CN102477450A - Method for preparing ethanol-type mixed culture biological hydrogen production system - Google Patents
Method for preparing ethanol-type mixed culture biological hydrogen production system Download PDFInfo
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- CN102477450A CN102477450A CN2010105589993A CN201010558999A CN102477450A CN 102477450 A CN102477450 A CN 102477450A CN 2010105589993 A CN2010105589993 A CN 2010105589993A CN 201010558999 A CN201010558999 A CN 201010558999A CN 102477450 A CN102477450 A CN 102477450A
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- ethanol
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/20—Sludge processing
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Abstract
The invention relates to a method for preparing an ethanol-type mixed culture biological hydrogen production system. The method comprises the following steps of: subjecting sludge to filtration, settlement and washing, culturing at room temperature under aerobic conditions, and loading to a reactor; diluting sucrose with water, and adding agricultural nitrogen-phosphorus compound fertilizers; and introducing the resulting mixed liquid to the reactor, adding alkalinity regulators and oxidation-reduction potential regulators, controlling water retention time to be 6 hours, and controlling temperature of the reactor within the range of 34 DEG C to 36 DEG C, to produce the ethanol-type mixed culture biological hydrogen production system. Compared with the prior art, the pH value of the reaction region is adjusted by the alkalinity regulators and the oxidation-reduction potential (ORP) of the reaction region is adjusted by the oxidation-reduction potential regulators, so as to rapidly set ecological niches suitable for ethanol-type fermentation bacteria. Besides, the method is significant in buffering property and stability, so that the system can rapidly and stably produce hydrogen by ethanol-type fermentation with high efficiency.
Description
Technical field
The present invention relates to a kind of preparation method of hydrogen generating system, especially relate to a kind of method of preparation of ethanol-type mixed culture biological hydrogen production system.
Background technology
Under the background of global energy crisis, biological hydrogen production technology can be utilized such as a series of renewable resources hydrogen producings in next life such as high concentrated organic wastewater, carbohydrate containing materials, so this technology becomes the key and research focus that solves energy problem.The anaerobic fermentation of biological process for making hydrogen be a kind of novel, less energy-consumption, the cleaning the changing rejected material to useful resource disposal options; Dark fermentation method biological hydrogen production mainly contains ethanol-type fermentation, the fermentation of butyric acid type and 3 kinds of fermented types of propionic acid type fermentation; The hydrogen production potential obvious difference of different fermentations type, wherein, ethanol-type fermentation has maximum hydrogen output and hydrogen-producing speed; Produce the hydrogen fermented type for the best, the control method of seeking this fermented type has great importance for the industrial applications of accelerating biological hydrogen production.Yet existing processes generally is to regulate pH and add FeCl through adding sodium bicarbonate
3Regulate the method for ORP and control, make the mixed culture biological hydrogen production system reach specific ethanol-type fermentation, this method resiliency and stable aspect be still waiting to strengthen.
Summary of the invention
The object of the invention is exactly to provide a kind of to produce that the hydrogen performance is good, the method for the preparation of the simple ethanol-type mixed culture of technology biological hydrogen production system in order to overcome the defective that above-mentioned prior art exists.
The object of the invention can be realized through following technical scheme:
A kind of method of preparation of ethanol-type mixed culture biological hydrogen production system is characterized in that, this method may further comprise the steps:
(1) with sludge of sewage treatment plants, after filtering, precipitate, eluriating, aeration was cultivated 10 days under the room temperature, then it was inserted in the reactor drum as kind of mud;
(2), to wherein adding agricultural compound nitrogen phosphate fertilizer, obtain mixing solutions then with the sucrose solution thin up;
(3) use constant flow pump that the mixing solutions that obtains in the step (2) is imported in the reactor drum; The pH value that adds solution in the alkaline conditioner controlling reactor then is 4.0～4.3; The redox potential that adds solution in the redox potential regulator control reactor drum is-240～-280mV; The control hydraulic detention time is 6h, and temperature of reactor is 34～36 ℃, and reaction obtains ethanol-type mixed culture biological hydrogen production system.
Described reactor drum is an even flow slot type stirred reactor.
COD (COD) concentration of the mixing solutions described in the step (2) is 5000～7000mg/L.
COD in the mixing solutions described in the step (2): the concentration ratio of N: P is 1000: 5: 1.
Alkaline conditioner described in the step (3) is NaHCO
3-Na
2CO
3Mixture.
Redox potential regulator described in the step (3) is halfcystine-xitix mixture.
