-a～Fig. 4-b is the contrast (40 *) that Bipolaris/bipolaris fungi is not containing/containing nano material cultivation displaing micro picture of culture experiment after 720 hours of copper/carbon-core/shell structure;
Fig. 5 is the antibiotic cultivation results of the copper nano particles of copper/carbon-core/shell structure to fungus Trichoderma;
Fig. 6 is the antibiotic cultivation results of the copper nano particles of copper/carbon-core/shell structure to white-rot fungi;
Fig. 7 is the antibiotic cultivation results of the copper nano particles of copper/carbon-core/shell structure to the blue stain fungi.
Embodiment
Below in conjunction with concrete embodiment and accompanying drawing the present invention is done further detailed description, said is to explanation of the present invention rather than qualification.
The nano particle of described copper/carbon-core/shell structure is the (Patent Application No. that connects the Kon professor: the PCT/US07/61862) nano particle of the copper/carbon-core/shell structure of preparation.Its major parameter is following:
1) nano particle of copper/carbon-core/shell structure, the percentage by weight of copper are 25%, and remaining is the porous carbon black;
2) nano particle all is the compound that inlays equably in the porous carbon black, and used copper nano particles material is of a size of average diameter at 15 microns the porous charcoal black-materials of inlaying a plurality of copper nano particles.Used dusty material is placed in the aqueous solution after ultrasonic agitation placed 24 hours, with the laser diffraction particle calculating instrument upper solution is detected, the result shows not have the nano particle existence that dissociates.
3) copper nano particles both can distribute and polarizable medium (like aqueous solvent etc.) equably, also can distribute equably with polarizable medium (like oil-based solvent, latex, materials such as plastics) in.
And the nano particle of prepared copper/carbon-core/shell structure carried out following stability test:
1) stability experiment of the nano particle of copper/carbon-core/shell structure in solution:
With the nano particle of the copper/carbon-core/shell structure of preparation is that (acid and alkaline solution adds sulfuric acid (H with deionized water respectively for 1,4,5,6,7,8,9 and 11 solution at pH respectively
2SO
4) or sodium hydroxide (NaOH) preparing solution form) in carried out 60 days immersion test by a definite date.Because the concentration of electrical conductivity and copper ion is directly proportional, in experiment, detect the change in concentration of copper ion in the solution with the variation of electrical conductivity.If copper ion is stripping from nano particle, the electrical conductivity of solution can correspondingly increase.The result shows the immersion of passing through 60 days, and the electrical conductivity of solution does not have marked change, explains that nano copper particle does not have the tendency of dissolving in pH is 1,4,5,6,7,8,9 and 11 solution.
2) heat endurance of the nano particle of copper/carbon-core/shell structure
The nano particle of metal/carbon-core/shell structure has extraordinary heat endurance.Nano particle with copper/carbon-core/shell structure is an example, it has been carried out continuous 4 times repeat heating differential analysis from 40 ℃ to 500 ℃ under condition of nitrogen gas.Its result shows (Fig. 1) under anoxia condition, and the nano particle of copper/carbon-core/shell structure below 500 ℃ all is being stable (not reacting the peak occurs).When heating (aerobic environment) at air conditions, the heating differential analysis result shows that the nano particle of (Fig. 2) copper/carbon-core/shell structure all is being stable below 280 ℃.
Antibacterial action with copper nano material of copper/carbon-core/shell structure
With respect to traditional anti-biotic material-Nano Silver, copper has 0 valency, 1 valency and three kinds of states of divalent, and the electronic switch process is arranged in course of reaction twice, should have the antibiosis that is superior to silver, anticorrosion and the function of sterilizing in theory, and have bigger price advantage.But because existing nano copper particle is extremely unstable in environment, can all be oxidized to divalent copper (copper oxide or Kocide SD) in the short time, then lose its effect basically.It is documented to show with experimental result that in the compound of copper, 1 valency copper (cuprous oxide) has the strongest antibiosis, anticorrosion, sterilization and the effect of sterilizing.
