CN102430340A - Method for testing integrity of ultra-filtration membrane envelope - Google Patents
Method for testing integrity of ultra-filtration membrane envelope Download PDFInfo
- Publication number
- CN102430340A CN102430340A CN201110360538XA CN201110360538A CN102430340A CN 102430340 A CN102430340 A CN 102430340A CN 201110360538X A CN201110360538X A CN 201110360538XA CN 201110360538 A CN201110360538 A CN 201110360538A CN 102430340 A CN102430340 A CN 102430340A
- Authority
- CN
- China
- Prior art keywords
- film bag
- milipore filter
- embryo
- filter film
- antigen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for detecting the integrity of an ultra-filtration membrane envelope; the method comprises the following steps of: firstly, correctly installing a new membrane envelope according to a standard operation method, staring a machine, carrying out HA (hemagglutination) test on withdrawn penetrating fluid after the machine stably operates, if HA is larger than 3log<2>, indicating that the ultra-filtration membrane envelope is incomplete, ending the test; if HA is less than or equal to 3log<2>, and continuously testing; by selecting sensitive virus, inoculating chicken embryo specific to the effluent dialysate of the virus, cultivating the chicken embryo, then determining whether the virus exists in effluent dialysate by a biological detection method, and illustrating whether the ultra-filtration membrane envelope is complete. The method is simple and practical, can carry out on line test, is more accurate in comparison with factory methods and can be used for testing the single membrane envelope, multiple membrane envelopes and systems.
Description
Technical field
The present invention relates to a kind of integrality detection method of film bag, especially a kind of integrality detection method of milipore filter film bag.
Background technology
Membrane separation technique is according to drug activity and its molecular structure and the closely-related characteristic of molecular weight level, through the membrane module of selecting for use difference to hold back characteristic constitute film separation system to active ingredient separate, concentrate, purifying.Operation is motive force with pressure usually, can accomplish at normal temperatures, need not to add other material, has the advantage of high selectivity.
Film separates and comprises micro-filtration, and ultrafiltration and nanofiltration all are to be the process of motive force with the pressure difference, and its separating mechanism is that particulate sieves in the fenestra convection cell.Micro-filtration membrane be (MF) finger-hole footpath between 0.1-10um, between milipore filter (UF) aperture 0.01-0.1um, NF membrane (NF) aperture is generally between 0.001-0.05um.Usually the particle diameter of virus is generally between 0.01-0.08um, and bacterium liquid, virus have less relative molecular weight, therefore uses the milipore filter concentrated antigen, and is effective, and stability is strong, and the production that is suitable for certain scale is used.
The milipore filter screening process is a driving force with the pressure differential of film both sides, is filter medium with the milipore filter; Under certain pressure; When stoste flow through the film surface, the many tiny micropore that gathers in the milipore filter surface only allowed water and small-molecule substance through becoming through liquid, and volume then is trapped within the liquid feeding side of film in the stoste greater than the material in film surface micro aperture; Become concentrate, thereby realize purification, separation and concentrated purpose stoste.
When using milipore filter film bag that antigen is concentrated, complete film bag can permeate away hydrone, and enrichment antigen particulate reaches concentrated purpose.
Advantages such as the milipore filter isolation technics has that product yield is high, efficient is high, energy consumption is low, simple to operate, environmental friendliness; In traditional drug production, present significant technical advantage; But because the special macromolecular structure of film regenerates, cleans, utilizes frequency higher, film seepage phenomenon can appear.In fact, owing to the membrane module installation pipeline that constitutes film separation system is complicated, tie point is more, therefore is when appearance is unusual in the film course of work, and is relatively more difficult to the leakage detection work of piece-rate system.
The integrality of film bag is the prerequisite that guarantees separating effect, and the integrity detection of film bag fiber mostly depends on the detection method of film bag producer, and finds in the practical application that producer's detection method detects qualified film bag, and is still bad to viral separating effect.In fact be used for virus and separate, to having relatively high expectations of film bag, the breakage that membrane fiber is trickle can cause virus to reveal, thereby causes antigen losses and to the pollution of environment.
