CN102399697A - Nutrient solution for microalgae cultivation - Google Patents
Nutrient solution for microalgae cultivation Download PDFInfo
- Publication number
- CN102399697A CN102399697A CN2011103872959A CN201110387295A CN102399697A CN 102399697 A CN102399697 A CN 102399697A CN 2011103872959 A CN2011103872959 A CN 2011103872959A CN 201110387295 A CN201110387295 A CN 201110387295A CN 102399697 A CN102399697 A CN 102399697A
- Authority
- CN
- China
- Prior art keywords
- concentration
- algae
- growth
- kno
- feso
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000015097 nutrients Nutrition 0.000 title description 3
- 239000007853 buffer solution Substances 0.000 claims abstract description 19
- 230000000050 nutritive effect Effects 0.000 claims description 21
- 238000012258 culturing Methods 0.000 claims description 15
- 241000195493 Cryptophyta Species 0.000 abstract description 39
- 230000012010 growth Effects 0.000 abstract description 17
- 241000894007 species Species 0.000 abstract description 4
- 239000000243 solution Substances 0.000 abstract description 3
- 239000003225 biodiesel Substances 0.000 abstract description 2
- 239000007836 KH2PO4 Substances 0.000 abstract 1
- 229910017677 NH4H2 Inorganic materials 0.000 abstract 1
- 238000010353 genetic engineering Methods 0.000 abstract 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 abstract 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 abstract 1
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Inorganic materials [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 20
- 229910052757 nitrogen Inorganic materials 0.000 description 10
- 229910052799 carbon Inorganic materials 0.000 description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 230000002860 competitive effect Effects 0.000 description 4
- 150000002632 lipids Chemical class 0.000 description 4
- 230000029553 photosynthesis Effects 0.000 description 4
- 238000010672 photosynthesis Methods 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 241000192700 Cyanobacteria Species 0.000 description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 3
- 235000011089 carbon dioxide Nutrition 0.000 description 3
- 230000019522 cellular metabolic process Effects 0.000 description 3
- 239000004519 grease Substances 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229910052710 silicon Inorganic materials 0.000 description 3
- 239000010703 silicon Substances 0.000 description 3
- 108010027322 single cell proteins Proteins 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 239000002551 biofuel Substances 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 230000000243 photosynthetic effect Effects 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 1
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241000124105 Isochrysis sp. Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- 241000159660 Nannochloropsis oculata Species 0.000 description 1
- 241000180701 Nitzschia <flatworm> Species 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 229930003756 Vitamin B7 Natural products 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- ASARMUCNOOHMLO-WLORSUFZSA-L cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2s)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@H](C)OP([O-])(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O ASARMUCNOOHMLO-WLORSUFZSA-L 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000003317 industrial substance Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 1
- 235000008160 pyridoxine Nutrition 0.000 description 1
- 239000011677 pyridoxine Substances 0.000 description 1
- NHDHVHZZCFYRSB-UHFFFAOYSA-N pyriproxyfen Chemical compound C=1C=CC=NC=1OC(C)COC(C=C1)=CC=C1OC1=CC=CC=C1 NHDHVHZZCFYRSB-UHFFFAOYSA-N 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000011912 vitamin B7 Nutrition 0.000 description 1
- 239000011735 vitamin B7 Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Microalgae used for extracting algal oil and preparing biodiesel have special properties. Generally, the growth is required to be rapid (the period is not more than 20 days), the oil content is high (not less than 15 percent, dry weight), and the growth advantage can be achieved in an open system. The current genetic engineering can not completely obtain the algae species meeting the requirements. Especially in open systems, has not been possible under the current technical conditions. The advantageous growth of the desired algal species can be achieved only by a semi-open system, i.e. by creating an ecological environment by limiting the culture conditions, even when other species are introduced. The invention realizes the dominant growth of the required algae species in a semi-open system through the design of the culture solution. Specifically, Ca (NO)3)2Concentration of 0.4-1g/L, KNO3The concentration is 0.4-1g/L KH2PO4Concentration of 0.4-1g/L, MgSO4The concentration is 0.2-0.6g/L, FeSO4The concentration is 0.1-0.5g/L, and the buffer system is (NH)4）2HPO4/NH4H2PO4The concentration is 2-3 g/L.
