CN102370674A - Mistletoe extract, its preparation method and its application - Google Patents
Mistletoe extract, its preparation method and its application Download PDFInfo
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- CN102370674A CN102370674A CN2010102609834A CN201010260983A CN102370674A CN 102370674 A CN102370674 A CN 102370674A CN 2010102609834 A CN2010102609834 A CN 2010102609834A CN 201010260983 A CN201010260983 A CN 201010260983A CN 102370674 A CN102370674 A CN 102370674A
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Abstract
The invention belongs to the plant pharmaceutical chemistry field, and provides a mistletoe extract. The mistletoe extract takes dry stems and leaves of mistletoe as a raw material, and is obtained by extraction and purification. In the mistletoe extract, by weighting three flavonoids of avicularin, quercetin and quercitin, the weight percentage of the flavonoids is greater than 50%. The invention provides a method for preparing the mistletoe extract, and also provides an application of the mistletoe extract for preparing medicines or health foods to prevent or treat hyperuricemia or gout. Animal test results show that the mistletoe total flavonoid has substantial effect for reducing uric acid in the blood of hyperuricemia model animals, the mistletoe extract has high security and can be used for medicines and health foods for preparing, preventing and treating hyperuricemia and gout.
Description
Technical field:
The present invention relates to biochemical field, relate in particular to Herba Taxilli flavone compound, particularly a kind of Herba Taxilli extract and preparation method thereof and application.
Background technology:
Gout is to increase the metabolic disease that causes by long-term purine metabolism obstacle, blood uric acid; Hyperuricemia is the important biochemical basis of gout; Also be the risk factor of numerous disease simultaneously; Not only can bring out gouty arthritis like hyperuricemia, urinary calculus, urate nephropathy, and also disease such as serum uric acid level rising and abnormalities of sugar/lipid metabolism, obesity is closely related.
The past primary disease is relatively more rare in Asia or Asians; Much more comparatively to see the America and Europe; But after the nineties in 20th century, regional economy such as Japan, Taiwan develop rapidly, and gout and hyperuricemia become commonly encountered diseases gradually; China the eighties in 20th century future trouble state of an illness condition also be ascendant trend year by year, hyperuricemia/gout has become the important diseases that threatens human health.
Doctor trained in Western medicine mainly contains prevention synthetic medicine of purine (like allopurinol) and uricosuric drainage (probenecid, benzbromarone) to the treatment of hyperuricemia and gout, and toxic and side effects is big, is not suitable for life-time service.Chinese medicine/natural drug treatment gout determined curative effect, toxic and side effects is little, has caused people's extensive concern in recent years.
Summary of the invention:
The object of the present invention is to provide a kind of Herba Taxilli extract, the poisonous side effect of medicine that described this Herba Taxilli extract will solve treatment hyperuricemia of the prior art and gout is big, is not suitable for the technical problem of life-time service.
This Herba Taxilli extract of the present invention; Dry stem and leaf by Herba Taxilli is a raw material, prepares through extraction, purification, calculates with avicularin, Quercetin and three flavone compounds of Quercitroside; In described extract, the percentage by weight of described flavone compound is greater than 50%.
The present invention also provides the method for preparing of above-mentioned Herba Taxilli extract; The step that comprises the dry stem and leaf reflux, extract, of a Herba Taxilli; Also comprise a step that concentrates, filters reflux extracting liquid, also comprising will filtrate for one adopts the step of resin absorption, eluting, comprises that also one is adopted ethanol elution, spissated step; The step that also comprises a dry Herba Taxilli extract concentrated solution, the total content that obtains flavone is greater than 50% extract.
Further, in the step of reflux, extract,, adopt the alcoholic solution of water or 0%～80% concentration to extract.
Further, in the step that adopts resin absorption, eluting, adopt macroporous resin column chromatography or polyamide column chromatography.
