CN102405834A - Rapid propagation method of Tianjiao - Google Patents
Rapid propagation method of Tianjiao Download PDFInfo
- Publication number
- CN102405834A CN102405834A CN2011102415034A CN201110241503A CN102405834A CN 102405834 A CN102405834 A CN 102405834A CN 2011102415034 A CN2011102415034 A CN 2011102415034A CN 201110241503 A CN201110241503 A CN 201110241503A CN 102405834 A CN102405834 A CN 102405834A
- Authority
- CN
- China
- Prior art keywords
- seedling
- bud
- culture
- aseptic
- sweet angle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
A rapid propagation method of Tianjiao (a plant) comprises the following steps: refrigerating and sterilizing the seeds of the clean Tianjiao; inoculating on the aseptic seedling culture medium of to induce and produce the aseptic seedlings; inoculating the leaves of the aseptic seedlings to callus inductive differentiation culture medium, then obtain the adventitious buds, and inoculating to enrichment medium; after the adventitious buds are grown through the propagation cultivation, inoculating to rooting culture medium to execute the rooting cultivation, and obtaining the regeneration plants of the Tianjiao through seedling the raw seedlings. The invention can effectively induce the adventitious buds, and has the characteristics of small heteromorphosis and high genetic stability. The survival rate is over 90%, so the aim at fast propagation can be realized.
Description
Technical field
The invention belongs to plant method for culturing in-vitro tissues technical field, particularly the method for quickly breeding at the sweet angle of plant.
Background technology
Tamarind has another name called (Chinese Plants will such as tamarind, tamarind, tamarind (Tamarindus indica Linn.), tamarind; Beijing 1988.39); Belonging to a kind of evergreen megaphanerophyte that Caesalpiniaceae (Caesalpiniaceae) tamarind belongs to (Tamarindus Linn.), is single plant species that belongs to.Tamarind originates in the torrid areas in African east and Asia; Very wide in torrid areas and the distribution of south subtropics area, all there is distribution on ground such as China each province, south such as Taiwan, Hainan, Fujian, Guangdong, Guangxi, Sichuan, Yunnan, Guizhou, are many with Hainan especially; The domestic tamarind overwhelming majority is in fragmentary wild or semi-wild state; The area estimation is less than 830 hectares, and a large amount of old tamarind trees are cut down, and resource is damaged serious.Sweet angle is a kind of of tamarind, and the fruit taste is sour-sweet, thick flavor, and nutritive value is very high, and total sugar content 45.90%～50.26% in the pulp, and content of reducing sugar 33.66%～46.94% is far above the higher citrus of sugar content; Crude protein and Vc content also contain 17 seed amino acids near pears and apple etc. in the fruit, wherein 7 kinds of essential amino acids account for 45.85% (Zhao Yihe etc. of total amino acid content; " Thailand's sweet angle different cultivars nutrient constituents of fruit is analyzed " plant resources and environment journal; 2005,14 (3)), also be rich in multiple mineral element; Content of elements such as its calcium, phosphorus are apparently higher than general fruit, and ratio balance (Ca:P=1:1).There are some researches show that sweet angle pulp has bacterium, hypoglycemic, mutation and the effects (Zhao Yihe etc., " world's tamarind present Research and progress " Yunnan Prov Agriculture University's journal, 2005 (1)) such as anticarcinogen, protection cellular damage of suppressing; Tamarind gum content in the seed of sweet angle reaches 58.5%～65.0%; For a kind of similar pectin but performance is superior to the good food thickening agent and the stabilizing agent of pectin again; Be used to improve mouthfeel and stability, the enhancing goods of baste stiff sense and sweet taste, make particle not free settling etc. have good effect; The industries such as food, medicine, weaving, latex, building, timber, adhesive, explosive, papermaking, grease and daily use chemicals of a lot of developed countries are all used tamarind gum (Wang Yuanlan etc. more and more widely in the world; " tamarind gum and in Application in Food Industry " food research and development, 2006 (9)); The kind micromicro at sweet angle adds pigment to extract antioxidant and food; Protein content reaches 16.85%～19.50% in the seed, can be used for processing the legumin