CN102495035A - Quick and high-efficiency fluorescence detection method for phosphate ions - Google Patents

Quick and high-efficiency fluorescence detection method for phosphate ions Download PDF

Info

Publication number
CN102495035A
CN102495035A CN2011103993309A CN201110399330A CN102495035A CN 102495035 A CN102495035 A CN 102495035A CN 2011103993309 A CN2011103993309 A CN 2011103993309A CN 201110399330 A CN201110399330 A CN 201110399330A CN 102495035 A CN102495035 A CN 102495035A
Authority
CN
China
Prior art keywords
phosphate anion
detection method
solution
fluorescence
phosphate ions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2011103993309A
Other languages
Chinese (zh)
Inventor
王光丽
焦焕军
董玉明
朱晓瑛
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangnan University
Original Assignee
Jiangnan University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangnan University filed Critical Jiangnan University
Priority to CN2011103993309A priority Critical patent/CN102495035A/en
Publication of CN102495035A publication Critical patent/CN102495035A/en
Pending legal-status Critical Current

Links

Images

Landscapes

  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The invention provides a quick and high-efficiency fluorescence detection method for phosphate ions. Rare earth ions can quench fluorescence based on a quantum dot, and the fluorescence with the phosphate ions can be recovered effectively. Compared with the most common fluorescence quenching type probe based on the quantum dot, a novel off-on type quantum dot fluorescence probe can effectively prevent the fluorescence from being quenched due to other factors in a solution, so that the measurement selectivity is improved greatly. As known, the method is the first application of the quantum dot to measurement of the phosphate ions. According to the method, the phosphate ions can be measured with high sensitivity; the linear range is 1*10<-7> to 5*10<-6> mol/L; and the detection limit is 5*10<-8> mol/L. Common anions do not have fluorescence response signals, so that the method is high in selectivity; a satisfying result of measurement of the phosphate ions in an analog water sample is obtained; and the method is expected to be applied to detection of the phosphate ions in a water body.