The nano particle of copper/carbon-core/shell structure owing to its unique shell structure, can keep the chemical state of metallic copper for a long time in common environment.Under oxidizing condition, nano copper particle can form the equilibrium system of copper-cuprous oxide (1 valency copper), can not generate cupric.When running into reducing environment (many bacterial growths can produce reducing environment), the cuprous oxide in copper-cuprous oxide system can reduce again becomes zerovalent copper.Cyclic oxidation-the reduction reaction of the nano particle of this copper/carbon-core/shell structure peculiar " copper-cuprous oxide-copper " can relate to continuous electron exchange.The exchange reaction of these electronics can produce the growth of microbial cell and disturb just, and then draws up, and destroys the growth of cell, plays antibiotic and effect sterilization.Add because cuprous oxide is water insoluble, avoided the dissolving of nano copper particle in water, not only prevented the pollution of copper ion, also guaranteed the durability of copper nano particles environment.
The broad-spectrum antibacterial action of the nano material of copper/carbon-core/shell structure
1) anti-Bipolaris/bipolaris fungi
The medium (the copper nano particles compounds of 3.9 gram potato dextrose agar powder+100 ml distilled waters+different proportions) that is respectively 0.15%, 0.3% and 0 (control group) with the nano material mass percent concentration of cupric/carbon-core/shell structure carries out the cultivation of Bipolaris and two types of fungies of bipolaris fungi.After inoculation, put into temperature and be 30 ℃ insulating box and cultivate.Sample was put into aseptic laminar flow case and is detected also film recording in the time of 24,72,144 and 240 hours.
Cultivation results shown in Fig. 3-a (0.15%w), Fig. 3-b (0.3%w) (left side is the bipolaris fungi for Bipolaris fungi, right side among the figure); In containing the nano-particle complex particle of copper/carbon of the 0.15%w-core/shell structure medium of (weight ratio of cupric is about 8/10000ths); Size with colony growth is estimated, and Bipolaris/bipolaris fungi growth speed is compared obvious decline with control group; And nano-particle complex particle (weight ratio of cupric is about 16/10000ths) Bipolaris/bipolaris fungi growth speed that contains copper/carbon-core/shell structure of 0.3%w is zero.
Pass through microscopic; When the content that Bipolaris/bipolaris fungi is inoculated in the cupric nano-particle complex reaches the medium of 0.3%w, after 204 hours, there is not the generation of mycoderm at the edge in inoculation district; The mycelia drying shrinkage of the bacterium colony of implanting during inoculation; The metabolism of bacterium colony stops, and does not have conidium to generate, and bacterium colony is in dead state.
Like Fig. 4-a (normal cultured; As contrast), the micro-view contrast (40 *) shown in Fig. 4-b (nano material of 0.3% bronze medal/carbon-core/shell structure); The metabolism of mycelia of (left figure) Bipolaris/bipolaris fungi and conidium is normal in the control sample; The conidium growth is vigorous, makes bacterium colony constantly grow up; When cupric nano material compound is 0.3%w (right figure), the mycelia drying shrinkage does not have visible conidium, and bacterium colony is in dead state.
The nano-particle complex that above result shows copper/carbon-core/shell structure has the obvious suppression effect to Bipolaris/bipolaris fungi (0.1%w) when the low concentration; (0.3%w) demonstrates effective sterilizing function when concentration reaches certain value.
2) ability of anti-fungus Trichoderma:
The nano-particle complex weight percent concentration of using cupric/carbon-core/shell structure is the culture experiment that the medium (the copper nano particles compounds of 3.9 gram potato dextrose agar powder+100 ml distilled waters+different proportions) of 0.03% and 0 (control group) carries out fungus Trichoderma; After inoculation, put into temperature and be 30 ℃ insulating box and cultivate.Sample was put into aseptic laminar flow case and is detected also film recording in the time of 24,48 and 72 hours.
The result is as shown in Figure 5: the fungus Trichoderma growth of control group is normal, and bacterium colony increases rapidly; And when the nano copper composition particle that contains 0.03%w in medium when (weight ratio of cupric is about 5/10000ths), the growth that fungus Trichoderma is given birth to stops, and whole bacterium colony is in dead state.Therefore visible, even when concentration is 0.03%w, life has good sterilization functions to the copper nano particles compound to fungus Trichoderma.
