CN102485758A - Glutathione molecular imprinting polymer, preparation method and application thereof - Google Patents
Glutathione molecular imprinting polymer, preparation method and application thereof Download PDFInfo
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- CN102485758A CN102485758A CN2010105723649A CN201010572364A CN102485758A CN 102485758 A CN102485758 A CN 102485758A CN 2010105723649 A CN2010105723649 A CN 2010105723649A CN 201010572364 A CN201010572364 A CN 201010572364A CN 102485758 A CN102485758 A CN 102485758A
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Abstract
The invention relates to a preparation method of a glutathione molecular imprinting polymer. The glutathione molecular imprinting polymer is prepared by using glutathione as a template molecule, carrying out polymerization on functional monomers in a protective gas with existence of cross-linking agent and initiator and removing glutathione in the polymer. Preferably, a specific preparation process comprises steps of: dissolving glutathione in a solvent; adding functional monomers to form a template molecule-functional monomer copolymer; adding the cross-linking agent and the initiator; carrying out polymerization protective gas to obtain the polymer; and washing out glutathione in the polymer. The invention also provides the glutathione molecular imprinting polymer prepared by the method and a method for separating glutathione. The glutathione molecular imprinting polymer of the invention can specifically and selectively separate glutathione, enrich glutathione and provide basis for subsequent application. The preparation method is simple and convenient and suitable for large-scale popularization and application.
Description
Technical field
The present invention relates to the molecularly imprinted polymer technical field, particularly gsh molecularly imprinted polymer technical field specifically is a kind of gsh molecularly imprinted polymer and methods for making and using same thereof, is specially adapted to the separation and purification gsh.
Background technology
Gsh (GSH) is a kind of natural radioactivity tripeptides (L-gamma-glutamyl-L-cysteinyl-glycocoll) with important physiological function; It is biological intravital main inhibitor; Participate in multiple metabolic process in vivo, can protect the organism inner cell to avoid the injury of oxidation and toxic substance; In fields such as clinical medicine, food service industry, cosmetic industries purposes is widely arranged.Recent research finds that GSH also has the effect that suppresses AIDS.
The method of separation and purification gsh is an ion exchange method at present, but IX is relatively more difficult when resin regeneration, and needs a large amount of bronsted lowry acids and bases bronsted lowries; Also have isoelectric point precipitation, but isoelectric point precipitation separation and purification gsh needs then the amount of gsh to reach certain degree, separate out otherwise gsh is difficult to deposition; And in the separation and purification process, pigment adopts multiple organic solvent to extract all to be difficult to remove, all the time in whole purge process.
Molecularly imprinted polymer is the mechanism according to Ag-Ab, selects to have with template molecule the function monomer of interaction force, and corresponding crosslink agent, initiator and solvent.Through polyreaction, function monomer forms certain reticulated structure, through the method for physics or chemistry template molecule is removed then, forming specific space binding site, thereby template molecule is had special evident characteristics ability.
Therefore, expectation provides a kind of gsh molecularly imprinted polymer, thereby can separate gsh by specific selectivity, and the enrichment gsh is for subsequent applications provides the basis.
Summary of the invention
The objective of the invention is to have overcome above-mentioned shortcoming of the prior art; A kind of gsh molecularly imprinted polymer and methods for making and using same thereof are provided; This gsh molecularly imprinted polymer can separate gsh by specific selectivity, and the enrichment gsh is for subsequent applications provides the basis; Its preparation method is simple and convenient, is suitable for large-scale promotion application.
To achieve these goals; In first aspect of the present invention; A kind of preparation method of gsh molecularly imprinted polymer being provided, being characterized in, is template molecule with the gsh; Employing is used for the function monomer of gsh, thereby the said gsh that in the presence of linking agent and initiator, in shielding gas, carries out removing in the said polymkeric substance after polyreaction obtains polymkeric substance obtains said gsh molecularly imprinted polymer.
Preferably; Its concrete preparation process is: said gsh is dissolved in the solvent; Add said function monomer then and form template molecule-function monomer multipolymer; Add said linking agent and said initiator again, in said shielding gas, carry out said polyreaction and obtain the said gsh that wash-out is removed in the said polymkeric substance behind the said polymkeric substance and obtain said gsh molecularly imprinted polymer.