feed; Sweet angle leaf can be made the bleaching agent of drinking water, and it is edible also can to do vegetables; Sweet angle block material ground hard and compact, the sapwood yellow-white, the black purple of heartwood is with brown, is described as " Madeira redwood "; Sweet hornbeam tree type is graceful, and branches and leaves are evergreen, and the flower amount is big; Florescence is long, and 4～8 monthlyly constantly bloom, and are that a kind of desirable garden is viewed and admired arbor; If treelet is imposed gardening potted landscape technology, be a kind of first-class bonsai production material and view and admire tree, be planted in by park, garden, avenue, the road; Play the effect (Zhao Yihe etc., " Thailand's sweet angle introduction and cultivation " Zhejiang forestry science and technology, 2005 (1)) of viewing and admiring and shading.Sweet angle has more and more received people's attention as integrating the multiduty resource plant that fruit is used, leaf is used, material is used and viewed and admired.
The breeding at present sweet angle adopts grow directly from seeds breeding or propagation by grafiting, and there is the problem of two aspects in the propagation method of growing directly from seeds, and the one, the tamarind seed under field conditions (factors) can not long term storage; Subject to insect pest, hard exosper makes its germination rate very low and germinating time is long, and the 2nd, the method for the breeding of growing directly from seeds receives the influence of season limit; Reproduction speed is also slower; Growing directly from seeds to grow seedlings as Thailand Tian Jiao needs 10～12 years solid evenings of just bearing fruit, and exist, unstable, the fruit pod quality deterioration of output, and branch is tall and big, pluck and problem such as be difficult for; In addition, the offspring of seedling separation also is a big problem.The propagation by grafiting technical research at sweet angle starts from the nineties in 20th century, and the expert possesses some special knowledge to the aspects such as grafting method, grafting time and seedling age effect at sweet angle, is the main path of present sweet angle breeding; Be characterized in keeping maternal merit and speed to give birth to etc., propagation by grafiting afforestation is compared with the breeding of growing directly from seeds, and can carry and several years ago bearing fruit; But different regions are owing to reasons such as kind, weathers; Graft technology also there are differences, different grafting periods and grafting method, and graft survival rate exists than big-difference.Under the weather conditions of South China, better with cut-grafting in spring method, graft survival rate is about 80%, and autumn in summer high temperature and drought survival rate in season is lower; And the simple bud side grafting is because the tamarind alkaloid is higher; Cortex is thin, sprout is little; Cut bud and peeling and all be difficult for, therefore, graft survival rate is lower; Xiao Yan, Zhao Yihe etc. show that to the grafting experiment at the sweet angle of Thailand the average survival of the cut-grafting method propagation by grafiting that effect is best also only reaches 75.0%～76.4%.
Utilize the in vitro tissue cultured method that sweet angle is bred fast; Can not receive the influence of natural conditions to carry out batch production produces; Can break the restriction in season, obtain the regeneration plant that variation is little, genetic stability is high fast, 450,000 plant species are arranged in the world; Surplus wherein higher plant has 20 approximately ten thousand kinds, there is kind surplus the higher plant 30,000 in China.The success of the test of group training at present have only the hundreds of kind; Variety classes; Different cultivars and genotype are slightly different; The kind and the proportioning of used minimal medium of each stage of group training, plant hormone are also inequality, and how making the not only efficient but also practicality of the tissue culture at sweet angle is the key problem in technology that tissue-culturing quick-propagation is carried out at sweet angle.
Summary of the invention
The method that the object of the present invention is to provide a kind of sweet angle in vitro tissue to cultivate and breed fast makes sweet angle realize large-scale industrialized production.
