CN102430267A - High flow middle and low voltage constant-current stabilizer for preparing liquid chromatogram and application method thereof - Google Patents
High flow middle and low voltage constant-current stabilizer for preparing liquid chromatogram and application method thereof Download PDFInfo
- Publication number
- CN102430267A CN102430267A CN2011102754688A CN201110275468A CN102430267A CN 102430267 A CN102430267 A CN 102430267A CN 2011102754688 A CN2011102754688 A CN 2011102754688A CN 201110275468 A CN201110275468 A CN 201110275468A CN 102430267 A CN102430267 A CN 102430267A
- Authority
- CN
- China
- Prior art keywords
- constant
- current stabilizer
- flow
- liquid chromatography
- preparative liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention relates to a high flow middle and low voltage constant-current stabilizer for preparing liquid chromatogram, particularly a high flow middle and low voltage constant-current stabilizer filled with elastic materials for preparing liquid chromatogram and an application method thereof. The application method is characterized in that: when the flow of a used infusion pump is unstable, if the flow (pressure) is high, the elastic materials in the device are extruded, the deformation is increased, a certain volume of flow is consumed; on the contrary, if the flow (pressure) is low, the deformation of the elastic materials is decreased, the flowing phase in the constant-current stabilizer is extruded to compensate the flow, thus the effect of stabilizing current is achived. According to the invention, by using the elastic materials to buffer the flow and pressure of the flowing phase conveyed by the infusion pump, the pulsation of the infusion pump is effectively weakened, and the stable current is acquired. The device has the advantages of simple structure, easy operation, flexible application, low cost, and can greatly reduce the production cost.
Description
Technical field
The present invention relates to a kind of preparative liquid chromatography constant-current stabilizer, a kind of specifically big flow mesolow preparative liquid chromatography constant-current stabilizer and method for using thereof.
Background technology
Preparative chromatography is the human so far a kind of method of grasping higher to the complex mixture separative efficiency.Preparative chromatography is becoming the more and more important means of separating aspect the production of medical industry, biochemical technology and fine chemicals.Under many situation, required high-purity standard specimen can only obtain with the preparative chromatography technology, like separation of racemates.Along with bioengineering and the modern an urgent demand of traditional Chinese medicine, all be faced with a large amount of separation and purification tasks in relevant field, preparative chromatography has caused the great attention of countries in the world as a kind of isolation technics rapidly and efficiently.Preparing the transfusion pump performance as preparation system medium power supplier has crucial effects to separative efficiency and fractional dose, and the stability of mobile phase flow rate influences product purity and output to a certain extent.
In order to make the discharge stability of infusion pump in the preparative liquid chromatography reach the requirement of high-performance preparative liquid chromatography; Usually adopt two ball seat check valves in the market; Pulsation damper or damping limiter are installed between reciprocating pump and injector; Be used for weakening the pulsation of pump self, also use the modern electro sub-control system simultaneously, the regulating piston stroke frequency is with the designs such as pulsation of automatic compensation transfusion.But this type of device, the cost cost is high, and it is difficult to safeguard, is not suitable for using in the middle of the commercial production life.In order to satisfy industrial requirement, people have done a lot of improvement to liquid phase pump.How to reduce cost, process the focus that simple and effective constant-current stabilizer becomes current research.
Macromolecular material has elastic deformation preferably, under the situation that receives extraneous extruding, can the oneself shrink, and produces a reaction force to external world simultaneously, forms a resistance.When ambient pressure was removed, because the elastic deformation effect, elastomeric material restPosed, and produced a power simultaneously to external world.Utilize this specific character of elastomeric material, it is applied in the preparation liquid phase infusion pump constant-current stabilizer can not only reaches the effect of current stabilization, and reduction manufacturing cost that can be bigger.
Summary of the invention
The technical problem that the present invention will solve is: a kind of simple, effectively big flow mesolow preparative liquid chromatography constant-current stabilizer and method for using thereof are provided.This device consumes and compensates flow or pressure based on the elastic deformation characteristics of elastomeric material self, thereby effectively weakens the pulsation of infusion pump, obtains stable flow rate, reaches the requirement of high precision high flow rate mesolow preparative liquid chromatography.
The technical solution adopted for the present invention to solve the technical problems is: a kind of big flow mesolow preparative liquid chromatography constant-current stabilizer, form by closed container and inner elastomeric material of filling thereof.Elastomeric material has the better elastic deformation behavior, is graininess, or for being coated on container inner wall, perhaps being filled in the closed container for porous form.