Description

Quick, the high-efficiency fluorescence detection method of phosphate anion
Technical field:
The present invention relates to the analyzing and testing field, relate in particular to the synthetic method and the application aspect the phosphate anion in the fluoroscopic examination water body thereof of the quantum dot optics probe of water-soluble, high stability.
Background technology:
Phosphate anion plays a part very important [P.D.Beer and E.J.Hayes, Coord.Chem.Rev., 2003,240,167-189.] in numerous relating in life, environment and the chemical process.Articles for washing and farmland fertilization all can produce the discharging of phosphate anion in people's daily life, cause that the content of phosphate radical constantly increases in the natural water body.But the too high (＞0.2mgL of phosphorus content in the water -1), then can make the water nutrition enrichment, cause the excessive multiplication of algae and other hydrophyte public hazards, cause water quality deterioration; Bring peculiar smell to water body, cause environmental pollution [H.P.Jarvie, C.Neal; P.J.A.Withers, A.Robinson and N.Salter, Hydrol.Earth Syst.Sci.; 2003,7,722-743; P.Stalnacke, S.M.Vandsemb, A.Vassiljev, A.Grimvall and G.Jolankal, Water Sci.Technol., 2004,49,29-36; P.W.Balls, A.Macdonald, K.Pugh and A.C.Edwards, Environ.Pollut., 1995,90,311-321].And caused pollution to environment, therefore, the detection of phosphate anion has realistic meaning in the environment.The assay method of the phosphate anion of report mainly contains AAS [F.Pena-Pereira, N.Cabaleiro, I.de la Calle, M.Costas, S.Gil, I.Lavilla and C.Bendicho.Talanta, 2011,85,1100-1104 at present; C.M.Li, Y.F.Li, J.Wang and C.Z.Huang.Talanta, 2010,81; 1339-1345], fluorescence method [Y.Udnan, I.D.McKelvie, M.R.Grace, J.Jakmunee and K.Grudpan.Talanta, 2005; 66,461-466], chromatography [J.B.Quintana, R.Rodil and T.Reemtsma.Anal.Chem., 2006; 78,1644-1650], electrochemical process [W.L.Cheng, J.W.Sue, W.C.Chen; J.L.Chang and J.M.Zen.Anal.Chem., 2010,82,1157-1161; L.Gilbert, A.T.A.Jenkins, S.Browning and J.P.Hart.Anal.Biochem., 2009,393,242-247], ICP-MS methods such as [Z.X.Guo, Q.Cai and Z.Yang.J.Chromatogr.A, 2005,1100,160-167].But these methods are often more consuming time, and often have comparatively serious disturbance phenomenon.
Semi-conductor nano particles (claiming quantum dot again), have uniqueness and excellent optical property like wide excitation spectrum, narrow emission spectrum, emission wavelength and relevant, the excellent [A.P.Alivisatos such as anti-photobleaching property of the particle diameter of nano particle; Science; 1996,271,933-937].Therefore, quantum dot has received extensive concern, is a kind of fluorescence probe [J.M.Klostranec and W.C.W.Chan.Adv.Mater., 2006,18,1953-1964 that is hopeful very much to be substituted with organic dye; M.J.Bruchez, M.Moronne and A.P.Alivisatos.Science, 1998,281,2013-2016].Functional quantum dot detects Hg as optical probe fluorescence method method 2+, Pb 2+, Cu 2+[Y.F.Chen and R.Z.Zeev, Anal.Chem., 2002,7,5132-5138; Z.X.Cai, H.Yang, Y.Zhang and X.P.Yan, Anal.Chim.Acta, 2006,559,234-239; G.L.Wang, Y.M.Dong and Z.J.Li, Nanotechnology, 2011,2,5503-5508] shown good effect.In addition, quantum dot is at CN -[A.Touceda-Varela, E.I.Stevenson, J.A.Galve-Gasi ó n, D.T.F.Dryden and J.C.Mareque-Rivas.Chem.Commun., 2008,1998-2000; W.J.Jin, M.T.Fern á ndez-Arg ü elles, J.M.Costa-Fern á ndez, R.Pereiro and A.Sanz-Medel.Chem.Commun., 2005,883-885; ], Cl -[M.J.Ruedas-Rama and E.A.H.Hall.Analyst, 2008,133,1556-1566], I -Anionic mensuration aspects such as [H.Li, C.Han and L.Zhang.J.Mater.Chem., 2008,18,4543-4548] has also shown stronger superiority.Above-mentioned research is based on all that the quenching effect of ion pair quantum dot detects.But several factors can cause the fluorescent quenching of quantum dot in the solution, can cause spurious signal.The present invention utilizes " co " type principle to set up the novel assay method of phosphate anion, has eliminated in the solution other factors to the undesired signal of quantum dot quencher, successfully carried out phosphate anion fast, efficient measurement.As far as we know, this method be quantum dot as fluorescence probe first in the application aspect the phosphate anion mensuration.
Summary of the invention:
The assay method that the purpose of this invention is to provide a kind of phosphate anion fast and efficiently; Especially provide quantum dot as the nanocomposite optical probe in the new purposes aspect the negative ion mensuration.
One of the object of the invention can be realized through following technical measures:
A, a certain amount of coating material and after the cadmium salt soln of 80ml 0.001M mixes add the pH of the NaOH solution regulator solution of 0.1M;
In b, the above-mentioned mixed liquor, feed high pure nitrogen after 30 minutes, add the Na of 20ml 0.002M 2S (perhaps NaHSe, the NaHTe) WS, the logical nitrogen of continuation stirs reaction down 20 minutes, gets water-soluble CdX (X represents sulphur, selenium, tellurium) nano material;
C, water-soluble CdX (X represents sulphur, selenium, the tellurium) nano material of 0.25ml gained is mixed with the Tris-HCl buffer solution of 0.65ml 0.1M; Add certain density rare-earth ion solution; React after 1 minute, the simulated water sample that adds the phosphate anion standard solution to be measured of variable concentrations or contain phosphate anion is carried out fluorometric assay.