3) ability of anti-white-rot fungi
Using the cupric percentage by weight is that the nano-particle complex particle of copper/carbon-core/shell structure of 7/10000ths has carried out the wood corrosion experiment of whiterot fungi, and concrete the installation by american wood protects the AWPAE0 standard test method of association (AWPA) to carry out.Sample is the southern pine of the U.S., after the copper nano particles composite is handled, sample on average contain 7/10000ths copper nano particles composite.
Experimental result is as shown in Figure 6, and the sample of control group (right figure) white-rot fungi after 600 hours has covered whole specimen surface.Then do not receive the invasion and attack of white-rot fungi fully through sample (left figure) surface that the copper nano particles composite was handled.Experimental result shows that the nano-particle complex that contains the copper/carbon-core/shell structure of 7/10000ths bronze medals has effective sterilization antisepsis to white-rot fungi.
4) ability of anti-blue stain fungi:
The nano-particle complex particle that is respectively copper/carbon-core/shell structure of 0.01%, 0.05%, 0.1%, 0.25% and 0.5% with the cupric percentage by weight carries out the culture experiment (the copper nano particles compounds of 3.9 gram potato dextrose agar powder+100 ml distilled waters+different proportions) of blue stain fungi.
Show that through 25 days experimental results (Fig. 7) the blue stain fungi growth is unaffected basically when the content of copper nano particles composite is 0 to 0.1%w; But when the content of copper nano particles composite reaches 0.25%w; The blue stain fungi growth receives obvious suppression; The bacterium colony size obviously diminishes, and shows that the nano-particle complex of the copper/carbon-core/shell structure that contains 7/10000ths bronze medals has effective sterilization antisepsis to white-rot fungi.
5) ability of Chinese People's Anti-Japanese Military and Political College enterobacteria, gold-coloured staphylococci and Candida albicans:
The nano-particle complex of cupric/carbon-core/shell structure is that the sample of dry weight 5/1000ths has carried out the experiment of Chinese People's Anti-Japanese Military and Political College enterobacteria, staphylococcus aureus and Candida albicans in the disease prevention and control center, Shaanxi Province according to " disposable use amenities sanitary standard ".The neutralizer approval test adopts the suspension method approval test, and to staphylococcus aureus (ATCC6538) effect 1min, test temperature is 20 ± 2 ℃, and relative moisture is 50%.Quantitative disinfecting test is the suspension method test.Test temperature is 20 ± 2 ℃, and relative moisture is 50%.The test triplicate.
Experimental result shows that this sample has good bactericidal effect.The PBS solution that contains 3.0% Tween-80+0.3% lecithin can neutralize to 0.5% sample that contains nano copper particle fully, and this neutralizer product does not all have influence to staphylococcus aureus (ATCC6538), Candida albicans (ATCC10231), and result of the test is seen table 1.
Table 1 neutralizer approval test result
In test temperature is under 20 ± 2 ℃ of conditions, and submitted sample contains 0.5% mass action 1min of nano copper particle, and the average bactericidal rate of staphylococcus aureus (ATCC6538) is reached more than 90%; In test temperature is under 20 ± 2 ℃ of conditions, and submitted sample contains 0.5% mass action 1min of nano copper particle, and the average bactericidal rate of Escherichia coli (8099) is reached more than 90%; In test temperature is under 20 ± 2 ℃ of conditions, and submitted sample contains 0.5% mass action 1min of nano copper particle, and the average bactericidal rate of Candida albicans (ATCC10231) is reached more than 90%.The result sees table 2, table 3, table 4.
Table 2 copper core-shell nano particle is to the bactericidal effect of staphylococcus aureus
Annotate: the average clump count of positive controls is 3.95 * 10
4Cfu/mL; The negative control group asepsis growth
Table 3 copper core-shell nano particle is to colibacillary bactericidal effect
Annotate: the average clump count of positive controls is 4.35 * 10
4Cfu/mL; The negative control group asepsis growth
Table 4 copper core-shell nano particle is to the oidiomycetic bactericidal effect of white
Annotate: the average clump count of positive controls is 5.01 * 10
4Cfu/mL; The negative control group asepsis growth
The safety of the nano material of copper/carbon-core/shell structure
1) nanometer copper is to the stimulation test of skin:
The nano-particle complex of copper/carbon-core/shell structure is carried out zoopery to the effect of stimulation of skin: during experiment, get 10 of healthy regular grade NZws, be divided into three groups, A group working concentration is the nano copper particle compound of 2% weight ratio; B group working concentration is the nano copper particle compound of 5% weight ratio; The C group uses the carboxymethyl cellulose-based matter that does not contain nanometer copper as the blank group.