Description
Technical field
The invention belongs to biological chemical field, be specially the design of the grown cultures system of a kind of little algae, comprise the composition of nutrient solution, and corresponding external environment condition.
Background technology
Little algae be one type on land, the ocean distributes extensively; The high holophyte of nutritious, photosynthetic availability; The polysaccharide that cellular metabolism produces, protein, pigment etc. make it have good DEVELOPMENT PROSPECT in fields such as food, medicine, genetically engineered, liquid fuels.Little algae is of a great variety, contains in the microalgae cell: the nutritive ingredient and the industrial chemicals of protein, lipid, polysaccharides, β-Hu Luobusu, multiple inorganic elements high values such as (like Cu, Fe, Se, Mn, Zn etc.).The protein contnt of little algae is very high, is an important source of single cell protein (SCP).Contained vitamin A, vitamin E, thiamine, vitamin G, pyridoxol, cobalamin, vitamins C, vitamin H, inositol, folic acid, VA and the nicotinic acid etc. of little algae have increased its value as SCP.
Little algae be the most simply, one of the most ancient lower plant; Not only kind is many, distribution is wide, breeding is fast; And photosynthetic efficiency is high; It can directly utilize simple nutritive substance such as sunlight, carbonic acid gas, nitrogen, phosphorus to grow fast and in born of the same parents, synthesize a large amount of greases (like triglyceride level), for production of biodiesel provides new oil resource.
Nutritive salt in the substratum has very big influence for algal grown.The nutritive salt shortage can cause cellular metabolism and photosynthesis intermediate product unusual.Nutritive salt comprises trace elements such as mineral ions such as principal elements such as nitrogen, phosphorus, iron, silicon, magnesium, sodium and copper, manganese, zinc, molybdenum, cobalt and VITAMINs.Nitrogen has accounted for the 7-10 % of dry weight in the frond, and nitrogen is the essential composition that constitutes important compound such as protein, amino acid, ferment, co-ferment, chlorophyll and cytolemma, in cellular metabolism, plays the part of important role.C/N ratio (C/N ratio) can influence the algae fat content.In higher C/N ratio, that is under the environment of excessive carbon source and insufficient nitrogen, can make algae accumulate grease.Yet some algae but is the accumulation glucide under the environment of insufficient nitrogen.C/N ratio also can influence the microalgae grease kind.Constitute by specific composition in some alginite; Form by silicon mostly like diatom; Therefore in substratum, adding enough silicon is that (the salinity height can influence the intravital metabolism situation of unicellular algae to necessary condition; This is because salinity can influence the osmotic pressure of nutrient solution, and too high osmotic pressure can suppress the carrying out of lightsystem in the photosynthesis (PS II), and then suppresses photosynthesis.Salinity is formed all influential for algal grown, fat content, lipid acid, look the algae kind and different.When salinity increased, the fat content of Isochrysis sp. and Nannochloropsis oculata can rise, and Nitzschia is then opposite.The influence of many research and inquirement salinity for algae EPA and DHA content also arranged.Salinity also can influence the carotenoid content of algae.Some algae is high for the salinity tolerance level of growing environment, and then its growth, cell are formed not change with salinity has too about-face.
In the process of algae culture, the consumption of carbonic acid gas is the principal element that causes the pH value to change, and the pH value that degraded caused of the consumption of nutritive salt or metabolite changes then can be ignored.The carbonic acid gas that is dissolved in the water can be reached balance with carbonate and hydrocarbonate.
Because algal grown meeting absorbing carbon dioxide, according to reining in Saudi Arabia's row law, balance can cause [H+] to reduce toward moving to left, and the pH value rises, and this situation is more obvious under high-density culture.The variation of pH value can influence the solubleness of inorganics in the substratum, and then influences the metabolism of algae.Each algae kind has the pH value of growing that is fit to of difference, adds buffered soln, with control pH value, can make algae that preferable growth performance is arranged.Except the above-mentioned cultivation factor of introducing, incubation time also can influence algal grown, and after getting into lag phase, algae begins to accumulate grease along with the time increase.Disturbance also can influence algal grown, as increasing disturbance, can make alga cells evenly receive light, the oxygen that also removable photosynthesis produced.