Film integrality detects at present, and the method that generally provides according to equipment producer is carried out the bubble point test method and detected.Open the source of the gas of integrity test device, system is pressurizeed.In system's pressure process, the air velocity that shows on the integrity test device at first will improve, and drop to a stable flow velocity then.The stable flow velocity of this that measures is exactly air forward streams (diffuse flow), if this stable flow velocity has been reduced to and can have accepted under the standard, and consistent with previous value, the film integrality test has been passed through in explanation so.
The concrete detection method that equipment producer provides is following:
Before membrane stack was carried out integrity detection, sufficient cleaning and flushing should be passed through by system, and residual cleaning agent can have very big influence to the result.Confirm that system is cleaned, after film is drenched fully, with intrasystem water emptying, the source of the gas of adjustable in pressure is connected on the import or the refluxing opening of membrane stack, will not connect the import or the refluxing opening sealing of source of the gas, it is open seeing through the liquid mouth.Slowly be pressurized to appointment air pressure, stablize then and let residual water discharge in 5 minutes.Measure and record air pressure, temperature and a gas flow from coming out through mouth; The agent of gas flow available gas flow is measured; The flow and the set quota that relatively detect, if milipore filter is imperfect, its measured air mass flow will substantially exceed the upper limit of flow velocity index in the table.
The report that also has is used the noise measuring method, but because the pressure and the flow velocity of tangential flow filtration are bigger, the method for noise measuring is inadvisable.
Summary of the invention
Whether damaged technical problem to be solved by this invention is, a kind of online test method is provided, can detect the integrality detection method of film bag milipore filter film bag.
In order to solve the problems of the technologies described above, the technical scheme that the present invention adopts is: a kind of integrality detection method of milipore filter film bag may further comprise the steps:
(1) at first new film bag is correctly installed according to the manufacturer's standard method of operating, is opened machine, treat that running is stable after, detect;
When (2) detecting; The antigen liquid that will contain the antigen that separates with needs as the NDV that indicates antigen is got film bag diffusate through milipore filter film bag, the penetrating fluid that takes out is HA detects (hemagglutination test (HA test)); Survey blood clotting valency HA; If HA＞3log2 explains that milipore filter film bag is imperfect, detect and finish; If HA≤3log2 proceeds following step (3) and detects;
(3) get 10 of the chicken embryos that ten ages in days do not have antibodies specific, wherein inoculation film bag diffusate 0.2ml in 8 every embryo allantois of embryo stay 2 pieces to compare; Inoculating back 37 ℃ cultivated 120 hours; Observe 2 time, if embryo non-specific death be no more than 1 according to the chicken embryo every day; Find that during this time dead embryo places 2-8 ℃ of preservation at once, carry out following step (4) after 120 hours and detect;
(4) all chicken embryos are got blastochyle and measure blood clotting valency HA, if HA≤3log2 explains the complete of milipore filter film bag, if HA＞3log2 explains the imperfect of milipore filter film bag.
The NDV antigen liquid is counted by volume and is accounted for 0.5-1% in the said antigen liquid.
The said antigen that needs to separate is avian influenza virus or newcastle disease virus.
The invention has the beneficial effects as follows: utilize the sensitiveness of virus, and virus is to erythrocytic agglutination, through detecting agglutination titer; Judge the integrality of film bag, method is simple, and the method for comparing producer is more accurate; Can be used for the detection of single film bag, the detection of a plurality of film bags and system.With the integrality of the viral detection film of tiring, detection method is simple and easy to do, and need not stop to produce.
The specific embodiment
Below in conjunction with the specific embodiment the present invention is done further explain:
The integrality detection method of milipore filter film bag of the present invention may further comprise the steps:
(1) at first new film bag is correctly installed according to the manufacturer's standard method of operating, is opened machine, treat that running is stable after, detect;
When (2) detecting; The antigen liquid that will contain the antigen that separates with needs as the NDV that indicates antigen is got film bag diffusate through milipore filter film bag, the penetrating fluid that takes out is HA detects (hemagglutination test (HA test)); Survey blood clotting valency HA; If HA＞3log2 explains that milipore filter film bag is imperfect, detect and finish; If HA≤3log2 proceeds following step (3) and detects;
(3) get 10 of the chicken embryos that ten ages in days do not have antibodies specific, wherein inoculation film bag diffusate 0.2ml in 8 every embryo allantois of embryo stay 2 pieces to compare; Inoculating back 37 ℃ cultivated 120 hours; Observe 2 time, if embryo non-specific death be no more than 1 according to the chicken embryo every day; Find that during this time dead embryo places 2-8 ℃ of preservation at once, carry out following step (4) after 120 hours and detect;
(4) all chicken embryos are got blastochyle and measure blood clotting valency HA, if HA≤3log2 explains the complete of milipore filter film bag, if HA＞3log2 explains the imperfect of milipore filter film bag.