Those skilled in the art know; The scope that function monomer and linking agent are selected is bigger; Function monomer for example can select methylacrylic acid, acrylic amide, to Ethenylbenzene formic acid, TEB 3K, methylene-succinic acid etc., linking agent can be selected for example ethylene glycol dimethacrylate, methylene diacrylamide, pentaerythritol triacrylate etc.More preferably, said solvent is a methyl-sulphoxide, and said function monomer is methylacrylic acid or acrylic amide, and said linking agent is an ethylene glycol dimethacrylate, and said initiator is a Diisopropyl azodicarboxylate, and said shielding gas is a nitrogen.Various materials add the ultrasonic exhaust 10～15min in back; Behind the ultrasonic mixing, feed nitrogen 10～15min, and in nitrogen state lower seal reaction system.
More preferably, said gsh passes through ultrasonic mixing in said solvent, the dissolving of spending the night, and concentration is 0.067～0.1mol/l.
More preferably, the molar ratio of said template molecule and said function monomer is 1: 2～1: 10, and the molar ratio of said function monomer and said linking agent is 1: 4.5～5, and the quality of said initiator is about 1% of said function monomer quality.
More preferably, said polyreaction was carried out 20-24 hour at 60-65 ℃.Promptly adopt the mode of thermal initiation to carry out polyreaction.
Polyreaction is pulverized polymkeric substance, grind, sieve after accomplishing, and adopts the elutriant wash-out to remove template molecule then, and is till detecting less than template molecule, dry then.More preferably, adopting concentration is that the sodium chloride solution wash-out of 0.6-0.8mol/l is removed the said gsh in the said polymkeric substance.
In second aspect of the present invention, a kind of gsh molecularly imprinted polymer is provided, be characterized in, adopt the preparation method of above-mentioned gsh molecularly imprinted polymer to be prepared from.
In the third aspect of the invention; A kind of method that above-mentioned gsh molecularly imprinted polymer is applied to separate gsh is provided; Be characterized in; The solution stream that will contain gsh is carried out the special absorption of selectivity through described gsh molecularly imprinted polymer, thus the said gsh of purifying.
Preferably, the described concentration that contains the solution of gsh is 1.5-2.0g/l, and pH is 4.8-5.3, and the Static Adsorption starting time of the special absorption of said selectivity is 18-19h.
Beneficial effect of the present invention is: gsh molecularly imprinted polymer of the present invention is to be template molecule with the gsh; Employing is used for the function monomer of gsh, thereby the said gsh that in the presence of linking agent and initiator, in shielding gas, carries out removing in the said polymkeric substance after polyreaction obtains polymkeric substance obtains, and can separate gsh by specific selectivity; The enrichment gsh; For subsequent applications provides the basis, its preparation method is simple and convenient, is suitable for large-scale promotion application.
Description of drawings
Fig. 1 is the gsh molecularly imprinted polymer figure that the preparing method's of a gsh molecularly imprinted polymer of the present invention specific embodiment makes.
Fig. 2 is the gsh molecularly imprinted polymer figure that another specific embodiment of the preparation method of gsh molecularly imprinted polymer of the present invention makes.
Fig. 3 be preparing method's the mol ratio of template molecule and function monomer of gsh molecularly imprinted polymer of the present invention to the synoptic diagram that influences of polymkeric substance absorption property, wherein MIP1, MIP2, MIP3, MIP4, MIP5 respectively the mol ratio of representation template molecule and function monomer be 1: 2; 1: 4; 1: 5; 1: 8; The gsh molecularly imprinted polymer of processing at 1: 10.
Fig. 4 is the influence synoptic diagram of last appearance pH to gsh molecularly imprinted polymer absorption property of the present invention.
Fig. 5 is the influence synoptic diagram of last appearance concentration to gsh molecularly imprinted polymer absorption property of the present invention.
Fig. 6 is the influence synoptic diagram of adsorption time to gsh molecularly imprinted polymer absorption property of the present invention.