The object of the invention also can be realized through following technical measures:
The coating material of described CdX (X represents sulphur, selenium, tellurium) nano material, difference selected from mercapto acetate, mercaptopropionic acid, halfcystine, trisodium citrate, sodium tartrate; The amount of described coating material be cadmium ion amount of substance 1-5 doubly; Described cadmium salt soln is selected from a kind of in cadmium nitrate, cadmium sulfate, caddy, the cadmium perchlorate; The pH value of solution of regulating during described synthetic CdX (X represents sulphur, selenium, tellurium) nano material is 7.0-11.0; Described rare earth ion is respectively a kind of in lanthanum, cerium, praseodymium, neodymium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium, the yttrium; The concentration of described rare earth ion is 1 * 10 -6-1 * 10 -5Mol/L.
Quantum dot probe fluorescence generation quencher in the presence of rare earth ion that the present invention is prepared, phosphate anion add back fluorescence and obviously recover.This " co " type probe can effectively be avoided the interference of the fluorescent quenching that other factors causes in the solution, and selectivity improves greatly.
Description of drawings:
Fig. 1 invents the CdS quantum dot (a) of the cysteine modified for preparing and adds 1.0 * 10 -6The cerium ion of mol/L and 6.0 * 10 -6The fluorescence spectrum of the phosphate anion of mol/L.
Fig. 2 is the graph of a relation that the fluorescence intensity of the CdS quantum dot modified of the TGA of invention preparation changes with phosphorus acid ion concentration.
Fig. 3 invents the CdS quantum dot of the TGA modification for preparing to other anionic fluorescence response figure.
Fig. 4 is effect is measured in the variation of pH value of solution to phosphate anion influence.
Fig. 5 is Ce 3+Change in concentration phosphate anion is measured the influence of effect.
Embodiment:
Embodiment 1:
The CdCl of the cysteine hydrochloride of a, 0.013g and 80ml 0.001M 2After solution mixed, the pH that adds the NaOH solution regulator solution of 0.1M was 7.5;
In b, the above-mentioned mixed liquor, feed high pure nitrogen after 30 minutes, add the Na of 20ml 0.002M 2The S WS, the logical nitrogen of continuation stirs reaction down 20 minutes, gets water-soluble CdS nano material;
C, the water-soluble CdS nano material of 0.25ml gained is mixed with the Tris-HCl buffer solution of 0.65ml 0.1M, add 1.0 * 10 of 0.5ml -4The lanthanum nitrate hexahydrate of mol/L reacted after 1 minute, and the simulated water sample that adds the phosphate anion standard solution to be measured of variable concentrations or contain phosphate anion is carried out fluorometric assay.
Embodiment 2:
The TGA solution of a, 10mL 0.01mol/L and the Cd (ClO of 80ml 0.001M 4) 2After solution mixed, the pH that adds the NaOH solution regulator solution of 0.1M was 9.0;
In b, the above-mentioned mixed liquor, feed high pure nitrogen after 30 minutes, add the NaHSe WS of 20ml 0.002M, the logical nitrogen of continuation stirs reaction down 20 minutes, gets the water-soluble CdSe nano material;
C, the water-soluble CdSe nano material of 0.25ml gained is mixed with the Tris-HCl buffer solution of 0.65ml 0.1M, add 1.0 * 10 of 0.1ml -4The cerous sulfate of mol/L (III) solution reacted after 1 minute, and the phosphate anion standard solution to be measured that adds variable concentrations carries out fluorometric assay.
Embodiment 3:
The two hydration trisodium citrates of a, 0.118g and the Cd (ClO of 80ml 0.001M 4) 2After solution mixed, the pH that adds the NaOH solution regulator solution of 0.1M was 7.0;
In b, the above-mentioned mixed liquor, feed high pure nitrogen after 30 minutes, add the NaHTe WS of 20ml 0.002M, the logical nitrogen of continuation stirs reaction down 20 minutes, gets the water-soluble CdTe nano material;
C, the water-soluble CdTe nano material of 0.25ml gained is mixed with the Tris-HCl buffer solution of 0.65ml 0.1M, add 1.0 * 10 of 0.1ml -4The europium nitrate solution of mol/L (is used Eu 2O 3Adding WS heating behind the nitric acid dissolve will evaporate nitric acid and make), react after 1 minute, adding contains 1.0 * 10 -5The simulated water sample 1mL of mol/L phosphate anion carries out fluorometric assay.
Embodiment 4:
Cd (the NO of the disodium tartrate of a, 0.100g and 80ml 0.001M 3) 2After solution mixed, the pH that adds the NaOH solution regulator solution of 0.1M was 11.0;
In b, the above-mentioned mixed liquor, feed high pure nitrogen after 30 minutes, add the NaHTe WS of 20ml 0.002M, the logical nitrogen of continuation stirs reaction down 20 minutes, gets the water-soluble CdTe nano material;
C, the water-soluble CdTe nano material of 0.25ml gained is mixed with the Tris-HCl buffer solution of 0.65ml 0.1M, add 1.0 * 10 of 0.5ml -4The europium nitrate solution of mol/L (is used Eu 2O 3Adding WS heating behind the nitric acid dissolve will evaporate nitric acid and make), react after 1 minute, adding contains 1.0 * 10 -5The simulated water sample 1mL of mol/L phosphate anion carries out fluorometric assay.