The technical scheme that the present invention adopts is: will select the sweet angle seed refrigeration sterilization treatment of wash clean earlier, and then seed will be inoculated in the aseptic seedling medium, nutrient media components is: MS minimal medium+6-BA 0.8～1.2mg/L+NAA0.1～0.5mg/L; Cultivate aseptic seedling; The cotyledon stripping and slicing youngster of aseptic seedling is inoculated into callus induction-differential medium, and nutrient media components is MS minimal medium+6-BA7～10mg/L+ Coconut Juice 18～22ml/L, induces to produce callus and differentiate the differentiation bud; The differentiation bud that obtains changes proliferated culture medium over to; Nutrient media components is MS minimal medium+6-BA0.8～1.2mg/L+IBA0.3～0.8mg/L+ active carbon 0.3～0.5g/L, turns out indefinite bud, and less indefinite bud is cut into the segment of 1-2 joint; Be inoculated in proliferated culture medium once more; Continue the Culture and Differentiation indefinite bud, bigger indefinite bud inserts root media, and nutrient media components is 1/2MS+ NAA0.3～0.5mg/L+ active carbon 0.3～0.5g/L; Culture of rootage obtains the seedling of taking root, and the seedling of taking root forms regeneration plant through refining seedling, transplanting.
The refrigeration of sweet angle seed is treated in 3～6 ℃ of refrigerating chambers and refrigerates 15 days.
Sweet angle seed sterilizing methods is: the seed after choosing is washed, refrigerated is with running water flushing 3～4h; Alcoholic solution with 75% volume fraction soaks 2～3min; Take out the back with aseptic water washing 3～5 times; Soak 10～15min with the mercuric chloride solution of 0.1% mass fraction again, take out the back with aseptic water washing 5 times.
Aseptic seedling is cultivated, the differentiation bud is cultivated, indefinite bud is cultivated identical with the condition of culture of culture of rootage: 23～26 ℃ of cultivation temperature, light application time 11～13h/d, intensity of illumination 2500Lux.
Keep 22 ± 2 ℃ of humidity 80～90%, temperature during the refining seedling.
The aseptic seedling incubation time is 20-23d, and differentiation bud incubation time is 20-25d, and the indefinite bud incubation time is 35-45d, and the culture of rootage time is 28-32d, refining seedling time 2-3d.
Technical scheme of the present invention realizes through following steps:
Step 1, seed treatment: the sweet angle seed that will select is removed pulp, and water is rinsed well, is placed on refrigeration taking-up after 15 days in 3～6 ℃ of refrigerating chambers.
Step 2; Seed sterilization: the seed after choosing washed, refrigerates is with running water flushing 3～4h, soaks 2～3min with the alcoholic solution of 75% volume fraction, after taking out with aseptic water washing 3～5 times; Soak 10～15min with the mercuric chloride solution of 0.1% mass fraction again, take out the back with aseptic water washing 5 times.
Step 3; Aseptic seedling is cultivated: the seed after will sterilizing is inoculated in the aseptic seedling medium, and nutrient media components is MS minimal medium+6-BA 0.8～1.2mg/L+NAA0.1～0.5mg/L, and condition of culture is: 23～26 ℃ of temperature; Light application time 11～13h/d; Intensity of illumination 2500Lux turns out aseptic seedling, incubation time 20～23d.
Step 4, the differentiation bud is cultivated: the aseptic seedling growth was got the fritter that cotyledon is cut into 0.3～0.5cm after 3-5 days; Be seeded on callus induction-differential medium; Nutrient media components is MS minimal medium+6-BA 7～10mg/L+ Coconut Juice 18～22ml/L, and the same step 3 of condition of culture induces to produce callus and differentiate the differentiation bud; Incubation time 20-25d, every callus can obtain 2-4 differentiation bud.
