CN102462694A - Application of hydrogen to preparation of medicament and health-care beverage for preventing kidney stones - Google Patents
Application of hydrogen to preparation of medicament and health-care beverage for preventing kidney stones Download PDFInfo
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- CN102462694A CN102462694A CN2010105416439A CN201010541643A CN102462694A CN 102462694 A CN102462694 A CN 102462694A CN 2010105416439 A CN2010105416439 A CN 2010105416439A CN 201010541643 A CN201010541643 A CN 201010541643A CN 102462694 A CN102462694 A CN 102462694A
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Abstract
The invention provides an application of hydrogen to preparation of a medicament and a health-care beverage for preventing kidney stones. Hydrogen is prepared into an oral preparation or beverage; after the oral preparation or the beverage is taken, oxalic acid output can be effectively lowered, and urine citric acid concentration is increased; and meanwhile, SOD (Super Oxygen Dehydrogenises) activity can be increased, the generation of MDA (Methane Dicarboxylic Aldehyde) and formation of calcium oxalate stones are reduced, and the reoccurrence of calcium oxalate stones is prevented.
Description
Technical field
The present invention relates to a kind of new application of hydrogen, relate in particular to a kind of hydrogen in the medicine of preparation prevention renal calculus or the application in the health promoting beverage.
Background technology
Kidney belongs to the part of urinary system; Be responsible for impurity, discharge metabolic waste poisonous substance and the medicine in the filtering blood and keep body fluid and electrolytical balance; Simultaneously also possess endocrine function with blood pressure regulation, the traditional Chinese medical science is thought as the kidney controlling essence storage of one of the five internal organs, main development growth and reproduction; Being the root of the congenital foundation and life, is human body and important internal organs thereof.
Renal calculus is a kind of commonly encountered diseases of kidney, the increase of Along with people's animal proteinum, salt and saccharide intake, and the renal calculus sickness rate also obviously improves; Calculus often is attended by the violent pain unbearably of the paroxysmal that breaks out in case formation can produce very havoc property to kidney, and calculus is heavier to mucosa injury simultaneously, so gross hematuria often occurs; And renal calculus also can cause complication such as urinary system is blocked, anemia, have a strong impact on patient's body health, and bring great misery to the patient; Phenomenons such as renal calculus severe patient even generation anuria, renal failure, toxic shock, in addition dead.
Though summed up many experiences and method people aspect the treatment renal calculus, renal calculus is treated after occurring again, brings many miseries to the patient first; And had a strong impact on the health of health; Second the patient also will spend great amount of time and fund is treated, and have a strong impact on live and work, and many Therapeutic Method such as also can destroy human electrolyte's balance or cause that rubble blocks at side effect; Cause hydronephrosis or renal failure, and very easily recurrence.Much get the stone operation and have certain risk, severe complication possibly occur, even take place dead.Therefore, the prevention of renal calculus is extremely important.
At present the prevention about renal calculus mainly is some common senses, as eat less salt with sugar, control proteinic absorption, polydipsia water or the like, be difficult to provide a kind of prevention method of effectively, operability to people.
Patent CN1230195C discloses a kind of oral formulations that contains Sargassum that can prevent renal calculus, and main component is tens kinds of biological extraction compositions such as Ascophyllum nodosum Sargassum, mannitol, nicotinic acid, tocopherol; Patent CN1739679B discloses a kind of Chinese medicine that can be used for treating and preventing renal calculus; Comprise tens kinds of Chinese crude drugs such as Fructus Lycii, Spica Prunellae, Pheretima, yellow Stilbene, Endothelium Corneum Gigeriae Galli, above-mentioned two kinds of patents often receive the influence of raw material sources owing to be too dependent on bio-extract; Cause the raw material storage cost higher; Final product price also can correspondingly improve, and composition is too much, prepares loaded down with trivial details.
Patent CN1130993C discloses a kind of ginger ale, and Rhizoma Zingiberis steaming and decocting, squeezing after-filtration are made Sucus Zingberis, is mixed and made into the beverage with prevention renal calculus effect with other components again, because Sucus Zingberis has stronger zest taste, so much human is difficult to swallow.
