CN102433320B - Photosynthetic bacterium particle and preparation method thereof - Google Patents
Photosynthetic bacterium particle and preparation method thereof Download PDFInfo
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- CN102433320B CN102433320B CN 201110450339 CN201110450339A CN102433320B CN 102433320 B CN102433320 B CN 102433320B CN 201110450339 CN201110450339 CN 201110450339 CN 201110450339 A CN201110450339 A CN 201110450339A CN 102433320 B CN102433320 B CN 102433320B
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Abstract
The invention relates to a photosynthetic bacterium and a preparation method thereof, belongs to the technical field of environmental friendliness, and aims at solving the technical problems of high time consumption of conventional photosynthetic bacterium particle preparation process, low bacterium content of unit volume of the prepared photosynthetic bacterium particle, small divergence area after the particles are put into the water body, and low utilization rate of photosynthetic bacterium. The invention comprises a technical scheme of providing the photosynthetic bacterium, which consists of the following components in parts by weight: 450 to 500 parts of diatomite, 450 to 500 parts of zeolite powder, 30 to 50 parts of bentonite, and 1,000 parts of photosynthetic bacterium fluid.
Description
Technical field
The present invention relates to photosynthetic bacterium particle and preparation method thereof, belong to environmental technology field.
Background technology
To be a class carry out photosynthetic microorganism as the energy, the organism that can utilize occurring in nature under anaerobism illumination or aerobic dark condition, sulfide, ammonia etc. as the hydrogen donor carbon source of holding concurrently with light to photosynthetic bacterium (Photosynthetic bacteria, PSB).According to " the outstanding Bacteria Identification handbook of uncle (1974 the 8th edition) is classified the photosynthetic photosynthetic bacterium of non-oxygen-production as Bacteriophyta eubacterium guiding principle Rhodospirillales (Rhodospirillales), Bacteriophyta eubacterium guiding principle Rhodospirillales is divided into down again Rhodospirillineae (Rhodospirillineae) and green bacterium suborder (Chlorobiineae), be divided into Rhodospirillaceae (Rhodospirillaceae) and Chromatiaceae (Chlorobiaceae) under the Rhodospirillineae, be divided into green thiobacillus (Chlorobiaceae) and green thread bacillus (Chloroflexaceae) totally 18 genus under the green bacterium suborder, about 45 kinds.The photosynthetic bacterium toxic substances such as nitrite in the water body, sulfide of degrading can be restrained oneself the organic waste water of high density, the poisonous substances such as phenol cyanogen are had necessarily stand and capacity of decomposition, make it have the irreplaceable advantage of other microorganism aspect water treatment.But the thalline of photosynthetic bacterium is less, and the natural subsidence difficulty thalline occurs inevitably and runs off and two problems of solid-liquid separation in application.In order to solve this two problems, just need to constantly cultivate and add new fresh thalli, also need to carry out the work for the treatment of of solid-liquid separation, cause thus processing technological flow complicated, increase processing cost, had a strong impact on its applying aborning.For the better utilised photosynthetic bacterium, therefore produced the photosynthetic bacterium curing technology.The photosynthetic bacterium curing technology is by certain technique means photosynthetic bacterium to be fixed on the carrier, avoids thalline to run off, and improves the thalline utilization ratio, simplifies treatment process, improves water quality treatment efficient.The preparation photosynthetic bacterium particle is a kind of of photosynthetic bacterium curing technology.That uses at present has absorption method, crosslinking, an entrapping method.Wherein, absorption method is to rely on molecular force, hydrophobic force or ion bonding force between carrier and thalline that both are combined, and the method is simple to operate, and reaction conditions is gentle, does not affect cytoactive, is production application prospect the best way.Sorbing material commonly used mostly is inorganic or organic materials, such as: zeolite, porous ceramics, soft fiber etc.