Step 5, indefinite bud is cultivated: will break up bud and change proliferated culture medium over to, nutrient media components is MS minimal medium+6-BA0.8～1.2mg/L+IBA0.3～0.8mg/L+ active carbon 0.3～0.5g/L; Carry out indefinite bud and cultivate the same step 3 of condition of culture, incubation time 35-45d; Select bigger indefinite bud and directly be used for culture of rootage, less indefinite bud is cut into the segment of 1-2 joint, is inoculated in proliferated culture medium once more; Continue to cultivate indefinite bud, can obtain a large amount of indefinite buds repeatedly.
Step 6, culture of rootage: will select bigger indefinite bud and change root media over to, nutrient media components is 1/2MS+ NAA0.3～0.5mg/L+ active carbon 0.3～0.5g/L; Carry out culture of rootage; The same step 3 of condition of culture, incubation time 28-32d obtains the seedling of taking root.
Step 7, refining seedling, transplanting: the seedling bottleneck of will taking root is opened, and it is contacted with air; Keep 22 ± 2 ℃ of humidity 80～90%, temperature, refining seedling time 2-3d is then with the take root medium of seedling base portion of flowing water flush away; Take out after soaking 1～2min with the liquor potassic permanganate of 0.1% mass fraction, be transplanted in the cultivation matrix of sterilization and cultivate, cultivation matrix is peat soil: vermiculite=2:1; Obtain regeneration plant, the regeneration plant survival rate reaches more than 90%, from indefinite bud to regeneration plant 65-80d.
Use method for tissue culture of the present invention that sweet angle is bred fast, broken the restriction in season, do not receive the influence of natural conditions, realize the batch production production at sweet angle, belong to foundation phase from seed treatment to differentiation bud cultivation stage in the technical scheme; One step of differentiation of the generation of evoked callus and differentiation bud accomplishes, and needs twice cultured method to compare with the evoked callus of routine and the differentiation of differentiation bud, has reduced by a step, has obviously shortened the time; After the differentiation bud changes proliferated culture medium over to; Bigger indefinite bud directly changes culture of rootage over to, and less indefinite bud is cut into the segment of 1-2 joint, is inoculated in proliferated culture medium once more; Continue to cultivate indefinite bud; Can obtain a large amount of indefinite buds repeatedly, from the indefinite bud to the regeneration plant, only need to have realized obtaining in a large number, fast the purpose of regeneration plant about 2 first quarter moons; The regeneration plant survival rate reaches more than 90%, and has the little and high advantage of genetic stability of regeneration plant variation; The callus that the present invention technology produces also can be used as the genetically modified acceptor in sweet angle, use among the present invention break up bud, indefinite bud, the culture technique of take root seedling and regeneration plant obtains sweet angle transfer-gen plant.
Description of drawings
Fig. 1 is the aseptic seedling picture;
Fig. 2 is differentiation bud picture;
Fig. 3 is the indefinite bud picture;
Fig. 4 offspring picture of making a living.
The practical implementation method
Following examples are described further the present invention.
Embodiment 1
Step 1, seed treatment: the sweet angle seed that will select is removed pulp, and water is rinsed well, is placed on refrigeration taking-up after 15 days in 3～6 ℃ of refrigerating chambers.
[0023]Step 2; Seed sterilization: the seed after choosing washed, refrigerates washes 3h with running water, soaks 2min with the alcoholic solution of 75% volume fraction, takes out afterwards with aseptic water washing 3 times; Soak 10min with the mercuric chloride solution of 0.1% mass fraction again, take out the back with aseptic water washing 5 times.
Step 3, aseptic seedling is cultivated: the seed after will sterilizing is inoculated in the aseptic seedling medium, and nutrient media components is MS minimal medium+6-BA0.8mg/L+NAA0.1 mg/L; Condition of culture is: 23 ± 1 ℃ of temperature; Light application time 13h/d, intensity of illumination 2500Lux turns out aseptic seedling; Incubation time 22d, Fig. 1 are the aseptic seedling picture.