Summary of the invention
1, technical problem to be solved:
Put the both culturing microalgae under the system condition at semi-open even standard-sized sheet, the artificial environment that is the basis with the prescription of nutritive medium.Under this environment, little algae can realize rapidly and efficiently growing, even having under the competitive environment of alien species, still can keep enough competitive edges, thereby is implemented in the dominant growth under the semi-open environment.
2, technical scheme:
The invention provides a kind of little algae and rapidly and efficiently culture and use nutritive medium, it is characterized in that: be grouped into Ca (NO by following one-tenth
3)
2, concentration is 0.4-1g/L, KNO
3,Concentration is 0.4-1g/L KH
2PO
4, concentration is 0.4-1g/L, MgSO
4, concentration is 0.2-0.6g/L, FeSO
4, concentration is 0.1-0.5g/L.
Also have buffer system, buffer system is (NH
4)
2HPO
4/ NH
4H
2PO
4, concentration is 2-3g/L.
3, beneficial effect:
The little algae that the present invention relates to is green alga and blue-green algae.These 2 types of little algaes have similar structure to environment requirement.In the process of both culturing microalgae production biofuel, hope that little algae builds up fat rather than glucide in process of growth, i.e. the both culturing microalgae of high lipid content.Therefore, consciously in Environment Design increase substantially the nitrogen content in the nutritive medium and reduce the supply of carbon, thereby cause the fat accumulation of little algae.Most of little algae of it should be noted that occurring in nature all is to be main energy derive with carbon, although under high nitrogen low-carbon (LC) environment, also can survive, descending significantly all appears in the speed of growth and reproduction speed.And 2 types of algae kinds of the present invention's design can still keep greater efficiency under this environment growth and procreation, thereby realize advantage competition.
Embodiment
In order to be implemented in semi-open even standard-sized sheet is put the both culturing microalgae under the system condition, the present invention has designed a special water surrounding.It mainly is the artificial environment that the prescription with nutritive medium is the basis.Under this environment, little algae can realize rapidly and efficiently growing, even having under the competitive environment of alien species, still can keep enough competitive edges, thereby is implemented in the dominant growth under the semi-open environment.The little algae that the present invention relates to is green alga and blue-green algae.These 2 types of little algaes have similar structure to environment requirement.In the process of both culturing microalgae production biofuel, hope that little algae builds up fat rather than glucide in process of growth, i.e. the both culturing microalgae of high lipid content.Therefore, consciously in Environment Design increase substantially the nitrogen content in the nutritive medium and reduce the supply of carbon, thereby cause the fat accumulation of little algae.Most of little algae of it should be noted that occurring in nature all is to be main energy derive with carbon, although under high nitrogen low-carbon (LC) environment, also can survive, descending significantly all appears in the speed of growth and reproduction speed.And 2 types of algae kinds of the present invention's design can still keep greater efficiency under this environment growth and procreation, thereby realize advantage competition.In addition, through regulating, required algae kind dominant growth and the purpose that suppresses external algae kind growth have also been realized promoting to a certain extent to pH value.The suitable pH value of blue-green algae and green alga is between the 7-9.Setting through buffer system can be that environment remains under this PH, and little algae of those growths under high alkalinity environment more that suit just is difficult to competition existence.
According to above-mentioned design, adopt nutritive medium fully as follows: Ca (NO
3)
2, concentration is 0.4-1g/L, KNO
3,Concentration is 0.4-1g/L KH
2PO
4, concentration is 0.4-1g/L, MgSO
4, concentration is 0.2-0.6g/L, FeSO
4, concentration is 0.1-0.5g/L, buffer system is (NH
4)
2HPO
4/ NH
4H
2PO
4, concentration is 2-3g/L.The pH value of whole system is stabilized between the 7-9.
Specific embodiments:
1:Ca (NO
3)
2, concentration is 0.4g/L, KNO
3,Concentration is 0.4g/L KH
2PO
4, concentration is 0.4g/L, MgSO
4, concentration is 0.2g/L, FeSO
4, concentration is 0.1g/L, buffer system is (NH
4)
2HPO
4/ NH
4H
2PO
4, concentration is 2g/L.
2:Ca (NO
3)
2, concentration is 0.6g/L, KNO
3,Concentration is 0.8g/L KH
2PO
4, concentration is 0.8g/L, MgSO
4, concentration is 0.4g/L, FeSO
4, concentration is 0.3g/L, buffer system is (NH
4)
2HPO
4/ NH
4H
2PO
4, concentration is 2g/L.
