CN102393360A - Method for measuring optical rotation of L-tartaric acid - Google Patents
Method for measuring optical rotation of L-tartaric acid Download PDFInfo
- Publication number
- CN102393360A CN102393360A CN2011102516887A CN201110251688A CN102393360A CN 102393360 A CN102393360 A CN 102393360A CN 2011102516887 A CN2011102516887 A CN 2011102516887A CN 201110251688 A CN201110251688 A CN 201110251688A CN 102393360 A CN102393360 A CN 102393360A
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- Prior art keywords
- tartaric acid
- tartrate
- polarimeter
- measuring
- repeatedly
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- 230000003287 optical effect Effects 0.000 title claims abstract description 32
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 16
- 239000012153 distilled water Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000001816 cooling Methods 0.000 claims abstract description 8
- 238000005303 weighing Methods 0.000 claims abstract description 7
- 238000001035 drying Methods 0.000 claims abstract description 6
- FEWJPZIEWOKRBE-JCYAYHJZSA-L L-tartrate(2-) Chemical compound [O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O FEWJPZIEWOKRBE-JCYAYHJZSA-L 0.000 claims description 20
- 230000010287 polarization Effects 0.000 claims description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 239000013543 active substance Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention provides a method for measuring the optical rotation of L-tartaric acid, which comprises the following steps of: A. feeding a certain amount of L-tartaric acid in a baking oven for drying, then taking out and feeding the dried L-tartaric acid in a drying box for cooling; after cooling, weighing a sample and repeatedly drying, cooling and weighing the L-tartaric acid until the weight of the L-tartaric acid changes no longer; B. weighing 10g of the L-tartaric acid obtained in the step A, dissolving the L-tartaric acid into a 100ml volumetric flask with distilled water and measuring the volume of the L-tartaric acid to be 100ml; C. carrying out a blank test with the distilled water and calibrating a polarimeter; and D. repeatedly rinsing the polarimeter with the prepared L-tartaric acid solution for multiple times; and after rinsing, pouring the prepared L-tartaric acid solution into the polarimeter for measuring to obtain the optical rotation. The method has the beneficial effect that the measuring precision of the optical rotation of the L-tartaric acid is improved by using added limited steps.
Description
Technical field
the present invention relates to physico-chemical analysis field, particularly a kind of measuring method of L-tartrate optical activity.
Background technology
When chiral material was passed through when a branch of single linearly polarized light in
, its direction of vibration can change, and the vibration plane in this time rotates a certain angle, and this phenomenon is called optical rotation phenomenon.This character of the vibration plane rotation of polarized light that makes of material is called optical activity, and the material with optical activity is called optically active substance or optical active substance.Many natural organic matters all have optical activity.Because optical active substance makes polarized light vibration plane when rotation, can dextrorotation (CW, note is done "+"), also can left-handed (counterclockwise, note be done "-"), so optical active substance can be divided into dextrorotatory substance and levo-rotatory substance again.
The light that
are sent by monochromatic source (generally using sodium lamp) behind polarizing prism (nicol prism), changes linearly polarized light (abbreviation polarized light) into.When polarized light passed through the optically active substance in the sample hose, the plane of oscillation rotated to an angle.Regulate the analyzer (also being a nicol prism) with scale, polarized light is passed through, the number of degrees that analyzer rotated are presented on the index dial, and this is the actual measurement optical activity α of sample.
The size of
optical activity is except the spatial structure that depends on tested molecule; Also receive the influence of the factors such as wavelength, solvent for use of thickness (being the length of sample hose) through solution of the concentration, polarized light of solution to be measured, temperature, used light source, these factors all will show in measuring the result.Specific rotatory power commonly used is represented the optical activity of material, and the relation of specific rotatory power and optical activity is following:
The specific rotatory power of neat liquid
The specific rotatory power of solution
In the above two formula, 
The expression optically active substance is t ℃ in temperature, the specific rotatory power the when wavelength of light source is λ; α is the measured optical activity of polarimeter; L is liquid layer thickness (dm); D is the density of neat liquid; C is the concentration (g/ml) of solution; Temperature when t is mensuration (℃); λ is the wavelength (nm) of used light source.For example 25 ℃ are used wavelength to make light source as the sodium vapor lamp (D line) of 589nm to measure the optical activities of certain sample and be 38 ° of dextrorotation, and then the specific rotatory power note is made [α]
=+ 38 °.