Tame rabbit back is lost hair or feathers with hair remover, and the depilation area is 150cm
2, treat to clean up with warm water after 2～3 minutes, till visible bright and clean skin.Whether the inspection skin of unhairing is because of the unhairing damaged after 24 hours, and compromised skin is unfit to do the irritant test of intact skin.Clean gauze is immersed in to take out behind the different solution applies at the rabbit back, divide cage to feed, remove gauze after 24 hours and use the distilled water flush away, observation topical application of drug position, unhairing district has or not symptoms such as erythema and oedema in 1,24,48, in the 72h.The rabbit skin of handling with 2% and 5% your nano copper particle compound in observation period does not see that ANOMALOUS VARIATIONS is arranged, and show is not swollen to smear the back of being tried thing, festers reactions such as inflammation necrosis.
This experiment shows that the kind concentration of working as the nano copper particle compound below 5%, does not have toxicity and excitant to skin.
2) nanometer copper is tested the sensitization of skin:
Sensitization of skin experiment is the nano copper particle compound and 2 with 2% and 5% weight ratio, and 4-dinitrochlorobenzene (DNCB) (positive control) is scattered in and carries out on one's body the SD rat in the excipient of 5%w sodium carboxymethylcellulose.
Preceding 1 day of experiment to the mouse left side with hair remover processings of losing hair or feathers, preceding 1 day of sensitization is carried out cropping to the right side, and then loses hair or feathers.Get each one milliliter of excipient, positive drug, 2% and 5% nano copper particle complex formulation during sensitization respectively and be applied to above depilation place of respectively organizing cavy left side skin, bandage fixing then; Remove the drug combination warm water cleaning behind the 6h, observe in 1h and 24h time point, and give a mark according to " guideline "; 7th, repeated in 14 days with quadrat method administration and observation; The 28th day in the right side administration excites, observe and same marking (experiment amounts to about 28 days of fate).
During sensitization, the guinea pig skin of vehicle control group was not met quick reaction; The nano copper particle compound is tried the individual animal skin of drug group and is seen that a little erythema is arranged; Positive drug treated animal skin is seen has slight oedema.After exciting, the vehicle control group with tried drug group and seen have a little erythema except that guinea pig skin out of the ordinary, all do not see other abnormal responses; Positive drug treated animal skin sees that obvious oedema and edematous erythema are arranged, and has skin to decrease appearance mostly.
The sensitization of skin experimental result shows: vehicle control group and nano copper particle compound are tried drug group allergy incidence of 24h and 48h after sensitization and are zero; Positive drug group allergy incidence of 24h and 48h after sensitization is 100%.The nano copper particle compound does not have sensitization below 5% weight ratio concentration.
3) nano particle of copper/carbon-core/shell structure is to the influence of people's kidney blastocyte strain HEK-293 cells survival rate:
The toxicity test of nano copper particle compound is to measure cell viability through its influence to people's kidney blastocyte strain HEK-293 cells survival rate with mtt assay to estimate.In the control group, the survival rate was 100%, when the concentration of nano-Cu 5 × 10
-7 ~ 5 × 10
-2 mg / mL range, the cell viability at 95.47 ± 9.17% to 102.94 ± 4.43%, seen at this concentration range, Pei copper on human kidney cell line HEK-293 cell growth was not inhibited; when the nano Cu concentration of 5 × 10-1mg/mL, the cell viability dropped 67.05052 ± 14.4601%, the concentration of copper on HEK-293 cell growth inhibition.