Step 4, the differentiation bud is cultivated: the aseptic seedling growth was got the fritter that cotyledon is cut into 0.3cm after 5 days; Be seeded on callus induction-differential medium, nutrient media components is MS minimal medium+6-BA7mg/L+ Coconut Juice 18ml/L, the same step 3 of condition of culture; Induce and produce callus and differentiate the differentiation bud; Incubation time 22d, every callus obtains 2 differentiation buds, and Fig. 2 is differentiation bud picture.
Step 5, indefinite bud is cultivated: will break up bud and change proliferated culture medium over to, nutrient media components is MS minimal medium+6-BA0.8mg/L+IBA0.3mg/L+ active carbon 0.3g/L; Carrying out indefinite bud cultivates; The same step 3 of condition of culture, incubation time 45d, Fig. 3 are the indefinite bud picture.
Step 6, culture of rootage: change indefinite bud over to root media, nutrient media components is 1/2MS+NAA0.3mg/L+ active carbon 0.5g/L, carries out culture of rootage, the same step 3 of condition of culture, incubation time 31d obtains the seedling of taking root, Fig. 4 offspring picture of making a living.
Step 7, refining seedling, transplanting: the seedling of will taking root is opened bottleneck in the greenhouse, and it is contacted with air; Keep 22 ± 2 ℃ of humidity 80～85%, temperature, refining seedling time 3d is then with the take root medium of seedling base portion of flowing water flush away; Take out after soaking 1min with the potassium permanganate of 0.1% mass fraction, be transplanted in the cultivation matrix of sterilization and cultivate, cultivation matrix is peat soil: vermiculite=2:1; Obtain regeneration plant, regeneration plant survival rate 90%, 79 days from indefinite bud to the regeneration plant time spent.
Embodiment 2
Step 1, seed treatment: the sweet angle seed that will select is removed pulp, and water is rinsed well, is placed on refrigeration taking-up after 15 days in 3～6 ℃ of refrigerating chambers.
Step 2; Seed sterilization: the seed after choosing washed, refrigerates washes 4h with running water, soaks 3min with the alcoholic solution of 75% volume fraction, takes out afterwards with aseptic water washing 5 times; Soak 15min with the mercuric chloride solution of 0.1% mass fraction again, take out the back with aseptic water washing 5 times.
Step 3; Aseptic seedling is cultivated: the seed after will sterilizing is inoculated in the aseptic seedling medium, and nutrient media components is MS minimal medium+6-BA1.2mg/L+NAA0.5 mg/L, and condition of culture is: 25 ± 1 ℃ of temperature; Light application time 11h/d; Intensity of illumination 2500Lux turns out aseptic seedling, incubation time 23d.
Step 4, the differentiation bud is cultivated: the aseptic seedling growth was got the fritter that cotyledon is cut into 0.5cm after 4 days; Be seeded on callus induction-differential medium; Nutrient media components is MS minimal medium+6-BA10 mg/L+ Coconut Juice 22ml/L, and the same step 3 of condition of culture induces to produce callus and differentiate the differentiation bud; Incubation time 25d, every callus obtains 3 differentiation buds.
Step 5, indefinite bud is cultivated: will break up bud and change proliferated culture medium over to, nutrient media components is MS minimal medium+6-BA1.2mg/L+IBA0.8mg/L+ active carbon 0.5g/L; Carry out indefinite bud and cultivate the same step 3 of condition of culture, incubation time 40d; Select bigger indefinite bud and be used for culture of rootage; Less indefinite bud is cut into the segment of 1-2 joint, is inoculated in proliferated culture medium once more, continues to cultivate indefinite bud.
Step 6, culture of rootage: will select bigger indefinite bud and change root media over to, nutrient media components is 1/2MS+NAA0.5mg/L+ active carbon 0.3g/L, carries out culture of rootage, the same step 3 of condition of culture, incubation time is 32d, obtains the seedling of taking root.