Embodiment
In order more to be expressly understood technology contents of the present invention, the special following examples of lifting specify, and its purpose only is better to understand content of the present invention and unrestricted protection scope of the present invention.
Embodiment 1Gsh molecularly imprinted polymer (function monomer is a methylacrylic acid) synthesizes instance
1.0mmol template molecule gsh (GSH) is dissolved in the solvent methyl-sulphoxide of 15ml; Ultrasonic mixing, and the dissolving of spending the night carry out packing with lysate then; Every part of 3ml (containing template molecule 0.2mmol) is according to template molecule: function monomer=1: 2; 1: 4; 1: 5; 1: 8; 1: 10 mol ratio adds respective amount (0.4; 0.8; 1.0; 1.6; 2.0mmol) function monomer methylacrylic acid MAA; Again according to function monomer: linking agent=1: 5 (2.0; 4.0; 5.0; 8.0; 10.0mmol) molar ratio add the linking agent ethylene glycol dimethacrylate EGDMA of respective amount and initiator Diisopropyl azodicarboxylate AIBN.Ultrasonic degas 10min behind the ultrasonic mixing, feeds nitrogen 10min, in the state lower seal reaction system of nitrogen.60 ℃ of reaction 24h; Polyreaction is pulverized the synthetic polymkeric substance, grind, sieve after finishing, and removes template molecule with sodium chloride solution then, till detecting less than template molecule; The polymkeric substance of removing template molecule is carried out drying, obtain the molecularly imprinted polymer that size is 50-75 μ m at last, like Fig. 1, shown in 2.
(1) optimum mole ratio (template molecule-function monomer) confirms
Respectively getting 5ml concentration is the GSH sample solution of 1.6g/l, adds the molecularly imprinted polymer (GSH: MAA=1: 2 of the above-mentioned employing different mol ratio preparation of 100mg respectively; 1: 4; 1: 5; 1: 8; 1: 0), vibration absorption certain hour, carry out centrifugal (5000rpm, 10min); The concentration of GSH in the supernatant under the mensuration different condition, Units of Account adsorptive capacity, and specific factor; See also shown in Figure 3ly, experimental result shows, along with the increase of mol ratio; Molecularly imprinted polymer reduces first the increasing afterwards of the unit adsorptive capacity of GSH, wherein with GSH: MAA=1: the 4th, maximum, be 12632 μ g/g.When mol ratio is low, can't form complete recognition site, can not realize that then function monomer combines with the multidigit point of template molecule; When mol ratio was higher, then function monomer was superfluous, can cause non-specific adsorption to strengthen; Therefore select GSH: MAA=1: be preferable synthetic mol ratio at 2～1: 10, selects GSH: MAA=1: 4 is best synthetic mol ratio.
(2) the best pH's of GSH sample solution confirms
The concentration of respectively getting 5ml is the GSH sample solution of 1.6g/l, and using hydrochloric acid to be adjusted to pH respectively is 1.0; 2.0; 3.5; 5.0; 7.0; 9.0; 11.0; The molecularly imprinted polymer MIP2 that adds 100mg, vibration absorption certain hour carries out centrifugal (5000rpm; 10min), the concentration of GSH in the supernatant under the mensuration different condition, Units of Account adsorptive capacity; See also shown in Figure 4ly, experimental result shows, along with the rising of pH; Molecularly imprinted polymer reduces first the increasing afterwards of the unit adsorptive capacity of GSH, and is wherein maximum with the pH5.0 place.Therefore selecting pH is that 4.8-5.3 is the preferable appearance pH that goes up, and selecting pH5.0 is the best appearance pH that goes up.
