CN102435657A - Ion-selective electrode flow injection analyser - Google Patents
Ion-selective electrode flow injection analyser Download PDFInfo
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- CN102435657A CN102435657A CN2011102857843A CN201110285784A CN102435657A CN 102435657 A CN102435657 A CN 102435657A CN 2011102857843 A CN2011102857843 A CN 2011102857843A CN 201110285784 A CN201110285784 A CN 201110285784A CN 102435657 A CN102435657 A CN 102435657A
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- peristaltic pump
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Abstract
The invention discloses an ion-selective electrode flow injection analyser. Ports of a three-way valve are connected with an ion selective electrode (ISE), a No.1 channel of a six-way valve and a peristaltic pump; the other end of the peristaltic pump A is connected with a total ionic strength adjustment buffer (TISAB) bottle; two ends of a peristaltic pump B are connected with a carrier liquid bottle and a No.6 channel; two ends of a peristaltic pump C are connected with a waste liquor bottle and a No.4 channel; a sample liquid bottle is connected with a No.3 channel; two ends of a separation column are connected with a No.2 channel and a No.5 channel; the bottom end of the ISE is provided with an ISE electrode membrane, a reflux tube is arranged below the ISE electrode membrane, and a gap between the reflux tube and the ISE electrode membrane is 0.5mm; a reference electrode and the ISE electrode are connected through a gravity separator consisting of an A-B channel and a C-D channel; inclination angles of the A-B channel and the C-D channel are 35 degrees and 45 degrees; and channels on two sides of the reflux tube are connected with the ends B and D of the gravity separator. The ion-selective electrode flow injection analyser has the advantages that: experimental reagents are saved, a detection line can be reduced, and a phenomenon that the detection limit is improved due to the dissolution of a salt type ISE membrane is inhibited.
Description
Technical field
The present invention relates to a kind of ISE flow injection analyzer, belong to the electrochemical analytical instrument technical field.
Background technology
(Flow Injection Analysis FIA) is a kind of new continuous Flow Analysis Technique of Denmark chemist proposition in 1974 to Flow Injection Analysis.This technology is to be injected into one to the sample solution of certain volume flowing; In the non-air reagent solution current-carrying at interval; The sample solution that is injected into flows into the reaction coil pipe; Form a zone, and with current-carrying in reagent mix, reaction, enter into flow-through detector again and carry out determination and analysis and record.At present, measure ISE (ISE) analysis and adopt the steady-state analysis method, reagent dosage is big, and analysis time is long, receives such environmental effects big, and can not be used for continuous analysis.
Summary of the invention
The technical matters that the present invention will solve is to provide a kind of ISE flow injection analyzer.It is few to use this instrument to have a reagent dosage, and analysis time is short, does not receive such environmental effects, can be used in the advantage of continuous analysis.
The present invention solves the technical scheme that its technical matters takes: this ISE flow injection analyzer is made up of sample collecting system, ISE electrode system and Computer I SE software analysis system.
In the sample collecting system, in three peristaltic pumps microtubular is housed respectively, three ports of T-valve are fixed with the screw that microtubular is housed respectively, and its left port is connected with the ISE electrode, and port is connected with 1 ﹟ passage of six-way valve, and right output port is connected with peristaltic pump A; The other end of peristaltic pump B is connected with TISAB liquid bottle through conduit; The end of peristaltic pump B is connected with the carrier fluid bottle through conduit, and the other end links to each other with 6 ﹟ passages of six-way valve, and the end of peristaltic pump C is connected with waste liquid bottle through conduit, and the other end links to each other with 4 ﹟ passages of six-way valve; Appearance liquid bottle links to each other with 3 ﹟ passages of six-way valve through conduit; Separating column is positioned at the upper end of six-way valve, and its two ends are fixed with screw, and is connected with 5 ﹟ passages with 2 ﹟ passages of six-way valve respectively; In the ISE electrode system, contrast electrode is fixed on the arc-spark stand with jackscrew, and left end is fixed with the screw that has microtubular, is connected with waste liquid bottle through conduit; The ISE electrode is fixed on the arc-spark stand with jackscrew; The ISE electrode film is equipped with in the bottom of ISE electrode; Be anti-stream pipe below; For well, contrast electrode is connected with the gravity separator that the ISE electrode is formed through A-B passage and C-D passage intermediate gaps with 0.3～0.9mm, wherein the range of tilt angles of A-B passage and C-D passage respectively with 25o～40o and 35o～50o for well; The passage of anti-stream pipe both sides links to each other with the D end with the B of gravity separator; Contrast electrode links to each other with the computing machine that has the ISE analysis software through the analytical instrument signaling interface with the ISE electrode.