Compared with prior art, the present invention has the following advantages:
(1) utilization " NaHCO
3-Na
2CO
3" mixture and " halfcystine-xitix " mixture can make environment of system reach specific niche scope in the short period of time, sets up ethanol-type fermentation;
(2) mixed culture biological hydrogen production system stability is strengthened, and has better product hydrogen performance;
(2) the mixed culture biological hydrogen production system has stronger shock-absorbing capacity to organic loading;
(3) the mix reagent raw material is easy to get;
(4) fermenting process is regulated simple.
Embodiment
Below in conjunction with specific embodiment the present invention is elaborated.
Embodiment 1
With the sugar beet juice is the ethanol-type fermentation technology of the mixed culture biological hydrogen production system of substrate, and this method comprises following process step:
(1) earlier with after active sludge filtration, deposition, the elutriation, carried out acclimation shaking culture about about 10 days, add sucrose raffinate (5000mg/L) and a certain proportion of agricultural composite fertilizer (4g) of The addition of C OD concentration between domestication, be seeded in the biological hydrogen production reactor then;
(2) the sucrose raffinate thin up of beet factory becomes the water inlet COD concentration (5000mg/L) in the process parameters range, adds a certain proportion of agricultural composite fertilizer (4g) simultaneously;
(3) use constant flow pump, the sucrose raffinate after the dilution is delivered to the even flow stirred autoclave, it is 6h that hydraulic detention time is set, and the temperature that heating keeps reaction kettle is at 35 ℃;
(4) add " NaHCO in the water inlet
3-Na
2CO
3" mixture, conditioned reaction district pH adds " halfcystine-xitix " mixture conditioned reaction district ORP simultaneously in 4.0～4.3 scopes, make it-250～-the 280mV scope;
(5) adopt gas Chromatographic Determination water outlet liquid phase end products component, confirm that fermentation system reaches ethanol-type fermentation, the gas of generation is through after being equipped with the air collector of sodium hydroxide saturated solution, and the gas behind the collection filter wash is hydrogen.
Embodiment 2
With the sugar beet juice is the ethanol-type fermentation technology of the mixed culture biological hydrogen production system of substrate, and this method comprises following process step:
(1) earlier with after active sludge filtration, deposition, the elutriation, carried out acclimation shaking culture about about 10 days, add sucrose raffinate (5000mg/L) and a certain proportion of agricultural composite fertilizer (4g) of The addition of C OD concentration between domestication, be seeded in the biological hydrogen production reactor then;
(2) the sucrose raffinate thin up of beet factory becomes the water inlet COD concentration (7000mg/L) in the process parameters range, adds a certain proportion of agricultural composite fertilizer (4g) simultaneously;
(3) use constant flow pump, the sucrose raffinate after the dilution is delivered to the even flow stirred autoclave, it is 6h that hydraulic detention time is set, and the temperature that heating keeps reaction kettle is at 35 ℃;
(4) add " NaHCO in the water inlet
3-Na
2CO
3" mixture, conditioned reaction district pH adds " halfcystine-xitix " mixture conditioned reaction district ORP simultaneously in 4.2～4.4 scopes, make it-240～-the 270mV scope;
(5) adopt gas Chromatographic Determination water outlet liquid phase end products component, confirm that fermentation system reaches ethanol-type fermentation, the gas of generation is through after being equipped with the air collector of sodium hydroxide saturated solution, and the gas behind the collection filter wash is hydrogen.
Embodiment 3
A kind of method of preparation of ethanol-type mixed culture biological hydrogen production system, this method may further comprise the steps:
(1) with sludge of sewage treatment plants, after filtering, precipitate, eluriating, aeration was cultivated 10 days under the room temperature, then it was inserted in the even flow slot type stirred reactor as kind of mud;
(2) with the sucrose solution thin up, to wherein adding agricultural compound nitrogen phosphate fertilizer, obtain mixing solutions then, the COD of this mixing solutions (COD) concentration is 6000mg/L, the concentration ratio of the COD in the mixing solutions: N: P is 1000: 5: 1;
(3) use constant flow pump that the mixing solutions that obtains in the step (2) is imported in the reactor drum, add NaHCO then
3-Na
2CO
3Mixture is 4.0 as the pH value of solution in the alkaline conditioner controlling reactor; Adding halfcystine-xitix mixture as the redox potential of solution in the redox potential regulator control reactor drum is-240mV; The control hydraulic detention time is 6h; Temperature of reactor is 36 ℃, and reaction obtains ethanol-type mixed culture biological hydrogen production system.
