CN102488757B - Method for extracting flavone from peony pollen - Google Patents
Method for extracting flavone from peony pollen Download PDFInfo
- Publication number
- CN102488757B CN102488757B CN201110411086.3A CN201110411086A CN102488757B CN 102488757 B CN102488757 B CN 102488757B CN 201110411086 A CN201110411086 A CN 201110411086A CN 102488757 B CN102488757 B CN 102488757B
- Authority
- CN
- China
- Prior art keywords
- extraction
- flavone
- pollen
- peony pollen
- flash
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Fats And Perfumes (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
The invention discloses a method for extracting flavone from peony pollen by using a flash extraction method. The extraction comprises the following steps of: (1) crushing air-dried peony pollen and screening with a sieve of 50-100 meshes; (2) dissolving the processed peony pollen into 30-100 percent ethanol according to material-liquid ratio of 1:10-1:50; (3) performing flash extraction on the solution obtained by the step (2), and centrifugally separating extract, wherein the supernatant is flavone extract; and (3) performing the flash extraction under the conditions that the rotation speed of a flash extractor is from gear one to gear four and the extraction time is 2-10 minutes, and centrifuging the extract for 5-20 minutes at the rate of 3,000-10,000 r/min during flash extraction. Through the flash extraction method for flavone from peony pollen provided by the invention, the product flavone can be obtained at a higher speed on the premise of guaranteeing a certain extraction rate; and the method has the characteristics of high speed, high efficiency and suitability for industrial production.
Description
Technical field
The present invention relates to the method utilizing homogenate extraction method to extract flavone from peony pollen.
Background technology
Monoterpene glycosides, phenols, tannin and some sterols, flavone, acid, ester is rich in, alkane in Radix Paeoniae pollen. volatile oil, aminoacid, the compounds such as protein.Wherein flavone has blood circulation invigorating efficacies and has good effect to treatment coronary heart disease, cerebrovascular disease and thrombotic disease.The domestic and international research of the extraction to flavone in Radix Paeoniae pollen is considerably less at present, and general extracting method extraction efficiency is not high, is not suitable for large production requirement, and complex steps.The extracting method that current homogenate extraction flavone is regarded as the most effective, the most convenient, most environmental protection is tested through multiple extracting method.Its leaching process is combined by breaking cellular wall and extraction, and principle is cutter high speed rotating in high-speed electric expreess locomotive drives, and produces powerful shear action between inside and outside cutter, and outer cutter intracavity produces powerful negative pressure simultaneously, and form vacuum direct current, continuous print carries out fragmentation to tissue.The stirring of this high speed can remove pollen wall effectively, and the flavone in pollen is dissolved in the middle of solvent fully, thus reaches object that is quick, high efficiency extraction pollen flavone.
Summary of the invention
The object of this invention is to provide a kind of method of energy-efficient extraction flavone from peony pollen.
The method of extraction flavone from peony pollen provided by the invention, comprises the steps:
1) the Radix Paeoniae pollen dried in the shade is pulverized, cross bolter;
2) by through 1) the Radix Paeoniae pollen that processes dissolves in ethanol by solid-liquid ratio;
3) by 2) solution carries out homogenate extraction, and extracting solution centrifugalize, supernatant is flavone extractive.
Step 1) in bolter be 50 ~ 100 orders.
Step 2) in solid-liquid ratio be 1: 10 ~ 1: 50; Concentration of alcohol is 30% ~ 100%.
Step 3) when carrying out homogenate extraction, flash extracter rotating speed is 1 ~ 4 grade, extraction time 2 ~ 10min; Extracting solution is with the centrifugal 5 ~ 20min of 3000 ~ 10000r/min.
In addition, the flavone from peony pollen prepared according to the method described above, also belongs to protection scope of the present invention.
Flavone from peony pollen homogenate extraction method provided by the invention has feature fast and efficiently.
Accompanying drawing explanation
The scanning electron microscope (SEM) photograph of Fig. 1 Radix Paeoniae pollen wall after homogenate extraction
The scanning electron microscope (SEM) photograph of Fig. 2 Radix Paeoniae pollen wall after micronizing
Detailed description of the invention
Embodiment 1, homogenate extraction flavone from peony pollen
1) the Radix Paeoniae pollen dried in the shade is pulverized, cross 100 mesh sieves;
2) 10g is taken through 1) the Radix Paeoniae pollen that processes is placed in beaker, adds 100mL ethanol (solid-liquid ratio 1: 10).
3) flash extracter is set, opens switch, cutter head is risen;
4) Radix Paeoniae pollen solution is placed on operating board, falls cutter head; Cutter head falls puts 1cm at the bottom of distance beaker in liquid level, (cutter head can not contact with at the bottom of beaker).
5) gear to be regulated is 4 grades, starts timing 3min.Carry out dodging that should be noted in advance can not regulation stall suddenly, need slow regulation stall after cutter head rotates, be adjusted to the gear of needs again.
6) will through 5) extracting solution that processes with the centrifugal 5min of 10000r/min, get supernatant, calculate flavones content.
Example 2, homogenate extraction flavone from peony pollen and reflow method extract flavone from peony pollen and compare
1) pollen breaking cellular wall all in two photos can be found by Fig. 1 and Fig. 2, but breaking cellular wall degree has little difference.The degree of pollen broken wall shown in Fig. 2 is slightly better than Fig. 1, illustrate that the shell-broken effect of micronizing to pollen is slightly better than homogenate extraction, but micronizing needs 30min to pulverize raw material, and the effect that homogenate extraction only needs 3min can reach substantially identical.