Said closed container comprises container body and complete container lid, and both combine with screw thread, and closed container can be cylindricality, sphere and different shape such as square.The two ends of closed container have inlet and liquid outlet, link to each other with the preparative liquid chromatography system through pipeline.
Said closed container volume V=1mL～10000mL, concrete size is corresponding with flow.
Said elastomeric material can adopt the organic and inorganic preferably or organic inorganic hybridization macromolecular material of rubber, polyurethane, silicon rubber and other elastic deformation; This type of material is prone to obtain; Cost is low, and chemical property is stable, but acid and alkali resistance and the corrosion of various organic solvent; Long service life can be used repeatedly.
The method for using of said big flow mesolow preparative liquid chromatography constant-current stabilizer is: certain particle size size flexibility material is filled in the constant-current stabilizer; Again constant-current stabilizer is linked to each other with the preparative liquid chromatography infusion pump with pipeline through inlet; Flowing phase gets into constant-current stabilizer by infusion pump output back; After the buffering through elastomeric material wherein, obtain stable flow rate.Be specially: if the infusion pump instability of flow, flow (pressure) is bigger than normal, and elastomeric material is squeezed in the device, and deformation increases, and consumes a part of flow, reduces the liquid that flows out; Otherwise flow (pressure) is less than normal, and elastomeric material deformation reduces, and the flowing phase in the extruding constant-current stabilizer gives infusion pump with flow-compensated, thereby reaches the effect of current stabilization.In order to obtain more stable flow rate, reach the requirement of high-performance preparative liquid chromatography simultaneously, a plurality of constant-current stabilizers of also can in same system, connecting form multi-buffer.
Said elastomeric material can also be inserted multiple elastomeric material with difformity, different grain size and different elasticity capacities according to flowing phase character and the concrete selection of pressure size in same constant-current stabilizer.
The present invention has the following advantages and effect:
The first, the present invention can weaken largely because the instability of flow that the infusion pump pulsation causes.One or more these constant-current stabilizers of series connection utilize the elastomeric material elastic deformation characteristics behind the preparation infusion pump, form flow and pressure buffer, thereby obtain stable flow rate.
The second, install simple and easyly, be easy to safeguard.Form by simple closed container and inner elastomeric material two parts thereof, and the dress of elastomeric material to get process simple.
The 3rd, use flexibly.Can in constant-current stabilizer, fill the elastomeric material particle of appropriate amount difformity, different grain size size and different elasticity capacities, a plurality of constant-current stabilizers of also can behind infusion pump, connecting according to flow.
The 4th, low cost of manufacture, and can reach high-performance infusion pump same effect behind the difference performance infusion pump process current stabilization of the present invention, reduce production costs greatly.
The 5th, promotional value is high.This device can be used in the big flow mesolow preparative liquid chromatography, can be used in fields such as biology, pharmacy, chemical industry preferably.
Description of drawings
Below in conjunction with accompanying drawing and embodiment the present invention is further specified.
Fig. 1 is a constant-current stabilizer structural representation of the present invention;
Fig. 2 is the constant-current stabilizer working state schematic representation of embodiment 1;
Fig. 3 is the constant-current stabilizer tandem working view of embodiment 2;
Fig. 4 is not for using the chromatogram of this stream device;
Fig. 5 is for using the chromatogram of this stream device.
Among the figure: 1, closed container; 2, inlet; 3, liquid outlet; 4, metal closed container main body; 5, elastomeric material; 6, container cover; 7, constant-current stabilizer; 8, pipeline; 9, pump.
The specific embodiment
Embodiment 1
As illustrated in fig. 1 and 2, a kind of big flow mesolow preparative liquid chromatography constant-current stabilizer and method for using thereof.Constant-current stabilizer 7 is made up of closed container 1 and elastomeric material 5.Closed container 1 is made up of cylindricality metal closed container main body 4 and container cover 6, and the two to be being threaded, between be placed with sealing ring.Elastomeric material 5 places in the closed container 1.Closed container 1 two ends have inlet 2 and liquid outlet 3, and inlet 2 links to each other with pump 9 through pipeline 8.Closed container 1 volume is 100ml, and three kinds of elastic performance different elasticity materials 5 are equipped with in inside.
Big flow infusion pump 9 is directly linked to each other with the UV detector, is flowing phase with 0.5% aqueous acetone solution, at flow 180ml/min, back pressure 1Mpa, detection wavelength 254nm condition operational system, record gained chromatogram.Then with the constant-current stabilizer 7 of connecting between big flow infusion pump 9 and the detector, operational system under the same conditions, record chromatogram.The gained result is as shown in Figure 4, does not add constant-current stabilizer 7 gained chromatograms and has tangible fluctuation, stable RSD>=5.0%; After the series connection constant-current stabilizer 7, fluctuation reduces greatly, stable RSD≤1%.