Comprehensive above the analysis, the result shows to have copper/carbon-the nano material stable in properties of core/shell structure, and is harmless, environmental friendliness is ecological friendly, is a kind of broad-spectrum antiseptic material.Nano material with copper/carbon-core/shell structure not only can be processed formulations such as solvent, gel, also can sneak into plastic products, coating; Composite, toothpaste, textile, leather and fur products;, antifouling in the different products such as bamboo and wood products as antibiotic, mildew-resistant, anticorrosive additive.
Claims (4)
1. the application of antibacterial and/or sterilization that has the nano material of copper/carbon-core/shell structure.
2. the application of the antibacterial and/or sterilization of the nano material with copper/carbon-core/shell structure as claimed in claim 1 is characterized in that: be the antibacterial and/or sterilization to fungi or bacterium.
3. the application of the antibacterial and/or sterilization of the nano material with copper/carbon-core/shell structure as claimed in claim 2, it is characterized in that: described fungi is Bipolaris fungi (Bipolar mildew fungi), bipolaris fungi (Helminthosporium fungi), fungus Trichoderma (Trichoderma fungi), white-rot fungi (White rot fungi) or blue stain fungi (Blue stain fungus).
4. the application of the antibacterial and/or sterilization of the nano material with copper/carbon-core/shell structure as claimed in claim 2, it is characterized in that: described bacterium is Escherichia coli (Escherichia coli), gold-coloured staphylococci (Staphylococcus aureus) or Candida albicans (Candida albicans).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110308477.2A CN102499260B (en) | 2011-10-12 | 2011-10-12 | Application of copper nanomaterial with copper/carbon-core/shell structure in antibiosis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110308477.2A CN102499260B (en) | 2011-10-12 | 2011-10-12 | Application of copper nanomaterial with copper/carbon-core/shell structure in antibiosis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102499260A true CN102499260A (en) | 2012-06-20 |
| CN102499260B CN102499260B (en) | 2014-12-10 |
Family
ID=46211442
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110308477.2A Active CN102499260B (en) | 2011-10-12 | 2011-10-12 | Application of copper nanomaterial with copper/carbon-core/shell structure in antibiosis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102499260B (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340965A (en) * | 2015-10-28 | 2016-02-24 | 连崑 | Application of copper-carbon nanocomposite to algae control |
| CN106277473A (en) * | 2016-09-29 | 2017-01-04 | 西安交通大学苏州研究院 | A kind of water purifying cup with antibacterial filter element |
| CN108395680A (en) * | 2017-09-22 | 2018-08-14 | 浙江中迪纺织新材料科技有限公司 | A kind of tablecloth of the polyester antibacterial plastic concentrate based on nanometer Cu/C composite materials |
| CN108410139A (en) * | 2017-09-22 | 2018-08-17 | 浙江中迪纺织新材料科技有限公司 | A kind of high molecular polymer antibacterial plastic concentrate and preparation method thereof based on nanometer Cu/C composite materials |
| CN108653240A (en) * | 2018-06-22 | 2018-10-16 | 苏州冠洁纳米抗菌涂料科技有限公司 | The application of the composite nanoparticle of carbon and copper |
| CN112341712A (en) * | 2019-08-09 | 2021-02-09 | 安徽银源环保科技有限公司 | Copper-carbon antibacterial water delivery pipe, microbead copper-carbon antibacterial water delivery pipe and preparation method thereof |
| CN112356368A (en) * | 2020-10-14 | 2021-02-12 | 上海那博新材料科技有限公司 | Production process of nano-copper latex pillow |
| CN112522861A (en) * | 2020-10-12 | 2021-03-19 | 苏州冠洁纳米材料科技有限公司 | Application of composite nano particles of carbon and copper in melt-blown non-woven material |
