CN102450269A - Triazolone nanometer colloid suspending agent and preparation method thereof - Google Patents
Triazolone nanometer colloid suspending agent and preparation method thereof Download PDFInfo
- Publication number
- CN102450269A CN102450269A CN2011104458034A CN201110445803A CN102450269A CN 102450269 A CN102450269 A CN 102450269A CN 2011104458034 A CN2011104458034 A CN 2011104458034A CN 201110445803 A CN201110445803 A CN 201110445803A CN 102450269 A CN102450269 A CN 102450269A
- Authority
- CN
- China
- Prior art keywords
- triazolone
- suspending agent
- nano
- preparation
- colloid suspending
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a triazolone nanometer biological agent and a preparation method thereof. The triazolone nanometer biological agent is prepared by an ultrasonic wave method and a crushing method. The prepared triazolone nanometer biological agent is particles of nanograde, and the particle size of the particles is 80-500nm; and the triazolone nanometer biological agent keeps the sterilization effect of paclobutrazol (PP333), enhances the effect of the triazolone, and has excellent bacteriostasis when being used in crops such as wheat, Chinese cabbage, rape and the like.
Description
Technical field
The present invention relates to a kind of triazolone nano-colloid suspending agent.
Background technology
Triazolone can make the synthetic inhibition of disease fungus cell membrane ergosterol.In the germ body, be reduced into " Triadimenol " and increased virulence.Epidemic disease to Oomycete mould (not producing ergosterol) is invalid.It is a kind of have strong in the bactericide of absorption, have two-way conduction function, and have prevention, root out, treatment and fumigation action, the lasting period is longer; Also be a kind of efficient, low toxicity, low-residual, the lasting period is long, interior absorption is strong triazole bactericidal agent.After the each several part of plant absorption, can in plant corpus, conduct.Rust and powdery mildew are had prevention, effect such as root out, treat, fumigate.All effective to the disease of various crop such as corn circle pinta, wheat class moire disease, wheat leaf rot, pineapple black rot, maize head smut etc.Safer to fish and birds.Harmless to honeybee and natural enemy.The bactericidal mechanism principle of triazolone is very complicated, mainly is the biosynthesis that suppresses the thalline ergosterol, thereby the growth, the growth of mycelia and the formation of spore that suppress or disturb thalline to adhere to spore and haustorium.Triazolone is very strong to some germ activity in live body, but the poor that exsomatizes.Specific activity to mycelia is strong to spore.Triazolone can with many bactericide.Insecticides (tech) & Herbicides (tech)s etc. are now-making-now-using at present.Nanometer technology combines with biotechnology, and is applied to biological field, has just formed a kind of new multidisciplinary interleaving techniques--nanometer biotechnology.One of nanometer biotechnology is the emerging field of development just gradually, and along with the new results in this field continue to bring out, the research range of nanometer technology and intension will constantly extend and enrich, and form a new subject gradually.The research range of nanometer biotechnology relates generally to nano meter biomaterial, Nano medication and fields such as gene delivery nano-carrier, nano biological sensor and nanometer formulation.
Summary of the invention
The purpose of this invention is to provide a kind of triazolone nano-colloid suspending agent and preparation method thereof.
The preparation method of triazolone nano biological preparation of the present invention is characterized in that, comprises the steps:
1. the 40mg triazolone is dissolved in the 10mL acetone;
2. the 10mg dodecyl sodium sulfate is mixed with 20mL, 70 ℃ of distilled water, stir 3min;
3. the mixed liquor that 1. step is obtained join step 2. in;
4. add the 20mg carboxyrnethyl starch sodium to step in 3., distilled water is settled to 100mL, stirs 17min;
5. after pulverizing 20min, ultrasonic 15min～30min;
6. add Hydroxypropyl methylcellulose 10mg, continue ultrasonic 10min.
In the above-mentioned course of reaction, the Hydroxypropyl methylcellulose preferred viscosity of step in 6. is 11～20cps.Step ultrasonic time 5. is preferably 20min; The frequency of excusing from death is preferably 59KHz.
Also should comprise the constant volume to triazolone nano-colloid suspending agent among the preparation method of above-mentioned triazolone nano biological preparation, those skilled in the art can prepare the triazolone nano-colloid suspending agent of variable concentrations as required.
