CN102445558A - Multi-piston trace reagent charging device - Google Patents
Multi-piston trace reagent charging device Download PDFInfo
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- CN102445558A CN102445558A CN2011104245413A CN201110424541A CN102445558A CN 102445558 A CN102445558 A CN 102445558A CN 2011104245413 A CN2011104245413 A CN 2011104245413A CN 201110424541 A CN201110424541 A CN 201110424541A CN 102445558 A CN102445558 A CN 102445558A
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- piston
- reagent
- reaction cup
- trace
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Abstract
The invention relates to the field of experimental apparatuses and specifically to a multi-piston trace reagent charging device. The device comprises a reaction cup, a support sleeved around the interior upper part of the reaction cup, a piston A, a piston B and a piston C respectively sleeved at the bottom, the middle part and the top in the support, and a push rod; the lower part of the piston A is preloaded with capillary tubes, the reaction cup is preloaded with a reagent A, a reagent B is preloaded between the piston A and the piston B, a reagent C is preloaded between the piston B and the piston C, a positioning snap ring is provided in the lower part of the reaction cup, and interior space of the positioning snap ring forms a reserved space for the piston A and a capillary tube assembly. The multi-piston trace reagent charging device provided in the invention can conveniently accomplish acquisition of a trace sample, accurate and quantitative charging of reagents and a variety of detection functions needed in nephelometry, transmission turbidimetry, biochemical colorimetry, immunofluorescence, ATP fluorescence, chemiluminescence immunoassay and the like in the fields of clinical diagnosis, laboratories and food safety monitoring in a short time.
Description
Technical field
The present invention relates to test the utensil field, particularly a kind of many pistons trace reagent charging point.
Background technology
Detect in enzyme linked immunosorbent detection, chemiluminescence immunoassay, fluorescence immunoassay detects, ATP enzyme fast detecting and biochemical and often need accomplish the sampling of micro-example, the filling of accurate quantification reagent and the operations such as use of serial pipettor tool than clinical diagnosis field, laboratory, food security monitoring field such as turbid, therefore needs special-purpose laboratory and a lot of corresponding labwares.At present; Accomplish detecting required all ingredients all is kept at independently among bottle or the bucket; And a subsidiary IC-card and instructions; The liquid transmission pipeline that needs complicacy on the detecting instrument is used to accomplish processes such as the filling of various liquid reagents, mixing, and need calculate test data through reading IC-card.Reacted waste liquid also need be discharged in the waste liquid barrel of instrument.Because the sample pin needs to use repeatedly, this has just brought more cross pollution, and it is bigger to detect error.Numerous liquid transmission pipelines of instrument internal make the detecting instrument complex structure, and cost is higher.Simultaneously, because that the IC-card in the kit be this kit is special-purpose, but in the daily use, the IC-card of different kits appears in conventional kit back warp regular meeting, thereby causes using with, causes the experimental result existence than mistake or mistake.
Summary of the invention
The technical matters that the present invention will solve provides the combination of one kind of multiple experiment utensils, the sampling that makes micro-example, the filling of accurate quantification reagent and testing process is easy and simple to handle, error is less, experimental result is reliable.
For solving the problems of the technologies described above, the technical scheme that the present invention taked is:
A kind of many pistons trace reagent charging point; Comprise reaction cup, be set in the reaction cup internal upper part support, be set with the piston A in the support inner bottom part, the piston B at middle part, the piston C and the push rod at top; Be provided with the location snap ring inside pre-installing reagent C, reaction cup bottom between prepackage reagent B, piston B and the piston C between prepackage reagent A, piston A and the piston B in piston A bottom prepackage kapillary, the reaction cup, snap ring inner space, location constitutes piston A and capillary module headspace.
Further, between said piston C and the push rod buffering sponge is housed.
Further, said push rod and support are for being slidingly matched or screw fit.
Further, a side of said reaction cup outside surface is provided with bar code and pastes the district, and opposite side is provided with frosted written indicia district.
Further, ears 9 are established in said reaction cup port.
Further, said support 6 outside surfaces are provided with the tooth form ring texture.
Adopt the beneficial effect that technique scheme produced to be: the present invention well gathers together reaction cup, kapillary, reagent A, reagent B, reagent C, can accomplish micro-example collection, the filling of accurate quantification reagent and various measuring ability in clinical diagnosis, laboratory, the food security monitoring fields such as scattering turbidimetry, turbid, the biochemical colorimetric of transmittance, immunofluorescence, ATP fluorescence and chemiluminescence immunoassay reaction detection at short notice easily.