The technology of preparing of present existing photosynthetic bacterium particle has: utilize the inorganic materials such as zeolite powder, slag to be combined with photosynthetic bacterium; Utilize the organic materialss such as sodium alginate, agar to be combined with photosynthetic bacterium; Utilize biomaterial such as active sludge and photosynthetic bacterium crosslinked.As: application number is 200610018362.9, and denomination of invention discloses the process for fixation of a kind of photosynthetic bacterium for the Chinese patent application of " process for fixation of photosynthetic bacterium ".It is 120～200 purpose zeolite powders that the method is selected granularity, be that to add viscosity be sodium alginate and the photosynthetic bacterium concentrated solution of 500～1000mpas for 1: 0.03～0.05: 0.2～0.3 ratio in mass ratio, mixed mixing thoroughly granulated, and placing concentration is the CaCl of 20～40g/L
2Harden in the solution, get finished product after the clear water rinsing.In this inventive method, the preparation process longer duration, water content is high, CaCl
2Large on the microbial activity impact, it is bonding not tight to prepare photosynthetic bacterium particle, and thalline runs off large in the settling process, the photosynthetic bacterium particle concentrating degree is inadequate, the contained photosynthetic bacterium comparatively small amt of unit volume, it is little to disperse area behind the input water body, not high to the utilization ratio of photosynthetic bacterium.
Summary of the invention
Main purpose of the present invention provides a kind of photosynthetic bacterium particle and preparation method.The technical problem that the present invention solves is existing photosynthetic bacterium particle preparation process length consuming time, and the unit volume bacteria containing amount of the photosynthetic bacterium particle of preparation is low, and it is little to disperse area behind the input water body, and the photosynthetic bacterium utilization ratio is low.
The invention provides a kind of photosynthetic bacterium particle, be grouped into by the one-tenth of following weight proportioning: 1000 parts of 450～500 parts in diatomite, 450～500 parts of zeolite powders, 30～50 parts of wilkinites and photosynthetic bacteria liquids.
Preferably, above-mentioned photosynthetic bacterium particle is grouped into by the one-tenth of following weight proportioning: 1000 parts of 475 parts in diatomite, 475 parts of zeolite powders, 30 parts of wilkinites and photosynthetic bacteria liquids.
Wherein, the preferred Rhodopseudomonas of above-mentioned photosynthetic bacterium (Rhodopseudomonas) photosynthetic bacterium.
Wherein, above-mentioned wilkinite is sodium bentonite.
Wherein, the concentration of above-mentioned photosynthetic bacteria liquid is 20～5,000,000,000/mL.
The present invention provides the preparation method of photosynthetic bacterium particle simultaneously, comprises the steps:
A, diatomite, zeolite powder are joined in the photosynthetic bacteria liquid, stir, filter, the preparation water content is at 50～60% photosynthetic bacterium concentrated solution;
B, diatomite, zeolite powder, wilkinite are joined in the photosynthetic bacterium concentrated solution that step a obtains, stir preparation photosynthetic bacterium material;
C, the photosynthetic bacterium material that step b is obtained carry out granulation, namely get photosynthetic bacterium particle.
Wherein, the weight proportion of above-mentioned diatomite, zeolite powder, wilkinite, photosynthetic bacteria liquid is: 475～500 parts: 475～500 parts: 30～50 parts: 1000 parts.
Wherein, above-mentioned diatomaceous using method is: get 25～50 parts of diatomite among the step a and join in the photosynthetic bacteria liquid, will remain diatomite and join in the photosynthetic bacterium concentrated solution in step b; The using method of above-mentioned zeolite powder is: get 25～50 parts of zeolite powders among the step a and join in the photosynthetic bacteria liquid, will remain zeolite powder and join in the photosynthetic bacterium concentrated solution in step b.
Wherein, among the step b water content of photosynthetic bacterium material 5～8%.
Wherein, among the step c by following specification granulation: every photosynthetic bacterium particle quality is 5g, and volume is 3cm
3
Photosynthetic bacterium particle storage endurance, quality guaranteed period reach 12 months among the present invention, convenient transportation, and sedimentation is rapid, can begin to disperse in 5 minutes after sinking to the bottom, and disperses 3～5 times of enlarged areas, forms the big area mycoderm.The raw material sources that the inventive method adopts are extensive, and production cost is low, do not add any chemical ingredients in the production, can not affect the activity of photosynthetic bacterium; With wilkinite as binding agent, because it also has adsorptivity, larger specific surface area absorption photosynthetic bacterium can be provided, so that 1000 kilograms of photosynthetic bacteria liquids can correspondingly be produced about 1000 kilograms of photosynthetic bacterium particles, the bacteria containing amount of the bacteria containing amount of every gram photosynthetic bacterium particle and every milliliter of bacterium liquid is suitable, has greatly improved the content of effective constituent in the photosynthetic bacterium particle.The rapid purifying contaminated water body of photosynthetic bacterium particle among the present invention, suppress other harmful algaes reach the growth of supporting bacterium (such as: blue-green algae etc.), reach and administer the effect that water pollutes.Find that after testing photosynthetic bacterium particle of the present invention can reduce rapidly chemical oxygen demand (COD) in the Sediments (COD) and ammonia nitrogen (NH
3-N) content raises bed mud dissolved oxygen amount (DO).The present invention can satisfy the needs of large-scale production, has wide market outlook.