| CN113068714A (en) * | 2021-04-01 | 2021-07-06 | 联科华技术有限公司 | Monoatomic copper preparation capable of replacing traditional agricultural copper preparation and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101143326A (en) * | 2007-10-09 | 2008-03-19 | 河北大学 | Preparation method and application of a compound carrier metal nanometer catalyst |
| CN101209493A (en) * | 2006-12-27 | 2008-07-02 | 上海海联润滑材料科技有限公司 | Nano core-shell type copper-nickel bimetal powder body and preparing method and application thereof |
| CN101224434A (en) * | 2007-12-29 | 2008-07-23 | 中国科学院长春应用化学研究所 | Preparing method of nanometer particle carbon nanotube compound catalyst |
-
2011
- 2011-10-12 CN CN201110308477.2A patent/CN102499260B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101209493A (en) * | 2006-12-27 | 2008-07-02 | 上海海联润滑材料科技有限公司 | Nano core-shell type copper-nickel bimetal powder body and preparing method and application thereof |
| CN101143326A (en) * | 2007-10-09 | 2008-03-19 | 河北大学 | Preparation method and application of a compound carrier metal nanometer catalyst |
| CN101224434A (en) * | 2007-12-29 | 2008-07-23 | 中国科学院长春应用化学研究所 | Preparing method of nanometer particle carbon nanotube compound catalyst |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340965A (en) * | 2015-10-28 | 2016-02-24 | 连崑 | Application of copper-carbon nanocomposite to algae control |
| WO2017071108A1 (en) * | 2015-10-28 | 2017-05-04 | 连崑 | Use of copper-carbon composite nanomaterial in algae control |
| CN106277473A (en) * | 2016-09-29 | 2017-01-04 | 西安交通大学苏州研究院 | A kind of water purifying cup with antibacterial filter element |
| CN108395680A (en) * | 2017-09-22 | 2018-08-14 | 浙江中迪纺织新材料科技有限公司 | A kind of tablecloth of the polyester antibacterial plastic concentrate based on nanometer Cu/C composite materials |
| CN108410139A (en) * | 2017-09-22 | 2018-08-17 | 浙江中迪纺织新材料科技有限公司 | A kind of high molecular polymer antibacterial plastic concentrate and preparation method thereof based on nanometer Cu/C composite materials |
| CN108653240A (en) * | 2018-06-22 | 2018-10-16 | 苏州冠洁纳米抗菌涂料科技有限公司 | The application of the composite nanoparticle of carbon and copper |
| CN108653240B (en) * | 2018-06-22 | 2021-03-02 | 苏州冠洁纳米材料科技有限公司 | Application of composite nano-particle of carbon and copper |
| EP3811934A4 (en) * | 2018-06-22 | 2022-06-08 | Suzhou Guanjie Nano Materials Technology Co., Ltd. | Use of composite nanoparticle of carbon and copper |
| CN112341712A (en) * | 2019-08-09 | 2021-02-09 | 安徽银源环保科技有限公司 | Copper-carbon antibacterial water delivery pipe, microbead copper-carbon antibacterial water delivery pipe and preparation method thereof |
| CN112522861A (en) * | 2020-10-12 | 2021-03-19 | 苏州冠洁纳米材料科技有限公司 | Application of composite nano particles of carbon and copper in melt-blown non-woven material |
| CN112356368A (en) * | 2020-10-14 | 2021-02-12 | 上海那博新材料科技有限公司 | Production process of nano-copper latex pillow |
| CN113068714A (en) * | 2021-04-01 | 2021-07-06 | 联科华技术有限公司 | Monoatomic copper preparation capable of replacing traditional agricultural copper preparation and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102499260B (en) | 2014-12-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102499260B (en) | Application of copper nanomaterial with copper/carbon-core/shell structure in antibiosis | |
| Shaheen et al. | Integration of cotton fabrics with biosynthesized CuO nanoparticles for bactericidal activity in the terms of their cytotoxicity assessment | |
| Abo-Shama et al. | Synergistic and antagonistic effects of metal nanoparticles in combination with antibiotics against some reference strains of pathogenic microorganisms | |
| Yousef et al. | In vitro antibacterial activity and minimum inhibitory concentration of zinc oxide and nano-particle zinc oxide against pathogenic strains | |
| Sadeghi et al. | Comparison of the anti-bacterial activity on the nanosilver shapes: nanoparticles, nanorods and nanoplates | |
| Seo et al. | Antibacterial activity and cytotoxicity of multi-walled carbon nanotubes decorated with silver nanoparticles | |