Description of drawings
Fig. 1 is the cross-sectional view of original state of the present invention;
Fig. 2 is push rod of the present invention cross-sectional view when depressing;
Fig. 3 is a front elevation of the present invention.
Among the figure: 1, reaction cup; 2, kapillary; 3, piston A; 4, piston B;
5, piston C; 6, support; 7, push rod; 8, buffering sponge;
9, ears; 10, reagent C; 11, reagent B; 12, reagent A;
13, headspace; 14, bar code is pasted the district; 15, written indicia district;
16, location snap ring.
Embodiment
Below in conjunction with accompanying drawing and embodiment the present invention is done further detailed explanation.
Embodiment, see Fig. 1, Fig. 2 and shown in Figure 3:
A kind of many pistons trace reagent of the present invention charging point; Comprise reaction cup 1, support 6, piston A3, piston B4, piston C5, buffering sponge 8, push rod 7; Piston A3 bottom is pre-installed in kapillary 2, the reaction cup 1 and is provided with location snap ring 16 inside prepackage reagent C 10, reaction cup 1 bottom between prepackage reagent B11, piston B4 and the piston C5 between prepackage reagent A 12, piston A3 and the piston B4, and snap ring 16 inner spaces, location constitute the headspace 13 of piston A3 and kapillary 2 assemblies.Said push rod 7 support 6 endoporus of packing into, push rod 7 adopt with support 6 and are slidingly matched, but also the periwinkle collecting line cooperates.Said push rod 7 tops are adjacent to buffering sponge 8 upper surfaces, and piston C5 upper surface is adjacent to buffering sponge 8 lower surfaces.To between 20 microlitres, the charging capacity of said reagent A 12 is between 250 microlitres to 2 milliliter at 2 microlitres for said kapillary 2 capacity, and the charging capacity of said reagent B11 is between 50 microlitres to 1 milliliter, and the charging capacity of said reagent C 10 is between 50 microlitres to 1 milliliter.Said number of pistons, reagent type and quantity can be carried out suitable adjustment according to the concrete needs that use.
Said reaction cup 1 port is provided with ears 9 to increase the finger resistance, is convenient to promote push rod.
One side of said reaction cup 1 outside surface is provided with bar code and pastes district 14, pastes the two-dimensional bar that records the reagent parameter; Opposite side is provided with frosted written indicia district 15, so that write coded text.
Said support 6 outside surfaces are provided with the tooth form ring texture, make it and the reaction cup 1 inwall composition enclosure space of combining closely.
During use, from said reaction cup 1, extract support 6 assemblies (comprise support 6 and be set in its inner member), inhale the residual liquid in the unhairing tubule 2, and gather sample with kapillary 2 with thieving paper.Turn back to said support 6 assemblies in the reaction cup 1 then, the present invention 2～3 times that rocks from side to side fully is diluted in the reagent A 12 sample in the kapillary 2.Said push rod 7 is pushed in the support 6 fully, and this moment, sampling component piston A3 and kapillary 2 fell into the headspace 13 of reaction cup 1.The reagent C of pre-installing between the reagent B11 that pre-installs between said piston A3 and piston B4, piston B4 and piston C5 10 gets into reaction cup 1 simultaneously and mixes with reagent A 12.Because said piston B4 proportion is lighter than reagent, piston B4 floats on the liquid reagent surface in the reaction cup 1.Said piston C5 can not slip in the reaction cup 1 owing to combine closely to rest on support 6 lower ends with push rod 7 and buffering sponge 8.The present invention 2～3 times that rocks from side to side once more makes the abundant mixing of all reagent in the said reaction cup 1.Insert the present invention in the testing tool (HP083 series and
714 serial automated immunoassay analyzers of supporting use standing grain cypress biotechnology incorporated company) then; Testing tool at first reads the reagent parameter on the two-dimensional bar code that sticks in the said bar code stickup district 14, controls whole application of sample, testing process and calculates test result according to the reagent parameter information then.
The above only is a preferred embodiments of the present invention, so all equivalences of doing according to the described design of patent claim of the present invention, structure, characteristic and principle change or modify, includes in patent claim of the present invention.