The NDV antigen liquid is counted by volume and is accounted for 0.5-1% in the said antigen liquid.
The said antigen that needs to separate is avian influenza virus or newcastle disease virus.
Newcastle disease virus virus (NDV) belongs to Paramyxoviridae, and paramyxovirus genus, nucleic acid are single stranded RNA.Ripe virion is spherical in shape, and diameter is 120-300nm.By the spirality symmetrical disk around nucleocapsid and cyst membrane form.The cyst membrane surface has the fibre of radial arrangement prominent, wherein contains neuraminidase, and therefore virus can be adsorbed on the red blood cell surface of bird, causes erythrocytic aggegation.The median infective dose EID of NDV
50: every 0.1ml viral level>=10
8EID
50The particle diameter of newcastle disease virus is less, according to blood coagulation property is arranged, still has sensitive blood coagulation phenomenon when diluting higher titre, because detection method is simple, can be used as sensitive indicator simultaneously.
And during to the antigen assay with through hemagglutination test (HA test), it is main detecting that HA tires.And measure easy, quick, easy row, be convenient to aborning carry out.
Following mask body describes:
Measure the method for blood clotting valency HA, claim the hemagglutination test (HA test) method again, see " People's Republic of China's veterinary biologics rules " version hemagglutination test (HA test) in 2000 for details.
The film bag that adopts is purchased in your company quite, the molecular cut off 100kd of film bag, and this ultrafiltration membrane stack, molecular cut off 100,000 molecular weight, effective filtration area are 0.5m
2, pressure 0.6MPa.
In addition, can correctly install according to standard operating instructions, open machine new film bag, treat that running is stable after, take off the stream diffusate, survey blood clotting valency HA respectively, as the reference point of integrality.Percentage among the following embodiment is percent by volume.
Embodiment 1
(1) at first new film bag is correctly installed according to the manufacturer's standard method of operating, is opened machine, treat that running is stable after, detect;
When (2) detecting; The antigen liquid that will contain 0.5% avian influenza virus that separates with 99.5% needs as the NDV that indicates antigen is got film bag diffusate 10ml through milipore filter film bag, and the penetrating fluid of taking-up is done HA detection (hemagglutination test (HA test)); Survey blood clotting valency HA; If HA＞3log2 explains that milipore filter film bag is imperfect, detect and finish; If HA≤3log2 proceeds following step (3) and detects;
(3) get 10 of the chicken embryos that ten ages in days do not have antibodies specific, wherein inoculation film bag diffusate 0.2ml in 8 every embryo allantois of embryo stay 2 pieces to compare; Inoculating back 37 ℃ cultivated 120 hours; Observe 2 time, if embryo non-specific death be no more than 1 according to the chicken embryo every day; Find that during this time dead embryo places 2-8 ℃ of preservation at once, carry out following step (4) after 120 hours and detect;
(4) all chicken embryos are got blastochyle (allantoic fluid and amniotic fluid) and measure blood clotting valency HA, if HA≤3log2 explains the complete of milipore filter film bag, if HA＞3log2 explains the imperfect of milipore filter film bag.