It is lower that
existing method is measured the common ratio of precision of the tartaric optical activity method of L-, has a strong impact on experimental measurements, and therefore how accurately measuring the tartaric optical activity of L-becomes this technical field problem demanding prompt solution.
Summary of the invention
Measure the lower problem of method ratio of precision of L-tartrate optical activity in order to solve existing method, the present invention proposes following technical scheme:
A kind of measuring method of L-tartrate optical activity, this method may further comprise the steps:
A, get a certain amount of L-tartrate and put into baking oven and dry, take out then, put into the drying box cooling, cooling is then weighed to sample, repeatedly L-tartrate is dried, cools off, is weighed, till weight no longer changes;
B, take by weighing the L-tartrate 10g that steps A obtains, be dissolved in the 100ml volumetric flask with distilled water, and be settled to 100ml;
C, do blank test, polarimeter is calibrated with distilled water;
The L-tartaric acid solution that
D, use prepare carries out rinse to polarization tube, and rinse is repeatedly poured the L-tartaric acid solution for preparing into after the rinse completion repeatedly, puts into polarimeter and measures, and obtains optical activity.
The beneficial effect that
the present invention brings is: the present invention is through increasing the measuring accuracy that limited step has improved L-tartrate optical activity.
Embodiment
Set forth in the face of preferred embodiment of the present invention down in detail
, thereby so that advantage of the present invention and characteristic can be easier to it will be appreciated by those skilled in the art that protection scope of the present invention is made more explicit defining.
A kind of measuring method of L-tartrate optical activity, its concrete steps are following:
A, the L-tartrate of getting 20g is put into baking oven dries, and takes out then, puts into drying box and cools off; Weigh to sample in the cooling back; Repeatedly L-tartrate is dried, cools off, weighed, till weight no longer changes, obtain dry L-tartrate sample 15.628g;
B, take by weighing the L-tartrate 10g that steps A obtains, be dissolved in the 100ml volumetric flask with distilled water, and be settled to 100ml;
C, do blank test, polarimeter is calibrated, make that the reading of polarimeter is zero when measuring distilled water with distilled water;
The L-tartaric acid solution that
D, use prepare carries out rinse to polarization tube; Rinse is 3 times repeatedly; Rinse is poured the L-tartaric acid solution for preparing into after accomplishing; Put into polarimeter and carry out measuring for 5 times optical activity respectively and be respectively 1.171,1.171,1.171,1.171,1.171, standard deviation is zero; Measure and adopt method commonly used that L-tartrate is carried out optical activity, promptly take by weighing and carry out measuring for 5 times optical activity after the sample preparation of 1g L-tartrate and be respectively 0.115,0.118,0.119,0.116,0.115, obtaining standard deviation is 0.00000241.Therefore, the inventive method has effectively improved the measuring accuracy of L-tartrate optical activity.
the above; Be merely embodiment of the present invention; But protection scope of the present invention is not limited thereto; Any those of ordinary skill in the art are in the technical scope that the present invention disclosed, and variation or the replacement that can expect without creative work all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain that claims were limited.
Claims (1)
1. the measuring method of a L-tartrate optical activity, it is characterized in that: this method may further comprise the steps:
A, get a certain amount of L-tartrate and put into baking oven and dry, take out then, put into the drying box cooling, cooling is then weighed to sample, repeatedly L-tartrate is dried, cools off, is weighed, till weight no longer changes;
B, take by weighing the L-tartrate 10g that steps A obtains, be dissolved in the 100ml volumetric flask with distilled water, and be settled to 100ml;
C, do blank test, polarimeter is calibrated with distilled water;
The L-tartaric acid solution that D, use prepare carries out rinse to polarization tube, and rinse is repeatedly poured the L-tartaric acid solution for preparing into after the rinse completion repeatedly, puts into polarimeter and measures, and obtains optical activity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102516887A CN102393360A (en) | 2011-08-30 | 2011-08-30 | Method for measuring optical rotation of L-tartaric acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102516887A CN102393360A (en) | 2011-08-30 | 2011-08-30 | Method for measuring optical rotation of L-tartaric acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102393360A true CN102393360A (en) | 2012-03-28 |
Family
ID=45860720
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011102516887A Pending CN102393360A (en) | 2011-08-30 | 2011-08-30 | Method for measuring optical rotation of L-tartaric acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102393360A (en) |
-
2011
- 2011-08-30 CN CN2011102516887A patent/CN102393360A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 王秋长 等: "《基础化学实验》", 31 March 2003, 科学出版社 * |
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| PB01 | Publication | ||
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
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Application publication date: 20120328 |