Embodiment 2
As shown in Figure 3, a kind of big flow mesolow preparative liquid chromatography constant-current stabilizer and method for using thereof.Constant-current stabilizer 7 is made up of the closed container 1 and the inner elastomeric material 5 thereof of three series connection.Closed container 1 is made up of cylindricality metal closed container main body 4 and container cover 6, and the two to be being threaded, between be placed with sealing ring.Elastomeric material 5 is filled in the closed container 1.Closed container 1 two ends have inlet 2 and liquid outlet 3, and inlet 2 links to each other with pump 9 through pipeline 8.Closed container 1 volume is 60ml.
Big flow infusion pump 9 is directly linked to each other with the UV detector, is flowing phase with 0.5% aqueous acetone solution, in the condition operational system of flow 120ml/min, back pressure 1Mpa, detection wavelength 254nm, investigates the stability of a system.With three constant-current stabilizers 7 of connecting between big flow infusion pump 9 and the detector, operational system is investigated the stability of a system under the same conditions then.The result shows that adding the constant-current stabilizer 7 back stabilities of a system is greatly improved.
The above is a kind of occupation mode of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the present invention's design; Can also make some this and advance and retouch, these improvement and retouching also should be regarded as in protection scope of the present invention.
Claims (6)
1. one kind big flow mesolow preparative liquid chromatography constant-current stabilizer is characterized in that: be made up of closed container and the inner elastomeric material that loads thereof.
2. big flow mesolow preparative liquid chromatography constant-current stabilizer according to claim 1 is characterized in that: said closed container smooth interior, and volume V=1mL～10000mL, concrete size is corresponding with flow; Two ends have inlet and liquid outlet, link to each other with the preparative liquid chromatography system through pipeline.
3. big flow mesolow preparative liquid chromatography constant-current stabilizer according to claim 1 is characterized in that: described elastomeric material is graininess, or for being coated on container inner wall, or being to be filled in porous form in the closed container.
4. big flow mesolow preparative liquid chromatography constant-current stabilizer according to claim 3; It is characterized in that: described elastomeric material has better elastic and chemical stability, can have the organic and inorganic of favorable elasticity and chemical stability or organic inorganic hybridization macromolecular material for rubber, polyurethane, silicon rubber and other.
5. the method for using of big flow mesolow preparative liquid chromatography constant-current stabilizer according to claim 1; It is characterized in that: behind the big flow infusion pump of poor stability; Directly the series connection constant-current stabilizer; Flowing phase gets into constant-current stabilizer by infusion pump output back, through after the buffering of elastomeric material, obtains stable flow rate.
6. the method for using of big flow mesolow preparative liquid chromatography constant-current stabilizer according to claim 5 is characterized in that: not only can in same constant-current stabilizer, insert multiple elastomeric material, a plurality of constant-current stabilizers of can also in a system, connecting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110275468.8A CN102430267B (en) | 2011-09-16 | 2011-09-16 | High flow middle and low voltage constant-current stabilizer for preparing liquid chromatogram and application method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110275468.8A CN102430267B (en) | 2011-09-16 | 2011-09-16 | High flow middle and low voltage constant-current stabilizer for preparing liquid chromatogram and application method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102430267A true CN102430267A (en) | 2012-05-02 |
| CN102430267B CN102430267B (en) | 2014-12-10 |
Family
ID=45978838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110275468.8A Expired - Fee Related CN102430267B (en) | 2011-09-16 | 2011-09-16 | High flow middle and low voltage constant-current stabilizer for preparing liquid chromatogram and application method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102430267B (en) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4032445A (en) * | 1975-11-10 | 1977-06-28 | Varian Associates | Liquid chromatography pumping system with compensation means for liquid compressibility |