The invention has the beneficial effects as follows: saved experiment reagent, using amount of reagent is 1% of gram method.Can reduce the phenomenon that detectability that detection line and the dissolving that suppresses because of salt ISE film cause improves, and not receive Effect of Environmental.TISAB content through threeway is flowed out is fixed, so base flow is stable.Separating column is installed on the sampling ring, is used to get rid of concentrating of interfering ion and measured component, reach the purpose that reduces detectability.The slit of 0.5mm is arranged between anti-stream pipe and the ISE film, and dead volume is little, and solution from device, do not have bubble at the salt bridge end of contrast electrode, so current potential is stablized through the gravity gas-liquid.Contrast electrode is after the ISE electrode, and two flow state that is in, so the chlorion that flows out of salt bridge end interfere ISE electrode not.Replaceable various ISE electrode.
Description of drawings
Below in conjunction with accompanying drawing and embodiment the present invention is further specified.
Fig. 1 is a structural representation of the present invention.
Label among the figure:
1, peristaltic pump A 2, peristaltic pump B 3, peristaltic pump C 4, T-valve 5, six-way valve
6, control power supply 7, microtubular 8, ISE electrode 9, conduit 10, TISAB liquid bottle
11, carrier fluid bottle 12, waste liquid bottle 13, appearance liquid bottle 14, separating column 15, contrast electrode
16, jackscrew 17, arc-spark stand 18, ISE electrode film 19, anti-stream pipe
20, A-B passage 21, C-D passage 22, analytical instrument signaling interface 23, computing machine.
Embodiment
Example 1
As shown in Figure 1, this ISE flow injection analyzer is made up of sample collecting system, ISE electrode system and Computer I SE software analysis system.The sample collecting system comprises three peristaltic pumps 1,2,3, and six-way valve 4 is formed with separating column 14, and the ISE electrode system comprises contrast electrode 15, arc-spark stand 17, ISE electrode 8, anti-stream pipe 19 and gravity gas-liquid separator.
In the sample collecting system; Three peristaltic pumps 1,2,3 are connected with control power supply 6 respectively; Microtubular 7 is housed respectively in each peristaltic pump, and three ports of T-valve 4 are fixed with the screw that microtubular 7 is housed respectively, and its left port is connected with ISE electrode 8; Port is connected with 1 ﹟ passage of six-way valve 5, and right output port is connected with peristaltic pump A1; The other end of peristaltic pump A1 is connected with TISAB liquid bottle 10 through conduit 9.The end of peristaltic pump B2 is connected with carrier fluid bottle 11 through conduit 9, and the other end links to each other with 6 ﹟ passages of six-way valve 5, and the end of peristaltic pump C3 is connected with waste liquid bottle 12 through conduit 9, and the other end links to each other with 4 ﹟ passages of six-way valve 5.Appearance liquid bottle 13 links to each other with 3 ﹟ passages of six-way valve 5 through conduit 9.Separating column 14 is positioned at the upper end of six-way valve 5, and its two ends are fixed with screw, and is connected with 5 ﹟ passages with 2 ﹟ passages of six-way valve 5 respectively.
Example 2
In the ISE electrode system, contrast electrode 15 usefulness jackscrews 16 are fixed on the arc-spark stand 17, and left end is fixed with the screw that has microtubular 7, is connected with waste liquid bottle 12 through conduit 9.ISE electrode 8 usefulness jackscrews 16 are fixed on the arc-spark stand 17; ISE electrode film 18 is equipped with in the bottom of ISE electrode 8; Be anti-stream pipe 19 below; Intermediate gaps is 0.5mm, and contrast electrode 15 is connected with the gravity separator that ISE electrode 8 is formed through A-B passage 20 and C-D passage 21, and wherein the angle of inclination of A-B passage 20 and C-D passage 21 is respectively 35o and 45o.The passage of anti-stream pipe 19 both sides links to each other with the D end with the B of gravity separator.Contrast electrode 15 links to each other with the computing machine that has the ISE analysis software 23 through analytical instrument signaling interface 22 with ISE electrode 8.
TISAB through peristaltic pump A can be quantitative in the exit of threeway outflow, so base flow is stable.Carrier fluid through peristaltic pump B can be quantitative the entering six-way valve; Sample solution gets into six-way valve through valve; Sample and carrier fluid mix the back and get into separating column in six-way valve, this both can get rid of interfering ion, can also carry out concentrating of measured component, reach the purpose that reduces detectability.Detect liquid through peristaltic pump A, through threeway, arrival is also impacted the ISE film, the slit of 0.5mm is arranged between anti-stream pipe and the ISE film, so dead volume is little, and the anti-gravity gas-liquid that flow to AB passage and CD passage composition of solution is from device.Disturb gas and a part of liquid of contrast electrode to flow out from the AB passage, neat liquid flows out from the CD passage, do not have bubble at the salt bridge end of contrast electrode, so current potential is stable.Contrast electrode is after the ISE electrode, and two flow state that is in, so the chlorion that flows out of salt bridge end interfere ISE electrode not.