Claims (6)

  1. Phosphate anion fast, the high-efficiency fluorescence detection method, it is characterized in that:
    A, a certain amount of coating material and after the cadmium salt soln of 80ml 0.001M mixes add the pH of the NaOH solution regulator solution of 0.1M;
    In b, the above-mentioned mixed liquor, feed high pure nitrogen after 30 minutes, add the Na of 20ml 0.002M 2S (perhaps NaHSe, the NaHTe) WS, the logical nitrogen of continuation stirs reaction down 20 minutes, gets water-soluble CdX (X represents sulphur, selenium, tellurium) nano material;
    C, water-soluble CdX (X represents sulphur, selenium, the tellurium) nano material of 0.25ml gained is mixed with the Tris-HCl buffer solution of 0.65ml 0.1M; Add certain density rare-earth ion solution; React after 1 minute, the simulated water sample that adds the phosphate anion standard solution to be measured of variable concentrations or contain phosphate anion is carried out fluorometric assay.
  2. Phosphate anion according to claim 1 fast, efficient detection method, it is characterized in that the coating material of described CdS nano material being selected from high TGA respectively, mercaptopropionic acid, halfcystine, trisodium citrate, sodium tartrate; The amount of described coating material be cadmium ion amount of substance 1-5 doubly.
  3. Phosphate anion according to claim 1 fast, efficient detection method, it is characterized in that described cadmium salt soln is selected from a kind of in cadmium nitrate, cadmium sulfate, caddy, the cadmium perchlorate.
  4. Phosphate anion according to claim 1 fast, efficient detection method, it is characterized in that the pH value of solution of regulating when synthetic is 7.0-11.0.
  5. Phosphate anion according to claim 1 fast, efficient detection method, it is characterized in that the rare earth ion of selecting for use is respectively a kind of in lanthanum, cerium, praseodymium, neodymium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium, the yttrium.
  6. Phosphate anion according to claim 1 fast, efficient detection method, the concentration of the rare earth ion that it is characterized in that selecting for use is 1 * 10 -6-1 * 10 -5Mol/L.
CN2011103993309A 2011-12-06 2011-12-06 Quick and high-efficiency fluorescence detection method for phosphate ions Pending CN102495035A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2011103993309A CN102495035A (en) 2011-12-06 2011-12-06 Quick and high-efficiency fluorescence detection method for phosphate ions

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2011103993309A CN102495035A (en) 2011-12-06 2011-12-06 Quick and high-efficiency fluorescence detection method for phosphate ions

Publications (1)

Publication Number Publication Date
CN102495035A true CN102495035A (en) 2012-06-13

Family

ID=46186876

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2011103993309A Pending CN102495035A (en) 2011-12-06 2011-12-06 Quick and high-efficiency fluorescence detection method for phosphate ions

Country Status (1)