Step 7, refining seedling, transplanting: the seedling of will taking root is opened bottleneck in the greenhouse, and it is contacted with air; Keep 22 ± 2 ℃ of humidity 85～90%, temperature, refining seedling time 2d is then with the take root medium of seedling base portion of flowing water flush away; Take out after soaking 2min with the potassium permanganate of 0.1% mass fraction, be transplanted in the cultivation matrix of sterilization and cultivate, cultivation matrix is peat soil: vermiculite=2:1; Obtain regeneration plant, regeneration plant survival rate 92%, 74 days from indefinite bud to the regeneration plant time spent.
Embodiment 3
Step 1, seed treatment: the sweet angle seed that will select is removed pulp, and water is rinsed well, is placed on refrigeration taking-up after 15 days in 3～6 ℃ of refrigerating chambers.
Step 2; Seed sterilization: the seed after choosing washed, refrigerates washes 4h with running water, and the volume fraction alcoholic solution with 75% soaks 3min, takes out afterwards with aseptic water washing 5 times; Soak 12min with the mercuric chloride solution of 0.1% mass fraction again, take out the back with aseptic water washing 5 times.
Step 3; Aseptic seedling is cultivated: the seed after will sterilizing is inoculated in the aseptic seedling medium, and nutrient media components is MS minimal medium+6-BA1.0mg/L+NAA0.4mg/L, and condition of culture is: 24 ± 1 ℃ of temperature; Light application time 12h/d; Intensity of illumination 2500Lux turns out aseptic seedling, incubation time 20d.
Step 4, the differentiation bud is cultivated: the aseptic seedling growth was got the fritter that cotyledon is cut into 0.4cm after 3 days; Be seeded on callus induction-differential medium; Nutrient media components is MS minimal medium+6-BA9 mg/L+ Coconut Juice 20ml/L, and the same step 3 of condition of culture induces to produce callus and differentiate the differentiation bud; Time 20d, every callus obtains 4 differentiation buds.
Step 5, indefinite bud is cultivated: will break up bud and change proliferated culture medium over to, nutrient media components is MS minimal medium+6-BA1.0mg/L+IBA0.5mg/L+ active carbon 0.5g/L; Carry out indefinite bud and cultivate the same step 3 of condition of culture, incubation time 35d; Select bigger indefinite bud and be used for culture of rootage; Less indefinite bud is cut into the segment of 1-2 joint, is inoculated in proliferated culture medium once more, continues to cultivate indefinite bud.
Step 6, culture of rootage: will select bigger indefinite bud and change root media over to, nutrient media components is 1/2MS+NAA0.4mg/L+ active carbon 0.5g/L, carries out culture of rootage, the same step 3 of condition of culture, incubation time 28d obtains the seedling of taking root.
Step 7, refining seedling, transplanting: the seedling of will taking root is opened bottleneck in the greenhouse, and it is contacted with air; Keep 22 ± 2 ℃ of humidity 83～88%, temperature, refining seedling time 2d is then with the take root medium of seedling base portion of flowing water flush away; Take out after soaking 2min with the potassium permanganate of 0.1% volumetric concentration, be transplanted in the cultivation matrix of sterilization and cultivate, cultivation matrix is peat soil: vermiculite=2:1; Obtain regeneration plant, regeneration plant survival rate 93%, 65 days from indefinite bud to the regeneration plant time spent.