Another object of the present invention is to provide by the prepared triazolone nano-colloid suspending agent of above-mentioned arbitrary method.
Triazolone nano-colloid suspending agent of the present invention is nano level particle, and its grain diameter is at 80～500nm.
Triazolone nano-colloid suspending agent of the present invention has not only kept the bactericidal action of paclobutrazol (PP333), and has strengthened the action effect of triazolone, is applied on the crops such as wheat Chinese cabbage, rape to have good bacteriostasis.
Description of drawings
Accompanying drawing 4 width of cloth of the present invention,
Fig. 1 is the picture of former dose of triazolone;
Fig. 2 is the picture that the light microscope of the embodiment of the invention 1 is observed down;
Fig. 3 is the picture of the bacteriostatic experiment of the embodiment of the invention 3;
Fig. 4 is the picture of the bacteriostatic experiment of Comparative Examples 1 of the present invention.
Embodiment
Following non-limiting example can make those of ordinary skill in the art more fully understand the present invention, but does not limit the present invention in any way.Triazolone used in the present invention derives from sky, Shandong nation, 15% trazodone wettable powder; Carboxyrnethyl starch sodium derives from Huzhou Zhanwang Pharmaceutical Co., Ltd.; The Hydroxypropyl methylcellulose model is E-15, purchases in Huzhou Zhanwang Pharmaceutical Co., Ltd.; Employed magnetic stirring apparatus is the 85-2 type constant temperature blender with magnetic force of giving magnificent instrument responsibility Co., Ltd; The ultrasonic cleaning appearance is Shanghai High Kudos Science Instrument Co., Ltd.; Light microscope is Nikon 50i.Penicillium Penicillium.
Embodiment 1
1. the 40mg triazolone is dissolved in the 10mL acetone;
2. the 10mg dodecyl sodium sulfate is mixed with 20mL, 70 ℃ of distilled water, stir 3min;
3. the mixed liquor that 1. step is obtained join step 2. in;
4. add the 20mg carboxyrnethyl starch sodium to step in 3., distilled water is settled to 100mL, stirs 17min;
5. after pulverizing 20min, ultrasonic 20min;
6. add the 10mg Hydroxypropyl methylcellulose, continue ultrasonic 10min.
The concentration of the triazolone nano-colloid suspending agent for preparing is 0.4g/L.The picture of under light microscope, observing as shown in Figure 1; Fig. 1 is the picture of former dose of triazolone; Fig. 2 is the picture of triazolone nano-colloid suspending agent, can find out that the grain diameter of the triazolone nano-colloid suspending agent of present embodiment is significantly less than the grain diameter of former dose of triazolone.
Embodiment 2
The colloid test:
Tyndall effect:
Measure the triazolone nano-colloid suspending agent 1mL of embodiment 1 preparation, be settled to 100ml, use laser radiation, in the triazolone nano-colloid suspending agent light path is arranged.Experimental result shows that triazolone nano-colloid suspending agent produces Tyndall effect.
The coagulation phenomenon:
Measure the triazolone nano-colloid suspending agent 10mL of embodiment 1 preparation, be settled to 100ml, add the FeCl of 1mL, 2mol/L
3Solution.Flocculent deposit occurs in the observation triazolone nano-colloid suspending agent, experimental result shows triazolone nano-colloid suspending agent generation coagulation phenomenon.
Embodiment 3
Bacteriostatic experiment:
1.. measure the triazolone nano-colloid suspending agent 1mL of embodiment 1 preparation, be settled to 10mL, promptly concentration is 40mg/L.
2.. by alcolhol burner, being coated with two concentration respectively is 10
-4Penicillium notatum dull and stereotyped.
3.. on superclean bench, the filter paper that to get two diameters that dip in triazolone nano-colloid suspending agent be 1cm is attached to respectively on two flat boards.
4.. place 35 ℃ of insulating boxs to cultivate 24h two flat boards.
5.. take out two flat boards, observe the inhibition zone size.
Experimental result: two antibacterial circle diameter sizes of flat board that triazolone nano-colloid suspending agent is handled are respectively 1.8cm, 2.2cm, average diameter 2.0cm.As shown in Figure 3.