The present invention also provides other a kind of method for preparing of above-mentioned Herba Taxilli extract; The step that comprises the dry stem and leaf reflux, extract, of a Herba Taxilli; Also comprise a step that concentrates, filters reflux extracting liquid, also comprise the step of will filtrate defat, an extraction, also comprise one the spissated step of extract; The step that also comprises a dry Herba Taxilli extract concentrated solution, the total content that obtains flavone is greater than 50% extract.
Further, in the step of reflux, extract,, adopt the alcoholic solution of water or 0%～80% concentration to extract.
Further, in the step of will filtrate defat, an extraction, adopt defat with petroleum ether, adopt ethyl acetate extraction.
The present invention also provides a kind of above-mentioned Herba Taxilli extract in preparation prevention or the medicine of treatment hyperuricemia or the application in the health food.
The present invention also provides a kind of above-mentioned Herba Taxilli extract in preparation prevention or the medicine of treatment gout or the application in the health food.
The Herba Taxilli total flavones is from the dry stem and branch with leaf of Loranthaceae (Loranthaceae) plant Herba Taxilli Taxillus chinensis (DC.) Danser, to extract, separate the effective site that obtains; Its main component is avicularin, Quercetin and Quercitroside chemical compound; This effective site of pharmacological testing proof has uric acid effect in the obvious reduction hyperuricemia model animal blood; Safe, the medicine or the health food that can be used to prepare prevention or treat hyperuricemia and/or gout.
The structure of avicularin, Quercetin and Quercitroside chemical compound is following:
Avicularin
Quercitroside
Quercetin Quercetin
The present invention compares with prior art, and its effect is actively with tangible.Animal test results shows that the Herba Taxilli total flavones has significant reduction effect to uric acid in the hyperuricemia model animal blood, and is safe, can be used for preparing the medicine or the health food of prevention or treatment hyperuricemia and gout.
Description of drawings:
Fig. 1 has shown that in the mice hyperuricemia model after employing low dosage total flavones, high dose total flavones, the allopurinol treatment, each organizes the uric acid level of mice.Compare with model group, have significant difference * p＜0.05 and significant differences * * p＜0.01.
Below specify technical scheme of the present invention, do not limit practical range of the present invention with this through specific embodiment.
The specific embodiment:
Embodiment 1
10 kilograms of Herba Taxilli medical materials, the reflux, extract, of packing into jar was with 80L pure water reflux, extract, 1.5 hours; Filter, filtering residue reuse 40L pure water reflux, extract, 1 hour is filtered; Merge extracted twice liquid, be concentrated into 10L, filter; Filtrating is adsorbed in the D101 resin, removes impurity, 50% ethanol elution of 5 times of column volumes of reuse with 10% ethanol elution of 5 times of column volumes; Eluent concentrates, the dry Herba Taxilli total flavones 350g that gets, and the total content of flavone reaches 58% (in avicularin, Quercetin and Quercitroside).
Embodiment 2
10 kilograms of Herba Taxilli medical materials, the reflux, extract, of packing into jar, 40% alcohol reflux of 80L 1.5 hours; Filter, 40% alcohol reflux of filtering residue reuse 40L 1 hour filters, and merges extracted twice liquid; Concentrate and reclaim ethanol, thin up filters to 10L; Polyamide adsorbent resin on the filtrating is removed impurity, 60% ethanol elution of 5 times of column volumes of reuse with 10% ethanol elution of 5 times of column volumes; Eluent concentrates, the dry Herba Taxilli total flavones 310g that gets, and the total content of flavone reaches 63% (in avicularin, Quercetin and Quercitroside).