Embodiment
The invention provides a kind of photosynthetic bacterium particle, be grouped into by the one-tenth of following weight proportioning: 1000 parts of 450～500 parts in diatomite, 450～500 parts of zeolite powders, 30～50 parts of wilkinites and photosynthetic bacteria liquids.
Preferably, above-mentioned photosynthetic bacterium particle is grouped into by the one-tenth of following weight proportioning: 1000 parts of 475 parts in diatomite, 475 parts of zeolite powders, 30 parts of wilkinites and photosynthetic bacteria liquids.
Wherein, the preferred Rhodopseudomonas of above-mentioned photosynthetic bacterium (Rhodopseudomonas) photosynthetic bacterium.
Wherein, above-mentioned wilkinite is sodium bentonite.
Wherein, the concentration of above-mentioned photosynthetic bacteria liquid is 20～5,000,000,000/mL.
The present invention provides the preparation method of photosynthetic bacterium particle simultaneously, comprises the steps:
A, diatomite, zeolite powder are joined in the photosynthetic bacteria liquid, stir, filter, the preparation water content is at 50～60% photosynthetic bacterium concentrated solution;
B, diatomite, zeolite powder, wilkinite are joined in the photosynthetic bacterium concentrated solution that step a obtains, stir preparation photosynthetic bacterium material;
C, the photosynthetic bacterium material that step b is obtained carry out granulation, namely get photosynthetic bacterium particle.
Wherein, the weight proportion of above-mentioned diatomite, zeolite powder, wilkinite, photosynthetic bacteria liquid is: 475～500 parts: 475～500 parts: 30～50 parts: 1000 parts.
Wherein, above-mentioned diatomaceous using method is: get 25～50 parts of diatomite among the step a and join in the photosynthetic bacteria liquid, will remain diatomite and join in the photosynthetic bacterium concentrated solution in step b; The using method of above-mentioned zeolite powder is: get 25～50 parts of zeolite powders among the step a and join in the photosynthetic bacteria liquid, will remain zeolite powder and join in the photosynthetic bacterium concentrated solution in step b.
Wherein, among the step b water content of photosynthetic bacterium material 5～8%.
Wherein, among the step c by following specification granulation: every photosynthetic bacterium particle quality is 5g, and volume is 3cm
3
Photosynthetic bacterium among the present invention (Photosynthetic bacteria) is called for short PSB, it is a bacterioid the most ancient on the earth, its thalline is nutritious, protein content is up to 65%, and is rich in biologically active substance and the trace elements such as multivitamin, coenzyme, strong adaptability, can restrain oneself the organic waste water of high density, have stronger decomposition and inversion ability, the poisonous substances such as phenol, cyanogen are had certain capacity of decomposition, aspect water treatment, have the irreplaceable advantage of other microorganism.Be applicable to photosynthetic bacterium of the present invention and can select the photosynthetic bacterium of Rhodospirillaceae (Rhodospirillaceae) Rhodopseudomonas (Rhodopseudomonas), for example Rhodopseudomonas palustris (Rhodopseudanonas palustris).In actual production, can be according to the suitable photosynthetic bacteriums of selection such as range of application of working condition, product.The inventor studies discovery, and photosynthetic bacteria liquid concentration reaches 20～5,000,000,000/mL and namely can be used for the present invention.