Embodiment 2
(1) at first new film bag is correctly installed according to the manufacturer's standard method of operating, is opened machine, treat that running is stable after, detect;
When (2) detecting; The antigen liquid that will contain 1% avian influenza virus that separates with 99% needs as the NDV that indicates antigen is got film bag diffusate 10ml through milipore filter film bag, and the penetrating fluid of taking-up is done HA detection (hemagglutination test (HA test)); Survey blood clotting valency HA; If HA＞3log2 explains that milipore filter film bag is imperfect, detect and finish; If HA≤3log2 proceeds following step (3) and detects;
(3) get 10 of the chicken embryos that ten ages in days do not have antibodies specific, wherein inoculation film bag diffusate 0.2ml in 8 every embryo allantois of embryo stay 2 pieces to compare; Inoculating back 37 ℃ cultivated 120 hours; Observe 2 time, if embryo non-specific death be no more than 1 according to the chicken embryo every day; Find that during this time dead embryo places 2-8 ℃ of preservation at once, carry out following step (4) after 120 hours and detect;
(4) all chicken embryos are got blastochyle (allantoic fluid and amniotic fluid) and measure blood clotting valency HA, if HA≤3log2 explains the complete of milipore filter film bag, if HA＞3log2 explains the imperfect of milipore filter film bag.
Embodiment 3
(1) at first new film bag is correctly installed according to the manufacturer's standard method of operating, is opened machine, treat that running is stable after, detect;
When (2) detecting,, get film bag diffusate 10ml with the antigen liquid process milipore filter film bag of NDV; The penetrating fluid that takes out is HA detects (hemagglutination test (HA test)), survey blood clotting valency HA, if HA＞3log2; Explain that milipore filter film bag is imperfect, detect and finish; If HA≤3log2 proceeds following step (3) and detects;
(3) get 10 of the chicken embryos that ten ages in days do not have antibodies specific, wherein inoculation film bag diffusate 0.2ml in 8 every embryo allantois of embryo stay 2 pieces to compare; Inoculating back 37 ℃ cultivated 120 hours; Observe 2 time, if embryo non-specific death be no more than 1 according to the chicken embryo every day; Find that during this time dead embryo places 2-8 ℃ of preservation at once, carry out following step (4) after 120 hours and detect;
(4) all chicken embryos are got blastochyle (allantoic fluid and amniotic fluid) and measure blood clotting valency HA, if HA≤3log2 explains the complete of milipore filter film bag, if HA＞3log2 explains the imperfect of milipore filter film bag.
In sum, content of the present invention is not confined in the above embodiments, and the knowledgeable people in the same area can propose other embodiment easily within technological guidance's thought of the present invention, but this embodiment is included within the scope of the present invention.
Claims (3)
1. the integrality detection method of a milipore filter film bag is characterized in that, may further comprise the steps:
(1) at first new film bag is correctly installed according to the manufacturer's standard method of operating, is opened machine, treat that running is stable after, detect;
When (2) detecting, the antigen liquid that will contain the antigen that separates with needs as the NDV that indicates antigen is got film bag diffusate through milipore filter film bag; The penetrating fluid that takes out is HA detects, survey blood clotting valency HA, if HA＞3log2; Explain that milipore filter film bag is imperfect, detect and finish; If HA≤3log2 proceeds following step (3) and detects;
(3) get 10 of the chicken embryos that ten ages in days do not have antibodies specific, wherein inoculation film bag diffusate 0.2ml in 8 every embryo allantois of embryo stay 2 pieces to compare; Inoculating back 37 ℃ cultivated 120 hours; Observe 2 time, if embryo non-specific death be no more than 1 according to the chicken embryo every day; Find that during this time dead embryo places 2-8 ℃ of preservation at once, carry out following step (4) after 120 hours and detect;
(4) all chicken embryos are got blastochyle and measure blood clotting valency HA, if HA≤3log2 explains the complete of milipore filter film bag, if HA＞3log2 explains the imperfect of milipore filter film bag.
2. the integrality detection method of milipore filter film bag according to claim 1 is characterized in that, the NDV antigen liquid is counted by volume and accounted for 0.5-1% in the said antigen liquid.