Claims (7)
1. sweet angle method for quickly breeding, it is characterized in that: a kind of sweet angle method for quickly breeding is characterized in that: the sweet angle seed refrigeration sterilization treatment that will select wash clean earlier; Then seed is inoculated in the aseptic seedling medium, nutrient media components is: MS minimal medium+6-BA 0.8～1.2mg/L+NAA0.1～0.5mg/L, cultivate aseptic seedling; The cotyledon stripping and slicing youngster of aseptic seedling is inoculated into callus induction-differential medium, and nutrient media components is MS minimal medium+6-BA7～10mg/L+ Coconut Juice 18～22ml/L, induces to produce callus and differentiate the differentiation bud; The differentiation bud that obtains changes proliferated culture medium over to; Nutrient media components is MS minimal medium+6-BA0.8～1.2mg/L+IBA0.3～0.8mg/L+ active carbon 0.3～0.5g/L, turns out indefinite bud, and less indefinite bud is cut into the segment of 1-2 joint; Be inoculated in proliferated culture medium once more; Continue the Culture and Differentiation indefinite bud, bigger indefinite bud inserts root media, and nutrient media components is 1/2MS+ NAA0.3～0.5mg/L+ active carbon 0.3～0.5g/L; Culture of rootage obtains the seedling of taking root, and the seedling of taking root forms regeneration plant through refining seedling, transplanting.
2. a kind of sweet angle as claimed in claim 1 method for quickly breeding is characterized in that: the refrigeration of said sweet angle seed is treated in 3～6 ℃ of refrigerating chambers and refrigerates 15 days.
3. a kind of sweet angle as claimed in claim 1 method for quickly breeding; It is characterized in that: said sweet angle seed sterilizing methods is: the seed after choosing is washed, refrigerated is with running water flushing 3～4h; Alcoholic solution with 75% volume fraction soaks 2～3min; Take out the back with aseptic water washing 3～5 times, the mercuric chloride solution with 0.1% mass fraction soaks 10～15min again, after the taking-up with aseptic water washing 5 times.
4. a kind of sweet angle as claimed in claim 1 method for quickly breeding; It is characterized in that: said aseptic seedling is cultivated, the differentiation bud is cultivated, indefinite bud is cultivated identical with the condition of culture of culture of rootage: 23～26 ℃ of cultivation temperature; Light application time 11～13h/d, intensity of illumination 2500Lux.
5. a kind of sweet angle as claimed in claim 1 method for quickly breeding, it is characterized in that: the aseptic seedling incubation time is 20-23d, and differentiation bud incubation time is 20-25d, and the indefinite bud incubation time is 35-45d, and the culture of rootage time is 28-32d, refining seedling time 2-3d.
6. a kind of sweet angle as claimed in claim 1 method for quickly breeding is characterized in that: the method for said refining seedling, the transplanting seedling bottleneck of be-will taking root is opened, and it is contacted with air; Keep 22 ± 2 ℃ of humidity 80～90%, temperature; Refining seedling time 2-3d with the take root medium of seedling base portion of flowing water flush away, takes out behind the liquor potassic permanganate immersion 1～2min with 0.1% mass fraction then; Be transplanted in the cultivation matrix of sterilization and cultivate, obtain regeneration plant.