Comparative Examples 1
Bacteriostatic experiment:
According to the method for operating of embodiment 3, carry out antibacterial for former dose with triazolone.
Experimental result: two antibacterial circle diameter sizes of the flat board of former dose of processing of triazolone are respectively 1.2cm, 1.4cm, average diameter 1.3cm.As shown in Figure 4.
Contrast through embodiment 3 and Comparative Examples 1 can find out that the nano-colloid suspending agent nano-colloid suspending agent processed group fungistatic effect of triazolone nano-colloid suspending agent obviously is better than the fungistatic effect of former dose of triazolone.
Claims (6)
1. the preparation method of a triazolone nano-colloid suspending agent is characterized in that, comprises the steps:
1. the 40mg triazolone is dissolved in the 10mL acetone;
2. the 10mg dodecyl sodium sulfate is mixed with 20mL, 70 ℃ of distilled water, stir 3min;
3. the mixed liquor that 1. step is obtained join step 2. in;
4. add the 20mg carboxyrnethyl starch sodium to step in 3., distilled water is settled to 100mL, stirs 17min;
5. after pulverizing 20min, ultrasonic 15min～30min;
6. add Hydroxypropyl methylcellulose 10mg, continue ultrasonic 10min.
2. the preparation method of triazolone nano-colloid suspending agent according to claim 1 is characterized in that the viscosity of the Hydroxypropyl methylcellulose during described step 6. is 11～20cps.
3. the preparation method of triazolone nano-colloid suspending agent according to claim 1 and 2 is characterized in that the ultrasonic time during described step 5. is 20min.
4. the preparation method of triazolone nano-colloid suspending agent according to claim 3, it is characterized in that described ultrasonic be to be to carry out under the 59KHz in frequency.
5. the triazolone nano-colloid suspending agent of method preparation according to claim 1.
6. the grain diameter of triazolone nano-colloid suspending agent according to claim 5 is 80～500nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104458034A CN102450269A (en) | 2011-12-27 | 2011-12-27 | Triazolone nanometer colloid suspending agent and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104458034A CN102450269A (en) | 2011-12-27 | 2011-12-27 | Triazolone nanometer colloid suspending agent and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102450269A true CN102450269A (en) | 2012-05-16 |
Family
ID=46034248
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011104458034A Pending CN102450269A (en) | 2011-12-27 | 2011-12-27 | Triazolone nanometer colloid suspending agent and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102450269A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103027035A (en) * | 2012-12-28 | 2013-04-10 | 辽宁师范大学 | Compound sodium nitrophenolate nano preparation and preparation method |
| CN103053513A (en) * | 2012-12-28 | 2013-04-24 | 辽宁师范大学 | Pyraclostrobin nano-preparation and preparation method thereof |
| CN103704242A (en) * | 2013-12-31 | 2014-04-09 | 辽宁师范大学 | Preparation method for multiple-effect nano biological composite regulating agent |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101692808A (en) * | 2009-09-30 | 2010-04-14 | 深圳诺普信农化股份有限公司 | Emamectin benzoate solid lipid nanoparticle and preparation method and application thereof in pesticide formulation |
| CN101755738A (en) * | 2009-11-11 | 2010-06-30 | 深圳诺普信农化股份有限公司 | Solid lipid nano spinosad and preparation method and application thereof |
| CN101801342A (en) * | 2007-07-31 | 2010-08-11 | 大塚制药株式会社 | methods for producing aripiprazole suspension and freeze-dried formulation |
| CN101909733A (en) * | 2007-12-28 | 2010-12-08 | 金伯利-克拉克环球有限公司 | Ultrasonic treatment chamber for particle dispersion into formulations |
| CN101946765A (en) * | 2010-02-26 | 2011-01-19 | 江苏剑牌农药化工有限公司 | Formulation and preparation method of triadimefon suspension |
-
2011
- 2011-12-27 CN CN2011104458034A patent/CN102450269A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101801342A (en) * | 2007-07-31 | 2010-08-11 | 大塚制药株式会社 | methods for producing aripiprazole suspension and freeze-dried formulation |