Wilkinite is as binding agent among the present invention.Wilkinite adsorbable 8～15 times to the water yield of own vol, volumetric expansion can reach several times to 30 times.Under many water conditions, the wilkinite crystalline structure is very fine, makes it have many good characteristics, such as polymolecularity, suspension, swelling, cohesiveness, adsorptivity, cationic exchange etc.Bentonitic main component is montmorillonite.Wilkinite not only can be used for the photosynthetic bacterium that bonds in the present invention, can also adsorb photosynthetic bacterium, the pollutent after photosynthetic bacterium particle is rendered to water body in all right adsorbed water body, complementary raising purifying water effect.According to bentonitic interlayer cation kind wilkinite is divided into four classes: interlayer cation is Na
+The time claim sodium bentonite; Interlayer cation is Ca
2+The time claim calcium-base bentonite; Interlayer cation claims the hydrogen base bentonite when being H+; Interlayer cation claims organobentonite when being organic cation.Wherein, sodium bentonite thing water-intake rate and expansion multiple are large, cation exchange capacity is high, in water medium good dispersity, higher plasticity-and stronger cohesiveness are arranged, have superior Physicochemical character and process technology performance.Use in the present invention sodium bentonite can make the result of use of photosynthetic bacterium particle better.
Diatomite and zeolite powder play sorbent material among the present invention.The characteristics such as that diatomite and zeolite powder all have is nontoxic, porousness, larger specific surface area, porosity are large, chemical stability, adsorptivity are strong, ion exchangeable, be widely used as sorbent material, ion-exchanger, also can be used for the aspects such as drying, purification and sewage disposal of gas.Therefore, diatomite and zeolite powder are very suitable for the absorption carrier as the preparation photosynthetic bacterium particle.Diatomite and zeolite powder are bought from market and are got final product among the present invention.Adopt in the present invention exquisite diatomite can make the result of use of the photosynthetic bacterium particle for preparing better.
Diatomite and zeolite powder use in two steps among the preparation method of the present invention, add first a part of zeolite powder and diatomite in photosynthetic bacteria liquid, be in order to allow zeolite powder and diatomite fully adsorb photosynthetic bacterium, then remove part moisture in the bacteria-removing liquid by filtration, obtain the photosynthetic bacterium concentrated solution; Adding residue diatomite, zeolite powder and wilkinite again in the photosynthetic bacterium concentrated solution, is to be used for granulation for the photosynthetic bacterium material that obtains boning good.In actual production, can join according to the condition adjustment such as aperture of the size of selected photosynthetic bacterium, filter plant diatomite in the photosynthetic bacteria liquid and the weight of zeolite powder.After a part of diatomite and zeolite powder joined photosynthetic bacteria liquid, want uniform stirring to make diatomite and zeolite powder fully adsorb photosynthetic bacterium, then after filtration thalline is adsorbed rear remaining moisture removal, can selects filter plant according to other the requirement of the size of selected photosynthetic bacterium and product during filtration.Good for the bonding that makes photosynthetic bacterium particle, can not affect again simultaneously photosynthetic bacterium particle and after input, disperse effect, can control the water content of photosynthetic bacterium material 5～8%.The standard of granulation is that every photosynthetic bacterium particle quality is 5g, and volume is 3cm
3, granulation can be carried out with modes such as machinery or craft, adopts mechanical system to enhance productivity.
For beneficial effect of the present invention is described, take application number as 200610018362.9, denomination of invention be " process for fixation of photosynthetic bacterium " Chinese patent application as a comparison, process for fixation according to the photosynthetic bacterium of this Patent Application Publication prepares photosynthetic bacterium particle, is used for making comparisons with the photosynthetic bacterium particle of preparation method's preparation of the present invention.
The present invention to settling time, the time opening of dispersing of the photosynthetic bacterium particle for preparing, disperse the concluding time, disperse area and measure.Settling time, the time opening of dispersing, disperse the concluding time all with the release time as time zero.Dispersing area compares according to specific surface area.
The present invention has also measured the detergent power of photosynthetic bacterium particle to Sediments.
Below in conjunction with embodiment the specific embodiment of the present invention is done wherein description.
Photosynthetic bacterium is selected Rhodopseudomonas palustris (Rhodopseudanonas palustris) among the following embodiment, belong to Rhodospirillaceae, Rhodopseudomonas, can be used for purification of water quality, sewage disposal, feed level microbe additive etc., its bacterial concentration is 3,000,000,000/mL.Wherein, bacterium source: Methane Scientific Research Inst., Ministry of Agriculture; Bacterial strain deposit number: 00309.