(3) GSH sample solution optimum concn confirms
Respectively get 100mg molecularly imprinted polymer MIP2, add the GSH sample solution of different concns respectively, concentration is respectively 0.06g/l; 0.25g/l; 0.5g/l; 1.0g/l; 1.5g/l 2.0g/l; 2.5g/l; 3.0g/l, mixing, vibration absorption certain hour, it is centrifugal that (5000rpm, 10min), the concentration of GSH in the supernatant is calculated the unit adsorptive capacity of molecularly imprinted polymer to GSH under the mensuration different condition.See also shown in Figure 5; Experimental result shows that in certain concentration range, molecularly imprinted polymer increases along with the rising of sample solution concentration the unit adsorptive capacity of GSH; Wherein when sample solution concentration is 2g/l; Molecularly imprinted polymer is tending towards adsorption equilibrium to the unit adsorptive capacity of GSH, and blank polymkeric substance then adsorbs and is tending towards saturated, and the difference of both unit adsorptive capacitys increases along with the increase of sample solution concentration; Therefore selecting 1.5-2.0g/l is the preferable appearance concentration that goes up, and selecting 2g/l is the best appearance concentration that goes up.
(4) the adsorption equilibrium time confirms
Respectively get 100mg molecularly imprinted polymer MIP2, add the GSH sample solution 5ml of 1.6g/l respectively, behind the mixing, vibration absorption was measured the concentration of GSH in the primary supernatant in per 2 hours, and molecularly imprinted polymer is to the unit adsorptive capacity of GSH under the calculating different time.See also shown in Figure 6; Experimental result shows that in certain adsorption time, molecularly imprinted polymer increases along with the increase of adsorption time the unit adsorptive capacity of GSH; Wherein when adsorption time was 18h, molecularly imprinted polymer was tending towards adsorption equilibrium to the unit adsorptive capacity of GSH.Template molecule gets in the molecularly imprinted polymer then needs the regular hour, if the time is too short, then molecularly imprinted polymer can not adsorb enough gsh; If adsorption time is oversize, it is free then can to lower efficiency even have the desorb of part gsh molecule; Therefore selecting 18-19h is preferable adsorption time, and selecting adsorption time is that 18h is the optimal adsorption time.
Comparative Examples 1: the preparation of blank polymkeric substance and application thereof
Get 5 parts of dimethylsulfoxide solvent, every part of 3ml is according to template molecule: function monomer=0: 2; 0: 4; 0: 5; 0: 8; 0: 10 mol ratio adds 0.4; 0.8; 1.0; 1.6; 2.0mmol function monomer TEB 3K MMA; According to function monomer: linking agent=1: 5 (2.0; 4.0; 5.0; 8.0; 10.0mmol) molar ratio add the linking agent ethylene glycol dimethacrylate EGDMA of respective amount and initiator Diisopropyl azodicarboxylate AIBN.Ultrasonic degas 10min behind the ultrasonic mixing, feeds nitrogen 10min, in the state lower seal reaction system of nitrogen.60 ℃ of reaction 24h; Polyreaction is pulverized the synthetic polymkeric substance, grind, sieve after finishing, and removes template molecule with sodium chloride solution then, till detecting less than template molecule; The polymkeric substance of removing template molecule is carried out drying, obtain the molecularly imprinted polymer that size is 50-75 μ m at last.
Adopt blank polymkeric substance to carry out best sample solution pH, best sample solution concentration, the adsorption equilibrium time confirm that experimentation is with corresponding experimentation among the embodiment 1, the result shows that blank polymkeric substance does not have adsorptive power basically.
Embodiment 2: the synthetic instance of gsh molecularly imprinted polymer (function monomer is an acrylic amide)
1.0mmol template molecule gsh (GSH) is dissolved in the solvent methyl-sulphoxide of 15ml; Ultrasonic mixing, and the dissolving of spending the night carry out packing with lysate then; Every part of 3ml (containing template molecule 0.2mmol) is according to template molecule: function monomer=1: 2; 1: 4; 1: 5; 1: 8; 1: 10 mol ratio adds respective amount (0.4; 0.8; 1.0; 1.6; 2.0mmol) function monomer acrylic amide AM; Again according to function monomer: linking agent=1: 5 (2.0; 4.0; 5.0; 8.0; 10.0mmol) molar ratio add the linking agent ethylene glycol dimethacrylate EGDMA of respective amount and initiator Diisopropyl azodicarboxylate AIBN.Ultrasonic degas 10min behind the ultrasonic mixing, feeds nitrogen 10min, in the state lower seal reaction system of nitrogen.60 ℃ of reaction 24h; Polyreaction is pulverized the synthetic polymkeric substance, grind, sieve after finishing, and removes template molecule with sodium chloride solution then, till detecting less than template molecule; The polymkeric substance of removing template molecule is carried out drying, obtain the molecularly imprinted polymer that size is 50-75 μ m at last, like Fig. 1, shown in 2.