Country Link
CN (1) CN102495035A (en)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104017582A (en) * 2014-06-12 2014-09-03 安徽师范大学 Fluorescent probe and detection method of citrate in human urine
CN104267029A (en) * 2014-10-23 2015-01-07 攀枝花钢企欣宇化工有限公司 Quantitative analysis method for phosphate radical
CN106349167A (en) * 2016-10-21 2017-01-25 齐齐哈尔大学 Benzimidazole derivative phosphate anion fluorescence probe synthesis and application method
CN106525806A (en) * 2017-01-13 2017-03-22 中国农业大学 Method for detecting ethrel by using fluorescent nitrogen doped carbon quantum dot
CN107356577A (en) * 2017-07-25 2017-11-17 陕西师范大学 A kind of universal sulfotransferase activity assays
CN110095434A (en) * 2019-06-17 2019-08-06 广西师范大学 A method of phosphate radical is measured with Resonance Rayleigh Scattering Spectra
CN111257291A (en) * 2020-01-20 2020-06-09 武汉理工大学 Quantitative detection method for phosphate ions and application thereof
CN111351924A (en) * 2018-12-20 2020-06-30 中国科学院福建物质结构研究所 Near-infrared fluorescence immunoassay kit based on enzyme-induced phosphate ion activation and detection method
CN111521594A (en) * 2020-05-13 2020-08-11 重庆工程职业技术学院 Novel nano-composite, preparation method thereof and application thereof in sewage detection
CN112280556A (en) * 2020-11-14 2021-01-29 西北农林科技大学 Preparation of phosphate radical responsive carbon quantum dots and application of phosphate radical responsive carbon quantum dots in fingerprint fluorescence identification
CN114849659A (en) * 2022-05-27 2022-08-05 湖南大学 Preparation method and application of lanthanum-iron-loaded chitosan microsphere adsorbent for removing heavy metal cadmium and phosphate in water

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101973514A (en) * 2010-09-07 2011-02-16 江南大学 Synthesis and application of water soluble ZnxCd(1-x)S nano composite material used for copper ion determination

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101973514A (en) * 2010-09-07 2011-02-16 江南大学 Synthesis and application of water soluble ZnxCd(1-x)S nano composite material used for copper ion determination

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
曹春 等: "基于自然尺寸分布的单一CdTe量子点样品中Eu(III)诱导的能量转移测定磷酸根离子", 《中国科学：化学》 *

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104017582A (en) * 2014-06-12 2014-09-03 安徽师范大学 Fluorescent probe and detection method of citrate in human urine
CN104267029A (en) * 2014-10-23 2015-01-07 攀枝花钢企欣宇化工有限公司 Quantitative analysis method for phosphate radical
CN106349167A (en) * 2016-10-21 2017-01-25 齐齐哈尔大学 Benzimidazole derivative phosphate anion fluorescence probe synthesis and application method
CN106525806A (en) * 2017-01-13 2017-03-22 中国农业大学 Method for detecting ethrel by using fluorescent nitrogen doped carbon quantum dot
CN106525806B (en) * 2017-01-13 2019-05-21 中国农业大学 A method of utilizing fluorescence nitrogen-doped carbon quantum dots characterization ethephon (CEPHA),2-(chloroethyl) phosphonic acid
CN107356577A (en) * 2017-07-25 2017-11-17 陕西师范大学 A kind of universal sulfotransferase activity assays
CN111351924A (en) * 2018-12-20 2020-06-30 中国科学院福建物质结构研究所 Near-infrared fluorescence immunoassay kit based on enzyme-induced phosphate ion activation and detection method
CN110095434A (en) * 2019-06-17 2019-08-06 广西师范大学 A method of phosphate radical is measured with Resonance Rayleigh Scattering Spectra
CN111257291A (en) * 2020-01-20 2020-06-09 武汉理工大学 Quantitative detection method for phosphate ions and application thereof
CN111257291B (en) * 2020-01-20 2020-12-22 武汉理工大学 Quantitative detection method for phosphate ions and application thereof
CN111521594A (en) * 2020-05-13 2020-08-11 重庆工程职业技术学院 Novel nano-composite, preparation method thereof and application thereof in sewage detection
CN112280556A (en) * 2020-11-14 2021-01-29 西北农林科技大学 Preparation of phosphate radical responsive carbon quantum dots and application of phosphate radical responsive carbon quantum dots in fingerprint fluorescence identification
CN112280556B (en) * 2020-11-14 2022-12-09 西北农林科技大学 Preparation of phosphate radical responsive carbon quantum dots and application of phosphate radical responsive carbon quantum dots in fingerprint fluorescence identification
CN114849659A (en) * 2022-05-27 2022-08-05 湖南大学 Preparation method and application of lanthanum-iron-loaded chitosan microsphere adsorbent for removing heavy metal cadmium and phosphate in water