| CN101909733A (en) * | 2007-12-28 | 2010-12-08 | 金伯利-克拉克环球有限公司 | Ultrasonic treatment chamber for particle dispersion into formulations |
| CN101692808A (en) * | 2009-09-30 | 2010-04-14 | 深圳诺普信农化股份有限公司 | Emamectin benzoate solid lipid nanoparticle and preparation method and application thereof in pesticide formulation |
| CN101755738A (en) * | 2009-11-11 | 2010-06-30 | 深圳诺普信农化股份有限公司 | Solid lipid nano spinosad and preparation method and application thereof |
| CN101946765A (en) * | 2010-02-26 | 2011-01-19 | 江苏剑牌农药化工有限公司 | Formulation and preparation method of triadimefon suspension |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103027035A (en) * | 2012-12-28 | 2013-04-10 | 辽宁师范大学 | Compound sodium nitrophenolate nano preparation and preparation method |
| CN103053513A (en) * | 2012-12-28 | 2013-04-24 | 辽宁师范大学 | Pyraclostrobin nano-preparation and preparation method thereof |
| CN103704242A (en) * | 2013-12-31 | 2014-04-09 | 辽宁师范大学 | Preparation method for multiple-effect nano biological composite regulating agent |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Jin et al. | Chitin and chitosan on the nanoscale | |
| Vasseghian et al. | Metal-organic framework-enabled pesticides are an emerging tool for sustainable cleaner production and environmental hazard reduction | |
| Jin et al. | Antimicrobial activity of zinc oxide nano/microparticles and their combinations against pathogenic microorganisms for biomedical applications: From physicochemical characteristics to pharmacological aspects | |
| Anand et al. | Graphene oxide and carbon dots as broad-spectrum antimicrobial agents–a minireview | |
| Pandit et al. | Graphene‐based antimicrobial biomedical surfaces | |
| Song et al. | Dispersible silica nanoparticles as carrier for enhanced bioactivity of chlorfenapyr | |
| US20190281833A1 (en) | Functionalized environmentally benign nanoparticles | |
| Durán et al. | Potential use of silver nanoparticles on pathogenic bacteria, their toxicity and possible mechanisms of action | |
| Zhang et al. | Bio-interface engineering of MXene nanosheets with immobilized lysozyme for light-enhanced enzymatic inactivation of methicillin-resistant Staphylococcus aureus | |
| Dong et al. | User-safe and efficient chitosan-gated porous carbon nanopesticides and nanoherbicides | |
| US20090028947A1 (en) | Using of nanosilver in poultry, livestock and aquatics industry | |
| AU2015376853B2 (en) | Improved pathogen inhibitor | |
| Giannousi et al. | Copper-based nanoparticles as antimicrobials | |
| CN102450269A (en) | Triazolone nanometer colloid suspending agent and preparation method thereof | |
| Barkat et al. | Silver nanoparticles and their antimicrobial applications | |
| Zong et al. | Redox and near-infrared light-responsive nanoplatform for enhanced pesticide delivery and pest control in rice: construction, efficacy, and potential mechanisms | |
| Caracciolo et al. | Recent progress and trends in the development of electrospun and 3D printed polymeric-based materials to overcome antimicrobial resistance (AMR) | |
| Lin et al. | Development of lignin-coated natural polysaccharide-based nanopesticides for both high foliar adhesion and rapid release of pesticide molecules against bacteriostasis | |
| Sowmya et al. | A critical review on nano-selenium based materials: synthesis, biomedicine applications and biocompatibility assessment | |
| Zhang et al. | In-situ coordination assembly of polyphenol with cage-like Prussian blue as an ecofriendly nanocarrier for site-specific pesticide delivery and sustained pest control | |
| Hu et al. | MOF-based stimuli-responsive controlled release nanopesticide: mini review | |
| Hassan | Antimicrobial Surface Coatings Containing Silver Nanoparticles. | |
| Li et al. | Synthesis and Characterization of Mesoporous Silica Nanoparticles Loaded with P-Cymene against Rice Bacterial Blight | |
| Roy et al. | Silver‐Based Nanoparticles for Antibacterial Activity: Recent Development and Mechanistic Approaches | |
| Kodaparthi et al. | Bactericidal Effects: Microbial Nanoparticles as Next-Generation Antimicrobials |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20120516 |