(1) optimum mole ratio (template molecule-function monomer) confirms
Respectively getting 5ml concentration is the GSH sample solution of 1.6g/l, adds the molecularly imprinted polymer (GSH: AM=1: 2 of the above-mentioned employing different mol ratio preparation of 100mg respectively; 1: 4; 1: 5; 1: 8; 1: 0), vibration absorption certain hour carries out centrifugal (5000rpm; 10min), the concentration of GSH in the supernatant under the mensuration different condition, Units of Account adsorptive capacity; And specific factor, see also shown in Figure 3ly, experimental result shows; Along with the increase of mol ratio, molecularly imprinted polymer reduces first the increasing afterwards of the unit adsorptive capacity of GSH, wherein with GSH: AM=1: the 4th, maximum.When mol ratio is low, can't form complete recognition site, can not realize that then function monomer combines with the multidigit point of template molecule; When mol ratio was higher, then function monomer was superfluous, can cause non-specific adsorption to strengthen, and is similar with the polymer property that adopts the preparation of function monomer methylacrylic acid; Therefore select GSH: MAA=1: be preferable synthetic mol ratio at 2～1: 10, selects GSH: AM=1: 4 is best synthetic mol ratio.
(2) the best pH's of GSH sample solution confirms
The concentration of respectively getting 5ml is the sample solution of 1.6g/l, and using hydrochloric acid to be adjusted to pH respectively is 1.0; 2.0; 3.5; 5.0; 7.0; 9.0; 11.0; The molecularly imprinted polymer MIP2 that adds 100mg, vibration absorption certain hour carries out centrifugal (5000rpm; 10min), the concentration of GSH in the supernatant under the mensuration different condition, Units of Account adsorptive capacity; See also shown in Figure 4ly, experimental result shows, along with the rising of pH; Molecularly imprinted polymer reduces first the increasing afterwards of the unit adsorptive capacity of GSH, and is wherein maximum about the pH5.0 place, similar with the polymer property that adopts the preparation of function monomer methylacrylic acid; Therefore selecting pH is that 4.8-5.3 is the preferable appearance pH that goes up, and selecting pH5.0 is the best appearance pH. that goes up
(3) the sample solution optimum concn confirms
Respectively get 100mg molecularly imprinted polymer MIP2, add the GSH sample solution of different concns respectively, concentration is respectively 0.06g/l; 0.25g/l; 0.5g/l; 1.0g/l; 1.5g/l 2.0g/l; 2.5g/l; 3.0g/l, mixing, vibration absorption certain hour, it is centrifugal that (5000rpm, 10min), the concentration of GSH in the supernatant is calculated the unit adsorptive capacity of molecularly imprinted polymer to GSH under the mensuration different condition.See also shown in Figure 5ly, experimental result shows, in certain concentration range; Molecularly imprinted polymer increases along with the rising of sample solution concentration the unit adsorptive capacity of GSH; Wherein when sample solution concentration was about 2g/l, molecularly imprinted polymer was tending towards adsorption equilibrium to the unit adsorptive capacity of GSH, and blank polymkeric substance then adsorbs and is tending towards saturated; The difference of both unit adsorptive capacitys increases along with the increase of sample solution concentration, and is similar with the polymer property that adopts the preparation of function monomer methylacrylic acid; Therefore selecting 1.5-2.0g/l is the preferable appearance concentration that goes up, and selecting 2g/l is the best appearance concentration that goes up.