Embodiment 3
10 kilograms of Herba Taxilli medical materials, the reflux, extract, of packing into jar was with 80% alcohol reflux of 80L 1.5 hours; Filter, 80% alcohol reflux of filtering residue reuse 40L 1 hour filters; Merge extracted twice liquid, concentrate and reclaim ethanol, after concentrated solution adds 3 times of water gaging dilutions; Add 1/2 amount boiling range and be 60～90 ℃ defat with petroleum ether 3 times, water layer is with 1/2 amount ethyl acetate extraction three times, and extract merges, concentrated; The dry Herba Taxilli total flavones 380g that gets, the total content of flavone can reach 55% (in avicularin, Quercetin and Quercitroside).
Embodiment 4
Experimental model: mice hyperuricemia model.
Experimental technique: get 50 of male mice in kunming, body weight 24 ± 2g is divided into 5 groups at random, is respectively blank control group, model group, positive drug group, medicine high dose group, medicine low dose group.Dosage is respectively: low dosage 20mg/kg, high dose 80mg/kg, positive drug allopurinol 5mg/kg.Positive drug group, medicine high dose group, medicine low dose group are distinguished administration 10 days, every day 1 time.After the last administration 1 hour, except that blank control group, all the other each treated animal lumbar injection oxonic acid potassium salt 300mg/kg cause the mice hyperuricemia.Behind the injection 1h, each treated animal is plucked eyeball and is got blood, gets determination of serum hematuria acid number, carries out group difference property relatively.(product that above-mentioned experiment adopts embodiment 2 to obtain with the Herba Taxilli total flavones)
Experimental procedure:
1. kunming mice, adaptability were cultivated for 1 week (during accomplish grouping, labelling and weigh);
2. sun is distinguished administration 10 days, every day 1 time to group and each drug group; The normal control group gives solvent.
3. the last administration is after 1 hour, and except that blank control group, all the other each treated animal lumbar injection oxonic acid potassium salt 300mg/kg cause the mice hyperuricemia.
4. inject back 1h, mice is plucked eyeball and gets blood, about 1ml.Room temperature leaves standstill more than the 1h, and 4 degree 2500r/min low-temperature centrifugations were got supernatant after 15 minutes, and are frozen in-20 degrees centigrade of refrigerators with the every pipe packing of 200ul.
5. serum uric acid concentration determination.
5.1 get serum as measuring sample;
Establish blank pipe, standard pipe and sample tube 5.2 measure, according to form liquid feeding in two steps;
R1 and R2 are two kinds of reagent in the uric acid reagent box, and R1 is the phosphate buffer of ascorbic acid oxidase, peroxidase and 4-amino-antipyrine; R2 be uricase and N-ethyl-N-(3-sulfopropyl)--phosphate buffer of anisidine.(the uric acid reagent box is available from Shanghai Kehua Dongling Diagnosis Products Co., Ltd., lot number: 20091212).
5.3 each pipe of mixing, 37 ℃ of water-baths were cooled to room temperature after 5 minutes, in spectrophotometer 550nm wavelength colorimetric, with reagent blank pipe school zero, read the light absorption value of sample tube and standard pipe.
5.4, calculate the uric acid concentration of sample, uric acid (mg/L)=sample tube light absorption value/standard pipe light absorption value * 100 (titer content) according to light absorption value
Experimental result: the uric acid level of the low dose group of total flavones, high dose group, positive controls obviously reduces, and compares with model group, has significant difference * p＜0.05 and significant differences * * p＜0.01, and the result is as shown in Figure 1.
Claims (9)
1. Herba Taxilli extract; It is characterized in that: the dry stem and leaf by Herba Taxilli is a raw material; Prepare through extraction, purification; Calculate with avicularin, Quercetin and three flavone compounds of Quercitroside, in described extract, the percentage by weight of described flavone compound is greater than 50%.
2. the method for preparing of the described Herba Taxilli extract of claim 1; It is characterized in that: the step that comprises the dry stem and leaf reflux, extract, of a Herba Taxilli; Also comprise a step that concentrates, filters reflux extracting liquid, also comprising will filtrate for one adopts the step of resin absorption, eluting, comprises that also one is adopted ethanol elution, spissated step; The step that also comprises a dry Herba Taxilli extract concentrated solution, the total content that obtains flavone is greater than 50% extract.