Embodiment 4
A kind of method of preparation of ethanol-type mixed culture biological hydrogen production system, this method may further comprise the steps:
(1) with sludge of sewage treatment plants, after filtering, precipitate, eluriating, aeration was cultivated 10 days under the room temperature, then it was inserted in the even flow slot type stirred reactor as kind of mud;
(2) with the sucrose solution thin up, to wherein adding agricultural compound nitrogen phosphate fertilizer, obtain mixing solutions then, the COD of this mixing solutions (COD) concentration is 6000mg/L, the concentration ratio of the COD in the mixing solutions: N: P is 1000: 5: 1;
(3) use constant flow pump that the mixing solutions that obtains in the step (2) is imported in the reactor drum, add NaHCO then
3-Na
2CO
3Mixture is 4.3 as the pH value of solution in the alkaline conditioner controlling reactor; Adding halfcystine-xitix mixture as the redox potential of solution in the redox potential regulator control reactor drum is-280mV; The control hydraulic detention time is 6h; Temperature of reactor is 34 ℃, and reaction obtains ethanol-type mixed culture biological hydrogen production system.
Claims (6)
1. the method for the preparation of an ethanol-type mixed culture biological hydrogen production system is characterized in that, this method may further comprise the steps:
(1) with sludge of sewage treatment plants, after filtering, precipitate, eluriating, aeration was cultivated 10 days under the room temperature, then it was inserted in the reactor drum as kind of mud;
(2), to wherein adding agricultural compound nitrogen phosphate fertilizer, obtain mixing solutions then with the sucrose solution thin up;
(3) use constant flow pump that the mixing solutions that obtains in the step (2) is imported in the reactor drum; The pH value that adds solution in the alkaline conditioner controlling reactor then is 4.0～4.3; The redox potential that adds solution in the redox potential regulator control reactor drum is-240～-280mV; The control hydraulic detention time is 6h, and temperature of reactor is 34～36 ℃, and reaction obtains ethanol-type mixed culture biological hydrogen production system.
2. the method for the preparation of a kind of ethanol-type mixed culture biological hydrogen production system according to claim 1 is characterized in that, described reactor drum is an even flow slot type stirred reactor.
3. the method for the preparation of a kind of ethanol-type mixed culture biological hydrogen production system according to claim 1 is characterized in that, COD (COD) concentration of the mixing solutions described in the step (2) is 5000～7000mg/L.
4. the method for the preparation of a kind of ethanol-type mixed culture biological hydrogen production system according to claim 1 is characterized in that, the COD in the mixing solutions described in the step (2): the concentration ratio of N: P is 1000: 5: 1.
5. the method for the preparation of a kind of ethanol-type mixed culture biological hydrogen production system according to claim 1 is characterized in that, the alkaline conditioner described in the step (3) is NaHCO
3-Na
2CO
3Mixture.
6. the method for the preparation of a kind of ethanol-type mixed culture biological hydrogen production system according to claim 1 is characterized in that, the redox potential regulator described in the step (3) is halfcystine-xitix mixture.
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| CN2010105589993A CN102477450A (en) | 2010-11-25 | 2010-11-25 | Method for preparing ethanol-type mixed culture biological hydrogen production system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111041051A (en) * | 2019-11-25 | 2020-04-21 | 浙江大学 | Method for promoting anaerobic fermentation to produce methane by degrading waste oil through active free radical broken bond |
-
2010
- 2010-11-25 CN CN2010105589993A patent/CN102477450A/en active Pending
Non-Patent Citations (5)
| Title |
|---|
| LI YONG-FENG等: "Review on engineering of fermentative biohydrogen production", 《哈尔滨商业大学学报(自然科学版)》 * |
| 李永峰等: "利用OLR和pH调控快速建立生物制氢反应器", 《太阳能学报》 * |
| 李永峰等: "生物制氢系统产氢菌的富集培养与分离技术", 《南京理工大学学报》 * |
| 王勇等: "乙醇型发酵与丁酸型发酵产氢机理及能力分析", 《太阳能学报》 * |
| 韩伟等: "底物浓度对连续流发酵制氢系统的影响", 《中国环境科学学会学术年会论文集》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111041051A (en) * | 2019-11-25 | 2020-04-21 | 浙江大学 | Method for promoting anaerobic fermentation to produce methane by degrading waste oil through active free radical broken bond |
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Application publication date: 20120530 |