3. the integrality detection method of milipore filter film bag according to claim 1 is characterized in that, the said antigen that needs to separate is avian influenza virus or newcastle disease virus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110360538 CN102430340B (en) | 2011-11-15 | 2011-11-15 | Method for testing integrity of ultra-filtration membrane envelope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110360538 CN102430340B (en) | 2011-11-15 | 2011-11-15 | Method for testing integrity of ultra-filtration membrane envelope |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102430340A true CN102430340A (en) | 2012-05-02 |
| CN102430340B CN102430340B (en) | 2013-09-18 |
Family
ID=45978908
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110360538 Active CN102430340B (en) | 2011-11-15 | 2011-11-15 | Method for testing integrity of ultra-filtration membrane envelope |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102430340B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108507924A (en) * | 2018-03-30 | 2018-09-07 | 华中科技大学 | A kind of integrity of ultrafiltration membrane recognition methods and device |
| US10617603B2 (en) | 2016-01-22 | 2020-04-14 | Baxter International Inc. | Sterile solutions product bag |
| CN112386949A (en) * | 2020-09-18 | 2021-02-23 | 派特（北京）科技有限公司 | Clinical single-tube fluorine-18 multifunctional module equipment and radiopharmaceutical synthesis process |
| US11021275B2 (en) | 2016-01-22 | 2021-06-01 | Baxter International Inc. | Method and machine for producing sterile solution product bags |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5480554A (en) * | 1992-05-13 | 1996-01-02 | Pall Corporation | Integrity-testable wet-dry-reversible ultrafiltration membranes and method for testing same |
| CN2779386Y (en) * | 2005-04-08 | 2006-05-10 | 天津市兴源环境技术工程有限公司 | Membrane module integrity detector for membrane bioreactor |
| CN101341389A (en) * | 2005-12-20 | 2009-01-07 | 底格里蒙公司 | Method and device for testing the integrity of filtration membranes |
| CN101762561A (en) * | 2009-11-10 | 2010-06-30 | 浙江天元生物药业股份有限公司 | Method for on-line detecting integrity of ultrafiltration membrane by using ultraviolet monitoring method |
-
2011
- 2011-11-15 CN CN 201110360538 patent/CN102430340B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5480554A (en) * | 1992-05-13 | 1996-01-02 | Pall Corporation | Integrity-testable wet-dry-reversible ultrafiltration membranes and method for testing same |
| CN2779386Y (en) * | 2005-04-08 | 2006-05-10 | 天津市兴源环境技术工程有限公司 | Membrane module integrity detector for membrane bioreactor |
| CN101341389A (en) * | 2005-12-20 | 2009-01-07 | 底格里蒙公司 | Method and device for testing the integrity of filtration membranes |
| CN101762561A (en) * | 2009-11-10 | 2010-06-30 | 浙江天元生物药业股份有限公司 | Method for on-line detecting integrity of ultrafiltration membrane by using ultraviolet monitoring method |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10617603B2 (en) | 2016-01-22 | 2020-04-14 | Baxter International Inc. | Sterile solutions product bag |
| US11021275B2 (en) | 2016-01-22 | 2021-06-01 | Baxter International Inc. | Method and machine for producing sterile solution product bags |
| US11564867B2 (en) | 2016-01-22 | 2023-01-31 | Baxter International Inc. | Sterile solutions product bag |
| US11623773B2 (en) | 2016-01-22 | 2023-04-11 | Baxter International Inc. | Method and machine for producing sterile solution product bags |
| CN108507924A (en) * | 2018-03-30 | 2018-09-07 | 华中科技大学 | A kind of integrity of ultrafiltration membrane recognition methods and device |
| CN108507924B (en) * | 2018-03-30 | 2023-08-04 | 华中科技大学 | Ultrafiltration membrane integrity recognition method and device |
| CN112386949A (en) * | 2020-09-18 | 2021-02-23 | 派特（北京）科技有限公司 | Clinical single-tube fluorine-18 multifunctional module equipment and radiopharmaceutical synthesis process |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102430340B (en) | 2013-09-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DiNapoli et al. | Immunization of primates with a Newcastle disease virus-vectored vaccine via the respiratory tract induces a high titer of serum neutralizing antibodies against highly pathogenic avian influenza virus | |
| Stöhr et al. | Influenza virus surveillance, vaccine strain selection, and manufacture | |