Use in the following embodiments sodium bentonite, its main component is the montmorillonite of 60～88% (wt/wt), expansion capacity is 25～50ml/g, colloid index 〉=99ml/15g, the 2h water-intake rate is 250～350%, moisture≤12%, green compression strength 〉=0.23 (Mpa), inhale blue amount 〉=80mmol/g, Na
2O 〉=1.28%.
Using in the following embodiments fineness is 200 purpose diatomite, its main component is the water of ferric oxide, 8～10% (wt/wt) of aluminium sesquioxide, 4% (wt/wt) of the silicon-dioxide, 15～18% (wt/wt) of 70% (wt/wt), and loose density is 0.40g/cm3.
Using in the following embodiments fineness is 200 purpose zeolite powders, and its main component is the magnesium oxide of calcium oxide, 2.38% (wt/wt) of ferric oxide, 2.71% (wt/wt) of aluminium sesquioxide, 1.35% (wt/wt) of the silicon-dioxide, 13.46% (wt/wt) of 62.87% (wt/wt).
0.001～0.1 micron the filter membrane that uses among the following embodiment is the PP hollow fiber ultrafiltration membrane.The PP hollow fiber ultrafiltration membrane is the asymmetric semipermeable membrane that utilizes macromolecular material to adopt special process to make.It is the capillary hollow shape, the tube wall micropore that gathers, the aperture of ultra-filtration membrane is greatly in 0.001～0.1 micrometer range, molecular weight cut-off is about 1000～500000 dalton, under the effect of pressure, stoste flows in film or outside the film, solvent wherein or small molecules can see through film, be collected as ultrafiltrated, and (the protein of macromolecular substance wherein, enzyme classes, nucleic acid, polysaccharide etc.) and colloidal particle (latex, micropartical), bacteriums etc. are trapped within outside the film, be recycled mobile stoste and take away and become concentrated solution, thereby realize the separation of material, concentrate and purification.
The preparation of embodiment 1 photosynthetic bacterium particle
A, 25 kilograms of diatomite and 25 kilograms of zeolite powders are added in 1000 kilograms of photosynthetic bacteria liquids, fully stir, make diatomite and zeolite powder fully adsorb photosynthetic bacterium, 0.001～0.1 micron PP hollow fiber ultrafiltration membrane is filtered, and gets water content at 50% photosynthetic bacterium concentrated solution;
B, the wilkinite of 425 kilograms of diatomite, 425 kilograms of zeolite powders and 30 kilograms is joined in the photosynthetic bacterium concentrated solution that step a obtains, stir, obtain water content at 8% photosynthetic bacterium material;
C, be 5g by every photosynthetic bacterium particle quality, volume is 3cm
3Specification, with the roller tablets press photosynthetic bacterium material that step b obtains is carried out granulation, namely get photosynthetic bacterium particle.
The settling time of the photosynthetic bacterium particle for preparing, the time opening of dispersing, disperse the concluding time, disperse area and see Table 1.
The preparation of embodiment 2 photosynthetic bacterium particles
A, 25 kilograms of diatomite and 25 kilograms of zeolite powders are added in 1000 kilograms of photosynthetic bacteria liquids, fully stir, make diatomite and zeolite powder fully adsorb photosynthetic bacterium, 0.001～0.1 micron PP hollow fiber ultrafiltration membrane is filtered, and gets water content at 50% photosynthetic bacterium concentrated solution;
B, the wilkinite of 450 kilograms of diatomite, 450 kilograms of zeolite powders and 30 kilograms is joined in the photosynthetic bacterium concentrated solution that step a obtains, stir, obtain water content at 7.4% photosynthetic bacterium material;
C, be 5g by every photosynthetic bacterium particle quality, volume is 3cm
3Specification, with Doubleroller compaction granulator the photosynthetic bacterium material that step b obtains is carried out granulation, namely get photosynthetic bacterium particle.
The settling time of the photosynthetic bacterium particle for preparing, the time opening of dispersing, disperse the concluding time, disperse area and see Table 1.