(4) the adsorption equilibrium time confirms
Respectively get 100mg molecularly imprinted polymer MIP2, add the sample solution 5ml of 1.6g/l respectively, behind the mixing, vibration absorption was measured the concentration of GSH in the primary supernatant in per 2 hours, and molecularly imprinted polymer is to the unit adsorptive capacity of GSH under the calculating different time.See also shown in Figure 6; Experimental result shows; In certain adsorption time, molecularly imprinted polymer increases along with the increase of adsorption time the unit adsorptive capacity of GSH, wherein when adsorption time is about 18h; Molecularly imprinted polymer is tending towards adsorption equilibrium to the unit adsorptive capacity of GSH, and is similar with the polymer property that adopts the preparation of function monomer methylacrylic acid; Template molecule gets in the molecularly imprinted polymer then needs the regular hour, if the time is too short, then molecularly imprinted polymer can not adsorb enough gsh; If adsorption time is oversize, it is free then can to lower efficiency even have the desorb of part gsh molecule; Therefore selecting 18-19h is preferable adsorption time, and selecting adsorption time is that 18h is the optimal adsorption time.
Comparative Examples 2: the preparation of blank polymkeric substance and application thereof
Get 5 parts of dimethylsulfoxide solvent, every part of 3ml is according to template molecule: function monomer=0: 2; 0: 4; 0: 5; 0: 8; 0: 10 mol ratio adds 0.4; 0.8; 1.0; 1.6; 2.0mmol function monomer acrylic amide AM; According to function monomer: linking agent=1: 5 (2.0; 4.0; 5.0; 8.0; 10.0mmol) molar ratio add the linking agent ethylene glycol dimethacrylate EGDMA of respective amount and initiator Diisopropyl azodicarboxylate AIBN.Ultrasonic degas 10min behind the ultrasonic mixing, feeds nitrogen 10min, in the state lower seal reaction system of nitrogen.60 ℃ of reaction 24h; Polyreaction is pulverized the synthetic polymkeric substance, grind, sieve after finishing, and removes template molecule with sodium chloride solution then, till detecting less than template molecule; The polymkeric substance of removing template molecule is carried out drying, obtain the molecularly imprinted polymer that size is 50-75 μ m at last.
Adopt blank polymkeric substance to carry out best sample solution pH, best sample solution concentration, the adsorption equilibrium time confirm that experimentation is with corresponding experimentation among the embodiment 2, the result shows that blank polymkeric substance does not have adsorptive power basically.
Therefore, the gsh molecularly imprinted polymer of the method preparation of the present invention through mass polymerization be block, passes through and pulverizes, uses after grinding, sieve, remove template molecule.The present invention is template molecule with the gsh; Prepare gsh molecularly imprinted polymer with specific selectivity; This molecularly imprinted polymer has specific " memory function " to template molecule; Template there is special affinity, has higher selectivity, realize separation gsh.Enrichment and purifying.
To sum up, gsh molecularly imprinted polymer of the present invention can separate gsh by specific selectivity, the enrichment gsh, and for subsequent applications provides the basis, its preparation method is simple and convenient, is suitable for large-scale promotion application.
In this specification sheets, the present invention is described with reference to its certain embodiments.But, still can make various modifications and conversion obviously and not deviate from the spirit and scope of the present invention.Therefore, specification sheets and accompanying drawing are regarded in an illustrative, rather than a restrictive.
Claims (10)
1. the preparation method of a gsh molecularly imprinted polymer; It is characterized in that; With the gsh is template molecule; Employing is used for the function monomer of gsh, thereby the said gsh that in the presence of linking agent and initiator, in shielding gas, carries out removing in the said polymkeric substance after polyreaction obtains polymkeric substance obtains said gsh molecularly imprinted polymer.
2. the preparation method of gsh molecularly imprinted polymer according to claim 1; It is characterized in that; Its concrete preparation process is: said gsh is dissolved in the solvent; Add said function monomer then and form template molecule-function monomer multipolymer, add said linking agent and said initiator again, in said shielding gas, carry out said polyreaction and obtain the said gsh that wash-out is removed in the said polymkeric substance behind the said polymkeric substance and obtain said gsh molecularly imprinted polymer.
3. the preparation method of gsh molecularly imprinted polymer according to claim 2; It is characterized in that; Said solvent is a methyl-sulphoxide, and said function monomer is methylacrylic acid or acrylic amide, and said linking agent is an ethylene glycol dimethacrylate; Said initiator is a Diisopropyl azodicarboxylate, and said shielding gas is a nitrogen.