Claims (8)
1. the charging point of piston trace reagent more than a kind; Comprise reaction cup (1), be set in the piston C (5) and the push rod (7) at the support (6) of reaction cup (1) internal upper part, the piston B (4) that is set with piston A (3) in support (6) inner bottom part, middle part, top; It is characterized in that: said piston A (3) bottom prepackage kapillary (2); Prepackage reagent A (12) in the said reaction cup (1); Prepackage reagent B (11) between said piston A (3) and the piston B (4); Pre-install reagent C (10) between said piston B (4) and the piston C (5), be provided with location snap ring (16) inside said reaction cup (1) inner bottom part, constitute the headspace (13) of said piston A (3) and kapillary (2) assembly in the location snap ring (16).
2. many pistons trace reagent charging point according to claim 1 is characterized in that: buffering sponge (8) is housed between said piston C (5) and the push rod (7).
3. many pistons trace reagent charging point according to claim 1 is characterized in that: said push rod (7) and support (6) are for being slidingly matched.
4. many pistons trace reagent charging point according to claim 1 is characterized in that: said push rod (7) is a screw fit with support (6).
5. many pistons trace reagent charging point according to claim 1 is characterized in that: a side of said reaction cup (1) outside surface is provided with bar code and pastes district (14), and opposite side is provided with frosted written indicia district (15).
6. many pistons trace reagent charging point according to claim 1 is characterized in that: ears (9) are established in said reaction cup (1) port.
7. many pistons trace reagent charging point according to claim 1 is characterized in that: said support (6) outside surface is provided with the tooth form ring texture.
8. many pistons trace reagent charging point according to claim 1; It is characterized in that: said kapillary (2) capacity at 2 microlitres between 20 microlitres; The charging capacity of said reagent A (12) is between 250 microlitres to 2 milliliter; The charging capacity of said reagent B (11) is between 50 microlitres to 1 milliliter, and the charging capacity of said reagent C (10) is between 50 microlitres to 1 milliliter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104245413A CN102445558A (en) | 2011-12-16 | 2011-12-16 | Multi-piston trace reagent charging device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104245413A CN102445558A (en) | 2011-12-16 | 2011-12-16 | Multi-piston trace reagent charging device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102445558A true CN102445558A (en) | 2012-05-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011104245413A Pending CN102445558A (en) | 2011-12-16 | 2011-12-16 | Multi-piston trace reagent charging device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107817358A (en) * | 2017-12-14 | 2018-03-20 | 江苏宝卫士生物科技有限公司 | A kind of diagnostic reagent detection means and its method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6821484B1 (en) * | 1998-09-02 | 2004-11-23 | Accip Biotech Aps | Apparatus for isolation of particles, preferably cell clusters |
| CN201196652Y (en) * | 2008-05-16 | 2009-02-18 | 厦门大学 | Non-valve injection type sample adding component |
| CN201421413Y (en) * | 2009-06-05 | 2010-03-10 | 何峰 | Liquid sample processing device |
| WO2011047902A1 (en) * | 2009-10-22 | 2011-04-28 | Gerhard Bonecker | Test set for a photometric measuring device and photometric measuring method for a sample liquid |
| CN102072949A (en) * | 2010-11-11 | 2011-05-25 | 郝书顺 | Integrated quantitative sampling and reagent adding device |
| CN202939178U (en) * | 2011-12-16 | 2013-05-15 | 苏州生水医学科技有限公司 | Multi-piston trace reagent charging device |
-
2011
- 2011-12-16 CN CN2011104245413A patent/CN102445558A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6821484B1 (en) * | 1998-09-02 | 2004-11-23 | Accip Biotech Aps | Apparatus for isolation of particles, preferably cell clusters |
| CN201196652Y (en) * | 2008-05-16 | 2009-02-18 | 厦门大学 | Non-valve injection type sample adding component |
| CN201421413Y (en) * | 2009-06-05 | 2010-03-10 | 何峰 | Liquid sample processing device |
| WO2011047902A1 (en) * | 2009-10-22 | 2011-04-28 | Gerhard Bonecker | Test set for a photometric measuring device and photometric measuring method for a sample liquid |
| CN102072949A (en) * | 2010-11-11 | 2011-05-25 | 郝书顺 | Integrated quantitative sampling and reagent adding device |
| CN202939178U (en) * | 2011-12-16 | 2013-05-15 | 苏州生水医学科技有限公司 | Multi-piston trace reagent charging device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107817358A (en) * | 2017-12-14 | 2018-03-20 | 江苏宝卫士生物科技有限公司 | A kind of diagnostic reagent detection means and its method |
| CN107817358B (en) * | 2017-12-14 | 2023-11-07 | 江苏宝卫士生物科技有限公司 | Diagnostic reagent detection device and method thereof |
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Application publication date: 20120509 |