3:Ca (NO
3)
2, concentration is 1g/L, KNO
3,Concentration is 1g/L KH
2PO
4, concentration is 1g/L, MgSO
4, concentration is 0.6g/L, FeSO
4, concentration is 0.5g/L, buffer system is (NH
4)
2HPO
4/ NH
4H
2PO
4, concentration is 2g/L.
4:Ca (NO
3)
2, concentration is 0.4g/L, KNO
3,Concentration is 0.4g/L KH
2PO
4, concentration is 0.4g/L, MgSO
4, concentration is 0.2g/L, FeSO
4, concentration is 0.1g/L, buffer system is (NH
4)
2HPO
4/ NH
4H
2PO
4, concentration is 3g/L.
5:Ca (NO
3)
2, concentration is 0.6g/L, KNO
3,Concentration is 0.8g/L KH
2PO
4, concentration is 0.8g/L, MgSO
4, concentration is 0.4g/L, FeSO
4, concentration is 0.3g/L, buffer system is (NH
4)
2HPO
4/ NH
4H
2PO
4, concentration is 3g/L.
6:Ca (NO
3)
2, concentration is 1g/L, KNO
3,Concentration is 1g/L KH
2PO
4, concentration is 1g/L, MgSO
4, concentration is 0.6g/L, FeSO
4, concentration is 0.5g/L, buffer system is (NH
4)
2HPO
4/ NH
4H
2PO
4, concentration is 3g/L.
Though the present invention with preferred embodiment openly as above; But they be not be used for limiting of the present invention; Anyly have the knack of this art; In spirit that does not break away from the present invention and scope, certainly when can doing various variations or retouching, so being as the criterion of should being defined with the application's claim protection domain of protection scope of the present invention.
Claims (8)
1. a both culturing microalgae is used nutritive medium, it is characterized in that: be grouped into Ca (NO by following one-tenth
3)
2, concentration is 0.4-1g/L, KNO
3,Concentration is 0.4-1g/L KH
2PO
4, concentration is 0.4-1g/L, MgSO
4, concentration is 0.2-0.6g/L, FeSO
4, concentration is 0.1-0.5g/L.
2. both culturing microalgae as claimed in claim 1 is used nutritive medium, it is characterized in that: Ca (NO
3)
2, concentration is 0.4g/L, KNO
3,Concentration is 0.4g/L KH
2PO
4, concentration is 0.4g/L, MgSO
4, concentration is 0.2g/L, FeSO
4, concentration is 0.1g/L, buffer system is (NH
4)
2HPO
4/ NH
4H
2PO
4, concentration is 2g/L.
3. both culturing microalgae as claimed in claim 1 is used nutritive medium, it is characterized in that: Ca (NO
3)
2, concentration is 0.6g/L, KNO
3,Concentration is 0.8g/L KH
2PO
4, concentration is 0.8g/L, MgSO
4, concentration is 0.4g/L, FeSO
4, concentration is 0.3g/L, buffer system is (NH
4)
2HPO
4/ NH
4H
2PO
4, concentration is 2g/L.
4. both culturing microalgae as claimed in claim 1 is used nutritive medium, it is characterized in that: Ca (NO
3)
2, concentration is 1g/L, KNO
3,Concentration is 1g/L KH
2PO
4, concentration is 1g/L, MgSO
4, concentration is 0.6g/L, FeSO
4, concentration is 0.5g/L, buffer system is (NH
4)
2HPO
4/ NH
4H
2PO
4, concentration is 2g/L.
5. both culturing microalgae as claimed in claim 1 is used nutritive medium, it is characterized in that: Ca (NO
3)
2, concentration is 0.4g/L, KNO
3,Concentration is 0.4g/L KH
2PO
4, concentration is 0.4g/L, MgSO
4, concentration is 0.2g/L, FeSO
4, concentration is 0.1g/L, buffer system is (NH
4)
2HPO
4/ NH
4H
2PO
4, concentration is 3g/L.
6. both culturing microalgae as claimed in claim 1 is used nutritive medium, it is characterized in that: Ca (NO
3)
2, concentration is 0.6g/L, KNO
3,Concentration is 0.8g/L KH
2PO
4, concentration is 0.8g/L, MgSO
4, concentration is 0.4g/L, FeSO
4, concentration is 0.3g/L, buffer system is (NH
4)
2HPO
4/ NH
4H
2PO
4, concentration is 3g/L.