The preparation of embodiment 3 photosynthetic bacterium particles
A, 25 kilograms of diatomite and 25 kilograms of zeolite powders are added in 1000 kilograms of photosynthetic bacteria liquids, fully stir, make diatomite and zeolite powder fully adsorb photosynthetic bacterium, 0.001～0.1 micron PP hollow fiber ultrafiltration membrane is filtered, and gets water content at 50% photosynthetic bacterium concentrated solution;
B, the wilkinite of 475 kilograms of diatomite, 475 kilograms of zeolite powders and 50 kilograms is joined in the photosynthetic bacterium concentrated solution that step a obtains, stir, obtain water content at 5% photosynthetic bacterium material;
C, be 5g by every photosynthetic bacterium particle quality, volume is 3cm
3Specification, with Doubleroller compaction granulator the photosynthetic bacterium material that step b obtains is carried out granulation, namely get photosynthetic bacterium particle.
The settling time of the photosynthetic bacterium particle for preparing, the time opening of dispersing, disperse the concluding time, disperse area and see Table 1.
Comparative Examples
Take application number as 200610018362.9, denomination of invention prepares photosynthetic bacterium particle for the disclosed method of Chinese patent application of " process for fixation of photosynthetic bacterium ":
A, be that the calcium hydrogen phosphate solution of 5g/L is added in the photosynthetic bacteria culture solution with concentration, spend the night that make the thalline flocculation, siphonage sucks supernatant liquor, obtains the photosynthetic bacterium concentrated solution;
B, to get granularity be 140 purpose commodity zeolite powder 50kg, and adding viscosity is the sodium alginate 2.5kg of 800mpas, mixes thoroughly, adds photosynthetic bacterium concentrated solution 10kg again, and mixing is used the nodulizer granulation formation, and it is the CaCl of 30g/L that the particle after the moulding places concentration
2The 6h that hardens in the solution takes out with tap water rinsing twice after the sclerosis, namely gets photosynthetic bacterium particle.
The settling time of the photosynthetic bacterium particle for preparing, the time opening of dispersing, disperse the concluding time, disperse area and see Table 1.
The photosynthetic bacterium particle settling velocity for preparing in table 1 embodiment 1～3, the Comparative Examples and disperse area estimation
Settling time, the time opening of dispersing in the table 1, disperse the concluding time all with the release time as time zero, disperse area and compare according to specific surface area.Data can be found out from table 1: the photosynthetic bacterium particle in the Comparative Examples just begins to disperse in the sinking process because bonding dynamics is inadequate, and particle is more fluffy, and it is large to cause thalline to run off; Because being subjected to the cause of alginate calcium curing, it is little to disperse area again, and the photosynthetic bacterium bond area is little.The photosynthetic bacterium particle sedimentation is fast among the embodiment 1～3, bonding good, in settling process, do not disperse, can not cause thalline to run off, disperse 3～5 times of enlarged areas, can form large-area mycoderm.
The detergent power of the photosynthetic bacterium particle for preparing in embodiment 4 embodiment 1～3, the Comparative Examples is measured
The bed mud that extracts in certain pond compares experiment, and the contamination index of bed mud is as follows: pH is 6.89, and chemical oxygen demand (COD) (COD) is 78.5mg/L, and bed mud dissolved oxygen amount (DO) is 2.95mg/L, ammonia nitrogen (NH
3-N) content is 1.13mg/L.
Experiment grouping: totally 5 groups of photosynthetic bacterium particle, photosynthetic bacteria liquids in photosynthetic bacterium particle, the Comparative Examples among photosynthetic bacterium particle, the embodiment 3 among photosynthetic bacterium particle, the embodiment 2 among the embodiment 1.Get 5 parts of above-mentioned bed muds, each group uses 1 minute bed mud to compare experiment, the results are shown in Table 2.
Water-quality guideline measuring method: pH adopts pH meter (the portable PH meter of Shanghai thunder magnetic PHBJ-260) directly to measure; COD adopts potassium dichromate process to measure (with reference to GB/T 11914-1989); DO adopts portable dissolved oxygen analytic instrument (the portable dissolved oxygen meter of Shanghai thunder magnetic JPBJ-608 type) directly to measure; NH
3-N content adopts nessler reagent spectrophotometry (with reference to GB/T 7479-1987).