Similar Documents

Publication Publication Date Title
CN102495035A (en) Quick and high-efficiency fluorescence detection method for phosphate ions
Che et al. A portable logic detector based on Eu-MOF for multi-target, on-site, visual detection of Eu3+ and fluoride in groundwater
Hu et al. Highly selective fluorescent sensors for Hg2+ based on bovine serum albumin-capped gold nanoclusters
Zhao et al. Highly selective detection of phosphate in very complicated matrixes with an off–on fluorescent probe of europium-adjusted carbon dots
Jiang et al. Phosphorus speciation in sediments of Lake Hongfeng, China
Sánchez-Rodríguez et al. Comparative effects of prolonged freshwater and saline flooding on nitrogen cycling in an agricultural soil
Cesur Determination of manganese, copper, cadmium and lead by FAAS after solid-phase extraction of their phenylpiperazine dithiocarbamate complexes on activated carbon
Qin et al. Water-soluble silica-coated ZnS: Mn nanoparticles as fluorescent sensors for the detection of ultratrace copper (II) ions in seawater
Willey et al. Rainwater as a source of Fe (II)‐stabilizing ligands to seawater
Chaiendoo et al. A highly selective colorimetric sensor for ferrous ion based on polymethylacrylic acid-templated silver nanoclusters
CN102994092A (en) Preparation of cysteine surface modified CdTe or CdTe/CdS quantum dot and method for detecting arsenic by using quantum dot
Jin et al. Preparation of Novel Arrays Silver Nanoparticles Modified Polyrutin Coat‐Paraffin‐Impregnated Graphite Electrode for Tyrosine and Tryptophan's Oxidation
Song et al. Highly sensitive and selective detection of phosphate using novel highly photoluminescent water-soluble Mn-doped ZnTe/ZnSe quantum dots
Chen et al. Conservative behavior of terrestrial trace elements associated with humic substances in the coastal ocean
Carolan et al. Germanium nanocrystals as luminescent probes for rapid, sensitive and label-free detection of Fe 3+ ions
CN112744797A (en) Nitrogen-boron doped graphite phase carbon nitride quantum dot and preparation method and application thereof
Hu et al. Phosphorus recovery and resource utilization from phosphogypsum leachate via membrane-triggered adsorption and struvite crystallization approach
Chen et al. Role of terrestrial versus marine sources of humic dissolved organic matter on the behaviors of trace elements in seawater
Wang et al. Electrodeposition and characterization of CaF2 and rare earth doped CaF2 films
Tan et al. Room temperature phosphorescence sensor for Hg2+ based on Mn-doped ZnS quantum dots
CN103865527B (en) The preparation method of a kind of phosphorescence quantum dot Mn-ZnS and Iron speciation analyze in application
Speidel et al. Rivers and tidal flats as sources of dissolved organic matter and trace metals in the German Bight (North Sea)
XIA et al. Selective Detection of Mercury (II) and Copper (II) Based on the Opposite Size‐dependent Fluorescence Quenching of CdTe Quantum Dots
Ghatak et al. Interactions of Cu (II) and Fe (III) with mangal humic substances studied by synchronous fluorescence spectroscopy and potentiometric titration
Chen et al. An “off-on” fluorescent nanosensor for the detection of cadmium ions based on APDC-etched CdTe/CdS/SiO2 quantum dots

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20120613