7. both culturing microalgae as claimed in claim 1 is used nutritive medium, it is characterized in that: Ca (NO
3)
2, concentration is 1g/L, KNO
3,Concentration is 1g/L KH
2PO
4, concentration is 1g/L, MgSO
4, concentration is 0.6g/L, FeSO
4, concentration is 0.5g/L, buffer system is (NH
4)
2HPO
4/ NH
4H
2PO
4, concentration is 3g/L.
8. use nutritive medium like the described both culturing microalgae of the arbitrary claim of claim 1-7, it is characterized in that: also have buffer system, buffer system is (NH
4)
2HPO
4/ NH
4H
2PO
4, concentration is 2-3g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103872959A CN102399697A (en) | 2011-11-29 | 2011-11-29 | Nutrient solution for microalgae cultivation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103872959A CN102399697A (en) | 2011-11-29 | 2011-11-29 | Nutrient solution for microalgae cultivation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102399697A true CN102399697A (en) | 2012-04-04 |
Family
ID=45882353
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011103872959A Pending CN102399697A (en) | 2011-11-29 | 2011-11-29 | Nutrient solution for microalgae cultivation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102399697A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9738869B2 (en) | 2013-05-29 | 2017-08-22 | Centre De Recherche Industrielle Du Quebec | Method and system for the culture of microalgae |
-
2011
- 2011-11-29 CN CN2011103872959A patent/CN102399697A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9738869B2 (en) | 2013-05-29 | 2017-08-22 | Centre De Recherche Industrielle Du Quebec | Method and system for the culture of microalgae |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Barghbani et al. | Investigating the effects of several parameters on the growth of Chlorella vulgaris using Taguchi's experimental approach | |
| Markou et al. | Ammonia inhibition on Arthrospira platensis in relation to the initial biomass density and pH | |
| CN101575584B (en) | Vibrio schizogenum and method for producing DHA grease by using same | |
| US8173391B2 (en) | Golden yellow algae and method of producing the same | |
| KR20160091905A (en) | Process for enrichment of microalgal biomass with carotenoids and with proteins | |
| WO2012071983A1 (en) | Heterotrophic culturing method for microalgae with high yields | |
| KR101694711B1 (en) | Culturing method of microalgae for increasing lipid productivity | |
| CN103352006A (en) | Culture method for promoting autotrophy microalgae neutral lipid accumulation | |
| CN101148643A (en) | Producing method of rhodotorula mucilaginosa for holothurian | |
| CN103045478B (en) | Method for culturing oil-producing microalgae with high density and high lipid production rate | |
| CN106566775B (en) | Preparation method of high-activity haematococcus pluvialis cells | |
| CN101063085A (en) | Method for black moss cell high-density culture of stable high-yield black moss polysaccharide | |
| CN100400643C (en) | Method for culturing crescent rhomboidal algae and its special culture medium | |
| CN102071143A (en) | Culture method for efficiently inducing accumulation of nannochloropsis oculata fat | |
| CN100400642C (en) | Traingular brown algae open culture method and its special culture meidum | |
| CN106520559A (en) | High-efficiency light autotrophic culture method for chlorella | |
| CN106480155B (en) | Method suitable for promoting haematococcus pluvialis to produce astaxanthin under high-temperature condition | |
| KR20120110295A (en) | Composition of culture medium for algae and method of culturing algae | |
| CN102399697A (en) | Nutrient solution for microalgae cultivation | |
| KR101797070B1 (en) | Culture compositions for promoting growth of spirulina and the method culturing for promoting growth of spirulina by using the same | |
| Okauchi et al. | Optimum medium for large-scale culture of Tetraselmis tetrathele | |
| Imamoglu et al. | Semi-continuous cultivation of Haematococcus pluvialis for commercial production | |
| KR102134885B1 (en) | A high efficiency system for continuous culture of microalgae | |
| KR100808115B1 (en) | The method of preparing spirulina medium using deep water | |
| CN106754385A (en) | A kind of utilization blue-green alga bloom is the method for raw material cultivation Chlorella phytoplankton |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120404 |