Patent WO2007/075447A2 discloses a kind of granule that comprises oxalate degrading enzyme; Can be used for treatment and the special property sent out of prevention calcium oxalate renal calculus; And obtained good effect, but organized enzyme is preserved difficulty, and cost is too high; And this granule is only limited to the prevention calcium oxalate stone, do not have effect for calcium phosphate stone, urate calculus, magnesium ammonium phosphate calculus or cystine stone etc.
It is believed that all the time hydrogen is the same with helium, belong to physiological inert gas, not with human body in any material react; But as far back as 1975; Someone publishes thesis on " science " magazine, and can effectively to treat the animal skin malignant melanoma plain for proof hydrogen, and calendar year 2001, France submarine medicine man confirmed that hydrogen can be treated the liver schistosoma mansoni and infected the inflammatory reaction that causes; Belong to the traditional view of noble gas though hydrogen has been overthrown in above-mentioned research, and fail to cause people's attention.And hydrogen does not have report as yet in the research aspect the treatment renal calculus.Particularly there is explosion danger in high pressure hydrogen and other gas mixed process, also is difficult to as the GCS means.
Summary of the invention
The invention provides the application of a kind of hydrogen in preparation prevention renal calculus medicine and health promoting beverage; Through physics or chemical method hydrogen is dissolved in and to process oral formulations or beverage in the drinkable liquid composition; Confirm through biotic experiment; Take after the medicine or health promoting beverage of the present invention's preparation, SOD is active in citric acid excretion and the nephridial tissue in the urine obviously increases, and the MDA level obviously reduces in urine oxalic acid excretion and the nephridial tissue; The generation of renal calculus obviously reduces, and explains that the medicine or the beverage of the present invention's preparation can effectively prevent renal calculus.
The object of the present invention is to provide a kind of new application of hydrogen, especially the application in preparation prevention renal calculus medicine and health promoting beverage.
Wherein, hydrogen is dissolved in the drinkable liquid, process the oral liquid or the health promoting beverage that contain hydrogen then through physics or chemical method.
Preferably, said oral liquid solvent comprises water, G/W, normal saline etc., can be one or several mixing wherein.Can also add the available adjuvant of pharmaceutical field.
Further, when oral liquid or the beverage of preparation hydrogen, with the oxidation reduction potential control of said oral liquid or beverage≤-50mV, density of hydrogen>=0.08ppm.
Wherein, said beverage is a kind of in soda pop, fruit juice and vegetable juice, protein beverage, drinking water, tea beverage, coffee beverage or the plant beverage.Wherein said plant beverage refers to: with plant or plant extract (except tea, the coffee) is that raw material (root, stem, leaf, flower, seed and bamboo of non-fruit and vegerable class plant or the excretory juice of trees self are like corn, edible fungi, edible algae and fern, cocoa, Flos Chrysanthemi etc.) is through the beverage product of processing or fermentation is processed.
The present invention adopts the active component of hydrogen as prevention renal calculus medicine and health promoting beverage, because hydrogen has advantages such as low price, good stability, nontoxic, noresidue, the medicine or the health promoting beverage cost of the hydrogen that therefore prepares are low.In Clinical Application a kind of new selection is provided for hydrogen simultaneously.
Hydrogen is dissolved in the liquid component, can processes the oral formulations or the beverage that are similar to soda pop, do not need high pressure hydrogen, hydrogen promptly can not occur and mix with other gases and cause the problem of exploding.And drink conveniently, can be used as the daily beverage of people.
Find through luring the stone animal model experiment; Take after the medicine or health promoting beverage that contain hydrogen of the present invention's preparation; Urine oxalic acid excretion is reduced to about 13 μ mol/24h by 18 μ mol/24h; Explain that hydrogen can effectively reduce the urine concentration of oxalic acid, avoid forming calcium oxalate crystal, and calcium oxalate crystal is to cause the main arch-criminal of renal calculus; Simultaneously the citric acid excretion is promoted to about 50 μ mol/24h by 30 μ mol/24h, explain that hydrogen can effectively increase to urinate the citric acid excretion, and the latter can effectively suppress calcium oxalate crystal mutual gathering, grow into calculus.Take the medicine and the beverage of the present invention's preparation; Make nephridial tissue malonaldehyde (MDA) level drop to 4.0 nmol/mg by 6.3 nmol/mg; Nephridial tissue superoxide dismutase (SOD) is active to be increased to about 200U/mg by 151U/mg; With the section of polarized light microscope observing kidney, after finding to take the medicine and beverage that contain hydrogen of the present invention's preparation, calcium oxalate crystal obviously reduces simultaneously.The medicine that contains hydrogen of above-mentioned statement of facts the present invention preparation and health promoting beverage have the effect of tangible prevention renal calculus and protection kidney.