7. a kind of sweet angle as claimed in claim 6 method for quickly breeding, it is characterized in that: said cultivation matrix is peat soil: vermiculite=2:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110241503 CN102405834B (en) | 2011-08-22 | 2011-08-22 | Rapid propagation method of Tianjiao |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110241503 CN102405834B (en) | 2011-08-22 | 2011-08-22 | Rapid propagation method of Tianjiao |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102405834A true CN102405834A (en) | 2012-04-11 |
| CN102405834B CN102405834B (en) | 2013-01-30 |
Family
ID=45908403
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110241503 Expired - Fee Related CN102405834B (en) | 2011-08-22 | 2011-08-22 | Rapid propagation method of Tianjiao |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102405834B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106234226A (en) * | 2016-08-23 | 2016-12-21 | 中央民族大学 | A kind of in-vitro culture method of Ligularia rumicifolia (Drumm.) S.W. Liu. |
-
2011
- 2011-08-22 CN CN 201110241503 patent/CN102405834B/en not_active Expired - Fee Related
Non-Patent Citations (6)
| Title |
|---|
| 《CURRENT SCIENCE》 20000525 Urmil J. Mehta等 "Regeneration of plants via adventitious bud formation from mature zygotic embryo axis of tamarind (Tamarindus indica L.)" 第1231-1234页 1-7 第78卷, 第10期 * |
| 《Plant Cell Reports》 19911231 Pawan K. Jaiwal等 "In vitro high frequency plant regeneration of a tree legume Tamarindus indica (L.)" 第569-573页 1-7 第10卷, 第11期 * |
| 《云南农业大学学报》 20050228 赵一鹤等 "世界酸角研究现状及进展" 第65-72页 1-7 第20卷, 第1期 * |
| PAWAN K. JAIWAL等: ""In vitro high frequency plant regeneration of a tree legume Tamarindus indica (L.)"", 《PLANT CELL REPORTS》 * |
| URMIL J. MEHTA等: ""Regeneration of plants via adventitious bud formation from mature zygotic embryo axis of tamarind (Tamarindus indica L.)"", 《CURRENT SCIENCE》 * |
| 赵一鹤等: ""世界酸角研究现状及进展"", 《云南农业大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106234226A (en) * | 2016-08-23 | 2016-12-21 | 中央民族大学 | A kind of in-vitro culture method of Ligularia rumicifolia (Drumm.) S.W. Liu. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102405834B (en) | 2013-01-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101926287B (en) | Method for culturing tissue of 'Zhongzhen No.1' | |
| CN101647392B (en) | Tissue-culture rapid-propagation method of double-happiness cuckoo variety and special culture medium thereof | |
| CN104186190B (en) | Fruit tree hybrid seed sprouting and rooting method | |
| CN101584297B (en) | Tissue culture and regenerated plant in vitro mycorrhization method for pinus massoniana | |
| CN101595824B (en) | Rapid in-vitro seedling raising method by utilizing sandalwood seed embryo | |
| CN102487816A (en) | Wheat double haploid production method by wheat-and-maize distant hybridization | |
| CN102090341B (en) | Method for rapidly breeding jewel orchid | |
| CN104145818A (en) | Preservation method of gerbera germplasm resources | |
| CN1284448C (en) | High quality germchit tissure culturing and rapid breeding method of Dendrobium sp. | |
| CN105918129B (en) | A kind of tissue culture and rapid propagation method of moonlight jujube | |
| Mumo et al. | In vitro regeneration of selected Kenyan papaya (Carica papaya L.) lines through shoot tip culture | |
| CN110150147B (en) | Tissue culture and rapid propagation method for populus euphratica | |
| CN103109747B (en) | Rapid pseudolarix propagation method based on stem node propagation | |
| CN103109728B (en) | Rapid seedling culturing method of pinus sylvestris in tube | |
| CN108575746A (en) | A kind of Chinese herbaceous peony vitro Regeneration System method for building up | |
| CN107278566A (en) | A kind of tomato lateral branch cutting fast breeding method | |
| CN106857258A (en) | A kind of quick breeding method for tissue culture blue with leaf pocket | |
| Mišković et al. | Effect of different rootstock type on quality and yield of tomato fruits | |
| CN110199884A (en) | A kind of breeding method of setting seeds under quinoa tissue-cultured seedling aseptic condition | |
| CN102405834B (en) | Rapid propagation method of Tianjiao | |
| CN111202002B (en) | Tissue culture and rapid propagation method of clerodendrum japonicum | |
| CN103828710A (en) | Efficient chrysanthemum cross-breeding method | |
| CN102763598B (en) | Method for breeding wenshan paphiopedilum seedlings by using somatic embryo | |
| CN106688767A (en) | Method for raising Jiangnan keteleeria fortunei seedlings | |
| CN112616663A (en) | Method for greatly shortening planting period of lilium davidii var davidii and rapidly propagating seedlings |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130130 Termination date: 20130822 |