| Xu et al. | Role of reactive oxygen species in the antibacterial mechanism of silver nanoparticles on Escherichia coli O157: H7 | |
| Mahmoodabadi et al. | Characterization, antibacterial and cytotoxicity studies of graphene-Fe3O4 nanocomposites and Fe3O4 nanoparticles synthesized by a facile solvothermal method | |
| Song et al. | Fabrication of silver nanoparticles and their antimicrobial mechanisms | |
| Hoseinzadeh et al. | Antimicrobial potential of synthesized zinc oxide nanoparticles against gram positive and gram negative bacteria | |
| Sadeghi et al. | Synthesis and characterization of silver nanoparticles for antibacterial activity | |
| Cano et al. | Copper sulfate-embedded and copper oxide-embedded filter paper and their antimicrobial properties | |
| JP2012526777A (en) | Biocide Nanostructured Composition and Method for Obtaining Nanostructured Biocide Composition | |
| Parandhaman et al. | Facile synthesis, biofilm disruption properties and biocompatibility study of a poly-cationic peptide functionalized graphene–silver nanocomposite | |
| Shahrokh et al. | Toxicity and unusual biological behavior of nanosilver on gram positive and negative bacteria assayed by microtiter-plate | |
| Zholobak et al. | Interaction of nanoceria with microorganisms | |
| Anwar et al. | Comparison of antibacterial activity of Ag nanoparticles synthesized from leaf extract of Parthenium hystrophorus L in aqueous media and gentamicin sulphate: in-vitro | |
| Ghorbi et al. | Influence of nano-copper oxide concentration on bactericidal properties of silver–copper oxide nanocomposite | |
| He et al. | Facile preparation of L-ascorbic acid-stabilized copper-chitosan nanocomposites with high stability and antimicrobial properties | |
| Obaid et al. | Study the effect of antibacterial on the chemically prepared copper oxide | |
| Gupta et al. | Comparative assessment of antibacterial efficacy for cobalt nanoparticles, bulk cobalt and standard antibiotics: a concentration dependant study | |
| Verma | A review on synthesis and their antibacterial activity of Silver and Selenium nanoparticles against biofilm forming Staphylococcus aureus | |
| Pohle et al. | Antimicrobial properties of orthopaedic textiles after in-situ deposition of silver nanoparticles | |
| Maliha et al. | Bismuth phosphinato incorporated antibacterial filter paper for drinking water disinfection | |
| Chaitanyakumar et al. | Biogenically engineered silver nanoparticles using bael leaf extract and evaluation of its therapeutic potential |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Lian Kun Inventor after: Li Weifeng Inventor after: Wang Xiaopeng Inventor after: Niu Xiaofeng Inventor after: Wu Qinglin Inventor after: Qi Yadong Inventor after: Chen Tianning Inventor after: Chen Hualing Inventor before: Li Weifeng Inventor before: Lian Kun Inventor before: Wang Xiaopeng Inventor before: Niu Xiaofeng Inventor before: Wu Qinglin Inventor before: Qi Yadong Inventor before: Chen Tianning Inventor before: Chen Hualing |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: LI WEIFENG LIAN KUN WANG XIAOPENG NIU XIAOFENG WU QINGLIN QI YADONG CHEN TIANNING CHEN HUALING TO: LIAN KUN LI WEIFENG WANG XIAOPENG NIU XIAOFENG WU QINGLIN QI YADONG CHEN TIANNING CHEN HUALING |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160315 Address after: 215123, C315, 99 benevolence Road, Suzhou Industrial Park, Jiangsu, Suzhou Patentee after: Suzhou Jie Jie nano antibacterial coating technology Co., Ltd. Address before: 710049 Xianning West Road, Shaanxi, China, No. 28, No. Patentee before: Xi'an Jiaotong University |
|
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: Room 207, building 1, No.168 Yuxin Road, Suzhou Industrial Park, Suzhou area, China (Jiangsu) pilot Free Trade Zone, Suzhou City, Jiangsu Province Patentee after: Suzhou Guanjie nano material technology Co., Ltd Address before: 215123, C315, 99 benevolence Road, Suzhou Industrial Park, Jiangsu, Suzhou Patentee before: SUZHOU GUANJIE NANO ANTIBACTERIAL COATING TECHNOLOGY Co.,Ltd. |