Description of drawings
Fig. 1 is a zoopery A group pathological section photo under the polarizing microscope;
Fig. 2 is a zoopery B group pathological section photo under the polarizing microscope;
Fig. 3 is a zoopery C group pathological section photo under the polarizing microscope;
Fig. 4 is the horizontal comparison diagram of zoopery MDA;
Fig. 5 is the active comparison diagram of zoopery SOD;
Fig. 6 is a zoopery concentration of oxalic acid comparison diagram;
Fig. 7 is 24 hours oxalic acid excretions of zoopery comparison diagram.
The specific embodiment
Embodiment 1:
Generating hydrogen with water and magnesium metal reaction is example, and hydrogen is dissolved in the water, processes the oral liquid that contains hydrogen, and control oral liquid oxidation-reduction potential is-50mV that density of hydrogen is 0.08ppm.Carry out zoopery with prepared oral liquid then.
Select the health cleaning level Thirty male rats 24 (available from Shanghai Slac Experimental Animal Co., Ltd.) of body weight between 180g ~ 220g for use, be divided into ethylene glycol at random and lure stone+oral liquid intervention group (A group), simple ethylene glycol to lure stone group (B group) and blank group (C group).Three groups under same environment adaptability feed a week, handling 4 weeks: A group by following method respectively subsequently is unique drinking water source with 1% ethylene glycol solution, irritates the hydrogen oral liquid once (4ml/ is only) of feeding prepared fresh simultaneously every day; The B group is unique drinking water source with 1% ethylene glycol, irritates every day simultaneously and feeds deionized water once (4ml/ only); The C group is unique drinking water source with deionized water.
Detection method and detection index:
Handling the 29th day changes rat over to metabolic cage, collects every rat twenty-four-hour urine liquid respectively.Be divided into three parts after the metering, measure oxalates, citrate and calcium ion content respectively.Rat dislocation execution was cut kidney, the nephridial tissue paraffin section in the 30th day; 200 times of polarized light microscope observing nephridial tissue mesoxalic acid calcium calculus situation, and press document " influence that coumarin forms the experimental rat calcium oxalate stone " (An Ruihua, Feng Tao; Guo Yinglu etc., Chinese magazine of urology surgery, 1994; 15:209-211) standard grading is measured active and malonaldehyde (MDA) level of superoxide dismutase in the nephridial tissue (SOD) simultaneously.
Statistical analysis:
Analyze with the SPSS13.0 statistical software, average is relatively used variance analysis between the measurement data group: test of normality is at first carried out in (1); (2) if data meets normality (be P>0.05), then carry out the homogeneity of variance analysis, if the data homogeneity of variance (be P>0.05), then carry out variance analysis check (being the F check).If the variance analysis assay is (be P>0.05) significantly, then further carry out multiple comparisons and check with SNK parametric test method; For ranked data, then adopt the DMH X 2 test.Testing result is seen table 1, table 2 and table 3 and Fig. 1, Fig. 2 and Fig. 3.
Can find out from table 1; Taking the A group of the oral liquid of the present invention's preparation compares with luring stone group B group merely; Urine concentration of oxalic acid and oxalic acid excretion all have obvious decline (with reference to Fig. 6 and Fig. 7), and urine citric acid concentration and citric acid excretion all are significantly improved, and explain that hydrogen can effectively reduce the urine oxalic acid content and increase urine citric acid content; Urine oxalic acid is one of topmost forming factors of renal calculus, and the urine citric acid then is one of topmost inhibition factor of renal calculus.Therefore the present invention can effectively prevent the formation of renal calculus.