| EP0222921A1 (en) * | 1985-05-13 | 1987-05-27 | Showa Denko Kabushiki Kaisha | Apparatus for preventing pulsating current for liquid chromatography and method therefor |
| CN2142200Y (en) * | 1992-12-24 | 1993-09-15 | 北京市理化分析测试中心 | Diaphragm pressure fluctuation buffer |
| CN2221116Y (en) * | 1994-08-31 | 1996-02-28 | 李玉华 | Continuous liquid feed pump |
| CN2861670Y (en) * | 2005-11-03 | 2007-01-24 | 刘永茂 | Automatic constant current stabilizer |
| CN2883877Y (en) * | 2006-02-13 | 2007-03-28 | 卢峰 | Current regulator for liquid conveying duct |
| CN101059486A (en) * | 2007-05-10 | 2007-10-24 | 复旦大学 | Hot expansion continuous microflow high pressure gradient pump for high-performance liquid chromatographer |
| EP1887353A1 (en) * | 2005-05-17 | 2008-02-13 | Surpass Industry Co., Ltd. | Damper |
| CN101701615A (en) * | 2009-11-20 | 2010-05-05 | 南京航空航天大学 | Bellow-type mixed medium vibration isolating and absorbing unit |
| CN101750450A (en) * | 2008-12-17 | 2010-06-23 | 中国科学院大连化学物理研究所 | Automatic sampling device for array capillary electrophoresis |
| CN202398145U (en) * | 2011-09-16 | 2012-08-29 | 常州博世伟业生物科技有限公司 | Large-flow medium and low-pressure current stabilizer for preparation of liquid chromatography |
-
2011
- 2011-09-16 CN CN201110275468.8A patent/CN102430267B/en not_active Expired - Fee Related
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4032445A (en) * | 1975-11-10 | 1977-06-28 | Varian Associates | Liquid chromatography pumping system with compensation means for liquid compressibility |
| EP0222921A1 (en) * | 1985-05-13 | 1987-05-27 | Showa Denko Kabushiki Kaisha | Apparatus for preventing pulsating current for liquid chromatography and method therefor |
| CN2142200Y (en) * | 1992-12-24 | 1993-09-15 | 北京市理化分析测试中心 | Diaphragm pressure fluctuation buffer |
| CN2221116Y (en) * | 1994-08-31 | 1996-02-28 | 李玉华 | Continuous liquid feed pump |
| EP1887353A1 (en) * | 2005-05-17 | 2008-02-13 | Surpass Industry Co., Ltd. | Damper |
| CN2861670Y (en) * | 2005-11-03 | 2007-01-24 | 刘永茂 | Automatic constant current stabilizer |
| CN2883877Y (en) * | 2006-02-13 | 2007-03-28 | 卢峰 | Current regulator for liquid conveying duct |
| CN101059486A (en) * | 2007-05-10 | 2007-10-24 | 复旦大学 | Hot expansion continuous microflow high pressure gradient pump for high-performance liquid chromatographer |
| CN101750450A (en) * | 2008-12-17 | 2010-06-23 | 中国科学院大连化学物理研究所 | Automatic sampling device for array capillary electrophoresis |
| CN101701615A (en) * | 2009-11-20 | 2010-05-05 | 南京航空航天大学 | Bellow-type mixed medium vibration isolating and absorbing unit |
| CN202398145U (en) * | 2011-09-16 | 2012-08-29 | 常州博世伟业生物科技有限公司 | Large-flow medium and low-pressure current stabilizer for preparation of liquid chromatography |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102430267B (en) | 2014-12-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20120175382A1 (en) | Liquid Container | |
| CN202398145U (en) | Large-flow medium and low-pressure current stabilizer for preparation of liquid chromatography | |
| CN103230704A (en) | Composite type filter for water treatment system | |
| CN204411989U (en) | A kind of gas/liquid separator device | |
| CN106837754A (en) | Multi-phase pipeline membrane pump | |
| CN102430267B (en) | High flow middle and low voltage constant-current stabilizer for preparing liquid chromatogram and application method thereof | |
| US9897578B2 (en) | Chromatography columns | |
| CN102784502A (en) | Nested composite filter core | |
| CN209671782U (en) | A kind of resistance to compression tubing | |
| CN203678392U (en) | Metal intalox saddle packing | |
| CN202950362U (en) | Reinforced sales promotion cage | |
| CN202440541U (en) | Distributor sieve plate device | |
| CN202100839U (en) | Inner pipe structure of resin pipes | |
| CN208292796U (en) | A kind of Pure water purification device | |
| CN202327256U (en) | Constant-flow non-return valve | |
| CN202179705U (en) | Piston type conveying container of liquid medicine | |
| CN202453332U (en) | High performance liquid-phase preparative chromatographic column | |
| CN202605779U (en) | Liquid phase chromatogram separation column | |
| CN102062765B (en) | Column head structure of conical preparative liquid chromatographic column | |
| CN205479543U (en) | One way check valve | |
| CN209041985U (en) | The seal nipple of high-pressure fluid tank, high-pressure fluid infusion device | |
| CN205770855U (en) | A kind of Novel toothpaste tube | |
| CN205837506U (en) | A kind of sebific duct assembly filling gluey pesticide | |
| CN202671194U (en) | Poisonous chemical material discharging device for chemical fiber production | |
| CN105135010B (en) | A kind of magnetic one-way valve |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141210 Termination date: 20200916 |