3. Herba Taxilli extract method for preparing as claimed in claim 2 is characterized in that: in the step of reflux, extract,, adopt the alcoholic solution of water or 0%～80% concentration to extract.
4. Herba Taxilli extract method for preparing as claimed in claim 2 is characterized in that: in the step that adopts resin absorption, eluting, adopt macroporous resin column chromatography or polyamide column chromatography.
5. the described Herba Taxilli extract method for preparing of claim 1; It is characterized in that: the step that comprises the dry stem and leaf reflux, extract, of a Herba Taxilli; Also comprise a step that concentrates, filters reflux extracting liquid, also comprise the step of will filtrate defat, an extraction, also comprise one the spissated step of extract; The step that also comprises a dry Herba Taxilli extract concentrated solution, the total content that obtains flavone is greater than 50% extract.
6. Herba Taxilli extract method for preparing as claimed in claim 5 is characterized in that: in the step of reflux, extract,, adopt the alcoholic solution of water or 0%～80% concentration to extract.
7. Herba Taxilli extract method for preparing as claimed in claim 5 is characterized in that: in the step of described will filtrate defat, extraction, adopt defat with petroleum ether, adopt ethyl acetate extraction.
8. the described Herba Taxilli extract of claim 1 is in preparation prevention or the medicine of treatment hyperuricemia or the application in the health food.
9. the described Herba Taxilli extract of claim 1 is in preparation prevention or the medicine of treatment gout or the application in the health food.
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| CN2010102609834A CN102370674A (en) | 2010-08-24 | 2010-08-24 | Mistletoe extract, its preparation method and its application |
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| CN2010102609834A CN102370674A (en) | 2010-08-24 | 2010-08-24 | Mistletoe extract, its preparation method and its application |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107467669A (en) * | 2017-09-05 | 2017-12-15 | 山东健康源生物工程有限公司 | A kind of ammonia sugar calcium health products and preparation method thereof |
| CN108379312A (en) * | 2018-05-25 | 2018-08-10 | 云南中医学院 | Application of the Scurrula Parasitica L in preparing gout protective agents or health products |
| CN112322422A (en) * | 2020-11-25 | 2021-02-05 | 梧州学院 | Parasitic loranthus rice wine and brewing method thereof |
| CN112471509A (en) * | 2020-11-25 | 2021-03-12 | 梧州学院 | Loranthus parasiticus syrup with high quercetin content and preparation method thereof |
| CN117867609A (en) * | 2024-02-28 | 2024-04-12 | 梅州鼎泰电路板有限公司 | Copper electroplating process of circuit board |
-
2010
- 2010-08-24 CN CN2010102609834A patent/CN102370674A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 吕琳等: "桑寄生中槲皮素、槲皮苷的鉴定与含量测定", 《中成药》 * |
| 黎莉等: "中草药来源的黄嘌呤氧化酶抑制剂的研究进展", 《中药材》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107467669A (en) * | 2017-09-05 | 2017-12-15 | 山东健康源生物工程有限公司 | A kind of ammonia sugar calcium health products and preparation method thereof |
| CN108379312A (en) * | 2018-05-25 | 2018-08-10 | 云南中医学院 | Application of the Scurrula Parasitica L in preparing gout protective agents or health products |
| CN112322422A (en) * | 2020-11-25 | 2021-02-05 | 梧州学院 | Parasitic loranthus rice wine and brewing method thereof |
| CN112471509A (en) * | 2020-11-25 | 2021-03-12 | 梧州学院 | Loranthus parasiticus syrup with high quercetin content and preparation method thereof |
| CN117867609A (en) * | 2024-02-28 | 2024-04-12 | 梅州鼎泰电路板有限公司 | Copper electroplating process of circuit board |
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Application publication date: 20120314 |