4. the preparation method of gsh molecularly imprinted polymer according to claim 2 is characterized in that, said gsh passes through ultrasonic mixing in said solvent, the dissolving of spending the night, and concentration is 0.067～0.1mol/l.
5. the preparation method of gsh molecularly imprinted polymer according to claim 2; It is characterized in that; The molar ratio of said template molecule and said function monomer is 1: 2～1: 10; The molar ratio of said function monomer and said linking agent is 1: 4.5～5, and said initiator quality is about 1% of said function monomer quality.
6. the preparation method of gsh molecularly imprinted polymer according to claim 2 is characterized in that, said polyreaction was carried out 20-24 hour at 60-65 ℃.
7. the preparation method of gsh molecularly imprinted polymer according to claim 2 is characterized in that, employing concentration is that the sodium chloride solution wash-out of 0.4-0.8mol/l is removed the said gsh in the said polymkeric substance.
8. a gsh molecularly imprinted polymer is characterized in that, adopts the preparation method of the arbitrary described gsh molecularly imprinted polymer of claim 1-7 to be prepared from.
9. method that gsh molecularly imprinted polymer according to claim 8 is applied to separate gsh; It is characterized in that; The solution stream that will contain gsh is carried out the special absorption of selectivity through described gsh molecularly imprinted polymer, thus the said gsh of purifying.
10. method according to claim 9 is characterized in that, the described concentration that contains the solution of gsh is 1.5-2.0g/l, and pH is 4.8-5.3, and the Static Adsorption starting time of the special absorption of said selectivity is 18-19h.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103254355A (en) * | 2013-06-06 | 2013-08-21 | 贵州大学 | Carnosine molecularly imprinted polymer and preparation method and application thereof |
| CN103554329A (en) * | 2013-10-17 | 2014-02-05 | 江南大学 | Molecularly imprinted polymer based aloe-emodin removal technology |
| CN104407027A (en) * | 2014-12-16 | 2015-03-11 | 南京医科大学 | Method for preparing glutathione imprinted sensor through magnetic-control induced self-assembly and quick film formation |
| CN117165646A (en) * | 2023-08-25 | 2023-12-05 | 广州菲勒生物科技有限公司 | Collagen tripeptide composition and purification method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583804A (en) * | 2004-06-10 | 2005-02-23 | 中国农业大学 | Use and preparation of molecular track polymer of sulfonyl urea herbicide |
| US20100234565A1 (en) * | 2007-10-10 | 2010-09-16 | Polyintell | Molecularly imprinted polymers for the recognition of glutathione gsh, methods for preparing same and uses thereof |
-
2010
- 2010-12-03 CN CN2010105723649A patent/CN102485758A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583804A (en) * | 2004-06-10 | 2005-02-23 | 中国农业大学 | Use and preparation of molecular track polymer of sulfonyl urea herbicide |
| US20100234565A1 (en) * | 2007-10-10 | 2010-09-16 | Polyintell | Molecularly imprinted polymers for the recognition of glutathione gsh, methods for preparing same and uses thereof |
Non-Patent Citations (2)
| Title |
|---|
| AYSE DINCER ETAL: "PREPARATION OF GLUTATHIONE IMPRINTED POLYME", 《PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY》, no. 40, 8 July 2010 (2010-07-08), pages 188 - 197 * |
| 李琼: "谷胱甘肽分子印迹聚合物的合成及应用于荧光传感器膜的研究", 《工程科技I辑》, 21 March 2008 (2008-03-21), pages 014 - 86 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103254355A (en) * | 2013-06-06 | 2013-08-21 | 贵州大学 | Carnosine molecularly imprinted polymer and preparation method and application thereof |
| CN103554329A (en) * | 2013-10-17 | 2014-02-05 | 江南大学 | Molecularly imprinted polymer based aloe-emodin removal technology |
| CN104407027A (en) * | 2014-12-16 | 2015-03-11 | 南京医科大学 | Method for preparing glutathione imprinted sensor through magnetic-control induced self-assembly and quick film formation |
| CN117165646A (en) * | 2023-08-25 | 2023-12-05 | 广州菲勒生物科技有限公司 | Collagen tripeptide composition and purification method thereof |
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