2) homogenate extraction flavone from peony pollen and conventional backflow extract flavone from peony pollen and compare
Table 1 homogenate extraction and conventional backflow extract condition and the result of flavone from peony pollen
Substantially identical by the extraction ratio of the visible two kinds of extracting method of table 1, but homogenate extraction breaking cellular wall and extract be carry out simultaneously and only need 3min just can reach circumfluence method extraction level; And reflux extraction method extraction time is 90min and need to carry out broken wall treatment (micronizing) to pollen.So homogenate extraction shows fast compared with conventional backflow method, efficient advantage.
Claims (3)
1. extract a method for flavone from peony pollen, comprise the steps:
1) the Radix Paeoniae pollen dried in the shade is pulverized, cross bolter;
2) by through 1) the Radix Paeoniae pollen that processes dissolves in certain proportion ethanol by certain solid-liquid ratio; Described solid-liquid ratio is 1:10; The concentration of described ethanol is 100%;
3) by 2) solution carries out homogenate extraction, extracting solution centrifugalize, and supernatant is flavone extractive; During described homogenate extraction, flash extracter rotating speed is 4 grades, extraction time 3min; Extracting solution is with the centrifugal 5min of 10000r/min.
2. method according to claim 1, is characterized in that: described step 1) in bolter be 50-100 order.
3. the flavone from peony pollen that obtains of method described in claim 1 or 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110411086.3A CN102488757B (en) | 2011-12-12 | 2011-12-12 | Method for extracting flavone from peony pollen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110411086.3A CN102488757B (en) | 2011-12-12 | 2011-12-12 | Method for extracting flavone from peony pollen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102488757A CN102488757A (en) | 2012-06-13 |
| CN102488757B true CN102488757B (en) | 2015-07-22 |
Family
ID=46180658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110411086.3A Active CN102488757B (en) | 2011-12-12 | 2011-12-12 | Method for extracting flavone from peony pollen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102488757B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105748618A (en) * | 2016-03-25 | 2016-07-13 | 青海师范大学 | Technology for extracting total flavonoids of pollen of radix paeoniae rubra with flash extraction method |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106074665B (en) * | 2016-08-08 | 2019-08-20 | 齐鲁工业大学 | Extracting flavonoids method in a kind of mao of Lai leaf |
-
2011
- 2011-12-12 CN CN201110411086.3A patent/CN102488757B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| 植物组织破碎提取法及闪式提取器的创制与实践;刘延泽;《中国天然药物》;20071130;第5卷(第6期);401-407 * |
| 油菜花粉黄酮的提取与纯化;王芙蓉, 梁运祥;《湖北农业科学》;20070331;第46卷(第2期);290-292 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105748618A (en) * | 2016-03-25 | 2016-07-13 | 青海师范大学 | Technology for extracting total flavonoids of pollen of radix paeoniae rubra with flash extraction method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102488757A (en) | 2012-06-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102824377B (en) | Method for extracting functional ingredients from lucid ganoderma sporocarp | |
| CN102517144A (en) | Method for extracting oil and procyanidin from grape seeds | |
| CN102586023A (en) | Method for extracting citrus pericarp essential oil | |
| CN102178346A (en) | Production method for extracting absolute oil of tobacco leaves | |
| CN103555412A (en) | Method for extracting tea-seed oil | |
| CN104312627B (en) | A kind of crystallization process removes solvent and the method for resin in montanin wax | |
| CN103361177B (en) | Method for extraction of aroma compounds from aroma materials | |
| CN103849470A (en) | Preparation method of lacquer tree seed wax oil | |
| CN101979618A (en) | Process for purifying phloretin | |
| CN101993775B (en) | Method for preparing oil by whole peanut kernels | |
| CN102040579A (en) | Method for extracting luteolin from peanut roots, stems, leaves and shells | |
| CN102488757B (en) | Method for extracting flavone from peony pollen | |
| CN103242676A (en) | Method for extracting pericarp pigments of dragon fruit | |
| CN102613639B (en) | Apple processing method based on separation before squeezing | |
| WO2011134411A1 (en) | Method of preparing soybean protein concentrate | |
| CN103463160A (en) | Preparation method of high-content total flavones of chrysanthemum | |
| CN101967177A (en) | Extract and method for extracting tea saponin from oil-tea seed residue | |
| CN104825523A (en) | Wild chrysanthemum flower general flavone extraction method | |
| CN103642594A (en) | Method for extraction of garlic oil | |
| CN103333054A (en) | Method for extracting resveratrol from grape peel residue with enzymatic method | |
| CN108325581B (en) | Comprehensive utilization process of flaxseeds | |
| CN105541957A (en) | Extraction method for increasing content of glycyrrhizic acid in leftover materials of liquorice | |
| CN1266160C (en) | Process for extracting rubusoside of fruit of Gorsvenor Momordica | |
| CN105272989A (en) | Method for preparing chlorophyll sodium copper from mulberry leaves | |
| CN104017099A (en) | Microwave extraction, separation and purification method of pea polysaccharide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20181220 Address after: 215127 No. 8 Gepu Road, Suzhou Industrial Park, Suzhou City, Jiangsu Province Patentee after: Huida Biomedicine (Suzhou) Co., Ltd. Address before: 100048 School of Science, Beijing Business University, 33 Fucheng Road, Beijing Patentee before: Beijing Technology and Business University |