Table 1, physical and chemical index (unit: concentration mmol/L, excretion μ mol/24h)
The nephridial tissue pathological section shows under 200 times of polarizing microscopes: C group cell marshalling, rule, and clear in structure is not seen tubular ectasia and calcium oxalate crystal deposition (with reference to Fig. 3); B group tubular ectasia is serious, tangible crystallization deposition is arranged in the tube chamber, and be gathered into calculus, part tube chamber total blockage (with reference to Fig. 2); And A group part renal tubules tube chamber has only the small amount of crystalline deposition, and the comparatively dispersion that distributes, and does not have agglomerating phenomenon (with reference to Fig. 1).Table 3 is seen in three groups of renal tubules calcium oxalate crystal scorings.
Table 3, the scoring of renal tubules calcium oxalate crystal
Under normal conditions, the urine stream in the renal tubules is very fast, and newborn free calcium oxalate crystal can't be stopped to grow up to calculus.Because oxalic acid is except forming calcium oxalate crystal, after be gathered into each other outside the renal calculus, also can be through oxidation reaction damage renal cells.Urine oxalic acid can obviously increase the weight of the nephrocyte response to oxidative stress; Cause lipid peroxidation, DNA oxidation and the mitochondrial depolarization of cell membrane and subcellular structure; And then cause the change of cellularity function, and causing apoptosis at last and come off, the below basement membrane exposes; Calcium oxalate crystal in the urine just can adhere on the wall and be able to be stranded in the renal tubules intracavity, assembles subsequently, grows and form calculus.The detection and the polarized light microscope observing result of each group of contrast find that along with the urine concentration of oxalic acid increases, antioxidase material SOD obviously reduces in the nephridial tissue, tubular ectasia, and little calculus increases in the tube chamber, and this also confirms the destruction of oxalic acid to renal cells.
Intracellular reactive chalcogen (ROS) or free-radical generating excessively are the root places of oxidative damage, and wherein hydroxyl is active the strongest in the active chalcogen, can non-selectivity ground and nucleic acid, lipid, albumino reaction, produce cytotoxicity.Hydrogen has good after birth penetrance, in vivo under the effect of ubiquitous hydrogenase, optionally react and generate water with having Cytotoxic hydroxyl, but with other functional groups reactions, anti-oxidation efficacy is desirable.
Therefore the oral liquid and the beverage of the hydrogen of the present invention's preparation can effectively reduce the urine concentration of oxalic acid; Reduce the generation of calcium oxalate crystal; It is active and reduce MDA level (seeing table 2) that simultaneously the selective antioxidation of hydrogen can promote SOD, effectively protects renal cells, calcium oxalate crystal is difficult for adhering on the tube wall and is prone to washed away by urine stream; Be difficult to assemble, grow up, thereby further prevent the formation of calculus.
The oral liquid of above-mentioned experimental result proof hydrogen of the present invention can effectively stop the formation of calcium oxalate crystal, effectively stops adhesion, gathering and the growth of calcium oxalate crystal to the renal tubules inwall, aspect the prevention renal calculus obvious effects is being arranged.
Embodiment 2:
With reference to embodiment 1, pressurized with hydrogen is dissolved in the milk, process the milk that contains hydrogen, the oxidation reduction potential control of made milk≤-50mV.
Embodiment 3:
With reference to embodiment 1, pressurized with hydrogen is dissolved in the fruit juice, process the fruit drink that contains hydrogen, the oxidation reduction potential control of made fruit drink≤-50mV.
When dissolving in hydrogen in milk or the fruit juice, can also add flavoring agent, process the beverage of various tastes, satisfy the daily mouthfeel preference of drinking of people, also play the effect of prevention renal calculus simultaneously.
Embodiment 4:
With reference to embodiment 1, pressurized with hydrogen is dissolved in the glucose solution, add the available adjuvant of pharmaceutical field, process the glucose oral liquid that contains hydrogen, the oxidation reduction potential control of made oral liquid is at-75mV.
Embodiment 5:
With reference to embodiment 1, pressurized with hydrogen is dissolved as in the mineral water, process the mineral water that contains hydrogen, the oxidation reduction potential control of made mineral water≤-100mV.