Claims (1)
1. ISE flow injection analyzer, it is characterized in that: this analyser is made up of sample collecting system, ISE electrode system and Computer I SE software analysis system; In the sample collecting system; Microtubular (7) is housed respectively in three peristaltic pumps; Three ports of T-valve (4) are fixed with the screw that microtubular (7) is housed respectively; Its left port is connected with ISE electrode (8), and port is connected with 1 ﹟ passage of six-way valve (5), and right output port is connected with peristaltic pump A (1); The other end of peristaltic pump B (2) is connected with TISAB liquid bottle (10) through conduit (9); The end of peristaltic pump B (2) is connected with carrier fluid bottle (11) through conduit (9); The other end links to each other with 6 ﹟ passages of six-way valve (5); The end of peristaltic pump C (3) is connected with waste liquid bottle (12) through conduit (9), and the other end links to each other with 4 ﹟ passages of six-way valve (5); Appearance liquid bottle (13) links to each other with 3 ﹟ passages of six-way valve (5) through conduit (9); Separating column (14) is positioned at the upper end of six-way valve (5), and its two ends are fixed with screw, and is connected with 5 ﹟ passages with 2 ﹟ passages of six-way valve (5) respectively; In the ISE electrode system, contrast electrode (15) is fixed on the arc-spark stand (17) with jackscrew (16), and left end is fixed with the screw that has microtubular (7), is connected with waste liquid bottle (12) through conduit (9); ISE electrode (8) is fixed on the arc-spark stand (17) with jackscrew (16); ISE electrode film (18) is equipped with in the bottom of ISE electrode (8); Be anti-stream pipe (19) below; For well, contrast electrode (15) is connected with the gravity separator that ISE electrode (8) is formed through A-B passage (20) and C-D passage (21) intermediate gaps with 0.3～0.9mm, wherein the range of tilt angles of A-B passage (20) and C-D passage (21) respectively with 25o～40o and 35o～50o for well; The passage of anti-stream pipe (19) both sides links to each other with the D end with the B of gravity separator; Contrast electrode (15) links to each other with the computing machine that has the ISE analysis software (23) through analytical instrument signaling interface (22) with ISE electrode (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110285784 CN102435657B (en) | 2011-09-23 | 2011-09-23 | Ion-selective electrode flow injection analyser |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110285784 CN102435657B (en) | 2011-09-23 | 2011-09-23 | Ion-selective electrode flow injection analyser |
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| CN102435657A true CN102435657A (en) | 2012-05-02 |
| CN102435657B CN102435657B (en) | 2013-06-12 |
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| CN 201110285784 Expired - Fee Related CN102435657B (en) | 2011-09-23 | 2011-09-23 | Ion-selective electrode flow injection analyser |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107941890A (en) * | 2017-12-21 | 2018-04-20 | 南昌大学 | The automatic on-line monitoring method of chlorion in a kind of milk |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101603925A (en) * | 2009-07-23 | 2009-12-16 | 江苏德林环保技术有限公司 | The hyperchannel direction selecting flow-injection quick analysis system for hexavalent chromium water quality |
-
2011
- 2011-09-23 CN CN 201110285784 patent/CN102435657B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101603925A (en) * | 2009-07-23 | 2009-12-16 | 江苏德林环保技术有限公司 | The hyperchannel direction selecting flow-injection quick analysis system for hexavalent chromium water quality |
Non-Patent Citations (3)
| Title |
|---|
| EMÍLIA M. G. SANTOS等: "Determination of Gibberellic Acid by Sequential Injection Analysis Using a Potentiometric Detector Based on Mn(III)-Porphyrin with Improved Characteristics", 《J. BRAZ. CHEM. SOC.》 * |
| J. SLANINA等: "THE USE OF ION-SELECTIVE ELECTRODES IN MANUAL AND COMPUTER-CONTROLLED FLOW INJECTION SYSTEMS", 《ANALYTICA CHIMICA ACTA.》 * |
| WOLFGANG FRENZEL: "Enhanced Performance of Ion-selective Electrodes in Flow Injection Analysis: Non-Nernstian Response, Indirect Determination, Differential Detection and Modified Reverse Flow Injection Analysis", 《ANALYST》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107941890A (en) * | 2017-12-21 | 2018-04-20 | 南昌大学 | The automatic on-line monitoring method of chlorion in a kind of milk |
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| CN102435657B (en) | 2013-06-12 |
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