The detergent power of the photosynthetic bacterium particle for preparing in table 2 embodiment 1～3, the Comparative Examples is measured
As can be seen from Table 2: after the photosynthetic bacterium particle of the photosynthetic bacterium particle of embodiment 1～3, Comparative Examples and photosynthetic bacteria liquid are processed 7 days, the chemical oxygen demand (COD) of Sediments (COD) and ammonia nitrogen (NH
3-N) content has had reduction, and dissolved oxygen amount (DO) has had raising simultaneously, illustrates Sediments has been played cleaning action.Further analyze and find: at the 7th day that processes, after the photosynthetic bacterium particle of embodiment 1～3 is processed, the COD of Sediments has reduced respectively 46.36%, 57.83%, 51.71%, apparently higher than 32.73% of 24.95% and photosynthetic bacteria liquid of Comparative Examples; After the photosynthetic bacterium particle of embodiment 1～3 was processed, the DO of Sediments had increased respectively 46.44%, 82.03%, 68.13%, apparently higher than 38.98% of 31.53% and photosynthetic bacteria liquid of Comparative Examples; The NH of Sediments after the photosynthetic bacterium particle of embodiment 1～3 is processed
3-N content has reduced respectively 49.55%, 65.48%, 50.89%, be higher than Comparative Examples 27.43% and photosynthetic bacteria liquid 31.86%.In addition, data can be found out from table, and are especially ideal with the decontamination effect improving of photosynthetic bacterium particle among the embodiment 2.
Claims (7)
1. photosynthetic bacterium particle, it is characterized in that: the one-tenth by the following weight proportioning is grouped into: 1000 parts of 450～500 parts in diatomite, 450～500 parts of zeolite powders, 30 ~ 50 parts of wilkinites and photosynthetic bacteria liquids; Described photosynthetic bacterium is the Rhodopseudomonas photosynthetic bacterium; The concentration of described photosynthetic bacteria liquid is 20 ~ 5,000,000,000/mL.
2. photosynthetic bacterium particle according to claim 1, it is characterized in that: the one-tenth by the following weight proportioning is grouped into: 1000 parts of 475 parts in diatomite, 475 parts of wilkinites, 30 parts of wilkinites and photosynthetic bacteria liquids.
3. photosynthetic bacterium particle according to claim 1 and 2, it is characterized in that: described wilkinite is sodium bentonite.
4. the preparation method of photosynthetic bacterium particle comprises the steps:
A, diatomite, zeolite powder are joined in the photosynthetic bacteria liquid, stir, filter, the preparation water content is at 50 ~ 60% photosynthetic bacterium concentrated solution; Described photosynthetic bacterium is the Rhodopseudomonas photosynthetic bacterium; The concentration of described photosynthetic bacteria liquid is 20 ~ 5,000,000,000/mL;
B, diatomite, zeolite powder, wilkinite are joined in the photosynthetic bacterium concentrated solution that step a obtains, stir preparation photosynthetic bacterium material;
C, the photosynthetic bacterium material that step b is obtained carry out granulation, namely get photosynthetic bacterium particle;
The weight proportion of above-mentioned diatomite, zeolite powder, wilkinite, photosynthetic bacteria liquid is: 1000 parts of 475 ~ 500 Fen ︰, 475 ~ 500 Fen ︰, 30 ~ 50 Fen ︰.
5. the preparation method of photosynthetic bacterium particle according to claim 4, it is characterized in that: described diatomaceous using method is: get 25 ~ 50 parts of diatomite among the step a and join in the photosynthetic bacteria liquid, will remain diatomite and join in the photosynthetic bacterium concentrated solution in step b; The using method of described zeolite powder is: get 25 ~ 50 parts of zeolite powders among the step a and join in the photosynthetic bacteria liquid, will remain zeolite powder and join in the photosynthetic bacterium concentrated solution in step b.
6. the preparation method of photosynthetic bacterium particle according to claim 4, it is characterized in that: the water content of photosynthetic bacterium material is 5 ~ 8% among the step b.
7. the preparation method of photosynthetic bacterium particle according to claim 4 is characterized in that: among the step c by following specification granulation: every photosynthetic bacterium particle quality is 5g, and volume is 3cm
3
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