The invention provides the application of a kind of hydrogen in preparation prevention renal calculus medicine and health promoting beverage; Hydrogen is processed oral formulations or beverage; The concentration that reduces urine oxalic acid can be arranged after taking; Obviously remove strong oxidation activity oxygen such as hydroxyl, alleviate the response to oxidative stress of oxalic acid, the protection renal cells renal cells.Both reduced the generation of calcium oxalate crystal, prevented again that calcium oxalate crystal from renal tubules inwall tactophily, effectively having prevented the formation of renal calculus,, and a kind of new scheme was provided for the clinical practice of hydrogen for hydrogen provides a kind of new application.
More than specific embodiment of the present invention is described in detail, but it is just as example, the present invention is not restricted to the specific embodiment of above description.To those skilled in the art, any equivalent modifications that the present invention is carried out with substitute also all among category of the present invention.Therefore, not breaking away from impartial conversion and the modification of being done under the spirit and scope of the present invention, all should contain within the scope of the invention.
Claims (3)
1. the application of hydrogen in preparation prevention renal calculus medicine and health promoting beverage; It is characterized in that; Through physics or chemical method hydrogen is dissolved in the drinkable liquid; Process oxidation-reduction potential≤-drinkable liquid of 50mV, hydrogen content>=0.08ppm, then said liquid dosage is become to contain the oral liquid or the health promoting beverage of active component hydrogen.
2. the application of hydrogen according to claim 1 in preparation prevention renal calculus medicine and health promoting beverage is characterized in that, the solvent of said oral liquid is one or several the mixing in water, G/W, normal saline, the acetic acid.
3. the application of hydrogen according to claim 1 in preparation prevention renal calculus medicine and health promoting beverage; It is characterized in that said beverage is a kind of in soda pop, fruit juice and vegetable juice, protein beverage, drinking water, tea beverage, coffee beverage or the plant beverage.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103919234A (en) * | 2014-04-30 | 2014-07-16 | 苏州驰亚机电科技股份有限公司 | Hydrogen-enriched drink preparation method and equipment |
| CN106085689A (en) * | 2015-09-28 | 2016-11-09 | 上海金苇子生物技术有限公司 | A kind of beverage and preparation method thereof |
| CN112739724A (en) * | 2018-07-23 | 2021-04-30 | 纽崔吉诺姆有限责任公司 | Generation and maintenance of molecular hydrogen (H) in aqueous systems2) Compositions and methods of |
| CN114948989A (en) * | 2021-02-19 | 2022-08-30 | 中国人民解放军海军军医大学 | Application of magnesium hydride in preparing medicine for preventing and treating kidney crystal lithiasis |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101253973A (en) * | 2008-01-23 | 2008-09-03 | 王峰 | Adding hydrogen and deuterium drinking water and preparation thereof |
-
2010
- 2010-11-12 CN CN2010105416439A patent/CN102462694A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101253973A (en) * | 2008-01-23 | 2008-09-03 | 王峰 | Adding hydrogen and deuterium drinking water and preparation thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103919234A (en) * | 2014-04-30 | 2014-07-16 | 苏州驰亚机电科技股份有限公司 | Hydrogen-enriched drink preparation method and equipment |
| CN103919234B (en) * | 2014-04-30 | 2016-05-18 | 苏州驰亚机电科技股份有限公司 | A kind of rich hydrogen method for preparing beverage and equipment |
| CN106085689A (en) * | 2015-09-28 | 2016-11-09 | 上海金苇子生物技术有限公司 | A kind of beverage and preparation method thereof |
| CN112739724A (en) * | 2018-07-23 | 2021-04-30 | 纽崔吉诺姆有限责任公司 | Generation and maintenance of molecular hydrogen (H) in aqueous systems2) Compositions and methods of |
| EP3827027A4 (en) * | 2018-07-23 | 2022-04-27 | Nutragenom, LLC | Composition and methods for generating and sustaining molecular hydrogen (h2) in aqueous systems |
| US11318160B2 (en) | 2018-07-23 | 2022-05-03 | Nutragenom, Llc | Composition and methods for generating and sustaining molecular hydrogen (H2) in aqueous systems |
| CN114948989A (en) * | 2021-02-19 | 2022-08-30 | 中国人民解放军海军军医大学 | Application of magnesium hydride in preparing medicine for preventing and treating kidney crystal lithiasis |
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Application publication date: 20120523 |