| CN102430340B (en) | Method for testing integrity of ultra-filtration membrane envelope | |
| CN106596933B (en) | Newcastle disease virus antibody test blocks ELISA kit | |
| CN102078605B (en) | Method for preparing Vero cell influenza virus vaccine | |
| KR101963983B1 (en) | High throughput quantification and characterization of viruses and products thereof | |
| CN101491672A (en) | Chicken new castle disease-bird influenza dual oil-emulsion inactivated vaccine and preparation method thereof | |
| CN102520169A (en) | ELISA (Enzyme-Linked Immuno Sorbent Assay) detection kit of animal rabies neutralizing antibody and application thereof | |
| CN101348777B (en) | Influenza A virus ELISA nucleoprotein capture antigen diagnose reagent kit and special monoclonal antibody therefor | |
| CN103937754B (en) | Porcine reproductive and respiratory syndrome virus (PPRSV) purification method | |
| CN111537714B (en) | Freeze-dried hemagglutination inhibition test antigen for detecting chicken mycoplasma synoviae, antigen combination and preparation method | |
| CN103604924A (en) | Canine parvovirus colloidal gold immunochromatography test strip and preparation method | |
| CN109239342A (en) | QX type IBV hemagglutination-inhibition test antigen and the preparation method and application thereof | |
| CN102344972B (en) | H6 subtype avian influenza virus RT-LAMP (reverse transcription loop-mediated isothermal amplification) kit | |
| CN102018955A (en) | Purification method for producing viral vaccine in large scale | |
| CN102721812B (en) | Indirect ELISA (enzyme-linked immuno-sorbent assay) kit for detecting nephropathogenic avian infectious bronchitis virus and antibody thereof | |
| CN107254450A (en) | The chimeric Newcastle Disease Virus Vaccine carrier Candidate Strain and construction method for overcoming newcastle disease maternal antibody to influence | |
| Ray et al. | Glycoproteins of human parainfluenza virus type 3: affinity purification, antigenic characterization and reconstitution into lipid vesicles | |
| CN104911150B (en) | The foundation of monoclonal antibody hybridoma cell strain and its preparation and application of monoclonal antibody of a kind of H3N2 canine influenza virus | |
| CN104744590B (en) | Anti-H 1 N 1 swine influenza virus hemagglutinin monoclonal antibody and double crush syndrome kit | |
| CN106771173A (en) | The monoclonal antibody of anti-H9 subtype avian influenza virus, hybridoma cell strain and its application | |
| US9701946B2 (en) | Purification method for embryo—derived infectious bronchitis virus (IBV) | |
| CN107779441B (en) | Concentration and purification method of avian influenza antigen and avian influenza antigen | |
| CN107064488A (en) | A kind of total antibody solid phase of CSFV serum blocks the preparation method of antigen used in ELISA kit | |
| CN101000340B (en) | Method of biochip for simultaneous testing avian influenza infection of human and fowls |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: TIANJIN RUIPU BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: TIANJIN RINGPU GAOKE BIOPHARMACEUTICAL CO., LTD. Effective date: 20120919 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 300380 XIQING, TIANJIN TO: 300308 BINHAI NEW DISTRICT, TIANJIN |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20120919 Address after: 300308 Tianjin Binhai New Area Airport Economic Zone Ring Road No. 76 West Airport Business District W2 Applicant after: Tianjin Ruipu Biotechnology Co., Ltd. Address before: 300380, No. 6, Taixing Road, Xin Dao Town Industrial Park, Xiqing District, Tianjin Applicant before: Tianjin Ringpu Bio-Technology Co., Ltd. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20120502 Assignee: Hunan Sinoland Biological Pharmaceutical Co., Ltd. Assignor: Tianjin Ruipu Biotechnology Co., Ltd. Contract record no.: 2014120000052 Denomination of invention: Method for testing integrity of ultra-filtration membrane envelope Granted publication date: 20130918 License type: Exclusive License Record date: 20140708 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| EC01 | Cancellation of recordation of patent licensing contract | ||
| EC01 | Cancellation of recordation of patent licensing contract |
Assignee: Hunan Sinoland Biological Pharmaceutical Co., Ltd. Assignor: Tianjin Ruipu Biotechnology Co., Ltd. Contract record no.: 2014120000052 Date of cancellation: 20180628 |